AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045402

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045402 MtrunA17_Chr4g0062881 93.217 457 31 0 297 753 1 457 0.0 871
MsaG045402 MtrunA17_Chr1g0171711 38.662 807 420 20 1 753 1 786 1.04e-148 453
MsaG045402 MtrunA17_Chr1g0148791 50.708 424 173 9 365 753 480 902 2.14e-121 385
MsaG045402 MtrunA17_Chr1g0148791 37.344 241 134 7 132 365 182 412 1.32e-28 122
MsaG045402 MtrunA17_Chr1g0148791 46.067 89 46 2 1 88 1 88 9.47e-17 85.1
MsaG045402 MtrunA17_Chr7g0237271 33.913 805 440 25 8 753 13 784 2.84e-120 379
MsaG045402 MtrunA17_Chr3g0138311 49.296 426 174 10 365 753 477 897 3.61e-119 379
MsaG045402 MtrunA17_Chr3g0138311 36.100 241 140 7 132 365 171 404 2.28e-28 122
MsaG045402 MtrunA17_Chr7g0237241 33.875 800 438 26 8 753 13 775 1.90e-118 374
MsaG045402 MtrunA17_Chr4g0062891 88.947 190 21 0 13 202 22 211 1.28e-117 352
MsaG045402 MtrunA17_Chr2g0282401 41.611 298 163 6 461 753 497 788 3.42e-66 234
MsaG045402 MtrunA17_Chr4g0047051 36.237 287 176 6 471 753 25 308 4.15e-48 172
MsaG045402 MtrunA17_Chr4g0047091 35.764 288 174 8 472 753 84 366 9.54e-42 156
MsaG045402 MtrunA17_Chr4g0047071 33.684 285 171 8 482 753 3 282 7.43e-38 142
MsaG045402 MtrunA17_Chr4g0047061 36.290 248 145 8 512 753 90 330 1.92e-36 140
MsaG045402 MtrunA17_Chr4g0047101 37.118 229 120 8 482 702 32 244 3.62e-31 123
MsaG045402 MtrunA17_Chr5g0405851 29.470 302 178 12 478 752 156 449 6.70e-22 99.8
MsaG045402 MtrunA17_Chr3g0093681 28.369 282 184 9 482 752 278 552 8.58e-22 100
MsaG045402 MtrunA17_Chr1g0172501 28.369 282 184 9 482 752 278 552 2.73e-21 98.6
MsaG045402 MtrunA17_Chr6g0448921 26.210 248 173 6 473 714 13 256 1.62e-17 87.4
MsaG045402 MtrunA17_Chr4g0047111 32.886 149 71 5 557 702 5 127 4.48e-17 79.0
MsaG045402 MtrunA17_Chr3g0136971 28.899 218 141 7 476 687 419 628 1.64e-16 84.0
MsaG045402 MtrunA17_Chr1g0185501 23.427 286 204 10 479 752 30 312 2.03e-14 75.1
MsaG045402 MtrunA17_Chr2g0281451 23.715 253 183 6 468 714 7 255 5.36e-13 72.8
MsaG045402 MtrunA17_Chr4g0067151 25.764 229 152 6 506 719 15 240 1.08e-12 70.1
MsaG045402 MtrunA17_Chr4g0067151 27.136 199 131 6 484 671 86 281 1.33e-12 69.7
MsaG045402 MtrunA17_Chr4g0067151 25.980 204 139 6 476 668 120 322 8.70e-12 67.0
MsaG045402 MtrunA17_Chr8g0392701 26.423 246 159 11 490 720 30 268 1.28e-11 66.6
MsaG045402 MtrunA17_Chr2g0308391 22.159 352 231 12 435 753 91 432 2.59e-11 66.6
MsaG045402 MtrunA17_Chr1g0168391 24.865 185 131 5 490 667 238 421 2.84e-11 66.6
MsaG045402 MtrunA17_Chr2g0295791 23.551 276 195 8 491 753 185 457 5.48e-11 65.9
MsaG045402 MtrunA17_Chr4g0035611 22.183 284 181 10 479 727 54 332 5.74e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045402 AT2G32700.6 36.946 812 426 26 1 753 1 785 7.64e-135 418
MsaG045402 AT2G32700.2 36.855 814 426 27 1 753 1 787 3.60e-133 413
MsaG045402 AT2G32700.5 36.855 814 426 27 1 753 1 787 3.60e-133 413
MsaG045402 AT2G32700.1 36.855 814 426 27 1 753 1 787 3.60e-133 413
