AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046080 MtrunA17_Chr4g0071051 96.690 423 11 2 1 421 1 422 0.0 839
MsaG046080 MtrunA17_Chr3g0135831 41.226 359 167 9 87 419 33 373 3.66e-77 244
MsaG046080 MtrunA17_Chr7g0260331 47.273 275 124 4 68 321 7 281 2.19e-75 239
MsaG046080 MtrunA17_Chr1g0204741 47.619 252 115 6 64 305 6 250 2.64e-70 225
MsaG046080 MtrunA17_Chr5g0410881 51.707 205 83 3 87 277 21 223 3.46e-65 216
MsaG046080 MtrunA17_Chr1g0185131 47.727 220 106 3 87 304 19 231 1.96e-63 207
MsaG046080 MtrunA17_Chr4g0039891 51.852 216 82 5 99 298 11 220 6.12e-62 207
MsaG046080 MtrunA17_Chr8g0379701 44.444 198 100 2 99 286 11 208 1.38e-51 178
MsaG046080 MtrunA17_Chr6g0467041 41.004 239 118 5 100 335 22 240 1.03e-48 168
MsaG046080 MtrunA17_Chr2g0330821 41.038 212 113 4 94 301 73 276 2.35e-46 163
MsaG046080 MtrunA17_Chr8g0378061 45.604 182 95 2 101 279 23 203 1.07e-45 164
MsaG046080 MtrunA17_Chr8g0392001 47.644 191 86 3 100 276 22 212 2.28e-45 160
MsaG046080 MtrunA17_Chr6g0485571 45.263 190 99 2 100 285 11 199 2.14e-44 157
MsaG046080 MtrunA17_Chr2g0284481 43.784 185 94 3 99 273 14 198 1.33e-41 151
MsaG046080 MtrunA17_Chr3g0134031 60.000 110 43 1 94 203 18 126 7.16e-39 143
MsaG046080 MtrunA17_Chr5g0397881 63.441 93 34 0 98 190 22 114 5.60e-38 140
MsaG046080 MtrunA17_Chr5g0402291 62.766 94 35 0 97 190 6 99 7.90e-38 138
MsaG046080 MtrunA17_Chr5g0441531 64.516 93 33 0 98 190 23 115 5.95e-37 135
MsaG046080 MtrunA17_Chr7g0257331 53.571 112 48 1 91 198 9 120 4.39e-36 132
MsaG046080 MtrunA17_Chr8g0375041 41.975 162 85 3 95 248 4 164 2.32e-34 128
MsaG046080 MtrunA17_Chr1g0203601 58.065 93 39 0 98 190 16 108 6.25e-34 128
MsaG046080 MtrunA17_Chr6g0472421 59.140 93 38 0 98 190 31 123 4.00e-33 127
MsaG046080 MtrunA17_Chr4g0047421 56.701 97 38 1 98 190 31 127 1.09e-31 120
MsaG046080 MtrunA17_Chr1g0179321 63.014 73 27 0 87 159 36 108 6.11e-28 106
MsaG046080 MtrunA17_Chr1g0179311 41.732 127 69 1 160 281 14 140 9.11e-24 101
MsaG046080 MtrunA17_Chr4g0023771 58.571 70 28 1 91 159 6 75 2.77e-20 85.9
MsaG046080 MtrunA17_Chr2g0330841 36.585 123 75 1 160 279 41 163 2.64e-17 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046080 AT2G26150.1 40.950 337 144 8 87 409 31 326 5.36e-76 240
MsaG046080 AT2G26150.4 40.950 337 144 8 87 409 31 326 5.36e-76 240
MsaG046080 AT5G54070.1 52.713 258 108 7 68 322 46 292 9.75e-74 234
