AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045781

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045781 MtrunA17_Chr4g0067401 98.725 863 11 0 2 864 1 863 0.0 1779
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045781 AT4G15840.1 59.356 839 287 16 35 864 43 836 0.0 929
MsaG045781 AT4G15840.2 56.475 749 272 16 35 774 43 746 0.0 768

Find 254 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCCTTGGCATATCTTTA+TGG 0.134615 8:+76923004 MsaT045781.1:CDS
CATTATTATCATTAAGTTTA+GGG 0.245800 8:-76923033 None:intergenic
GCATTATTATCATTAAGTTT+AGG 0.249784 8:-76923034 None:intergenic
GCACTACAGCAATAACTCTT+AGG 0.253937 8:-76927490 None:intergenic
TCTCCCGCAGTTCATATTTC+AGG 0.259529 8:+76922311 MsaT045781.1:CDS
CGCTTCCTTTCTCGACCTTC+AGG 0.263772 8:+76923075 MsaT045781.1:CDS
TGACCTGTCTTCTGCCTATA+TGG 0.267976 8:-76924392 None:intergenic
CAAATTGAAGGTTTCAATTC+CGG 0.268850 8:+76922226 MsaT045781.1:CDS
CATTGTTATCATCGATTTGC+AGG 0.269175 8:-76922961 None:intergenic
GAATCTTCAGGTCAGTATTC+AGG 0.272395 8:+76926727 MsaT045781.1:intron
TTCAAACTTCTTGGCTTATC+AGG 0.272512 8:+76923287 MsaT045781.1:CDS
AGCAGCACTGGCTTCTTTCC+AGG 0.278200 8:-76922930 None:intergenic
CCCCATCCTTTAGGAGCTTT+AGG 0.283620 8:-76927413 None:intergenic
CTTAGCCAGGGAGGGAATTA+TGG 0.289922 8:+76925430 MsaT045781.1:CDS
TACCAAGGGTGCGCCGTCCC+TGG 0.292685 8:-76926769 None:intergenic
TTGCCTTGGCATATCTTTAT+GGG 0.305502 8:+76923005 MsaT045781.1:CDS
GGTGAAGGAGGAGGGTTGTT+TGG 0.306761 8:-76922079 None:intergenic
CAACAGTTCTTCTATATCAT+TGG 0.307501 8:+76925594 MsaT045781.1:CDS
GCTATGGACTTCAAACTTCT+TGG 0.308454 8:+76923278 MsaT045781.1:CDS
ATGGAACCCCTTTGCTTTCA+TGG 0.311933 8:+76924364 MsaT045781.1:CDS
CTTCATGAAAAGGTTTGAAT+TGG 0.312775 8:+76923777 MsaT045781.1:intron
GAACCAGCATAGAAAACTTC+TGG 0.314399 8:-76926614 None:intergenic
GTTCGCCAACCCCATCCTTT+AGG 0.316184 8:-76927422 None:intergenic
TGCGAACATGTTCAAATTGA+AGG 0.319930 8:+76922214 MsaT045781.1:CDS
CTTGCTGATCTTCTACAAAA+TGG 0.320260 8:+76927464 MsaT045781.1:CDS
AATCTCCAAGCTCAACTAAA+AGG 0.323259 8:-76924005 None:intergenic
GCGTGTTAGAACTGCTTGTT+GGG 0.332796 8:+76923447 MsaT045781.1:CDS
ACCAAGGGTGCGCCGTCCCT+GGG 0.347154 8:-76926768 None:intergenic
GGTGCTATTGATGGGCTTGC+AGG 0.348289 8:+76925223 MsaT045781.1:CDS
TTCATCTCTATAGTTTCCTC+TGG 0.349358 8:-76924424 None:intergenic
TTTGCGGAGAACCAAGATTA+TGG 0.353090 8:+76923412 MsaT045781.1:CDS
GGAATTCCTGGTTCTGAGTC+TGG 0.355047 8:+76923856 MsaT045781.1:CDS
CATCCTGCACATGGAATTCC+TGG 0.358555 8:+76923844 MsaT045781.1:CDS
AACACAAAGGGAACAAGAAA+TGG 0.359332 8:+76925474 MsaT045781.1:CDS
ATTGGCTTTCCACACATTCC+TGG 0.360070 8:+76923795 MsaT045781.1:CDS
GGAAGCGCTGCGTTGGTGAT+AGG 0.361656 8:-76922101 None:intergenic
AAAGGTGACTGCACGGTATC+AGG 0.365867 8:+76927228 MsaT045781.1:CDS
