AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045758


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045758 MtrunA17_Chr4g0067121 81.301 246 19 1 26 244 147 392 4.69e-144 408
MsaG045758 MtrunA17_Chr2g0295731 46.639 238 100 1 26 236 144 381 9.40e-70 219
MsaG045758 MtrunA17_Chr2g0296151 43.284 268 123 2 4 242 113 380 1.56e-68 216
MsaG045758 MtrunA17_Chr2g0295741 44.813 241 106 1 28 241 145 385 3.69e-68 215
MsaG045758 MtrunA17_Chr2g0296111 48.133 241 95 3 31 242 141 380 1.45e-67 213
MsaG045758 MtrunA17_Chr2g0296121 44.262 244 109 1 26 242 223 466 3.67e-65 209
MsaG045758 MtrunA17_Chr2g0296291 45.417 240 101 2 31 240 167 406 3.71e-63 202
MsaG045758 MtrunA17_Chr2g0296251 43.038 237 105 2 26 232 162 398 3.36e-61 197
MsaG045758 MtrunA17_Chr2g0296741 40.000 275 127 4 1 240 140 411 1.75e-60 196
MsaG045758 MtrunA17_Chr2g0296231 43.651 252 110 3 19 240 139 388 3.86e-60 194
MsaG045758 MtrunA17_Chr2g0296721 40.741 270 129 4 4 242 123 392 1.11e-59 193
MsaG045758 MtrunA17_Chr2g0296191 44.961 258 111 3 4 231 253 509 2.87e-59 201
MsaG045758 MtrunA17_Chr2g0296191 40.851 235 106 4 28 229 760 994 1.82e-48 171
MsaG045758 MtrunA17_Chr2g0296711 40.364 275 130 5 1 242 123 396 2.35e-58 191
MsaG045758 MtrunA17_Chr2g0315731 40.520 269 128 4 4 240 143 411 2.03e-57 188
MsaG045758 MtrunA17_Chr2g0295761 39.544 263 127 4 4 235 131 392 9.02e-57 186
MsaG045758 MtrunA17_Chr2g0296181 43.320 247 131 6 1 240 121 365 1.09e-56 185
MsaG045758 MtrunA17_Chr4g0067141 39.922 258 122 4 4 230 115 370 1.11e-56 185
MsaG045758 MtrunA17_Chr2g0296221 42.276 246 109 4 28 240 177 422 1.47e-56 186
MsaG045758 MtrunA17_Chr2g0296171 42.437 238 104 4 28 232 158 395 1.45e-53 177
MsaG045758 MtrunA17_Chr2g0296551 42.708 192 83 1 76 240 1 192 2.68e-49 160
MsaG045758 MtrunA17_Chr2g0296301 34.855 241 128 2 26 237 131 371 9.98e-49 164
MsaG045758 MtrunA17_Chr2g0296161 45.238 168 65 1 102 242 1 168 1.40e-48 157
MsaG045758 MtrunA17_Chr2g0296211 39.035 228 96 2 58 242 141 368 3.19e-48 163
MsaG045758 MtrunA17_Chr2g0296131 43.860 171 69 1 99 242 2 172 1.60e-47 155
MsaG045758 MtrunA17_Chr2g0296701 47.239 163 59 1 105 240 2 164 1.00e-44 148
MsaG045758 MtrunA17_Chr4g0067671 35.391 243 126 3 4 216 115 356 1.12e-44 153
MsaG045758 MtrunA17_Chr2g0286191 36.398 261 131 6 4 232 102 359 2.46e-43 150
MsaG045758 MtrunA17_Chr2g0296751 49.231 130 39 1 136 238 6 135 4.27e-33 118
MsaG045758 MtrunA17_Chr2g0286221 38.202 178 73 5 92 241 10 178 1.33e-31 114
MsaG045758 MtrunA17_Chr2g0295721 55.556 81 36 0 102 182 1 81 6.49e-28 103
MsaG045758 MtrunA17_Chr4g0001831 27.170 265 151 6 4 230 108 368 5.79e-21 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045758 AT5G07900.1 29.389 262 148 7 4 229 139 399 3.46e-31 119
MsaG045758 AT1G21150.3 24.335 263 162 6 3 229 89 350 1.98e-12 66.2
MsaG045758 AT1G21150.1 24.151 265 164 6 1 229 123 386 2.58e-12 66.2
MsaG045758 AT1G21150.2 24.060 266 159 6 1 227 187 448 2.95e-12 66.2