MsaG045402 AT2G32700.4 36.855 814 426 27 1 753 1 787 3.60e-133 413
MsaG045402 AT2G32700.3 36.855 814 426 27 1 753 1 787 3.60e-133 413
MsaG045402 AT2G32700.7 36.386 830 427 27 1 753 1 806 3.23e-132 411
MsaG045402 AT4G32551.1 49.517 414 180 7 368 753 519 931 2.43e-122 389
MsaG045402 AT4G32551.1 36.111 252 147 8 116 361 195 438 1.17e-28 123
MsaG045402 AT4G32551.1 47.191 89 45 2 1 88 1 88 2.43e-17 87.4
MsaG045402 AT4G32551.2 49.517 414 180 7 368 753 557 969 9.93e-122 389
MsaG045402 AT4G32551.2 31.724 290 146 9 116 361 195 476 1.64e-23 107
MsaG045402 AT4G32551.2 47.191 89 45 2 1 88 1 88 2.43e-17 87.4
MsaG045402 AT2G41500.1 29.787 282 180 9 482 752 261 535 2.83e-22 102
MsaG045402 AT2G41500.2 29.787 282 180 9 482 752 261 535 2.83e-22 102
MsaG045402 AT1G61210.3 25.483 259 180 7 462 714 4 255 1.36e-17 88.2
MsaG045402 AT1G61210.3 23.744 219 149 7 481 684 105 320 4.65e-11 67.0
MsaG045402 AT1G61210.1 25.483 259 180 7 462 714 4 255 1.47e-17 88.2
MsaG045402 AT1G61210.1 23.744 219 149 7 481 684 105 320 6.02e-11 66.6
MsaG045402 AT1G61210.2 25.483 259 180 7 462 714 4 255 1.63e-17 88.2
MsaG045402 AT1G61210.2 23.744 219 149 7 481 684 105 320 6.39e-11 66.6
MsaG045402 AT5G25150.1 29.091 220 144 5 473 687 415 627 4.60e-17 85.9
MsaG045402 AT5G25150.2 29.864 221 141 7 473 687 415 627 4.73e-17 85.9
MsaG045402 AT5G23430.3 27.092 251 167 7 473 714 13 256 8.08e-17 85.5
MsaG045402 AT5G23430.2 27.092 251 167 7 473 714 13 256 8.08e-17 85.5
MsaG045402 AT5G23430.1 27.092 251 167 7 473 714 13 256 9.04e-17 85.5
MsaG045402 AT5G08390.1 26.295 251 169 7 473 714 13 256 4.08e-16 83.2
MsaG045402 AT3G49660.1 24.658 292 196 12 479 752 27 312 1.34e-15 79.0
MsaG045402 AT3G49660.1 24.514 257 174 10 513 753 19 271 2.71e-13 72.0
MsaG045402 AT4G02730.1 23.875 289 202 9 479 753 46 330 2.79e-15 78.2
MsaG045402 AT1G11160.1 23.552 259 185 7 462 714 4 255 9.35e-15 79.0
MsaG045402 AT1G11160.1 25.000 192 138 4 481 668 105 294 3.78e-11 67.4
MsaG045402 AT1G11160.2 23.552 259 185 7 462 714 4 255 1.02e-14 79.0
MsaG045402 AT1G11160.2 25.000 192 138 4 481 668 105 294 3.81e-11 67.4
MsaG045402 AT1G11160.4 23.552 259 185 7 462 714 4 255 1.16e-14 78.6
MsaG045402 AT1G11160.4 25.000 192 138 4 481 668 105 294 3.82e-11 67.4
MsaG045402 AT5G60940.1 23.934 305 198 11 479 753 126 426 1.51e-13 73.9
MsaG045402 AT5G13480.1 23.297 279 199 9 484 752 131 404 3.29e-13 73.6
MsaG045402 AT5G13480.4 23.297 279 199 9 484 752 119 392 3.59e-13 73.6
MsaG045402 AT5G13480.3 23.297 279 199 9 484 752 119 392 3.77e-13 73.6
MsaG045402 AT5G60940.2 23.934 305 198 11 479 753 34 334 3.98e-13 72.0
MsaG045402 AT5G13480.2 23.297 279 199 9 484 752 142 415 4.83e-13 73.2
MsaG045402 AT2G33340.2 25.668 187 129 4 490 667 236 421 1.32e-12 71.6
MsaG045402 AT2G33340.1 25.668 187 129 4 490 667 236 421 1.32e-12 71.6
MsaG045402 AT2G33340.3 25.668 187 129 4 490 667 236 421 1.36e-12 71.2
MsaG045402 AT5G08560.1 23.904 251 175 8 514 752 270 516 1.13e-11 68.6
MsaG045402 AT5G08560.2 23.904 251 175 8 514 752 270 516 1.13e-11 68.6