MsaG046080 AT3G22830.1 46.586 249 117 4 73 306 34 281 1.33e-73 236
MsaG046080 AT5G16820.1 47.391 230 94 3 70 282 2 221 8.17e-63 210
MsaG046080 AT5G16820.2 47.391 230 94 3 70 282 2 221 8.17e-63 210
MsaG046080 AT3G51910.1 48.792 207 96 3 90 295 19 216 9.06e-63 204
MsaG046080 AT4G17750.1 50.000 216 82 3 98 292 50 260 4.60e-62 208
MsaG046080 AT1G32330.2 47.186 231 106 3 87 301 24 254 7.73e-62 207
MsaG046080 AT1G32330.1 47.186 231 106 3 87 301 24 254 8.44e-62 207
MsaG046080 AT3G63350.1 39.850 266 150 3 73 329 1 265 8.58e-60 196
MsaG046080 AT3G02990.1 51.579 190 81 2 99 277 22 211 1.89e-57 196
MsaG046080 AT5G43840.1 45.503 189 96 1 86 274 5 186 1.76e-54 182
MsaG046080 AT5G03720.2 47.291 203 101 3 86 282 41 243 1.89e-54 186
MsaG046080 AT5G03720.1 47.291 203 101 3 86 282 41 243 1.89e-54 186
MsaG046080 AT1G67970.1 48.108 185 88 2 100 276 19 203 1.38e-49 172
MsaG046080 AT4G13980.1 43.716 183 100 1 101 280 24 206 1.78e-46 166
MsaG046080 AT3G24520.1 41.000 200 102 2 100 299 17 200 5.08e-43 154
MsaG046080 AT4G18880.1 44.865 185 94 2 92 268 7 191 7.70e-38 142
MsaG046080 AT1G46264.1 61.386 101 39 0 90 190 24 124 2.27e-37 139
MsaG046080 AT5G45710.3 41.808 177 96 1 99 268 12 188 2.38e-37 139
MsaG046080 AT5G45710.1 41.808 177 96 1 99 268 12 188 2.38e-37 139
MsaG046080 AT2G41690.1 61.290 93 36 0 98 190 38 130 6.83e-36 132
MsaG046080 AT4G11660.1 61.290 93 36 0 98 190 57 149 2.39e-35 134
MsaG046080 AT4G36990.1 62.366 93 35 0 98 190 12 104 3.40e-35 132
MsaG046080 AT5G62020.1 58.416 101 41 1 98 198 21 120 4.20e-35 132
MsaG046080 AT2G26150.3 29.195 298 136 9 126 409 35 271 3.71e-27 110
MsaG046080 AT2G26150.2 29.195 298 136 9 126 409 35 271 3.71e-27 110
MsaG046080 AT4G18870.1 42.045 88 51 0 100 187 146 233 2.10e-18 85.5
MsaG046080 AT4G18870.1 43.678 87 48 1 101 187 14 99 2.05e-17 82.8
MsaG046080 AT1G77570.1 37.778 90 53 2 101 187 27 116 2.16e-12 65.1
MsaG046080 AT4G19630.1 38.542 96 52 3 97 187 8 101 1.52e-11 62.0

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCAAAAGGCCATTGTTTA+TGG 0.168710 8:-80212218 MsaT046080.1:CDS
GTATTTAGGAAGAAGAATTT+TGG 0.245282 8:+80213398 None:intergenic
GAAACAATCGGGTCAGTTTC+CGG 0.255207 8:+80213471 None:intergenic
CTCCATAAACAATGGCCTTT+TGG 0.280439 8:+80212216 None:intergenic
TGTAACCCTTCCATTGGTTT+AGG 0.283169 8:+80213546 None:intergenic
TTATTATGCTTAAAGTATTT+AGG 0.297550 8:+80213384 None:intergenic
TAATATCTATCTTGAATTAG+AGG 0.304710 8:-80211831 MsaT046080.1:CDS
