AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045473

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045473 MtrunA17_Chr4g0063461 92.500 600 31 2 1 592 1 594 0.0 1106
MsaG045473 MtrunA17_Chr8g0375251 60.331 121 31 1 7 110 117 237 3.12e-44 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045473 AT2G02160.1 54.795 146 50 5 2 131 25 170 2.65e-40 156

Find 131 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGTTGGTATTGGTTATC+TGG 0.119856 8:+73157598 MsaT045473.1:CDS
CACCGATTGCGTTTATTTCT+TGG 0.184040 8:+73157236 MsaT045473.1:CDS
TTGCTTCAAAATTTCTTCTT+TGG 0.213240 8:+73159152 MsaT045473.1:CDS
CTTGCCTTTGATGCCTTTCC+CGG 0.220942 8:-73159761 None:intergenic
TTTGCAGAGTCATTCTTTAA+AGG 0.222829 8:-73158799 None:intergenic
TTGCTTCACAATTGGTTTCC+TGG 0.230232 8:-73159964 None:intergenic
TTTGCAGAGTCATTCTTTAA+AGG 0.231560 8:-73158823 None:intergenic
TTAATATCGGACAAAGCTTT+TGG 0.255089 8:-73159069 None:intergenic
AATAAACCCAACTTAGAAAA+TGG 0.256208 8:+73158970 MsaT045473.1:CDS
CATTCAGAGGTTTGGGATCC+TGG 0.288878 8:-73160103 None:intergenic
GCTTTCTTGGCCTACTTTGC+TGG 0.293087 8:-73159893 None:intergenic
CCAACAAGCAAAGGTTATTC+AGG 0.302583 8:+73159865 MsaT045473.1:CDS
ATTGTCTTGTTTGCCGTTGC+AGG 0.303280 8:-73158610 None:intergenic
GAGAATTTCATTCAGAGGTT+TGG 0.311271 8:-73160111 None:intergenic
AGAATTTCATTCAGAGGTTT+GGG 0.313834 8:-73160110 None:intergenic
ACATGAGTCGTCTTCTTTGA+TGG 0.333051 8:+73159731 MsaT045473.1:CDS
CCTGAATAACCTTTGCTTGT+TGG 0.334230 8:-73159865 None:intergenic
CGCTGATATTCTTTATGAAC+AGG 0.343310 8:+73159479 MsaT045473.1:CDS
GGTTTCCTGGATTCTTTAGA+AGG 0.343832 8:-73159951 None:intergenic
CTTGGCCTACTTTGCTGGTA+AGG 0.345619 8:-73159888 None:intergenic
AGTATTGCTCATTCAGTTCA+TGG 0.346407 8:-73159407 None:intergenic
TTTATTTCTGCAAGAGTCTT+GGG 0.348071 8:-73160023 None:intergenic
GTTCTTGTTCATGATGAATC+TGG 0.352114 8:+73159327 MsaT045473.1:CDS
CTTCTTTGATGGTTCAAAAC+CGG 0.368582 8:+73159742 MsaT045473.1:CDS
ATATTTGCCAGTGCTTGATA+TGG 0.374679 8:+73159380 MsaT045473.1:CDS
GCCATCTCTCACTCAATCTA+TGG 0.380953 8:-73160144 None:intergenic
CTTAGGCTGAGGTTTAATAT+CGG 0.404253 8:-73159082 None:intergenic
TGGGAACAACCTGGGTTATG+AGG 0.406841 8:+73159347 MsaT045473.1:CDS
GGCTTAACCCGAGGGATTGT+TGG 0.407402 8:+73157584 MsaT045473.1:CDS
AAGCTCGTCAGCTGGTTTCC+AGG 0.411618 8:+73160085 MsaT045473.1:CDS
GATCTCTGTTGCTTCACAAT+TGG 0.413086 8:-73159972 None:intergenic
