AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045761

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045761 MtrunA17_Chr4g0067141 68.766 381 106 3 1 372 1 377 3.17e-177 497
MsaG045761 MtrunA17_Chr4g0067671 66.947 357 113 3 1 352 1 357 1.61e-171 482
MsaG045761 MtrunA17_Chr4g0067121 49.541 327 164 1 39 364 51 377 1.05e-107 321
MsaG045761 MtrunA17_Chr2g0295731 46.108 334 178 1 33 364 41 374 1.04e-103 311
MsaG045761 MtrunA17_Chr2g0295761 46.591 352 187 1 15 365 36 387 6.44e-103 309
MsaG045761 MtrunA17_Chr2g0296301 44.970 338 182 2 29 364 28 363 8.23e-102 306
MsaG045761 MtrunA17_Chr2g0296721 46.552 348 178 3 25 364 32 379 1.03e-100 304
MsaG045761 MtrunA17_Chr2g0296121 44.800 375 196 3 1 364 79 453 2.10e-100 305
MsaG045761 MtrunA17_Chr2g0296741 46.176 340 178 2 30 364 61 400 1.67e-99 301
MsaG045761 MtrunA17_Chr2g0315731 45.559 349 181 3 25 364 52 400 8.85e-99 300
MsaG045761 MtrunA17_Chr2g0296251 43.583 374 171 3 29 364 24 395 2.92e-96 292
MsaG045761 MtrunA17_Chr2g0296221 46.939 343 175 3 29 364 69 411 3.74e-96 293
MsaG045761 MtrunA17_Chr2g0296171 48.036 331 165 3 41 364 62 392 5.39e-96 292
MsaG045761 MtrunA17_Chr2g0296711 45.593 329 174 2 41 364 55 383 9.87e-95 289
MsaG045761 MtrunA17_Chr2g0296151 42.432 370 202 4 1 364 3 367 2.52e-93 284
MsaG045761 MtrunA17_Chr2g0296111 44.767 344 182 4 25 364 28 367 4.42e-93 283
MsaG045761 MtrunA17_Chr2g0295741 43.860 342 189 2 25 364 33 373 4.88e-92 281
MsaG045761 MtrunA17_Chr2g0296231 42.659 361 196 5 14 365 20 378 3.26e-91 279
MsaG045761 MtrunA17_Chr2g0296191 43.681 364 202 2 1 364 147 507 8.94e-91 294
MsaG045761 MtrunA17_Chr2g0296191 46.439 351 177 4 25 364 644 994 5.68e-90 291
MsaG045761 MtrunA17_Chr2g0296291 42.033 364 173 2 39 364 32 395 2.25e-89 275
MsaG045761 MtrunA17_Chr2g0286191 42.618 359 196 5 8 364 6 356 9.93e-87 266
MsaG045761 MtrunA17_Chr2g0296181 41.834 349 163 6 25 364 37 354 3.38e-77 243
MsaG045761 MtrunA17_Chr4g0001831 32.754 345 222 5 28 364 25 367 3.48e-61 201
MsaG045761 MtrunA17_Chr2g0296131 54.305 151 69 0 214 364 9 159 4.32e-53 174
MsaG045761 MtrunA17_Chr2g0296551 45.856 181 98 0 184 364 1 181 1.02e-50 168
MsaG045761 MtrunA17_Chr2g0296211 49.405 168 85 0 197 364 188 355 3.60e-49 170
MsaG045761 MtrunA17_Chr2g0296211 45.833 96 49 2 23 115 100 195 1.25e-15 77.4
MsaG045761 MtrunA17_Chr2g0296161 51.656 151 73 0 214 364 5 155 1.03e-48 162
MsaG045761 MtrunA17_Chr2g0296701 50.658 152 75 0 213 364 2 153 3.71e-48 161
