AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045614

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045614 MtrunA17_Chr4g0065241 95.906 342 3 2 1 332 1 341 0.0 672
MsaG045614 MtrunA17_Chr2g0305101 71.622 370 63 10 1 332 1 366 1.43e-178 498
MsaG045614 MtrunA17_Chr1g0149651 76.493 268 49 4 67 321 169 435 8.43e-147 421
MsaG045614 MtrunA17_Chr3g0137241 75.000 268 49 4 70 321 105 370 4.46e-142 406
MsaG045614 MtrunA17_Chr5g0398181 65.799 269 78 3 67 321 23 291 3.98e-122 353
MsaG045614 MtrunA17_Chr1g0192791 33.473 239 148 5 69 306 17 245 2.48e-27 108
MsaG045614 MtrunA17_Chr2g0289201 31.933 238 141 6 72 306 118 337 5.05e-26 106
MsaG045614 MtrunA17_Chr1g0151961 32.794 247 147 6 62 306 113 342 6.11e-25 103
MsaG045614 MtrunA17_Chr5g0413201 32.500 240 139 7 72 306 73 294 1.32e-23 99.0
MsaG045614 MtrunA17_Chr5g0439391 29.339 242 135 8 74 306 49 263 4.30e-19 86.3
MsaG045614 MtrunA17_Chr4g0028701 44.118 68 36 1 239 306 464 529 8.69e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045614 AT5G11060.1 77.007 274 43 5 71 329 123 391 3.44e-147 420
MsaG045614 AT5G25220.1 76.277 274 45 4 71 329 159 427 4.06e-147 421
MsaG045614 AT5G25220.2 76.981 265 42 3 71 321 159 418 3.76e-145 416
MsaG045614 AT4G32040.1 70.260 269 64 6 71 326 118 383 2.64e-127 369
MsaG045614 AT4G32040.2 70.833 264 61 6 71 321 118 378 3.13e-126 367
MsaG045614 AT1G62990.1 61.733 277 89 4 59 321 17 290 1.91e-119 346
MsaG045614 AT1G23380.1 33.217 286 141 11 33 306 58 305 1.57e-28 113
MsaG045614 AT4G08150.1 34.553 246 130 7 72 306 135 360 8.44e-28 112
MsaG045614 AT1G70510.1 31.752 274 147 10 68 332 66 308 1.35e-27 110
MsaG045614 AT1G23380.2 32.986 288 141 12 33 306 58 307 9.49e-27 108
MsaG045614 AT1G70510.2 30.657 274 154 9 68 332 66 312 3.15e-26 106
MsaG045614 AT1G62360.1 33.473 239 138 6 72 306 122 343 1.08e-22 97.8

Find 79 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGAGCTTGATCACTTTA+TGG 0.159839 8:-75073789 MsaT045614.1:intron
TTCTGTTGGAATTGGAAATA+TGG 0.205132 8:-75073816 MsaT045614.1:CDS
CTACCTCTTGCGCTTGGTTT+TGG 0.213600 8:+75068154 None:intergenic
AAATATTCTTCTGTTGGAAT+TGG 0.277819 8:-75073824 MsaT045614.1:CDS
GAATACAAAGCAGAGATATT+AGG 0.306187 8:-75073965 MsaT045614.1:CDS
AATTAAAGCAAATAAATAAC+TGG 0.339856 8:-75068232 MsaT045614.1:CDS
GGTGGCAATCACATTCTAAA+TGG 0.351052 8:-75068412 MsaT045614.1:CDS
