AlfalfaGEDB | MsaG045739

M. sativa ssp. caerulea / MsaG045739

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00642.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045739	XP_003609623.2	96.673	511	6	2	1	500	1	511	0.0	967

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045739	sp\|Q6NPN3\|C3H58_ARATH	51.772	508	184	15	3	493	2	465	1.27e-144	426

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045739	tr\|G7JKA0\|G7JKA0_MEDTR	96.673	511	6	2	1	500	1	511	0.0	967

TFs/TRs:

Gene ID	Type	Classification
MsaG045739	TF	C3H

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000385	MsaG045739	0.803579	5.488726e-49	8.051497e-47
MsaG000408	MsaG045739	0.803847	4.831821e-49	7.131988e-47
MsaG000613	MsaG045739	0.810748	1.691879e-50	2.942355e-48
MsaG001133	MsaG045739	0.801840	1.249029e-48	1.760546e-46
MsaG002481	MsaG045739	0.825642	7.451265e-54	1.907779e-51
MsaG002885	MsaG045739	0.839057	3.664799e-57	1.389164e-54
MsaG004280	MsaG045739	0.801342	1.578872e-48	2.200422e-46
MsaG004654	MsaG045739	0.813799	3.676909e-51	6.896648e-49
MsaG004794	MsaG045739	0.827358	2.918185e-54	7.837615e-52
MsaG005728	MsaG045739	0.822026	5.197185e-53	1.206087e-50
MsaG005843	MsaG045739	0.815067	1.933667e-51	3.744266e-49
MsaG006240	MsaG045739	0.800741	2.091941e-48	2.876222e-46
MsaG007584	MsaG045739	0.801850	1.243572e-48	1.753230e-46
MsaG007734	MsaG045739	0.803161	6.693768e-49	9.724904e-47
MsaG009117	MsaG045739	0.804336	3.826716e-49	5.713045e-47
MsaG009531	MsaG045739	0.813716	3.833845e-51	7.175757e-49
MsaG010644	MsaG045739	0.814198	3.004985e-51	5.693002e-49
MsaG010890	MsaG045739	0.807020	1.052196e-49	1.672675e-47
MsaG011481	MsaG045739	0.811324	1.270610e-50	2.241206e-48
MsaG012046	MsaG045739	0.831770	2.496464e-55	7.604218e-53
MsaG041985	MsaG045739	0.833562	9.009793e-56	2.891569e-53
MsaG043721	MsaG045739	0.810067	2.368996e-50	4.052179e-48
MsaG044546	MsaG045739	0.824078	1.735876e-53	4.258068e-51
MsaG045510	MsaG045739	0.810990	1.500287e-50	2.624657e-48
MsaG045518	MsaG045739	0.807251	9.404091e-50	1.503133e-47
MsaG045739	MsaG045717	0.818277	3.717696e-52	7.815242e-50
MsaG045739	MsaG045822	0.858804	1.252962e-62	9.310217e-60
MsaG045739	MsaG045986	0.825608	7.589858e-54	1.941421e-51
MsaG045739	MsaG046054	0.807333	9.037736e-50	1.447425e-47
MsaG045739	MsaG046643	0.809791	2.714736e-50	4.612741e-48
MsaG045739	MsaG046660	0.835874	2.376296e-56	8.170832e-54
MsaG045739	MsaG009124	0.821465	6.995964e-53	1.599444e-50
MsaG045739	MsaG014278	0.803472	5.775734e-49	8.451533e-47
MsaG045739	MsaG012667	0.804597	3.379084e-49	5.075051e-47
MsaG045739	MsaG013546	0.802229	1.040219e-48	1.479245e-46
MsaG045739	MsaG014406	0.803654	5.295415e-49	7.781538e-47
MsaG045739	MsaG013859	0.818097	4.081545e-52	8.540432e-50
MsaG045739	MsaG011821	0.821885	5.599272e-53	1.294543e-50
MsaG045739	MsaG018216	0.814033	3.266206e-51	6.162103e-49
MsaG045739	MsaG019915	0.852719	7.367854e-61	4.389320e-58
MsaG045739	MsaG028782	0.808876	4.256256e-50	7.074269e-48
MsaG045739	MsaG027201	0.811900	9.539465e-51	1.706568e-48
MsaG045739	MsaG027840	0.827259	3.081797e-54	8.254042e-52
MsaG045739	MsaG032880	0.804762	3.122235e-49	4.707412e-47
MsaG045739	MsaG039788	0.812348	7.624737e-51	1.379196e-48
MsaG045739	MsaG043116	0.802762	8.083939e-49	1.163703e-46
MsaG045739	MsaG042683	0.803212	6.533393e-49	9.503123e-47
MsaG045739	MsaG043209	0.810137	2.288220e-50	3.920676e-48
MsaG012814	MsaG045739	0.810219	2.197878e-50	3.773373e-48
MsaG014290	MsaG045739	0.801254	1.645260e-48	2.288324e-46
MsaG015479	MsaG045739	0.815607	1.468425e-51	2.882641e-49
MsaG015829	MsaG045739	0.805800	1.896611e-49	2.929459e-47