TGTTATGTCAGAGAAGCTTT+TGG 0.350236 8:-80211897 MsaT046080.1:CDS
TCTGTCACATGCAGGGGTTT+AGG 0.362833 8:-80212546 MsaT046080.1:intron
GATAAGTAAGCCAACAGATT+GGG 0.381656 8:-80211804 MsaT046080.1:CDS
CCTTGTGTTCCAAGCAATCT+AGG 0.385680 8:+80212130 None:intergenic
TTCTGTTGTTGATGAAGAAA+TGG 0.389880 8:-80211867 MsaT046080.1:CDS
CGTTGGGAGTTTGCAAATGA+AGG 0.392464 8:-80212508 MsaT046080.1:CDS
TATTCAACTCACAAATGTTC+AGG 0.406470 8:-80212299 MsaT046080.1:CDS
TAATGTTGATTATAGGAGTC+AGG 0.409936 8:-80212104 MsaT046080.1:CDS
AAACTGACCCGATTGTTTCA+TGG 0.421449 8:-80213467 MsaT046080.1:CDS
TGGATCTGCTAGTGGATTGG+TGG 0.433958 8:-80211780 MsaT046080.1:CDS
GGTGGTTACGACGGTGAAAT+CGG 0.435742 8:-80213789 MsaT046080.1:CDS
TAGCTCTTTACATGACTTGA+GGG 0.437021 8:-80211969 MsaT046080.1:CDS
GTTTGAAATGGTGGAGGATC+CGG 0.440036 8:-80213490 MsaT046080.1:CDS
GTTAAAAGACCTAGATTGCT+TGG 0.444562 8:-80212139 MsaT046080.1:CDS
AAGGTACTAATGTTGATTAT+AGG 0.444857 8:-80212111 MsaT046080.1:CDS
GCACTGTCGTTGAATAGCTC+TGG 0.449080 8:+80213732 None:intergenic
AAATATGAACACTGGTAGAA+TGG 0.455673 8:-80212029 MsaT046080.1:CDS
GAAGCACTCCATGAAACAAT+CGG 0.466108 8:+80213459 None:intergenic
TCTCAAGAAGACGTTTGAAA+TGG 0.467110 8:-80213502 MsaT046080.1:CDS
TGATAAGTAAGCCAACAGAT+TGG 0.468059 8:-80211805 MsaT046080.1:CDS
TCCTCACCAAAATGGCCAAA+AGG 0.470362 8:-80212231 MsaT046080.1:CDS
TCTTCCATGGGAGTAGAAAC+AGG 0.475802 8:+80212001 None:intergenic
GAAGGTTGATTCAGACCGTT+GGG 0.482934 8:-80212524 MsaT046080.1:CDS
CAAATGAAGGATTTCAAGCA+GGG 0.484602 8:-80212495 MsaT046080.1:CDS
GACTTACATAGGTGTTGAGC+TGG 0.485810 8:+80213343 None:intergenic
ATTTGTTGAAGAACATAAGA+AGG 0.488953 8:-80212465 MsaT046080.1:CDS
ACCTCATGTAACCCTTCCAT+TGG 0.493568 8:+80213540 None:intergenic
GGGTGGATCTGCTAGTGGAT+TGG 0.502731 8:-80211783 MsaT046080.1:CDS
GCAAATGAAGGATTTCAAGC+AGG 0.502811 8:-80212496 MsaT046080.1:CDS
ATTCAAAAGATTAAGCGAAA+AGG 0.508398 8:-80212187 MsaT046080.1:CDS
TTACATGACTTGAGGGCTTG+TGG 0.510182 8:-80211962 MsaT046080.1:CDS
AACAAAATGGCGGTGGCACA+CGG 0.510707 8:-80213810 None:intergenic
ACTTTGCAATCTGAATTGAA+TGG 0.510922 8:-80212064 MsaT046080.1:CDS
CAAGAAGACGTTTGAAATGG+TGG 0.512314 8:-80213499 MsaT046080.1:CDS
GTTTCTACTCCCATGGAAGA+TGG 0.512936 8:-80211998 MsaT046080.1:CDS
CGCAATTCATATCAAAGAAG+AGG 0.515252 8:-80213613 MsaT046080.1:CDS
GGAAGGTTGATTCAGACCGT+TGG 0.515312 8:-80212525 MsaT046080.1:CDS
