AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045843

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045843 MtrunA17_Chr4g0068161 93.434 929 31 3 1 904 1 924 0.0 1738
MsaG045843 MtrunA17_Chr2g0294711 69.647 906 237 6 1 880 1 894 0.0 1300
MsaG045843 MtrunA17_Chr8g0382191 33.061 611 365 14 300 872 433 1037 1.13e-84 294
MsaG045843 MtrunA17_Chr8g0382191 55.725 131 58 0 25 155 9 139 3.87e-41 164
MsaG045843 MtrunA17_Chr8g0377501 35.714 588 311 22 327 880 387 941 1.25e-83 289
MsaG045843 MtrunA17_Chr8g0377501 48.276 145 73 1 24 166 13 157 5.90e-39 156
MsaG045843 MtrunA17_Chr2g0279141 31.270 630 385 12 305 898 398 1015 2.49e-81 284
MsaG045843 MtrunA17_Chr2g0279141 34.713 314 150 9 25 290 13 319 1.04e-39 159
MsaG045843 MtrunA17_Chr4g0050401 31.329 632 380 17 305 897 386 1002 1.48e-79 278
MsaG045843 MtrunA17_Chr4g0050401 54.032 124 57 0 25 148 13 136 7.24e-37 150
MsaG045843 MtrunA17_Chr3g0122351 32.639 576 314 17 336 872 368 908 1.60e-73 260
MsaG045843 MtrunA17_Chr3g0122351 52.419 124 59 0 26 149 8 131 8.17e-38 153
MsaG045843 MtrunA17_Chr8g0382181 45.192 104 54 1 67 170 8 108 1.42e-22 102
MsaG045843 MtrunA17_Chr8g0382181 31.522 92 63 0 356 447 374 465 6.12e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045843 AT3G16940.1 54.279 888 332 8 9 880 7 836 0.0 940
MsaG045843 AT3G16940.3 54.095 867 324 10 30 880 1 809 0.0 902
MsaG045843 AT3G16940.2 53.376 785 297 9 9 777 7 738 0.0 801
MsaG045843 AT5G09410.1 31.539 929 549 19 25 872 16 938 2.36e-130 416
MsaG045843 AT5G09410.2 31.151 947 547 20 25 872 16 956 5.80e-127 408
MsaG045843 AT5G09410.3 30.632 950 548 20 25 872 75 1015 1.79e-121 395
MsaG045843 AT2G22300.3 34.879 539 319 10 356 877 402 925 5.21e-86 296
MsaG045843 AT2G22300.3 69.231 78 24 0 71 148 1 78 7.77e-29 124
MsaG045843 AT2G22300.2 34.879 539 319 10 356 877 460 983 1.40e-85 296
MsaG045843 AT2G22300.2 57.778 135 57 0 14 148 2 136 1.96e-44 174
MsaG045843 AT2G22300.1 34.879 539 319 10 356 877 460 983 1.40e-85 296
MsaG045843 AT2G22300.1 57.778 135 57 0 14 148 2 136 1.96e-44 174
MsaG045843 AT5G09410.4 31.949 554 335 11 356 872 287 835 1.89e-79 276
MsaG045843 AT5G64220.1 31.353 606 366 13 305 872 405 998 3.70e-76 270
MsaG045843 AT5G64220.1 48.370 184 77 2 25 193 13 193 3.36e-44 174
MsaG045843 AT5G64220.2 31.353 606 366 13 305 872 405 998 3.70e-76 270
MsaG045843 AT5G64220.2 48.370 184 77 2 25 193 13 193 3.36e-44 174
MsaG045843 AT1G67310.1 32.594 586 319 18 339 872 415 976 1.46e-65 238
MsaG045843 AT1G67310.1 47.287 129 68 0 21 149 32 160 4.08e-36 148

Find 216 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCTCTGTTTGTAACTTTA+AGG -0.008543 8:-77480951 None:intergenic
TAACATCTACGAACAAAATT+TGG 0.072421 8:-77473862 None:intergenic
CATCCATTTGTGTGCAATTT+TGG 0.104350 8:+77477627 MsaT045843.1:CDS
GGAAATAAATGATGTCTTTA+AGG 0.133359 8:-77477435 None:intergenic
