AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045687

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045687 MtrunA17_Chr4g0066271 96.765 340 9 2 1 339 74 412 0.0 677
MsaG045687 MtrunA17_Chr7g0250201 54.545 319 98 7 37 317 39 348 2.05e-84 260
MsaG045687 MtrunA17_Chr5g0402321 42.636 129 60 2 16 142 6 122 5.45e-29 111
MsaG045687 MtrunA17_Chr3g0082261 26.667 315 158 9 32 312 26 301 4.02e-19 86.7
MsaG045687 MtrunA17_Chr4g0041151 34.328 134 79 5 15 143 2 131 3.47e-14 72.4
MsaG045687 MtrunA17_Chr1g0179531 37.143 105 62 2 44 148 133 233 1.18e-13 71.6
MsaG045687 MtrunA17_Chr2g0283871 29.907 107 71 3 32 135 9 114 1.59e-11 64.3
MsaG045687 MtrunA17_Chr8g0344681 34.066 91 55 1 18 103 37 127 3.07e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045687 AT2G44730.1 47.401 327 105 8 35 317 55 358 5.80e-83 256
MsaG045687 AT3G24860.1 30.450 289 140 8 37 309 58 301 1.70e-29 115
MsaG045687 AT4G17060.1 53.425 73 34 0 253 325 235 307 9.77e-19 85.9
MsaG045687 AT3G58630.1 33.333 117 69 1 43 150 25 141 2.97e-16 79.0
MsaG045687 AT3G58630.2 33.333 117 69 1 43 150 25 141 3.02e-16 78.6
MsaG045687 AT3G14180.1 33.103 145 81 4 2 135 33 172 4.89e-15 76.3
MsaG045687 AT3G10030.1 40.217 92 51 2 44 135 161 248 1.60e-13 71.6
MsaG045687 AT3G10030.2 40.217 92 51 2 44 135 161 248 1.87e-13 71.6
MsaG045687 AT3G54390.1 32.692 104 63 1 38 141 32 128 3.49e-11 63.5
MsaG045687 AT3G54390.2 32.692 104 63 1 38 141 48 144 3.83e-11 63.5

Find 110 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATTCAGATTAGGATTA+GGG 0.281845 8:-75918223 None:intergenic
GTGGGTTCACTTTAAGCTTA+TGG 0.284555 8:+75917917 MsaT045687.1:CDS
GCGGGGAGAGCGAGAGGGAG+GGG 0.290010 8:-75917603 None:intergenic
TTGGTGGAGGATTTAGGATT+AGG 0.294445 8:+75918150 MsaT045687.1:CDS
GCAACGGCCACGAAGGGATT+GGG 0.306955 8:+75918266 MsaT045687.1:CDS
GATGGAATTCTCTTATTGAT+AGG 0.313357 8:-75918527 None:intergenic
GGAGGAGTGGTGGTTGAAGG+AGG 0.317249 8:-75917552 None:intergenic
ATGAGAGCTAGTGTTTCTTC+AGG 0.341409 8:-75917678 None:intergenic
GAGCGGGGAGAGCGAGAGGG+AGG 0.348224 8:-75917605 None:intergenic
GTGGGATAGGGAGTGGAGCG+GGG 0.351518 8:-75917620 None:intergenic
GGTGGGATAGGGAGTGGAGC+GGG 0.351713 8:-75917621 None:intergenic
AGGAGGGAGACGGCGGGTGG+AGG 0.351853 8:-75917643 None:intergenic
CGGGGAGAGCGAGAGGGAGG+GGG 0.352085 8:-75917602 None:intergenic
ATCGGAGAGAGGAGGAGGAG+TGG 0.352089 8:-75917565 None:intergenic
GGGTGATGGGTTTGTGAGAA+TGG 0.354314 8:+75918343 MsaT045687.1:CDS
TTCTGATTCAGATTAGGATT+AGG 0.360137 8:-75918224 None:intergenic
GGGAGGAGGGAGACGGCGGG+TGG 0.360567 8:-75917646 None:intergenic