MsaG016329	MsaG045739	0.846817	3.225715e-59	1.569932e-56
MsaG016974	MsaG045739	0.852769	7.129572e-61	4.254780e-58
MsaG017183	MsaG045739	0.814623	2.422861e-51	4.639220e-49
MsaG017290	MsaG045739	0.817161	6.619550e-52	1.352159e-49
MsaG017483	MsaG045739	0.813391	4.517094e-51	8.386116e-49
MsaG017529	MsaG045739	0.812408	7.398997e-51	1.340374e-48
MsaG018082	MsaG045739	0.803738	5.088799e-49	7.492667e-47
MsaG018149	MsaG045739	0.812554	6.876526e-51	1.250279e-48
MsaG018598	MsaG045739	0.818131	4.009270e-52	8.396705e-50
MsaG018735	MsaG045739	0.818561	3.207288e-52	6.792000e-50
MsaG018960	MsaG045739	0.812088	8.681739e-51	1.560254e-48
MsaG019183	MsaG045739	0.804677	3.251826e-49	4.893071e-47
MsaG019188	MsaG045739	0.820095	1.440205e-52	3.175589e-50
MsaG020245	MsaG045739	0.800137	2.773718e-48	3.761748e-46
MsaG020418	MsaG045739	0.810879	1.584882e-50	2.765166e-48
MsaG020732	MsaG045739	0.829141	1.089423e-54	3.076164e-52
MsaG020716	MsaG045739	0.822463	4.119470e-53	9.672638e-51
MsaG020834	MsaG045739	0.805670	2.019752e-49	3.110140e-47
MsaG022627	MsaG045739	0.811271	1.304929e-50	2.298699e-48
MsaG022868	MsaG045739	0.806445	1.389614e-49	2.179460e-47
MsaG023268	MsaG045739	0.830143	6.234379e-55	1.811665e-52
MsaG023416	MsaG045739	0.820677	1.060223e-52	2.373857e-50
MsaG023641	MsaG045739	0.845491	7.375043e-59	3.434375e-56
MsaG027248	MsaG045739	0.803089	6.925526e-49	1.004501e-46
MsaG027557	MsaG045739	0.842017	6.210316e-58	2.584108e-55
MsaG027590	MsaG045739	0.815422	1.613741e-51	3.153043e-49
MsaG027621	MsaG045739	0.812793	6.100055e-51	1.115679e-48
MsaG027950	MsaG045739	0.818482	3.342264e-52	7.063444e-50
MsaG028192	MsaG045739	0.802228	1.040532e-48	1.479676e-46
MsaG028343	MsaG045739	0.807209	9.596648e-50	1.532397e-47
MsaG028844	MsaG045739	0.808806	4.404699e-50	7.308583e-48
MsaG029063	MsaG045739	0.813496	4.284429e-51	7.974854e-49
MsaG029147	MsaG045739	0.814406	2.705273e-51	5.151933e-49
MsaG029464	MsaG045739	0.800537	2.301280e-48	3.149464e-46
MsaG029580	MsaG045739	0.816572	8.961398e-52	1.802903e-49
MsaG030196	MsaG045739	0.824584	1.321067e-53	3.285560e-51
MsaG030841	MsaG045739	0.808695	4.650984e-50	7.696834e-48
MsaG030899	MsaG045739	0.803762	5.031184e-49	7.411736e-47
MsaG031007	MsaG045739	0.810859	1.600711e-50	2.791431e-48
MsaG031386	MsaG045739	0.801408	1.530585e-48	2.136322e-46
MsaG031781	MsaG045739	0.842459	4.748892e-58	2.004207e-55
MsaG032491	MsaG045739	0.818712	2.965709e-52	6.305249e-50
MsaG033231	MsaG045739	0.808957	4.091648e-50	6.813792e-48
MsaG033640	MsaG045739	0.815378	1.650960e-51	3.222151e-49
MsaG034853	MsaG045739	0.822476	4.091011e-53	9.609298e-51
MsaG039573	MsaG045739	0.811857	9.744139e-51	1.741359e-48
MsaG039781	MsaG045739	0.816280	1.040887e-51	2.078587e-49
MsaG041072	MsaG045739	0.806055	1.677702e-49	2.606973e-47
MsaG041299	MsaG045739	0.810573	1.844948e-50	3.194968e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045739	MtrunA17_Chr4g0066941	96.296	513	6	3	1	500	1	513	0.0	962
MsaG045739	MtrunA17_Chr2g0286151	66.935	496	127	6	18	500	11	482	0.0	557
MsaG045739	MtrunA17_Chr2g0286151	34.810	158	69	3	270	393	10	167	8.97e-17	82.8
MsaG045739	MtrunA17_Chr1g0195891	52.785	377	127	10	5	367	4	343	3.53e-112	335
MsaG045739	MtrunA17_Chr1g0195891	49.333	75	36	1	320	392	92	166	5.49e-17	82.4
MsaG045739	MtrunA17_Chr7g0249221	44.634	410	176	13	42	441	27	395	1.75e-102	313
MsaG045739	MtrunA17_Chr5g0438891	38.950	362	158	12	42	397	36	340	1.25e-71	233
MsaG045739	MtrunA17_Chr5g0438891	48.780	82	42	0	39	120	75	156	1.19e-19	91.3
MsaG045739	MtrunA17_Chr5g0438891	42.391	92	52	1	28	119	248	338	2.22e-15	78.2
MsaG045739	MtrunA17_Chr3g0116431	35.620	379	156	9	37	394	166	477	8.18e-65	218