GTTGAGGTGCCTCTTGGTGC+TGG 0.374916 8:+76924192 MsaT045781.1:CDS
CATTGCATTAACAACTATAT+CGG 0.379133 8:-76922257 None:intergenic
TCCATCGTATCCTTGTGATA+TGG 0.379836 8:+76924110 MsaT045781.1:CDS
ATTGATGGGCTTGCAGGTAT+TGG 0.382051 8:+76925229 MsaT045781.1:CDS
TCCAGTGAATGGGGAAGATA+TGG 0.382120 8:+76924345 MsaT045781.1:CDS
TGGATTCAAGTTTGGTGAAA+TGG 0.384240 8:-76924091 None:intergenic
TGATATGGAAGGACCTTCAT+TGG 0.385172 8:+76924125 MsaT045781.1:CDS
TGATTCAGGAACATGCTTCA+TGG 0.385881 8:+76922905 MsaT045781.1:intron
AACCAAGATTATGGCATTCA+TGG 0.388083 8:+76923421 MsaT045781.1:CDS
TGGAACCCCTTTGCTTTCAT+GGG 0.402678 8:+76924365 MsaT045781.1:CDS
CTTTCATGGGGTGGCCATAT+AGG 0.405787 8:+76924378 MsaT045781.1:CDS
GGATACGATGGATTCAAGTT+TGG 0.407077 8:-76924099 None:intergenic
CACTTACCGCTTCTCTTCAC+AGG 0.408715 8:-76923682 None:intergenic
AGCAAGTTGAACCCGTTGAT+TGG 0.412078 8:-76924061 None:intergenic
AGCTTTAGTCGGGCTTAGCC+AGG 0.412510 8:+76925417 MsaT045781.1:CDS
CTTGGTGCTGGAACAACTAC+TGG 0.416579 8:+76924204 MsaT045781.1:CDS
ACCCCTTTGCTTTCATGGGG+TGG 0.419158 8:+76924369 MsaT045781.1:CDS
CCCCAGGGACGGCGCACCCT+TGG 0.419963 8:+76926767 MsaT045781.1:CDS
CATGTTAGAGGTGCTATTGA+TGG 0.422366 8:+76925214 MsaT045781.1:CDS
CATGATGTTCTCACCCCGTC+TGG 0.423356 8:-76924165 None:intergenic
AGAAAGCAGCTTGAAGAGAT+AGG 0.429338 8:+76924525 MsaT045781.1:CDS
TGGTGATAGGGTGAAGGAGG+AGG 0.433042 8:-76922088 None:intergenic
GAACACCAACATTTCCCTCT+TGG 0.435857 8:-76927360 None:intergenic
AAGCTGTAAATGATGGTCTA+TGG 0.436191 8:+76924559 MsaT045781.1:CDS
AACATACCATTGGTATAGAA+TGG 0.436452 8:+76925563 MsaT045781.1:CDS
CATTTCTGATTCCAATCAAC+GGG 0.437158 8:+76924050 MsaT045781.1:CDS
TGGGCTCACTTCCAGTGAAT+GGG 0.438222 8:+76924335 MsaT045781.1:CDS
GTGGGCTCACTTCCAGTGAA+TGG 0.440603 8:+76924334 MsaT045781.1:CDS
GGTGGTTGGATCATTGTTAT+CGG 0.441545 8:-76922152 None:intergenic
GATATAGTTGTTAATGCAAT+GGG 0.442170 8:+76922259 MsaT045781.1:CDS
GACTGTTTACTAGGTGTTTG+CGG 0.444119 8:+76923396 MsaT045781.1:intron
ACCTATTGTTGTTATCTGAA+TGG 0.444189 8:-76924266 None:intergenic
TTCGGAAGCTTTAAACAAAC+GGG 0.444816 8:+76927380 MsaT045781.1:CDS
CAAGATGTTGGAAACTGACT+TGG 0.448497 8:+76923936 MsaT045781.1:CDS
TTTCTGTTGCCAACGCCAAA+TGG 0.451275 8:-76925316 None:intergenic
CGATATAGTTGTTAATGCAA+TGG 0.451581 8:+76922258 MsaT045781.1:CDS
CCTGGTTCTGAGTCTGGAAC+TGG 0.451697 8:+76923862 MsaT045781.1:CDS
TCTTCTACAGGTGAAAGAAA+TGG 0.451971 8:+76925169 MsaT045781.1:CDS
CCCCATGAATGCCATAATCT+TGG 0.453093 8:-76923423 None:intergenic
AGCGTGTTAGAACTGCTTGT+TGG 0.455113 8:+76923446 MsaT045781.1:CDS
CTATGCTGGTTCTTTGTGGA+AGG 0.455390 8:+76926625 MsaT045781.1:CDS
AATGTATGAGATGAAAGTTT+AGG 0.458406 8:-76923624 None:intergenic
CCTTGCAAAGCTGTAAATGA+TGG 0.459493 8:+76924552 MsaT045781.1:CDS
GTTCGGAAGCTTTAAACAAA+CGG 0.461060 8:+76927379 MsaT045781.1:CDS