GTGGTGTTGGTGGAGGATTT+AGG 0.367259 8:+75918144 MsaT045687.1:CDS
CGGCGGGTGGAGGGTGGGAT+AGG 0.369593 8:-75917633 None:intergenic
GGAGGAGGAGTGGTGGTTGA+AGG 0.374630 8:-75917555 None:intergenic
AACCCACGCCGAATTGAATC+TGG 0.390473 8:-75917901 None:intergenic
GGGAGACGGCGGGTGGAGGG+TGG 0.391865 8:-75917639 None:intergenic
GGCAACGGCCACGAAGGGAT+TGG 0.405107 8:+75918265 MsaT045687.1:CDS
TGGTAGTTCTGATTCAGATT+AGG 0.405176 8:-75918230 None:intergenic
AACGGTTTCGGTGGCGGAAT+CGG 0.412071 8:+75918095 MsaT045687.1:CDS
CCCTTCGTGGCCGTTGCCAT+TGG 0.420642 8:-75918260 None:intergenic
TTCGTGGCCGTTGCCATTGG+TGG 0.421581 8:-75918257 None:intergenic
GTGGAGGGTGGGATAGGGAG+TGG 0.424548 8:-75917627 None:intergenic
ATCTTGTGACGGCATTGAAC+AGG 0.427497 8:-75917819 None:intergenic
CTTGTTCCCCAATCCCTTCG+TGG 0.427969 8:-75918273 None:intergenic
TTCGGTGGCGGAATCGGTGG+TGG 0.431601 8:+75918101 MsaT045687.1:CDS
CTTTAAGCTTATGGATTCCA+TGG 0.436353 8:+75917926 MsaT045687.1:CDS
GGTAGTAGTGGTGTTGGTGG+AGG 0.441731 8:+75918137 MsaT045687.1:CDS
TGTATCTGCGATTAAATTGT+TGG 0.442896 8:+75918322 MsaT045687.1:CDS
TATAAGAACGGTTTCGGTGG+CGG 0.444102 8:+75918089 MsaT045687.1:CDS
GAGGAGTGGTGGTTGAAGGA+GGG 0.444252 8:-75917551 None:intergenic
GGTGGTAGTAGTGGTGTTGG+TGG 0.445358 8:+75918134 MsaT045687.1:CDS
CCGTTGCCATTGGTGGAGTA+TGG 0.446576 8:-75918250 None:intergenic
TCCTCATTCCAGATTCAATT+CGG 0.448751 8:+75917893 MsaT045687.1:CDS
TCAGGGGACCAGCAGGGAGG+AGG 0.449042 8:-75917660 None:intergenic
AAGTTGTATAAGAACGGTTT+CGG 0.449906 8:+75918083 MsaT045687.1:CDS
GGAGGGAGACGGCGGGTGGA+GGG 0.450430 8:-75917642 None:intergenic
GCCGTCTCCCTCCTCCCTGC+TGG 0.452920 8:+75917652 MsaT045687.1:CDS
GGGTGGGATAGGGAGTGGAG+CGG 0.454564 8:-75917622 None:intergenic
AGGAGTGGTGGTTGAAGGAG+GGG 0.455601 8:-75917550 None:intergenic
CGATTAAATTGTTGGGTGAT+GGG 0.458197 8:+75918330 MsaT045687.1:CDS
TGTTTCTTCAGGGGACCAGC+AGG 0.459413 8:-75917667 None:intergenic
AGTGGAGCGGGGAGAGCGAG+AGG 0.473996 8:-75917609 None:intergenic
GTCACAAGATGGAGAAGCTT+CGG 0.475663 8:+75917832 MsaT045687.1:CDS
GGAGACGGCGGGTGGAGGGT+GGG 0.478675 8:-75917638 None:intergenic
TGAGAGCTAGTGTTTCTTCA+GGG 0.479128 8:-75917677 None:intergenic
GTATCTGCGATTAAATTGTT+GGG 0.482195 8:+75918323 MsaT045687.1:CDS
GGTGGTGGTAGTAGTGGTGT+TGG 0.485391 8:+75918131 MsaT045687.1:CDS
CATTATGATTGTTGTGGTGA+TGG 0.489229 8:-75918545 None:intergenic
ATCAATAAGTTGTATAAGAA+CGG 0.492654 8:+75918077 MsaT045687.1:CDS
CAGGGGACCAGCAGGGAGGA+GGG 0.493621 8:-75917659 None:intergenic
GTTGAAGGAGGGGAAGACAT+TGG 0.494183 8:-75917540 None:intergenic