MsaG045402 AT5G08560.3 23.904 251 175 8 514 752 270 516 1.13e-11 68.6
MsaG045402 AT1G11160.3 25.000 192 138 4 481 668 63 252 3.98e-11 67.4
MsaG045402 AT5G67320.1 27.778 162 104 4 478 627 450 610 7.43e-11 66.2
MsaG045402 AT2G43770.1 20.280 286 204 11 484 751 61 340 8.92e-11 64.7

Find 181 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTCTTCGGACATTTAC+AGG 0.170744 8:-72379764 MsaT045402.1:CDS
GCTCTTCAAGCGTAGATTTC+AGG 0.213054 8:+72380632 None:intergenic
CACCATAGGAGCATTGTTTA+TGG 0.233609 8:+72384637 None:intergenic
CTTCCGGTCAACTTGACTTT+TGG 0.234717 8:+72381704 None:intergenic
CCTGCTCAGCTGCAGCAATA+TGG 0.235792 8:-72384701 MsaT045402.1:CDS
TATTTGTTCTTGTGATAATA+AGG 0.238048 8:-72379693 MsaT045402.1:CDS
AGTACCATGAACACTGTAAA+TGG 0.257174 8:+72383965 None:intergenic
AAGTCCAGATGAGGCAATTC+AGG 0.270725 8:+72381884 None:intergenic
TCAGTGTGCTGGCACTCTTC+TGG 0.277434 8:-72379087 MsaT045402.1:CDS
GCGATTTCATACGCTTTATC+AGG 0.280157 8:+72386414 None:intergenic
GAACAAATGTAATACCTTAA+AGG 0.280380 8:+72384520 None:intergenic
GCTTGTCCATAATCAAAATA+TGG 0.283609 8:-72381984 MsaT045402.1:CDS
TTGTGCAGGTGTTACAATAT+TGG 0.283850 8:+72381916 None:intergenic
TGTGCAGGTGTTACAATATT+GGG 0.283894 8:+72381917 None:intergenic
CTGCTCAGCTGCAGCAATAT+GGG 0.293474 8:-72384700 MsaT045402.1:CDS
CCATATTGCTGCAGCTGAGC+AGG 0.294656 8:+72384701 None:intergenic
GAAGGCTGTCCACCAGTTCT+TGG 0.309028 8:+72384344 None:intergenic
GATGGAAAACTATTTGTTAC+TGG 0.314915 8:-72380827 MsaT045402.1:CDS
TACTGGTGGTCGTGATAAAA+AGG 0.316571 8:-72380810 MsaT045402.1:intron
TTGCAAATGGTAGTTGTATT+GGG 0.320162 8:-72379652 MsaT045402.1:CDS
TTTCCCTGAATTGCCTCATC+TGG 0.320474 8:-72381888 MsaT045402.1:CDS
CAGGTTGAAGACTCCCCTTC+AGG 0.322856 8:-72384564 MsaT045402.1:CDS
TGTTATTTGGCAGGCTCATA+TGG 0.330746 8:-72381162 MsaT045402.1:intron
AGAACTGGTGGACAGCCTTC+AGG 0.335242 8:-72384341 MsaT045402.1:CDS
ATTGCAAATGGTAGTTGTAT+TGG 0.340157 8:-72379653 MsaT045402.1:CDS
CTCCTATGGTGAGGCAGAAC+CGG 0.346361 8:-72384625 MsaT045402.1:CDS
ATTGTAACACCTGCACAAAT+TGG 0.348653 8:-72381911 MsaT045402.1:CDS
TAATTCAGTCGCTTATGCTC+TGG 0.350259 8:-72378812 MsaT045402.1:intron
GTGAGATTTCAACCTGGTTT+GGG 0.350453 8:-72379513 MsaT045402.1:CDS
CAAATAAGGCCACGTTTACT+TGG 0.352863 8:+72379710 None:intergenic
TCTCAAGAGCAACGGATCTT+TGG 0.353331 8:-72381135 MsaT045402.1:CDS
TATTATCACAAGAACAAATA+AGG 0.354896 8:+72379696 None:intergenic
ATGGCAGCAACTTATGTTTC+AGG 0.355840 8:-72382065 MsaT045402.1:CDS
GCACTCTTCTGGAAATTATC+TGG 0.356961 8:-72379076 MsaT045402.1:CDS
CCGATAAAACTGTAAAGGTT+TGG 0.363852 8:-72380546 MsaT045402.1:CDS
GGTGAGATTTCAACCTGGTT+TGG 0.364221 8:-72379514 MsaT045402.1:CDS
TTTATCAGGTTCATCGCATT+TGG 0.370622 8:+72386428 None:intergenic
GGCAATGTTGAACAGAATAT+TGG 0.370914 8:-72385662 MsaT045402.1:intron
AGCCAAAGTCTTACTGCTAA+TGG 0.374190 8:+72382996 None:intergenic
ATAATAAGGAAATAAGATAT+TGG 0.375637 8:-72379679 MsaT045402.1:CDS
AATCAAAACCAGCAACTTCC+AGG 0.378139 8:-72384148 MsaT045402.1:CDS
