AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045856


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045856 MtrunA17_Chr4g0068331 97.297 407 11 0 1 407 1 407 0.0 823
MsaG045856 MtrunA17_Chr3g0105631 37.037 405 219 15 13 406 8 387 2.22e-72 231
MsaG045856 MtrunA17_Chr4g0001411 34.390 410 239 11 8 407 20 409 2.62e-63 209
MsaG045856 MtrunA17_Chr4g0053961 33.571 420 241 12 11 407 7 411 3.01e-62 206
MsaG045856 MtrunA17_Chr5g0401351 33.976 415 242 12 7 406 5 402 2.95e-60 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045856 AT4G37580.1 35.817 416 232 14 12 407 3 403 9.97e-69 223
MsaG045856 AT2G23060.1 33.570 423 241 13 11 407 5 413 1.23e-65 215
MsaG045856 AT4G37580.2 33.994 353 204 10 74 407 4 346 7.75e-54 182
MsaG045856 AT2G30090.1 34.390 410 214 20 13 406 15 385 1.74e-53 182
MsaG045856 AT5G67430.1 33.007 409 230 13 11 406 8 385 3.37e-52 179
MsaG045856 AT5G67430.2 31.861 317 196 8 91 406 23 320 3.87e-38 140
MsaG045856 AT2G23060.2 30.208 288 188 7 124 407 1 279 9.23e-36 133