MsaG045761 MtrunA17_Chr2g0286221 47.097 155 80 2 210 364 14 166 1.96e-39 138
MsaG045761 MtrunA17_Chr8g0377781 27.515 338 241 3 34 367 37 374 1.68e-36 136
MsaG045761 MtrunA17_Chr2g0296751 49.206 126 64 0 239 364 1 126 1.11e-35 128
MsaG045761 MtrunA17_Chr8g0377801 25.360 347 255 3 34 376 50 396 6.54e-35 132
MsaG045761 MtrunA17_Chr2g0295711 38.298 188 109 4 5 186 9 195 6.70e-35 127
MsaG045761 MtrunA17_Chr4g0067131 65.882 85 29 0 283 367 1 85 6.33e-34 120
MsaG045761 MtrunA17_Chr2g0295721 45.299 117 64 0 212 328 3 119 1.26e-29 110
MsaG045761 MtrunA17_Chr2g0295751 40.441 136 79 1 33 166 41 176 3.01e-26 109
MsaG045761 MtrunA17_Chr4g0067681 75.410 61 15 0 272 332 1 61 2.17e-25 97.8
MsaG045761 MtrunA17_Chr2g0296561 47.934 121 62 1 25 145 23 142 6.30e-25 99.4
MsaG045761 MtrunA17_Chr2g0294671 23.958 288 178 8 69 336 213 479 1.14e-13 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045761 AT5G07900.1 32.000 350 225 5 30 366 52 401 6.27e-64 209
MsaG045761 AT1G21150.1 28.534 382 252 6 1 362 4 384 2.23e-45 160
MsaG045761 AT1G21150.2 28.534 382 252 6 1 362 68 448 1.00e-44 160
MsaG045761 AT1G21150.3 28.529 333 230 4 37 362 17 348 1.05e-42 152
MsaG045761 AT1G61980.3 26.966 356 221 6 39 356 51 405 3.59e-41 150
MsaG045761 AT1G61980.2 26.966 356 221 6 39 356 51 405 3.59e-41 150
MsaG045761 AT1G61980.1 26.966 356 221 6 39 356 51 405 3.59e-41 150
MsaG045761 AT1G61990.2 28.329 413 238 10 1 364 5 408 7.05e-40 146
MsaG045761 AT1G61990.1 28.329 413 238 10 1 364 5 408 7.05e-40 146
MsaG045761 AT1G61970.2 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G61970.7 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G61970.6 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G61970.3 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G61970.5 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G61970.4 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G61970.1 26.230 366 229 8 39 364 51 415 4.98e-39 144
MsaG045761 AT1G62120.1 25.936 374 236 6 39 371 55 428 5.63e-39 144
MsaG045761 AT3G46950.1 26.214 412 226 9 27 364 37 444 3.16e-38 142
MsaG045761 AT1G62150.1 25.250 400 220 8 35 357 52 449 5.15e-36 137
MsaG045761 AT1G62110.1 26.173 405 220 9 39 364 51 455 2.67e-31 124
MsaG045761 AT1G62085.1 30.451 266 174 3 25 279 32 297 1.68e-29 119
MsaG045761 AT1G62085.1 30.252 119 80 2 255 370 343 461 3.02e-12 68.2
MsaG045761 AT1G62085.2 30.451 266 174 3 25 279 32 297 1.68e-29 119