CTTGTAACCCTGGAAGTTGC+TGG 0.378969 8:+72384140 None:intergenic
AACACAATGGGATCATCTTC+TGG 0.380898 8:-72381627 MsaT045402.1:CDS
ACCATAGGAGCATTGTTTAT+GGG 0.382702 8:+72384638 None:intergenic
CTTGGTCAACTCGTCCATTA+TGG 0.384812 8:+72381072 None:intergenic
CATATTGCTGCAGCTGAGCA+GGG 0.392189 8:+72384702 None:intergenic
CGATAAAACTGTAAAGGTTT+GGG 0.397292 8:-72380545 MsaT045402.1:CDS
TGCAGCCTGGTCATTCTCTT+CGG 0.397318 8:-72379775 MsaT045402.1:intron
GTTTGCAGTTCCAGAGCTAT+TGG 0.397684 8:+72381647 None:intergenic
ACCAGCATTATCTTACCTTA+AGG 0.398546 8:-72385442 MsaT045402.1:intron
AAGGTTGGTTGATACCTTAT+GGG 0.400769 8:+72381853 None:intergenic
GGATCCATTTACAGTGTTCA+TGG 0.401695 8:-72383969 MsaT045402.1:intron
ACTTGGCAACAGAATGGTTT+CGG 0.403735 8:-72381567 MsaT045402.1:CDS
CCATCAAAACCACTGACAAA+TGG 0.409375 8:+72382022 None:intergenic
CAGGGCTCTGCAATATGAAC+TGG 0.414323 8:+72384720 None:intergenic
GGCATCTGTTTGTAATGATC+TGG 0.417806 8:-72379055 MsaT045402.1:CDS
GAGTTGCAGATTTAAATTGC+AGG 0.422031 8:-72379433 MsaT045402.1:intron
CTGCCATGGTGAAAATTGAT+TGG 0.424275 8:+72382081 None:intergenic
CTTGCTGATTCAGACCATAA+TGG 0.437173 8:-72381086 MsaT045402.1:CDS
ATGATAGGACTTTCAAGATC+TGG 0.438220 8:-72378689 MsaT045402.1:CDS
AATTCAGTCGCTTATGCTCT+GGG 0.438597 8:-72378811 MsaT045402.1:intron
TTTACAAGAGAAACTTCAAC+AGG 0.441555 8:-72384176 MsaT045402.1:CDS
ATGAGATTCAGGCTTCTCTA+TGG 0.444675 8:-72380672 MsaT045402.1:intron
CTCTGGGCCATTGGATCTGA+TGG 0.445049 8:-72379027 MsaT045402.1:CDS
CCATTGGATCTGATGGCAGC+GGG 0.448766 8:-72379020 MsaT045402.1:CDS
GAACTGGTGGACAGCCTTCA+GGG 0.451874 8:-72384340 MsaT045402.1:intron
GCTTATGCTCTGGGACTTTG+AGG 0.454130 8:-72378802 MsaT045402.1:CDS
TGCTCAGCTGCAGCAATATG+GGG 0.454725 8:-72384699 MsaT045402.1:CDS
TTGGCAGTATCAGATGTTAC+TGG 0.456091 8:-72378732 MsaT045402.1:CDS
TGGCTTCAGTTAGTCATGAT+AGG 0.456464 8:-72378704 MsaT045402.1:CDS
TAATGATCTGGTTAAACTCT+GGG 0.456770 8:-72379043 MsaT045402.1:CDS
TCCCATAAACAATGCTCCTA+TGG 0.458377 8:-72384639 MsaT045402.1:CDS
GCCTTGTTCGTTCAGTGTGC+TGG 0.458741 8:-72379098 MsaT045402.1:CDS
AACAAGGCACAATCCTCGTT+TGG 0.460063 8:+72384383 None:intergenic
CTGGTTAAACTCTGGGCCAT+TGG 0.460194 8:-72379036 MsaT045402.1:CDS
GTAATGATCTGGTTAAACTC+TGG 0.461731 8:-72379044 MsaT045402.1:CDS
TGCACTCATGCCACTGTGCA+TGG 0.464962 8:+72384022 None:intergenic
TGATTATGGACAAGCAAACT+TGG 0.465215 8:+72381992 None:intergenic
AACCACCTACAGAATTTGAT+TGG 0.466272 8:+72381945 None:intergenic
ATAATCAAAATATGGTTCCT+TGG 0.467781 8:-72381976 MsaT045402.1:CDS
TCAGGTCTTGCCAATAGCTC+TGG 0.469604 8:-72381657 MsaT045402.1:CDS
CGAAAAGTAGGATCAAGTTC+AGG 0.470472 8:-72381675 MsaT045402.1:CDS
TCAAAATATTGTAGCTAGGA+TGG 0.471613 8:+72378944 None:intergenic
TGCAGACACGACTGCCAACT+TGG 0.472292 8:-72381584 MsaT045402.1:CDS
GGCAGTCGTGTCTGCATAGT+TGG 0.473371 8:+72381591 None:intergenic
TTCTGTTTGCAGACAAACAT+TGG 0.483931 8:-72384215 MsaT045402.1:intron
GCAAGAATATTTCATGATGA+AGG 0.484394 8:-72385683 MsaT045402.1:CDS
ACTTATGTTTCAGGCACAGC+AGG 0.484632 8:-72382056 MsaT045402.1:CDS