CTAGATTTCCGGGATAAATT+TGG 0.166054 8:+77477694 MsaT045843.1:CDS
TTACTACCTTTGCTTGATTA+AGG 0.188541 8:-77481616 None:intergenic
ATACAATGCAGGCTGCTTTC+AGG 0.201311 8:+77481312 MsaT045843.1:intron
CTGGCTTATCACTAGATTTC+CGG 0.209797 8:+77477683 MsaT045843.1:CDS
GTAAGGTTCTCGGAATTTAC+TGG 0.269930 8:-77480836 None:intergenic
CTTGAATCTTGATAGCTTGA+TGG 0.274270 8:-77481138 None:intergenic
TTGCATTGAAAACAGATTAA+AGG 0.279124 8:+77477537 MsaT045843.1:CDS
TTGTGTCTTCAGGGAGAAAA+TGG 0.285192 8:+77480601 MsaT045843.1:intron
GCATGATCCAGTAGCTTCTA+TGG 0.288531 8:+77477280 MsaT045843.1:CDS
AATCTCTACTTGAGCTTTGA+TGG 0.290115 8:+77477206 MsaT045843.1:CDS
AGGATGGGGTCTACCGTTGC+TGG 0.297900 8:+77477156 MsaT045843.1:CDS
TGTAGTAGAGTTTGAATTTA+CGG 0.298703 8:-77475301 None:intergenic
GCTCGTGAAGCCTCAGTTCA+TGG 0.300887 8:-77475854 None:intergenic
TCAGTTGATGATTCTGCTCT+TGG 0.301849 8:+77476488 MsaT045843.1:CDS
GTAAGATTAAATACTTGTTC+TGG 0.324933 8:-77476578 None:intergenic
CACACATTAGATTGGACTTT+GGG 0.329782 8:-77477097 None:intergenic
TACTACCTTTGCTTGATTAA+GGG 0.341554 8:-77481615 None:intergenic
TTTGAATTTACGGGAGTGAT+TGG 0.343053 8:-77475291 None:intergenic
AGAAAGCGCAAGAAGATTAT+AGG 0.344611 8:+77481579 MsaT045843.1:CDS
GTTGCAACTTTGCTGTCAGC+TGG 0.345081 8:+77480623 MsaT045843.1:CDS
ACTTCTCTCGACACGTTCTT+CGG 0.345176 8:-77481525 None:intergenic
CCTCGATGTTGCGAGCATTA+TGG 0.346860 8:+77473082 MsaT045843.1:CDS
AAAGTAACAACTTGGCTGAT+AGG 0.349913 8:-77477236 None:intergenic
AAGGGGAATCAGACATGATT+TGG 0.351252 8:-77476463 None:intergenic
AACATCTACGAACAAAATTT+GGG 0.354515 8:-77473861 None:intergenic
CATCATTTATTTCCAATACT+TGG 0.356488 8:+77477444 MsaT045843.1:CDS
CTGTCAGAAGAAATAGATTC+AGG 0.364565 8:+77475351 MsaT045843.1:CDS
TTGGATGGACGGCTCTTCAT+TGG 0.365957 8:+77477713 MsaT045843.1:CDS
CACAGACCTCTTGTGTTTCA+CGG 0.368339 8:-77474048 None:intergenic
CAAGCTAAAGATGCCCTGTT+TGG 0.370069 8:+77477512 MsaT045843.1:intron
AACAATATCGTAAAATCATT+TGG 0.371099 8:+77481351 MsaT045843.1:CDS
CTTCATTGGGCAGCATATTA+CGG 0.371986 8:+77477727 MsaT045843.1:CDS
CTGGGTACCACCGCATTCTC+CGG 0.372817 8:+77477175 MsaT045843.1:CDS
GTAGTAGAGTTTGAATTTAC+GGG 0.373399 8:-77475300 None:intergenic
GCTTAATACCGAGGAAGAAT+CGG 0.378006 8:+77481442 MsaT045843.1:CDS
ATTCATTGGCTTGACATTGA+TGG 0.378984 8:-77473174 None:intergenic
GAACAAGGACGATGTGTTCC+AGG 0.379575 8:-77474021 None:intergenic
AACTTACCGGGTCAACTTGT+TGG 0.380648 8:+77472506 MsaT045843.1:CDS
GATGATGGCAAATAACTTAC+CGG 0.382750 8:+77472493 MsaT045843.1:CDS
TTAGAAAAGATGGGCACAAC+TGG 0.386039 8:+77473618 MsaT045843.1:CDS
AATTTCTGCAGATGCATTGT+TGG 0.386177 8:-77476232 None:intergenic
TTCCTTTATGCAGCTGGAAC+GGG 0.389115 8:+77481719 MsaT045843.1:CDS
CATTAAGTATTGCCAAGTAT+TGG 0.389662 8:-77477456 None:intergenic
TGGCTTATCACTAGATTTCC+GGG 0.389963 8:+77477684 MsaT045843.1:CDS
