Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG046013 | XP_003609979.1 | 92.683 | 205 | 11 | 2 | 1 | 204 | 1 | 202 | 1.03e-124 | 360 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG046013 | sp|Q9LMA8|TI10A_ARATH | 36.328 | 256 | 106 | 10 | 1 | 203 | 1 | 252 | 1.25e-34 | 126 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG046013 | tr|G7JS78|G7JS78_MEDTR | 92.683 | 205 | 11 | 2 | 1 | 204 | 1 | 202 | 4.93e-125 | 360 |
Gene ID | Type | Classification |
---|---|---|
MsaG046013 | TF | Tify |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000071 | MsaG046013 | 0.811377 | 1.238030e-50 | 2.186502e-48 |
MsaG000118 | MsaG046013 | 0.839876 | 2.250896e-57 | 8.753090e-55 |
MsaG000236 | MsaG046013 | 0.813452 | 4.380601e-51 | 8.145111e-49 |
MsaG000306 | MsaG046013 | 0.827296 | 3.018628e-54 | 8.093472e-52 |
MsaG000523 | MsaG046013 | 0.815401 | 1.631238e-51 | 3.185510e-49 |
MsaG000692 | MsaG046013 | 0.825172 | 9.612385e-54 | 2.429500e-51 |
MsaG001328 | MsaG046013 | 0.835143 | 3.630007e-56 | 1.221124e-53 |
MsaG001660 | MsaG046013 | 0.823862 | 1.949315e-53 | 4.753648e-51 |
MsaG002587 | MsaG046013 | 0.807390 | 8.790253e-50 | 1.409699e-47 |
MsaG002964 | MsaG046013 | 0.810024 | 2.419637e-50 | 4.134428e-48 |
MsaG002966 | MsaG046013 | 0.808277 | 5.707018e-50 | 9.349503e-48 |
MsaG003192 | MsaG046013 | 0.808734 | 4.563068e-50 | 7.558394e-48 |
MsaG003289 | MsaG046013 | 0.809254 | 3.535807e-50 | 5.930155e-48 |
MsaG003515 | MsaG046013 | 0.822190 | 4.763767e-53 | 1.110348e-50 |
MsaG003649 | MsaG046013 | 0.816424 | 9.666898e-52 | 1.937449e-49 |
MsaG004133 | MsaG046013 | 0.804073 | 4.339425e-49 | 6.439099e-47 |
MsaG004441 | MsaG046013 | 0.866100 | 7.318083e-65 | 7.224855e-62 |
MsaG004757 | MsaG046013 | 0.851113 | 2.093858e-60 | 1.179199e-57 |
MsaG004913 | MsaG046013 | 0.810436 | 1.974568e-50 | 3.408132e-48 |
MsaG005223 | MsaG046013 | 0.840936 | 1.192458e-57 | 4.794050e-55 |
MsaG005229 | MsaG046013 | 0.800644 | 2.189117e-48 | 3.003230e-46 |
MsaG005334 | MsaG046013 | 0.804088 | 4.307831e-49 | 6.394496e-47 |
MsaG005346 | MsaG046013 | 0.806804 | 1.168254e-49 | 1.847719e-47 |
MsaG005512 | MsaG046013 | 0.811179 | 1.365730e-50 | 2.400367e-48 |
MsaG005719 | MsaG046013 | 0.878639 | 5.007898e-69 | 8.487845e-66 |
MsaG006593 | MsaG046013 | 0.807962 | 6.652980e-50 | 1.081699e-47 |
MsaG007240 | MsaG046013 | 0.801944 | 1.189372e-48 | 1.680409e-46 |
MsaG007454 | MsaG046013 | 0.813315 | 4.692739e-51 | 8.695355e-49 |
MsaG007816 | MsaG046013 | 0.832473 | 1.676169e-55 | 5.210389e-53 |
MsaG008880 | MsaG046013 | 0.810339 | 2.071702e-50 | 3.567286e-48 |
MsaG009261 | MsaG046013 | 0.828496 | 1.557772e-54 | 4.319814e-52 |
MsaG009551 | MsaG046013 | 0.847283 | 2.407329e-59 | 1.190063e-56 |