TGGTGAGATGATGGCTGCTT+GGG 0.462275 8:-76922127 None:intergenic
GTATTTCTTAATATATTTGA+AGG 0.462512 8:+76924453 MsaT045781.1:CDS
ATGGGATTGCAAAGTACTGC+TGG 0.466131 8:+76925493 MsaT045781.1:CDS
ACCATTCAGATAACAACAAT+AGG 0.466623 8:+76924265 MsaT045781.1:CDS
ATGGATTGACAGCTTTAGTC+GGG 0.469334 8:+76925407 MsaT045781.1:CDS
TGACTTACCCAAAGCGAAAA+GGG 0.470869 8:-76925645 None:intergenic
TCCATATCTTCCCCATTCAC+TGG 0.470957 8:-76924346 None:intergenic
GATCACAATGGAGACCTGTC+TGG 0.471883 8:+76925382 MsaT045781.1:CDS
TCTATACCAATGGTATGTTC+TGG 0.473773 8:-76925559 None:intergenic
GAGGGTTGTTTGGATATTGA+GGG 0.475120 8:-76922069 None:intergenic
GGAGGGTTGTTTGGATATTG+AGG 0.475128 8:-76922070 None:intergenic
CATAATCATAACAATATACC+TGG 0.475480 8:+76925118 MsaT045781.1:CDS
TTTCATCGAGTTCTTGTAGT+GGG 0.476647 8:+76924316 MsaT045781.1:CDS
ACCAACATTTCCCTCTTGGA+TGG 0.478200 8:-76927356 None:intergenic
ACTACTGGAGTGGGTATTGA+AGG 0.478672 8:+76924219 MsaT045781.1:CDS
TCTCGTGTGCCATTTGGCGT+TGG 0.481287 8:+76925307 MsaT045781.1:CDS
GGCCACCCCATGAAAGCAAA+GGG 0.481323 8:-76924371 None:intergenic
GCACAGGCTTGGTGATGGCC+AGG 0.485817 8:+76926580 MsaT045781.1:CDS
CAGGGTCACAATAGCAGCAC+TGG 0.488664 8:-76922942 None:intergenic
TCTGATAACACCAAGGGAAA+AGG 0.488742 8:+76923892 MsaT045781.1:CDS
GAGTCTTAAAAGTGATCTAA+AGG 0.489618 8:+76923966 MsaT045781.1:CDS
GGGTATCCACAGATCATTAG+AGG 0.490981 8:-76923661 None:intergenic
CACTACAGCAATAACTCTTA+GGG 0.492494 8:-76927489 None:intergenic
CGAGGCTATTGCAATTGCCT+TGG 0.492878 8:+76922991 MsaT045781.1:CDS
TGAAATATGAACTGCGGGAG+AGG 0.493342 8:-76922309 None:intergenic
CTGACTTACCCAAAGCGAAA+AGG 0.493778 8:-76925646 None:intergenic
ACACAAAGGGAACAAGAAAT+GGG 0.495586 8:+76925475 MsaT045781.1:CDS
AAGAGGGAAATGTTGGTGTT+CGG 0.499834 8:+76927362 MsaT045781.1:CDS
GACAATGAATTACCCAATGA+AGG 0.502773 8:-76924138 None:intergenic
GAACCAGGAATTCCATGTGC+AGG 0.505773 8:-76923847 None:intergenic
GTTGTTATCTGAATGGTAGC+AGG 0.506164 8:-76924259 None:intergenic
TGTTATGTTGAGGTGCCTCT+TGG 0.506254 8:+76924186 MsaT045781.1:CDS
CTTCTACCTAAAGCTCCTAA+AGG 0.507698 8:+76927407 MsaT045781.1:CDS
TTTGAGGAAGTGCACAGGCT+TGG 0.507777 8:+76926569 MsaT045781.1:CDS
GGAACATGCTTCATGGTCCC+TGG 0.508066 8:+76922912 MsaT045781.1:intron
ATGTTCTTGTATGTAAGATC+CGG 0.508845 8:-76925098 None:intergenic
TTGTTCCATAATTCCCTCCC+TGG 0.509226 8:-76925435 None:intergenic
ACCTAAAGCTCCTAAAGGAT+GGG 0.509271 8:+76927412 MsaT045781.1:CDS
TGGTGTCTCTAACAATTGTA+CGG 0.509645 8:-76925539 None:intergenic
GGTGATAGGGTGAAGGAGGA+GGG 0.510194 8:-76922087 None:intergenic
TATGTTGCTGAATCTTCTAC+AGG 0.511758 8:+76925157 MsaT045781.1:CDS
CCAGTTCCAGACTCAGAACC+AGG 0.512258 8:-76923862 None:intergenic
TATTTCTTAATATATTTGAA+GGG 0.512575 8:+76924454 MsaT045781.1:CDS
AGATGATGGCTGCTTGGGAA+TGG 0.512683 8:-76922122 None:intergenic
