AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046320 MtrunA17_Chr4g0074001 88.996 518 28 2 1 490 1 517 0.0 888
MsaG046320 MtrunA17_Chr7g0218081 40.136 294 133 8 157 427 226 499 1.12e-50 180
MsaG046320 MtrunA17_Chr3g0101131 36.842 342 166 12 87 397 156 478 3.16e-49 178
MsaG046320 MtrunA17_Chr5g0445311 37.175 269 155 5 128 394 170 426 5.45e-49 176
MsaG046320 MtrunA17_Chr5g0445311 53.731 67 30 1 331 397 199 264 3.56e-15 78.2
MsaG046320 MtrunA17_Chr5g0418191 40.000 250 137 7 149 394 142 382 1.72e-48 172
MsaG046320 MtrunA17_Chr5g0418191 54.688 64 28 1 333 396 152 214 9.01e-16 79.3
MsaG046320 MtrunA17_Chr4g0042831 37.261 314 140 11 83 394 82 340 4.43e-47 167
MsaG046320 MtrunA17_Chr4g0042831 44.444 90 44 2 307 395 124 208 7.16e-13 70.1
MsaG046320 MtrunA17_Chr7g0249311 38.652 282 123 7 158 394 178 454 7.08e-45 167
MsaG046320 MtrunA17_Chr7g0249311 44.898 98 52 2 315 412 163 258 4.30e-16 81.3
MsaG046320 MtrunA17_Chr3g0079691 35.986 289 137 7 151 394 272 557 2.41e-44 166
MsaG046320 MtrunA17_Chr3g0079691 49.333 75 33 2 328 398 272 345 5.56e-16 80.9
MsaG046320 MtrunA17_Chr3g0106491 39.526 253 129 5 151 394 190 427 3.57e-44 162
MsaG046320 MtrunA17_Chr3g0106491 52.239 67 31 1 331 397 197 262 8.61e-16 79.7
MsaG046320 MtrunA17_Chr5g0432161 39.286 252 127 6 158 394 243 483 1.26e-43 162
MsaG046320 MtrunA17_Chr7g0263531 35.341 249 156 3 146 392 176 421 2.96e-43 158
MsaG046320 MtrunA17_Chr7g0263531 47.368 76 39 1 331 406 188 262 2.80e-17 84.0
MsaG046320 MtrunA17_Chr7g0243891 38.710 248 123 7 158 398 215 440 6.77e-42 157
MsaG046320 MtrunA17_Chr4g0004911 34.137 249 141 4 147 394 172 398 7.16e-38 144
MsaG046320 MtrunA17_Chr5g0447701 36.596 235 132 5 160 392 93 312 1.61e-37 142
MsaG046320 MtrunA17_Chr7g0249301 54.430 79 36 0 316 394 276 354 8.22e-26 108
MsaG046320 MtrunA17_Chr7g0249301 42.857 98 54 2 315 412 46 141 1.05e-15 79.0
MsaG046320 MtrunA17_Chr7g0249301 46.914 81 38 3 160 240 63 138 2.64e-12 68.6
MsaG046320 MtrunA17_Chr5g0447711 30.833 240 124 7 160 391 84 289 1.10e-23 101
MsaG046320 MtrunA17_Chr1g0150801 51.316 76 37 0 313 388 83 158 9.20e-22 92.0
MsaG046320 MtrunA17_Chr2g0294231 41.818 110 55 2 315 419 186 291 2.03e-21 95.9
MsaG046320 MtrunA17_Chr4g0051181 50.000 80 39 1 314 392 225 304 1.94e-20 94.0
MsaG046320 MtrunA17_Chr1g0188941 40.476 84 50 0 310 393 29 112 3.63e-20 85.9
MsaG046320 MtrunA17_Chr1g0194481 41.489 94 55 0 298 391 124 217 4.08e-20 91.3
MsaG046320 MtrunA17_Chr4g0051881 44.231 104 49 2 318 421 164 258 5.16e-20 91.3
MsaG046320 MtrunA17_Chr8g0335811 47.945 73 38 0 316 388 138 210 6.95e-20 88.6
MsaG046320 MtrunA17_Chr4g0060271 50.000 80 39 1 314 392 237 316 1.18e-19 92.0
MsaG046320 MtrunA17_Chr7g0270191 38.679 106 65 0 287 392 100 205 1.26e-19 89.4
MsaG046320 MtrunA17_Chr8g0383251 46.250 80 43 0 313 392 89 168 1.73e-19 86.3
MsaG046320 MtrunA17_Chr3g0129361 47.945 73 38 0 316 388 128 200 2.76e-19 86.7
MsaG046320 MtrunA17_Chr4g0053281 57.895 57 23 1 333 388 267 323 2.95e-19 89.0
MsaG046320 MtrunA17_Chr7g0270831 49.367 79 39 1 314 391 198 276 3.31e-19 89.7
MsaG046320 MtrunA17_Chr2g0302391 30.481 187 117 4 272 454 148 325 6.00e-19 89.0
MsaG046320 MtrunA17_Chr3g0099641 39.623 106 55 2 316 420 235 332 7.57e-19 89.0
MsaG046320 MtrunA17_Chr2g0288871 48.052 77 39 1 316 391 289 365 1.02e-18 89.0