MsaG009850 | MsaG046013 | 0.808293 | 5.660906e-50 | 9.277489e-48 |
MsaG009872 | MsaG046013 | 0.833090 | 1.179826e-55 | 3.734416e-53 |
MsaG010345 | MsaG046013 | 0.846193 | 4.764131e-59 | 2.270892e-56 |
MsaG012446 | MsaG046013 | 0.804732 | 3.167492e-49 | 4.772261e-47 |
MsaG012507 | MsaG046013 | 0.832782 | 1.406009e-55 | 4.410219e-53 |
MsaG012633 | MsaG046013 | 0.829872 | 7.250775e-55 | 2.090843e-52 |
MsaG042351 | MsaG046013 | 0.816016 | 1.191889e-51 | 2.364053e-49 |
MsaG043571 | MsaG046013 | 0.805189 | 2.544220e-49 | 3.874214e-47 |
MsaG045060 | MsaG046013 | 0.811933 | 9.382775e-51 | 1.679943e-48 |
MsaG045062 | MsaG046013 | 0.805432 | 2.264372e-49 | 3.467451e-47 |
MsaG045107 | MsaG046013 | 0.861825 | 1.543679e-63 | 1.286748e-60 |
MsaG045108 | MsaG046013 | 0.823369 | 2.539463e-53 | 6.110756e-51 |
MsaG045145 | MsaG046013 | 0.805424 | 2.272725e-49 | 3.479615e-47 |
MsaG045451 | MsaG046013 | 0.808070 | 6.313733e-50 | 1.029199e-47 |
MsaG045506 | MsaG046013 | 0.809608 | 2.971023e-50 | 5.025835e-48 |
MsaG045952 | MsaG046013 | 0.814495 | 2.585076e-51 | 4.933947e-49 |
MsaG046013 | MsaG046113 | 0.836206 | 1.959287e-56 | 6.804550e-54 |
MsaG046013 | MsaG046114 | 0.802128 | 1.090637e-48 | 1.547345e-46 |
MsaG046013 | MsaG046614 | 0.824361 | 1.489939e-53 | 3.683263e-51 |
MsaG046013 | MsaG046928 | 0.816168 | 1.102658e-51 | 2.195605e-49 |
MsaG046013 | MsaG047139 | 0.815125 | 1.877376e-51 | 3.640437e-49 |
MsaG046013 | MsaG002247 | 0.808123 | 6.150576e-50 | 1.003899e-47 |
MsaG046013 | MsaG008516 | 0.829163 | 1.076225e-54 | 3.040830e-52 |
MsaG046013 | MsaG008455 | 0.835883 | 2.364492e-56 | 8.132333e-54 |
MsaG046013 | MsaG012735 | 0.831041 | 3.765929e-55 | 1.122866e-52 |
MsaG046013 | MsaG013871 | 0.803601 | 5.431024e-49 | 7.970859e-47 |
MsaG046013 | MsaG026455 | 0.805116 | 2.634650e-49 | 4.005062e-47 |
MsaG046013 | MsaG029566 | 0.802108 | 1.101195e-48 | 1.561580e-46 |
MsaG046013 | MsaG026065 | 0.826946 | 3.658829e-54 | 9.714170e-52 |
MsaG046013 | MsaG026951 | 0.806078 | 1.658916e-49 | 2.579185e-47 |
MsaG046013 | MsaG033554 | 0.803378 | 6.039557e-49 | 8.818466e-47 |
MsaG046013 | MsaG031296 | 0.800281 | 2.593194e-48 | 3.528396e-46 |
MsaG046013 | MsaG036797 | 0.835897 | 2.345265e-56 | 8.069554e-54 |
MsaG046013 | MsaG034989 | 0.810193 | 2.226321e-50 | 3.819740e-48 |
MsaG046013 | MsaG037655 | 0.842020 | 6.200824e-58 | 2.580315e-55 |
MsaG046013 | MsaG035293 | 0.852021 | 1.161566e-60 | 6.753223e-58 |
MsaG046013 | MsaG038926 | 0.824816 | 1.165683e-53 | 2.917542e-51 |
MsaG046013 | MsaG037052 | 0.842533 | 4.540161e-58 | 1.920643e-55 |
MsaG046013 | MsaG038026 | 0.816290 | 1.035511e-51 | 2.068346e-49 |
MsaG046013 | MsaG039898 | 0.827984 | 2.067318e-54 | 5.650852e-52 |
MsaG046013 | MsaG042416 | 0.809189 | 3.650793e-50 | 6.113456e-48 |