MsaG045761 AT1G62085.2 30.252 119 80 2 255 370 343 461 3.02e-12 68.2
MsaG045761 AT5G64950.1 26.243 362 249 7 28 376 27 383 6.11e-27 110
MsaG045761 AT1G61960.1 28.736 261 178 5 26 279 34 293 7.22e-26 108
MsaG045761 AT1G61960.1 32.203 118 77 1 255 369 339 456 3.43e-15 77.0
MsaG045761 AT1G62010.1 23.894 339 217 6 73 372 71 407 1.05e-25 107
MsaG045761 AT5G23930.1 28.859 298 191 7 27 310 34 324 3.70e-25 106
MsaG045761 AT1G56380.1 23.097 381 242 12 32 364 7 384 3.07e-22 97.4
MsaG045761 AT1G56380.2 23.097 381 242 12 32 364 18 395 3.23e-22 97.4

Find 76 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTGAAACTTTGGCAAAA+TGG 0.240171 8:-76710445 None:intergenic
AACTCATCAGTCAAAATAAA+TGG 0.289834 8:-76711134 None:intergenic
TTTAGAGTAACGTAACATTT+TGG 0.318656 8:-76710174 None:intergenic
AAGGTCTGTGGAGGAGATAA+AGG 0.322778 8:+76710758 MsaT045761.1:CDS
CCAGATCCAATAATGATAAA+AGG 0.326336 8:-76710729 None:intergenic
ATGCATGTAAAATTGAATCT+TGG 0.336264 8:-76710896 None:intergenic
GGTGCTGCCATGAGCAATAA+AGG 0.386758 8:-76711005 None:intergenic
AGACTAAATCCGAAAAGAAT+TGG 0.387498 8:-76711026 None:intergenic
GACAGTGACAATAATGTCTT+GGG 0.388401 8:-76710490 None:intergenic
TTCCAAGGGCTTCAGTTATC+CGG 0.401783 8:+76711063 MsaT045761.1:CDS
TGCATGTAAAATTGAATCTT+GGG 0.402310 8:-76710895 None:intergenic
GTCTTGGGGAGAAGCACCTT+TGG 0.403398 8:-76710475 None:intergenic
ATCGAAAGGTTTGATAAAGA+AGG 0.405715 8:+76711094 MsaT045761.1:CDS
CGCTATTAAAGTTATATCTC+GGG 0.407277 8:+76711201 MsaT045761.1:CDS
AAGTTGGTGTCTTGAAGAAA+TGG 0.411701 8:+76710865 MsaT045761.1:CDS
GTGGAGGAGATAAAGGGGTT+AGG 0.414916 8:+76710765 MsaT045761.1:CDS
AGGTCTGTGGAGGAGATAAA+GGG 0.418294 8:+76710759 MsaT045761.1:CDS
GGCTGTCACTAAATCTCAAT+GGG 0.436061 8:+76710836 MsaT045761.1:CDS
CAAGTATTTACAATTTGCTT+AGG 0.436803 8:+76710700 MsaT045761.1:CDS
TTGGATCAAACAGCTTGGTT+GGG 0.439509 8:+76710980 MsaT045761.1:CDS
TTTGGATCAAACAGCTTGGT+TGG 0.442173 8:+76710979 MsaT045761.1:CDS
CAGATCGTAGCATAAACTTT+GGG 0.443853 8:-76710929 None:intergenic
TGGAGGAGATAAAGGGGTTA+GGG 0.443892 8:+76710766 MsaT045761.1:CDS
AGTTGGTGTCTTGAAGAAAT+GGG 0.446401 8:+76710866 MsaT045761.1:CDS
TTGGGATCCTTTATTGCTCA+TGG 0.455848 8:+76710998 MsaT045761.1:CDS
TGGCTGTCACTAAATCTCAA+TGG 0.459837 8:+76710835 MsaT045761.1:CDS
TTTATCTGAATCTGAAGAAA+TGG 0.460894 8:-76710217 None:intergenic
ACGCTATTAAAGTTATATCT+CGG 0.466280 8:+76711200 MsaT045761.1:CDS
TTTCTTCAAGACACCAACTT+TGG 0.474001 8:-76710862 None:intergenic