MsaG046320 MtrunA17_Chr4g0075201 36.154 130 79 2 316 441 251 380 1.27e-18 88.6
MsaG046320 MtrunA17_Chr7g0216891 48.750 80 40 1 310 388 197 276 1.64e-18 88.6
MsaG046320 MtrunA17_Chr7g0224801 48.000 75 37 1 320 394 81 153 2.23e-18 82.8
MsaG046320 MtrunA17_Chr3g0129321 33.077 130 84 2 264 392 51 178 2.86e-18 83.2
MsaG046320 MtrunA17_Chr8g0386241 30.769 195 102 8 200 394 2 163 2.87e-18 82.8
MsaG046320 MtrunA17_Chr7g0238921 47.368 76 39 1 313 388 106 180 3.04e-18 85.5
MsaG046320 MtrunA17_Chr5g0401821 57.895 57 23 1 333 388 200 256 3.37e-18 84.7
MsaG046320 MtrunA17_Chr2g0322391 49.351 77 38 1 316 391 333 409 4.37e-18 87.4
MsaG046320 MtrunA17_Chr5g0418781 43.158 95 46 1 296 390 96 182 4.73e-18 84.3
MsaG046320 MtrunA17_Chr8g0383351 47.297 74 38 1 316 388 143 216 6.06e-18 83.2
MsaG046320 MtrunA17_Chr5g0443131 46.667 75 40 0 318 392 143 217 7.37e-18 84.3
MsaG046320 MtrunA17_Chr1g0147881 56.140 57 24 1 333 388 263 319 4.38e-17 82.4
MsaG046320 MtrunA17_Chr1g0184691 56.140 57 24 1 333 388 294 350 6.64e-17 82.0
MsaG046320 MtrunA17_Chr1g0202461 43.373 83 45 2 313 394 243 324 6.76e-17 82.4
MsaG046320 MtrunA17_Chr4g0068541 54.545 66 30 0 327 392 203 268 8.27e-17 82.4
MsaG046320 MtrunA17_Chr4g0025061 33.103 145 81 5 317 445 345 489 1.25e-16 82.8
MsaG046320 MtrunA17_Chr4g0071521 53.333 60 27 1 330 388 243 302 1.48e-16 80.5
MsaG046320 MtrunA17_Chr7g0228941 51.613 62 29 1 330 390 50 111 1.76e-16 75.9
MsaG046320 MtrunA17_Chr3g0136961 40.506 79 45 2 312 388 117 195 3.64e-16 79.0
MsaG046320 MtrunA17_Chr4g0001361 37.391 115 57 4 320 430 149 252 4.45e-16 79.3
MsaG046320 MtrunA17_Chr2g0319961 48.485 66 33 1 333 397 81 146 7.06e-16 77.8
MsaG046320 MtrunA17_Chr6g0485431 50.769 65 31 1 333 396 155 219 1.07e-15 78.6
MsaG046320 MtrunA17_Chr7g0248721 43.421 76 42 1 313 388 122 196 1.12e-15 76.3
MsaG046320 MtrunA17_Chr7g0270051 46.154 65 34 1 329 392 50 114 2.88e-15 75.9
MsaG046320 MtrunA17_Chr8g0379461 52.381 63 29 1 333 394 77 139 3.41e-15 75.1
MsaG046320 MtrunA17_Chr4g0025051 40.000 95 56 1 302 395 305 399 3.76e-15 78.2
MsaG046320 MtrunA17_Chr3g0139021 52.459 61 28 1 330 389 312 372 4.67e-15 77.0
MsaG046320 MtrunA17_Chr1g0150281 49.123 57 28 1 333 388 141 197 8.66e-15 74.7
MsaG046320 MtrunA17_Chr2g0333851 42.466 73 41 1 320 391 157 229 1.30e-14 74.7
MsaG046320 MtrunA17_Chr2g0289181 46.875 64 33 1 333 395 15 78 5.29e-14 71.6
MsaG046320 MtrunA17_Chr8g0348311 40.000 95 50 3 307 396 126 218 6.48e-14 72.8
MsaG046320 MtrunA17_Chr1g0150271 44.444 63 34 1 330 391 99 161 6.97e-14 71.6
MsaG046320 MtrunA17_Chr2g0284321 46.032 63 33 1 333 394 36 98 7.02e-14 70.9
MsaG046320 MtrunA17_Chr7g0248691 46.970 66 34 1 330 394 152 217 1.75e-13 71.6
MsaG046320 MtrunA17_Chr1g0189391 37.624 101 60 2 298 395 61 161 2.23e-13 71.2
MsaG046320 MtrunA17_Chr2g0293481 46.269 67 33 2 333 398 268 332 3.22e-13 71.6
MsaG046320 MtrunA17_Chr8g0387501 38.000 100 49 4 329 425 126 215 6.32e-13 68.9
MsaG046320 MtrunA17_Chr5g0432071 36.486 74 46 1 333 406 105 177 2.06e-11 63.5
MsaG046320 MtrunA17_Chr5g0432071 44.737 76 41 1 143 218 88 162 3.77e-11 62.8
MsaG046320 MtrunA17_Chr5g0419241 55.556 45 20 0 316 360 61 105 4.29e-11 60.1
MsaG046320 MtrunA17_Chr4g0024391 33.333 93 59 2 302 393 199 289 4.69e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046320 AT4G30935.1 48.760 484 198 14 13 490 27 466 3.08e-125 373