ACCCGAGGGATTGTTGGTAT+TGG 0.421246 8:+73157590 MsaT045473.1:CDS
ATTGTTGGTATTGGTTATCT+GGG 0.423158 8:+73157599 MsaT045473.1:CDS
TTAGAAAATGGAACTGCATC+GGG 0.423544 8:+73158982 MsaT045473.1:CDS
TTATGAGCAACGCCGTCGTC+TGG 0.423938 8:-73159216 None:intergenic
AGTATCTAAGACCATCTCTC+TGG 0.436807 8:-73159574 None:intergenic
CCAGGGGAGGACCGTTCCTC+AGG 0.438642 8:-73159297 None:intergenic
GGAGGAAATGGCCGTTTAGC+TGG 0.441000 8:-73159930 None:intergenic
TTCTTTGATGGTTCAAAACC+GGG 0.442952 8:+73159743 MsaT045473.1:CDS
CTTCCATTGTCATTCTTCAA+AGG 0.447586 8:-73158943 None:intergenic
TGAACTGAATGAGCAATACT+TGG 0.452267 8:+73159410 MsaT045473.1:CDS
CCTGAGGAACGGTCCTCCCC+TGG 0.461925 8:+73159297 MsaT045473.1:CDS
TTCACAATGTGAACTTTCAC+CGG 0.467502 8:-73160063 None:intergenic
TCAGGCCTTACCAGCAAAGT+AGG 0.477593 8:+73159883 MsaT045473.1:CDS
GATTCTTTAGAAGGAGGAAA+TGG 0.479581 8:-73159942 None:intergenic
CATTGTGAAAGCTCGTCAGC+TGG 0.479616 8:+73160077 MsaT045473.1:CDS
ACTGCAAAGCCTTTGAAGAA+TGG 0.483365 8:+73158862 MsaT045473.1:CDS
TTCCTCCTTCTAAAGAATCC+AGG 0.492036 8:+73159946 MsaT045473.1:CDS
TGAACAAGAACATCAAATCC+AGG 0.492763 8:-73159315 None:intergenic
TGAATGAAATTCTCAAAGAC+AGG 0.493790 8:+73160120 MsaT045473.1:CDS
TGATGAATCTGGGAACAACC+TGG 0.493882 8:+73159338 MsaT045473.1:CDS
GCAACGCCGTCGTCTGGTAA+TGG 0.495107 8:-73159210 None:intergenic
CCACAAACTTTCCTTTCACT+AGG 0.496086 8:-73159546 None:intergenic
TTCACCCCTCACTTGCAAGA+AGG 0.501218 8:+73157260 MsaT045473.1:CDS
TGATGGTTCAAAACCGGGAA+AGG 0.501541 8:+73159748 MsaT045473.1:CDS
CCTCCCTGCGTCTTCTCAGA+TGG 0.509631 8:-73159674 None:intergenic
CTTCTTAAGAGACCTGTGTC+AGG 0.510576 8:-73159708 None:intergenic
TTTCCAGTGTCATTCTTCAA+AGG 0.511129 8:-73158751 None:intergenic
CTTGGGCCCTGAAAATGCAT+TGG 0.511654 8:-73160006 None:intergenic
TTTCCAGCGTCATTCTTCAA+AGG 0.516545 8:-73158727 None:intergenic
ACTCCAGTGTGTCCATCCAC+AGG 0.518626 8:-73158559 None:intergenic
ACAGTGAGCGAGCTATCATC+AGG 0.522943 8:-73158700 None:intergenic
TTTGCAGAGTCATTCTTCAA+AGG 0.525080 8:-73158775 None:intergenic
GGTAGCAGAAGATCTTCCTT+AGG 0.532138 8:-73159099 None:intergenic
TTCTTGTTCATGATGAATCT+GGG 0.535477 8:+73159328 MsaT045473.1:CDS
TTCACAGTGTCAATCTTCAA+AGG 0.538247 8:-73158895 None:intergenic
AGCCAAGAAATAAACGCAAT+CGG 0.538661 8:-73157238 None:intergenic
TTCACAGTGTCAATCTTCAA+AGG 0.539488 8:-73158919 None:intergenic