CCTGTACGGGTACATGTTAG+AGG 0.513408 8:+76925202 MsaT045781.1:CDS
ACGTGCTTCTGAATCATCAT+TGG 0.515180 8:-76925356 None:intergenic
GTAGGAGGTGAAGCCGCTGC+AGG 0.516779 8:-76925271 None:intergenic
ACATACCATTGGTATAGAAT+GGG 0.517714 8:+76925564 MsaT045781.1:CDS
CTGTCAATCCATCTCCAGAC+AGG 0.521042 8:-76925396 None:intergenic
GATATGGAAGGACCTTCATT+GGG 0.523399 8:+76924126 MsaT045781.1:CDS
ACCAAGATTATGGCATTCAT+GGG 0.523479 8:+76923422 MsaT045781.1:CDS
TACCTAAAGCTCCTAAAGGA+TGG 0.524520 8:+76927411 MsaT045781.1:CDS
TCATCTCTATAGTTTCCTCT+GGG 0.527103 8:-76924423 None:intergenic
CCCATCCAAGAGGGAAATGT+TGG 0.527624 8:+76927355 MsaT045781.1:CDS
ATGTTAGAGGTGCTATTGAT+GGG 0.529897 8:+76925215 MsaT045781.1:CDS
AATGGAGACCTGTCTGGAGA+TGG 0.530070 8:+76925388 MsaT045781.1:CDS
TTCAGATGCTTCTTAGTCAG+AGG 0.530461 8:+76924978 MsaT045781.1:intron
GTTGAGCTTGGAGATTCAGA+AGG 0.532108 8:+76924012 MsaT045781.1:CDS
CCAAGCTCAACTAAAAGGTT+AGG 0.533794 8:-76924000 None:intergenic
ATTTCATTATATCTGAGCTA+TGG 0.534101 8:+76923262 MsaT045781.1:CDS
ATGGTGAGATGATGGCTGCT+TGG 0.535393 8:-76922128 None:intergenic
GATGGATTGACAGCTTTAGT+CGG 0.536660 8:+76925406 MsaT045781.1:CDS
TCAGATGCTTCTTAGTCAGA+GGG 0.537873 8:+76924979 MsaT045781.1:intron
GCACGTAATTCAGCGGTGGT+TGG 0.538329 8:-76922166 None:intergenic
CCAAGGGTGCGCCGTCCCTG+GGG 0.538403 8:-76926767 None:intergenic
GAAGCGCTGCGTTGGTGATA+GGG 0.547226 8:-76922100 None:intergenic
GCAGCACTGGCTTCTTTCCA+GGG 0.547988 8:-76922929 None:intergenic
TGGGAATGGAAGCGCTGCGT+TGG 0.548811 8:-76922108 None:intergenic
ATTCATTGTCTGATCCAGAC+GGG 0.548969 8:+76924151 MsaT045781.1:CDS
CCATCATTTACAGCTTTGCA+AGG 0.550593 8:-76924552 None:intergenic
AAGATGTTGGAAACTGACTT+GGG 0.551013 8:+76923937 MsaT045781.1:CDS
TGGGATTGCAAAGTACTGCT+GGG 0.552611 8:+76925494 MsaT045781.1:CDS
CCAACATTTCCCTCTTGGAT+GGG 0.553722 8:-76927355 None:intergenic
AAATGATGGTCTATGGCTGC+AGG 0.554515 8:+76924566 MsaT045781.1:CDS
ATTGTTATCATCGATTTGCA+GGG 0.554872 8:-76922960 None:intergenic
GGAACCCCTTTGCTTTCATG+GGG 0.556059 8:+76924366 MsaT045781.1:CDS
ACATACAGAGCTAACACAAA+GGG 0.557241 8:+76925462 MsaT045781.1:CDS
CTGAAGGTCGAGAAAGGAAG+CGG 0.561497 8:-76923074 None:intergenic
GTATGTTGACTCGCGTGAAA+AGG 0.562047 8:+76927210 MsaT045781.1:CDS
TGGCTGCACTAACAAGATGT+TGG 0.562461 8:+76923924 MsaT045781.1:CDS
ATTGAAGGGCCTTCTGAGGA+AGG 0.562708 8:+76924234 MsaT045781.1:CDS
CCTCTGTCAAAGTGGCGGCA+TGG 0.563399 8:+76923474 MsaT045781.1:CDS
ACATACCTGAAGGTCGAGAA+AGG 0.565685 8:-76923080 None:intergenic
CCTCTAACATGTACCCGTAC+AGG 0.568599 8:-76925202 None:intergenic
CAGGATTGTTTCTTCACCGT+CGG 0.568921 8:+76926878 MsaT045781.1:intron
GAGACGATGAAGGTGATAAG+TGG 0.569640 8:-76922284 None:intergenic
CGAGAAAGGAAGCGGCAGCA+AGG 0.571828 8:-76923066 None:intergenic
CAGAGATAACTGATGTGCAC+AGG 0.572463 8:+76926905 MsaT045781.1:CDS