MsaG045739	MtrunA17_Chr3g0116431	40.964	83	42	3	320	397	172	252	2.25e-11	66.2
MsaG045739	MtrunA17_Chr4g0058011	37.500	376	153	14	38	396	227	537	7.63e-63	214
MsaG045739	MtrunA17_Chr8g0356241	32.609	368	200	10	42	392	138	474	4.75e-46	168
MsaG045739	MtrunA17_Chr8g0356241	34.463	177	78	4	21	163	167	339	5.55e-27	114
MsaG045739	MtrunA17_Chr8g0356241	35.135	185	73	4	35	173	302	485	1.26e-25	110
MsaG045739	MtrunA17_Chr8g0356241	42.683	82	39	3	320	393	139	220	1.70e-12	69.7
MsaG045739	MtrunA17_Chr1g0195901	57.303	89	35	2	396	484	1	86	2.54e-15	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045739	AT5G18550.1	51.772	508	184	15	3	493	2	465	1.29e-145	426
MsaG045739	AT5G18550.4	53.459	477	167	14	3	462	2	440	5.29e-144	421
MsaG045739	AT2G47850.1	51.316	456	171	9	20	464	16	431	1.60e-143	421
MsaG045739	AT2G47850.3	51.316	456	171	9	20	464	16	431	1.60e-143	421
MsaG045739	AT5G18550.3	53.463	462	165	13	40	493	14	433	5.22e-141	413
MsaG045739	AT5G18550.2	50.099	507	181	15	3	493	2	452	2.29e-137	404
MsaG045739	AT3G06410.1	56.481	432	139	13	3	415	2	403	3.46e-131	389
MsaG045739	AT2G47850.2	46.491	456	167	10	20	464	16	405	8.34e-119	356
MsaG045739	AT3G02830.1	44.562	377	150	8	41	408	36	362	5.38e-97	299
MsaG045739	AT3G02830.1	42.857	84	47	1	310	392	28	111	7.68e-14	73.6
MsaG045739	AT3G02830.2	44.562	377	150	8	41	408	16	342	7.69e-97	298
MsaG045739	AT3G02830.2	42.857	84	47	1	310	392	8	91	8.64e-14	73.6
MsaG045739	AT2G47850.4	50.581	344	123	8	128	464	6	309	1.02e-93	288
MsaG045739	AT2G47850.4	51.765	85	41	0	79	163	156	240	5.58e-23	100
MsaG045739	AT2G47850.4	39.773	88	44	2	34	116	156	239	8.29e-16	79.3
MsaG045739	AT1G04990.1	37.079	356	153	11	42	392	44	333	8.39e-63	210
MsaG045739	AT1G04990.1	40.833	120	69	2	305	423	73	191	4.88e-21	95.5
MsaG045739	AT1G04990.1	42.683	82	47	0	40	121	85	166	3.04e-17	84.3
MsaG045739	AT1G04990.1	37.273	110	59	3	89	195	260	362	3.98e-12	68.6
MsaG045739	AT1G04990.6	37.079	356	153	11	42	392	44	333	8.39e-63	210
MsaG045739	AT1G04990.6	40.833	120	69	2	305	423	73	191	4.88e-21	95.5
MsaG045739	AT1G04990.6	42.683	82	47	0	40	121	85	166	3.04e-17	84.3
MsaG045739	AT1G04990.6	37.273	110	59	3	89	195	260	362	3.98e-12	68.6
MsaG045739	AT1G04990.2	37.079	356	153	11	42	392	44	333	8.39e-63	210
MsaG045739	AT1G04990.2	40.833	120	69	2	305	423	73	191	4.88e-21	95.5
MsaG045739	AT1G04990.2	42.683	82	47	0	40	121	85	166	3.04e-17	84.3
MsaG045739	AT1G04990.2	37.273	110	59	3	89	195	260	362	3.98e-12	68.6
MsaG045739	AT2G32930.3	31.809	481	231	15	42	494	14	425	9.20e-62	208
MsaG045739	AT2G32930.3	39.286	84	51	0	34	117	50	133	1.01e-15	79.7
MsaG045739	AT2G32930.2	32.017	481	230	17	42	494	41	452	1.32e-61	209
MsaG045739	AT2G32930.2	39.759	83	50	0	34	116	77	159	1.14e-15	79.7
MsaG045739	AT5G63260.1	38.360	378	158	14	42	403	98	416	1.46e-60	205
MsaG045739	AT5G63260.1	38.961	77	46	1	40	115	329	405	1.10e-13	73.6
MsaG045739	AT1G04990.5	36.056	355	138	12	42	392	44	313	1.31e-58	199
MsaG045739	AT1G04990.5	40.833	120	69	2	305	423	73	191	3.97e-21	95.5
MsaG045739	AT1G04990.5	42.683	82	47	0	40	121	85	166	3.60e-17	84.0
MsaG045739	AT5G16540.1	36.012	336	162	9	23	348	23	315	1.01e-57	196
MsaG045739	AT5G16540.1	53.261	92	41	1	315	404	236	327	4.21e-26	110
MsaG045739	AT5G16540.1	51.948	77	37	0	87	163	240	316	4.45e-19	89.4
MsaG045739	AT5G16540.1	46.667	75	38	1	320	392	85	159	8.35e-17	82.4
MsaG045739	AT5G16540.1	39.394	99	55	2	318	415	38	132	1.08e-12	70.1
MsaG045739	AT2G32930.1	31.601	481	228	16	42	494	41	448	1.23e-57	198
MsaG045739	AT5G16540.3	36.012	336	162	9	23	348	2	294	1.86e-57	195
MsaG045739	AT5G16540.3	53.261	92	41	1	315	404	215	306	4.24e-26	109