ATCAGATGTTACTGGATTGG+TGG 0.488563 8:-72378724 MsaT045402.1:CDS
AAACCATTCTGTTGCCAAGT+TGG 0.494388 8:+72381570 None:intergenic
AACGAAATAGCACCAAGAAC+TGG 0.496303 8:-72384356 MsaT045402.1:CDS
GCCATTGGATCTGATGGCAG+CGG 0.497982 8:-72379021 MsaT045402.1:CDS
TCCTATGGTGAGGCAGAACC+GGG 0.500407 8:-72384624 MsaT045402.1:CDS
AGATATTGGAATATTGCAAA+TGG 0.505994 8:-72379665 MsaT045402.1:CDS
TGCATCAACACAGAATCAAT+TGG 0.506774 8:-72381538 MsaT045402.1:intron
TCTGGAACTGCAAACACAAT+GGG 0.508959 8:-72381639 MsaT045402.1:CDS
TAATGAGATCAAGCATGGAT+TGG 0.510535 8:-72380888 MsaT045402.1:CDS
GATACAAGCTTGTCGTGTGC+AGG 0.517006 8:+72378759 None:intergenic
TGTTGTCACTTCTCCTCGGA+TGG 0.520645 8:-72380845 MsaT045402.1:CDS
CTGCCAACTTGGCAACAGAA+TGG 0.522284 8:-72381573 MsaT045402.1:CDS
TTGTAAAATGCTAAGTGCAC+TGG 0.525997 8:+72384192 None:intergenic
CCCGCTGCCATCAGATCCAA+TGG 0.526518 8:+72379020 None:intergenic
TCATTTAATGAGATCAAGCA+TGG 0.527559 8:-72380893 MsaT045402.1:CDS
ATTTCAACCTGGTTTGGGAA+AGG 0.527606 8:-72379508 MsaT045402.1:CDS
TAGCAACATAGAACATGCTT+GGG 0.529135 8:+72380578 None:intergenic
TCAGGGAAACCAATTTGTGC+AGG 0.529698 8:+72381902 None:intergenic
CAAGAGCAACGGATCTTTGG+CGG 0.529755 8:-72381132 MsaT045402.1:CDS
GCAAGAACCTTTCCCAAACC+AGG 0.531493 8:+72379501 None:intergenic
CATACAATTGATTCTTCATG+AGG 0.532585 8:+72385711 None:intergenic
GTTGTTCTCTCTTCGAGTGG+TGG 0.533117 8:-72385515 MsaT045402.1:CDS
AGTCCAGATGAGGCAATTCA+GGG 0.534232 8:+72381885 None:intergenic
ATGATCAAAATATTGTAGCT+AGG 0.535188 8:+72378940 None:intergenic
CTCAGCCGATAAAACTGTAA+AGG 0.537151 8:-72380551 MsaT045402.1:CDS
CATCAAAAGCATTTCCCTGA+AGG 0.539630 8:+72384549 None:intergenic
GTAGCAACATAGAACATGCT+TGG 0.539825 8:+72380577 None:intergenic
TGCTATTTCGTTCAACAACA+AGG 0.543891 8:+72384367 None:intergenic
CTCGTTTGGAAGCTGACCGC+GGG 0.545244 8:+72384397 None:intergenic
CCGGGTTACTTCTAATACAA+TGG 0.545781 8:-72384606 MsaT045402.1:CDS
AATGGCAACAAAACTAAATG+AGG 0.546151 8:-72384588 MsaT045402.1:CDS
GGACTTAGATATGACCCATA+AGG 0.547552 8:-72381867 MsaT045402.1:intron
CCATTGTATTAGAAGTAACC+CGG 0.548960 8:+72384606 None:intergenic
CGACAAGCTTGTATCTGCAT+TGG 0.551855 8:-72378751 MsaT045402.1:CDS
TCTCAAGCTGTTGTTTCAGA+AGG 0.560493 8:-72383990 MsaT045402.1:CDS
TTTGATTGGCCATCGTTCCA+AGG 0.560547 8:+72381959 None:intergenic
GTCTGCATAGTTGGTGTAGA+GGG 0.561086 8:+72381600 None:intergenic
CTTCCAAAAGTCAAGTTGAC+CGG 0.561136 8:-72381707 MsaT045402.1:CDS
TGACTGTTGTCACTTCTCCT+CGG 0.561258 8:-72380849 MsaT045402.1:CDS
AGGTTGAAGACTCCCCTTCA+GGG 0.566579 8:-72384563 MsaT045402.1:CDS
GGTAGCGACTTGACTCTGAA+AGG 0.568593 8:-72383023 MsaT045402.1:CDS
GTAGCGACTTGACTCTGAAA+GGG 0.570038 8:-72383022 MsaT045402.1:CDS
ATCAAAAGCATTTCCCTGAA+GGG 0.572579 8:+72384550 None:intergenic
CTCTGGAACTGCAAACACAA+TGG 0.574440 8:-72381640 MsaT045402.1:CDS
TTATCAGGGTGGTGTAACTC+AGG 0.575396 8:-72379535 MsaT045402.1:intron
CTGGTTGTTCTCTCTTCGAG+TGG 0.576679 8:-72385518 MsaT045402.1:CDS
CAACAAAACTAAATGAGGAC+AGG 0.577019 8:-72384583 MsaT045402.1:CDS