MsaG046320 AT2G03340.1 34.000 350 192 10 64 394 148 477 2.54e-50 179
MsaG046320 AT1G13960.2 33.837 331 191 9 73 391 1 315 6.16e-48 169
MsaG046320 AT4G26640.1 36.149 296 154 6 113 394 97 371 7.15e-48 172
MsaG046320 AT4G26640.1 52.239 67 31 1 330 396 137 202 9.92e-15 77.0
MsaG046320 AT4G26640.5 36.525 282 148 5 127 394 3 267 7.69e-48 170
MsaG046320 AT4G26640.5 52.239 67 31 1 330 396 33 98 1.25e-14 75.9
MsaG046320 AT4G26640.6 36.525 282 148 5 127 394 3 267 7.69e-48 170
MsaG046320 AT4G26640.6 52.239 67 31 1 330 396 33 98 1.25e-14 75.9
MsaG046320 AT4G26640.4 36.525 282 148 5 127 394 3 267 7.69e-48 170
MsaG046320 AT4G26640.4 52.239 67 31 1 330 396 33 98 1.25e-14 75.9
MsaG046320 AT4G26640.3 36.525 282 148 5 127 394 3 267 7.69e-48 170
MsaG046320 AT4G26640.3 52.239 67 31 1 330 396 33 98 1.25e-14 75.9
MsaG046320 AT4G26640.7 36.149 296 154 6 113 394 129 403 1.17e-47 172
MsaG046320 AT4G26640.7 52.239 67 31 1 330 396 169 234 9.38e-15 77.0
MsaG046320 AT1G13960.1 31.865 386 228 10 14 391 110 468 1.40e-47 172
MsaG046320 AT4G26640.2 36.149 296 154 6 113 394 169 443 1.70e-47 173
MsaG046320 AT4G26640.2 52.239 67 31 1 330 396 209 274 1.00e-14 77.0
MsaG046320 AT2G38470.1 38.583 254 123 5 160 394 185 424 3.97e-45 166
MsaG046320 AT2G38470.1 42.683 82 46 1 333 414 185 265 3.51e-13 72.0
MsaG046320 AT4G26440.1 38.202 267 128 8 157 394 176 434 6.17e-45 166
MsaG046320 AT4G26440.1 45.714 70 37 1 333 402 179 247 3.91e-13 72.0
MsaG046320 AT5G07100.2 41.152 243 83 9 158 399 23 206 1.54e-43 153
MsaG046320 AT5G07100.4 41.152 243 83 9 158 399 23 206 1.54e-43 153
MsaG046320 AT5G07100.5 41.152 243 83 9 158 399 23 206 1.54e-43 153
MsaG046320 AT5G07100.3 41.152 243 83 9 158 399 23 206 1.54e-43 153
MsaG046320 AT5G07100.1 41.152 243 83 9 158 399 116 299 1.58e-43 156
MsaG046320 AT2G04880.2 35.650 331 165 11 94 391 27 342 2.62e-43 159
MsaG046320 AT2G04880.1 33.815 346 175 11 94 391 27 366 3.23e-40 151
MsaG046320 AT2G04880.1 50.000 60 29 1 333 392 112 170 2.80e-12 69.3
MsaG046320 AT3G01080.1 35.443 237 115 5 157 391 165 365 4.59e-36 139
MsaG046320 AT3G01080.1 46.753 77 40 1 333 409 168 243 1.35e-14 76.3
MsaG046320 AT3G01080.2 35.443 237 115 5 157 391 203 403 4.73e-36 139
MsaG046320 AT3G01080.2 46.753 77 40 1 333 409 206 281 1.24e-14 76.6
MsaG046320 AT2G30250.1 35.124 242 140 4 158 396 165 392 1.14e-35 137
MsaG046320 AT2G30250.1 49.315 73 36 1 331 403 165 236 3.43e-12 68.6
MsaG046320 AT2G37260.3 37.743 257 119 9 158 388 84 325 1.02e-33 130
MsaG046320 AT2G37260.2 37.743 257 119 9 158 388 82 323 1.03e-33 130
MsaG046320 AT2G37260.1 37.743 257 119 9 158 388 164 405 1.57e-33 132
MsaG046320 AT5G56270.1 51.020 98 48 0 297 394 452 549 1.27e-26 114
MsaG046320 AT5G56270.1 51.515 66 31 1 333 398 274 338 3.95e-15 78.6
MsaG046320 AT5G56270.1 32.800 125 81 2 103 225 217 340 2.11e-12 70.1
MsaG046320 AT4G12020.3 34.328 201 118 5 159 352 468 661 6.06e-24 106
MsaG046320 AT4G12020.3 49.153 59 29 1 333 391 469 526 6.16e-12 68.9
MsaG046320 AT4G12020.10 34.328 201 118 5 159 352 468 661 6.75e-24 106
MsaG046320 AT4G12020.10 49.153 59 29 1 333 391 469 526 6.42e-12 68.9
MsaG046320 AT4G12020.2 34.328 201 118 5 159 352 468 661 6.77e-24 106
MsaG046320 AT4G12020.2 49.153 59 29 1 333 391 469 526 6.55e-12 68.9
MsaG046320 AT4G12020.4 34.328 201 118 5 159 352 468 661 7.14e-24 106
