Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045467 | XP_003609277.1 | 65.769 | 260 | 34 | 3 | 1 | 206 | 12 | 270 | 1.24e-111 | 330 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045467 | sp|Q5PP65|C3H28_ARATH | 32.474 | 194 | 86 | 8 | 44 | 206 | 71 | 250 | 5.45e-20 | 87.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045467 | tr|G7JVB8|G7JVB8_MEDTR | 65.769 | 260 | 34 | 3 | 1 | 206 | 12 | 270 | 5.93e-112 | 330 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG045467 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045467 | MtrunA17_Chr4g0063371 | 66.210 | 219 | 60 | 4 | 1 | 205 | 12 | 230 | 4.57e-92 | 269 |
| MsaG045467 | MtrunA17_Chr4g0063391 | 65.000 | 200 | 23 | 2 | 1 | 154 | 12 | 210 | 1.16e-85 | 252 |
| MsaG045467 | MtrunA17_Chr4g0063401 | 40.586 | 239 | 54 | 7 | 1 | 153 | 12 | 248 | 7.27e-43 | 144 |
| MsaG045467 | MtrunA17_Chr4g0063361 | 38.865 | 229 | 62 | 5 | 1 | 151 | 12 | 240 | 1.23e-41 | 141 |
| MsaG045467 | MtrunA17_Chr3g0098251 | 36.932 | 176 | 53 | 5 | 20 | 151 | 15 | 176 | 3.35e-24 | 94.7 |
| MsaG045467 | MtrunA17_Chr8g0366501 | 30.120 | 166 | 93 | 3 | 5 | 152 | 36 | 196 | 2.97e-15 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045467 | AT2G35430.1 | 32.474 | 194 | 86 | 8 | 44 | 206 | 71 | 250 | 5.54e-21 | 87.8 |
| MsaG045467 | AT3G19360.1 | 32.927 | 164 | 89 | 5 | 20 | 173 | 81 | 233 | 1.70e-18 | 82.8 |
| MsaG045467 | AT3G19360.1 | 29.851 | 201 | 95 | 7 | 43 | 206 | 182 | 373 | 1.68e-14 | 71.6 |
| MsaG045467 | AT3G19360.2 | 32.927 | 164 | 89 | 5 | 20 | 173 | 81 | 233 | 1.70e-18 | 82.8 |
| MsaG045467 | AT3G19360.2 | 29.851 | 201 | 95 | 7 | 43 | 206 | 182 | 373 | 1.68e-14 | 71.6 |
| MsaG045467 | AT1G32360.1 | 32.727 | 110 | 73 | 1 | 43 | 151 | 90 | 199 | 2.00e-13 | 68.6 |
Find 56 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATTGAAACTAATAATT+TGG | 0.147683 | 8:+73100847 | MsaT045467.1:CDS |
| CCAACTAGCTTCTGCGAATT+TGG | 0.173447 | 8:-73100630 | None:intergenic |
| AGCCAGATCAATCGAATTTA+CGG | 0.290260 | 8:+73102460 | MsaT045467.1:CDS |
| TCAATCGAATTTACGGTGAT+TGG | 0.313071 | 8:+73102467 | MsaT045467.1:CDS |
| ATGAAGATCCTGCACAGTTT+AGG | 0.341189 | 8:+73100786 | MsaT045467.1:intron |
| CCAACTAAGAAGCGTATACT+TGG | 0.350951 | 8:+73102427 | MsaT045467.1:CDS |
| TTCCAAATTATTAGTTTCAA+TGG | 0.356757 | 8:-73100849 | None:intergenic |
| ATTTGTACTAAGTTCAGATT+TGG | 0.359410 | 8:+73100543 | MsaT045467.1:CDS |
| GAAGCACTCTCTTGATTTCA+AGG | 0.359778 | 8:-73100819 | None:intergenic |
| CGACTAAACTGTGTCGCAAT+TGG | 0.369653 | 8:+73100915 | MsaT045467.1:CDS |
| TGAACAACAATGACTTCAAA+AGG | 0.386314 | 8:+73100424 | MsaT045467.1:CDS |
| AAATCAGAGTTACAAGTTCC+TGG | 0.400098 | 8:+73102331 | MsaT045467.1:intron |
| AGATTTGGTGCTTGTAGAAA+CGG | 0.437088 | 8:+73100558 | MsaT045467.1:CDS |
| AACTGCAGCATCAGCTTCAA+TGG | 0.437500 | 8:-73102359 | None:intergenic |
| CTGGTGCTCTTCCTTTGCTA+TGG | 0.445302 | 8:-73102404 | None:intergenic |
| CCAAGTATACGCTTCTTAGT+TGG | 0.448187 | 8:-73102427 | None:intergenic |
| AGCAAGCAAGGGGCACTTAT+CGG | 0.455209 | 8:+73100891 | MsaT045467.1:CDS |
| CACCGTAAATTCGATTGATC+TGG | 0.457971 | 8:-73102462 | None:intergenic |
| CTCTTTGCTCATGGTGAAGA+AGG | 0.463092 | 8:+73100974 | MsaT045467.1:CDS |
| GGCAACTGCAGCCGCTTCAA+TGG | 0.467968 | 8:-73102383 | None:intergenic |
| CTGATCTGTTTGATTGAGGA+AGG | 0.475163 | 8:-73100497 | None:intergenic |