MsaG045739	AT5G16540.3	51.948	77	37	0	87	163	219	295	5.49e-19	89.0
MsaG045739	AT5G16540.3	46.667	75	38	1	320	392	64	138	9.93e-17	82.0
MsaG045739	AT5G16540.3	39.394	99	55	2	318	415	17	111	1.13e-12	69.7
MsaG045739	AT5G63260.2	36.802	394	158	15	42	403	98	432	3.77e-57	197
MsaG045739	AT5G63260.2	38.961	77	46	1	40	115	345	421	1.24e-13	73.6
MsaG045739	AT3G48440.1	35.156	384	167	13	31	391	93	417	3.85e-55	191
MsaG045739	AT3G48440.1	37.500	80	49	1	40	118	341	420	3.26e-13	72.0
MsaG045739	AT3G48440.1	29.508	183	68	5	313	440	101	277	5.17e-13	71.2
MsaG045739	AT5G16540.2	35.484	341	150	11	23	348	23	308	4.31e-55	189
MsaG045739	AT5G16540.2	47.778	90	42	1	315	404	236	320	2.10e-20	93.2
MsaG045739	AT5G16540.2	46.667	75	38	1	320	392	85	159	8.17e-17	82.4
MsaG045739	AT5G16540.2	39.394	99	55	2	318	415	38	132	1.06e-12	70.1
MsaG045739	AT5G16540.4	47.179	195	85	5	23	214	2	181	8.51e-55	185
MsaG045739	AT5G16540.4	33.553	152	92	2	320	469	64	208	3.63e-17	82.0
MsaG045739	AT5G16540.4	60.000	55	20	1	315	367	215	269	2.64e-14	73.6
MsaG045739	AT5G16540.4	39.394	99	55	2	318	415	17	111	6.65e-13	69.3
MsaG045739	AT1G04990.3	33.887	301	130	10	97	392	16	252	1.01e-40	149
MsaG045739	AT1G04990.3	43.000	100	55	2	325	423	12	110	1.62e-17	84.0
MsaG045739	AT1G04990.3	43.478	69	39	0	53	121	17	85	2.11e-14	74.7
MsaG045739	AT1G04990.3	37.273	110	59	3	89	195	179	281	2.17e-12	68.6
MsaG045739	AT1G04990.4	32.667	300	115	11	97	392	16	232	1.28e-36	137
MsaG045739	AT1G04990.4	43.000	100	55	2	325	423	12	110	1.19e-17	84.0
MsaG045739	AT1G04990.4	43.478	69	39	0	53	121	17	85	2.50e-14	74.3
MsaG045739	AT3G12680.1	41.722	151	60	3	39	163	219	367	5.87e-33	132
MsaG045739	AT3G12680.1	39.779	181	63	6	34	171	329	506	7.54e-28	117
MsaG045739	AT3G12680.1	42.969	128	64	3	42	163	171	295	4.76e-27	114
MsaG045739	AT3G12680.1	48.810	84	34	1	318	392	416	499	3.75e-19	90.9
MsaG045739	AT3G12680.1	31.250	144	70	3	312	427	258	400	8.08e-16	80.5
MsaG045739	AT3G12680.1	38.614	101	54	3	320	412	172	272	1.18e-13	73.6
MsaG045739	AT3G12680.1	46.575	73	38	1	320	392	223	294	2.35e-13	72.8
MsaG045739	AT1G48195.1	46.250	80	41	1	316	393	3	82	4.97e-19	82.0
MsaG045739	AT1G48195.1	46.053	76	41	0	86	161	5	80	1.36e-16	75.1
MsaG045739	AT1G48195.1	37.662	77	47	1	40	115	4	80	1.91e-11	60.5

Find 195 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTGCTGATCAGGCCTTTC+AGG	0.127139	8:+76484816	None:intergenic
TCATTTGTCCAGGGCCCTTA+TGG	0.161593	8:-76485203	MsaT045739.1:CDS
AAAACAGGGGACTGTAAATT+TGG	0.217295	8:-76484777	MsaT045739.1:CDS
GTTGGTTCGACGCAGCTTTA+TGG	0.252629	8:-76485002	MsaT045739.1:CDS
TTGTGATTGGTTTAGGTTAT+TGG	0.258647	8:-76486079	MsaT045739.1:intron
TAAGGGCTCCAGCCTGAAAA+AGG	0.262944	8:+76485152	None:intergenic
AGGACGGGATCGTGCAAATT+TGG	0.273979	8:-76485672	MsaT045739.1:CDS
TTCAGGCTGGAGCCCTTATC+AGG	0.307461	8:-76485147	MsaT045739.1:intron
GGATGAAGCAGGCTCCTTGC+TGG	0.312902	8:+76483385	None:intergenic
CCCTTGTCGCTAAATTGTTA+TGG	0.320416	8:-76485600	MsaT045739.1:CDS
CATTTGTCCAGGGCCCTTAT+GGG	0.325235	8:-76485202	MsaT045739.1:CDS
GGCTGTACAGTTTGATAAAT+AGG	0.338710	8:+76485290	None:intergenic
ACGGGCTGTCCAACTCGTTC+TGG	0.343305	8:+76486031	None:intergenic
GCTGGTCCTTATCAATCTTC+TGG	0.343679	8:-76484885	MsaT045739.1:CDS
GCTGGTCCTTACCAATCTTC+TGG	0.357551	8:-76484915	MsaT045739.1:CDS
TCTAGGATGGTGATACTTGC+AGG	0.363592	8:+76485644	None:intergenic
GCAGGGTATGGTGGATTGGT+TGG	0.368471	8:-76486453	MsaT045739.1:intron
TTGGTTCGACGCAGCTTTAT+GGG	0.368819	8:-76485001	MsaT045739.1:CDS
TGCTGACATTCCTGCTGATC+AGG	0.370843	8:+76484807	None:intergenic
TCACCAACAACCGTCTATAC+TGG	0.371079	8:-76484963	MsaT045739.1:CDS