ACGAGCTTAATACACTTGAA+TGG 0.394137 8:+77475871 MsaT045843.1:CDS
GTTGGATCCGTGACCAAGTT+TGG 0.394480 8:-77480653 None:intergenic
AAGATACACTGGCAGCTTAC+CGG 0.401008 8:+77480879 MsaT045843.1:CDS
CTGCTGCATCGGCAGCTGTC+CGG 0.405371 8:-77480898 None:intergenic
TGGCTTAGCAGCTTATCTTT+CGG 0.408505 8:+77480733 MsaT045843.1:CDS
CTTCGGAACTTTAGAAAAGA+TGG 0.410510 8:+77473608 MsaT045843.1:CDS
TATTGCTCACTGCAATTGGC+TGG 0.411882 8:-77476275 None:intergenic
TTCAATGACATGAGCTTAGC+TGG 0.412581 8:+77480776 MsaT045843.1:CDS
TGTTCCTGCAGAAATTGTTC+AGG 0.416056 8:+77477136 MsaT045843.1:CDS
AGAATATTCCTTTATGCAGC+TGG 0.417506 8:+77481713 MsaT045843.1:intron
TTGCAAAGTCAGGACAGCTT+TGG 0.419514 8:+77476428 MsaT045843.1:CDS
TTCTCTTCCATAGAAGCTAC+TGG 0.423128 8:-77477287 None:intergenic
CTATGGAAGAGAAGAACAAC+TGG 0.423755 8:+77477297 MsaT045843.1:CDS
ATTCCAAAATTGCACACAAA+TGG 0.426542 8:-77477630 None:intergenic
CGTAAAATCATTTGGTCAGT+TGG 0.427825 8:+77481359 MsaT045843.1:CDS
CGAGCTTAATACACTTGAAT+GGG 0.429815 8:+77475872 MsaT045843.1:CDS
AAGCATGGCATGAATCTCAT+TGG 0.433333 8:-77473132 None:intergenic
GGGACCAAAACTGCATATGC+TGG 0.433437 8:+77475372 MsaT045843.1:CDS
GCTACTGGATCATGCAAAAC+AGG 0.435079 8:-77477272 None:intergenic
CGCTGATCTTGCTTATACGA+GGG 0.436160 8:+77480703 MsaT045843.1:CDS
TCCACTACCAGAGATGAATA+TGG 0.438348 8:-77476536 None:intergenic
CAACTTGGCTGATAGGGTTA+TGG 0.443521 8:-77477229 None:intergenic
TTCCCGTTCCAGCTGCATAA+AGG 0.443756 8:-77481721 None:intergenic
ACACACATTAGATTGGACTT+TGG 0.444268 8:-77477098 None:intergenic
TGGCTCATGATACAGTTTCA+GGG 0.447890 8:+77475264 MsaT045843.1:intron
GCGTGCTTCAGCTTCTGGGC+TGG 0.448475 8:-77480984 None:intergenic
CTGCTCCTCGGTAAGGTTCT+CGG 0.448811 8:-77480846 None:intergenic
ATACTATTGCTCACTGCAAT+TGG 0.451657 8:-77476279 None:intergenic
GCGTTGGCGTTTAAAGAGAA+AGG 0.452827 8:+77481403 MsaT045843.1:CDS
AAGTAACAACTTGGCTGATA+GGG 0.459430 8:-77477235 None:intergenic
TGTTTGACCGCAAGATGCTT+CGG 0.463262 8:+77473591 MsaT045843.1:CDS
CTTGTTGGATCAGAGATACA+TGG 0.467564 8:+77472521 MsaT045843.1:CDS
ACCACATACACACATTAGAT+TGG 0.467840 8:-77477105 None:intergenic
CTTAATACCGAGGAAGAATC+GGG 0.468527 8:+77481443 MsaT045843.1:CDS
CTTCAGACAATTAGCAATCA+AGG 0.472262 8:+77476330 MsaT045843.1:CDS
ATTAAATACTTGTTCTGGAA+GGG 0.472785 8:-77476573 None:intergenic
TTGCAACTTTGCTGTCAGCT+GGG 0.472793 8:+77480624 MsaT045843.1:CDS
ATGCAGAGGAGGAATTCTTT+CGG 0.473903 8:+77481486 MsaT045843.1:CDS
CTGCAGAAATTGTTCAGGAT+GGG 0.477098 8:+77477141 MsaT045843.1:CDS
TGGATGGACGGCTCTTCATT+GGG 0.477645 8:+77477714 MsaT045843.1:CDS
AGCTGGGGCAAAGCCAAACT+TGG 0.480001 8:+77480640 MsaT045843.1:CDS
ATCAACACCACTTGTGAGAC+GGG 0.480109 8:-77476378 None:intergenic
GACAGAATCCATGGTGCAGA+TGG 0.481590 8:-77475333 None:intergenic
TGGAAATATCAGTGGCTCAC+TGG 0.481619 8:+77480796 MsaT045843.1:CDS
TTGAAAACAGATTAAAGGAA+TGG 0.490658 8:+77477542 MsaT045843.1:CDS
