AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046108


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046108 MtrunA17_Chr4g0071411 91.457 199 13 2 1 199 1 195 5.16e-122 344
MsaG046108 MtrunA17_Chr8g0385491 83.495 103 13 2 1 103 83 181 1.95e-45 148
MsaG046108 MtrunA17_Chr5g0400781 71.287 101 25 1 80 180 115 211 1.99e-39 135
MsaG046108 MtrunA17_Chr5g0399491 57.258 124 46 1 61 184 112 228 3.41e-39 135
MsaG046108 MtrunA17_Chr4g0055851 61.261 111 37 1 80 184 102 212 7.10e-38 132
MsaG046108 MtrunA17_Chr8g0335131 70.213 94 28 0 91 184 145 238 5.08e-37 130
MsaG046108 MtrunA17_Chr1g0175651 54.198 131 54 2 50 180 118 242 1.93e-36 129
MsaG046108 MtrunA17_Chr7g0258831 52.672 131 57 1 50 180 123 248 2.68e-36 128
MsaG046108 MtrunA17_Chr4g0054421 70.000 90 27 0 91 180 126 215 4.05e-36 127
MsaG046108 MtrunA17_Chr2g0308611 61.765 102 39 0 79 180 187 288 3.25e-35 126
MsaG046108 MtrunA17_Chr1g0151971 68.889 90 28 0 91 180 129 218 2.05e-34 123
MsaG046108 MtrunA17_Chr2g0299031 45.355 183 82 3 11 191 15 181 1.08e-33 119
MsaG046108 MtrunA17_Chr4g0044091 50.000 118 54 2 86 200 59 174 5.51e-30 109
MsaG046108 MtrunA17_Chr3g0135331 55.963 109 43 2 86 190 139 246 1.12e-27 106
MsaG046108 MtrunA17_Chr7g0217111 48.864 88 45 0 91 178 79 166 2.48e-19 83.2
MsaG046108 MtrunA17_Chr3g0119291 47.368 95 43 1 92 179 59 153 4.08e-19 82.4
MsaG046108 MtrunA17_Chr8g0362751 48.936 94 41 1 92 178 89 182 5.56e-19 82.4
MsaG046108 MtrunA17_Chr5g0415941 46.809 94 43 1 92 178 87 180 2.45e-18 81.3
MsaG046108 MtrunA17_Chr7g0265521 42.453 106 61 0 73 178 44 149 2.46e-18 79.7
MsaG046108 MtrunA17_Chr6g0450991 48.387 93 41 1 92 177 67 159 4.11e-18 80.1
MsaG046108 MtrunA17_Chr8g0362151 46.591 88 47 0 92 179 77 164 1.82e-17 78.2
MsaG046108 MtrunA17_Chr6g0453021 47.872 94 42 1 92 178 96 189 1.83e-17 78.2
MsaG046108 MtrunA17_Chr8g0381741 45.263 95 45 1 91 178 53 147 2.62e-17 78.2
MsaG046108 MtrunA17_Chr5g0416391 45.455 88 48 0 92 179 77 164 7.22e-17 76.6
MsaG046108 MtrunA17_Chr1g0179581 38.318 107 65 1 68 174 5 110 1.84e-15 69.3
MsaG046108 MtrunA17_Chr3g0123691 40.385 104 53 2 82 178 52 153 2.05e-15 72.8
MsaG046108 MtrunA17_Chr4g0060031 45.161 93 44 1 92 177 95 187 8.73e-15 71.6
MsaG046108 MtrunA17_Chr8g0346251 34.266 143 80 2 65 193 7 149 1.20e-13 67.4
MsaG046108 MtrunA17_Chr3g0127511 42.857 84 48 0 95 178 41 124 1.75e-12 64.3
MsaG046108 MtrunA17_Chr5g0403691 47.297 74 39 0 85 158 46 119 6.97e-11 59.3
MsaG046108 MtrunA17_Chr5g0403701 47.297 74 39 0 85 158 46 119 8.11e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046108 AT4G37790.1 42.523 214 89 4 1 180 1 214 4.46e-40 138
MsaG046108 AT3G60390.1 63.889 108 38 1 78 184 147 254 3.86e-39 136
MsaG046108 AT5G06710.1 61.165 103 40 0 78 180 176 278 2.58e-37 132
MsaG046108 AT2G22800.1 42.079 202 94 4 1 180 1 201 1.63e-36 129
MsaG046108 AT4G16780.1 61.111 108 38 2 81 188 119 222 6.57e-36 127
MsaG046108 AT2G44910.1 64.894 94 33 0 91 184 162 255 1.92e-34 124
MsaG046108 AT4G17460.1 63.830 94 34 0 91 184 134 227 3.82e-33 120
MsaG046108 AT5G47370.1 63.830 94 34 0 91 184 129 222 4.53e-33 120
MsaG046108 AT1G70920.2 44.094 127 66 1 67 188 14 140 4.26e-28 104
MsaG046108 AT1G70920.1 44.094 127 66 1 67 188 44 170 5.59e-28 105
MsaG046108 AT2G01430.1 49.167 120 56 1 78 192 120 239 9.09e-28 106
MsaG046108 AT5G06710.4 59.155 71 29 0 73 143 171 241 6.56e-22 90.5
MsaG046108 AT1G26960.1 40.000 120 71 1 61 179 39 158 1.07e-18 81.6
MsaG046108 AT1G69780.1 48.864 88 45 0 92 179 85 172 1.51e-17 79.0
MsaG046108 AT3G01470.1 48.387 93 41 2 92 177 68 160 4.56e-17 77.4
MsaG046108 AT2G01430.2 48.864 88 38 2 78 160 120 205 5.37e-17 76.3
MsaG046108 AT3G01220.1 50.633 79 39 0 92 170 87 165 8.96e-17 77.0
MsaG046108 AT5G15150.1 48.936 94 41 1 92 178 115 208 2.06e-16 76.3
MsaG046108 AT2G46680.2 43.158 95 50 1 83 177 27 117 5.62e-16 74.3
MsaG046108 AT2G46680.1 43.158 95 50 1 83 177 27 117 6.19e-16 74.3
MsaG046108 AT5G65310.1 43.158 95 47 1 92 179 72 166 1.65e-15 73.6
MsaG046108 AT5G03790.1 42.708 96 55 0 85 180 70 165 1.75e-15 72.8
MsaG046108 AT5G65310.2 43.158 95 47 1 92 179 54 148 1.90e-15 73.2
MsaG046108 AT2G22430.1 44.211 95 46 1 92 179 62 156 5.17e-15 72.4
MsaG046108 AT4G40060.1 44.086 93 48 1 92 180 59 151 6.68e-15 72.0
MsaG046108 AT5G03790.2 44.444 90 50 0 85 174 70 159 3.55e-14 68.2
MsaG046108 AT3G61890.1 40.964 83 49 0 95 177 33 115 2.36e-13 66.6
MsaG046108 AT2G36610.1 38.596 114 62 2 75 180 54 167 7.98e-13 64.3
MsaG046108 AT4G36740.2 40.625 96 57 0 83 178 47 142 3.65e-12 63.2
MsaG046108 AT4G36740.1 40.625 96 57 0 83 178 46 141 3.79e-12 63.2
MsaG046108 AT5G53980.1 43.333 90 49 2 90 178 9 97 4.36e-12 62.0
MsaG046108 AT2G18550.1 38.679 106 63 1 73 178 44 147 4.93e-12 62.8
MsaG046108 AT5G66700.1 45.833 72 39 0 92 163 71 142 1.74e-11 61.6
MsaG046108 AT1G27045.4 42.391 92 53 0 92 183 68 159 8.03e-11 60.1