MsaG046320 AT4G12020.4 49.153 59 29 1 333 391 469 526 6.72e-12 68.9
MsaG046320 AT4G12020.7 34.328 201 118 5 159 352 468 661 7.64e-24 106
MsaG046320 AT4G12020.7 49.153 59 29 1 333 391 469 526 6.05e-12 68.9
MsaG046320 AT4G12020.6 34.328 201 118 5 159 352 468 661 7.68e-24 106
MsaG046320 AT4G12020.6 49.153 59 29 1 333 391 469 526 6.15e-12 68.9
MsaG046320 AT4G12020.8 34.328 201 118 5 159 352 468 661 7.68e-24 106
MsaG046320 AT4G12020.8 49.153 59 29 1 333 391 469 526 6.15e-12 68.9
MsaG046320 AT4G12020.1 34.328 201 118 5 159 352 468 661 7.72e-24 106
MsaG046320 AT4G12020.1 49.153 59 29 1 333 391 469 526 6.22e-12 68.9
MsaG046320 AT4G12020.9 34.328 201 118 5 159 352 468 661 7.72e-24 106
MsaG046320 AT4G12020.9 49.153 59 29 1 333 391 469 526 6.22e-12 68.9
MsaG046320 AT4G12020.5 34.328 201 118 5 159 352 401 594 8.86e-24 106
MsaG046320 AT4G12020.5 49.153 59 29 1 333 391 402 459 7.28e-12 68.9
MsaG046320 AT4G12020.11 34.328 201 118 5 159 352 468 661 1.81e-23 105
MsaG046320 AT4G12020.11 49.153 59 29 1 333 391 469 526 9.72e-12 68.2
MsaG046320 AT1G55600.1 45.679 81 44 0 311 391 286 366 1.69e-20 94.7
MsaG046320 AT5G49520.1 42.105 95 54 1 316 409 205 299 2.37e-20 93.6
MsaG046320 AT5G41570.1 47.368 76 40 0 313 388 79 154 2.80e-20 88.6
MsaG046320 AT1G69310.4 43.878 98 49 1 316 413 131 222 3.66e-20 91.3
MsaG046320 AT1G69310.2 43.878 98 49 1 316 413 131 222 3.66e-20 91.3
MsaG046320 AT1G69310.1 43.878 98 49 1 316 413 131 222 3.66e-20 91.3
MsaG046320 AT5G26170.1 54.839 62 28 0 327 388 108 169 1.21e-19 86.7
MsaG046320 AT2G23320.1 61.404 57 21 1 333 388 241 297 1.45e-19 89.7
MsaG046320 AT2G44745.1 43.023 86 49 0 303 388 116 201 1.78e-19 87.4
MsaG046320 AT5G13080.1 47.368 76 38 1 319 394 54 127 2.03e-19 85.1
MsaG046320 AT4G04450.2 43.689 103 49 3 316 417 182 276 3.02e-19 90.5
MsaG046320 AT5G15130.2 39.823 113 63 2 316 427 169 277 4.32e-19 90.5
MsaG046320 AT5G15130.1 39.823 113 63 2 316 427 211 319 4.70e-19 90.5
MsaG046320 AT1G68150.1 47.619 84 43 1 310 392 213 296 4.76e-19 89.4
MsaG046320 AT2G24570.1 50.000 78 38 1 333 409 244 321 5.02e-19 88.6
MsaG046320 AT1G64000.1 48.571 70 36 0 319 388 101 170 5.02e-19 85.5
MsaG046320 AT4G04450.1 43.689 103 49 3 316 417 276 370 7.58e-19 89.7
MsaG046320 AT4G18170.1 44.828 87 46 1 312 398 152 236 9.49e-19 87.4
MsaG046320 AT4G22070.4 49.351 77 38 1 316 391 281 357 1.05e-18 89.4
MsaG046320 AT4G22070.3 49.351 77 38 1 316 391 281 357 1.05e-18 89.4
MsaG046320 AT4G22070.2 49.351 77 38 1 316 391 281 357 1.05e-18 89.4
MsaG046320 AT4G22070.1 49.351 77 38 1 316 391 281 357 1.05e-18 89.4
MsaG046320 AT3G01970.1 48.571 70 36 0 319 388 52 121 1.28e-18 83.2
MsaG046320 AT4G24240.1 56.667 60 25 1 330 388 279 338 1.48e-18 87.4
MsaG046320 AT4G39410.1 49.315 73 37 0 316 388 207 279 1.65e-18 86.7
MsaG046320 AT4G01720.1 49.367 79 39 1 314 391 221 299 3.74e-18 87.4
MsaG046320 AT1G62300.1 49.367 79 37 2 316 393 296 372 4.92e-18 87.4
MsaG046320 AT3G04670.1 57.895 57 23 1 333 388 263 319 5.07e-18 85.5
MsaG046320 AT4G31550.1 58.621 58 23 1 333 389 247 304 5.97e-18 85.1
MsaG046320 AT4G31550.2 58.621 58 23 1 333 389 246 303 6.07e-18 85.1
MsaG046320 AT5G46350.1 38.739 111 55 4 333 440 184 284 1.03e-17 84.7
MsaG046320 AT5G28650.1 57.895 57 23 1 333 388 263 319 1.12e-17 84.7
MsaG046320 AT5G43290.1 51.613 62 30 0 329 390 111 172 1.18e-17 83.6
