Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045615 | PNY09981.1 | 49.072 | 377 | 116 | 11 | 2 | 369 | 14 | 323 | 7.78e-88 | 278 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045615 | sp|Q99091|CPRF3_PETCR | 42.500 | 80 | 45 | 1 | 284 | 362 | 196 | 275 | 5.77e-13 | 72.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045615 | tr|A0A2K3P3W5|A0A2K3P3W5_TRIPR | 49.072 | 377 | 116 | 11 | 2 | 369 | 14 | 323 | 3.71e-88 | 278 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG045615 | TF | bZIP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045615 | MtrunA17_Chr1g0162721 | 49.801 | 251 | 77 | 7 | 1 | 223 | 1 | 230 | 1.18e-66 | 213 |
| MsaG045615 | MtrunA17_Chr4g0065261 | 88.764 | 89 | 10 | 0 | 28 | 116 | 1 | 89 | 6.91e-57 | 183 |
| MsaG045615 | MtrunA17_Chr4g0065261 | 89.888 | 89 | 9 | 0 | 281 | 369 | 88 | 176 | 6.53e-47 | 157 |
| MsaG045615 | MtrunA17_Chr2g0302481 | 37.452 | 259 | 87 | 9 | 112 | 368 | 3 | 188 | 4.17e-33 | 122 |
| MsaG045615 | MtrunA17_Chr8g0373571 | 40.909 | 88 | 49 | 2 | 278 | 362 | 252 | 339 | 2.21e-13 | 70.5 |
| MsaG045615 | MtrunA17_Chr2g0302501 | 58.621 | 58 | 19 | 2 | 110 | 166 | 68 | 121 | 4.75e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045615 | AT4G36730.2 | 38.750 | 80 | 48 | 1 | 284 | 362 | 220 | 299 | 2.63e-11 | 64.3 |
| MsaG045615 | AT4G36730.1 | 38.750 | 80 | 48 | 1 | 284 | 362 | 222 | 301 | 2.79e-11 | 64.3 |
Find 67 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTACAGGTTGTGAGTTTC+TGG | 0.129427 | 8:+75088059 | None:intergenic |
| CTCAGCTAGTAGAGATTTAT+TGG | 0.150152 | 8:+75088413 | None:intergenic |
| TCCCCAGTTATTAATCTTAC+AGG | 0.249858 | 8:+75088045 | None:intergenic |
| GTAAATAATTGGTTGGAATC+TGG | 0.251335 | 8:+75088081 | None:intergenic |
| GAGAACAAGGATTTATCTTT+TGG | 0.256634 | 8:-75086840 | MsaT045615.1:CDS |
| GTGAGTTTCTGGTAAATAAT+TGG | 0.270018 | 8:+75088070 | None:intergenic |
| CAAAGAAATTGTTGTAATAC+TGG | 0.291279 | 8:+75087947 | None:intergenic |
| ACTGGGGAATCAAGTGAATA+TGG | 0.297191 | 8:-75088030 | MsaT045615.1:CDS |
| GAGAACAAGGTTAAGGAAAA+AGG | 0.303213 | 8:-75085165 | MsaT045615.1:intron |
| TGCAGGTGGATGATTATAAT+AGG | 0.311712 | 8:+75088693 | None:intergenic |
| CAACCTGTAAGATTAATAAC+TGG | 0.342383 | 8:-75088048 | MsaT045615.1:CDS |
| TGATGCTCAACTAAAATATA+TGG | 0.343775 | 8:-75086557 | MsaT045615.1:intron |
| TGCAATTGCTTTCTCAGAAA+TGG | 0.357172 | 8:-75088130 | MsaT045615.1:CDS |
| GGATGATTATAATAGGGATT+TGG | 0.374399 | 8:+75088700 | None:intergenic |
| CTTGGCTGATTCAGAGTTTG+AGG | 0.379924 | 8:-75086615 | MsaT045615.1:CDS |
| TGTGGCAGCATAGGATTGTT+TGG | 0.381160 | 8:+75089059 | None:intergenic |
| ATCTGTGCTGAGTCAAACTT+TGG | 0.386345 | 8:-75087914 | MsaT045615.1:CDS |
| TAAATAATTGGTTGGAATCT+GGG | 0.388774 | 8:+75088082 | None:intergenic |