TAATGAACCATATTCATCTC+TGG 0.490693 8:+77476529 MsaT045843.1:CDS
CTGATCTCCCGATTCTTCCT+CGG 0.494626 8:-77481450 None:intergenic
TACTGATGTGTCCCCTGCGT+GGG 0.495048 8:+77476598 MsaT045843.1:CDS
GTCAGGACAGCTTTGGAATG+TGG 0.495726 8:+77476435 MsaT045843.1:CDS
CACGCATACAAGAAGCATAT+AGG 0.496664 8:+77480921 MsaT045843.1:CDS
TGGGTACCACCGCATTCTCC+GGG 0.502761 8:+77477176 MsaT045843.1:CDS
AGAGGTTTATGCGATGATGA+TGG 0.505647 8:+77472478 None:intergenic
TACAACATAGATTCCGTACA+TGG 0.506906 8:+77481086 MsaT045843.1:CDS
CTTTGTCTCAAAATTGCGGA+AGG 0.510652 8:-77481038 None:intergenic
CATAGAGCTGCTCCTCGGTA+AGG 0.512844 8:-77480853 None:intergenic
GCTTATACGAGGGGTTATCA+TGG 0.514631 8:+77480713 MsaT045843.1:CDS
CTTCTTGCGCTTTCTTAGAA+CGG 0.515603 8:-77481571 None:intergenic
AGATGAATATGGTTCATTAA+CGG 0.515829 8:-77476525 None:intergenic
CCGGGATAAATTTGGATGGA+CGG 0.516036 8:+77477702 MsaT045843.1:CDS
ATTCCTTTATGCAGCTGGAA+CGG 0.516374 8:+77481718 MsaT045843.1:CDS
AGCATGGCATGAATCTCATT+GGG 0.516702 8:-77473131 None:intergenic
CAATGACAGATTGCAAAGTC+AGG 0.517201 8:+77476418 MsaT045843.1:intron
ACCATATTCATCTCTGGTAG+TGG 0.517334 8:+77476535 MsaT045843.1:CDS
GATTAAATACTTGTTCTGGA+AGG 0.517408 8:-77476574 None:intergenic
CTTTCTTTGTCTCAAAATTG+CGG 0.517560 8:-77481042 None:intergenic
GAAAGGGCTTCCGTGGGCTC+CGG 0.517612 8:+77481420 MsaT045843.1:CDS
ATCCAACTCACCAAAACCCT+GGG 0.517945 8:+77480669 MsaT045843.1:CDS
ACGAGTTTCAACTTCAAATG+AGG 0.518692 8:+77477321 MsaT045843.1:CDS
GAGAAGTGTAATACGAGTGC+AGG 0.521544 8:+77481541 MsaT045843.1:CDS
CCTGCAGAAATTGTTCAGGA+TGG 0.524642 8:+77477140 MsaT045843.1:CDS
CATGGATTCCACACATTGCA+AGG 0.525083 8:+77472539 MsaT045843.1:CDS
TTGTATGCGTGCTGCTGCAT+CGG 0.525543 8:-77480909 None:intergenic
AGATTATAGGAGGATGAAGT+TGG 0.526731 8:+77481592 MsaT045843.1:CDS
TGCAACTTTGCTGTCAGCTG+GGG 0.527062 8:+77480625 MsaT045843.1:CDS
CGTTGGCGTTTAAAGAGAAA+GGG 0.527109 8:+77481404 MsaT045843.1:CDS
TGCAGAAATTGTTCAGGATG+GGG 0.527605 8:+77477142 MsaT045843.1:CDS
GGACCAAAACTGCATATGCT+GGG 0.528427 8:+77475373 MsaT045843.1:CDS
CCGCTGATCTTGCTTATACG+AGG 0.533890 8:+77480702 MsaT045843.1:CDS
AAGTAGAGATTCACAAATCC+CGG 0.535140 8:-77477194 None:intergenic
CGGTATTAAGCCGGAGCCCA+CGG 0.538463 8:-77481430 None:intergenic
TCAGGACAGCTTTGGAATGT+GGG 0.540299 8:+77476436 MsaT045843.1:CDS
ATTTCTTCTGACAGAATCCA+TGG 0.540689 8:-77475342 None:intergenic
AGTATCACTTAGCAGACTAA+AGG 0.541579 8:+77477397 MsaT045843.1:CDS
GGATGGGGTCTACCGTTGCT+GGG 0.542829 8:+77477157 MsaT045843.1:CDS
AATCATTTGGTCAGTTGGCG+TGG 0.546844 8:+77481364 MsaT045843.1:CDS
CTCTACGAATCTTCCATGTA+CGG 0.546988 8:-77481099 None:intergenic
GAGGAGGAATTCTTTCGGAC+TGG 0.547668 8:+77481491 MsaT045843.1:CDS
TGTCAGAAGAAATAGATTCA+GGG 0.548424 8:+77475352 MsaT045843.1:CDS
ATTGGGCAGCATATTACGGC+AGG 0.549580 8:+77477731 MsaT045843.1:CDS
