AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045512

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045512 MtrunA17_Chr4g0064011 78.571 238 42 3 1 235 1 232 2.10e-121 344
MsaG045512 MtrunA17_Chr3g0122071 46.748 246 93 5 1 232 1 222 1.66e-56 179
MsaG045512 MtrunA17_Chr6g0474091 62.903 124 40 3 1 121 1 121 1.26e-36 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045512 AT2G18490.1 63.158 57 21 0 123 179 155 211 1.03e-20 87.8
MsaG045512 AT2G18490.2 63.158 57 21 0 123 179 205 261 1.71e-20 88.2
MsaG045512 AT5G22990.1 65.079 63 21 1 118 179 227 289 5.56e-20 87.0
MsaG045512 AT2G15740.1 59.701 67 23 1 122 188 229 291 6.25e-20 87.0
MsaG045512 AT5G42640.1 42.478 113 47 4 79 179 179 285 1.24e-19 85.9

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCATTGAGCTTTAGAATC+TGG 0.282776 8:+73692206 None:intergenic
GTTTATTGTGGGGTAGACTA+TGG 0.293623 8:+73692374 None:intergenic
AACAATATTGTTGCTACTAA+TGG 0.346446 8:-73692469 MsaT045512.1:CDS
CAAATGCTTAAAACACAGTT+TGG 0.350424 8:-73692643 MsaT045512.1:CDS
TACTAATGGTGATGAAGTTC+GGG 0.365107 8:-73692455 MsaT045512.1:CDS
CTACTAATGGTGATGAAGTT+CGG 0.369876 8:-73692456 MsaT045512.1:CDS
TCTGGAGATCTGGTCTCTTT+CGG 0.392995 8:+73692224 None:intergenic
AGCTTTAGAATCTGGAGATC+TGG 0.424493 8:+73692214 None:intergenic
TCAGCCGATAGTAACAATTT+CGG 0.440639 8:-73692697 MsaT045512.1:CDS
TGTATTGATTTGATGATGCC+TGG 0.446641 8:+73692664 None:intergenic
GCTATTACCTTGTTGCACTT+TGG 0.453373 8:+73692334 None:intergenic
TTGCACTTTGGACATATGTA+TGG 0.455080 8:+73692346 None:intergenic
TTATTATTATTATCAGATGA+TGG 0.455461 8:+73692601 None:intergenic
GACGAGCCTCCAAGGAGATC+AGG 0.458143 8:+73692514 None:intergenic
TCCCTGCTCCTCCTCTAGGT+GGG 0.461859 8:-73692099 MsaT045512.1:CDS
AGTGTCCCTGCTCCTCCTCT+AGG 0.465611 8:-73692103 MsaT045512.1:CDS
CAACCACCATGAGTTCTGAT+AGG 0.471836 8:-73692759 None:intergenic
ACAATATTGTTTGCCTCAGA+TGG 0.473153 8:+73692481 None:intergenic
TACGCTCATCCTGATCTCCT+TGG 0.478788 8:-73692523 MsaT045512.1:CDS
ACCCCACCTAGAGGAGGAGC+AGG 0.494555 8:+73692097 None:intergenic
AAAGAAGAAATTGTTGATGA+TGG 0.494936 8:-73692403 MsaT045512.1:CDS
TGTATGGTCCATGTTTATTG+TGG 0.503637 8:+73692362 None:intergenic
GTTCTGATAGGTTGATGAAA+TGG 0.508320 8:-73692747 MsaT045512.1:CDS
TATTGTTTGCCTCAGATGGC+GGG 0.510054 8:+73692485 None:intergenic
CCCTGCTCCTCCTCTAGGTG+GGG 0.517318 8:-73692098 MsaT045512.1:CDS
CAGATTCTAAAGCTCAATGA+TGG 0.517574 8:-73692205 MsaT045512.1:CDS
GTCCCTGCTCCTCCTCTAGG+TGG 0.522388 8:-73692100 MsaT045512.1:CDS
AATATATATTAATTATCAGC+AGG 0.528844 8:+73692058 None:intergenic
GTATGGTCCATGTTTATTGT+GGG 0.530171 8:+73692363 None:intergenic
GCTCATCCTGATCTCCTTGG+AGG 0.534371 8:-73692520 MsaT045512.1:CDS
CATCAACCTATCAGAACTCA+TGG 0.543143 8:+73692753 None:intergenic
CAAATAGTACCAACATGAAG+AGG 0.557023 8:-73692429 MsaT045512.1:CDS
GGTGTGATGACGAGCCTCCA+AGG 0.564529 8:+73692506 None:intergenic
AATTTCGGTAGTCATGCTCC+AGG 0.573474 8:-73692682 MsaT045512.1:CDS
ACTACCGAAATTGTTACTAT+CGG 0.574375 8:+73692693 None:intergenic
ATATTGTTTGCCTCAGATGG+CGG 0.593923 8:+73692484 None:intergenic
TGAGTATCATAACTGACAGA+AGG 0.596005 8:+73692133 None:intergenic
GTCATCACACCCGCCATCTG+AGG 0.607113 8:-73692494 MsaT045512.1:CDS
GTTACTATCGGCTGATGATG+TGG 0.609878 8:+73692705 None:intergenic
TATGGTCCATGTTTATTGTG+GGG 0.620016 8:+73692364 None:intergenic
CATATGTCCAAAGTGCAACA+AGG 0.620267 8:-73692341 MsaT045512.1:CDS
TGTTATCAACAACAACAAGA+TGG 0.620498 8:-73692566 MsaT045512.1:CDS
TGATTAGTAGAAGCAATGAC+AGG 0.628764 8:+73692166 None:intergenic
TGTATCAAGTCACTACAAGT+CGG 0.647352 8:-73692287 MsaT045512.1:CDS
AGTCTACCCCACAATAAACA+TGG 0.648429 8:-73692370 MsaT045512.1:CDS
CCCCACCTAGAGGAGGAGCA+GGG 0.690886 8:+73692098 None:intergenic
AGGTTGATGAAATGGTTGTG+TGG 0.707031 8:-73692739 MsaT045512.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 73692066 73692773 73692066 ID=MsaG045512
Chr8 mRNA 73692066 73692773 73692066 ID=MsaT045512.1;Parent=MsaG045512
Chr8 exon 73692066 73692773 73692066 ID=MsaT045512.1.exon1;Parent=MsaT045512.1
Chr8 CDS 73692066 73692773 73692066 ID=cds.MsaT045512.1;Parent=MsaT045512.1
Gene Sequence

