Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045723 | AES91783.1 | 91.713 | 181 | 15 | 0 | 1 | 181 | 1 | 181 | 3.22e-113 | 329 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045723 | tr|G7JJ33|G7JJ33_MEDTR | 91.713 | 181 | 15 | 0 | 1 | 181 | 1 | 181 | 1.54e-113 | 329 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG045723 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG042721 | MsaG045723 | 0.808541 | 5.014948e-50 | 8.268084e-48 |
| MsaG019435 | MsaG045723 | 0.809534 | 3.081378e-50 | 5.203337e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045723 | MtrunA17_Chr4g0066461 | 45.946 | 111 | 41 | 3 | 77 | 181 | 1 | 98 | 3.68e-21 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGCTCTGTTACGGTTAC+TGG | 0.180827 | 8:+76298103 | MsaT045723.1:CDS |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8:+76298127 | MsaT045723.1:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8:+76298074 | MsaT045723.1:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8:+76298312 | MsaT045723.1:CDS |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8:+76298128 | MsaT045723.1:CDS |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8:+76298268 | MsaT045723.1:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8:+76297664 | MsaT045723.1:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8:+76298258 | MsaT045723.1:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8:+76297665 | MsaT045723.1:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8:-76297793 | None:intergenic |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8:+76298243 | MsaT045723.1:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8:+76298012 | MsaT045723.1:CDS |
| GAATCTCAATTGCTCTGTTA+CGG | 0.448731 | 8:+76298096 | MsaT045723.1:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8:-76297794 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8:+76298042 | MsaT045723.1:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8:+76298000 | MsaT045723.1:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8:+76298229 | MsaT045723.1:CDS |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8:+76298141 | MsaT045723.1:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8:-76297778 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8:+76298151 | MsaT045723.1:CDS |
| TTCAACGTTTAGAACCTCTT+CGG | 0.524218 | 8:-76297717 | None:intergenic |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8:+76298040 | MsaT045723.1:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8:+76298003 | MsaT045723.1:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8:+76298041 | MsaT045723.1:CDS |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8:+76297729 | MsaT045723.1:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8:+76298043 | MsaT045723.1:CDS |
| CACTGCTACTAAAACCGAAG+AGG | 0.637311 | 8:+76297703 | MsaT045723.1:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8:-76298169 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8:+76298117 | MsaT045723.1:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8:-76297786 | None:intergenic |
| TTGAGATTCCTATTACGACA+CGG | 0.693765 | 8:-76298082 | None:intergenic |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8:+76297680 | MsaT045723.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 76297650 | 76298333 | 76297650 | ID=MsaG045723 |
| Chr8 | mRNA | 76297650 | 76298333 | 76297650 | ID=MsaT045723.1;Parent=MsaG045723 |
| Chr8 | exon | 76297650 | 76297811 | 76297650 | ID=MsaT045723.1.exon1;Parent=MsaT045723.1 |
| Chr8 | CDS | 76297650 | 76297811 | 76297650 | ID=cds.MsaT045723.1;Parent=MsaT045723.1 |
| Chr8 | exon | 76297950 | 76298333 | 76297950 | ID=MsaT045723.1.exon2;Parent=MsaT045723.1 |
| Chr8 | CDS | 76297950 | 76298333 | 76297950 | ID=cds.MsaT045723.1;Parent=MsaT045723.1 |
| Gene Sequence |
| Protein sequence |
