AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045762

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045762 MtrunA17_Chr4g0067671 76.582 316 49 1 35 325 42 357 2.30e-172 483
MsaG045762 MtrunA17_Chr4g0067141 69.091 330 77 1 35 339 42 371 2.86e-156 443
MsaG045762 MtrunA17_Chr2g0295761 46.866 335 151 3 30 337 52 386 5.08e-96 290
MsaG045762 MtrunA17_Chr4g0067121 46.646 328 149 2 37 338 51 378 9.80e-95 287
MsaG045762 MtrunA17_Chr2g0296301 43.807 331 159 2 35 338 34 364 4.55e-92 280
MsaG045762 MtrunA17_Chr2g0296181 45.533 347 173 6 1 338 16 355 1.24e-87 268
MsaG045762 MtrunA17_Chr2g0296711 44.012 334 149 4 39 338 55 384 1.58e-85 264
MsaG045762 MtrunA17_Chr2g0295741 43.505 331 160 2 35 338 44 374 4.77e-85 262
MsaG045762 MtrunA17_Chr2g0296121 45.921 331 152 3 35 338 124 454 7.75e-85 264
MsaG045762 MtrunA17_Chr2g0296151 42.900 331 162 3 35 338 38 368 1.82e-83 258
MsaG045762 MtrunA17_Chr2g0296171 45.482 332 149 4 39 338 62 393 2.20e-83 258
MsaG045762 MtrunA17_Chr2g0295731 42.296 331 164 3 35 338 45 375 3.38e-83 257
MsaG045762 MtrunA17_Chr2g0315731 44.478 335 148 5 37 337 70 400 4.46e-83 258
MsaG045762 MtrunA17_Chr2g0296721 44.242 330 155 4 37 337 50 379 1.22e-82 256
MsaG045762 MtrunA17_Chr2g0296741 43.072 332 159 3 37 338 70 401 7.19e-82 255
MsaG045762 MtrunA17_Chr2g0296251 40.327 367 156 3 35 338 30 396 3.62e-81 252
MsaG045762 MtrunA17_Chr2g0296221 43.675 332 155 4 39 338 81 412 6.39e-80 250
MsaG045762 MtrunA17_Chr2g0296111 42.643 333 161 4 35 338 37 368 1.63e-79 248
MsaG045762 MtrunA17_Chr2g0296291 41.176 374 145 5 37 341 32 399 5.68e-79 247
MsaG045762 MtrunA17_Chr2g0296191 43.385 325 159 1 39 338 184 508 2.81e-77 256
MsaG045762 MtrunA17_Chr2g0296191 43.452 336 155 5 39 342 664 996 9.80e-75 249
MsaG045762 MtrunA17_Chr2g0296231 41.945 329 159 4 39 337 51 377 1.63e-75 238
MsaG045762 MtrunA17_Chr2g0286191 39.155 355 173 6 16 338 14 357 7.40e-73 230
MsaG045762 MtrunA17_Chr4g0001831 31.231 333 193 6 39 338 39 368 1.55e-47 164
MsaG045762 MtrunA17_Chr2g0296551 43.407 182 78 1 182 338 1 182 1.06e-43 149
MsaG045762 MtrunA17_Chr2g0296131 42.500 160 67 1 204 338 1 160 6.40e-37 130
MsaG045762 MtrunA17_Chr2g0296161 45.455 154 59 1 210 338 3 156 8.78e-36 127
MsaG045762 MtrunA17_Chr2g0296211 41.420 169 74 1 195 338 188 356 4.88e-35 131
MsaG045762 MtrunA17_Chr2g0296211 49.351 77 37 1 39 113 119 195 4.23e-14 72.8
MsaG045762 MtrunA17_Chr2g0295711 43.151 146 76 2 35 173 44 189 8.53e-34 124
MsaG045762 MtrunA17_Chr2g0296701 40.523 153 66 1 211 338 2 154 1.28e-30 114
MsaG045762 MtrunA17_Chr8g0377781 25.959 339 222 4 30 339 35 373 3.15e-28 113
MsaG045762 MtrunA17_Chr2g0296561 53.333 105 49 0 39 143 38 142 9.06e-27 103