TTAAAGAGAAAGGGCTTCCG+TGG 0.557276 8:+77481413 MsaT045843.1:CDS
GACCATGTGCATAATATACA+TGG 0.559880 8:-77473831 None:intergenic
ATTCTTCCTCGGTATTAAGC+CGG 0.562168 8:-77481439 None:intergenic
CCATCCTGAACAATTTCTGC+AGG 0.563606 8:-77477140 None:intergenic
ATACTTGTGATTACAAAGCA+TGG 0.565098 8:-77473147 None:intergenic
ATTTCCGGGATAAATTTGGA+TGG 0.565666 8:+77477698 MsaT045843.1:CDS
AACACCTCTGCTGTGCCTAA+CGG 0.566570 8:+77475921 MsaT045843.1:CDS
AGGAATTCTTTCGGACTGGT+AGG 0.566668 8:+77481495 MsaT045843.1:CDS
ATTGCTCACTGCAATTGGCT+GGG 0.569273 8:-77476274 None:intergenic
GAATCAGCAAAGTGATGCAG+AGG 0.570072 8:+77481472 MsaT045843.1:CDS
TCAGCAAAGTGATGCAGAGG+AGG 0.570168 8:+77481475 MsaT045843.1:CDS
GCAGAATCATCAACTGAACA+AGG 0.575288 8:-77476482 None:intergenic
ACACCACTTGTGAGACGGGA+TGG 0.575437 8:-77476374 None:intergenic
GATCCAACTCACCAAAACCC+TGG 0.576628 8:+77480668 MsaT045843.1:CDS
AATCAACACCACTTGTGAGA+CGG 0.580802 8:-77476379 None:intergenic
TATGAAAGAGTCCAGTCACT+AGG 0.581151 8:-77477058 None:intergenic
CTGTGGAAGACACCCACGCA+GGG 0.582346 8:-77476610 None:intergenic
TTCGGAACTTTAGAAAAGAT+GGG 0.582890 8:+77473609 MsaT045843.1:CDS
TTAGCAGACTAAAGGAGGCA+AGG 0.584150 8:+77477405 MsaT045843.1:CDS
CCGTCCATCCAAATTTATCC+CGG 0.585048 8:-77477702 None:intergenic
TAGCAGACTAAAGGAGGCAA+GGG 0.588948 8:+77477406 MsaT045843.1:CDS
TTCCTTAAAGTTACAAACAG+AGG 0.589046 8:+77480949 MsaT045843.1:CDS
ACTTCGCAGATCCTAGTGAC+TGG 0.589671 8:+77477047 MsaT045843.1:intron
TTGAGAAAGCAGTGTTGCGT+TGG 0.592228 8:+77481387 MsaT045843.1:CDS
ACATTAGATTGGACTTTGGG+AGG 0.592424 8:-77477094 None:intergenic
AATCACCATTTACCTCCGTT+AGG 0.593445 8:-77475936 None:intergenic
AAGCGCAAGAAGATTATAGG+AGG 0.598107 8:+77481582 MsaT045843.1:CDS
GTGTTTCACGGTAATGAACA+AGG 0.598368 8:-77474036 None:intergenic
ATCACTTAGCAGACTAAAGG+AGG 0.601189 8:+77477400 MsaT045843.1:CDS
CCATGGTGCAGATGGGTCAG+AGG 0.602933 8:-77475325 None:intergenic
GACCAAAACTGCATATGCTG+GGG 0.606069 8:+77475374 MsaT045843.1:CDS
GATACTCAAAAGTAACAACT+TGG 0.606670 8:-77477244 None:intergenic
ACAGAATCCATGGTGCAGAT+GGG 0.607338 8:-77475332 None:intergenic
TTTCAATGCAATTCCAAACA+GGG 0.609206 8:-77477525 None:intergenic
TCCAATCTAATGTGTGTATG+TGG 0.609379 8:+77477104 MsaT045843.1:CDS
TTCACAAATCCCGGAGAATG+CGG 0.610070 8:-77477185 None:intergenic
GCTGATCTTGCTTATACGAG+GGG 0.612221 8:+77480704 MsaT045843.1:CDS
GGCAAAGCCAAACTTGGTCA+CGG 0.614024 8:+77480646 MsaT045843.1:CDS
CCTCTGACCCATCTGCACCA+TGG 0.617433 8:+77475325 MsaT045843.1:CDS
TAAAGTTCCGAAGCATCTTG+CGG 0.620816 8:-77473598 None:intergenic
AGCTTAGCTGGAAATATCAG+TGG 0.624080 8:+77480788 MsaT045843.1:CDS
TCATTACCGTGAAACACAAG+AGG 0.627907 8:+77474042 MsaT045843.1:CDS
GAAGCTTACCTTGCAATGTG+TGG 0.628537 8:-77472547 None:intergenic
AGAATCATCAACTGAACAAG+GGG 0.629791 8:-77476480 None:intergenic
GCTCTATGTGAAAGATACAC+TGG 0.629798 8:+77480868 MsaT045843.1:CDS