TTTCGCAATTGTTGTCATTC+CGG 0.366302 8:+75074002 None:intergenic
GCTAGATTGGTGCAGGAAAC+AGG 0.372105 8:-75068260 MsaT045614.1:CDS
GGTCTTTGATGGAACGAGTT+AGG 0.372149 8:-75068559 MsaT045614.1:CDS
AACGAAGGAAGCAGCCTTGA+TGG 0.386285 8:-75068629 MsaT045614.1:CDS
TCAACAGAATTTCATGCAAA+TGG 0.400557 8:-75074062 MsaT045614.1:CDS
AGAGACTGAAAGGTCTTTGA+TGG 0.405462 8:-75068570 MsaT045614.1:CDS
GGCGTCGATACTCTTGGCTT+TGG 0.416116 8:-75068608 MsaT045614.1:CDS
TGGAGGCAGTGATGGCTTGT+TGG 0.423089 8:-75070493 MsaT045614.1:CDS
GCTGGAAAGCTATTTCTTGT+TGG 0.428428 8:+75074152 None:intergenic
GGAGGCAGTGATGGCTTGTT+GGG 0.429166 8:-75070492 MsaT045614.1:CDS
ATTATGAGCAGGTGTATCTT+CGG 0.435057 8:-75068720 MsaT045614.1:intron
ATTCTACGAAAGAGAAGAGC+TGG 0.436569 8:-75068473 MsaT045614.1:CDS
CTTGATGGCGTCGATACTCT+TGG 0.437382 8:-75068614 MsaT045614.1:CDS
CAGTCTCTGCAAAGCTTGAC+AGG 0.437852 8:-75070461 MsaT045614.1:intron
CATAAAGTGATCAAGCTCCT+TGG 0.444189 8:+75073790 None:intergenic
ATCCTTGGAAGCTGATCAAC+CGG 0.446297 8:+75073884 None:intergenic
ATTGCCACCAAGCTTTCAAA+AGG 0.450033 8:+75068426 None:intergenic
ATGATATCTTACAGTTGGAT+AGG 0.451403 8:+75068388 None:intergenic
ACTGCTGTTGTTGTTGTGCC+AGG 0.456266 8:+75074096 None:intergenic
GGAGGAAGATAAGGCTAGAT+TGG 0.457401 8:-75068273 MsaT045614.1:CDS
AGAAGAAGAAGATGAAGAAA+GGG 0.460074 8:-75074122 MsaT045614.1:CDS
TTGTTGCAACTGTTCTTTGA+AGG 0.460514 8:+75070535 None:intergenic
GATTGATGCTCAGCTTCAAC+AGG 0.462130 8:-75073864 MsaT045614.1:CDS
CAGAAGAAGAAGATGAAGAA+AGG 0.465111 8:-75074123 MsaT045614.1:CDS
TCAATCAAAGGAAAAGGAAC+TGG 0.471396 8:-75068205 MsaT045614.1:CDS
AGAAGAGCTGGGAAACTCCC+TGG 0.471897 8:-75068461 MsaT045614.1:CDS
GTATCTTCGGGTGAAGGAAC+AGG 0.474653 8:-75068707 MsaT045614.1:CDS
ATAAAGTGATCAAGCTCCTT+GGG 0.475708 8:+75073791 None:intergenic
AAAAGGGATGTGGTGTCCCC+AGG 0.478534 8:+75068443 None:intergenic
TTATGAGCAGGTGTATCTTC+GGG 0.502400 8:-75068719 MsaT045614.1:intron
GTCAGACGATGAAGATGAGC+AGG 0.503765 8:-75068675 MsaT045614.1:CDS
TCAGTCTCTGTGGGGACTAG+AGG 0.508899 8:+75068584 None:intergenic
CACCGGTTGATCAGCTTCCA+AGG 0.508909 8:-75073886 MsaT045614.1:CDS
AAGATGAAGAAAGGGCATCC+TGG 0.510979 8:-75074114 MsaT045614.1:CDS
GAAGAGCTGGGAAACTCCCT+GGG 0.514675 8:-75068460 MsaT045614.1:CDS
TGAAGCTGAGCATCAATCCT+TGG 0.515964 8:+75073869 None:intergenic
ATGGTACTACCTCTTGCGCT+TGG 0.518902 8:+75068148 None:intergenic