Find 44 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAACGATTAATACATTTAA+TGG 0.168353 8:-76696452 None:intergenic
TATGACAGAGAAGTTGTTTC+TGG 0.244337 8:+76696986 MsaT045758.1:CDS
GTCAAATTATTGCTTGATAA+TGG 0.274159 8:+76696511 MsaT045758.1:CDS
TCATTTGTTGAAGCTGTATC+AGG 0.289926 8:+76697043 MsaT045758.1:CDS
AACACATTCTTCAAGCATTT+AGG 0.305458 8:+76696746 MsaT045758.1:CDS
TTTGGTAAAAGTACCTTGTT+GGG 0.357862 8:-76696280 None:intergenic
GATTGGGCAAACTTCTTAAG+AGG 0.387256 8:-76696375 None:intergenic
CAACCTGTTGGAGACTGTTC+AGG 0.388932 8:+76696594 MsaT045758.1:CDS
CTTTGGTAAAAGTACCTTGT+TGG 0.403823 8:-76696281 None:intergenic
ACCTTACTGTATGTTGTCTT+CGG 0.415933 8:+76696774 MsaT045758.1:CDS
TTGCAGTTCCTTGTATCTAA+AGG 0.428240 8:+76696924 MsaT045758.1:CDS
AAATTCGTAATATCATCAAA+AGG 0.437298 8:+76696230 MsaT045758.1:CDS
AGCGCTTGGGTTGATCAGTT+AGG 0.440546 8:+76696820 MsaT045758.1:CDS
ATAAGTAGGGATGATATGAT+TGG 0.445105 8:-76696392 None:intergenic
ATTCCTGAACAGTCTCCAAC+AGG 0.475079 8:-76696597 None:intergenic
TTCTCAAACCTTTAGATACA+AGG 0.476085 8:-76696932 None:intergenic
CCTCTCATAAAATGATAAGT+AGG 0.495753 8:-76696406 None:intergenic
AACTTCTCTGTCATAGCAAA+CGG 0.496374 8:-76696978 None:intergenic
AAAGTACCTTGTTGGGGTCG+AGG 0.506736 8:-76696273 None:intergenic
AATTCGTAATATCATCAAAA+GGG 0.515448 8:+76696231 MsaT045758.1:CDS
TTGGATGAACAATATATCCT+AGG 0.518394 8:-76696574 None:intergenic
TAAGTAGGGATGATATGATT+GGG 0.531463 8:-76696391 None:intergenic
ATATTGTTCATCCAACCTGT+TGG 0.532063 8:+76696582 MsaT045758.1:CDS
GATATTACGAATTTGAGTGT+TGG 0.541490 8:-76696221 None:intergenic
ACATTCTTCAAGCATTTAGG+AGG 0.543592 8:+76696749 MsaT045758.1:CDS
AATGAATCTTGCAAATAGCA+TGG 0.553137 8:+76697070 MsaT045758.1:CDS
CTCTCATAAAATGATAAGTA+GGG 0.556083 8:-76696405 None:intergenic
GTTCAGGAATTGAAGCAACT+AGG 0.561440 8:+76696610 MsaT045758.1:CDS
AGAAGTTACACACTTCATGA+AGG 0.561506 8:-76697101 None:intergenic
TGATACAGCTTCAACAAATG+AGG 0.568471 8:-76697041 None:intergenic
CATTGTTGCGTAATTCGCCT+AGG 0.579851 8:+76696557 MsaT045758.1:CDS
AGAAAACTGTGAATGAAACT+AGG 0.580330 8:+76696677 MsaT045758.1:CDS
CTGAACAGTCTCCAACAGGT+TGG 0.584404 8:-76696593 None:intergenic
GCCGAAGACAACATACAGTA+AGG 0.592002 8:-76696775 None:intergenic
TTTGAGTGTTGGAGAAGCCA+TGG 0.596210 8:-76696210 None:intergenic
AGCAAACGGTGCACTTAGTG+AGG 0.600976 8:-76696964 None:intergenic
AACTGTGAATGAAACTAGGT+GGG 0.613433 8:+76696681 MsaT045758.1:CDS
TTGAGTGTTGGAGAAGCCAT+GGG 0.633074 8:-76696209 None:intergenic
CTTATGCCTCGACCCCAACA+AGG 0.635453 8:+76696267 MsaT045758.1:CDS
TGTAGCAATATTTGAGTGTG+TGG 0.635496 8:-76696536 None:intergenic
AAACTGTGAATGAAACTAGG+TGG 0.639268 8:+76696680 MsaT045758.1:CDS
ATATCATCAAAAGGGAACCT+CGG 0.640196 8:+76696239 MsaT045758.1:CDS
TTGGTAAAAGTACCTTGTTG+GGG 0.646951 8:-76696279 None:intergenic
TCGAGGCATAAGAGTAACCG+AGG 0.694428 8:-76696256 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 76696212 76697109 76696212 ID=MsaG045758
Chr8 mRNA 76696212 76697109 76696212 ID=MsaT045758.1;Parent=MsaG045758
Chr8 exon 76696212 76696288 76696212 ID=MsaT045758.1.exon1;Parent=MsaT045758.1
Chr8 CDS 76696212 76696288 76696212 ID=cds.MsaT045758.1;Parent=MsaT045758.1
Chr8 exon 76696372 76696841 76696372 ID=MsaT045758.1.exon2;Parent=MsaT045758.1
Chr8 CDS 76696372 76696841 76696372 ID=cds.MsaT045758.1;Parent=MsaT045758.1
Chr8 exon 76696922 76697109 76696922 ID=MsaT045758.1.exon3;Parent=MsaT045758.1
Chr8 CDS 76696922 76697109 76696922 ID=cds.MsaT045758.1;Parent=MsaT045758.1
Gene Sequence