MsaG046013 | MsaG045038 | 0.809287 | 3.478571e-50 | 5.838766e-48 |
MsaG012671 | MsaG046013 | 0.859230 | 9.358835e-63 | 7.066948e-60 |
MsaG015039 | MsaG046013 | 0.800616 | 2.218059e-48 | 3.041014e-46 |
MsaG016406 | MsaG046013 | 0.816840 | 7.810610e-52 | 1.582196e-49 |
MsaG016837 | MsaG046013 | 0.806840 | 1.148189e-49 | 1.817484e-47 |
MsaG016946 | MsaG046013 | 0.812557 | 6.865134e-51 | 1.248298e-48 |
MsaG017083 | MsaG046013 | 0.815841 | 1.303478e-51 | 2.573936e-49 |
MsaG017193 | MsaG046013 | 0.801357 | 1.567776e-48 | 2.185659e-46 |
MsaG017417 | MsaG046013 | 0.834136 | 6.483367e-56 | 2.116205e-53 |
MsaG017773 | MsaG046013 | 0.823243 | 2.715478e-53 | 6.512073e-51 |
MsaG017972 | MsaG046013 | 0.846727 | 3.411946e-59 | 1.655669e-56 |
MsaG018121 | MsaG046013 | 0.804125 | 4.232414e-49 | 6.287774e-47 |
MsaG018259 | MsaG046013 | 0.805723 | 1.968453e-49 | 3.034919e-47 |
MsaG018436 | MsaG046013 | 0.809108 | 3.797947e-50 | 6.347830e-48 |
MsaG018752 | MsaG046013 | 0.812864 | 5.886679e-51 | 1.078564e-48 |
MsaG019273 | MsaG046013 | 0.802946 | 7.411759e-49 | 1.071450e-46 |
MsaG019296 | MsaG046013 | 0.812147 | 8.428955e-51 | 1.517040e-48 |
MsaG019951 | MsaG046013 | 0.868135 | 1.651283e-65 | 1.771208e-62 |
MsaG020131 | MsaG046013 | 0.806653 | 1.256688e-49 | 1.980583e-47 |
MsaG020747 | MsaG046013 | 0.848554 | 1.078440e-59 | 5.563762e-57 |
MsaG020765 | MsaG046013 | 0.840880 | 1.233447e-57 | 4.949966e-55 |
MsaG021105 | MsaG046013 | 0.851602 | 1.525142e-60 | 8.735513e-58 |
MsaG021516 | MsaG046013 | 0.805487 | 2.205386e-49 | 3.381417e-47 |
MsaG021665 | MsaG046013 | 0.815786 | 1.340586e-51 | 2.643482e-49 |
MsaG022294 | MsaG046013 | 0.805456 | 2.238119e-49 | 3.429228e-47 |
MsaG023475 | MsaG046013 | 0.824499 | 1.383138e-53 | 3.432056e-51 |
MsaG023816 | MsaG046013 | 0.839502 | 2.813687e-57 | 1.081338e-54 |
MsaG023857 | MsaG046013 | 0.823158 | 2.842700e-53 | 6.801217e-51 |
MsaG025732 | MsaG046013 | 0.810482 | 1.930141e-50 | 3.335105e-48 |
MsaG026903 | MsaG046013 | 0.812994 | 5.513815e-51 | 1.013535e-48 |
MsaG027743 | MsaG046013 | 0.817331 | 6.062925e-52 | 1.243875e-49 |
MsaG027863 | MsaG046013 | 0.830551 | 4.960654e-55 | 1.458350e-52 |
MsaG028142 | MsaG046013 | 0.814313 | 2.835765e-51 | 5.387746e-49 |
MsaG028871 | MsaG046013 | 0.850878 | 2.437221e-60 | 1.361519e-57 |
MsaG029075 | MsaG046013 | 0.833113 | 1.164455e-55 | 3.688352e-53 |
MsaG029421 | MsaG046013 | 0.811638 | 1.087053e-50 | 1.932157e-48 |
MsaG030115 | MsaG046013 | 0.803921 | 4.665390e-49 | 6.898116e-47 |
MsaG034837 | MsaG046013 | 0.814794 | 2.221267e-51 | 4.271703e-49 |
MsaG036450 | MsaG046013 | 0.837575 | 8.794142e-57 | 3.183530e-54 |
MsaG040593 | MsaG046013 | 0.826348 | 5.073595e-54 | 1.324818e-51 |