GTAGCTCTTTACATGACTTG+AGG 0.518635 8:-80211970 MsaT046080.1:CDS
TCAAACGTCTTCTTGAGAAA+CGG 0.525722 8:+80213507 None:intergenic
AATTAGAAATTCTGAAGCTA+AGG 0.528634 8:-80212342 MsaT046080.1:CDS
GCTACACAGTCCATCTTCCA+TGG 0.535967 8:+80211988 None:intergenic
GGATCTGCTAGTGGATTGGT+GGG 0.540365 8:-80211779 MsaT046080.1:CDS
ACTCAGGTTTCCTAAACCAA+TGG 0.540511 8:-80213556 MsaT046080.1:CDS
ACCACCTGTTTCTACTCCCA+TGG 0.552610 8:-80212005 MsaT046080.1:CDS
AGGTTTCCTAAACCAATGGA+AGG 0.554746 8:-80213552 MsaT046080.1:CDS
TCCACTTCAGATTCAACGCA+AGG 0.559576 8:+80212394 None:intergenic
CTTCATTTGCAAACTCCCAA+CGG 0.561522 8:+80212509 None:intergenic
CCTAGATTGCTTGGAACACA+AGG 0.567220 8:-80212130 MsaT046080.1:CDS
ACAAATAAACAAAATGGCGG+TGG 0.567551 8:-80213817 None:intergenic
TCACATGCAGGGGTTTAGGA+AGG 0.572288 8:-80212542 MsaT046080.1:intron
ATAAGTAAGCCAACAGATTG+GGG 0.574399 8:-80211803 MsaT046080.1:CDS
AAGCACTCCATGAAACAATC+GGG 0.577813 8:+80213460 None:intergenic
AAGTATAACAAACTGCATCA+AGG 0.578236 8:-80212436 MsaT046080.1:CDS
AATGTTGATTATAGGAGTCA+GGG 0.582952 8:-80212103 MsaT046080.1:CDS
TTTCCTGAAAATATGAACAC+TGG 0.585214 8:-80212037 MsaT046080.1:CDS
CTAACGCTTCATCTTCACTC+AGG 0.586633 8:-80213572 MsaT046080.1:CDS
GCAGATCCACCCCAATCTGT+TGG 0.588399 8:+80211794 None:intergenic
GTCTTCTTGAGAAACGGTGG+TGG 0.589533 8:+80213513 None:intergenic
ATGGAAGGGTTACATGAGGT+TGG 0.601818 8:-80213537 MsaT046080.1:CDS
TCCTTGCGTTGAATCTGAAG+TGG 0.604555 8:-80212395 MsaT046080.1:CDS
GAAGAGGTTCTTGAAGAAGA+CGG 0.609483 8:-80213597 MsaT046080.1:CDS
CTACACAGTCCATCTTCCAT+GGG 0.610941 8:+80211989 None:intergenic
TTCGGTTGAAACGTCGACGT+AGG 0.619310 8:+80213692 None:intergenic
ATTATCTGCTCCATAAACAA+TGG 0.622243 8:+80212208 None:intergenic
GATTGGGGTGGATCTGCTAG+TGG 0.632406 8:-80211788 MsaT046080.1:CDS
GGTTTCCTAAACCAATGGAA+GGG 0.633308 8:-80213551 MsaT046080.1:CDS
GAAGACGTTTGAAATGGTGG+AGG 0.633963 8:-80213496 MsaT046080.1:CDS
CATGACTTGAGGGCTTGTGG+TGG 0.643012 8:-80211959 MsaT046080.1:CDS
GTGGCACACGGTGGTTACGA+CGG 0.645161 8:-80213798 MsaT046080.1:CDS
AACGTCTTCTTGAGAAACGG+TGG 0.656391 8:+80213510 None:intergenic
AGTAAGCCAACAGATTGGGG+TGG 0.660771 8:-80211800 MsaT046080.1:CDS
ACCAATGGAAGGGTTACATG+AGG 0.667282 8:-80213541 MsaT046080.1:CDS
GGTTACGACGGTGAAATCGG+TGG 0.688203 8:-80213786 MsaT046080.1:CDS
AGTTGATGCTAATATAGACA+TGG 0.689403 8:-80212161 MsaT046080.1:CDS
TATAACAAACTGCATCAAGG+AGG 0.715319 8:-80212433 MsaT046080.1:CDS