>MsaT045758.1

ATGGCTTCTCCAACACTCAAATTCGTAATATCATCAAAAGGGAACCTCGGTTACTCTTATGCCTCGACCCCAACAAGATTCCTCTTAAGAAGTTTGCCCAATCATATCATCCCTACTTATCATTTTATGAGAGGGTTTCTTAAATCTGATAAACAAACCATTAAATGTATTAATCGTTATGCTTCTTTTATCTCTGATTCTCGTGTCGAAAGTAATGTCAAATTATTGCTTGATAATGGAGCCACACACTCAAATATTGCTACATTGTTGCGTAATTCGCCTAGGATATATTGTTCATCCAACCTGTTGGAGACTGTTCAGGAATTGAAGCAACTAGGTTTCAATTCTTCAACTTCAACTTTTGCTGTTGCATTGGTTGCTAAGAAAACTGTGAATGAAACTAGGTGGGCTGAGAAAGTTGAAATTTTTAAGAAATGGGGTTGGTCGGATGAACACATTCTTCAAGCATTTAGGAGGCAACCTTACTGTATGTTGTCTTCGGCTCAAAAAATCGATGCAGTTTTGAGCGCTTGGGTTGATCAGTTAGTTTTGCAGTTCCTTGTATCTAAAGGTTTGAGAAGAAAGAACGCCTCACTAAGTGCACCGTTTGCTATGACAGAGAAGTTGTTTCTGGACAAATTTGTGAAATGTTTTAAGGAGGATTCTCCTCATTTGTTGAAGCTGTATCAGGAAAAAATGAATCTTGCAAATAGCATGGAGAAGAGTCCTTCATGA

Protein sequence

>MsaT045758.1

MASPTLKFVISSKGNLGYSYASTPTRFLLRSLPNHIIPTYHFMRGFLKSDKQTIKCINRYASFISDSRVESNVKLLLDNGATHSNIATLLRNSPRIYCSSNLLETVQELKQLGFNSSTSTFAVALVAKKTVNETRWAEKVEIFKKWGWSDEHILQAFRRQPYCMLSSAQKIDAVLSAWVDQLVLQFLVSKGLRRKNASLSAPFAMTEKLFLDKFVKCFKEDSPHLLKLYQEKMNLANSMEKSPS*