| GAGACAGGATATTGTTCCTA+TGG | 0.480467 | 8:+73100941 | MsaT045467.1:CDS |
| CTCATGGAGCTGAAGAGATA+AGG | 0.489689 | 8:+73100598 | MsaT045467.1:CDS |
| AATAATTGCAACTTTGCTCA+TGG | 0.494959 | 8:+73100582 | MsaT045467.1:CDS |
| GATTGAGGAAGGGTCATCCT+TGG | 0.496966 | 8:-73100486 | None:intergenic |
| CTTGGAGGAGGGCATATCAA+TGG | 0.505795 | 8:-73100468 | None:intergenic |
| AGCTTCTGCGAATTTGGTGG+TGG | 0.508361 | 8:-73100624 | None:intergenic |
| GGAATGTGAACAAGCAAGCA+AGG | 0.508839 | 8:+73100879 | MsaT045467.1:CDS |
| CTAATAATTTGGAAGGCAGC+AGG | 0.521839 | 8:+73100858 | MsaT045467.1:CDS |
| GTATACGCTTCTTAGTTGGC+TGG | 0.524014 | 8:-73102423 | None:intergenic |
| AGGAGGGCATATCAATGGAT+TGG | 0.527979 | 8:-73100463 | None:intergenic |
| CCAAATTCGCAGAAGCTAGT+TGG | 0.528628 | 8:+73100630 | MsaT045467.1:CDS |
| GGAAGGGTCATCCTTGGAGG+AGG | 0.531078 | 8:-73100480 | None:intergenic |
| TATTCATTCCTAAACTGTGC+AGG | 0.531321 | 8:-73100794 | None:intergenic |
| TGTCGCAATTGGAAGGAGAC+AGG | 0.541370 | 8:+73100926 | MsaT045467.1:CDS |
| ACTAGCTTCTGCGAATTTGG+TGG | 0.541605 | 8:-73100627 | None:intergenic |
| ATTGAAACTAATAATTTGGA+AGG | 0.547647 | 8:+73100851 | MsaT045467.1:CDS |
| GAACAACAATGACTTCAAAA+GGG | 0.550138 | 8:+73100425 | MsaT045467.1:CDS |
| AAAGAGACAGTTCTTTCCAT+AGG | 0.554060 | 8:-73100957 | None:intergenic |
| TGAGGAAGGGTCATCCTTGG+AGG | 0.556539 | 8:-73100483 | None:intergenic |
| CATTGATATGCCCTCCTCCA+AGG | 0.556864 | 8:+73100469 | MsaT045467.1:CDS |
| AACAATGACTTCAAAAGGGA+AGG | 0.561307 | 8:+73100429 | MsaT045467.1:CDS |
| TGGTCTGATCTGTTTGATTG+AGG | 0.566957 | 8:-73100501 | None:intergenic |
| AAGAACTGTCTCTTTGCTCA+TGG | 0.580294 | 8:+73100965 | MsaT045467.1:CDS |
| TCAGCTTCAATGGCTCCACC+AGG | 0.589035 | 8:-73102349 | None:intergenic |
| GGCTGCAGTTGCCATAGCAA+AGG | 0.590440 | 8:+73102393 | MsaT045467.1:CDS |
| TAAACTGTGTCGCAATTGGA+AGG | 0.596328 | 8:+73100919 | MsaT045467.1:CDS |
| GAATGTGAACAAGCAAGCAA+GGG | 0.597583 | 8:+73100880 | MsaT045467.1:CDS |
| TGCTGCAGTTGCCATTGAAG+CGG | 0.606900 | 8:+73102372 | MsaT045467.1:CDS |
| TGATCTGTTTGATTGAGGAA+GGG | 0.618914 | 8:-73100496 | None:intergenic |
| GAAGGGTCATCCTTGGAGGA+GGG | 0.622595 | 8:-73100479 | None:intergenic |
| CAACTAAGAAGCGTATACTT+GGG | 0.638379 | 8:+73102428 | MsaT045467.1:CDS |
| TTGTTCAATGAAGTGAATTG+CGG | 0.641834 | 8:-73100408 | None:intergenic |
| AATGTGAACAAGCAAGCAAG+GGG | 0.656040 | 8:+73100881 | MsaT045467.1:CDS |
| TTCAATGAAGTGAATTGCGG+CGG | 0.657596 | 8:-73100405 | None:intergenic |
| TCAGAGTTACAAGTTCCTGG+TGG | 0.718686 | 8:+73102334 | MsaT045467.1:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 73100399 | 73102501 | 73100399 | ID=MsaG045467 |
| Chr8 | mRNA | 73100399 | 73102501 | 73100399 | ID=MsaT045467.1;Parent=MsaG045467 |
| Chr8 | exon | 73100399 | 73100651 | 73100399 | ID=MsaT045467.1.exon1;Parent=MsaT045467.1 |
| Chr8 | CDS | 73100399 | 73100651 | 73100399 | ID=cds.MsaT045467.1;Parent=MsaT045467.1 |
| Chr8 | exon | 73100792 | 73100995 | 73100792 | ID=MsaT045467.1.exon2;Parent=MsaT045467.1 |
| Chr8 | CDS | 73100792 | 73100995 | 73100792 | ID=cds.MsaT045467.1;Parent=MsaT045467.1 |
| Chr8 | exon | 73102338 | 73102501 | 73102338 | ID=MsaT045467.1.exon3;Parent=MsaT045467.1 |
| Chr8 | CDS | 73102338 | 73102501 | 73102338 | ID=cds.MsaT045467.1;Parent=MsaT045467.1 |
| Gene Sequence |
| Protein sequence |