ACCTTAAGGTAAGATAATGC+TGG 0.580228 8:+72385441 None:intergenic
CGCTTGAAGAGCACACTCAA+CGG 0.586936 8:-72380621 MsaT045402.1:CDS
TTTACGATTACTTGTAACCC+TGG 0.588248 8:+72384130 None:intergenic
GAATTGTTTATGGCCAAACA+AGG 0.589606 8:-72385480 MsaT045402.1:CDS
CTGAACGAACAAGGCTCATG+TGG 0.591408 8:+72379106 None:intergenic
TTAGTTAATCCATTTGTCAG+TGG 0.592463 8:-72382031 MsaT045402.1:CDS
AAGATCCGTTGCTCTTGAGA+TGG 0.593548 8:+72381138 None:intergenic
AGTATCAGATGTTACTGGAT+TGG 0.595920 8:-72378727 MsaT045402.1:CDS
AATATGGTTCCTTGGAACGA+TGG 0.597683 8:-72381968 MsaT045402.1:CDS
ATTCTCTTCGGACATTTACA+GGG 0.599684 8:-72379763 MsaT045402.1:CDS
ACCCGGTTCTGCCTCACCAT+AGG 0.601611 8:+72384623 None:intergenic
GCCAGCACACTGAACGAACA+AGG 0.603185 8:+72379097 None:intergenic
AGGTGTTACAATATTGGGAA+TGG 0.603227 8:+72381922 None:intergenic
ATCGCATTTGGATTGAGACA+TGG 0.604361 8:+72386440 None:intergenic
TCAAAAGCATTTCCCTGAAG+GGG 0.605318 8:+72384551 None:intergenic
GGAAAACTATTTGTTACTGG+TGG 0.605813 8:-72380824 MsaT045402.1:CDS
ATCAAAACCAGCAACTTCCA+GGG 0.612502 8:-72384147 MsaT045402.1:CDS
TGAAACATAAGTTGCTGCCA+TGG 0.615754 8:+72382067 None:intergenic
GTTTATGGGACAGAGAACAT+TGG 0.619165 8:+72384652 None:intergenic
GGCCAATCAAATTCTGTAGG+TGG 0.625840 8:-72381947 MsaT045402.1:CDS
CCGCGGTCAGCTTCCAAACG+AGG 0.626261 8:-72384396 MsaT045402.1:CDS
TGTCTGCATAGTTGGTGTAG+AGG 0.626946 8:+72381599 None:intergenic
GATGGCCAATCAAATTCTGT+AGG 0.638312 8:-72381950 MsaT045402.1:CDS
CATTGGATCTGATGGCAGCG+GGG 0.639120 8:-72379019 MsaT045402.1:CDS
GAAATAGCACCAAGAACTGG+TGG 0.641067 8:-72384353 MsaT045402.1:CDS
ATGGACCATCTCAAGAGCAA+CGG 0.642745 8:-72381143 MsaT045402.1:CDS
AATTGATTGGAAGATTGTGA+AGG 0.646797 8:+72382094 None:intergenic
ATGATTAGTAGGCACCCTGA+AGG 0.649924 8:+72384326 None:intergenic
GCGACTTGACTCTGAAAGGG+TGG 0.650076 8:-72383019 MsaT045402.1:CDS
GTCATATCTAAGTCCAGATG+AGG 0.650884 8:+72381875 None:intergenic
GGGATTGGATGAGCATCATG+CGG 0.660736 8:-72382146 MsaT045402.1:CDS
TAAACAATGCTCCTATGGTG+AGG 0.661726 8:-72384634 MsaT045402.1:CDS
CCTCGTTTGGAAGCTGACCG+CGG 0.663224 8:+72384396 None:intergenic
TTTGATCATTTGCTGCAGTG+AGG 0.663983 8:-72378926 MsaT045402.1:intron
AAATTTAGGCTCAAGAAATG+CGG 0.670041 8:-72384769 MsaT045402.1:intron
TATGGGGCTGAAACTCAAGA+AGG 0.670598 8:-72384683 MsaT045402.1:CDS
ACTCAGGTGAGATTTCAACC+TGG 0.671694 8:-72379519 MsaT045402.1:CDS
AATGTCCGAAGAGAATGACC+AGG 0.680463 8:+72379770 None:intergenic
CACTCGAAGAGAGAACAACC+AGG 0.686015 8:+72385519 None:intergenic
GACTTTCATCCAAGTAAACG+TGG 0.686140 8:-72379719 MsaT045402.1:CDS
GGTGTAGTTATGGATGCACC+TGG 0.690437 8:-72385537 MsaT045402.1:intron
CATAATGGACGAGTTGACCA+AGG 0.691350 8:-72381071 MsaT045402.1:intron
GTGGCATGAGTGCAATGATG+AGG 0.712221 8:-72384013 MsaT045402.1:CDS
CTGAATCAGGCCATGCACAG+TGG 0.713979 8:-72384032 MsaT045402.1:intron
ATAATGAATGCTTATACCCG+CGG 0.714622 8:-72384413 MsaT045402.1:CDS
GTAGCTAGGATGGAAAACGC+AGG 0.717458 8:+72378954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 72378683 72386460 72378683 ID=MsaG045402