GGTTTATCAATCAAAGGAAA+AGG 0.523630 8:-75068211 MsaT045614.1:CDS
TCATGCCATGGAGGCAGTGA+TGG 0.523998 8:-75070501 MsaT045614.1:CDS
GCTCCAAAACCAAGCGCAAG+AGG 0.527063 8:-75068157 MsaT045614.1:intron
TGATATCTTACAGTTGGATA+GGG 0.527756 8:+75068389 None:intergenic
CTTCTTCTTCTGTGAAACGC+TGG 0.532142 8:+75074134 None:intergenic
AGATAAGGCTAGATTGGTGC+AGG 0.536981 8:-75068267 MsaT045614.1:CDS
CGCAATTGTTGTCATTCCGG+TGG 0.538196 8:+75074005 None:intergenic
TTTGGAGCTACTAGAGGATG+GGG 0.544084 8:+75068172 None:intergenic
AAAGACCTTTCAGTCTCTGT+GGG 0.544199 8:+75068575 None:intergenic
AAAGCATGGATCGGAACCAC+CGG 0.544529 8:-75074021 MsaT045614.1:CDS
GTGTCTCAGAATTGCGACAC+CGG 0.547847 8:-75073903 MsaT045614.1:CDS
ACAAGCCATCACTGCCTCCA+TGG 0.554613 8:+75070496 None:intergenic
GCAGGTGTATCTTCGGGTGA+AGG 0.559026 8:-75068713 MsaT045614.1:intron
ATAACTGGTTTATCAATCAA+AGG 0.563797 8:-75068217 MsaT045614.1:CDS
CCAAGCTTTCAAAAGGGATG+TGG 0.573990 8:+75068433 None:intergenic
AAAGGGATGTGGTGTCCCCA+GGG 0.576707 8:+75068444 None:intergenic
TTCTACGAAAGAGAAGAGCT+GGG 0.579028 8:-75068472 MsaT045614.1:CDS
ACATGTCCGTGTTCATGCCA+TGG 0.585181 8:-75070513 MsaT045614.1:CDS
TCATGCAAATGGAACCAGAA+AGG 0.585338 8:-75074051 MsaT045614.1:CDS
TAGTCCCCACAGAGACTGAA+AGG 0.587654 8:-75068580 MsaT045614.1:CDS
GCAAATGGAACCAGAAAGGA+GGG 0.590244 8:-75074047 MsaT045614.1:CDS
GAATGACAACAATTGCGAAA+CGG 0.591352 8:-75073999 MsaT045614.1:CDS
CAAAGACCTTTCAGTCTCTG+TGG 0.597386 8:+75068574 None:intergenic
GATAATAATTGATCATAGAG+TGG 0.604741 8:+75073938 None:intergenic
TGCAAATGGAACCAGAAAGG+AGG 0.604949 8:-75074048 MsaT045614.1:CDS
CAAGAGTATCGACGCCATCA+AGG 0.607066 8:+75068615 None:intergenic
TTGCCACCAAGCTTTCAAAA+GGG 0.608799 8:+75068427 None:intergenic
AATTGGAAATATGGATCCCA+AGG 0.614560 8:-75073807 MsaT045614.1:CDS
CTGCCTCCATGGCATGAACA+CGG 0.617572 8:+75070507 None:intergenic
AACGCTAGGTTATACAACGA+AGG 0.654984 8:-75068644 MsaT045614.1:CDS
TTGAAGCATGAACTCAAACA+AGG 0.656993 8:-75068530 MsaT045614.1:intron
AGCAGGCAGAGAGTAACGCT+AGG 0.662764 8:-75068658 MsaT045614.1:CDS
TGTCCGTGTTCATGCCATGG+AGG 0.690107 8:-75070510 MsaT045614.1:CDS
AAGACCTTTCAGTCTCTGTG+GGG 0.730543 8:+75068576 None:intergenic
AAGAGCTGGGAAACTCCCTG+GGG 0.766388 8:-75068459 MsaT045614.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 75067959 75074194 75067959 ID=MsaG045614
Chr8 mRNA 75067959 75074194 75067959 ID=MsaT045614.1;Parent=MsaG045614