TTTCAATTGCCAACAAGCAA+AGG 0.539740 8:+73159856 MsaT045473.1:CDS
TTGAACCTTCTTGCAAGTGA+GGG 0.541701 8:-73157265 None:intergenic
CTTAGAAAATGGAACTGCAT+CGG 0.543707 8:+73158981 MsaT045473.1:CDS
CCTAGTGAAAGGAAAGTTTG+TGG 0.543844 8:+73159546 MsaT045473.1:CDS
TTTGCAGAGCCATTCTTCAA+AGG 0.546293 8:-73158871 None:intergenic
AGAAGATCTTCCTTAGGCTG+AGG 0.549172 8:-73159093 None:intergenic
CGATATTAAACCTCAGCCTA+AGG 0.549253 8:+73159083 MsaT045473.1:CDS
GAACTCTGCTCAGTGAATGA+AGG 0.549641 8:-73159246 None:intergenic
AACCAATACCAACAATCCCT+CGG 0.549767 8:-73157592 None:intergenic
GCAGGTAATGAAGATTGTGC+AGG 0.551372 8:-73158592 None:intergenic
ACCAATACCAACAATCCCTC+GGG 0.552430 8:-73157591 None:intergenic
TTTGCAGTGTCAATCTTCAA+AGG 0.552856 8:-73158847 None:intergenic
GAACTGAATGAGCAATACTT+GGG 0.554055 8:+73159411 MsaT045473.1:CDS
GGGAACAACCTGGGTTATGA+GGG 0.557547 8:+73159348 MsaT045473.1:CDS
GGTTAAGCCTCGCAATCTCA+TGG 0.558877 8:-73157570 None:intergenic
ATTGAACCTTCTTGCAAGTG+AGG 0.561506 8:-73157266 None:intergenic
AAAACCGGGAAAGGCATCAA+AGG 0.564085 8:+73159757 MsaT045473.1:CDS
ATTCACTGAGCAGAGTTCAG+AGG 0.565256 8:+73159251 MsaT045473.1:CDS
ACAAGCAGCCATCTCAGAAG+TGG 0.566131 8:-73159625 None:intergenic
TCTTTGAGAATTTCATTCAG+AGG 0.567578 8:-73160116 None:intergenic
TCGAATGTCCACTTCTGAGA+TGG 0.567972 8:+73159617 MsaT045473.1:CDS
ATAAAGAAGCTGCTGTGACT+CGG 0.570342 8:+73159178 MsaT045473.1:CDS
GAAGATTGTGCAGGTTCAGA+CGG 0.570354 8:-73158583 None:intergenic
GATTACGACACTATGTGCTC+AGG 0.573085 8:+73159456 MsaT045473.1:CDS
CAGCCTGTGGATGGACACAC+TGG 0.576235 8:+73158556 MsaT045473.1:intron
GTATCTAAGACCATCTCTCT+GGG 0.580465 8:-73159573 None:intergenic
TGGTCATCCATATCAAGCAC+TGG 0.581438 8:-73159387 None:intergenic
TTCCTGGATTCTTTAGAAGG+AGG 0.583182 8:-73159948 None:intergenic
TGGTTTAAATGTGTATCCCT+TGG 0.586917 8:-73159033 None:intergenic
AAATAGTCAAGTAGAGCCTG+AGG 0.587572 8:+73159281 MsaT045473.1:CDS
GAACAAGAACATCAAATCCA+GGG 0.590993 8:-73159314 None:intergenic
CCCGAAAATATTAAACAGCA+AGG 0.591141 8:+73159126 MsaT045473.1:CDS
AGAAGATTAACATCACAAGT+AGG 0.593281 8:+73159804 MsaT045473.1:CDS
GTCAATAGTCCTCCTGACAC+AGG 0.595790 8:+73159696 MsaT045473.1:CDS
ACAATCTTCATTACCTGCAA+CGG 0.598316 8:+73158597 MsaT045473.1:CDS
AAGCCTTTGAAGAATGACAC+TGG 0.602847 8:+73158748 MsaT045473.1:CDS
TTGTGGTTATCCCAGAGAGA+TGG 0.602992 8:+73159563 MsaT045473.1:CDS