ATTCTCCCATTCTATACCAA+TGG 0.575296 8:-76925569 None:intergenic
TAACCTGAAATATGAACTGC+GGG 0.576242 8:-76922314 None:intergenic
GGTGTCTCTAACAATTGTAC+GGG 0.576585 8:-76925538 None:intergenic
ACATTTCTGATTCCAATCAA+CGG 0.579154 8:+76924049 MsaT045781.1:CDS
GGGAGAGGATGAGACGATGA+AGG 0.579393 8:-76922294 None:intergenic
TTCAGATAACAACAATAGGT+TGG 0.581192 8:+76924269 MsaT045781.1:CDS
AACTACATTTGTTCCTGCAG+CGG 0.583833 8:+76925258 MsaT045781.1:CDS
TGGCCATATAGGCAGAAGAC+AGG 0.584793 8:+76924389 MsaT045781.1:CDS
TCATCATTGGCTGCAGACTG+AGG 0.584853 8:-76925343 None:intergenic
TAGCAGGATCCTTCCTCAGA+AGG 0.587745 8:-76924243 None:intergenic
CTTCTGGAGAATGCTTGACC+TGG 0.589008 8:-76926598 None:intergenic
ATTTCTTAATATATTTGAAG+GGG 0.591640 8:+76924455 MsaT045781.1:CDS
CAAGGGTGCGCCGTCCCTGG+GGG 0.593226 8:-76926766 None:intergenic
CATCATTGGCTGCAGACTGA+GGG 0.595380 8:-76925342 None:intergenic
TACTGACCTCTAATGATCTG+TGG 0.595873 8:+76923655 MsaT045781.1:CDS
GCTTTAGTCGGGCTTAGCCA+GGG 0.596401 8:+76925418 MsaT045781.1:CDS
TTGGATCATTGTTATCGGAA+TGG 0.599637 8:-76922147 None:intergenic
AATTTGAACATGTTCGCAGA+GGG 0.601296 8:-76922209 None:intergenic
TGGCCACCCCATGAAAGCAA+AGG 0.602137 8:-76924372 None:intergenic
ATTGTTTCTTCACCGTCGGA+AGG 0.603193 8:+76926882 MsaT045781.1:CDS
GGGCTTGCAGGTATTGGCCG+TGG 0.604369 8:+76925235 MsaT045781.1:CDS
CCTAAAGCTCCTAAAGGATG+GGG 0.605954 8:+76927413 MsaT045781.1:CDS
TTCTTGTATGTAAGATCCGG+TGG 0.606895 8:-76925095 None:intergenic
AAGGGAAAAGGTATAGCTGA+TGG 0.608328 8:+76923904 MsaT045781.1:CDS
TTTCTTAATATATTTGAAGG+GGG 0.609043 8:+76924456 MsaT045781.1:CDS
TCCATATCACAAGGATACGA+TGG 0.609813 8:-76924111 None:intergenic
TTATCGGAATGGTGAGATGA+TGG 0.610701 8:-76922136 None:intergenic
CAATTTGAACATGTTCGCAG+AGG 0.611329 8:-76922210 None:intergenic
TCGTATCCTTGTGATATGGA+AGG 0.611508 8:+76924114 MsaT045781.1:CDS
CTGCGTTGGTGATAGGGTGA+AGG 0.611633 8:-76922094 None:intergenic
TAGTCGGGCTTAGCCAGGGA+GGG 0.611766 8:+76925422 MsaT045781.1:CDS
CCATGCCGCCACTTTGACAG+AGG 0.611997 8:-76923474 None:intergenic
GAAAAGACAAATCGTGTAGG+AGG 0.612265 8:-76925286 None:intergenic
AAGCTCCTAAAGGATGGGGT+TGG 0.613769 8:+76927417 MsaT045781.1:CDS
GAGACACCAGAACATACCAT+TGG 0.615183 8:+76925553 MsaT045781.1:CDS
CGTTGGTGATAGGGTGAAGG+AGG 0.615421 8:-76922091 None:intergenic
ACTTACCCAAAGCGAAAAGG+GGG 0.617208 8:-76925643 None:intergenic
GGGTATTGAAGGGCCTTCTG+AGG 0.620419 8:+76924230 MsaT045781.1:CDS
GGGCTCACTTCCAGTGAATG+GGG 0.620580 8:+76924336 MsaT045781.1:CDS
CAGTTATCTCTGCCTTCCGA+CGG 0.621554 8:-76926894 None:intergenic
AATTCATTGTCTGATCCAGA+CGG 0.623772 8:+76924150 MsaT045781.1:CDS
CGCGTGAAAAGGTGACTGCA+CGG 0.624156 8:+76927221 MsaT045781.1:CDS
CAGGAACAAATGTAGTTCCA+CGG 0.627613 8:-76925252 None:intergenic
TATTATCATTAAGTTTAGGG+TGG 0.630240 8:-76923030 None:intergenic