MsaG045762 MtrunA17_Chr2g0295751 43.750 128 69 2 39 164 49 175 1.11e-25 107
MsaG045762 MtrunA17_Chr2g0286221 38.037 163 74 3 201 338 7 167 3.15e-24 97.8
MsaG045762 MtrunA17_Chr8g0377801 23.952 334 225 5 35 339 53 386 8.62e-24 100
MsaG045762 MtrunA17_Chr2g0296751 39.370 127 52 1 237 338 1 127 1.99e-22 92.4
MsaG045762 MtrunA17_Chr4g0067131 61.644 73 28 0 267 339 12 84 1.16e-21 87.8
MsaG045762 MtrunA17_Chr2g0295721 40.351 114 43 1 210 298 3 116 1.25e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045762 AT5G07900.1 32.370 346 196 6 28 339 60 401 3.28e-55 186
MsaG045762 AT1G21150.1 29.394 330 202 5 37 335 55 384 4.30e-38 140
MsaG045762 AT1G21150.3 28.955 335 207 5 32 335 14 348 1.32e-37 138
MsaG045762 AT1G21150.2 29.394 330 202 5 37 335 119 448 1.45e-37 140
MsaG045762 AT1G62120.1 25.341 367 208 6 39 339 57 423 4.53e-34 130
MsaG045762 AT3G46950.1 31.760 233 154 4 39 270 53 281 1.11e-32 126
MsaG045762 AT1G61980.3 26.087 368 206 8 37 339 51 417 3.34e-29 116
MsaG045762 AT1G61980.2 26.087 368 206 8 37 339 51 417 3.34e-29 116
MsaG045762 AT1G61980.1 26.087 368 206 8 37 339 51 417 3.34e-29 116
MsaG045762 AT1G61970.2 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G61970.7 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G61970.6 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G61970.3 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G61970.5 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G61970.4 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G61970.1 27.985 268 177 4 2 267 29 282 3.41e-29 116
MsaG045762 AT1G62085.1 30.638 235 160 2 39 270 56 290 3.50e-29 117
MsaG045762 AT1G62085.2 30.638 235 160 2 39 270 56 290 3.50e-29 117
MsaG045762 AT1G62110.1 27.935 247 175 2 39 282 53 299 2.74e-28 114
MsaG045762 AT1G61990.2 29.614 233 156 4 39 270 53 278 4.65e-27 110
MsaG045762 AT1G61990.1 29.614 233 156 4 39 270 53 278 4.65e-27 110
MsaG045762 AT1G61960.1 28.226 248 169 5 30 270 41 286 9.43e-26 107
MsaG045762 AT5G23930.1 26.978 278 186 6 36 312 53 314 1.10e-25 107
MsaG045762 AT5G64950.1 27.164 335 207 6 41 338 42 376 2.29e-23 100
MsaG045762 AT1G62150.1 25.498 251 176 4 39 286 59 301 2.79e-23 100
MsaG045762 AT1G62010.1 25.214 234 162 4 54 282 56 281 1.83e-19 89.0
MsaG045762 AT1G56380.1 22.314 363 201 10 41 329 19 374 1.77e-17 83.2
MsaG045762 AT1G56380.2 22.314 363 201 10 41 329 30 385 2.63e-17 82.8

Find 97 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGGAGATTAAGGAATT+AGG 0.141632 8:-76715724 MsaT045762.1:CDS
TTGTTCTGAAGATGATATTT+TGG 0.214482 8:-76715599 MsaT045762.1:CDS
AAAGGCTTTGGAGGAGATTA+AGG 0.223358 8:-76715731 MsaT045762.1:CDS
