AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046013


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046013 MtrunA17_Chr4g0070271 92.683 205 11 2 1 204 1 202 9.49e-129 360
MsaG046013 MtrunA17_Chr2g0301131 44.828 261 76 11 1 204 1 250 2.28e-49 161
MsaG046013 MtrunA17_Chr4g0070241 78.125 64 14 0 70 133 1 64 1.02e-28 102
MsaG046013 MtrunA17_Chr5g0399881 36.875 160 69 6 76 204 5 163 1.38e-21 87.0
MsaG046013 MtrunA17_Chr5g0399861 32.927 164 69 5 76 204 5 162 2.84e-21 86.3
MsaG046013 MtrunA17_Chr5g0399851 36.364 132 60 2 94 201 11 142 2.94e-20 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046013 AT1G19180.1 36.328 256 106 10 1 203 1 252 1.27e-35 126
MsaG046013 AT1G19180.3 36.328 256 106 10 1 203 1 252 1.27e-35 126
MsaG046013 AT1G74950.1 36.100 241 75 12 7 180 1 229 5.18e-27 103
MsaG046013 AT1G74950.2 36.052 233 70 12 7 172 1 221 1.23e-23 94.4
MsaG046013 AT1G19180.2 39.098 133 52 5 99 203 55 186 1.26e-18 80.1
MsaG046013 AT1G17380.1 29.353 201 108 5 13 180 7 206 8.79e-14 68.6

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACTTCATCGGACATTTC+TGG 0.184990 8:-79543137 MsaT046013.1:CDS
ATTGTCAATTGTGCAGTTTC+TGG 0.207124 8:+79542514 None:intergenic
CCTTCTTCTCCTTGAGGTAT+TGG 0.218986 8:+79543045 None:intergenic
GGTTGTTGAGGGATCATATT+AGG 0.246891 8:+79542391 None:intergenic
CAACAACCTCCCTCACTAAT+TGG 0.267401 8:-79542376 MsaT046013.1:intron
AGCTTCACTTCGTCGATTCA+TGG 0.304214 8:-79542195 MsaT046013.1:CDS
TCAGGAAAGGTTCTTTGATA+TGG 0.341074 8:+79541747 None:intergenic
TTTATTTACCATTTGTTTCA+GGG 0.363709 8:+79542985 None:intergenic
CGACTTTATCAGTTTGTTGC+CGG 0.376712 8:+79543109 None:intergenic
AGCTCCTCAACTTTCTCAGC+AGG 0.410410 8:+79542454 None:intergenic
TCCGCTCCATGGCTTGTGTT+AGG 0.414480 8:-79541699 MsaT046013.1:CDS
TCATGGAGAAGAGGAAAGAT+AGG 0.451183 8:-79542178 MsaT046013.1:intron
CCAATACCTCAAGGAGAAGA+AGG 0.480824 8:-79543045 MsaT046013.1:CDS
CAATCTACAGGGTCATCACC+TGG 0.484929 8:-79542765 MsaT046013.1:intron
TTAGTGAGGGAGGTTGTTGA+GGG 0.495882 8:+79542380 None:intergenic
TTATTTACCATTTGTTTCAG+GGG 0.508177 8:+79542986 None:intergenic
GTTTCTGGCTCTTTAAGCAT+AGG 0.510723 8:+79542529 None:intergenic
GATACCAATGTCGACTTCAT+CGG 0.515126 8:-79543147 None:intergenic
ATTAGTGAGGGAGGTTGTTG+AGG 0.517348 8:+79542379 None:intergenic
GCATGCACCCCTGAAACAAA+TGG 0.517892 8:-79542993 MsaT046013.1:intron
TAATGGACAAGTTATTGTGT+TGG 0.534161 8:-79542485 MsaT046013.1:CDS
GAGAAGAAGGGTAGTTTCGA+AGG 0.535740 8:-79543032 MsaT046013.1:CDS
CAGGGGTGCATGCGTTGTGA+AGG 0.536647 8:+79543003 None:intergenic
CCTGGTTCATCTTCTCACTC+TGG 0.547944 8:-79542747 MsaT046013.1:CDS
CCAGAGTGAGAAGATGAACC+AGG 0.548191 8:+79542747 None:intergenic
AGCATGCTGACTCCGCTCCA+TGG 0.562298 8:-79541710 MsaT046013.1:CDS
TGCATGCGTTGTGAAGGTTA+AGG 0.575616 8:+79543009 None:intergenic
AAATCAATTACCAATTAGTG+AGG 0.584879 8:+79542366 None:intergenic
GGTTCTTTGATATGGTGAGT+AGG 0.584950 8:+79541755 None:intergenic
TTCTGGAGCAGAGTCAGGAA+AGG 0.587295 8:+79541734 None:intergenic
GTTTGTGGGAAATAAATCCA+TGG 0.589977 8:+79542719 None:intergenic
TTAGCACCTAACACAAGCCA+TGG 0.596993 8:+79541693 None:intergenic
AACTACCCTTCTTCTCCTTG+AGG 0.603992 8:+79543039 None:intergenic
TTCTCACTCTGGAATAACCA+TGG 0.605298 8:-79542736 MsaT046013.1:CDS
CAATTACCAATTAGTGAGGG+AGG 0.610131 8:+79542370 None:intergenic
CAATACCTCAAGGAGAAGAA+GGG 0.616382 8:-79543044 MsaT046013.1:CDS
TTCGTCGATTCATGGAGAAG+AGG 0.618289 8:-79542187 MsaT046013.1:CDS
ACCTAACACAAGCCATGGAG+CGG 0.623220 8:+79541698 None:intergenic
TCTATTGAGCCAATACCTCA+AGG 0.624878 8:-79543054 MsaT046013.1:CDS
AATCAATTACCAATTAGTGA+GGG 0.634671 8:+79542367 None:intergenic
ATGTCCGATGAAGTCGACAT+TGG 0.636880 8:+79543143 None:intergenic
TTACAAGTTTGAGAAAACGT+AGG 0.650898 8:+79543077 None:intergenic
CAACAAACTGATAAAGTCGT+CGG 0.655555 8:-79543105 MsaT046013.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 79541683 79543162 79541683 ID=MsaG046013
Chr8 mRNA 79541683 79543162 79541683 ID=MsaT046013.1;Parent=MsaG046013
Chr8 exon 79541683 79541785 79541683 ID=MsaT046013.1.exon5;Parent=MsaT046013.1
Chr8 CDS 79541683 79541785 79541683 ID=cds.MsaT046013.1;Parent=MsaT046013.1
Chr8 exon 79542179 79542236 79542179 ID=MsaT046013.1.exon4;Parent=MsaT046013.1
Chr8 CDS 79542179 79542236 79542179 ID=cds.MsaT046013.1;Parent=MsaT046013.1
Chr8 exon 79542377 79542561 79542377 ID=MsaT046013.1.exon3;Parent=MsaT046013.1
Chr8 CDS 79542377 79542561 79542377 ID=cds.MsaT046013.1;Parent=MsaT046013.1
Chr8 exon 79542675 79542777 79542675 ID=MsaT046013.1.exon2;Parent=MsaT046013.1
Chr8 CDS 79542675 79542777 79542675 ID=cds.MsaT046013.1;Parent=MsaT046013.1
Chr8 exon 79542994 79543162 79542994 ID=MsaT046013.1.exon1;Parent=MsaT046013.1
Chr8 CDS 79542994 79543162 79542994 ID=cds.MsaT046013.1;Parent=MsaT046013.1
Gene Sequence