GGGATTGCAAAGTACTGCTG+GGG 0.630860 8:+76925495 MsaT045781.1:CDS
ATAATCATAACAATATACCT+GGG 0.635196 8:+76925119 MsaT045781.1:CDS
CTACTGGAGTGGGTATTGAA+GGG 0.635325 8:+76924220 MsaT045781.1:CDS
TAATCATAACAATATACCTG+GGG 0.636900 8:+76925120 MsaT045781.1:CDS
AAGATTGAACATCCTGCACA+TGG 0.639055 8:+76923835 MsaT045781.1:CDS
TGCTGGAACAACTACTGGAG+TGG 0.639082 8:+76924209 MsaT045781.1:CDS
GTTGACTTGCCCATCCAAGA+GGG 0.642821 8:+76927346 MsaT045781.1:intron
GACTTACCCAAAGCGAAAAG+GGG 0.644219 8:-76925644 None:intergenic
GATAACTGATGTGCACAGGA+AGG 0.644413 8:+76926909 MsaT045781.1:CDS
CATGTTCGCAGAGGGAAGCG+AGG 0.645597 8:-76922201 None:intergenic
GCTGGAACAACTACTGGAGT+GGG 0.645613 8:+76924210 MsaT045781.1:CDS
TGGCGGCATGGAGTTGAGAG+AGG 0.646346 8:+76923486 MsaT045781.1:CDS
CGAGAAAAGACAAATCGTGT+AGG 0.648943 8:-76925289 None:intergenic
AGTTGACTTGCCCATCCAAG+AGG 0.651148 8:+76927345 MsaT045781.1:intron
ACTTACCGCTTCTCTTCACA+GGG 0.651417 8:-76923681 None:intergenic
GAAGTGCACAGGCTTGGTGA+TGG 0.655070 8:+76926575 MsaT045781.1:CDS
GGATACCCTGTGAAGAGAAG+CGG 0.660763 8:+76923676 MsaT045781.1:CDS
GGCTACCTCTGTCAAAGTGG+CGG 0.660938 8:+76923469 MsaT045781.1:CDS
GTTCCCATAAAGATATGCCA+AGG 0.664821 8:-76923008 None:intergenic
ATTCATTCTGATAACACCAA+GGG 0.665141 8:+76923886 MsaT045781.1:CDS
CATTCATTCTGATAACACCA+AGG 0.669271 8:+76923885 MsaT045781.1:CDS
GAAGCACGTGCTGATCACAA+TGG 0.669347 8:+76925370 MsaT045781.1:CDS
TTAGTCGGGCTTAGCCAGGG+AGG 0.669348 8:+76925421 MsaT045781.1:CDS
TGGGGCTACCTCTGTCAAAG+TGG 0.670960 8:+76923466 MsaT045781.1:CDS
CGTGTTAGAACTGCTTGTTG+GGG 0.673137 8:+76923448 MsaT045781.1:CDS
TACATACAGAGCTAACACAA+AGG 0.673898 8:+76925461 MsaT045781.1:CDS
TTCATTGTCTGATCCAGACG+GGG 0.678647 8:+76924152 MsaT045781.1:CDS
GAAGGTCCTTCCATATCACA+AGG 0.679216 8:-76924120 None:intergenic
GAGAACATCATGTTATGTTG+AGG 0.681319 8:+76924176 MsaT045781.1:CDS
GAGAGCACGTAATTCAGCGG+TGG 0.682747 8:-76922170 None:intergenic
ATCGAGAGCACGTAATTCAG+CGG 0.684133 8:-76922173 None:intergenic
TGATAACAATGTTAATGACG+AGG 0.687261 8:+76922973 MsaT045781.1:CDS
GTAGTTGTTCCAGCACCAAG+AGG 0.688597 8:-76924201 None:intergenic
AGATGTTGGAAACTGACTTG+GGG 0.689189 8:+76923938 MsaT045781.1:CDS
CTTACCCAAAGCGAAAAGGG+GGG 0.699863 8:-76925642 None:intergenic
CCAAGATTATGGCATTCATG+GGG 0.706870 8:+76923423 MsaT045781.1:CDS
CAGGTCAAAGCTCATCCCAG+AGG 0.707800 8:+76924408 MsaT045781.1:CDS
GGATTGCAAAGTACTGCTGG+GGG 0.721700 8:+76925496 MsaT045781.1:CDS
AATCATAACAATATACCTGG+GGG 0.723850 8:+76925121 MsaT045781.1:CDS
ATAACCTGAAATATGAACTG+CGG 0.723985 8:-76922315 None:intergenic
GCCACCCCATGAAAGCAAAG+GGG 0.742348 8:-76924370 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 76922058 76927523 76922058 ID=MsaG045781
Chr8 mRNA 76922058 76927523 76922058 ID=MsaT045781.1;Parent=MsaG045781