GACATTACCATTGGCCCATA+AGG	0.373452	8:+76485188	None:intergenic
CTGATTGTATCTATTATTTG+AGG	0.384105	8:-76486395	MsaT045739.1:CDS
CTGGATCCTGATGCAAGTTC+AGG	0.386934	8:+76483404	None:intergenic
ATTGGAGCTGCGAGGATTGC+CGG	0.388091	8:-76486061	MsaT045739.1:CDS
GCTCATTGATGATGACATTC+TGG	0.391929	8:+76483364	None:intergenic
CAACCATCTTCTCAGAGTTC+CGG	0.392756	8:-76483278	MsaT045739.1:CDS
TGTCAGCATTACATGAAAAC+AGG	0.398214	8:-76484792	MsaT045739.1:CDS
AAAGTGAACCTTAGTCCTAT+TGG	0.411454	8:-76484711	MsaT045739.1:CDS
TGAATTAACCACCTGGCAAA+CGG	0.411779	8:+76486012	None:intergenic
GGTGCTACTTGTGGTGGCAC+TGG	0.412279	8:+76483241	None:intergenic
GGTCCCTCAACCGGTCCTTC+AGG	0.414102	8:-76484864	MsaT045739.1:CDS
CAATCTTCTGGTCCCTCAGC+TGG	0.414974	8:-76484903	MsaT045739.1:CDS
CAATCCTCTGGTCCCTCAGC+TGG	0.414974	8:-76484933	MsaT045739.1:CDS
TCTACATGGCTCATTTGTCC+AGG	0.415319	8:-76485213	MsaT045739.1:CDS
GCACCGACGGAAGTCCATTC+CGG	0.418324	8:+76486587	None:intergenic
AAAACGGGACAATGCAAGTT+TGG	0.420191	8:-76485413	MsaT045739.1:CDS
CCCACAGGATAGGGTGCAAC+TGG	0.421391	8:+76483476	None:intergenic
AGAGGTGAAACTTGGGGAAC+TGG	0.421536	8:+76485332	None:intergenic
ACCTGAAAGGCCTGATCAGC+AGG	0.421548	8:-76484817	MsaT045739.1:CDS
GCATTGGCAGAGGTGAAACT+TGG	0.425784	8:+76485324	None:intergenic
AGTCCGGGACATACCTGATA+AGG	0.428673	8:+76485134	None:intergenic
ATAGGACTAAGGTTCACTTT+TGG	0.429387	8:+76484714	None:intergenic
GACTTTGTCAACGGTTGTAC+CGG	0.430550	8:-76483318	MsaT045739.1:CDS
TATCCTGTGGGTTCCGCCAT+TGG	0.430848	8:-76483464	MsaT045739.1:CDS
CAATCTTCTGGTCCCTCAAC+CGG	0.435809	8:-76484873	MsaT045739.1:CDS
AGTTGGACAGCCCGTTTGCC+AGG	0.438645	8:-76486023	MsaT045739.1:intron
AAGCAGATGGACTGTAACTC+AGG	0.442386	8:+76483516	None:intergenic
GGGCGCAAAGGAAGGCCAAT+AGG	0.442531	8:+76484696	None:intergenic
GAATTAACCACCTGGCAAAC+GGG	0.443512	8:+76486013	None:intergenic
TGTATCTATTATTTGAGGAC+AGG	0.449948	8:-76486390	MsaT045739.1:CDS
GTGTTCCTATTATGTGAAAA+CGG	0.451501	8:-76485429	MsaT045739.1:CDS
TGGGCATCTGATTGAGAAAC+CGG	0.455710	8:+76483299	None:intergenic
GGGTATGGTGGATTGGTTGG+TGG	0.457920	8:-76486450	MsaT045739.1:CDS
AGCACATCGACTGCTTCAGC+TGG	0.458965	8:-76483344	MsaT045739.1:CDS
GGAAGTACAGGGCTTGCTGC+TGG	0.460193	8:+76485044	None:intergenic
GTAGTTCCCGCCGCCTGTCT+AGG	0.464948	8:+76485627	None:intergenic
GCGGTTATGGTTCTAGGTGT+CGG	0.465761	8:-76486362	MsaT045739.1:CDS
TGTTGCAGGGTATGGTGGAT+TGG	0.467100	8:-76486457	MsaT045739.1:intron
TATGGTGGATTGGTTGGTGG+TGG	0.468504	8:-76486447	MsaT045739.1:CDS
CACCCGCAGGCTGTGGATGA+TGG	0.471640	8:+76485375	None:intergenic
ATTTGGCCACCCGCAGGCTG+TGG	0.472332	8:+76485368	None:intergenic
CAGCTGAGGGACCAGAAGAT+TGG	0.481828	8:+76484904	None:intergenic
ACACTCAACGGAGTACTAGA+AGG	0.482108	8:+76485023	None:intergenic
GGTCCTTCACAACAACAATA+TGG	0.484846	8:-76485260	MsaT045739.1:CDS
AACTCGGATGATGAGGATGA+TGG	0.484942	8:+76483431	None:intergenic
TATTGGCCTTCCTTTGCGCC+CGG	0.486134	8:-76484694	MsaT045739.1:intron
TGTTCCTATTATGTGAAAAC+GGG	0.487218	8:-76485428	MsaT045739.1:CDS
TTGAGGCCTGAACTTGCATC+AGG	0.491757	8:-76483410	MsaT045739.1:CDS
ACATTACCATTGGCCCATAA+GGG	0.494713	8:+76485189	None:intergenic
GTGAATGTTGCAGGGTATGG+TGG	0.494891	8:-76486462	MsaT045739.1:intron
ACTGGGAGTTGCTAAAGAAC+CGG	0.495795	8:+76483259	None:intergenic
AGCTGGCTTGACTTTGTCAA+CGG	0.496678	8:-76483327	MsaT045739.1:CDS
TAGATACAATCAGCTTCATC+AGG	0.499288	8:+76486405	None:intergenic
CTACCTGAAGGACCGGTTGA+GGG	0.505161	8:+76484861	None:intergenic
ATAGGTGAAGGTACCGGCAT+TGG	0.508949	8:+76485308	None:intergenic
CTGGTGATGGTGCTATCATT+TGG	0.515476	8:+76485351	None:intergenic
GGAGTACTAGAAGGAAGTAC+AGG	0.516255	8:+76485032	None:intergenic