ATGCTCTACTGATTTGAAAA+AGG 0.232177 8:-76715749 MsaT045762.1:CDS
AAAGATGATTCCTCTGTTAT+AGG 0.255958 8:-76717326 MsaT045762.1:intron
GGACTTGGTGACAGCCCTTT+TGG 0.271549 8:+76715660 None:intergenic
AACTCGTCCTTCAAATAAAA+TGG 0.276985 8:+76715358 None:intergenic
CAAAAGTATGTGAAACGTTT+TGG 0.285164 8:-76715328 MsaT045762.1:CDS
TCCAGGCACACCCGTTCTTT+TGG 0.302599 8:-76715475 MsaT045762.1:intron
CATGCTATTAAAGCTATATC+AGG 0.320327 8:-76715293 MsaT045762.1:CDS
CTTTGGCAAAATTGATTTGA+TGG 0.324194 8:+76716053 None:intergenic
TGGAGGAGATTAAGGAATTA+GGG 0.324678 8:-76715723 MsaT045762.1:CDS
TCATCTTTCACTCTATCATT+TGG 0.349582 8:+76717341 None:intergenic
GTCTTGGGGAGAAGCACCTT+TGG 0.383783 8:+76716014 None:intergenic
AACGGTGGCAACAATGTCTT+GGG 0.392461 8:+76715999 None:intergenic
GAGGCTTGAAGCAAGGAAAC+GGG 0.394445 8:+76715966 None:intergenic
GTATCTTCTTTCCAAAGGTT+TGG 0.398152 8:-76715407 MsaT045762.1:CDS
TCCGATGCCGACGCCCTCGC+TGG 0.408614 8:-76717411 None:intergenic
CGAGACCGTAGCATTAAGTT+AGG 0.409449 8:+76715559 None:intergenic
AGTTGATGCCTTGAAGAGTT+GGG 0.411739 8:-76715623 MsaT045762.1:CDS
GATAACGGAGTCGGGTTTGT+TGG 0.416971 8:+76716158 None:intergenic
CTTCTTACCAATTCAAAAGA+AGG 0.421065 8:+76715928 None:intergenic
TGCGAATGATGGTTTGTATC+TGG 0.432299 8:+76716103 None:intergenic
TTGTTGGTGAGGTAGGAAAC+TGG 0.434401 8:+76716240 None:intergenic
GTGTGGCATTATTGGCCAAA+AGG 0.435093 8:-76715675 MsaT045762.1:CDS
TTTGGCATCCCATTGGGACT+TGG 0.436785 8:+76715645 None:intergenic
ACTCTTCAAGGCATCAACTT+TGG 0.437967 8:+76715627 None:intergenic
ATCCTCCTTCACCAAACCTT+TGG 0.439740 8:+76715396 None:intergenic
ATAACGGAGTCGGGTTTGTT+GGG 0.446265 8:+76716159 None:intergenic
AACCTTTGGAAAGAAGATAC+TGG 0.447858 8:+76715410 None:intergenic
ATCAACTTTGGCATCCCATT+GGG 0.451567 8:+76715639 None:intergenic
AGAACAACCCCAACTCTTCA+AGG 0.455014 8:+76715615 None:intergenic
TATACTAAATCCAAAAGAAC+GGG 0.460939 8:+76715465 None:intergenic
TTTGGCAAAATTGATTTGAT+GGG 0.461357 8:+76716054 None:intergenic
CGAGGCTTGAAGCAAGGAAA+CGG 0.463064 8:+76715965 None:intergenic
AAGCGAGAATCACGAATTGT+AGG 0.464569 8:+76715856 None:intergenic
GTCCAGTATCTTCTTTCCAA+AGG 0.465341 8:-76715412 MsaT045762.1:CDS
GGCTGTCACCAAGTCCCAAT+GGG 0.471957 8:-76715653 MsaT045762.1:CDS
CATCAACTTTGGCATCCCAT+TGG 0.475903 8:+76715638 None:intergenic
TCTTTCACTCTATCATTTGG+CGG 0.477631 8:+76717344 None:intergenic
TAACGGTGGCAACAATGTCT+TGG 0.479132 8:+76715998 None:intergenic
AGCGAGAATCACGAATTGTA+GGG 0.480852 8:+76715857 None:intergenic
AGTGTTGAAACGGAGTCGTT+TGG 0.481325 8:+76716182 None:intergenic
TTGGATTTAGTATAGAGAAA+AGG 0.482494 8:-76715456 MsaT045762.1:CDS
TTGGGGAGAAGCACCTTTGG+AGG 0.490963 8:+76716017 None:intergenic