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTGAAAGATTCAATT+TGG 0.267895 8:-80585458 MsaT046108.1:CDS
ACGTTCATAGGTTCTCCTTT+TGG 0.352374 8:+80585502 None:intergenic
TTGAGGTTTGGTTTCAGAAC+AGG 0.362659 8:-80584904 MsaT046108.1:CDS
GTCTTGTCTTTAACCTCAAT+TGG 0.365222 8:+80584930 None:intergenic
TTGTTGTTGTGTACGTTCAT+AGG 0.371543 8:+80585490 None:intergenic
TCTACGATCGTTGTTGTTGT+TGG 0.376794 8:+80585384 None:intergenic
AAGATTAAAGAAGGAATTGC+AGG 0.404223 8:-80584542 MsaT046108.1:CDS
AGACAAGACAAATTGAGGTT+TGG 0.409339 8:-80584916 MsaT046108.1:CDS
ACAGGTTTATGTCTAGGTCT+TGG 0.420666 8:-80585610 MsaT046108.1:CDS
TTATGTCTAGGTCTTGGAAT+AGG 0.424289 8:-80585604 MsaT046108.1:CDS
TTGCAGGAACTCAAAGTTGT+TGG 0.456628 8:-80584526 MsaT046108.1:CDS
TAGCACTTGCTGACCAATTG+AGG 0.458161 8:-80584943 MsaT046108.1:CDS
GCATTCTTCATCACTTTCCA+TGG 0.470187 8:+80585636 None:intergenic
GTTAAAGACAAGACAAATTG+AGG 0.472426 8:-80584921 MsaT046108.1:CDS
ATACCGGATTAAGAGTGTTA+TGG 0.475608 8:+80585305 None:intergenic
AGTGATGAAGAATGCAATAC+AGG 0.485322 8:-80585628 MsaT046108.1:CDS
GCTCCATAACACTCTTAATC+CGG 0.495490 8:-80585308 MsaT046108.1:intron
AAGAGTGTTATGGAGCTTGA+AGG 0.507942 8:+80585315 None:intergenic
TTGGACAAAGCTCAAAAGCT+AGG 0.524782 8:+80585521 None:intergenic
GACTAAGCTGAAGCAGATAG+AGG 0.526640 8:-80584617 MsaT046108.1:CDS
TGAGAACAAAAGATTAAAGA+AGG 0.527592 8:-80584551 MsaT046108.1:CDS
TGCAATACAGGTTTATGTCT+AGG 0.537810 8:-80585616 MsaT046108.1:CDS
CTTGAAGGTGCTCTCAAGCA+TGG 0.539476 8:+80585330 None:intergenic
GATGAACGTTGAGGGCATAA+CGG 0.572104 8:+80584496 None:intergenic
GGTTTCAGAACAGGAGAGCA+AGG 0.572572 8:-80584895 MsaT046108.1:intron
ATTAGTGAATAAATCTGTGA+TGG 0.573054 8:-80585557 MsaT046108.1:CDS
TCTTGGAATAGGTGCAACAA+AGG 0.613459 8:-80585593 MsaT046108.1:CDS
TAAGCTGAAGCAGATAGAGG+TGG 0.656332 8:-80584614 MsaT046108.1:CDS
AAAGAAACTGAGGCTAACAA+AGG 0.656758 8:-80585362 MsaT046108.1:CDS
TCAAAAGCTAGGTCAAAACA+AGG 0.669862 8:+80585532 None:intergenic
ACGTTCATCATCAAAACCAG+TGG 0.688173 8:-80584482 MsaT046108.1:intron
ACGATCGTAGAAAGAAACTG+AGG 0.720750 8:-80585372 MsaT046108.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 80583122 80585656 80583122 ID=MsaG046108
Chr8 mRNA 80583122 80585656 80583122 ID=MsaT046108.1;Parent=MsaG046108
Chr8 exon 80583122 80583139 80583122 ID=MsaT046108.1.exon4;Parent=MsaT046108.1
Chr8 CDS 80583122 80583139 80583122 ID=cds.MsaT046108.1;Parent=MsaT046108.1
Chr8 exon 80584483 80584639 80584483 ID=MsaT046108.1.exon3;Parent=MsaT046108.1
Chr8 CDS 80584483 80584639 80584483 ID=cds.MsaT046108.1;Parent=MsaT046108.1
Chr8 exon 80584896 80584975 80584896 ID=MsaT046108.1.exon2;Parent=MsaT046108.1
Chr8 CDS 80584896 80584975 80584896 ID=cds.MsaT046108.1;Parent=MsaT046108.1
Chr8 exon 80585309 80585656 80585309 ID=MsaT046108.1.exon1;Parent=MsaT046108.1
Chr8 CDS 80585309 80585656 80585309 ID=cds.MsaT046108.1;Parent=MsaT046108.1
Gene Sequence

