AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045759


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045759 MtrunA17_Chr4g0067671 62.598 254 50 1 1 209 104 357 1.53e-106 311
MsaG045759 MtrunA17_Chr4g0067141 56.506 269 72 1 1 224 104 372 2.44e-93 278
MsaG045759 MtrunA17_Chr2g0295761 40.076 262 111 1 5 220 124 385 3.35e-58 188
MsaG045759 MtrunA17_Chr4g0067121 39.924 263 113 1 5 222 116 378 2.14e-57 186
MsaG045759 MtrunA17_Chr4g0067131 91.860 86 7 0 140 225 1 86 4.79e-54 168
MsaG045759 MtrunA17_Chr2g0296301 37.692 260 115 2 8 220 103 362 6.66e-54 177
MsaG045759 MtrunA17_Chr2g0295731 34.444 270 132 1 1 225 109 378 1.24e-50 169
MsaG045759 MtrunA17_Chr2g0296191 37.407 270 124 1 1 225 242 511 4.18e-50 174
MsaG045759 MtrunA17_Chr2g0296191 36.431 269 121 5 1 219 724 992 5.05e-44 157
MsaG045759 MtrunA17_Chr2g0296721 36.704 267 122 2 1 220 112 378 8.02e-50 167
MsaG045759 MtrunA17_Chr2g0296741 36.567 268 122 3 1 220 132 399 2.39e-49 166
MsaG045759 MtrunA17_Chr2g0296171 37.407 270 119 4 1 220 122 391 2.88e-49 166
MsaG045759 MtrunA17_Chr2g0296151 35.581 267 127 1 1 222 102 368 2.54e-48 162
MsaG045759 MtrunA17_Chr2g0296121 35.688 269 128 1 1 224 188 456 4.73e-48 164
MsaG045759 MtrunA17_Chr2g0296251 36.434 258 116 3 13 222 139 396 1.87e-47 160
MsaG045759 MtrunA17_Chr2g0315731 34.926 272 121 3 1 220 132 399 2.21e-47 161
MsaG045759 MtrunA17_Chr2g0296231 36.882 263 116 3 6 220 116 376 2.92e-47 160
MsaG045759 MtrunA17_Chr2g0296111 36.704 267 121 3 1 220 101 366 1.04e-46 158
MsaG045759 MtrunA17_Chr2g0296711 35.448 268 125 3 1 220 115 382 1.28e-46 159
MsaG045759 MtrunA17_Chr2g0295741 34.082 267 131 1 1 222 108 374 6.99e-46 156
MsaG045759 MtrunA17_Chr2g0296221 36.029 272 124 5 1 222 141 412 1.95e-45 156
MsaG045759 MtrunA17_Chr2g0296291 35.145 276 125 4 1 222 121 396 5.46e-44 152
MsaG045759 MtrunA17_Chr2g0286191 33.829 269 121 4 5 222 95 357 3.69e-40 141
MsaG045759 MtrunA17_Chr2g0296181 35.366 246 128 7 1 220 113 353 5.29e-36 130
MsaG045759 MtrunA17_Chr2g0296131 50.467 107 52 1 117 222 54 160 4.71e-32 115
MsaG045759 MtrunA17_Chr2g0296551 35.714 182 72 1 86 222 1 182 6.72e-30 110
MsaG045759 MtrunA17_Chr2g0296161 53.535 99 46 0 124 222 58 156 7.68e-30 109
MsaG045759 MtrunA17_Chr2g0296751 50.000 98 49 0 125 222 30 127 9.52e-28 103
MsaG045759 MtrunA17_Chr2g0296701 50.000 98 49 0 125 222 57 154 1.14e-27 103
MsaG045759 MtrunA17_Chr2g0296211 35.294 170 65 1 98 222 187 356 4.27e-27 106
MsaG045759 MtrunA17_Chr4g0067681 66.102 59 20 0 129 187 1 59 8.31e-22 85.1
MsaG045759 MtrunA17_Chr2g0286221 31.481 162 99 2 72 222 7 167 2.32e-19 82.0
MsaG045759 MtrunA17_Chr4g0001831 26.022 269 147 4 1 218 97 364 2.65e-19 85.1
MsaG045759 MtrunA17_Chr2g0295721 47.458 59 31 0 124 182 58 116 3.94e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045759 AT5G07900.1 22.263 274 162 3 1 223 128 401 6.02e-18 82.0
MsaG045759 AT1G61990.2 30.323 155 92 3 74 212 245 399 3.85e-13 68.2
MsaG045759 AT1G61990.1 30.323 155 92 3 74 212 245 399 3.85e-13 68.2
MsaG045759 AT1G61960.1 27.723 202 122 4 39 224 261 454 6.72e-13 67.4
MsaG045759 AT1G61980.3 26.744 172 111 4 55 212 234 404 7.41e-12 64.3
MsaG045759 AT1G61980.2 26.744 172 111 4 55 212 234 404 7.41e-12 64.3
MsaG045759 AT1G61980.1 26.744 172 111 4 55 212 234 404 7.41e-12 64.3
MsaG045759 AT3G46950.1 24.091 220 140 6 8 223 251 447 7.25e-11 61.6
MsaG045759 AT1G62085.1 23.923 209 131 5 8 212 260 444 9.36e-11 61.2
MsaG045759 AT1G62085.2 23.923 209 131 5 8 212 260 444 9.36e-11 61.2

