AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046195

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046195 MtrunA17_Chr4g0072571 97.656 128 3 0 1 128 42 169 9.94e-91 259
MsaG046195 MtrunA17_Chr8g0376021 51.852 81 34 2 1 79 59 136 5.20e-19 79.0
MsaG046195 MtrunA17_Chr2g0333741 35.632 174 62 6 1 128 78 247 1.15e-18 78.6
MsaG046195 MtrunA17_Chr1g0161901 35.443 158 66 6 4 128 97 251 2.12e-18 77.8
MsaG046195 MtrunA17_Chr5g0434121 67.273 55 15 1 4 55 87 141 5.84e-18 76.6
MsaG046195 MtrunA17_Chr3g0110091 64.286 56 19 1 3 58 85 139 3.74e-17 74.3
MsaG046195 MtrunA17_Chr4g0001081 67.347 49 14 1 1 47 64 112 1.04e-16 73.2
MsaG046195 MtrunA17_Chr1g0149811 64.583 48 17 0 1 48 91 138 1.81e-16 73.2
MsaG046195 MtrunA17_Chr3g0137111 73.684 38 10 0 2 39 70 107 6.40e-15 68.2
MsaG046195 MtrunA17_Chr7g0254221 74.286 35 9 0 4 38 33 67 2.92e-13 62.8
MsaG046195 MtrunA17_Chr1g0207691 70.588 34 10 0 4 37 64 97 3.51e-12 60.5
MsaG046195 MtrunA17_Chr7g0251581 75.758 33 8 0 4 36 39 71 4.64e-12 60.1
MsaG046195 MtrunA17_Chr7g0222361 29.936 157 78 5 3 128 47 202 1.19e-11 59.3
MsaG046195 MtrunA17_Chr7g0225121 31.159 138 78 3 3 128 80 212 3.57e-11 58.2
MsaG046195 MtrunA17_Chr3g0079671 61.538 39 15 0 2 40 96 134 7.05e-11 58.2
MsaG046195 MtrunA17_Chr4g0053691 67.647 34 11 0 3 36 66 99 9.10e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046195 AT5G57520.1 47.287 129 43 6 1 128 46 150 1.96e-22 86.7
MsaG046195 AT5G10970.1 45.714 105 46 4 2 106 100 193 2.21e-19 81.3
MsaG046195 AT5G25160.1 54.545 88 25 5 2 85 57 133 1.01e-18 79.0
MsaG046195 AT1G66140.1 75.000 44 11 0 1 44 80 123 2.27e-17 75.9
MsaG046195 AT1G80730.1 54.054 74 30 1 2 71 64 137 4.98e-17 74.3
MsaG046195 AT1G24625.1 78.947 38 8 0 4 41 57 94 3.66e-16 71.6
MsaG046195 AT5G14010.1 31.783 129 81 2 4 128 36 161 5.60e-13 62.4
MsaG046195 AT5G01860.1 53.061 49 19 1 4 52 65 109 3.01e-12 61.2

