AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045507

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045507 MtrunA17_Chr4g0063931 95.288 191 7 1 1 189 1 191 8.82e-136 377
MsaG045507 MtrunA17_Chr2g0322221 56.667 180 67 4 5 173 2 181 8.86e-61 187
MsaG045507 MtrunA17_Chr4g0011841 53.403 191 63 4 14 184 25 209 9.00e-55 173
MsaG045507 MtrunA17_Chr4g0025361 56.647 173 61 5 5 173 2 164 1.18e-53 169
MsaG045507 MtrunA17_Chr4g0056521 53.757 173 58 4 8 163 14 181 4.62e-50 161
MsaG045507 MtrunA17_Chr5g0413381 49.686 159 58 4 12 156 12 162 8.02e-46 149
MsaG045507 MtrunA17_Chr5g0419871 43.125 160 70 3 5 155 8 155 4.20e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045507 AT2G26580.2 75.301 166 35 2 7 172 4 163 2.17e-87 254
MsaG045507 AT2G26580.1 75.301 166 35 2 7 172 4 163 2.17e-87 254
MsaG045507 AT1G08465.1 54.190 179 59 5 5 171 2 169 4.53e-56 175
MsaG045507 AT2G45190.1 54.802 177 63 6 4 163 14 190 1.26e-54 173
MsaG045507 AT4G00180.1 50.549 182 62 5 12 165 23 204 2.95e-48 157
MsaG045507 AT4G00180.2 48.555 173 61 5 21 165 1 173 5.15e-40 135
MsaG045507 AT1G23420.1 47.205 161 71 3 13 159 20 180 7.27e-39 133
MsaG045507 AT1G23420.2 47.205 161 71 3 13 159 51 211 1.99e-38 133
MsaG045507 AT2G45190.2 92.982 57 4 0 107 163 70 126 3.77e-34 119
MsaG045507 AT1G69180.1 42.069 145 78 2 11 155 18 156 6.09e-32 114