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGGAGATTAAGGAATT+AGG 0.141632 8:-76701866 MsaT045759.1:CDS
TCAATGCAGTGATGAGATTT+TGG 0.150309 8:-76701668 MsaT045759.1:CDS
AAAGGCTTTGGAGGAGATTA+AGG 0.223358 8:-76701873 MsaT045759.1:CDS
ATGTACTAGTGATTTGAAAA+AGG 0.285250 8:-76701891 MsaT045759.1:CDS
TAAGCTATTAAAGCTAAATC+AGG 0.322367 8:-76701430 MsaT045759.1:CDS
TGGAGGAGATTAAGGAATTA+GGG 0.324678 8:-76701865 MsaT045759.1:intron
ACCCTTCTTCCTCAAACCTT+TGG 0.335743 8:+76701533 None:intergenic
CTTTGGCAAAATAGATTTGA+TGG 0.364807 8:+76702195 None:intergenic
ATCACTGCATTGAGTTTGTC+TGG 0.367766 8:+76701678 None:intergenic
GTCTTGGGGAGAAGCACCTT+TGG 0.383783 8:+76702156 None:intergenic
CAAAATAGATTTGATGGGGT+TGG 0.387430 8:+76702201 None:intergenic
TCCTCATCAGTTGCATGAAA+TGG 0.388426 8:+76701495 None:intergenic
GATGGCGGCAACAATGTCTT+GGG 0.431106 8:+76702141 None:intergenic
TTTCATGCGAGGATCGCTAA+TGG 0.434701 8:+76701994 None:intergenic
ATCCAAAGGTTTGAGGAAGA+AGG 0.441730 8:-76701535 MsaT045759.1:CDS
AACGTTTCACATACCTTCGC+TGG 0.459558 8:+76701469 None:intergenic
ATCTTCTATCCAAAGGTTTG+AGG 0.469136 8:-76701542 MsaT045759.1:CDS
TTGGGTATAGTATAGAGAAA+AGG 0.471370 8:-76701593 MsaT045759.1:CDS
ACGAACAAAACGAGGGCTTC+TGG 0.481817 8:+76702117 None:intergenic
TCACTAGTACATATTATAGA+AGG 0.488452 8:+76701902 None:intergenic
TGATGGCGGCAACAATGTCT+TGG 0.490931 8:+76702140 None:intergenic
TTTGGCAAAATAGATTTGAT+GGG 0.496666 8:+76702196 None:intergenic
TTGGGGAGAAGCACCTTTGG+AGG 0.496835 8:+76702159 None:intergenic
AAGCAGGGATAATGTGTTTG+TGG 0.497547 8:+76702086 None:intergenic
TCCAAAGGTTTGAGGAAGAA+GGG 0.500079 8:-76701534 MsaT045759.1:CDS
ATCAAGTATCTTCTATCCAA+AGG 0.502207 8:-76701549 MsaT045759.1:CDS
TGGAGACCGTAACATAAAGT+TGG 0.507075 8:+76701698 None:intergenic
CTTCTTACGAATTCAAAAGC+AGG 0.511076 8:+76702070 None:intergenic
TACTTGATAATTGATGCCCT+TGG 0.511956 8:+76701564 None:intergenic
TTGGGTCGATGAGCTTGGGT+GGG 0.521660 8:-76701649 MsaT045759.1:CDS
AAAACGAGGGCTTCTGGTGA+TGG 0.524399 8:+76702123 None:intergenic
TTTGGGTCGATGAGCTTGGG+TGG 0.525074 8:-76701650 MsaT045759.1:CDS
GGAGACCGTAACATAAAGTT+GGG 0.535336 8:+76701699 None:intergenic
AGCAACCCAACTTTATGTTA+CGG 0.535359 8:-76701704 MsaT045759.1:CDS
AAGGTCTGCTAGTAAGCAAA+CGG 0.543418 8:+76701921 None:intergenic
CGAGGATCGCTAATGGAAGT+AGG 0.552146 8:+76702001 None:intergenic
TAGAGAAAAGGCTTATTCCA+AGG 0.554127 8:-76701581 MsaT045759.1:CDS
TTCTTACGAATTCAAAAGCA+GGG 0.571751 8:+76702071 None:intergenic
CAGGGATAATGTGTTTGTGG+AGG 0.574406 8:+76702089 None:intergenic
TTAACATTTGGTTTCATGCG+AGG 0.580572 8:+76701983 None:intergenic
AAGCTATTAAAGCTAAATCA+GGG 0.584569 8:-76701429 MsaT045759.1:CDS
ACGAGGGCTTCTGGTGATGG+CGG 0.598934 8:+76702126 None:intergenic
ACCATTTCATGCAACTGATG+AGG 0.600954 8:-76701496 MsaT045759.1:CDS
TTGGCAAAATAGATTTGATG+GGG 0.611912 8:+76702197 None:intergenic
AGAGAAAAGGCTTATTCCAA+GGG 0.614046 8:-76701580 MsaT045759.1:CDS
AGGTTTACACGAACAAAACG+AGG 0.633789 8:+76702109 None:intergenic
CTGATGAGGATTTCCAGCGA+AGG 0.666538 8:-76701482 MsaT045759.1:CDS
ATGGCGGCAACAATGTCTTG+GGG 0.673173 8:+76702142 None:intergenic
GGTTTACACGAACAAAACGA+GGG 0.713136 8:+76702110 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 76701419 76702233 76701419 ID=MsaG045759
Chr8 mRNA 76701419 76702233 76701419 ID=MsaT045759.1;Parent=MsaG045759
Chr8 exon 76701419 76701728 76701419 ID=MsaT045759.1.exon2;Parent=MsaT045759.1
Chr8 CDS 76701419 76701728 76701419 ID=cds.MsaT045759.1;Parent=MsaT045759.1
Chr8 exon 76701866 76702233 76701866 ID=MsaT045759.1.exon1;Parent=MsaT045759.1
Chr8 CDS 76701866 76702233 76701866 ID=cds.MsaT045759.1;Parent=MsaT045759.1
Gene Sequence