CGTGACAAATGGTACTCTCT+TGG 0.494378 8:+75917711 MsaT045687.1:CDS
GAGAATGGAGCAAAAGAAGA+TGG 0.500362 8:+75918358 MsaT045687.1:CDS
GGAGAGAGGAGGAGGAGTGG+TGG 0.506258 8:-75917562 None:intergenic
ACCAGCAGGGAGGAGGGAGA+CGG 0.506473 8:-75917653 None:intergenic
GCGATTAAATTGTTGGGTGA+TGG 0.506498 8:+75918329 MsaT045687.1:CDS
GCAGATACAACCTCAGTCAA+TGG 0.509994 8:-75918308 None:intergenic
GCAGGGAGGAGGGAGACGGC+GGG 0.511588 8:-75917649 None:intergenic
GGCGGGTGGAGGGTGGGATA+GGG 0.512182 8:-75917632 None:intergenic
GTAATCTCAAAGCCACTCAC+TGG 0.525271 8:+75917739 MsaT045687.1:CDS
AGCGGGGAGAGCGAGAGGGA+GGG 0.526052 8:-75917604 None:intergenic
GCTGTCATCGGAAACTGCCT+TGG 0.529999 8:-75918477 None:intergenic
GGTTTCGGTGGCGGAATCGG+TGG 0.531244 8:+75918098 MsaT045687.1:CDS
AGCGACTTCTTGCCAGTGAG+TGG 0.532762 8:-75917751 None:intergenic
TCGACTCCTACCGTGACAAA+TGG 0.536305 8:+75917700 MsaT045687.1:CDS
ACTGTTGTTATTGCTGTCAT+CGG 0.538503 8:-75918489 None:intergenic
TGAAACTATGAGGATGGAGA+TGG 0.538920 8:+75918397 MsaT045687.1:CDS
CGAAGGGATTGGGGAACAAG+AGG 0.540694 8:+75918276 MsaT045687.1:CDS
GTGGAGCGGGGAGAGCGAGA+GGG 0.543189 8:-75917608 None:intergenic
TTTGATTCAGGTTGTGCCGG+TGG 0.545116 8:-75918176 None:intergenic
AGCAGGGAGGAGGGAGACGG+CGG 0.547257 8:-75917650 None:intergenic
TGTTCAATGCCGTCACAAGA+TGG 0.547819 8:+75917821 MsaT045687.1:CDS
CGAGGGAGATTGAAACTATG+AGG 0.548584 8:+75918387 MsaT045687.1:CDS
TTGTATAAGAACGGTTTCGG+TGG 0.552376 8:+75918086 MsaT045687.1:CDS
CAACGGCCACGAAGGGATTG+GGG 0.553536 8:+75918267 MsaT045687.1:CDS
CTTATGGATTCCATGGAGAA+AGG 0.553692 8:+75917933 MsaT045687.1:CDS
TGAATCTCAACAGCGAATTG+TGG 0.554265 8:+75918445 MsaT045687.1:CDS
TTTAGTGGTGGTGGTAGTAG+TGG 0.554628 8:+75918125 MsaT045687.1:CDS
TTCCAGATTCAATTCGGCGT+GGG 0.557065 8:+75917899 MsaT045687.1:CDS
ACCAATGGCAACGGCCACGA+AGG 0.566202 8:+75918259 MsaT045687.1:CDS
AAAAGAAGATGGAGATGGCG+AGG 0.571525 8:+75918369 MsaT045687.1:CDS
CCAATGGCAACGGCCACGAA+GGG 0.574376 8:+75918260 MsaT045687.1:CDS
GAAGCTTCTCCATCTTGTGA+CGG 0.577882 8:-75917830 None:intergenic
ATTTAGGATTAGGTATCCAC+CGG 0.578430 8:+75918160 MsaT045687.1:CDS
GGAGATTGAAACTATGAGGA+TGG 0.585982 8:+75918391 MsaT045687.1:CDS
GTTTCTTCAGGGGACCAGCA+GGG 0.594344 8:-75917666 None:intergenic
AAATGGTACTCTCTTGGCCG+CGG 0.594791 8:+75917717 MsaT045687.1:CDS
GAAGGGATTGGGGAACAAGA+GGG 0.597432 8:+75918277 MsaT045687.1:CDS
CCATACTCCACCAATGGCAA+CGG 0.603853 8:+75918250 MsaT045687.1:CDS
GCCGAATTGAATCTGGAATG+AGG 0.606212 8:-75917894 None:intergenic
AGGAGAAGGACCTTTCTCCA+TGG 0.610673 8:-75917943 None:intergenic