TCCAGCGAGGGCGTCGGCAT+CGG 0.494603 8:+76717410 None:intergenic
AAGTTGATGCCTTGAAGAGT+TGG 0.496376 8:-76715624 MsaT045762.1:CDS
TTTGGCGGCGGAAGCAATTG+AGG 0.496400 8:+76717359 None:intergenic
AGGGTCTAGTAGTAAGCAAA+TGG 0.503507 8:+76715779 None:intergenic
GTTTGTTGGGAGTGTTGAAA+CGG 0.507083 8:+76716172 None:intergenic
AGACATGAACCCACGTATGA+TGG 0.516547 8:+76717386 None:intergenic
CTGATATAGCTTTAATAGCA+TGG 0.517550 8:+76715294 None:intergenic
TGGAGATAAACCGCAGTTGT+TGG 0.517833 8:+76716224 None:intergenic
TTCTTACCAATTCAAAAGAA+GGG 0.521054 8:+76715929 None:intergenic
CTCGCTGGAACCATCATACG+TGG 0.522134 8:-76717396 MsaT045762.1:CDS
TGTGGCATTATTGGCCAAAA+GGG 0.530442 8:-76715674 MsaT045762.1:CDS
CGATGGAATCCTGCGAATGA+TGG 0.539386 8:+76716092 None:intergenic
CTATACTAAATCCAAAAGAA+CGG 0.541387 8:+76715464 None:intergenic
TTCCAAAGGTTTGGTGAAGG+AGG 0.548687 8:-76715398 MsaT045762.1:CDS
GGGCTGTCACCAAGTCCCAA+TGG 0.553920 8:-76715654 MsaT045762.1:CDS
GACAACCTAACTTAATGCTA+CGG 0.560893 8:-76715564 MsaT045762.1:intron
GTAATAGCTTTATGATCAGA+AGG 0.568712 8:+76715898 None:intergenic
ACCGCAGTTGTTGGTGAGGT+AGG 0.574996 8:+76716233 None:intergenic
GGGTTTGAAACGATGAGCGA+TGG 0.579479 8:+76716075 None:intergenic
TCTTTCCAAAGGTTTGGTGA+AGG 0.579695 8:-76715401 MsaT045762.1:CDS
ACGTATGATGGTTCCAGCGA+GGG 0.580392 8:+76717398 None:intergenic
TTGGCAAAATTGATTTGATG+GGG 0.581662 8:+76716055 None:intergenic
AGGCTTGAAGCAAGGAAACG+GGG 0.582746 8:+76715967 None:intergenic
AGATACAAACCATCATTCGC+AGG 0.586629 8:-76716101 MsaT045762.1:CDS
TTCACTCTATCATTTGGCGG+CGG 0.591064 8:+76717347 None:intergenic
ATCAGTAGAGCATATTGTAG+AGG 0.594418 8:+76715759 None:intergenic
GATGGTTCCAGCGAGGGCGT+CGG 0.595266 8:+76717404 None:intergenic
TTAAAGCTATATCAGGGAGG+AGG 0.595436 8:-76715286 MsaT045762.1:CDS
AGAGAAAAGGCTTATTCCGA+GGG 0.601279 8:-76715443 MsaT045762.1:CDS
ATGCTATTAAAGCTATATCA+GGG 0.602620 8:-76715292 MsaT045762.1:CDS
TGTTGGTGAGGTAGGAAACT+GGG 0.604893 8:+76716241 None:intergenic
GATGCTTTGAGAGCGTCTTG+TGG 0.606368 8:+76716204 None:intergenic
AAGGGATTATGTACTTCTCG+AGG 0.610017 8:+76715947 None:intergenic
TCAGTAGAGCATATTGTAGA+GGG 0.610595 8:+76715760 None:intergenic
TCTGGTGATCGGAGAAACCG+TGG 0.611970 8:+76716121 None:intergenic
TACTGGACAACAGAAGCCCT+CGG 0.620230 8:+76715427 None:intergenic
GATGGTTTGTATCTGGTGAT+CGG 0.623257 8:+76716110 None:intergenic
GTTGATGCCTTGAAGAGTTG+GGG 0.626644 8:-76715622 MsaT045762.1:CDS
TAGAGAAAAGGCTTATTCCG+AGG 0.626765 8:-76715444 MsaT045762.1:CDS
ACTTCTCGAGGCTTGAAGCA+AGG 0.627237 8:+76715959 None:intergenic
CGAGAATCACGAATTGTAGG+GGG 0.639288 8:+76715859 None:intergenic
TCCTACCTCACCAACAACTG+CGG 0.644241 8:-76716234 MsaT045762.1:CDS
ATAAACCGCAGTTGTTGGTG+AGG 0.646663 8:+76716229 None:intergenic