Chr8 mRNA 72378683 72386460 72378683 ID=MsaT045402.1;Parent=MsaG045402
Chr8 exon 72378683 72378826 72378683 ID=MsaT045402.1.exon17;Parent=MsaT045402.1
Chr8 CDS 72378683 72378826 72378683 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72378927 72379130 72378927 ID=MsaT045402.1.exon16;Parent=MsaT045402.1
Chr8 CDS 72378927 72379130 72378927 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72379434 72379550 72379434 ID=MsaT045402.1.exon15;Parent=MsaT045402.1
Chr8 CDS 72379434 72379550 72379434 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72379643 72379792 72379643 ID=MsaT045402.1.exon14;Parent=MsaT045402.1
Chr8 CDS 72379643 72379792 72379643 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72380534 72380683 72380534 ID=MsaT045402.1.exon13;Parent=MsaT045402.1
Chr8 CDS 72380534 72380683 72380534 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72380811 72380920 72380811 ID=MsaT045402.1.exon12;Parent=MsaT045402.1
Chr8 CDS 72380811 72380920 72380811 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72381072 72381171 72381072 ID=MsaT045402.1.exon11;Parent=MsaT045402.1
Chr8 CDS 72381072 72381171 72381072 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72381539 72381730 72381539 ID=MsaT045402.1.exon10;Parent=MsaT045402.1
Chr8 CDS 72381539 72381730 72381539 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72381868 72382167 72381868 ID=MsaT045402.1.exon9;Parent=MsaT045402.1
Chr8 CDS 72381868 72382167 72381868 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72383010 72383059 72383010 ID=MsaT045402.1.exon8;Parent=MsaT045402.1
Chr8 CDS 72383010 72383059 72383010 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72383970 72384045 72383970 ID=MsaT045402.1.exon7;Parent=MsaT045402.1
Chr8 CDS 72383970 72384045 72383970 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72384141 72384225 72384141 ID=MsaT045402.1.exon6;Parent=MsaT045402.1
Chr8 CDS 72384141 72384225 72384141 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72384341 72384435 72384341 ID=MsaT045402.1.exon5;Parent=MsaT045402.1
Chr8 CDS 72384341 72384435 72384341 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72384535 72384783 72384535 ID=MsaT045402.1.exon4;Parent=MsaT045402.1
Chr8 CDS 72384535 72384783 72384535 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72385443 72385552 72385443 ID=MsaT045402.1.exon3;Parent=MsaT045402.1
Chr8 CDS 72385443 72385552 72385443 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72385663 72385757 72385663 ID=MsaT045402.1.exon2;Parent=MsaT045402.1
Chr8 CDS 72385663 72385757 72385663 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Chr8 exon 72386426 72386460 72386426 ID=MsaT045402.1.exon1;Parent=MsaT045402.1
Chr8 CDS 72386426 72386460 72386426 ID=cds.MsaT045402.1;Parent=MsaT045402.1
Gene Sequence

>MsaT045402.1