Chr8 exon 75067959 75067992 75067959 ID=MsaT045614.1.exon6;Parent=MsaT045614.1
Chr8 CDS 75067959 75067992 75067959 ID=cds.MsaT045614.1;Parent=MsaT045614.1
Chr8 exon 75068158 75068294 75068158 ID=MsaT045614.1.exon5;Parent=MsaT045614.1
Chr8 CDS 75068158 75068294 75068158 ID=cds.MsaT045614.1;Parent=MsaT045614.1
Chr8 exon 75068400 75068500 75068400 ID=MsaT045614.1.exon4;Parent=MsaT045614.1
Chr8 CDS 75068400 75068500 75068400 ID=cds.MsaT045614.1;Parent=MsaT045614.1
Chr8 exon 75068531 75068731 75068531 ID=MsaT045614.1.exon3;Parent=MsaT045614.1
Chr8 CDS 75068531 75068731 75068531 ID=cds.MsaT045614.1;Parent=MsaT045614.1
Chr8 exon 75070462 75070582 75070462 ID=MsaT045614.1.exon2;Parent=MsaT045614.1
Chr8 CDS 75070462 75070582 75070462 ID=cds.MsaT045614.1;Parent=MsaT045614.1
Chr8 exon 75073790 75074194 75073790 ID=MsaT045614.1.exon1;Parent=MsaT045614.1
Chr8 CDS 75073790 75074194 75073790 ID=cds.MsaT045614.1;Parent=MsaT045614.1
Gene Sequence

>MsaT045614.1

ATGGCTTTTCACGAGCATCTCCAACAAGAAATAGCTTTCCAGCGTTTCACAGAAGAAGAAGATGAAGAAAGGGCATCCTGGCACAACAACAACAGCAGTGTAAACGCACGTCAACAGAATTTCATGCAAATGGAACCAGAAAGGAGGGAAAAAAGCATGGATCGGAACCACCGGAATGACAACAATTGCGAAACGGAAGAATTAAGAGAATACAAAGCAGAGATATTAGGACATCCACTCTATGATCAATTATTATCTGCTCATGTTTCGTGTCTCAGAATTGCGACACCGGTTGATCAGCTTCCAAGGATTGATGCTCAGCTTCAACAGGCACAACGTGTTCTTCAAAAATATTCTTCTGTTGGAATTGGAAATATGGATCCCAAGGAGCTTGATCACTTTATGACACATTATGTTCTTTTGCTTTGTGCCTTCAAAGAACAGTTGCAACAACATGTCCGTGTTCATGCCATGGAGGCAGTGATGGCTTGTTGGGATCTTGAGCAGTCTCTGCAAAGCTTGACAGGTGTATCTTCGGGTGAAGGAACAGGAGCAACAATGTCAGACGATGAAGATGAGCAGGCAGAGAGTAACGCTAGGTTATACAACGAAGGAAGCAGCCTTGATGGCGTCGATACTCTTGGCTTTGGTCCTCTAGTCCCCACAGAGACTGAAAGGTCTTTGATGGAACGAGTTAGGCTTGAGTTGAAGCATGAACTCAAACAAGAAGAAATTCTACGAAAGAGAAGAGCTGGGAAACTCCCTGGGGACACCACATCCCTTTTGAAAGCTTGGTGGCAATCACATTCTAAATGGCCCTATCCAACTGAGGAAGATAAGGCTAGATTGGTGCAGGAAACAGGCTTGCAATTAAAGCAAATAAATAACTGGTTTATCAATCAAAGGAAAAGGAACTGGCATGCTAACTCCCCATCCTCTAGTAGCTCCAAAACCAAGCGCAAGAGTGCAGGCGATAGCAGTAACCAGAGCTTCATGTGA
Protein sequence

>MsaT045614.1

MAFHEHLQQEIAFQRFTEEEDEERASWHNNNSSVNARQQNFMQMEPERREKSMDRNHRNDNNCETEELREYKAEILGHPLYDQLLSAHVSCLRIATPVDQLPRIDAQLQQAQRVLQKYSSVGIGNMDPKELDHFMTHYVLLLCAFKEQLQQHVRVHAMEAVMACWDLEQSLQSLTGVSSGEGTGATMSDDEDEQAESNARLYNEGSSLDGVDTLGFGPLVPTETERSLMERVRLELKHELKQEEILRKRRAGKLPGDTTSLLKAWWQSHSKWPYPTEEDKARLVQETGLQLKQINNWFINQRKRNWHANSPSSSSSKTKRKSAGDSSNQSFM*