>MsaT045512.1

ATGAGTTCTGATAGGTTGATGAAATGGTTGTGTGGCTACAACACAACCACATCATCAGCCGATAGTAACAATTTCGGTAGTCATGCTCCAGGCATCATCAAATCAATACAAATGCTTAAAACACAGTTTGGAAATCTACAAGCATCATCACCATCATCTGATAATAATAATAATAATAGGAATGATGTTATCAACAACAACAAGATGGTGTTGAACACTTCTTCTGCTTACGCTCATCCTGATCTCCTTGGAGGCTCGTCATCACACCCGCCATCTGAGGCAAACAATATTGTTGCTACTAATGGTGATGAAGTTCGGGCTTCAAATAGTACCAACATGAAGAGGAAAAAAGAAGAAATTGTTGATGATGGTAGAATCCATAGTCTACCCCACAATAAACATGGACCATACATATGTCCAAAGTGCAACAAGGTAATAGCAACATCACAAAAATTCGCTGCTCATGTATCAAGTCACTACAAGTCGGAAAGTGAAGAAGAAAGAAAAAAGAGATACATGTCTAGGATCCGAAAGAGACCAGATCTCCAGATTCTAAAGCTCAATGATGGAACAACTACTCTTGTTCCTGTCATTGCTTCTACTAATCAGTCACATGCTCCTTCTGTCAGTTATGATACTCAAAATATGAGTGTCCCTGCTCCTCCTCTAGGTGGGGTAAAAGTTAAACATGAGCCTGCTGATAATTAA
Protein sequence

>MsaT045512.1

MSSDRLMKWLCGYNTTTSSADSNNFGSHAPGIIKSIQMLKTQFGNLQASSPSSDNNNNNRNDVINNNKMVLNTSSAYAHPDLLGGSSSHPPSEANNIVATNGDEVRASNSTNMKRKKEEIVDDGRIHSLPHNKHGPYICPKCNKVIATSQKFAAHVSSHYKSESEEERKKRYMSRIRKRPDLQILKLNDGTTTLVPVIASTNQSHAPSVSYDTQNMSVPAPPLGGVKVKHEPADN*