ATATCATCATCCGCCGGACA+TGG	0.516601	8:-76484742	MsaT045739.1:CDS
GCCAGTTGCACCCTATCCTG+TGG	0.516851	8:-76483477	MsaT045739.1:CDS
GAACCGGAACTCTGAGAAGA+TGG	0.517115	8:+76483275	None:intergenic
TCTAGTACTCCGTTGAGTGT+TGG	0.517479	8:-76485020	MsaT045739.1:CDS
CGGAACTCTGAGAAGATGGT+TGG	0.517807	8:+76483279	None:intergenic
TTCCATCATCCACAGCCTGC+GGG	0.519032	8:-76485377	MsaT045739.1:CDS
GCTATCATTTGGCCACCCGC+AGG	0.521595	8:+76485362	None:intergenic
ACTCTGATCCGTCGCCGGAA+TGG	0.523998	8:-76486601	MsaT045739.1:CDS
CAGCTGAGGGACCAGAGGAT+TGG	0.524806	8:+76484934	None:intergenic
CCATTGGCCCATAAGGGCCC+TGG	0.526364	8:+76485195	None:intergenic
GAAGGTACCGGCATTGGCAG+AGG	0.526800	8:+76485314	None:intergenic
CTACATGGCTCATTTGTCCA+GGG	0.527744	8:-76485212	MsaT045739.1:CDS
CTGCAGTACTATGCGAGGAC+GGG	0.528562	8:-76485687	MsaT045739.1:intron
GTGCTACTTGTGGTGGCACT+GGG	0.532061	8:+76483242	None:intergenic
ATGGCGGAACCCACAGGATA+GGG	0.532120	8:+76483467	None:intergenic
GGTCCAGTATAGACGGTTGT+TGG	0.532400	8:+76484960	None:intergenic
ACTCGGATGATGAGGATGAT+GGG	0.532572	8:+76483432	None:intergenic
CATTGGCAGAGGTGAAACTT+GGG	0.537765	8:+76485325	None:intergenic
CAAGTATCACCATCCTAGAC+AGG	0.538523	8:-76485640	MsaT045739.1:CDS
GTCCGGGACATACCTGATAA+GGG	0.540447	8:+76485135	None:intergenic
AATGGCGGAACCCACAGGAT+AGG	0.542826	8:+76483466	None:intergenic
TGCAACTGGCATATCAGAAA+GGG	0.543825	8:+76483490	None:intergenic
GGAACTCTGAGAAGATGGTT+GGG	0.544097	8:+76483280	None:intergenic
GCGAGGCCACCTCTTCTACA+TGG	0.546491	8:-76485227	MsaT045739.1:CDS
ACTGGACCTTACCAATCCTC+TGG	0.549245	8:-76484945	MsaT045739.1:CDS
GTACCAATGGCGGAACCCAC+AGG	0.549531	8:+76483461	None:intergenic
GTTGCACTCTGATCCGTCGC+CGG	0.554636	8:-76486606	MsaT045739.1:CDS
CATTCACATTGCTTAGCTTG+AGG	0.554771	8:+76483208	None:intergenic
CCGCGCCACGGTCACGTGGA+TGG	0.555477	8:+76486334	None:intergenic
TTCCCGCCGCCTGTCTAGGA+TGG	0.555701	8:+76485631	None:intergenic
TGCAAGTTTGATCATCCTAC+GGG	0.556067	8:-76483542	MsaT045739.1:CDS
ATATTGTTGTTGTGAAGGAC+CGG	0.556322	8:+76485262	None:intergenic
CCAGTTGCACCCTATCCTGT+GGG	0.556861	8:-76483476	MsaT045739.1:CDS
AACTCGTTCTGGATATTCAC+CGG	0.557101	8:+76486042	None:intergenic
GTGCAACTGGCATATCAGAA+AGG	0.557979	8:+76483489	None:intergenic
ACTACCTGAAGGACCGGTTG+AGG	0.558770	8:+76484860	None:intergenic
AAGGAAGGCCAATAGGACTA+AGG	0.560421	8:+76484703	None:intergenic
GAAACTTGGGGAACTGGTGA+TGG	0.560745	8:+76485338	None:intergenic
ACGGAAGTCCATTCCGGCGA+CGG	0.563671	8:+76486593	None:intergenic
GTATAGACGGTTGTTGGTGA+AGG	0.565761	8:+76484966	None:intergenic
TCATTACCGGGCGCAAAGGA+AGG	0.566968	8:+76484688	None:intergenic
GAGGGACCAGAAGATTGATA+AGG	0.567065	8:+76484879	None:intergenic
AGTTTCACCTCTGCCAATGC+CGG	0.567846	8:-76485321	MsaT045739.1:CDS
ACTTCCGTCGGTGCTGATGA+AGG	0.569648	8:-76486578	MsaT045739.1:intron
GCTGCAGTACTATGCGAGGA+CGG	0.572531	8:-76485688	MsaT045739.1:intron
GTGCAAGTTTGATCATCCTA+CGG	0.575614	8:-76483543	MsaT045739.1:CDS
ACACCATATTGTTGTTGTGA+AGG	0.575855	8:+76485257	None:intergenic
CAAACTGTACAGCCTCCGTC+CGG	0.577193	8:-76485281	MsaT045739.1:CDS
CGCTGGGTATAATGAGTGCA+AGG	0.577555	8:+76483590	None:intergenic
TCACCATCCTAGACAGGCGG+CGG	0.577651	8:-76485634	MsaT045739.1:CDS
CCAGGGCCCTTATGGGCCAA+TGG	0.578875	8:-76485195	MsaT045739.1:CDS
GAGACGACCCATCGTTAGCT+CGG	0.581397	8:+76486630	None:intergenic
ATAATCATTACCGGGCGCAA+AGG	0.584792	8:+76484684	None:intergenic
TATCATCATCCGCCGGACAT+GGG	0.589789	8:-76484741	MsaT045739.1:CDS
CTCGGATGATGAGGATGATG+GGG	0.590885	8:+76483433	None:intergenic
CTGACTACTACCTGAAGGAC+CGG	0.592024	8:+76484854	None:intergenic
AGGCCTCAACTCGGATGATG+AGG	0.592496	8:+76483424	None:intergenic