MsaG040733 | MsaG046013 | 0.814660 | 2.377791e-51 | 4.557135e-49 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG046013 | MtrunA17_Chr4g0070271 | 92.683 | 205 | 11 | 2 | 1 | 204 | 1 | 202 | 9.49e-129 | 360 |
MsaG046013 | MtrunA17_Chr2g0301131 | 44.828 | 261 | 76 | 11 | 1 | 204 | 1 | 250 | 2.28e-49 | 161 |
MsaG046013 | MtrunA17_Chr4g0070241 | 78.125 | 64 | 14 | 0 | 70 | 133 | 1 | 64 | 1.02e-28 | 102 |
MsaG046013 | MtrunA17_Chr5g0399881 | 36.875 | 160 | 69 | 6 | 76 | 204 | 5 | 163 | 1.38e-21 | 87.0 |
MsaG046013 | MtrunA17_Chr5g0399861 | 32.927 | 164 | 69 | 5 | 76 | 204 | 5 | 162 | 2.84e-21 | 86.3 |
MsaG046013 | MtrunA17_Chr5g0399851 | 36.364 | 132 | 60 | 2 | 94 | 201 | 11 | 142 | 2.94e-20 | 83.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG046013 | AT1G19180.1 | 36.328 | 256 | 106 | 10 | 1 | 203 | 1 | 252 | 1.27e-35 | 126 |
MsaG046013 | AT1G19180.3 | 36.328 | 256 | 106 | 10 | 1 | 203 | 1 | 252 | 1.27e-35 | 126 |
MsaG046013 | AT1G74950.1 | 36.100 | 241 | 75 | 12 | 7 | 180 | 1 | 229 | 5.18e-27 | 103 |
MsaG046013 | AT1G74950.2 | 36.052 | 233 | 70 | 12 | 7 | 172 | 1 | 221 | 1.23e-23 | 94.4 |
MsaG046013 | AT1G19180.2 | 39.098 | 133 | 52 | 5 | 99 | 203 | 55 | 186 | 1.26e-18 | 80.1 |
MsaG046013 | AT1G17380.1 | 29.353 | 201 | 108 | 5 | 13 | 180 | 7 | 206 | 8.79e-14 | 68.6 |
Find 43 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TCGACTTCATCGGACATTTC+TGG | 0.184990 | 8:-79543137 | MsaT046013.1:CDS |
ATTGTCAATTGTGCAGTTTC+TGG | 0.207124 | 8:+79542514 | None:intergenic |
CCTTCTTCTCCTTGAGGTAT+TGG | 0.218986 | 8:+79543045 | None:intergenic |
GGTTGTTGAGGGATCATATT+AGG | 0.246891 | 8:+79542391 | None:intergenic |
CAACAACCTCCCTCACTAAT+TGG | 0.267401 | 8:-79542376 | MsaT046013.1:intron |
AGCTTCACTTCGTCGATTCA+TGG | 0.304214 | 8:-79542195 | MsaT046013.1:CDS |
TCAGGAAAGGTTCTTTGATA+TGG | 0.341074 | 8:+79541747 | None:intergenic |
TTTATTTACCATTTGTTTCA+GGG | 0.363709 | 8:+79542985 | None:intergenic |
CGACTTTATCAGTTTGTTGC+CGG | 0.376712 | 8:+79543109 | None:intergenic |
AGCTCCTCAACTTTCTCAGC+AGG | 0.410410 | 8:+79542454 | None:intergenic |
TCCGCTCCATGGCTTGTGTT+AGG | 0.414480 | 8:-79541699 | MsaT046013.1:CDS |
TCATGGAGAAGAGGAAAGAT+AGG | 0.451183 | 8:-79542178 | MsaT046013.1:intron |
CCAATACCTCAAGGAGAAGA+AGG | 0.480824 | 8:-79543045 | MsaT046013.1:CDS |
CAATCTACAGGGTCATCACC+TGG | 0.484929 | 8:-79542765 | MsaT046013.1:intron |
TTAGTGAGGGAGGTTGTTGA+GGG | 0.495882 | 8:+79542380 | None:intergenic |
TTATTTACCATTTGTTTCAG+GGG | 0.508177 | 8:+79542986 | None:intergenic |
GTTTCTGGCTCTTTAAGCAT+AGG | 0.510723 | 8:+79542529 | None:intergenic |
GATACCAATGTCGACTTCAT+CGG | 0.515126 | 8:-79543147 | None:intergenic |
ATTAGTGAGGGAGGTTGTTG+AGG | 0.517348 | 8:+79542379 | None:intergenic |
GCATGCACCCCTGAAACAAA+TGG | 0.517892 | 8:-79542993 | MsaT046013.1:intron |