AAGTTGACATGAAAATGAAG+AGG 0.630398 8:+77481764 MsaT045843.1:CDS
TGGAGGAAGCAAGAACGAGA+TGG 0.631278 8:+77473102 MsaT045843.1:CDS
TTACTGATGTGTCCCCTGCG+TGG 0.631639 8:+77476597 MsaT045843.1:CDS
GTCTTCCACAGAGAAATCAA+AGG 0.632840 8:+77476622 MsaT045843.1:CDS
TAAAGAGAAAGGGCTTCCGT+GGG 0.635069 8:+77481414 MsaT045843.1:CDS
ACCTCCGTTAGGCACAGCAG+AGG 0.636051 8:-77475925 None:intergenic
CGATGTTGCGAGCATTATGG+AGG 0.637890 8:+77473085 MsaT045843.1:CDS
AATGCGGTGGTACCCAGCAA+CGG 0.638258 8:-77477169 None:intergenic
ATCCATGTATATTATGCACA+TGG 0.639136 8:+77473829 MsaT045843.1:CDS
CAGAATCATCAACTGAACAA+GGG 0.639781 8:-77476481 None:intergenic
GAACCATCCCGTCTCACAAG+TGG 0.643006 8:+77476371 MsaT045843.1:CDS
GTTGGCCCTTAATCAAGCAA+AGG 0.646848 8:+77481610 MsaT045843.1:CDS
TGGCTCACTGGAAACTAGCA+CGG 0.646998 8:+77480808 MsaT045843.1:CDS
TAAGATCGTTTGCTACGACT+AGG 0.647975 8:-77475899 None:intergenic
TTTCACATAGAGCTGCTCCT+CGG 0.648879 8:-77480858 None:intergenic
TCTGTGGAAGACACCCACGC+AGG 0.652477 8:-77476611 None:intergenic
ATGATGGCAAATAACTTACC+GGG 0.653975 8:+77472494 MsaT045843.1:CDS
GGAAACCTTTGATTTCTCTG+TGG 0.654481 8:-77476627 None:intergenic
TTCATTAACGGATGAAACTG+AGG 0.656620 8:-77476513 None:intergenic
ACTACTGAATATGATGCGCA+AGG 0.659271 8:+77477595 MsaT045843.1:CDS
TCTGATCCAACAAGTTGACC+CGG 0.667535 8:-77472512 None:intergenic
AAGGATATCGAAATGAGCAG+AGG 0.668623 8:+77476349 MsaT045843.1:CDS
ACCTCTGCTGTGCCTAACGG+AGG 0.674338 8:+77475924 MsaT045843.1:CDS
CAGTCAAAAGCCATGAACTG+AGG 0.675587 8:+77475844 MsaT045843.1:CDS
ACAAATCCCGGAGAATGCGG+TGG 0.677994 8:-77477182 None:intergenic
ACCAAAACTGCATATGCTGG+GGG 0.678637 8:+77475375 MsaT045843.1:CDS
TGGGCTCCGGCTTAATACCG+AGG 0.693204 8:+77481433 MsaT045843.1:CDS
TGTGGAAGACACCCACGCAG+GGG 0.697131 8:-77476609 None:intergenic
TATGGAAGAGAAGAACAACT+GGG 0.717360 8:+77477298 MsaT045843.1:CDS
CCATAATGCTCGCAACATCG+AGG 0.721796 8:-77473082 None:intergenic
TCCAACTCACCAAAACCCTG+GGG 0.739177 8:+77480670 MsaT045843.1:CDS
CCTCGTATAAGCAAGATCAG+CGG 0.742504 8:-77480702 None:intergenic
CCAACTCACCAAAACCCTGG+GGG 0.742962 8:+77480671 MsaT045843.1:CDS
AAATTCCGAGAACCTTACCG+AGG 0.753280 8:+77480841 MsaT045843.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 77472491 77481789 77472491 ID=MsaG045843
Chr8 mRNA 77472491 77481789 77472491 ID=MsaT045843.1;Parent=MsaG045843
Chr8 exon 77472491 77472560 77472491 ID=MsaT045843.1.exon1;Parent=MsaT045843.1
Chr8 CDS 77472491 77472560 77472491 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77473081 77473206 77473081 ID=MsaT045843.1.exon2;Parent=MsaT045843.1
Chr8 CDS 77473081 77473206 77473081 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77473576 77473691 77473576 ID=MsaT045843.1.exon3;Parent=MsaT045843.1
Chr8 CDS 77473576 77473691 77473576 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77473811 77473899 77473811 ID=MsaT045843.1.exon4;Parent=MsaT045843.1