GCAGATCGTAGCATAAACTT+TGG 0.481781 8:-76710930 None:intergenic
GATGGGGTTTGAAACGATGA+TGG 0.491601 8:-76710418 None:intergenic
CTTCTTACCAAATCAAAAGC+AGG 0.493927 8:-76710561 None:intergenic
TGACAGTGACAATAATGTCT+TGG 0.497645 8:-76710491 None:intergenic
CGAGATCTAGCAATTGAAGT+TGG 0.500150 8:-76710630 None:intergenic
TCGAAAGGTTTGATAAAGAA+GGG 0.508372 8:+76711095 MsaT045761.1:CDS
GGGTGGAATCCTGCGAATGA+TGG 0.511891 8:-76710397 None:intergenic
CAGGGATTATTTGTTTGTCA+AGG 0.513847 8:-76710542 None:intergenic
TAAAAGAATCGGGTTTGTGA+GGG 0.515496 8:-76710329 None:intergenic
GTTTGTCAAGGCTTGAACAA+AGG 0.516951 8:-76710530 None:intergenic
GTAATAGCTCTTTGATCAGA+AGG 0.518685 8:-76710591 None:intergenic
ATGGGGTTTGAAACGATGAT+GGG 0.526804 8:-76710417 None:intergenic
AACCTTTCGATAAAAGATAC+CGG 0.544149 8:-76711082 None:intergenic
TCTCAATGGGATGCCAAAGT+TGG 0.546789 8:+76710849 MsaT045761.1:CDS
TACCGGATAACTGAAGCCCT+TGG 0.548805 8:-76711065 None:intergenic
ATGGTGGTGCTGAAAGAGAA+GGG 0.557322 8:-76710198 None:intergenic
CTGATGAGTTGTTTCTGCAA+CGG 0.559627 8:+76711147 MsaT045761.1:CDS
TTATTGGATCTGGTTTGAGA+AGG 0.563650 8:+76710739 MsaT045761.1:CDS
TCATACCTCATCAACAATTG+TGG 0.563809 8:+76710255 MsaT045761.1:CDS
ATAAAAGAATCGGGTTTGTG+AGG 0.565444 8:-76710330 None:intergenic
AATGGTGGTGCTGAAAGAGA+AGG 0.565965 8:-76710199 None:intergenic
GATGGATTGTATCTGATCGT+TGG 0.567441 8:-76710379 None:intergenic
AGATACAATCCATCATTCGC+AGG 0.572684 8:+76710388 MsaT045761.1:CDS
TAGAGAAAAGACTTCTTCCA+AGG 0.575654 8:+76711048 MsaT045761.1:CDS
TTCTTACCAAATCAAAAGCA+GGG 0.579986 8:-76710560 None:intergenic
GGGTTTGAAACGATGATGGG+TGG 0.584866 8:-76710414 None:intergenic
GGCTTGAACAAAGGAAACGA+GGG 0.588494 8:-76710521 None:intergenic
CTGATCGTTGGAGAAACCAT+GGG 0.591154 8:-76710367 None:intergenic
ATAAACCACAATTGTTGATG+AGG 0.592618 8:-76710260 None:intergenic
ATCTGGTTTGAGAAGGTCTG+TGG 0.594791 8:+76710746 MsaT045761.1:CDS
GGTCTGTGGAGGAGATAAAG+GGG 0.597103 8:+76710760 MsaT045761.1:CDS
TCTGATCGTTGGAGAAACCA+TGG 0.601444 8:-76710368 None:intergenic
GAAGCTTTGAGAGCATCTTG+TGG 0.608539 8:-76710285 None:intergenic
AAAGTTTATGCTACGATCTG+CGG 0.612569 8:+76710932 MsaT045761.1:CDS
GAGATATAACTTTAATAGCG+TGG 0.615989 8:-76711198 None:intergenic
AAAAGAATCGGGTTTGTGAG+GGG 0.622947 8:-76710328 None:intergenic
AGAGAAAAGACTTCTTCCAA+GGG 0.623231 8:+76711049 MsaT045761.1:CDS
GTTGGTGTCTTGAAGAAATG+GGG 0.624621 8:+76710867 MsaT045761.1:CDS
ATCTGAATCTGAAGAAATGG+TGG 0.644416 8:-76710214 None:intergenic
TGGTTTGAGAAGGTCTGTGG+AGG 0.646724 8:+76710749 MsaT045761.1:CDS
GCTATTAAAGTTATATCTCG+GGG 0.649807 8:+76711202 MsaT045761.1:CDS
AATAGAGAAGAGCTCTGAGG+CGG 0.651382 8:-76710140 None:intergenic
AGGCTTGAACAAAGGAAACG+AGG 0.672222 8:-76710522 None:intergenic
ATTAAAGTTATATCTCGGGG+AGG 0.712652 8:+76711205 MsaT045761.1:CDS
AGGAATAGAGAAGAGCTCTG+AGG 0.723754 8:-76710143 None:intergenic
GAGGCGGTGACAATGAAACA+TGG 0.747817 8:-76710124 None:intergenic
ACAGTGACAATAATGTCTTG+GGG 0.749194 8:-76710489 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 76710126 76711259 76710126 ID=MsaG045761
Chr8 mRNA 76710126 76711259 76710126 ID=MsaT045761.1;Parent=MsaG045761
Chr8 exon 76710126 76711259 76710126 ID=MsaT045761.1.exon1;Parent=MsaT045761.1
Chr8 CDS 76710126 76711259 76710126 ID=cds.MsaT045761.1;Parent=MsaT045761.1
Gene Sequence

>MsaT045761.1

ATGTTTCATTGTCACCGCCTCAGAGCTCTTCTCTATTCCTCCTCATCACCAAAATGTTACGTTACTCTAAAACCCTTCTCTTTCAGCACCACCATTTCTTCAGATTCAGATAAACAATCTTTCACAGTTTCATACCTCATCAACAATTGTGGTTTATCTCCACAAGATGCTCTCAAAGCTTCTAAACGTTTTCGTTTCAATACCCCTCACAAACCCGATTCTTTTATCTCCTTTTTCAAAACCCATGGTTTCTCCAACGATCAGATACAATCCATCATTCGCAGGATTCCACCCATCATCGTTTCAAACCCCATCAAAACCATTTTGCCAAAGTTTCAATTTTTAGCTTCCAAAGGTGCTTCTCCCCAAGACATTATTGTCACTGTCACTAAAAGCCCTCGTTTCCTTTGTTCAAGCCTTGACAAACAAATAATCCCTGCTTTTGATTTGGTAAGAAGTTTCTGTCCTTCTGATCAAAGAGCTATTACTATAATAAATTCTTATCCAACTTCAATTGCTAGATCTCGCATGAAACCAAATGTTCATTTTTTGCTTGATTTTGGAGTCAGTCGCGCAAGTATTTACAATTTGCTTAGGACTAGTCCTTTTATCATTATTGGATCTGGTTTGAGAAGGTCTGTGGAGGAGATAAAGGGGTTAGGGTTTGAACCTTCCAACTACAATTTTTGTGTAGCATTGGCGACAAAAATGGCTGTCACTAAATCTCAATGGGATGCCAAAGTTGGTGTCTTGAAGAAATGGGGCTTGTCCCAAGATTCAATTTTACATGCATATAAAAAGAACCCAAAGTTTATGCTACGATCTGCGGACAAACTCAATGCAGTGATGAGTTTTTGGATCAAACAGCTTGGTTGGGATCCTTTATTGCTCATGGCAGCACCAATTCTTTTCGGATTTAGTCTAGAGAAAAGACTTCTTCCAAGGGCTTCAGTTATCCGGTATCTTTTATCGAAAGGTTTGATAAAGAAGGGTGCTAGCTTAACTGCACCATTTATTTTGACTGATGAGTTGTTTCTGCAACGGTTTGTGAATCGTTTTGAAGAAGAAGTATCCACGCTATTAAAGTTATATCTCGGGGAGGATGCTAGCATCTGCTGCTTAGATAAGTTGTGA
Protein sequence

>MsaT045761.1

MFHCHRLRALLYSSSSPKCYVTLKPFSFSTTISSDSDKQSFTVSYLINNCGLSPQDALKASKRFRFNTPHKPDSFISFFKTHGFSNDQIQSIIRRIPPIIVSNPIKTILPKFQFLASKGASPQDIIVTVTKSPRFLCSSLDKQIIPAFDLVRSFCPSDQRAITIINSYPTSIARSRMKPNVHFLLDFGVSRASIYNLLRTSPFIIIGSGLRRSVEEIKGLGFEPSNYNFCVALATKMAVTKSQWDAKVGVLKKWGLSQDSILHAYKKNPKFMLRSADKLNAVMSFWIKQLGWDPLLLMAAPILFGFSLEKRLLPRASVIRYLLSKGLIKKGASLTAPFILTDELFLQRFVNRFEEEVSTLLKLYLGEDASICCLDKL*