GGCTTGAAGCAAGGAAACGG+GGG 0.647192 8:+76715968 None:intergenic
CATTCTATGACCTATAACAG+AGG 0.651607 8:+76717316 None:intergenic
GCGAGAATCACGAATTGTAG+GGG 0.656253 8:+76715858 None:intergenic
CACGTATGATGGTTCCAGCG+AGG 0.680199 8:+76717397 None:intergenic
TCGCTGGAACCATCATACGT+GGG 0.689121 8:-76717395 MsaT045762.1:CDS
CTGGTGATCGGAGAAACCGT+GGG 0.719461 8:+76716122 None:intergenic
GAGAATCACGAATTGTAGGG+GGG 0.721144 8:+76715860 None:intergenic
CTATTAAAGCTATATCAGGG+AGG 0.766585 8:-76715289 MsaT045762.1:CDS
ACGGTGGCAACAATGTCTTG+GGG 0.780918 8:+76716000 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 76715279 76717429 76715279 ID=MsaG045762
Chr8 mRNA 76715279 76717429 76715279 ID=MsaT045762.1;Parent=MsaG045762
Chr8 exon 76715279 76715492 76715279 ID=MsaT045762.1.exon3;Parent=MsaT045762.1
Chr8 CDS 76715279 76715492 76715279 ID=cds.MsaT045762.1;Parent=MsaT045762.1
Chr8 exon 76715565 76716276 76715565 ID=MsaT045762.1.exon2;Parent=MsaT045762.1
Chr8 CDS 76715565 76716276 76715565 ID=cds.MsaT045762.1;Parent=MsaT045762.1
Chr8 exon 76717327 76717429 76717327 ID=MsaT045762.1.exon1;Parent=MsaT045762.1
Chr8 CDS 76717327 76717429 76717327 ID=cds.MsaT045762.1;Parent=MsaT045762.1
Gene Sequence

>MsaT045762.1

ATGCCGACGCCCTCGCTGGAACCATCATACGTGGGTTCATGTCTTACTCCTCAATTGCTTCCGCCGCCAAATGATAGAGTGAAAGATGATTCCTCTGTTATAGATCAACAATGTTTCCCAGTTTCCTACCTCACCAACAACTGCGGTTTATCTCCACAAGACGCTCTCAAAGCATCCAAACGACTCCGTTTCAACACTCCCAACAAACCCGACTCCGTTATCGCCTTTTTCAAAACCCACGGTTTCTCCGATCACCAGATACAAACCATCATTCGCAGGATTCCATCGCTCATCGTTTCAAACCCCATCAAATCAATTTTGCCAAAGTTTCAATTTTTAGCCTCCAAAGGTGCTTCTCCCCAAGACATTGTTGCCACCGTTACTAGAAGCCCCCGTTTCCTTGCTTCAAGCCTCGAGAAGTACATAATCCCTTCTTTTGAATTGGTAAGAAGTTTTTGTCCTTCTGATCATAAAGCTATTACTTCAATTATCGTTTGCCCCCCTACAATTCGTGATTCTCGCTTGAAACCAAATCTTCAATTTTTGCTTGATTTTGGTGTCACTCGTTCAAGTATTTACCATTTGCTTACTACTAGACCCTCTACAATATGCTCTACTGATTTGAAAAAGGCTTTGGAGGAGATTAAGGAATTAGGGTTTCAACCTTCCAAATACAATTTTTGTGTGGCATTATTGGCCAAAAGGGCTGTCACCAAGTCCCAATGGGATGCCAAAGTTGATGCCTTGAAGAGTTGGGGTTGTTCTGAAGATGATATTTTGGGTGCATTTAAAAGACAACCTAACTTAATGCTACGGCACACCCGTTCTTTTGGATTTAGTATAGAGAAAAGGCTTATTCCGAGGGCTTCTGTTGTCCAGTATCTTCTTTCCAAAGGTTTGGTGAAGGAGGATGCTAGCTTAACTGCACCATTTTATTTGAAGGACGAGTTGTTCCTGCAAAAGTATGTGAAACGTTTTGGAGACGAAGCTTCCATGCTATTAAAGCTATATCAGGGAGGAGGATGCTAG
Protein sequence

>MsaT045762.1

MPTPSLEPSYVGSCLTPQLLPPPNDRVKDDSSVIDQQCFPVSYLTNNCGLSPQDALKASKRLRFNTPNKPDSVIAFFKTHGFSDHQIQTIIRRIPSLIVSNPIKSILPKFQFLASKGASPQDIVATVTRSPRFLASSLEKYIIPSFELVRSFCPSDHKAITSIIVCPPTIRDSRLKPNLQFLLDFGVTRSSIYHLLTTRPSTICSTDLKKALEEIKELGFQPSKYNFCVALLAKRAVTKSQWDAKVDALKSWGCSEDDILGAFKRQPNLMLRHTRSFGFSIEKRLIPRASVVQYLLSKGLVKEDASLTAPFYLKDELFLQKYVKRFGDEASMLLKLYQGGGC*