Chr8 exon 76922058 76922332 76922058 ID=MsaT045781.1.exon1;Parent=MsaT045781.1
Chr8 CDS 76922058 76922332 76922058 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76922913 76923096 76922913 ID=MsaT045781.1.exon2;Parent=MsaT045781.1
Chr8 CDS 76922913 76923096 76922913 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76923240 76923308 76923240 ID=MsaT045781.1.exon3;Parent=MsaT045781.1
Chr8 CDS 76923240 76923308 76923240 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76923409 76923507 76923409 ID=MsaT045781.1.exon4;Parent=MsaT045781.1
Chr8 CDS 76923409 76923507 76923409 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76923618 76923697 76923618 ID=MsaT045781.1.exon5;Parent=MsaT045781.1
Chr8 CDS 76923618 76923697 76923618 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76923789 76924587 76923789 ID=MsaT045781.1.exon6;Parent=MsaT045781.1
Chr8 CDS 76923789 76924587 76923789 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76924983 76925659 76924983 ID=MsaT045781.1.exon7;Parent=MsaT045781.1
Chr8 CDS 76924983 76925659 76924983 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76926562 76926646 76926562 ID=MsaT045781.1.exon8;Parent=MsaT045781.1
Chr8 CDS 76926562 76926646 76926562 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76926737 76926788 76926737 ID=MsaT045781.1.exon9;Parent=MsaT045781.1
Chr8 CDS 76926737 76926788 76926737 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76926881 76926930 76926881 ID=MsaT045781.1.exon10;Parent=MsaT045781.1
Chr8 CDS 76926881 76926930 76926881 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76927202 76927249 76927202 ID=MsaT045781.1.exon11;Parent=MsaT045781.1
Chr8 CDS 76927202 76927249 76927202 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Chr8 exon 76927347 76927523 76927347 ID=MsaT045781.1.exon12;Parent=MsaT045781.1
Chr8 CDS 76927347 76927523 76927347 ID=cds.MsaT045781.1;Parent=MsaT045781.1
Gene Sequence

>MsaT045781.1

ATGATGGAGAACCCTCAATATCCAAACAACCCTCCTCCTTCACCCTATCACCAACGCAGCGCTTCCATTCCCAAGCAGCCATCATCTCACCATTCCGATAACAATGATCCAACCACCGCTGAATTACGTGCTCTCGATTGTAACCTCGCTTCCCTCTGCGAACATGTTCAAATTGAAGGTTTCAATTCCGGCTCTTTTTCCGATATAGTTGTTAATGCAATGGGTTCCACTTATCACCTTCATCGTCTCATCCTCTCCCGCAGTTCATATTTCAGGAACATGCTTCATGGTCCCTGGAAAGAAGCCAGTGCTGCTATTGTGACCCTGCAAATCGATGATAACAATGTTAATGACGAGGCTATTGCAATTGCCTTGGCATATCTTTATGGGAACCACCCTAAACTTAATGATAATAATGCATTTCGTGTCCTTGCTGCCGCTTCCTTTCTCGACCTTCAGGATTTATGTGCTATATGTACAGATTTCATTATATCTGAGCTATGGACTTCAAACTTCTTGGCTTATCAGGTGTTTGCGGAGAACCAAGATTATGGCATTCATGGGGAGCGTGTTAGAACTGCTTGTTGGGGCTACCTCTGTCAAAGTGGCGGCATGGAGTTGAGAGAGGTGCTTCCTAAACTTTCATCTCATACATTACATGCATTACTGACCTCTAATGATCTGTGGATACCCTGTGAAGAGAAGCGGTTTGAATTGGCTTTCCACACATTCCTGGCAAAAAGTGCTCACTGCAAGATTGAACATCCTGCACATGGAATTCCTGGTTCTGAGTCTGGAACTGGCATTCATTCTGATAACACCAAGGGAAAAGGTATAGCTGATGGCTGCACTAACAAGATGTTGGAAACTGACTTGGGGAAAATGAGTCTTAAAAGTGATCTAAAGGACACTAGCATGCCTAACCTTTTAGTTGAGCTTGGAGATTCAGAAGGAGACTTCAACAGTGACATTTCTGATTCCAATCAACGGGTTCAACTTGCTTCATATGCCATTTCACCAAACTTGAATCCATCGTATCCTTGTGATATGGAAGGACCTTCATTGGGTAATTCATTGTCTGATCCAGACGGGGTGAGAACATCATGTTATGTTGAGGTGCCTCTTGGTGCTGGAACAACTACTGGAGTGGGTATTGAAGGGCCTTCTGAGGAAGGATCCTGCTACCATTCAGATAACAACAATAGGTTGGTCAGAGATCAATCAAGAGATTCTTTTTCATCGAGTTCTTGTAGTGGGCTCACTTCCAGTGAATGGGGAAGATATGGAACCCCTTTGCTTTCATGGGGTGGCCATATAGGCAGAAGACAGGTCAAAGCTCATCCCAGAGGAAACTATAGAGATGAAGACGATGTATTTCTTAATATATTTGAAGGGGGCTCACTTTTATATTGCAATATGTCTTTTGATGCACTTTTAAATGTCAGAAAGCAGCTTGAAGAGATAGGATTTCCTTGCAAAGCTGTAAATGATGGTCTATGGCTGCAGATGCTTCTTAGTCAGAGGGTACAAGAGATTGCTGCTGATACATGCAGAGTTTGTTCTCTTATGACTATGTCTTGTACTTGCCATAAGCAGTTTGCATTTTTACATGGATCTACCACCGGATCTTACATACAAGAACATAATCATAACAATATACCTGGGGGTGGTGGAAATATATATGTTGCTGAATCTTCTACAGGTGAAAGAAATGGCTCTTTTAGACCTGTACGGGTACATGTTAGAGGTGCTATTGATGGGCTTGCAGGTATTGGCCGTGGAACTACATTTGTTCCTGCAGCGGCTTCACCTCCTACACGATTTGTCTTTTCTCGTGTGCCATTTGGCGTTGGCAACAGAAACTACCCTCAGTCTGCAGCCAATGATGATTCAGAAGCACGTGCTGATCACAATGGAGACCTGTCTGGAGATGGATTGACAGCTTTAGTCGGGCTTAGCCAGGGAGGGAATTATGGAACAAATGTACATACAGAGCTAACACAAAGGGAACAAGAAATGGGATTGCAAAGTACTGCTGGGGGTGCAAGTACTGGTGGTATTCCCGTACAATTGTTAGAGACACCAGAACATACCATTGGTATAGAATGGGAGAATGACAACAGTTCTTCTATATCATTGGATTTGAAAACACCTTTAAGCCATTTCCCCCCTTTTCGCTTTGGTGTAAGTTTTGAGGAAGTGCACAGGCTTGGTGATGGCCAGGTCAAGCATTCTCCAGAAGTTTTCTATGCTGGTTCTTTGTGGAAGGTCAGTATTCAGGCTTTTAATGATGAAGACCCCCAGGGACGGCGCACCCTTGGATTGTTTCTTCACCGTCGGAAGGCAGAGATAACTGATGTGCACAGGAAGGTACATATGTATGTTGACTCGCGTGAAAAGGTGACTGCACGGTATCAGTTGACTTGCCCATCCAAGAGGGAAATGTTGGTGTTCGGAAGCTTTAAACAAACGGGCACTCTTCTACCTAAAGCTCCTAAAGGATGGGGTTGGCGAACTGCTCTACTATTTGATGAGCTTGCTGATCTTCTACAAAATGGAGCCCTAAGAGTTATTGCTGTAGTGCAGCTTGTTTGA
Protein sequence

>MsaT045781.1

MMENPQYPNNPPPSPYHQRSASIPKQPSSHHSDNNDPTTAELRALDCNLASLCEHVQIEGFNSGSFSDIVVNAMGSTYHLHRLILSRSSYFRNMLHGPWKEASAAIVTLQIDDNNVNDEAIAIALAYLYGNHPKLNDNNAFRVLAAASFLDLQDLCAICTDFIISELWTSNFLAYQVFAENQDYGIHGERVRTACWGYLCQSGGMELREVLPKLSSHTLHALLTSNDLWIPCEEKRFELAFHTFLAKSAHCKIEHPAHGIPGSESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLKSDLKDTSMPNLLVELGDSEGDFNSDISDSNQRVQLASYAISPNLNPSYPCDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGTTTGVGIEGPSEEGSCYHSDNNNRLVRDQSRDSFSSSSCSGLTSSEWGRYGTPLLSWGGHIGRRQVKAHPRGNYRDEDDVFLNIFEGGSLLYCNMSFDALLNVRKQLEEIGFPCKAVNDGLWLQMLLSQRVQEIAADTCRVCSLMTMSCTCHKQFAFLHGSTTGSYIQEHNHNNIPGGGGNIYVAESSTGERNGSFRPVRVHVRGAIDGLAGIGRGTTFVPAAASPPTRFVFSRVPFGVGNRNYPQSAANDDSEARADHNGDLSGDGLTALVGLSQGGNYGTNVHTELTQREQEMGLQSTAGGASTGGIPVQLLETPEHTIGIEWENDNSSSISLDLKTPLSHFPPFRFGVSFEEVHRLGDGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTLGLFLHRRKAEITDVHRKVHMYVDSREKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQNGALRVIAVVQLV*