AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045803

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045803 MtrunA17_Chr4g0067671 69.505 364 101 4 48 410 1 355 2.08e-177 499
MsaG045803 MtrunA17_Chr4g0067141 66.842 380 116 4 48 426 1 371 5.59e-169 478
MsaG045803 MtrunA17_Chr4g0067121 49.091 330 165 2 97 425 51 378 5.23e-107 321
MsaG045803 MtrunA17_Chr2g0295731 46.910 356 180 3 75 428 30 378 1.39e-105 317
MsaG045803 MtrunA17_Chr2g0296121 48.477 394 186 7 41 425 69 454 7.17e-105 318
MsaG045803 MtrunA17_Chr2g0295761 48.955 335 168 2 91 424 54 386 2.12e-103 312
MsaG045803 MtrunA17_Chr2g0296721 47.564 349 177 3 80 424 33 379 1.79e-101 308
MsaG045803 MtrunA17_Chr2g0296251 45.195 385 168 5 79 425 17 396 3.93e-101 306
MsaG045803 MtrunA17_Chr2g0296741 46.243 346 179 3 85 425 58 401 1.06e-100 306
MsaG045803 MtrunA17_Chr2g0296301 45.133 339 184 1 87 425 28 364 4.56e-100 303
MsaG045803 MtrunA17_Chr2g0315731 46.286 350 181 3 80 424 53 400 1.77e-98 301
MsaG045803 MtrunA17_Chr2g0296111 45.869 351 182 3 77 425 24 368 1.61e-97 297
MsaG045803 MtrunA17_Chr2g0296221 47.399 346 173 4 87 425 69 412 3.00e-97 298
MsaG045803 MtrunA17_Chr2g0296711 46.687 332 170 3 99 425 55 384 5.53e-97 297
MsaG045803 MtrunA17_Chr2g0296151 43.947 380 199 4 48 425 1 368 3.04e-96 293
MsaG045803 MtrunA17_Chr2g0296191 43.864 383 199 4 46 428 145 511 9.35e-95 306
MsaG045803 MtrunA17_Chr2g0296191 47.851 349 171 5 87 428 652 996 2.29e-92 300
MsaG045803 MtrunA17_Chr2g0296171 46.328 354 178 5 82 425 42 393 3.87e-94 289
MsaG045803 MtrunA17_Chr2g0295741 43.636 385 199 6 48 425 1 374 2.19e-93 286
MsaG045803 MtrunA17_Chr2g0296231 45.232 367 182 6 66 424 22 377 4.32e-93 286
MsaG045803 MtrunA17_Chr2g0296291 41.206 398 186 5 74 425 1 396 4.93e-92 284
MsaG045803 MtrunA17_Chr2g0286191 45.833 336 166 4 97 425 31 357 2.17e-91 280
MsaG045803 MtrunA17_Chr2g0296181 42.733 344 155 7 91 425 45 355 1.34e-79 251
MsaG045803 MtrunA17_Chr4g0001831 33.634 333 214 3 98 425 38 368 9.72e-61 201
MsaG045803 MtrunA17_Chr2g0296131 54.545 154 70 0 272 425 7 160 8.84e-56 182
MsaG045803 MtrunA17_Chr2g0296551 47.826 184 94 1 242 425 1 182 1.73e-53 177
MsaG045803 MtrunA17_Chr2g0296161 53.247 154 72 0 272 425 3 156 1.86e-50 168
MsaG045803 MtrunA17_Chr2g0296701 51.316 152 74 0 274 425 3 154 3.80e-48 162
MsaG045803 MtrunA17_Chr2g0296211 49.708 171 84 1 255 425 188 356 8.97e-48 167
MsaG045803 MtrunA17_Chr2g0296211 43.820 89 48 1 87 173 107 195 5.42e-14 73.2
MsaG045803 MtrunA17_Chr2g0286221 48.077 156 79 2 270 425 14 167 2.30e-41 145
MsaG045803 MtrunA17_Chr8g0377781 27.596 337 242 2 92 426 37 373 1.47e-38 143
MsaG045803 MtrunA17_Chr2g0296751 50.394 127 63 0 299 425 1 127 4.00e-38 135
MsaG045803 MtrunA17_Chr8g0377801 27.353 340 243 4 92 428 50 388 1.11e-36 138
MsaG045803 MtrunA17_Chr4g0067131 69.767 86 26 0 343 428 1 86 1.66e-35 126
MsaG045803 MtrunA17_Chr2g0295711 39.103 156 93 1 91 244 40 195 3.31e-32 121
MsaG045803 MtrunA17_Chr4g0067681 86.885 61 8 0 332 392 1 61 4.67e-32 116
MsaG045803 MtrunA17_Chr2g0295721 51.282 117 57 0 272 388 3 119 6.89e-32 117
MsaG045803 MtrunA17_Chr2g0296561 48.819 127 58 1 77 203 23 142 6.59e-26 102
MsaG045803 MtrunA17_Chr2g0295751 39.865 148 82 2 79 224 34 176 2.64e-25 107
MsaG045803 MtrunA17_Chr2g0294671 24.632 272 185 6 134 394 215 477 1.28e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045803 AT5G07900.1 32.113 355 233 3 80 426 47 401 6.44e-66 216
MsaG045803 AT1G61990.2 30.952 420 228 10 57 427 5 411 9.21e-48 169
MsaG045803 AT1G61990.1 30.952 420 228 10 57 427 5 411 9.21e-48 169
MsaG045803 AT1G21150.1 30.514 331 224 3 97 422 55 384 2.11e-47 167
MsaG045803 AT1G21150.2 30.514 331 224 3 97 422 119 448 8.88e-47 167
MsaG045803 AT1G21150.3 29.429 333 229 3 95 422 17 348 3.13e-45 160
MsaG045803 AT1G61970.2 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61970.7 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61970.6 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61970.3 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61970.5 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61970.4 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61970.1 27.163 416 254 11 57 426 5 417 2.79e-42 154
MsaG045803 AT1G61980.3 27.371 369 227 7 97 426 51 417 2.11e-41 152
MsaG045803 AT1G61980.2 27.371 369 227 7 97 426 51 417 2.11e-41 152
MsaG045803 AT1G61980.1 27.371 369 227 7 97 426 51 417 2.11e-41 152
MsaG045803 AT1G62120.1 25.954 393 245 7 79 426 32 423 1.58e-39 147
MsaG045803 AT5G64950.1 29.086 361 240 5 78 428 25 379 6.67e-34 131
MsaG045803 AT1G62085.1 30.952 294 191 4 57 339 5 297 1.79e-33 131
MsaG045803 AT1G62085.2 30.952 294 191 4 57 339 5 297 1.79e-33 131
MsaG045803 AT1G62150.1 24.623 398 225 7 93 416 52 448 1.42e-31 125
MsaG045803 AT1G62110.1 23.598 428 248 9 78 427 32 458 1.44e-31 125
MsaG045803 AT3G46950.1 23.430 414 249 6 81 427 35 447 1.74e-31 125
MsaG045803 AT5G23930.1 23.832 428 250 9 72 426 23 447 3.72e-29 119
MsaG045803 AT1G62010.1 24.576 354 218 8 114 426 56 401 2.23e-28 116
MsaG045803 AT1G61960.1 28.409 264 185 3 78 339 32 293 1.89e-27 114
MsaG045803 AT1G61960.1 27.692 195 135 3 236 426 261 453 2.28e-15 78.2
MsaG045803 AT1G56380.2 22.740 365 239 8 89 411 17 380 4.04e-21 95.1
MsaG045803 AT1G56380.1 22.740 365 239 8 89 411 6 369 4.20e-21 94.7

Find 82 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGAAGGTAACAGCATTT+TGG 0.187021 8:-77130103 None:intergenic
AAATTGAAACTTTGGCAAAA+TGG 0.240171 8:-77130398 None:intergenic
GAAGGTTGTGGAGGAGATAA+AGG 0.240813 8:+77130717 MsaT045803.1:CDS
TGGAGGAGATAAAGGGATTA+GGG 0.268808 8:+77130725 MsaT045803.1:CDS
TGTGGCATTACGGGCTAAAA+TGG 0.284762 8:+77130774 MsaT045803.1:CDS
TACACTGTTGAGGCTATATC+AGG 0.297863 8:+77131158 MsaT045803.1:CDS
ATACTAAATCCGAAAATATC+TGG 0.310676 8:-77130985 None:intergenic
AACTCATCAGTCAAAGAAAA+TGG 0.332076 8:-77131093 None:intergenic
GATGAAAGAAGACGTTAATA+TGG 0.332378 8:+77124941 MsaT045803.1:CDS
GTGGAGGAGATAAAGGGATT+AGG 0.373434 8:+77130724 MsaT045803.1:CDS
GGTGGTGGTGGTGGAGAAGT+TGG 0.386707 8:-77130149 None:intergenic
GTCTTGAGGAGAAGCACCTT+TGG 0.388823 8:-77130428 None:intergenic
AGTTGATGCCTTCAAGAGTT+GGG 0.390941 8:+77130825 MsaT045803.1:CDS
ACTCTTCTTCATCAAACCTT+TGG 0.405522 8:-77131055 None:intergenic
AAGGTTGTGGAGGAGATAAA+GGG 0.406206 8:+77130718 MsaT045803.1:CDS
CTTTGGCAAAATGGTTTCGA+TGG 0.432342 8:-77130389 None:intergenic
CTAGCTTTATGCTACGGTCT+AGG 0.434831 8:+77130890 MsaT045803.1:CDS
TTGGGTCAAACAGCATGGTT+GGG 0.439509 8:+77130939 MsaT045803.1:CDS
TCAGATATACAGAATATAGA+AGG 0.443327 8:-77130682 None:intergenic
AACCTTTGGAAAGAAGATAC+TGG 0.447858 8:-77131041 None:intergenic
AGATAAAAGAATCGGGTTTG+TGG 0.450214 8:-77130285 None:intergenic
TTTATCTGAATCTGAAGAAA+TGG 0.460894 8:-77130170 None:intergenic
GGGTTTGGGGAAGATCGTTA+AGG 0.463136 8:-77130081 None:intergenic
GTCCAGTATCTTCTTTCCAA+AGG 0.465341 8:+77131039 MsaT045803.1:CDS
TGAAGAAATGGTGGTGGTGG+TGG 0.467625 8:-77130158 None:intergenic
AGAACAACCCCAACTCTTGA+AGG 0.470291 8:-77130833 None:intergenic
GGCTATCTCCAAATCCCATT+GGG 0.476729 8:+77130795 MsaT045803.1:CDS
TCGGATTTAGTATAGAGAAA+AGG 0.481241 8:+77130995 MsaT045803.1:CDS
GAAGTTGGAACTGAAAGAAT+GGG 0.483346 8:-77130134 None:intergenic
TTTGGGTCAAACAGCATGGT+TGG 0.487146 8:+77130938 MsaT045803.1:CDS
GATAAAAGAATCGGGTTTGT+GGG 0.492825 8:-77130284 None:intergenic
CTTCTTACCAAATCAAAAGC+AGG 0.493927 8:-77130514 None:intergenic
AGAAGTTGGAACTGAAAGAA+TGG 0.498846 8:-77130135 None:intergenic
CGAGATCTAGCAATTGAAGT+TGG 0.500150 8:-77130583 None:intergenic
AAGTTGATGCCTTCAAGAGT+TGG 0.504718 8:+77130824 MsaT045803.1:CDS
AGCAAATAGTAAATACTTGA+GGG 0.506552 8:-77130649 None:intergenic
CAGGGATTATTTGTTTGTCA+AGG 0.513847 8:-77130495 None:intergenic
CTGATGAGTTGTTCCTGAAA+AGG 0.516369 8:+77131106 MsaT045803.1:CDS
GTTTGTCAAGGCTTGAACAA+AGG 0.516951 8:-77130483 None:intergenic
TAGCTTTATGCTACGGTCTA+GGG 0.517977 8:+77130891 MsaT045803.1:CDS
TGGCTATCTCCAAATCCCAT+TGG 0.520598 8:+77130794 MsaT045803.1:CDS
TCATACCTCATTAACAATTG+TGG 0.523901 8:+77130208 MsaT045803.1:CDS
ATAAACCACAATTGTTAATG+AGG 0.532392 8:-77130213 None:intergenic
TTTGGCAAAATGGTTTCGAT+GGG 0.532889 8:-77130388 None:intergenic
TTAGGGTTTCACCCTTCCAA+TGG 0.533513 8:+77130742 MsaT045803.1:CDS
TATAGTAATAGCTCTTTGAT+CGG 0.537722 8:-77130548 None:intergenic
AATGGGGTCGGAGAAGAAGA+AGG 0.539197 8:-77130117 None:intergenic
TAAAAGAATCGGGTTTGTGG+GGG 0.539770 8:-77130282 None:intergenic
GATGGGATTTGAAACGATGA+TGG 0.544819 8:-77130371 None:intergenic
GATGGTTTGTATCTGATCGT+TGG 0.547224 8:-77130332 None:intergenic
GAAAACCTAGCTTTATGCTA+CGG 0.549131 8:+77130884 MsaT045803.1:CDS
TAGAGAAAAGGCTTATTCCA+AGG 0.551644 8:+77131007 MsaT045803.1:CDS
AGACAACAATGGAGGAGCAT+AGG 0.554160 8:+77124901 None:intergenic
TACTGGACAACAGAAGCCCT+TGG 0.554999 8:-77131024 None:intergenic
GTAATAGCTCTTTGATCGGA+AGG 0.557454 8:-77130544 None:intergenic
AAGCAAATAGTAAATACTTG+AGG 0.559219 8:-77130650 None:intergenic
GGGTGGAATCCTGCGAATGA+TGG 0.561606 8:-77130350 None:intergenic
GTTGATGCCTTCAAGAGTTG+GGG 0.563843 8:+77130826 MsaT045803.1:CDS
GGAGCTTTCAAGAGCACGAA+AGG 0.567930 8:-77130964 None:intergenic
ATAAAAGAATCGGGTTTGTG+GGG 0.573357 8:-77130283 None:intergenic
ATCTGAAGAAATGGTGGTGG+TGG 0.576685 8:-77130161 None:intergenic
TTCTTACCAAATCAAAAGCA+GGG 0.579986 8:-77130513 None:intergenic
CTAGACCGTAGCATAAAGCT+AGG 0.580960 8:-77130889 None:intergenic
AGATACAAACCATCATTCGC+AGG 0.582170 8:+77130341 MsaT045803.1:CDS
AGAGAAAAGGCTTATTCCAA+GGG 0.584737 8:+77131008 MsaT045803.1:CDS
GGCTTGAACAAAGGAAACGA+GGG 0.588494 8:-77130474 None:intergenic
AAAAGAATCGGGTTTGTGGG+GGG 0.600161 8:-77130281 None:intergenic
GAAGCTTTGAGAGCATCTTG+TGG 0.608539 8:-77130238 None:intergenic
TGGAACTGAAAGAATGGGGT+CGG 0.610694 8:-77130129 None:intergenic
AAGATTCAACGAAATTATCA+CGG 0.610977 8:-77130015 None:intergenic
AGGAAGCATCTACACTGTTG+AGG 0.632213 8:+77131148 MsaT045803.1:CDS
ATGACAGTGAAACATTGAGA+AGG 0.633930 8:-77130038 None:intergenic
ATGGGATTTGAAACGATGAT+GGG 0.634429 8:-77130370 None:intergenic
ATCTGAATCTGAAGAAATGG+TGG 0.644416 8:-77130167 None:intergenic
TGAATCTGAAGAAATGGTGG+TGG 0.646777 8:-77130164 None:intergenic
GGATTTGAAACGATGATGGG+TGG 0.649645 8:-77130367 None:intergenic
AGGCTTGAACAAAGGAAACG+AGG 0.672222 8:-77130475 None:intergenic
ACACTGTTGAGGCTATATCA+GGG 0.672459 8:+77131159 MsaT045803.1:CDS
AAGTTGGAACTGAAAGAATG+GGG 0.707727 8:-77130133 None:intergenic
ACAGTGACAACTATGTCTTG+AGG 0.750188 8:-77130442 None:intergenic
GTCTAGGGACAAACTCAATG+CGG 0.752501 8:+77130906 MsaT045803.1:CDS
CTGTTGAGGCTATATCAGGG+AGG 0.767499 8:+77131162 MsaT045803.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 77124909 77131191 77124909 ID=MsaG045803
Chr8 mRNA 77124909 77131191 77124909 ID=MsaT045803.1;Parent=MsaG045803
Chr8 exon 77124909 77124994 77124909 ID=MsaT045803.1.exon1;Parent=MsaT045803.1
Chr8 CDS 77124909 77124994 77124909 ID=cds.MsaT045803.1;Parent=MsaT045803.1
Chr8 exon 77129991 77131191 77129991 ID=MsaT045803.1.exon2;Parent=MsaT045803.1
Chr8 CDS 77129991 77131191 77129991 ID=cds.MsaT045803.1;Parent=MsaT045803.1
Gene Sequence

>MsaT045803.1

ATGGAGGAGCATAGGATGCATAAGAAAAAGAAGATGAAAGAAGACGTTAATATGGTGAAAGCTTTAAAAGAAACACTTCTTTCAACAGTTTTGTGCATCAAAGGTATTTGCCGTGATAATTTCGTTGAATCTTCCTTCTCAATGTTTCACTGTCATCGTCTCAGAACTCTTCTCTACCTTAACGATCTTCCCCAAACCCCAAAATGCTGTTACCTTCTTCTTCTCCGACCCCATTCTTTCAGTTCCAACTTCTCCACCACCACCACCATTTCTTCAGATTCAGATAAACAATCTTTCACAGTTTCATACCTCATTAACAATTGTGGTTTATCTCCACAAGATGCTCTCAAAGCTTCTAAACGATTTCATTTCACCACCCCCCACAAACCCGATTCTTTTATCTCCTTTTTCAAAACCCACGGTTTTTCCAACGATCAGATACAAACCATCATTCGCAGGATTCCACCCATCATCGTTTCAAATCCCATCGAAACCATTTTGCCAAAGTTTCAATTTTTAGCTTCCAAAGGTGCTTCTCCTCAAGACATAGTTGTCACTGTCACTAAAAGCCCTCGTTTCCTTTGTTCAAGCCTTGACAAACAAATAATCCCTGCTTTTGATTTGGTAAGAAGTTTCTGTCCTTCCGATCAAAGAGCTATTACTATAATAAATTCTTATCCAACTTCAATTGCTAGATCTCGCATGAAACCAAATGTTCAATTTTTGCTTGATTTTGGAGTCAATCCCTCAAGTATTTACTATTTGCTTAGTAGTAGACCTTCTATATTCTGTATATCTGATTCTGATTTTAGGAAGGTTGTGGAGGAGATAAAGGGATTAGGGTTTCACCCTTCCAATGGATATTTTTGTGTGGCATTACGGGCTAAAATGGCTATCTCCAAATCCCATTGGGATGCAAAAGTTGATGCCTTCAAGAGTTGGGGTTGTTCTGAAGATGATATTTTGACTGCATTTAAAAGAAAACCTAGCTTTATGCTACGGTCTAGGGACAAACTCAATGCGGTGATGCGTTTTTGGGTCAAACAGCATGGTTGGGATCCTTTCGTGCTCTTGAAAGCTCCAGATATTTTCGGATTTAGTATAGAGAAAAGGCTTATTCCAAGGGCTTCTGTTGTCCAGTATCTTCTTTCCAAAGGTTTGATGAAGAAGAGTGCTAGCTTATATACACCATTTTCTTTGACTGATGAGTTGTTCCTGAAAAGGTATGTGAATTATTTTGAGGAGGAAGCATCTACACTGTTGAGGCTATATCAGGGAGGATGCTAG
Protein sequence

>MsaT045803.1

MEEHRMHKKKKMKEDVNMVKALKETLLSTVLCIKGICRDNFVESSFSMFHCHRLRTLLYLNDLPQTPKCCYLLLLRPHSFSSNFSTTTTISSDSDKQSFTVSYLINNCGLSPQDALKASKRFHFTTPHKPDSFISFFKTHGFSNDQIQTIIRRIPPIIVSNPIETILPKFQFLASKGASPQDIVVTVTKSPRFLCSSLDKQIIPAFDLVRSFCPSDQRAITIINSYPTSIARSRMKPNVQFLLDFGVNPSSIYYLLSSRPSIFCISDSDFRKVVEEIKGLGFHPSNGYFCVALRAKMAISKSHWDAKVDAFKSWGCSEDDILTAFKRKPSFMLRSRDKLNAVMRFWVKQHGWDPFVLLKAPDIFGFSIEKRLIPRASVVQYLLSKGLMKKSASLYTPFSLTDELFLKRYVNYFEEEASTLLRLYQGGC*