MsaG046320 AT2G47260.1 52.381 63 30 0 333 395 175 237 1.28e-17 84.3
MsaG046320 AT5G64810.1 49.206 63 32 0 328 390 106 168 2.08e-17 80.9
MsaG046320 AT1G29860.1 46.988 83 40 2 333 413 137 217 2.13e-17 82.8
MsaG046320 AT2G46130.1 46.053 76 41 0 313 388 11 86 2.16e-17 78.2
MsaG046320 AT3G62340.1 36.879 141 74 3 315 452 101 229 1.34e-16 80.5
MsaG046320 AT1G29280.1 48.529 68 34 1 326 392 68 135 6.36e-16 78.2
MsaG046320 AT1G18860.1 46.835 79 39 2 316 393 223 299 1.21e-15 79.7
MsaG046320 AT2G30590.1 52.632 57 26 1 333 388 314 370 1.53e-15 78.6
MsaG046320 AT1G69310.3 40.816 98 48 2 316 413 131 218 2.23e-15 77.0
MsaG046320 AT2G21900.1 49.153 59 30 0 333 391 110 168 3.93e-15 74.7
MsaG046320 AT3G01080.3 46.753 77 40 1 333 409 206 281 6.32e-15 76.3
MsaG046320 AT4G31800.2 45.000 60 32 1 333 391 112 171 1.54e-14 73.9
MsaG046320 AT4G23550.1 29.814 161 86 3 235 394 69 203 1.61e-14 74.7
MsaG046320 AT5G52830.1 50.000 64 31 1 330 392 163 226 1.84e-14 75.1
MsaG046320 AT4G31800.1 45.000 60 32 1 333 391 177 236 1.92e-14 74.7
MsaG046320 AT4G01250.1 45.000 80 40 3 319 397 116 192 5.40e-14 73.2
MsaG046320 AT2G25000.2 45.000 60 32 1 333 391 104 163 6.35e-14 71.6
MsaG046320 AT2G25000.4 45.000 60 32 1 333 391 104 163 6.35e-14 71.6
MsaG046320 AT1G80840.1 38.356 73 44 1 320 391 134 206 7.38e-14 72.8
MsaG046320 AT1G69810.2 45.570 79 41 2 316 392 129 207 1.01e-13 72.8
MsaG046320 AT1G69810.1 45.570 79 41 2 316 392 187 265 1.06e-13 73.2
MsaG046320 AT2G25000.3 45.000 60 32 1 333 391 126 185 1.29e-13 71.2
MsaG046320 AT2G25000.1 45.000 60 32 1 333 391 147 206 1.35e-13 71.6
MsaG046320 AT3G58710.1 47.761 67 34 1 326 391 65 131 1.77e-13 71.2
MsaG046320 AT2G34830.1 43.421 76 39 3 324 398 208 280 1.80e-13 72.8
MsaG046320 AT3G58710.2 47.761 67 34 1 326 391 64 130 1.87e-13 71.2
MsaG046320 AT2G34830.2 43.421 76 39 3 324 398 217 289 1.95e-13 72.8
MsaG046320 AT1G30650.1 43.056 72 39 2 324 394 210 280 1.11e-12 70.1
MsaG046320 AT2G46130.2 42.105 76 40 1 313 388 11 82 1.45e-12 64.3
MsaG046320 AT5G45050.4 45.588 68 34 2 331 397 1040 1105 2.78e-11 66.6
MsaG046320 AT5G45050.1 45.588 68 34 2 331 397 1179 1244 2.93e-11 66.6
MsaG046320 AT5G45050.2 45.588 68 34 2 331 397 1151 1216 3.13e-11 66.6

Find 127 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACCGAATTCAGCGTATT+AGG 0.178386 8:+83299126 None:intergenic
GGATTCAACTGGCGGAAATA+TGG 0.214937 8:-83297758 MsaT046320.1:CDS
GGTTGTCAAATCCGAAATTA+AGG 0.234424 8:-83299109 MsaT046320.1:intron
ACGCGGACTTGCCTTCGTTT+TGG 0.267382 8:+83297353 None:intergenic
CAACCGAATTCAGCGTATTA+GGG 0.297461 8:+83299127 None:intergenic
AAATTATCTAACCTTAATTT+CGG 0.308223 8:+83299098 None:intergenic
CCGGGGAAGCCAAGGACCTT+GGG 0.325495 8:-83294952 MsaT046320.1:CDS
CGGCCTGAGCTGAAGGTTCC+TGG 0.331309 8:+83298002 None:intergenic
GGATACCGATGGCGCAAGTA+TGG 0.334207 8:-83297123 MsaT046320.1:CDS
AGGTGTTTCTTCTTGAGCTT+TGG 0.353643 8:+83297466 None:intergenic
CGCAAACTTACTTTCCCTAA+TGG 0.358180 8:+83297571 None:intergenic
GCGCAAGTATGGACAGAAAA+TGG 0.361245 8:-83297112 MsaT046320.1:CDS
ACTCAACTGCAAATTATTCA+TGG 0.361645 8:+83295042 None:intergenic
GCAGGAGATGTGGGGATATC+TGG 0.371320 8:-83297150 MsaT046320.1:CDS
CAGGTTCTCGAAGCTATTAC+AGG 0.382750 8:-83297709 MsaT046320.1:CDS
ACGGAAGATTAGTTCCATTA+GGG 0.383627 8:-83297585 MsaT046320.1:CDS
ATTGAATGCTGGGATCATTC+AGG 0.394765 8:-83297656 MsaT046320.1:CDS