TTTACCGCGCCACGGTCACG+TGG	0.593039	8:+76486330	None:intergenic
TGCAAGTTCAGGCCTCAACT+CGG	0.595724	8:+76483415	None:intergenic
TCCTTCAGGTAGTAGTCAGA+AGG	0.595822	8:-76484850	MsaT045739.1:CDS
CACCATCCTAGACAGGCGGC+GGG	0.598008	8:-76485633	MsaT045739.1:CDS
AGATTGATAAGGACCAGCTG+AGG	0.601283	8:+76484890	None:intergenic
TCAGAAAGGGAAGAAGCAGA+TGG	0.603283	8:+76483503	None:intergenic
CATCCTCATCATCCGAGTTG+AGG	0.603884	8:-76483427	MsaT045739.1:CDS
GATGACATTCTGGATGAAGC+AGG	0.604175	8:+76483374	None:intergenic
AGATTGGTAAGGACCAGCTG+AGG	0.604660	8:+76484920	None:intergenic
TGATGGGGCAAGAGTACCAA+TGG	0.604718	8:+76483448	None:intergenic
ATTCCATCATCCACAGCCTG+CGG	0.605023	8:-76485378	MsaT045739.1:CDS
TTTGGTGCACCCATGTCCGG+CGG	0.607060	8:+76484732	None:intergenic
TCGCCGGAATGGACTTCCGT+CGG	0.607142	8:-76486590	MsaT045739.1:CDS
TGATAAATAGGTGAAGGTAC+CGG	0.608014	8:+76485302	None:intergenic
AAAGTACAAACCACTCGCAA+CGG	0.608625	8:+76485573	None:intergenic
AAACTTACAAAATCCACGCT+GGG	0.608759	8:+76483574	None:intergenic
TCAGCTTCATCAGGTCGTTG+TGG	0.608955	8:+76486414	None:intergenic
GAGGGACCAGAGGATTGGTA+AGG	0.609630	8:+76484939	None:intergenic
CCATAACAATTTAGCGACAA+GGG	0.610124	8:+76485600	None:intergenic
TGCGTCGAACCAACACTCAA+CGG	0.610522	8:+76485011	None:intergenic
TCCATAACAATTTAGCGACA+AGG	0.611754	8:+76485599	None:intergenic
GGTGAATATCCAGAACGAGT+TGG	0.612989	8:-76486040	MsaT045739.1:CDS
AGGTACGGCCGAGCTAACGA+TGG	0.613122	8:-76486638	MsaT045739.1:CDS
ACAAATGAGCCATGTAGAAG+AGG	0.616318	8:+76485218	None:intergenic
TTGGTAAGGTCCAGTATAGA+CGG	0.617115	8:+76484953	None:intergenic
GGTACGGCCGAGCTAACGAT+GGG	0.619629	8:-76486637	MsaT045739.1:CDS
GAGGGACCAGAAGATTGGTA+AGG	0.620595	8:+76484909	None:intergenic
AGCAGATGGACTGTAACTCA+GGG	0.621538	8:+76483517	None:intergenic
CATCATCCACAGCCTGCGGG+TGG	0.623790	8:-76485374	MsaT045739.1:CDS
CAAACTTACAAAATCCACGC+TGG	0.625616	8:+76483573	None:intergenic
AGTACCTTCATCAGCACCGA+CGG	0.628033	8:+76486574	None:intergenic
CCATCCACGTGACCGTGGCG+CGG	0.628156	8:-76486334	MsaT045739.1:intron
AATGAGCCATGTAGAAGAGG+TGG	0.628441	8:+76485221	None:intergenic
TTATGGATATCCGTTGCGAG+TGG	0.629078	8:-76485583	MsaT045739.1:intron
AGGAACACTCATTACCTGAA+AGG	0.629964	8:-76484830	MsaT045739.1:CDS
TCCTTCTGACTACTACCTGA+AGG	0.631642	8:+76484849	None:intergenic
GATTGATAAGGACCAGCTGA+GGG	0.633302	8:+76484891	None:intergenic
ACAGTTTGATAAATAGGTGA+AGG	0.633435	8:+76485296	None:intergenic
GTGAGAGACGGTGCTACTTG+TGG	0.638791	8:+76483232	None:intergenic
TTTAACCATCCACGTGACCG+TGG	0.642316	8:-76486339	MsaT045739.1:CDS
GTCAGCATTACATGAAAACA+GGG	0.642817	8:-76484791	MsaT045739.1:CDS
GATTGGTAAGGACCAGCTGA+GGG	0.643343	8:+76484921	None:intergenic
TTAGCTTGAGGTGTGAGAGA+CGG	0.646603	8:+76483220	None:intergenic
AGGACCAGCTGAGGGACCAG+AGG	0.646769	8:+76484929	None:intergenic
TGCACTCATTATACCCAGCG+TGG	0.650258	8:-76483587	MsaT045739.1:CDS
TGTTGTTGTGAAGGACCGGA+CGG	0.652804	8:+76485266	None:intergenic
ATGTAGATATCATCATCCGC+CGG	0.657903	8:-76484748	MsaT045739.1:CDS
AATATGGTGTACTTGTTGCG+AGG	0.663439	8:-76485244	MsaT045739.1:CDS
TCATGCTGCAGTACTATGCG+AGG	0.670087	8:-76485692	MsaT045739.1:intron
GAGTACTAGAAGGAAGTACA+GGG	0.672699	8:+76485033	None:intergenic
CGTACAATGGAACAAAGGTA+CGG	0.672974	8:-76486653	None:intergenic
ATTGGCAGAGGTGAAACTTG+GGG	0.673579	8:+76485326	None:intergenic
ACTTGCATCAGGATCCAGCA+AGG	0.675326	8:-76483399	MsaT045739.1:CDS
TCGTCCGTACAATGGAACAA+AGG	0.678724	8:-76486658	None:intergenic
CTGTAACTCAGGGATCCCGT+AGG	0.679795	8:+76483527	None:intergenic
GTATCACCATCCTAGACAGG+CGG	0.680328	8:-76485637	MsaT045739.1:CDS
TTTAGGTTATTGGAGCTGCG+AGG	0.685804	8:-76486069	MsaT045739.1:intron