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGGAAGGTGTGGCTTCT+TGG 0.263748 8:+81632390 MsaT046195.1:CDS
TTTCAAGCTTGTGAGCATTT+TGG 0.263848 8:-81632172 None:intergenic
GAGAAGTTTGAAGGGTGTTC+TGG 0.318303 8:-81632257 None:intergenic
GTTTAGTTATGGAAATTCTA+AGG 0.345981 8:+81632373 MsaT046195.1:CDS
TTCTCTACTAATTACCATCT+TGG 0.347245 8:+81632275 MsaT046195.1:CDS
AGAAAAGATCAGTTTAGTTA+TGG 0.369153 8:+81632362 MsaT046195.1:CDS
TCATCACATGACCTTGTCTA+TGG 0.389808 8:-81632328 None:intergenic
GCCAACTTGATTTGTCCTTA+AGG 0.395324 8:+81632459 MsaT046195.1:CDS
CATTTATAATCAGAGCCTTA+AGG 0.403759 8:-81632474 None:intergenic
TAGTACTTCTGAAAATGTTC+AGG 0.411786 8:+81632427 MsaT046195.1:CDS
AGGTGTGGCTTCTTGGTCAA+GGG 0.430940 8:+81632397 MsaT046195.1:CDS
TAATTAGTAGAGAAGTTTGA+AGG 0.473837 8:-81632266 None:intergenic
AAGGTGTGGCTTCTTGGTCA+AGG 0.476344 8:+81632396 MsaT046195.1:CDS
AGTTATGGAAATTCTAAGGA+AGG 0.495820 8:+81632377 MsaT046195.1:CDS
GGACACTTGCAAAGAAGAGT+AGG 0.507806 8:+81632195 MsaT046195.1:CDS
AAAATGCTCACAAGCTTGAA+AGG 0.509103 8:+81632174 MsaT046195.1:CDS
AATTACCATCTTGGAAGTCA+TGG 0.516260 8:+81632284 MsaT046195.1:CDS
CATTTCCATGACTTCCAAGA+TGG 0.523980 8:-81632289 None:intergenic
AATTAGTAGAGAAGTTTGAA+GGG 0.551396 8:-81632265 None:intergenic
TGGAAATTCTAAGGAAGGTG+TGG 0.568793 8:+81632382 MsaT046195.1:CDS
GCCTTAAGGACAAATCAAGT+TGG 0.573380 8:-81632460 None:intergenic
GACACTTGCAAAGAAGAGTA+GGG 0.576451 8:+81632196 MsaT046195.1:CDS
CATCTTCATCTATGGAGCAA+AGG 0.583885 8:+81632090 None:intergenic
GGTGTGGCTTCTTGGTCAAG+GGG 0.586234 8:+81632398 MsaT046195.1:CDS
TATAGCTCTCAAGCTTTGGG+AGG 0.587863 8:+81632146 MsaT046195.1:CDS
TACTTCTGAAAATGTTCAGG+AGG 0.608835 8:+81632430 MsaT046195.1:CDS
TTCAGCATAAGAAGATTGCA+TGG 0.617834 8:-81632231 None:intergenic
ATAGACAAGGTCATGTGATG+AGG 0.622400 8:+81632330 MsaT046195.1:CDS
CTTGACAACAACCATAGACA+AGG 0.646735 8:+81632317 MsaT046195.1:CDS
CAAGGTCATGTGATGAGGCA+TGG 0.652675 8:+81632335 MsaT046195.1:CDS
GGTCATGTGATGAGGCATGG+TGG 0.708373 8:+81632338 MsaT046195.1:CDS
AGCATAAGAAGATTGCATGG+CGG 0.769594 8:-81632228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 81632101 81632487 81632101 ID=MsaG046195
Chr8 mRNA 81632101 81632487 81632101 ID=MsaT046195.1;Parent=MsaG046195
Chr8 exon 81632101 81632487 81632101 ID=MsaT046195.1.exon1;Parent=MsaT046195.1
Chr8 CDS 81632101 81632487 81632101 ID=cds.MsaT046195.1;Parent=MsaT046195.1
Gene Sequence

>MsaT046195.1

ATGGAGCAAAGGATATTCTCATGCAATTATTGTCAGAGAAAGTTTTATAGCTCTCAAGCTTTGGGAGGACACCAAAATGCTCACAAGCTTGAAAGGACACTTGCAAAGAAGAGTAGGGAGATGAGTTCCGCCATGCAATCTTCTTATGCTGAATTACCAGAACACCCTTCAAACTTCTCTACTAATTACCATCTTGGAAGTCATGGAAATGCTCATCTTGACAACAACCATAGACAAGGTCATGTGATGAGGCATGGTGGAAGAAAAGATCAGTTTAGTTATGGAAATTCTAAGGAAGGTGTGGCTTCTTGGTCAAGGGGTTATAATAGTACTTCTGAAAATGTTCAGGAGGATATTAGCCAACTTGATTTGTCCTTAAGGCTCTGA
Protein sequence

>MsaT046195.1

MEQRIFSCNYCQRKFYSSQALGGHQNAHKLERTLAKKSREMSSAMQSSYAELPEHPSNFSTNYHLGSHGNAHLDNNHRQGHVMRHGGRKDQFSYGNSKEGVASWSRGYNSTSENVQEDISQLDLSLRL*