AGACATATTCTGTATTTGTA+TGG 0.407890 8:+83297886 None:intergenic
CACGGAAGATTAGTTCCATT+AGG 0.408040 8:-83297586 MsaT046320.1:CDS
TGCTGCATGTACAACAAACT+TGG 0.409694 8:+83297170 None:intergenic
CCATCAGATGATGGTGTCCT+TGG 0.410812 8:+83297779 None:intergenic
CCCAAGGTCCTTGGCTTCCC+CGG 0.414124 8:+83294952 None:intergenic
GGTGGGCCGTGTCGTTTCTT+CGG 0.415960 8:+83295160 None:intergenic
TTCTTGAGCTTTGGAAGATG+TGG 0.417147 8:+83297475 None:intergenic
GTCTCAATGTGCTTTCGAAC+AGG 0.417742 8:+83295250 None:intergenic
TGGGCACAGCAAATTATTCA+TGG 0.422354 8:+83295102 None:intergenic
TGTCTTCTGTTTCTGTTCCA+AGG 0.425869 8:-83297796 MsaT046320.1:CDS
ACCGGGGAAGCCAAGGACCT+TGG 0.430810 8:-83294953 MsaT046320.1:CDS
AATCCCTAATACGCTGAATT+CGG 0.433253 8:-83299130 MsaT046320.1:CDS
CTCTGCAGTCGGTTCGTTGG+AGG 0.433498 8:+83297547 None:intergenic
ATCAGAAAATTTCTACTCAA+TGG 0.437791 8:-83295000 MsaT046320.1:CDS
GGTGCTGCAGCAGCCACAAG+AGG 0.441768 8:+83295130 None:intergenic
ATATTCTGTATTTGTATGGT+TGG 0.451171 8:+83297890 None:intergenic
TTCCCCGGTTAGTTCTCCTT+CGG 0.452841 8:+83294967 None:intergenic
TACTACAGATGCACTTCTGC+TGG 0.452929 8:-83295277 MsaT046320.1:CDS
GTGGATGGATCAGAATCTTT+CGG 0.455352 8:+83297494 None:intergenic
CCGTGTCGTTTCTTCGGCAC+AGG 0.460742 8:+83295166 None:intergenic
TGCTAAAAGAGAAGCTGTCC+AGG 0.461739 8:-83298020 MsaT046320.1:CDS
GATTCAACTGGCGGAAATAT+GGG 0.464037 8:-83297757 MsaT046320.1:CDS
TCTGTCCATACTTGCGCCAT+CGG 0.468804 8:+83297118 None:intergenic
AGAAGCTGAGTGGGTCCCAC+AGG 0.472843 8:+83299414 None:intergenic
TGCTCTTCGGCCTGAGCTGA+AGG 0.475117 8:+83297995 None:intergenic
TAATTTGCAGTTGAGTAAAC+CGG 0.477622 8:-83295035 MsaT046320.1:CDS
GTTCTCTGCAGTCGGTTCGT+TGG 0.479965 8:+83297544 None:intergenic
GAATCTTTCGGAGCATTGAC+AGG 0.481035 8:+83297506 None:intergenic
TGTATTTGTATGGTTGGACT+AGG 0.483789 8:+83297896 None:intergenic
TCATCTGATATCAATGGTAA+TGG 0.485405 8:-83297416 MsaT046320.1:CDS
GACACCGAAGGAGAACTAAC+CGG 0.487157 8:-83294971 MsaT046320.1:CDS
GCTGACGGTGATTCGCCGAC+TGG 0.493066 8:+83299354 None:intergenic
TGTCCAGGAACCTTCAGCTC+AGG 0.494745 8:-83298005 MsaT046320.1:CDS
AAAGACCTTTACCTGGGATG+TGG 0.496114 8:+83297078 None:intergenic
TCTGCAGTCGGTTCGTTGGA+GGG 0.497315 8:+83297548 None:intergenic
TGCTAAGAAGATTGAATGCT+GGG 0.499049 8:-83297666 MsaT046320.1:CDS
CATCATCTGATGGATTCAAC+TGG 0.499437 8:-83297769 MsaT046320.1:CDS
CCCACCAAGTGCTCCTCTTG+TGG 0.499953 8:-83295083 MsaT046320.1:CDS
TGCTGTCATCATAACTTACA+AGG 0.506337 8:-83295209 MsaT046320.1:CDS
AAGTTTGTTGTACATGCAGC+AGG 0.506685 8:-83297168 MsaT046320.1:CDS
GTCTCCGAAGCTCTCCCTGT+GGG 0.511729 8:-83299429 MsaT046320.1:CDS
CAGAAAGGAGAAAGGTGACT+TGG 0.514032 8:-83297229 MsaT046320.1:CDS
CCCACCAAGTGCTCCTCTTG+TGG 0.523692 8:-83295143 MsaT046320.1:CDS
ACACCGAAGGAGAACTAACC+GGG 0.523728 8:-83294970 MsaT046320.1:CDS
GGGTCCCACAGGGAGAGCTT+CGG 0.524421 8:+83299425 None:intergenic
CAAAACTCATCTGATATCAA+TGG 0.525848 8:-83297422 MsaT046320.1:CDS
TGGACCTCTTGAAAATGAAA+AGG 0.527268 8:-83298047 MsaT046320.1:CDS
TGCAAATTATTCATGGAGGC+AGG 0.533719 8:+83295049 None:intergenic
AGCAAATTATTCATGGAGGC+AGG 0.536158 8:+83295109 None:intergenic
GCCACAAGAGGAGCACTTGG+TGG 0.539995 8:+83295142 None:intergenic
GCTCGAACGCTTCTGAGCAT+AGG 0.544980 8:-83294908 MsaT046320.1:CDS
TTTGGTGATAGTTCAGATGG+TGG 0.547468 8:+83297947 None:intergenic
TTTCATGCTCAGGAAACGGT+TGG 0.552316 8:-83298067 MsaT046320.1:intron