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTGTGCAAACTCCTTC+TGG 0.261524 8:+77609611 MsaT045856.1:CDS
GAGCTTGTTGGAGTTGTTAA+AGG 0.270934 8:+77609581 MsaT045856.1:CDS
GATTAATGGGACTACTAAAA+AGG 0.272190 8:+77609294 MsaT045856.1:CDS
AGGGTTGTTATAACTGAGTT+AGG 0.299823 8:+77610640 MsaT045856.1:CDS
GTGACCCTTTATCTAGGATT+AGG 0.304028 8:+77609350 MsaT045856.1:CDS
AGTTGCATTTGTTGGTGAAT+AGG 0.308058 8:-77610057 None:intergenic
TTTCAAGATTCTTGTGTAGA+AGG 0.309351 8:-77610194 None:intergenic
AGAAACTGTACTGAGATTAA+TGG 0.318075 8:+77609280 MsaT045856.1:CDS
TAGAACCTAATCCTAGATAA+AGG 0.318214 8:-77609355 None:intergenic
ATCTAATGGATACAACTCTT+TGG 0.333407 8:-77610218 None:intergenic
GTTGGTGAATAGGTTATTAG+AGG 0.345184 8:-77610047 None:intergenic
TTCTTAGAAATGTGGTTTGT+TGG 0.348075 8:-77610130 None:intergenic
ATGTGTAACAACAGTACATT+TGG 0.356097 8:+77610511 MsaT045856.1:CDS
GAAACTGTACTGAGATTAAT+GGG 0.378728 8:+77609281 MsaT045856.1:CDS
AGAATAATGTCCATATCTAA+TGG 0.380464 8:-77610232 None:intergenic
GCGGGCTAATGGAATCAATA+TGG 0.381668 8:+77610578 MsaT045856.1:CDS
AGAGAATCTTGGCGGGCTAA+TGG 0.382594 8:+77610567 MsaT045856.1:CDS
TCTTAGAAATGTGGTTTGTT+GGG 0.383397 8:-77610129 None:intergenic
GTTATAACTGAGTTAGGGTT+TGG 0.401121 8:+77610646 MsaT045856.1:CDS
TTCAAGATGGGTTGTATACT+AGG 0.416953 8:+77609641 MsaT045856.1:CDS
TGATGATGAAAATGATGAAT+TGG 0.425086 8:+77610756 MsaT045856.1:CDS
TGGAGGTTTACATCAAGATT+AGG 0.428439 8:+77610598 MsaT045856.1:CDS
CATGATAATTACCTGTGTAT+AGG 0.429059 8:-77609677 None:intergenic
TTCTGGATCACTCTTCAAGA+TGG 0.429973 8:+77609628 MsaT045856.1:CDS
ATTGAAATAGTTGCATTTGT+TGG 0.433692 8:-77610065 None:intergenic
GTTGAAAACAAGGAGCTTGT+TGG 0.434978 8:+77609569 MsaT045856.1:CDS
AGAGTTGTATCCATTAGATA+TGG 0.437070 8:+77610222 MsaT045856.1:CDS
AAATTTACTGTGCAGGAGAA+AGG 0.442722 8:+77609931 MsaT045856.1:intron
GTAATGGTGACCCTTTATCT+AGG 0.452354 8:+77609344 MsaT045856.1:CDS
GGAATGTGTTTGTTGATCCT+AGG 0.455878 8:+77610803 MsaT045856.1:CDS
CAAAATTATTTCAACCACTT+AGG 0.457975 8:+77610422 MsaT045856.1:CDS
GGGTTGTTATAACTGAGTTA+GGG 0.463044 8:+77610641 MsaT045856.1:CDS
CTATCCCCTTCGTGTTATGT+TGG 0.463391 8:+77609375 MsaT045856.1:CDS
TAGCATCCACTCTTCAATTG+AGG 0.471060 8:-77609975 None:intergenic
GTTAGTTATTACAAAGATGA+AGG 0.474099 8:+77610283 MsaT045856.1:CDS
CATGGAGAGGGAGAGAATCT+TGG 0.478715 8:+77610556 MsaT045856.1:CDS
TGTGTATAGGAGAGACTCTA+AGG 0.479289 8:-77609664 None:intergenic
AATAACTAACCCAAGTTCCT+AGG 0.480250 8:-77610270 None:intergenic
TCTGGATCACTCTTCAAGAT+GGG 0.484366 8:+77609629 MsaT045856.1:CDS
GATAGAGACGTTAAAGTGGT+GGG 0.487062 8:+77609247 MsaT045856.1:CDS
GAATGTGTTTGTTGATCCTA+GGG 0.487462 8:+77610804 MsaT045856.1:CDS
AGAACCTAATCCTAGATAAA+GGG 0.491131 8:-77609354 None:intergenic
CACGACATAGAATCAATTTG+AGG 0.498622 8:-77610691 None:intergenic
AATTTACTGTGCAGGAGAAA+GGG 0.506218 8:+77609932 MsaT045856.1:intron
TGTGTAACAACAGTACATTT+GGG 0.506653 8:+77610512 MsaT045856.1:CDS
AACAAACCACATTTCTAAGA+AGG 0.512894 8:+77610132 MsaT045856.1:CDS
TTGGGAAACTAGTTGGTGGA+TGG 0.520506 8:-77610111 None:intergenic