TGAACCTTCTTGCAAGTGAG+GGG 0.613141 8:-73157264 None:intergenic
GATGAATCTGGGAACAACCT+GGG 0.613350 8:+73159339 MsaT045473.1:CDS
AGGGTGTAGAATGTGAGTAT+AGG 0.616264 8:+73157545 MsaT045473.1:intron
AGGCATCAAAGGCAAGACAT+TGG 0.618149 8:+73159768 MsaT045473.1:CDS
AACAACCTGGGTTATGAGGG+CGG 0.623566 8:+73159351 MsaT045473.1:CDS
TGTAGCATCGTCCACACCAA+GGG 0.625248 8:+73159017 MsaT045473.1:CDS
GATTGCGAGGCTTAACCCGA+GGG 0.627528 8:+73157576 MsaT045473.1:CDS
GGGTGTCTAAAAGCACAAGT+TGG 0.629066 8:-73157634 None:intergenic
AAGCCTTTGAAGAATGACGC+TGG 0.631477 8:+73158724 MsaT045473.1:CDS
TAAATGTGTATCCCTTGGTG+TGG 0.636452 8:-73159028 None:intergenic
GTCACCATCTGAGAAGACGC+AGG 0.644495 8:+73159670 MsaT045473.1:CDS
GTCAAGTAGAGCCTGAGGAA+CGG 0.645293 8:+73159286 MsaT045473.1:CDS
AAGCCTTTGAAGAATGACAA+TGG 0.648926 8:+73158940 MsaT045473.1:CDS
GTGTAGCATCGTCCACACCA+AGG 0.650640 8:+73159016 MsaT045473.1:CDS
TCACCATCTGAGAAGACGCA+GGG 0.650947 8:+73159671 MsaT045473.1:CDS
CTTAAGAGACCTGTGTCAGG+AGG 0.654168 8:-73159705 None:intergenic
ATAGGCACCATGAGATTGCG+AGG 0.657532 8:+73157563 MsaT045473.1:CDS
AGATTGCGAGGCTTAACCCG+AGG 0.660519 8:+73157575 MsaT045473.1:CDS
CAGAACCGCCCTCATAACCC+AGG 0.660881 8:-73159356 None:intergenic
AACAAGAACATCAAATCCAG+GGG 0.675043 8:-73159313 None:intergenic
AAGAACATCAAATCCAGGGG+AGG 0.679312 8:-73159310 None:intergenic
AACTGAATGAGCAATACTTG+GGG 0.681764 8:+73159412 MsaT045473.1:CDS
TGCAACGGCAAACAAGACAA+TGG 0.690022 8:+73158612 MsaT045473.1:CDS
AAAGGCAAGACATTGGTCAG+CGG 0.696430 8:+73159775 MsaT045473.1:CDS
GACTCTCCATTACCAGACGA+CGG 0.716277 8:+73159204 MsaT045473.1:CDS
CCATCTGAGAAGACGCAGGG+AGG 0.719477 8:+73159674 MsaT045473.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 73157210 73160154 73157210 ID=MsaG045473
Chr8 mRNA 73157210 73160154 73157210 ID=MsaT045473.1;Parent=MsaG045473
Chr8 exon 73157210 73157281 73157210 ID=MsaT045473.1.exon1;Parent=MsaT045473.1
Chr8 CDS 73157210 73157281 73157210 ID=cds.MsaT045473.1;Parent=MsaT045473.1
Chr8 exon 73157547 73157657 73157547 ID=MsaT045473.1.exon2;Parent=MsaT045473.1
Chr8 CDS 73157547 73157657 73157547 ID=cds.MsaT045473.1;Parent=MsaT045473.1
Chr8 exon 73158559 73160154 73158559 ID=MsaT045473.1.exon3;Parent=MsaT045473.1
Chr8 CDS 73158559 73160154 73158559 ID=cds.MsaT045473.1;Parent=MsaT045473.1
Gene Sequence

>MsaT045473.1