TAATGGACAAGTTATTGTGT+TGG | 0.534161 | 8:-79542485 | MsaT046013.1:CDS |
GAGAAGAAGGGTAGTTTCGA+AGG | 0.535740 | 8:-79543032 | MsaT046013.1:CDS |
CAGGGGTGCATGCGTTGTGA+AGG | 0.536647 | 8:+79543003 | None:intergenic |
CCTGGTTCATCTTCTCACTC+TGG | 0.547944 | 8:-79542747 | MsaT046013.1:CDS |
CCAGAGTGAGAAGATGAACC+AGG | 0.548191 | 8:+79542747 | None:intergenic |
AGCATGCTGACTCCGCTCCA+TGG | 0.562298 | 8:-79541710 | MsaT046013.1:CDS |
TGCATGCGTTGTGAAGGTTA+AGG | 0.575616 | 8:+79543009 | None:intergenic |
AAATCAATTACCAATTAGTG+AGG | 0.584879 | 8:+79542366 | None:intergenic |
GGTTCTTTGATATGGTGAGT+AGG | 0.584950 | 8:+79541755 | None:intergenic |
TTCTGGAGCAGAGTCAGGAA+AGG | 0.587295 | 8:+79541734 | None:intergenic |
GTTTGTGGGAAATAAATCCA+TGG | 0.589977 | 8:+79542719 | None:intergenic |
TTAGCACCTAACACAAGCCA+TGG | 0.596993 | 8:+79541693 | None:intergenic |
AACTACCCTTCTTCTCCTTG+AGG | 0.603992 | 8:+79543039 | None:intergenic |
TTCTCACTCTGGAATAACCA+TGG | 0.605298 | 8:-79542736 | MsaT046013.1:CDS |
CAATTACCAATTAGTGAGGG+AGG | 0.610131 | 8:+79542370 | None:intergenic |
CAATACCTCAAGGAGAAGAA+GGG | 0.616382 | 8:-79543044 | MsaT046013.1:CDS |
TTCGTCGATTCATGGAGAAG+AGG | 0.618289 | 8:-79542187 | MsaT046013.1:CDS |
ACCTAACACAAGCCATGGAG+CGG | 0.623220 | 8:+79541698 | None:intergenic |
TCTATTGAGCCAATACCTCA+AGG | 0.624878 | 8:-79543054 | MsaT046013.1:CDS |
AATCAATTACCAATTAGTGA+GGG | 0.634671 | 8:+79542367 | None:intergenic |
ATGTCCGATGAAGTCGACAT+TGG | 0.636880 | 8:+79543143 | None:intergenic |
TTACAAGTTTGAGAAAACGT+AGG | 0.650898 | 8:+79543077 | None:intergenic |
CAACAAACTGATAAAGTCGT+CGG | 0.655555 | 8:-79543105 | MsaT046013.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr8 | gene | 79541683 | 79543162 | 79541683 | ID=MsaG046013 |
Chr8 | mRNA | 79541683 | 79543162 | 79541683 | ID=MsaT046013.1;Parent=MsaG046013 |
Chr8 | exon | 79541683 | 79541785 | 79541683 | ID=MsaT046013.1.exon5;Parent=MsaT046013.1 |
Chr8 | CDS | 79541683 | 79541785 | 79541683 | ID=cds.MsaT046013.1;Parent=MsaT046013.1 |
Chr8 | exon | 79542179 | 79542236 | 79542179 | ID=MsaT046013.1.exon4;Parent=MsaT046013.1 |
Chr8 | CDS | 79542179 | 79542236 | 79542179 | ID=cds.MsaT046013.1;Parent=MsaT046013.1 |
Chr8 | exon | 79542377 | 79542561 | 79542377 | ID=MsaT046013.1.exon3;Parent=MsaT046013.1 |
Chr8 | CDS | 79542377 | 79542561 | 79542377 | ID=cds.MsaT046013.1;Parent=MsaT046013.1 |
Chr8 | exon | 79542675 | 79542777 | 79542675 | ID=MsaT046013.1.exon2;Parent=MsaT046013.1 |
Chr8 | CDS | 79542675 | 79542777 | 79542675 | ID=cds.MsaT046013.1;Parent=MsaT046013.1 |
Chr8 | exon | 79542994 | 79543162 | 79542994 | ID=MsaT046013.1.exon1;Parent=MsaT046013.1 |
Chr8 | CDS | 79542994 | 79543162 | 79542994 | ID=cds.MsaT046013.1;Parent=MsaT046013.1 |
Gene Sequence |
Protein sequence |