>MsaT046108.1

ATGGAAAGTGATGAAGAATGCAATACAGGTTTATGTCTAGGTCTTGGAATAGGTGCAACAAAGGTGAAGAAGCAGAAATTAGTGAATAAATCTGTGATGGCACCTTGTTTTGACCTAGCTTTTGAGCTTTGTCCAAAAGGAGAACCTATGAACGTACACAACAACAACAACAACAGCAAAGGTGAAAGATTCAATTTGGAGAGACACCAATATTATCAAAATGTAACGTGTAGCACTGATTCTGATAACACCAACAACAACAACGATCGTAGAAAGAAACTGAGGCTAACAAAGGAGCAATCATCCATGCTTGAGAGCACCTTCAAGCTCCATAACACTCTTAATCCGGTTCAGAAAATAGCACTTGCTGACCAATTGAGGTTAAAGACAAGACAAATTGAGGTTTGGTTTCAGAACAGGAGAGCAAGGACTAAGCTGAAGCAGATAGAGGTGGATTATGAGTTGTTGAAAAAACATTGTCAAAATCTAAGTGATGAGAACAAAAGATTAAAGAAGGAATTGCAGGAACTCAAAGTTGTTGGACAATTTCCGTTATGCCCTCAACGTTCATCATCAAAACCAGTGATTGGAGAGTCGATATAA

Protein sequence

>MsaT046108.1

MESDEECNTGLCLGLGIGATKVKKQKLVNKSVMAPCFDLAFELCPKGEPMNVHNNNNNSKGERFNLERHQYYQNVTCSTDSDNTNNNNDRRKKLRLTKEQSSMLESTFKLHNTLNPVQKIALADQLRLKTRQIEVWFQNRRARTKLKQIEVDYELLKKHCQNLSDENKRLKKELQELKVVGQFPLCPQRSSSKPVIGESI*