AAAGAAGATGGAGATGGCGA+GGG 0.614159 8:+75918370 MsaT045687.1:CDS
GTCGAACATTATGATTGTTG+TGG 0.614285 8:-75918551 None:intergenic
ATCTCAACAGCGAATTGTGG+AGG 0.620844 8:+75918448 MsaT045687.1:CDS
GAACTACCATACTCCACCAA+TGG 0.622768 8:+75918244 MsaT045687.1:CDS
AGAGTACCATTTGTCACGGT+AGG 0.626402 8:-75917706 None:intergenic
ATTCCAGATTCAATTCGGCG+TGG 0.627728 8:+75917898 MsaT045687.1:CDS
GAGAGCTAGTGTTTCTTCAG+GGG 0.629054 8:-75917676 None:intergenic
AATTGTGGAGGCATTCGCCA+AGG 0.638281 8:+75918460 MsaT045687.1:CDS
GGAGCAAAAGAAGATGGAGA+TGG 0.642155 8:+75918364 MsaT045687.1:CDS
CAAGAGAGTACCATTTGTCA+CGG 0.645825 8:-75917710 None:intergenic
GGAGAGAGATCCATTGACTG+AGG 0.657971 8:+75918298 MsaT045687.1:CDS
TCTTCAGGGGACCAGCAGGG+AGG 0.693934 8:-75917663 None:intergenic
GAGTGGCTTTGAGATTACCG+CGG 0.712719 8:-75917734 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 75917543 75918565 75917543 ID=MsaG045687
Chr8 mRNA 75917543 75918565 75917543 ID=MsaT045687.1;Parent=MsaG045687
Chr8 exon 75917543 75918565 75917543 ID=MsaT045687.1.exon1;Parent=MsaT045687.1
Chr8 CDS 75917543 75918565 75917543 ID=cds.MsaT045687.1;Parent=MsaT045687.1
Gene Sequence

>MsaT045687.1

ATGTCTTCCCCTCCTTCAACCACCACTCCTCCTCCTCTCTCCGATCCCCCCACCGCCGTCCCCCTCCCTCTCGCTCTCCCCGCTCCACTCCCTATCCCACCCTCCACCCGCCGTCTCCCTCCTCCCTGCTGGTCCCCTGAAGAAACACTAGCTCTCATCGACTCCTACCGTGACAAATGGTACTCTCTTGGCCGCGGTAATCTCAAAGCCACTCACTGGCAAGAAGTCGCTGACGCTGTTTCAAATCGTTGCCCTAACGTTACTCCGTCGAAAACTCCTGTTCAATGCCGTCACAAGATGGAGAAGCTTCGGAAACGTTATCGTACTGAGATTCAACGTGCTCGCTCGCTTCCTCATTCCAGATTCAATTCGGCGTGGGTTCACTTTAAGCTTATGGATTCCATGGAGAAAGGTCCTTCTCCTGTTAAATCTGAAAACAATGATTCTGATAGTGCTGATGATGATGAAGATGAAGATCATGATCAAAATCTGTATTTAGATATCAAAAACGGTCATGGATCTAATACTAGAAGTATCAATAAGTTGTATAAGAACGGTTTCGGTGGCGGAATCGGTGGTGGTTTTAGTGGTGGTGGTAGTAGTGGTGTTGGTGGAGGATTTAGGATTAGGTATCCACCGGCACAACCTGAATCAAAATTTTCTTCTGATCAGAAATATAACCCTAATCCTAATCTGAATCAGAACTACCATACTCCACCAATGGCAACGGCCACGAAGGGATTGGGGAACAAGAGGGAGAGAGATCCATTGACTGAGGTTGTATCTGCGATTAAATTGTTGGGTGATGGGTTTGTGAGAATGGAGCAAAAGAAGATGGAGATGGCGAGGGAGATTGAAACTATGAGGATGGAGATGGAAATGAAACGTACTGAGATGATTCTTGAATCTCAACAGCGAATTGTGGAGGCATTCGCCAAGGCAGTTTCCGATGACAGCAATAACAACAGTAACAAGATGATGAAGCCTATCAATAAGAGAATTCCATCACCACAACAATCATAA
Protein sequence

>MsaT045687.1

MSSPPSTTTPPPLSDPPTAVPLPLALPAPLPIPPSTRRLPPPCWSPEETLALIDSYRDKWYSLGRGNLKATHWQEVADAVSNRCPNVTPSKTPVQCRHKMEKLRKRYRTEIQRARSLPHSRFNSAWVHFKLMDSMEKGPSPVKSENNDSDSADDDEDEDHDQNLYLDIKNGHGSNTRSINKLYKNGFGGGIGGGFSGGGSSGVGGGFRIRYPPAQPESKFSSDQKYNPNPNLNQNYHTPPMATATKGLGNKRERDPLTEVVSAIKLLGDGFVRMEQKKMEMAREIETMRMEMEMKRTEMILESQQRIVEAFAKAVSDDSNNNSNKMMKPINKRIPSPQQS*