AGAGACGGTGCTACTTGTGG+TGG	0.702854	8:+76483235	None:intergenic
TGTTGTGAAGGACCGGACGG+AGG	0.705461	8:+76485269	None:intergenic
TGGGGCAAGAGTACCAATGG+CGG	0.727761	8:+76483451	None:intergenic
TCAGCATTACATGAAAACAG+GGG	0.793025	8:-76484790	MsaT045739.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	76483220	76486669	76483220	ID=MsaG045739
Chr8	mRNA	76483220	76486669	76483220	ID=MsaT045739.1;Parent=MsaG045739
Chr8	exon	76483220	76483621	76483220	ID=MsaT045739.1.exon7;Parent=MsaT045739.1
Chr8	CDS	76483220	76483621	76483220	ID=cds.MsaT045739.1;Parent=MsaT045739.1
Chr8	exon	76484695	76485069	76484695	ID=MsaT045739.1.exon6;Parent=MsaT045739.1
Chr8	CDS	76484695	76485069	76484695	ID=cds.MsaT045739.1;Parent=MsaT045739.1
Chr8	exon	76485148	76485462	76485148	ID=MsaT045739.1.exon5;Parent=MsaT045739.1
Chr8	CDS	76485148	76485462	76485148	ID=cds.MsaT045739.1;Parent=MsaT045739.1
Chr8	exon	76485584	76485703	76485584	ID=MsaT045739.1.exon4;Parent=MsaT045739.1
Chr8	CDS	76485584	76485703	76485584	ID=cds.MsaT045739.1;Parent=MsaT045739.1
Chr8	exon	76486024	76486086	76486024	ID=MsaT045739.1.exon3;Parent=MsaT045739.1
Chr8	CDS	76486024	76486086	76486024	ID=cds.MsaT045739.1;Parent=MsaT045739.1
Chr8	exon	76486335	76486471	76486335	ID=MsaT045739.1.exon2;Parent=MsaT045739.1
Chr8	CDS	76486335	76486471	76486335	ID=cds.MsaT045739.1;Parent=MsaT045739.1
Chr8	exon	76486579	76486669	76486579	ID=MsaT045739.1.exon1;Parent=MsaT045739.1
Chr8	CDS	76486579	76486669	76486579	ID=cds.MsaT045739.1;Parent=MsaT045739.1

Gene Sequence

>MsaT045739.1

ATGGAACAAAGGTACGGCCGAGCTAACGATGGGTCGTCTCAGTTGCACTCTGATCCGTCGCCGGAATGGACTTCCGTCGGTGCTGATGAAGGGTATGGTGGATTGGTTGGTGGTGGTGAATCGTATCCACAACGACCTGATGAAGCTGATTGTATCTATTATTTGAGGACAGGTTTTTGCGGTTATGGTTCTAGGTGTCGGTTTAACCATCCACGTGACCGTGGCGCGGTTATTGGAGCTGCGAGGATTGCCGGTGAATATCCAGAACGAGTTGGACAGCCCGTTTGCCAGTACTATGCGAGGACGGGATCGTGCAAATTTGGCGCTTCCTGCAAGTATCACCATCCTAGACAGGCGGCGGGAACTACTGCTCCCTTGTCGCTAAATTGTTATGGATATCCGTTGCGAGTGGGTGAGAAAGAGTGTTCCTATTATGTGAAAACGGGACAATGCAAGTTTGGTGCAACATGTAAATTCCATCATCCACAGCCTGCGGGTGGCCAAATGATAGCACCATCACCAGTTCCCCAAGTTTCACCTCTGCCAATGCCGGTACCTTCACCTATTTATCAAACTGTACAGCCTCCGTCCGGTCCTTCACAACAACAATATGGTGTACTTGTTGCGAGGCCACCTCTTCTACATGGCTCATTTGTCCAGGGCCCTTATGGGCCAATGGTAATGTCCCCCACCATGGTCCCTTTTTCAGGCTGGAGCCCTTATCAGGCTCCAGCAGCAAGCCCTGTACTTCCTTCTAGTACTCCGTTGAGTGTTGGTTCGACGCAGCTTTATGGGATAACTCAGCTACCTTCACCAACAACCGTCTATACTGGACCTTACCAATCCTCTGGTCCCTCAGCTGGTCCTTACCAATCTTCTGGTCCCTCAGCTGGTCCTTATCAATCTTCTGGTCCCTCAACCGGTCCTTCAGGTAGTAGTCAGAAGGAACACTCATTACCTGAAAGGCCTGATCAGCAGGAATGTCAGCATTACATGAAAACAGGGGACTGTAAATTTGGTTCTACATGTAGATATCATCATCCGCCGGACATGGGTGCACCAAAAGTGAACCTTAGTCCTATTGGCCTTCCTTTGCGCCCGGGAGCACAACCTTGCACTCATTATACCCAGCGTGGATTTTGTAAGTTTGGATCTGCGTGCAAGTTTGATCATCCTACGGGATCCCTGAGTTACAGTCCATCTGCTTCTTCCCTTTCTGATATGCCAGTTGCACCCTATCCTGTGGGTTCCGCCATTGGTACTCTTGCCCCATCATCCTCATCATCCGAGTTGAGGCCTGAACTTGCATCAGGATCCAGCAAGGAGCCTGCTTCATCCAGAATGTCATCATCAATGAGCACATCGACTGCTTCAGCTGGCTTGACTTTGTCAACGGTTGTACCGGTTTCTCAATCAGATGCCCAACCATCTTCTCAGAGTTCCGGTTCTTTAGCAACTCCCAGTGCCACCACAAGTAGCACCGTCTCTCACACCTCAAGCTAA

Protein sequence

>MsaT045739.1

MEQRYGRANDGSSQLHSDPSPEWTSVGADEGYGGLVGGGESYPQRPDEADCIYYLRTGFCGYGSRCRFNHPRDRGAVIGAARIAGEYPERVGQPVCQYYARTGSCKFGASCKYHHPRQAAGTTAPLSLNCYGYPLRVGEKECSYYVKTGQCKFGATCKFHHPQPAGGQMIAPSPVPQVSPLPMPVPSPIYQTVQPPSGPSQQQYGVLVARPPLLHGSFVQGPYGPMVMSPTMVPFSGWSPYQAPAASPVLPSSTPLSVGSTQLYGITQLPSPTTVYTGPYQSSGPSAGPYQSSGPSAGPYQSSGPSTGPSGSSQKEHSLPERPDQQECQHYMKTGDCKFGSTCRYHHPPDMGAPKVNLSPIGLPLRPGAQPCTHYTQRGFCKFGSACKFDHPTGSLSYSPSASSLSDMPVAPYPVGSAIGTLAPSSSSSELRPELASGSSKEPASSRMSSSMSTSTASAGLTLSTVVPVSQSDAQPSSQSSGSLATPSATTSSTVSHTSS*