>MsaT046013.1

ATGTCGACTTCATCGGACATTTCTGGTTTTTCCGGCAACAAACTGATAAAGTCGTCGGAGAAACCTACGTTTTCTCAAACTTGTAATCTATTGAGCCAATACCTCAAGGAGAAGAAGGGTAGTTTCGAAGGCCTTAACCTTCACAACGCATGCACCCCTGAAACAAATGGGTCATCACCTGGTTCATCTTCTCACTCTGGAATAACCATGGATTTATTTCCCACAAACGTGACACCGAAAAACCTTACAACTATGGATTTTTTCTTTCCAAGAGTTATTAATCCTATGCTTAAAGAGCCAGAAACTGCACAATTGACAATGTTTTATAATGGACAAGTTATTGTGTTGGATGATTTTCCTGCTGAGAAAGTTGAGGAGCTGAAATCATTTGCTAGAACTCAGACTCAGCATTCAGATGTTCCTAATATGATCCCTCAACAACCTCCCTCACTAATTGATATGCCAATTGCTAGAAAAGCTTCACTTCGTCGATTCATGGAGAAGAGGAAAGATAGAGTTTCTGCCTACTCACCATATCAAAGAACCTTTCCTGACTCTGCTCCAGAAAAGCATGCTGACTCCGCTCCATGGCTTGTGTTAGGTGCTAAATCAATGTAA

Protein sequence

>MsaT046013.1

MSTSSDISGFSGNKLIKSSEKPTFSQTCNLLSQYLKEKKGSFEGLNLHNACTPETNGSSPGSSSHSGITMDLFPTNVTPKNLTTMDFFFPRVINPMLKEPETAQLTMFYNGQVIVLDDFPAEKVEELKSFARTQTQHSDVPNMIPQQPPSLIDMPIARKASLRRFMEKRKDRVSAYSPYQRTFPDSAPEKHADSAPWLVLGAKSM*