ATGGACGAAGATTTTCAAAAGCGTAACACCGATTGCGTTTATTTCTTGGCTTCACCCCTCACTTGCAAGAAGGGTGTAGAATGTGAGTATAGGCACCATGAGATTGCGAGGCTTAACCCGAGGGATTGTTGGTATTGGTTATCTGGGAATTGTCTTAATCCAACTTGTGCTTTTAGACACCCTCCTGTGGATGGACACACTGGAGTGCCGTCTGAACCTGCACAATCTTCATTACCTGCAACGGCAAACAAGACAATGGTTCCCTGTTACTTTTTCTTCAATGGGTTTTGCAACAAAGGTAGCAAATGCTCATTTTTGCATGGACCTGATGATAGCTCGCTCACTGTAAAGCCTTTGAAGAATGACGCTGGAAAGCCTTTGAAGAATGACACTGGAAAGCCTTTGAAGAATGACTCTGCAAAGCCTTTAAAGAATGACTCTGCAAAGCCTTTAAAGAATGACTCTGCAAAACCTTTGAAGATTGACACTGCAAAGCCTTTGAAGAATGGCTCTGCAAAACCTTTGAAGATTGACACTGTGAAGCCTTTGAAGATTGACACTGTGAAGCCTTTGAAGAATGACAATGGAAGCATTAATAAACCCAACTTAGAAAATGGAACTGCATCGGGAAATAAAACAAGTGTAGCATCGTCCACACCAAGGGATACACATTTAAACCAATCTTTATCTTCTCCAAAAGCTTTGTCCGATATTAAACCTCAGCCTAAGGAAGATCTTCTGCTACCATTACCCGAAAATATTAAACAGCAAGGTGATTGCTTCAAAATTTCTTCTTTGGACTATAAAGAAGCTGCTGTGACTCGGTCAGACTCTCCATTACCAGACGACGGCGTTGCTCATAACATGTCTCCTTCATTCACTGAGCAGAGTTCAGAGGAGCAAGAAAATAGTCAAGTAGAGCCTGAGGAACGGTCCTCCCCTGGATTTGATGTTCTTGTTCATGATGAATCTGGGAACAACCTGGGTTATGAGGGCGGTTCTGAATATTTGCCAGTGCTTGATATGGATGACCATGAACTGAATGAGCAATACTTGGGGTATGAATTTAAAAACACAAATGATTACGACACTATGTGCTCAGGCGCTGATATTCTTTATGAACAGGAAACATATGATGATTACAGATGTTTTGATAGGGATTTTACTAGTCCTAGTGAAAGGAAAGTTTGTGGTTATCCCAGAGAGATGGTCTTAGATACTATATTTTCCAGGAAAAGGATTCGAATGTCCACTTCTGAGATGGCTGCTTGTGACTACTCTGACTTAGATCTACGTCACCATCTGAGAAGACGCAGGGAGGTCAATAGTCCTCCTGACACAGGTCTCTTAAGAAGACATGAGTCGTCTTCTTTGATGGTTCAAAACCGGGAAAGGCATCAAAGGCAAGACATTGGTCAGCGGCAAAGTAGAAGATTAACATCACAAGTAGGATTTAGTTCAATCAGAGAAGTTGAAGATCTTTCAATTGCCAACAAGCAAAGGTTATTCAGGCCTTACCAGCAAAGTAGGCCAAGAAAGCACTATAAAGAAAAACCAGCTAAACGGCCATTTCCTCCTTCTAAAGAATCCAGGAAACCAATTGTGAAGCAACAGAGATCTATTCAGAAATCCAATGCATTTTCAGGGCCCAAGACTCTTGCAGAAATAAAAGAAGAGAAGAAAAAGTCCGGTGAAAGTTCACATTGTGAAAGCTCGTCAGCTGGTTTCCAGGATCCCAAACCTCTGAATGAAATTCTCAAAGACAGGACCATAGATTGA
Protein sequence

>MsaT045473.1

MDEDFQKRNTDCVYFLASPLTCKKGVECEYRHHEIARLNPRDCWYWLSGNCLNPTCAFRHPPVDGHTGVPSEPAQSSLPATANKTMVPCYFFFNGFCNKGSKCSFLHGPDDSSLTVKPLKNDAGKPLKNDTGKPLKNDSAKPLKNDSAKPLKNDSAKPLKIDTAKPLKNGSAKPLKIDTVKPLKIDTVKPLKNDNGSINKPNLENGTASGNKTSVASSTPRDTHLNQSLSSPKALSDIKPQPKEDLLLPLPENIKQQGDCFKISSLDYKEAAVTRSDSPLPDDGVAHNMSPSFTEQSSEEQENSQVEPEERSSPGFDVLVHDESGNNLGYEGGSEYLPVLDMDDHELNEQYLGYEFKNTNDYDTMCSGADILYEQETYDDYRCFDRDFTSPSERKVCGYPREMVLDTIFSRKRIRMSTSEMAACDYSDLDLRHHLRRRREVNSPPDTGLLRRHESSSLMVQNRERHQRQDIGQRQSRRLTSQVGFSSIREVEDLSIANKQRLFRPYQQSRPRKHYKEKPAKRPFPPSKESRKPIVKQQRSIQKSNAFSGPKTLAEIKEEKKKSGESSHCESSSAGFQDPKPLNEILKDRTID*