AAAATGGCGGTGGCACACGG+TGG 0.747694 8:-80213807 MsaT046080.1:CDS
TCCATGGGAGTAGAAACAGG+TGG 0.762840 8:+80212004 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 80211766 80213826 80211766 ID=MsaG046080
Chr8 mRNA 80211766 80213826 80211766 ID=MsaT046080.1;Parent=MsaG046080
Chr8 exon 80211766 80212554 80211766 ID=MsaT046080.1.exon2;Parent=MsaT046080.1
Chr8 CDS 80211766 80212554 80211766 ID=cds.MsaT046080.1;Parent=MsaT046080.1
Chr8 exon 80213350 80213826 80213350 ID=MsaT046080.1.exon1;Parent=MsaT046080.1
Chr8 CDS 80213350 80213826 80213350 ID=cds.MsaT046080.1;Parent=MsaT046080.1
Gene Sequence

>MsaT046080.1

ATGGCGGTGGCACACGGTGGTTACGACGGTGAAATCGGTGGCTACAATTCTCGCCTAATCCCATCAATAAAACCAGAGCTATTCAACGACAGTGCAAATTCAAGCTCTTACACCTACGTCGACGTTTCAACCGAAAGAAAAGAATCAGTACTAACCGAAGAAGAAGAAGAAGAAGGTGCTACAGCTTCTCACGCAATTCATATCAAAGAAGAGGTTCTTGAAGAAGACGGTGCTAACGCTTCATCTTCACTCAGGTTTCCTAAACCAATGGAAGGGTTACATGAGGTTGGTCCACCACCGTTTCTCAAGAAGACGTTTGAAATGGTGGAGGATCCGGAAACTGACCCGATTGTTTCATGGAGTGCTTCTCGTGATAGTTTTGTTGTTTGGGATTCTCATGAATTCTCCAAAATTCTTCTTCCTAAATACTTTAAGCATAATAATTTTTCTAGCTTCATTCGCCAGCTCAACACCTATGGGTTTAGGAAGGTTGATTCAGACCGTTGGGAGTTTGCAAATGAAGGATTTCAAGCAGGGAAAAAGCATTTGTTGAAGAACATAAGAAGGAGAAGCAAGTATAACAAACTGCATCAAGGAGGATTCAATTTGATGAATCCTTGCGTTGAATCTGAAGTGGAGAAACTGAAAAAAGATCAGAACATGTTGAAATTAGAAATTCTGAAGCTAAGGCAACAACAAGAGAATTCAAATATTCAACTCACAAATGTTCAGGAGCGAGTTCAGCGAGCAGAATCGAAGCAATATCAGATGCTATTTTTCCTCACCAAAATGGCCAAAAGGCCATTGTTTATGGAGCAGATAATTCAAAAGATTAAGCGAAAAGGAGAAGTTGATGCTAATATAGACATGGTTAAAAGACCTAGATTGCTTGGAACACAAGGTACTAATGTTGATTATAGGAGTCAGGGTCGTGAACAATTTGCTACTTTGCAATCTGAATTGAATGGACTTTTTCCTGAAAATATGAACACTGGTAGAATGGAACCACCTGTTTCTACTCCCATGGAAGATGGACTGTGTAGCTCTTTACATGACTTGAGGGCTTGTGGTGGTTCTAGACAAAGTGTTCAAGATGCTACATCTGCATATCATGTTATGTCAGAGAAGCTTTTGGGGGAAAATTCTGTTGTTGATGAAGAAATGGATGTGAATGACTCTAATATCTATCTTGAATTAGAGGATTTGATAAGTAAGCCAACAGATTGGGGTGGATCTGCTAGTGGATTGGTGGGACAAACTAGTTGA
Protein sequence

>MsaT046080.1

MAVAHGGYDGEIGGYNSRLIPSIKPELFNDSANSSSYTYVDVSTERKESVLTEEEEEEGATASHAIHIKEEVLEEDGANASSSLRFPKPMEGLHEVGPPPFLKKTFEMVEDPETDPIVSWSASRDSFVVWDSHEFSKILLPKYFKHNNFSSFIRQLNTYGFRKVDSDRWEFANEGFQAGKKHLLKNIRRRSKYNKLHQGGFNLMNPCVESEVEKLKKDQNMLKLEILKLRQQQENSNIQLTNVQERVQRAESKQYQMLFFLTKMAKRPLFMEQIIQKIKRKGEVDANIDMVKRPRLLGTQGTNVDYRSQGREQFATLQSELNGLFPENMNTGRMEPPVSTPMEDGLCSSLHDLRACGGSRQSVQDATSAYHVMSEKLLGENSVVDEEMDVNDSNIYLELEDLISKPTDWGGSASGLVGQTS*