TTCTGCGACTCGGTGGCTGA+CGG 0.553411 8:+83299339 None:intergenic
GAATTTGGTGATAGTTCAGA+TGG 0.554364 8:+83297944 None:intergenic
AGAACTAACCGGGGAAGCCA+AGG 0.555363 8:-83294960 MsaT046320.1:CDS
GCCACAAGAGGAGCACTTGG+TGG 0.560730 8:+83295082 None:intergenic
TAATGGAACTAATCTTCCGT+GGG 0.563190 8:+83297588 None:intergenic
TAGCTTCGAGAACCTGTAGT+AGG 0.565059 8:+83297716 None:intergenic
CGGGGAAGCCAAGGACCTTG+GGG 0.569355 8:-83294951 MsaT046320.1:CDS
GCAGCCACAAGAGGAGCACT+TGG 0.569920 8:+83295139 None:intergenic
CCACAAGAGGAGCACTTGGT+GGG 0.571996 8:+83295083 None:intergenic
GACAGCATCTGAGTTGTCTA+CGG 0.572444 8:+83295225 None:intergenic
CCAAGGACACCATCATCTGA+TGG 0.573655 8:-83297779 MsaT046320.1:CDS
CAGTTGAATCCATCAGATGA+TGG 0.574887 8:+83297770 None:intergenic
CAAGTTAAGAGTCCTACTAC+AGG 0.575076 8:-83297728 MsaT046320.1:CDS
AAGACCTTTACCTGGGATGT+GGG 0.577183 8:+83297079 None:intergenic
TGTTCTGGGAGAAGCTGAGT+GGG 0.577794 8:+83299405 None:intergenic
GTTGATTTCACAGATGTGCA+AGG 0.578017 8:-83299153 MsaT046320.1:intron
TGAGCTTTGGAAGATGTGGA+TGG 0.579447 8:+83297479 None:intergenic
GCTGCATGTACAACAAACTT+GGG 0.581848 8:+83297171 None:intergenic
TTGATAACCAGAAAGGAGAA+AGG 0.582120 8:-83297237 MsaT046320.1:intron
TTGTATGGTTGGACTAGGAA+CGG 0.583444 8:+83297901 None:intergenic
CCACAAGAGGAGCACTTGGT+GGG 0.590936 8:+83295143 None:intergenic
AGTCACTTGAAGCTGCTCTT+CGG 0.591198 8:+83297982 None:intergenic
GGTGCTGCATCAGCCACAAG+AGG 0.591740 8:+83295070 None:intergenic
GTGCTAAGAAGATTGAATGC+TGG 0.592242 8:-83297667 MsaT046320.1:CDS
CTAATGGAACTAATCTTCCG+TGG 0.592547 8:+83297587 None:intergenic
GGAGACGCGATGCTCAGCCA+TGG 0.594531 8:+83299446 None:intergenic
GGCGTATTACTAACGATTGG+AGG 0.596429 8:+83297863 None:intergenic
GATGTGGGGATATCTGGTGA+TGG 0.598874 8:-83297144 MsaT046320.1:CDS
TCAGCCACAAGAGGAGCACT+TGG 0.605078 8:+83295079 None:intergenic
GTACATGCAGCAGGAGATGT+GGG 0.608887 8:-83297159 MsaT046320.1:CDS
TGAAGGGTAACCCACATCCC+AGG 0.609054 8:-83297089 MsaT046320.1:intron
ACAGGCGTATTACTAACGAT+TGG 0.613651 8:+83297860 None:intergenic
AAAACACTGTTCTCTGCAGT+CGG 0.615994 8:+83297536 None:intergenic
GTATGGACAGAAAATGGTGA+AGG 0.618010 8:-83297106 MsaT046320.1:CDS
CATCTGATGGATTCAACTGG+CGG 0.620518 8:-83297766 MsaT046320.1:CDS
TATGGACAGAAAATGGTGAA+GGG 0.621435 8:-83297105 MsaT046320.1:CDS
GGTTTCTTGTCAGAACTGCA+AGG 0.624573 8:+83297446 None:intergenic
TCTGAATGATGAATTCAGCA+AGG 0.627601 8:+83294872 None:intergenic
CGTCTCCGAAGCTCTCCCTG+TGG 0.630117 8:-83299430 MsaT046320.1:CDS
GGGGAAGCCAAGGACCTTGG+GGG 0.641628 8:-83294950 MsaT046320.1:CDS
TGGAACAGAAACAGAAGACA+AGG 0.643346 8:+83297799 None:intergenic
AATTTCTACTCAATGGTCAG+TGG 0.645416 8:-83294993 MsaT046320.1:CDS
TTGTTCTGGGAGAAGCTGAG+TGG 0.645709 8:+83299404 None:intergenic
CAATGGTCAGTGGACACCGA+AGG 0.651277 8:-83294983 MsaT046320.1:CDS
TGTACATGCAGCAGGAGATG+TGG 0.652491 8:-83297160 MsaT046320.1:CDS
GAAGCTGAGTGGGTCCCACA+GGG 0.663562 8:+83299415 None:intergenic
GCTGTCATCATAACTTACAA+GGG 0.670071 8:-83295208 MsaT046320.1:CDS
AATCGTTAGTAATACGCCTG+TGG 0.690746 8:-83297858 MsaT046320.1:CDS
TACATGCAGCAGGAGATGTG+GGG 0.691459 8:-83297158 MsaT046320.1:CDS
ACGAGCCAGATCCAAAACGA+AGG 0.695030 8:-83297364 MsaT046320.1:intron
TATCTGGTGATGGATACCGA+TGG 0.698652 8:-83297134 MsaT046320.1:CDS
CCTGTGCCGAAGAAACGACA+CGG 0.708728 8:-83295166 MsaT046320.1:CDS