ATGTCTCAATCCAAATGCGATGAACCTGATAAAGCGTTAGATGCTTTTATCTACGATTACCTCATGAAGAATCAATTGTATGAAACTGCAAGAATATTTCATGATGAAGGCAATGTTGAACAGAATATTGTTATGGATGCACCTGGTTGTTCTCTCTTCGAGTGGTGGTCTGTTTTCTGGGAATTGTTTATGGCCAAACAAGGTTTTTCACTCTCAGAACCAGCATTATCTTACCTTAAGGCTCAAGAAATGCGGAAGCAAGAAGAAGAGATAGAAAAAATCCAGTTCATATTGCAGAGCCCTGCTCAGCTGCAGCAATATGGGGCTGAAACTCAAGAAGGTGTTAATGCCAATGTTCTCTGTCCCATAAACAATGCTCCTATGGTGAGGCAGAACCGGGTTACTTCTAATACAATGGCAACAAAACTAAATGAGGACAGGTTGAAGACTCCCCTTCAGGGAAATGCTTTTGATGACAGGACCTTTAAGATAATGAATGCTTATACCCGCGGTCAGCTTCCAAACGAGGATTGTGCCTTGTTGTTGAACGAAATAGCACCAAGAACTGGTGGACAGCCTTCAGGACAAACATTGGCCAGTGCACTTAGCATTTTACAAGAGAAACTTCAACAGGATCAAAATCAAAACCAGCAACTTCCAGGGTTACAAGCCATGCACAGTGGCATGAGTGCAATGATGAGGTCTCAAGCTGTTGTTTCAGAAGGATCCATTTACAGTGTTCATGGTTCAAATCAAGGTGGTAGCGACTTGACTCTGAAAGGGTGGCCATTAGCAGGATTGGATGAGCATCATGCGGAACTTCTTCATAAGCATAATTTGATTCTGCCTTCACAATCTTCCAATCAATTTTCACCATGGCAGCAACTTATGTTTCAGGCACAGCAGGATTTAGTTAATCCATTTGTCAGTGGTTTTGATGGCAAAAGACCAAGTTTGCTTGTCCATAATCAAAATATGGTTCCTTGGAACGATGGCCAATCAAATTCTGTAGGTGGTTCCATTCCCAATATTGTAACACCTGCACAAATTGGTTTCCCTGAATTGCCTCATCTGGACTTAGATATGACCCATAAGGCTTCCAAAAGTCAAGTTGACCGGAAGCAAAAACGAAAAGTAGGATCAAGTTCAGGTCTTGCCAATAGCTCTGGAACTGCAAACACAATGGGATCATCTTCTGGTTCACCCTCTACACCAACTATGCAGACACGACTGCCAACTTGGCAACAGAATGGTTTCGGCTCACTTGCATCAACACAGAATCAATTGGCTCATATGGACCATCTCAAGAGCAACGGATCTTTGGCGGACAATTTTGAGTTACTCTCATCTCTTGCTGATTCAGACCATAATGGACGAGTTGACCAAGGATTCTCATTTAATGAGATCAAGCATGGATTGGCTAGTTCAGATAAAGTTGACTGTTGTCACTTCTCCTCGGATGGAAAACTATTTGTTACTGGTGGTCGTGATAAAAAGGCTTCTCTATGGTGCACAAAATTGTTCAACCTGAAATCTACGCTTGAAGAGCACACTCAACGGATAACTGATGTTAGATTTAGCCCAAGCATGTTCTATGTTGCTACGTCCTCAGCCGATAAAACTGTAAAGGTTTGGGATGTTAATAATCCTGGTCATTCTCTTCGGACATTTACAGGGAATACAGCTGTGCTGTCACTAGACTTTCATCCAAGTAAACGTGGCCTTATTTGTTCTTGTGATAATAAGGAAATAAGATATTGGAATATTGCAAATGGTAGTTGTATTGGGATTTTCAAGGGTGGTGTAACTCAGGTGAGATTTCAACCTGGTTTGGGAAAGGTTCTTGCAGCAGCAGTAAATAATCAAATACTCATGTTTGACGCAGAAACTCTGAGTTGCAGATTTAAATTGCAGGGCCACATGAGCCTTGTTCGTTCAGTGTGCTGGCACTCTTCTGGAAATTATCTGGCATCTGTTTGTAATGATCTGGTTAAACTCTGGGCCATTGGATCTGATGGCAGCGGGGCATGTATTACGCAAGTGAATGCAGCTGAGAGCAAATTCAAGACCTGCGTTTTCCATCCTAGCTACAATATTTTGATCATTTGCTGCAGTGAGTCGCTTATGCTCTGGGACTTTGAGGAGAGCAAAAAACTTATTGTGCCTGCACACGACAAGCTTGTATCTGCATTGGCAGTATCAGATGTTACTGGATTGGTGGCTTCAGTTAGTCATGATAGGACTTTCAAGATCTGGAAGTGA
Protein sequence

>MsaT045402.1

MSQSKCDEPDKALDAFIYDYLMKNQLYETARIFHDEGNVEQNIVMDAPGCSLFEWWSVFWELFMAKQGFSLSEPALSYLKAQEMRKQEEEIEKIQFILQSPAQLQQYGAETQEGVNANVLCPINNAPMVRQNRVTSNTMATKLNEDRLKTPLQGNAFDDRTFKIMNAYTRGQLPNEDCALLLNEIAPRTGGQPSGQTLASALSILQEKLQQDQNQNQQLPGLQAMHSGMSAMMRSQAVVSEGSIYSVHGSNQGGSDLTLKGWPLAGLDEHHAELLHKHNLILPSQSSNQFSPWQQLMFQAQQDLVNPFVSGFDGKRPSLLVHNQNMVPWNDGQSNSVGGSIPNIVTPAQIGFPELPHLDLDMTHKASKSQVDRKQKRKVGSSSGLANSSGTANTMGSSSGSPSTPTMQTRLPTWQQNGFGSLASTQNQLAHMDHLKSNGSLADNFELLSSLADSDHNGRVDQGFSFNEIKHGLASSDKVDCCHFSSDGKLFVTGGRDKKASLWCTKLFNLKSTLEEHTQRITDVRFSPSMFYVATSSADKTVKVWDVNNPGHSLRTFTGNTAVLSLDFHPSKRGLICSCDNKEIRYWNIANGSCIGIFKGGVTQVRFQPGLGKVLAAAVNNQILMFDAETLSCRFKLQGHMSLVRSVCWHSSGNYLASVCNDLVKLWAIGSDGSGACITQVNAAESKFKTCVFHPSYNILIICCSESLMLWDFEESKKLIVPAHDKLVSALAVSDVTGLVASVSHDRTFKIWK*