>MsaT045759.1

ATGATCGTTTCCAACCCCATCAAATCTATTTTGCCAAAGATTCAATTTTTATCCTCCAAAGGTGCTTCTCCCCAAGACATTGTTGCCGCCATCACCAGAAGCCCTCGTTTTGTTCGTGTAAACCTCCACAAACACATTATCCCTGCTTTTGAATTCGTAAGAAGTTTCTGCCCCTCTGATCAAAAAGCTATTCATTCTATAATCACTTGTCCTACTTCCATTAGCGATCCTCGCATGAAACCAAATGTTAAATTTTTGCTTGATTTTGGTCTCACTGCTTCAAGTATTTACCGTTTGCTTACTAGCAGACCTTCTATAATATGTACTAGTGATTTGAAAAAGGCTTTGGAGGAGATTAAGGAATTAGGGAAGCAACCCAACTTTATGTTACGGTCTCCAGACAAACTCAATGCAGTGATGAGATTTTGGGTCGATGAGCTTGGGTGGGATGCTTTGCTGCTCTTAACTAAACCAGCAATTTTTGGGTATAGTATAGAGAAAAGGCTTATTCCAAGGGCATCAATTATCAAGTATCTTCTATCCAAAGGTTTGAGGAAGAAGGGTTTTAGCTTATGCACACCATTTCATGCAACTGATGAGGATTTCCAGCGAAGGTATGTGAAACGTTTTGAGGAGGACACATCTAAGCTATTAAAGCTAAATCAGGGAGAGTGCTAG

Protein sequence

>MsaT045759.1

MIVSNPIKSILPKIQFLSSKGASPQDIVAAITRSPRFVRVNLHKHIIPAFEFVRSFCPSDQKAIHSIITCPTSISDPRMKPNVKFLLDFGLTASSIYRLLTSRPSIICTSDLKKALEEIKELGKQPNFMLRSPDKLNAVMRFWVDELGWDALLLLTKPAIFGYSIEKRLIPRASIIKYLLSKGLRKKGFSLCTPFHATDEDFQRRYVKRFEEDTSKLLKLNQGEC*