ATGGAACTAATCTTCCGTGG+GGG 0.722546 8:+83297590 None:intergenic
CAACTGCAAATTATTCATGG+AGG 0.726603 8:+83295045 None:intergenic
AATGGAACTAATCTTCCGTG+GGG 0.732859 8:+83297589 None:intergenic
CACCGAAGGAGAACTAACCG+GGG 0.738224 8:-83294969 MsaT046320.1:CDS
GCACAGCAAATTATTCATGG+AGG 0.739037 8:+83295105 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 83294886 83299466 83294886 ID=MsaG046320
Chr8 mRNA 83294886 83299466 83294886 ID=MsaT046320.1;Parent=MsaG046320
Chr8 exon 83294886 83295303 83294886 ID=MsaT046320.1.exon5;Parent=MsaT046320.1
Chr8 CDS 83294886 83295303 83294886 ID=cds.MsaT046320.1;Parent=MsaT046320.1
Chr8 exon 83297090 83297248 83297090 ID=MsaT046320.1.exon4;Parent=MsaT046320.1
Chr8 CDS 83297090 83297248 83297090 ID=cds.MsaT046320.1;Parent=MsaT046320.1
Chr8 exon 83297365 83298077 83297365 ID=MsaT046320.1.exon3;Parent=MsaT046320.1
Chr8 CDS 83297365 83298077 83297365 ID=cds.MsaT046320.1;Parent=MsaT046320.1
Chr8 exon 83299110 83299162 83299110 ID=MsaT046320.1.exon2;Parent=MsaT046320.1
Chr8 CDS 83299110 83299162 83299110 ID=cds.MsaT046320.1;Parent=MsaT046320.1
Chr8 exon 83299337 83299466 83299337 ID=MsaT046320.1.exon1;Parent=MsaT046320.1
Chr8 CDS 83299337 83299466 83299337 ID=cds.MsaT046320.1;Parent=MsaT046320.1
Gene Sequence

>MsaT046320.1

ATGGCTGAGCATCGCGTCTCCGAAGCTCTCCCTGTGGGACCCACTCAGCTTCTCCCAGAACAACAAAAAGCTCAAGAGATAAAAAAGGAACCAGTCGGCGAATCACCGTCAGCCACCGAGTCGCAGAAAAATGTGCAAGGAATCCCTAATACGCTGAATTCGGTTGTCAAATCCGAAATTAAGGAAACGGTTGGACCTCTTGAAAATGAAAAGGAAGTTGCTAAAAGAGAAGCTGTCCAGGAACCTTCAGCTCAGGCCGAAGAGCAGCTTCAAGTGACTGTGTCTTCAAATCCACCATCTGAACTATCACCAAATTCTGTTACAAAATCTTTATCATCCGTTCCTAGTCCAACCATACAAATACAGAATATGTCTCCTCCAATCGTTAGTAATACGCCTGTGGCAGAAGTAAACAAAAAAAGTACTTCTGGCGGTAGAGCCTTGTCTTCTGTTTCTGTTCCAAGGACACCATCATCTGATGGATTCAACTGGCGGAAATATGGGCAGAAGCAAGTTAAGAGTCCTACTACAGGTTCTCGAAGCTATTACAGGTGTACTCAATCAAACTGTTGTGCTAAGAAGATTGAATGCTGGGATCATTCAGGTCATGTGATAGAGACTGTTTATAAAAGTGAGCACAGTCATGATCCCCCACGGAAGATTAGTTCCATTAGGGAAAGTAAGTTTGCGCCCTCCAACGAACCGACTGCAGAGAACAGTGTTTTAGTGAAGCCTGTCAATGCTCCGAAAGATTCTGATCCATCCACATCTTCCAAAGCTCAAGAAGAAACACCTTGCAGTTCTGACAAGAAACCACAAAACTCATCTGATATCAATGGTAATGGTAAAATAGTTTTGAACGAGGAACATGTCGACGAGCCAGATCCAAAACGAAGAAAGGAGAAAGGTGACTTGGTTCACTCAGATTCTTCTGTAAAACCTGAAAAGAAACCCAAGTTTGTTGTACATGCAGCAGGAGATGTGGGGATATCTGGTGATGGATACCGATGGCGCAAGTATGGACAGAAAATGGTGAAGGGTAACCCACATCCCAGAAACTACTACAGATGCACTTCTGCTGGATGTCCTGTTCGAAAGCACATTGAGACTGCCGTAGACAACTCAGATGCTGTCATCATAACTTACAAGGGTGTACATGATCATGACACTCCTGTGCCGAAGAAACGACACGGCCCACCAAGTGCTCCTCTTGTGGCTGCTGCAGCACCTGCCTCCATGAATAATTTGCTGTGCCCACCAAGTGCTCCTCTTGTGGCTGATGCAGCACCTGCCTCCATGAATAATTTGCAGTTGAGTAAACCGGACTCGCCACAAAATCAGAAAATTTCTACTCAATGGTCAGTGGACACCGAAGGAGAACTAACCGGGGAAGCCAAGGACCTTGGGGGTGAGAAAGCAATAGAGTCAGCTCGAACGCTTCTGAGCATAGGTTTTGAAATCAAGCCTTGCTGA
Protein sequence

>MsaT046320.1

MAEHRVSEALPVGPTQLLPEQQKAQEIKKEPVGESPSATESQKNVQGIPNTLNSVVKSEIKETVGPLENEKEVAKREAVQEPSAQAEEQLQVTVSSNPPSELSPNSVTKSLSSVPSPTIQIQNMSPPIVSNTPVAEVNKKSTSGGRALSSVSVPRTPSSDGFNWRKYGQKQVKSPTTGSRSYYRCTQSNCCAKKIECWDHSGHVIETVYKSEHSHDPPRKISSIRESKFAPSNEPTAENSVLVKPVNAPKDSDPSTSSKAQEETPCSSDKKPQNSSDINGNGKIVLNEEHVDEPDPKRRKEKGDLVHSDSSVKPEKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIETAVDNSDAVIITYKGVHDHDTPVPKKRHGPPSAPLVAAAAPASMNNLLCPPSAPLVADAAPASMNNLQLSKPDSPQNQKISTQWSVDTEGELTGEAKDLGGEKAIESARTLLSIGFEIKPC*