Chr8 CDS 77473811 77473899 77473811 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77474018 77474063 77474018 ID=MsaT045843.1.exon5;Parent=MsaT045843.1
Chr8 CDS 77474018 77474063 77474018 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77475279 77475396 77475279 ID=MsaT045843.1.exon6;Parent=MsaT045843.1
Chr8 CDS 77475279 77475396 77475279 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77475826 77475945 77475826 ID=MsaT045843.1.exon7;Parent=MsaT045843.1
Chr8 CDS 77475826 77475945 77475826 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77476035 77476093 77476035 ID=MsaT045843.1.exon8;Parent=MsaT045843.1
Chr8 CDS 77476035 77476093 77476035 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77476228 77476394 77476228 ID=MsaT045843.1.exon9;Parent=MsaT045843.1
Chr8 CDS 77476228 77476394 77476228 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77476427 77476643 77476427 ID=MsaT045843.1.exon10;Parent=MsaT045843.1
Chr8 CDS 77476427 77476643 77476427 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77477056 77477480 77477056 ID=MsaT045843.1.exon11;Parent=MsaT045843.1
Chr8 CDS 77477056 77477480 77477056 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77477522 77477752 77477522 ID=MsaT045843.1.exon12;Parent=MsaT045843.1
Chr8 CDS 77477522 77477752 77477522 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77480613 77481159 77480613 ID=MsaT045843.1.exon13;Parent=MsaT045843.1
Chr8 CDS 77480613 77481159 77480613 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77481323 77481631 77481323 ID=MsaT045843.1.exon14;Parent=MsaT045843.1
Chr8 CDS 77481323 77481631 77481323 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Chr8 exon 77481715 77481789 77481715 ID=MsaT045843.1.exon15;Parent=MsaT045843.1
Chr8 CDS 77481715 77481789 77481715 ID=cds.MsaT045843.1;Parent=MsaT045843.1
Gene Sequence

>MsaT045843.1

ATGATGATGGCAAATAACTTACCGGGTCAACTTGTTGGATCAGAGATACATGGATTCCACACATTGCAAGACCTCGATGTTGCGAGCATTATGGAGGAAGCAAGAACGAGATGGCTTCGTCCCAATGAGATTCATGCCATGCTTTGTAATCACAAGTATTTTACCATCAATGTCAAGCCAATGAATTTGCCTAAAAGTGGTACTATTGTATTGTTTGACCGCAAGATGCTTCGGAACTTTAGAAAAGATGGGCACAACTGGAAAAAGAAAAAGGATGGAAAAACTGTTAAAGAAGCTCATGAGCACTTAAAAGTTGGTAATGAAGAAAGAATCCATGTATATTATGCACATGGTCTAGATAACCCAAATTTTGTTCGTAGATGTTATTGGCTGCTTGACAAGAGCCTGGAACACATCGTCCTTGTTCATTACCGTGAAACACAAGAGTTTCAGGGTTCTCCAATCACTCCCGTAAATTCAAACTCTACTACAGCCTCTGACCCATCTGCACCATGGATTCTGTCAGAAGAAATAGATTCAGGGACCAAAACTGCATATGCTGGGGAGATTAATGATAATATAACAGTCAAAAGCCATGAACTGAGGCTTCACGAGCTTAATACACTTGAATGGGATGACCTAGTCGTAGCAAACGATCTTAACACCTCTGCTGTGCCTAACGGAGGAAAAGTTCCCTATTTTGATCAACAAAATCAAATTTTACTGAATGACAGTTTTAGCAATGTAGCCAACAATGCATCTGCAGAAATTTGTACGTTCAATAATTTAACCCAGCCAATTGCAGTGAGCAATAGTATTCCATACAATTTTTCAGAAAGTGTTACTCTTCAGACAATTAGCAATCAAGGATATCGAAATGAGCAGAGGAACCATCCCGTCTCACAAGTGGTATTGCAAAGTCAGGACAGCTTTGGAATGTGGGTGAACCAAATCATGTCTGATTCCCCTTGTTCAGTTGATGATTCTGCTCTTGGATCCTCAGTTTCATCCGTTAATGAACCATATTCATCTCTGGTAGTGGATAATCAGCAACTTTCCCTTCCAGAACAAGTATTTAATCTTACTGATGTGTCCCCTGCGTGGGTGTCTTCCACAGAGAAATCAAAGATCCTAGTGACTGGACTCTTTCATAAAGACTATCTGCACCTCCCAAAGTCCAATCTAATGTGTGTATGTGGTGATGCAAGTGTTCCTGCAGAAATTGTTCAGGATGGGGTCTACCGTTGCTGGGTACCACCGCATTCTCCGGGATTTGTGAATCTCTACTTGAGCTTTGATGGCCATAACCCTATCAGCCAAGTTGTTACTTTTGAGTATCGTACTCCTGTTTTGCATGATCCAGTAGCTTCTATGGAAGAGAAGAACAACTGGGACGAGTTTCAACTTCAAATGAGGCTTTCTTATTTGCTCTTTGCTAAACAACAGAGTCTTGATGTCATCTCTAGTAAAGTATCACTTAGCAGACTAAAGGAGGCAAGGGAATTCTCCTTAAAGACATCATTTATTTCCAATACTTGGCAATACTTAATGAAATGCCCTGTTTGGAATTGCATTGAAAACAGATTAAAGGAATGGCTTTCAGAAAAAATTGTTTTAGGATGCAAGACTACTGAATATGATGCGCAAGGACAAAGTGTCATCCATTTGTGTGCAATTTTGGAATATACATGGGCAATTACTTTATTTTCATGGTCTGGCTTATCACTAGATTTCCGGGATAAATTTGGATGGACGGCTCTTCATTGGGCAGCATATTACGGCAGGGAGAAAATGGTTGCAACTTTGCTGTCAGCTGGGGCAAAGCCAAACTTGGTCACGGATCCAACTCACCAAAACCCTGGGGGATGTACTGCCGCTGATCTTGCTTATACGAGGGGTTATCATGGCTTAGCAGCTTATCTTTCGGAAAAGTCTCTAGTTGAGCAGTTCAATGACATGAGCTTAGCTGGAAATATCAGTGGCTCACTGGAAACTAGCACGGATGATCCAGTAAATTCCGAGAACCTTACCGAGGAGCAGCTCTATGTGAAAGATACACTGGCAGCTTACCGGACAGCTGCCGATGCAGCAGCACGCATACAAGAAGCATATAGGCAGCATTCCTTAAAGTTACAAACAGAGGCGATTGAGTTTTCCAGCCCAGAAGCTGAAGCACGCAAAATAGTTGCAGCAATGAAGATTCAGCATGCCTTCCGCAATTTTGAGACAAAGAAAGTGATGGTTGCTGCTGCTCGAATACAACATAGATTCCGTACATGGAAGATTCGTAGAGATTTTCTCAACATGCGCCATCAAGCTATCAAGATTCAAGCTGCTTTCAGGTGTTTTCAACAACGGAAACAATATCGTAAAATCATTTGGTCAGTTGGCGTGGTTGAGAAAGCAGTGTTGCGTTGGCGTTTAAAGAGAAAGGGCTTCCGTGGGCTCCGGCTTAATACCGAGGAAGAATCGGGAGATCAGAATCAGCAAAGTGATGCAGAGGAGGAATTCTTTCGGACTGGTAGGAAACAAGCCGAAGAACGTGTCGAGAGAAGTGTAATACGAGTGCAGGCTATGTTCCGTTCTAAGAAAGCGCAAGAAGATTATAGGAGGATGAAGTTGGCCCTTAATCAAGCAAAGAATATTCCTTTATGCAGCTGGAACGGGAATATGAAAATGCTTAGTAATGAAGTTGACATGAAAATGAAGAGGTAG
Protein sequence

>MsaT045843.1

MMMANNLPGQLVGSEIHGFHTLQDLDVASIMEEARTRWLRPNEIHAMLCNHKYFTINVKPMNLPKSGTIVLFDRKMLRNFRKDGHNWKKKKDGKTVKEAHEHLKVGNEERIHVYYAHGLDNPNFVRRCYWLLDKSLEHIVLVHYRETQEFQGSPITPVNSNSTTASDPSAPWILSEEIDSGTKTAYAGEINDNITVKSHELRLHELNTLEWDDLVVANDLNTSAVPNGGKVPYFDQQNQILLNDSFSNVANNASAEICTFNNLTQPIAVSNSIPYNFSESVTLQTISNQGYRNEQRNHPVSQVVLQSQDSFGMWVNQIMSDSPCSVDDSALGSSVSSVNEPYSSLVVDNQQLSLPEQVFNLTDVSPAWVSSTEKSKILVTGLFHKDYLHLPKSNLMCVCGDASVPAEIVQDGVYRCWVPPHSPGFVNLYLSFDGHNPISQVVTFEYRTPVLHDPVASMEEKNNWDEFQLQMRLSYLLFAKQQSLDVISSKVSLSRLKEAREFSLKTSFISNTWQYLMKCPVWNCIENRLKEWLSEKIVLGCKTTEYDAQGQSVIHLCAILEYTWAITLFSWSGLSLDFRDKFGWTALHWAAYYGREKMVATLLSAGAKPNLVTDPTHQNPGGCTAADLAYTRGYHGLAAYLSEKSLVEQFNDMSLAGNISGSLETSTDDPVNSENLTEEQLYVKDTLAAYRTAADAAARIQEAYRQHSLKLQTEAIEFSSPEAEARKIVAAMKIQHAFRNFETKKVMVAAARIQHRFRTWKIRRDFLNMRHQAIKIQAAFRCFQQRKQYRKIIWSVGVVEKAVLRWRLKRKGFRGLRLNTEEESGDQNQQSDAEEEFFRTGRKQAEERVERSVIRVQAMFRSKKAQEDYRRMKLALNQAKNIPLCSWNGNMKMLSNEVDMKMKR*