TTCACCAACATGATGAGTAA+TGG 0.521767 8:+77609328 MsaT045856.1:CDS
CTTTATGGACTTCATGGAGA+GGG 0.530822 8:+77610544 MsaT045856.1:CDS
TGGTTTGTTGGGAAACTAGT+TGG 0.533846 8:-77610118 None:intergenic
AGCTGAGATGGTTGAAAACA+AGG 0.541254 8:+77609559 MsaT045856.1:CDS
AAAGTACAACAAGCTCATCA+TGG 0.542735 8:+77610341 MsaT045856.1:CDS
AAAACTCAGCCTAGGAACTT+GGG 0.550630 8:+77610261 MsaT045856.1:CDS
TTTGTTGGGAAACTAGTTGG+TGG 0.550774 8:-77610115 None:intergenic
TGACATCCTTCTTAGAAATG+TGG 0.553319 8:-77610138 None:intergenic
TGATGAAAATGATGAATTGG+TGG 0.563601 8:+77610759 MsaT045856.1:CDS
TGAGGAACATGGTTTACAAG+TGG 0.564375 8:-77610673 None:intergenic
ATAGAGACGTTAAAGTGGTG+GGG 0.567023 8:+77609248 MsaT045856.1:CDS
GGAGAGGGAGAGAATCTTGG+CGG 0.572469 8:+77610559 MsaT045856.1:CDS
GAAGAGTGGATGCTAAGAAA+TGG 0.583171 8:+77609983 MsaT045856.1:CDS
TGAAGATAGAGACGTTAAAG+TGG 0.583839 8:+77609243 MsaT045856.1:CDS
TAGAATCAATTTGAGGAACA+TGG 0.588050 8:-77610684 None:intergenic
AGATAGAGACGTTAAAGTGG+TGG 0.592949 8:+77609246 MsaT045856.1:CDS
CTGTGCAGGAGAAAGGGAGT+TGG 0.597746 8:+77609938 MsaT045856.1:intron
GTCGCATGAAGAAACCTAAG+TGG 0.602408 8:-77610436 None:intergenic
TGCGACATTAAATCATGCAA+AGG 0.605817 8:+77610453 MsaT045856.1:CDS
TGGCCTGCAGGTAGCTGAGA+TGG 0.614618 8:+77609547 MsaT045856.1:intron
GAGAGGGAGAGAATCTTGGC+GGG 0.615284 8:+77610560 MsaT045856.1:CDS
GAGGTGATGAAGAGACAACT+CGG 0.616820 8:+77610781 MsaT045856.1:CDS
ACACACCAACATAACACGAA+GGG 0.618929 8:-77609380 None:intergenic
GTCACCATTACTCATCATGT+TGG 0.630070 8:-77609332 None:intergenic
TTGTAACCTCAATTGAAGAG+TGG 0.632770 8:+77609969 MsaT045856.1:CDS
CACATACAGTATAGAGAGCG+AGG 0.638269 8:+77609201 MsaT045856.1:CDS
TGTCGTGTATTGATGATATG+TGG 0.645905 8:+77610707 MsaT045856.1:CDS
AGGTGATGAAGAGACAACTC+GGG 0.647405 8:+77610782 MsaT045856.1:CDS
TCTTTATGGACTTCATGGAG+AGG 0.647924 8:+77610543 MsaT045856.1:CDS
TGAAAATGATGAATTGGTGG+AGG 0.648658 8:+77610762 MsaT045856.1:CDS
TTAGAGTCTCTCCTATACAC+AGG 0.650718 8:+77609666 MsaT045856.1:CDS
GAGACGTTAAAGTGGTGGGG+AGG 0.666370 8:+77609251 MsaT045856.1:CDS
CACACCAACATAACACGAAG+GGG 0.668902 8:-77609379 None:intergenic
TACACACCAACATAACACGA+AGG 0.672948 8:-77609381 None:intergenic
GGCTAATGGAATCAATATGG+AGG 0.674786 8:+77610581 MsaT045856.1:CDS
ATGGAGATATATGATGAGTG+AGG 0.681980 8:-77610092 None:intergenic
GAGTCAAACATTCTCAAACA+AGG 0.685595 8:-77610487 None:intergenic
ATCTTGAAGAGTGATCCAGA+AGG 0.751962 8:-77609626 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 77609193 77610834 77609193 ID=MsaG045856
Chr8 mRNA 77609193 77610834 77609193 ID=MsaT045856.1;Parent=MsaG045856
Chr8 exon 77609193 77609396 77609193 ID=MsaT045856.1.exon1;Parent=MsaT045856.1
Chr8 CDS 77609193 77609396 77609193 ID=cds.MsaT045856.1;Parent=MsaT045856.1
Chr8 exon 77609557 77609687 77609557 ID=MsaT045856.1.exon2;Parent=MsaT045856.1
Chr8 CDS 77609557 77609687 77609557 ID=cds.MsaT045856.1;Parent=MsaT045856.1
Chr8 exon 77609946 77610834 77609946 ID=MsaT045856.1.exon3;Parent=MsaT045856.1
Chr8 CDS 77609946 77610834 77609946 ID=cds.MsaT045856.1;Parent=MsaT045856.1
Gene Sequence