| TGACGCTCGACTAAATAATA+TGG | 0.389843 | 8:-75087866 | MsaT045615.1:intron |
| AAGTTATTCTGAGGCATGTC+TGG | 0.390063 | 8:-75084179 | MsaT045615.1:CDS |
| GAAGAGCTAATTAAGGAGTA+TGG | 0.396074 | 8:-75083703 | MsaT045615.1:CDS |
| CAATCCTATGCTGCCACATT+GGG | 0.402800 | 8:-75089054 | MsaT045615.1:CDS |
| AGAGATCTAAGATGAATGTT+GGG | 0.413041 | 8:-75086869 | MsaT045615.1:CDS |
| TCAGTACATGTGGCAAAATC+AGG | 0.421341 | 8:-75088590 | MsaT045615.1:intron |
| GATTTATTGGAGAGCTTCAC+AGG | 0.424141 | 8:+75088426 | None:intergenic |
| GATCTCTGCTTCTTACATAT+AGG | 0.425971 | 8:+75086885 | None:intergenic |
| ACATTGGGCTTCATCTATGC+AGG | 0.450861 | 8:-75089039 | MsaT045615.1:intron |
| CAGAGATCTAAGATGAATGT+TGG | 0.451770 | 8:-75086870 | MsaT045615.1:CDS |
| GGAACAGGAAGAGCTAATTA+AGG | 0.452723 | 8:-75083710 | MsaT045615.1:intron |
| CAGCTAAGAGAACAAGGTTA+AGG | 0.453072 | 8:-75085172 | MsaT045615.1:CDS |
| AAAGAAGAATCCGATCCATC+TGG | 0.459016 | 8:-75089219 | MsaT045615.1:CDS |
| TCTGTGCTGAGTCAAACTTT+GGG | 0.467374 | 8:-75087913 | MsaT045615.1:CDS |
| GCAGGTGGATGATTATAATA+GGG | 0.469270 | 8:+75088694 | None:intergenic |
| AAAGTTTGACTCAGCACAGA+TGG | 0.498013 | 8:+75087916 | None:intergenic |
| GTTTCTGGTAAATAATTGGT+TGG | 0.499066 | 8:+75088074 | None:intergenic |
| GTTAAAATGATGAACCTCAA+TGG | 0.499573 | 8:+75084135 | None:intergenic |
| TTGAATCATCAGTATGCAGC+TGG | 0.509107 | 8:-75088628 | MsaT045615.1:CDS |
| CTGAGTCAAACTTTGGGGAC+AGG | 0.511343 | 8:-75087907 | MsaT045615.1:CDS |
| CTTTCTCAGAAATGGTAAGC+AGG | 0.515920 | 8:-75088122 | MsaT045615.1:CDS |
| TGAGGTACCACAAAATAGTA+AGG | 0.518570 | 8:+75089192 | None:intergenic |
| ACAATCCTATGCTGCCACAT+TGG | 0.521704 | 8:-75089055 | MsaT045615.1:CDS |
| AAACTCTGAATCAGCCAAGA+TGG | 0.521783 | 8:+75086619 | None:intergenic |
| GAAGCCCAATGTGGCAGCAT+AGG | 0.526989 | 8:+75089050 | None:intergenic |
| TATAATCATCCACCTGCAGC+TGG | 0.532701 | 8:-75088688 | MsaT045615.1:CDS |
| CAGTTACAATGTCAAGCAGC+AGG | 0.542255 | 8:+75083646 | None:intergenic |
| AACCTGTAAGATTAATAACT+GGG | 0.551341 | 8:-75088047 | MsaT045615.1:CDS |
| AAATAGTAAGGTCTTCCAGA+TGG | 0.566947 | 8:+75089204 | None:intergenic |
| AACTTTGGGGACAGGCGTCA+AGG | 0.567206 | 8:-75087899 | MsaT045615.1:CDS |
| TGATGAAAACGATTCCATTG+AGG | 0.568849 | 8:-75084149 | MsaT045615.1:intron |
| CTCATGTAAGAACCAGCTGC+AGG | 0.577655 | 8:+75088676 | None:intergenic |
| TGTTGGGGATGGTGAGAACA+AGG | 0.580631 | 8:-75086853 | MsaT045615.1:CDS |
| ATAGGGATTTGGAGTAAACT+CGG | 0.582242 | 8:+75088711 | None:intergenic |
| GTAAGGTCTTCCAGATGGAT+CGG | 0.588408 | 8:+75089209 | None:intergenic |
| CTGTGCTGAGTCAAACTTTG+GGG | 0.590960 | 8:-75087912 | MsaT045615.1:CDS |
| GAAATCTAAGATGACTGTTG+AGG | 0.593683 | 8:-75086805 | MsaT045615.1:CDS |
| TAGGGATTTGGAGTAAACTC+GGG | 0.602326 | 8:+75088712 | None:intergenic |