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATCCTATCGTTGCATT+TGG 0.296610 8:+73596753 None:intergenic
GCTTCTCTGTGACTGATATC+AGG 0.363234 8:+73596243 None:intergenic
GCAATCCTATCGTTGCATTT+GGG 0.399881 8:+73596754 None:intergenic
ACTGTCATGGCAAGATGTTC+AGG 0.423195 8:-73597830 MsaT045507.1:intron
GTGGTCACTGCACAAATCTA+TGG 0.424333 8:-73597882 MsaT045507.1:CDS
AGGTACACGTTGCCTCTTCT+CGG 0.458670 8:+73596432 None:intergenic
ATCAGCCCAAAATGAATATG+AGG 0.476928 8:+73596102 None:intergenic
CACCCATGTTACTGAGGAAA+GGG 0.483814 8:-73596713 MsaT045507.1:CDS
GAGCAACAATCAAGCTAAGA+TGG 0.499438 8:-73596073 MsaT045507.1:CDS
TCCTCAGTAACATGGGTGGT+TGG 0.501150 8:+73596718 None:intergenic
GGTACACGTTGCCTCTTCTC+GGG 0.503039 8:+73596433 None:intergenic
CAACCCTTTCCTCAGTAACA+TGG 0.504380 8:+73596710 None:intergenic
TGAGAAGCGTTTGATGTCTA+CGG 0.515736 8:-73595942 MsaT045507.1:CDS
ATGTAGCAGAGTTGTTCAGC+AGG 0.519727 8:+73598977 None:intergenic
TATACTCTGGATTCATGCAC+TGG 0.523585 8:+73596797 None:intergenic
AGTGCATGAATCCAGAGTAT+AGG 0.525878 8:-73596795 MsaT045507.1:CDS
CCTATCGTTGCATTTGGGAG+TGG 0.533061 8:+73596759 None:intergenic
ACAATATTGCAAAAGTTGCA+AGG 0.540036 8:+73598953 None:intergenic
AATTTCCAGCTCCCGAGAAG+AGG 0.540719 8:-73596444 MsaT045507.1:intron
AAATCTATGGTCTGTAAACA+TGG 0.552321 8:-73597869 MsaT045507.1:CDS
CCGCGTTTCAGTCACTGTCA+TGG 0.556178 8:-73597843 MsaT045507.1:CDS
TCTGGATTCATGCACTGGCT+AGG 0.560862 8:+73596802 None:intergenic
CTAGTTCGAATCCTATACTC+TGG 0.567724 8:+73596784 None:intergenic
GGAAGAGATTCAGAGGATCA+AGG 0.576711 8:-73596274 MsaT045507.1:CDS
CAATATTGCAAAAGTTGCAA+GGG 0.579397 8:+73598954 None:intergenic
CCACCCATGTTACTGAGGAA+AGG 0.581614 8:-73596714 MsaT045507.1:CDS
ATGAACTGATTATAAGCAGA+AGG 0.586762 8:+73596412 None:intergenic
CCACTCCCAAATGCAACGAT+AGG 0.592244 8:-73596759 MsaT045507.1:CDS
AGACATGTCTAGCTGTAGCA+TGG 0.594915 8:-73599008 None:intergenic
ATTTCAAACAAGCTACTGCA+TGG 0.595371 8:+73597919 None:intergenic
TATGCAGGGAAGAGATTCAG+AGG 0.621557 8:-73596281 MsaT045507.1:intron
AACCCTTTCCTCAGTAACAT+GGG 0.626838 8:+73596711 None:intergenic
TGTGCAGTGACCACATCGAA+CGG 0.632060 8:+73597891 None:intergenic
ACCAACCACCCATGTTACTG+AGG 0.669098 8:-73596719 MsaT045507.1:CDS
GAAATAGTGACCGTTCGATG+TGG 0.670282 8:-73597901 MsaT045507.1:CDS
CCTTTCCTCAGTAACATGGG+TGG 0.674444 8:+73596714 None:intergenic
CCATGACAGTGACTGAAACG+CGG 0.691630 8:+73597843 None:intergenic
TGACAGTGACTGAAACGCGG+CGG 0.753574 8:+73597846 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 73595922 73599026 73595922 ID=MsaG045507
Chr8 mRNA 73595922 73599026 73595922 ID=MsaT045507.1;Parent=MsaG045507
Chr8 exon 73595922 73595969 73595922 ID=MsaT045507.1.exon7;Parent=MsaT045507.1
Chr8 CDS 73595922 73595969 73595922 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Chr8 exon 73596068 73596136 73596068 ID=MsaT045507.1.exon6;Parent=MsaT045507.1
Chr8 CDS 73596068 73596136 73596068 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Chr8 exon 73596221 73596296 73596221 ID=MsaT045507.1.exon5;Parent=MsaT045507.1
Chr8 CDS 73596221 73596296 73596221 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Chr8 exon 73596409 73596457 73596409 ID=MsaT045507.1.exon4;Parent=MsaT045507.1
Chr8 CDS 73596409 73596457 73596409 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Chr8 exon 73596701 73596827 73596701 ID=MsaT045507.1.exon3;Parent=MsaT045507.1
Chr8 CDS 73596701 73596827 73596701 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Chr8 exon 73597831 73597950 73597831 ID=MsaT045507.1.exon2;Parent=MsaT045507.1
Chr8 CDS 73597831 73597950 73597831 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Chr8 exon 73598946 73599026 73598946 ID=MsaT045507.1.exon1;Parent=MsaT045507.1
Chr8 CDS 73598946 73599026 73598946 ID=cds.MsaT045507.1;Parent=MsaT045507.1
Gene Sequence

>MsaT045507.1

ATGTCTAGCTGTAGCATGGATGTTGCACCTGCTGAACAACTCTGCTACATCCCTTGCAACTTTTGCAATATTGTTCTTGCGGTGAGTGTTCCATGCAGTAGCTTGTTTGAAATAGTGACCGTTCGATGTGGTCACTGCACAAATCTATGGTCTGTAAACATGGCCGCCGCGTTTCAGTCACTGTCATGGCAAGATGTTCAGGCACCTAGCCAGTGCATGAATCCAGAGTATAGGATTCGAACTAGCTCCACTCCCAAATGCAACGATAGGATTGCAATGCGTTCTGCACCAACCACCCATGTTACTGAGGAAAGGGTTGTTAACCGCCCTCCCGAGAAGAGGCAACGTGTACCTTCTGCTTATAATCAGTTCATCAAGGAAGAGATTCAGAGGATCAAGGCTAATAATCCTGATATCAGTCACAGAGAAGCTTTTAGTACAGCTGCAAAGAACTGGGCACATTTTCCTCATATTCATTTTGGGCTGATGTTGGAGAGCAACAATCAAGCTAAGATGGAGACTGTCTCTGAGAAGCGTTTGATGTCTACGGCTGCGCTAATGAACAAATGA
Protein sequence

>MsaT045507.1

MSSCSMDVAPAEQLCYIPCNFCNIVLAVSVPCSSLFEIVTVRCGHCTNLWSVNMAAAFQSLSWQDVQAPSQCMNPEYRIRTSSTPKCNDRIAMRSAPTTHVTEERVVNRPPEKRQRVPSAYNQFIKEEIQRIKANNPDISHREAFSTAAKNWAHFPHIHFGLMLESNNQAKMETVSEKRLMSTAALMNK*