AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046012

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046012 MtrunA17_Chr4g0070271 70.755 106 30 1 1 106 67 171 1.34e-44 144
MsaG046012 MtrunA17_Chr4g0070241 92.188 64 5 0 1 64 1 64 5.88e-37 120
MsaG046012 MtrunA17_Chr2g0301131 38.608 158 44 6 1 106 72 228 3.04e-22 87.8
MsaG046012 MtrunA17_Chr5g0399851 36.190 105 44 3 25 106 11 115 4.32e-14 64.3
MsaG046012 MtrunA17_Chr5g0399881 37.692 130 53 5 7 108 5 134 1.26e-13 63.5
MsaG046012 MtrunA17_Chr5g0399861 37.234 94 42 2 30 106 39 132 8.46e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046012 AT1G19180.2 32.609 138 59 3 2 105 17 154 4.45e-11 57.4
MsaG046012 AT1G19180.1 36.190 105 41 2 30 108 119 223 8.39e-11 57.4
MsaG046012 AT1G19180.3 36.190 105 41 2 30 108 119 223 8.39e-11 57.4

Find 21 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTCACTTCTTCGATTCA+TGG 0.237608 8:-79531946 MsaT046012.1:CDS
AATGTCAACTGTGCAGTTTC+CGG 0.323565 8:+79532240 None:intergenic
TCTAGTAACACAAGTGGCTT+TGG 0.360249 8:+79531340 None:intergenic
CAACCACCTCCTACACTAAC+TGG 0.374106 8:-79532102 MsaT046012.1:intron
TTATTTCTTTAAAGAAGATA+TGG 0.376656 8:-79531982 MsaT046012.1:intron
TAATGGAGAAGTTATTGTAT+TGG 0.400656 8:-79532211 MsaT046012.1:CDS
TAACTCAATGGTTAACAAAC+CGG 0.425259 8:-79532259 MsaT046012.1:CDS
GGTGGTTGAAGACTCATATC+AGG 0.439597 8:+79532117 None:intergenic
AGCTCCTCAACTTTGTCAGC+AGG 0.455494 8:+79532180 None:intergenic
TTACAGAACTAATAACTCAA+TGG 0.476118 8:-79532271 MsaT046012.1:intron
TTCTTCGATTCATGGAGAAA+AGG 0.478429 8:-79531938 MsaT046012.1:CDS
CAATTACCAGTTAGTGTAGG+AGG 0.522487 8:+79532096 None:intergenic
CTGTGGATTCTTTGTCTCCA+AGG 0.531713 8:-79532415 MsaT046012.1:intron
TCATGGAGAAAAGGAAAGCC+AGG 0.536399 8:-79531929 MsaT046012.1:intron
CATTTGCAAGCACTCAAACT+CGG 0.560353 8:-79532152 MsaT046012.1:CDS
CTCATATCAGGAATAACTGA+AGG 0.598557 8:+79532129 None:intergenic
TCAATTTCTAGTAACACAAG+TGG 0.602083 8:+79531334 None:intergenic
GTTTGTGGGAAATAAGTCCA+TGG 0.603000 8:+79532460 None:intergenic
TTACCAGTTAGTGTAGGAGG+TGG 0.608915 8:+79532099 None:intergenic
GAGCAATTACCAGTTAGTGT+AGG 0.610011 8:+79532093 None:intergenic
CTTGGAGACAAAGAATCCAC+AGG 0.648213 8:+79532416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 79531327 79532480 79531327 ID=MsaG046012
Chr8 mRNA 79531327 79532480 79531327 ID=MsaT046012.1;Parent=MsaG046012
Chr8 exon 79531327 79531375 79531327 ID=MsaT046012.1.exon4;Parent=MsaT046012.1
Chr8 CDS 79531327 79531375 79531327 ID=cds.MsaT046012.1;Parent=MsaT046012.1
Chr8 exon 79531930 79531990 79531930 ID=MsaT046012.1.exon3;Parent=MsaT046012.1
Chr8 CDS 79531930 79531990 79531930 ID=cds.MsaT046012.1;Parent=MsaT046012.1
Chr8 exon 79532103 79532287 79532103 ID=MsaT046012.1.exon2;Parent=MsaT046012.1
Chr8 CDS 79532103 79532287 79532103 ID=cds.MsaT046012.1;Parent=MsaT046012.1
Chr8 exon 79532416 79532480 79532416 ID=MsaT046012.1.exon1;Parent=MsaT046012.1
Chr8 CDS 79532416 79532480 79532416 ID=cds.MsaT046012.1;Parent=MsaT046012.1
Gene Sequence

>MsaT046012.1

ATGGACTTATTTCCCACAAACGTGACACCAAAAAACGTTACACCTGTGGATTCTTTGTCTCCAAGAACTAATAACTCAATGGTTAACAAACCGGAAACTGCACAGTTGACATTGTTTTATAATGGAGAAGTTATTGTATTGGATGATTTTCCTGCTGACAAAGTTGAGGAGCTTAAGTCATTTGCAAGCACTCAAACTCGGCCTTCAGTTATTCCTGATATGAGTCTTCAACCACCTCCTACACTAACTGAAGATATGGTAATTGCTAGAAAAGCTTCACTTCTTCGATTCATGGAGAAAAGGAAAGCCAGGCTTGCTGAAGAGCCAAAGCCACTTGTGTTACTAGAAATTGATGCATAA
Protein sequence

>MsaT046012.1

MDLFPTNVTPKNVTPVDSLSPRTNNSMVNKPETAQLTLFYNGEVIVLDDFPADKVEELKSFASTQTRPSVIPDMSLQPPPTLTEDMVIARKASLLRFMEKRKARLAEEPKPLVLLEIDA*