| CATGATTCACTGACATCTTG+AGG | 0.602539 | 8:+75089174 | None:intergenic |
| CAATAAATCTCTACTAGCTG+AGG | 0.606627 | 8:-75088412 | MsaT045615.1:intron |
| GGGAATCAGCTAAGAGAACA+AGG | 0.611347 | 8:-75085178 | MsaT045615.1:CDS |
| GAGTTTGAGGACAGTCGTCA+AGG | 0.611746 | 8:-75086602 | MsaT045615.1:CDS |
| TCTAAGATGAATGTTGGGGA+TGG | 0.626712 | 8:-75086864 | MsaT045615.1:CDS |
| ACTTGAATCATCAGTACATG+TGG | 0.630018 | 8:-75088600 | MsaT045615.1:CDS |
| ATGTAAGAACCAGCTGCAGG+TGG | 0.634873 | 8:+75088679 | None:intergenic |
| GAGATCTAAGATGAATGTTG+GGG | 0.640487 | 8:-75086868 | MsaT045615.1:CDS |
| TCTAAGATGACTGTTGAGGA+TGG | 0.650064 | 8:-75086801 | MsaT045615.1:CDS |
| ACCTGTAAGATTAATAACTG+GGG | 0.682506 | 8:-75088046 | MsaT045615.1:CDS |
| TGCATAGATGAAGCCCAATG+TGG | 0.703015 | 8:+75089041 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 75083657 | 75089247 | 75083657 | ID=MsaG045615 |
| Chr8 | mRNA | 75083657 | 75089247 | 75083657 | ID=MsaT045615.1;Parent=MsaG045615 |
| Chr8 | exon | 75083657 | 75083725 | 75083657 | ID=MsaT045615.1.exon10;Parent=MsaT045615.1 |
| Chr8 | CDS | 75083657 | 75083725 | 75083657 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75084150 | 75084272 | 75084150 | ID=MsaT045615.1.exon9;Parent=MsaT045615.1 |
| Chr8 | CDS | 75084150 | 75084272 | 75084150 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75085166 | 75085243 | 75085166 | ID=MsaT045615.1.exon8;Parent=MsaT045615.1 |
| Chr8 | CDS | 75085166 | 75085243 | 75085166 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75086558 | 75086646 | 75086558 | ID=MsaT045615.1.exon7;Parent=MsaT045615.1 |
| Chr8 | CDS | 75086558 | 75086646 | 75086558 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75086796 | 75086913 | 75086796 | ID=MsaT045615.1.exon6;Parent=MsaT045615.1 |
| Chr8 | CDS | 75086796 | 75086913 | 75086796 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75087867 | 75088157 | 75087867 | ID=MsaT045615.1.exon5;Parent=MsaT045615.1 |
| Chr8 | CDS | 75087867 | 75088157 | 75087867 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75088413 | 75088487 | 75088413 | ID=MsaT045615.1.exon4;Parent=MsaT045615.1 |
| Chr8 | CDS | 75088413 | 75088487 | 75088413 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75088591 | 75088746 | 75088591 | ID=MsaT045615.1.exon3;Parent=MsaT045615.1 |
| Chr8 | CDS | 75088591 | 75088746 | 75088591 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75089040 | 75089090 | 75089040 | ID=MsaT045615.1.exon2;Parent=MsaT045615.1 |
| Chr8 | CDS | 75089040 | 75089090 | 75089040 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Chr8 | exon | 75089188 | 75089247 | 75089188 | ID=MsaT045615.1.exon1;Parent=MsaT045615.1 |
| Chr8 | CDS | 75089188 | 75089247 | 75089188 | ID=cds.MsaT045615.1;Parent=MsaT045615.1 |
| Gene Sequence |
| Protein sequence |