>MsaT045856.1

ATGGCTGACACATACAGTATAGAGAGCGAGGTTGTTATAAGAGAGTTTGATGAAGATAGAGACGTTAAAGTGGTGGGGAGGCTTGAAAGAAACTGTACTGAGATTAATGGGACTACTAAAAAGGGTTTATCTATTTTCACCAACATGATGAGTAATGGTGACCCTTTATCTAGGATTAGGTTCTATCCCCTTCGTGTTATGTTGGTAGCTGAGATGGTTGAAAACAAGGAGCTTGTTGGAGTTGTTAAAGGATGCATTAAAAGTGTGCAAACTCCTTCTGGATCACTCTTCAAGATGGGTTGTATACTAGGCCTTAGAGTCTCTCCTATACACAGGAGAAAGGGAGTTGGACTAAAACTTGTAACCTCAATTGAAGAGTGGATGCTAAGAAATGGTGCTGATTATGCCTTCCTAGCCACAGAAAAGAATAACAATGCCTCTAATAACCTATTCACCAACAAATGCAACTATTTCAATCTCACCTCACTCATCATATATCTCCATCCACCAACTAGTTTCCCAACAAACCACATTTCTAAGAAGGATGTCAAAATTGACAAGATAAATATAGATCAAGCAATTTCCTTCTACACAAGAATCTTGAAAACCAAAGAGTTGTATCCATTAGATATGGACATTATTCTCAAAGAAAAACTCAGCCTAGGAACTTGGGTTAGTTATTACAAAGATGAAGGCTTCAAACTCAACATTGAAGATATTATTACCCATAAAAGTACAACAAGCTCATCATGGATCATTTTCAGTTTATGGAATACTTGTGAAGCTTGTGACAATAACCTTCAAGTTAAAACAAAATTATTTCAACCACTTAGGTTTCTTCATGCGACATTAAATCATGCAAAGGACAAAATATGTCCTTGTTTGAGAATGTTTGACTCAATGTGTAACAACAGTACATTTGGGTTTCTTTTTCTTTATGGACTTCATGGAGAGGGAGAGAATCTTGGCGGGCTAATGGAATCAATATGGAGGTTTACATCAAGATTAGGAGAAAAATTAAAGGAGTGTAGGGTTGTTATAACTGAGTTAGGGTTTGGTGATCCACTTGTAAACCATGTTCCTCAAATTGATTCTATGTCGTGTATTGATGATATGTGGTACACAAAAAGACTTGGAAATCACAGTGATGATGAAAATGATGAATTGGTGGAGGTGATGAAGAGACAACTCGGGAATGTGTTTGTTGATCCTAGGGATTTTTAG

Protein sequence

>MsaT045856.1

MADTYSIESEVVIREFDEDRDVKVVGRLERNCTEINGTTKKGLSIFTNMMSNGDPLSRIRFYPLRVMLVAEMVENKELVGVVKGCIKSVQTPSGSLFKMGCILGLRVSPIHRRKGVGLKLVTSIEEWMLRNGADYAFLATEKNNNASNNLFTNKCNYFNLTSLIIYLHPPTSFPTNHISKKDVKIDKINIDQAISFYTRILKTKELYPLDMDIILKEKLSLGTWVSYYKDEGFKLNIEDIITHKSTTSSSWIIFSLWNTCEACDNNLQVKTKLFQPLRFLHATLNHAKDKICPCLRMFDSMCNNSTFGFLFLYGLHGEGENLGGLMESIWRFTSRLGEKLKECRVVITELGFGDPLVNHVPQIDSMSCIDDMWYTKRLGNHSDDENDELVEVMKRQLGNVFVDPRDF*