AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046335

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046335 MtrunA17_Chr4g0074161 97.022 403 4 3 1 395 8 410 0.0 783
MsaG046335 MtrunA17_Chr1g0190731 62.366 186 60 4 176 359 176 353 8.68e-62 202
MsaG046335 MtrunA17_Chr1g0184841 65.854 164 46 3 179 341 108 262 2.91e-54 181
MsaG046335 MtrunA17_Chr3g0131771 60.000 85 29 2 191 274 147 227 2.58e-23 99.8
MsaG046335 MtrunA17_Chr7g0234761 54.054 111 42 4 191 298 317 421 7.08e-23 100
MsaG046335 MtrunA17_Chr8g0339091 56.522 92 36 2 168 255 265 356 7.55e-23 100
MsaG046335 MtrunA17_Chr3g0144641 72.727 66 17 1 191 255 191 256 1.09e-22 99.0
MsaG046335 MtrunA17_Chr1g0152131 60.241 83 32 1 174 255 243 325 1.42e-22 98.6
MsaG046335 MtrunA17_Chr8g0365941 64.935 77 26 1 180 255 294 370 6.90e-22 97.1
MsaG046335 MtrunA17_Chr4g0035101 71.212 66 18 1 191 255 143 208 7.36e-22 95.5
MsaG046335 MtrunA17_Chr5g0421111 65.753 73 24 1 184 255 358 430 1.77e-21 96.3
MsaG046335 MtrunA17_Chr8g0385911 57.831 83 30 2 191 272 145 223 1.83e-21 94.4
MsaG046335 MtrunA17_Chr8g0363921 68.182 66 20 1 191 255 151 216 2.13e-21 93.2
MsaG046335 MtrunA17_Chr5g0415291 66.667 66 21 1 191 255 112 177 1.87e-20 89.7
MsaG046335 MtrunA17_Chr1g0193321 55.405 74 29 1 193 266 464 533 2.62e-19 90.1
MsaG046335 MtrunA17_Chr1g0153241 45.545 101 52 2 159 259 55 152 2.86e-19 87.0
MsaG046335 MtrunA17_Chr1g0152651 52.564 78 37 0 177 254 260 337 3.51e-19 88.2
MsaG046335 MtrunA17_Chr5g0393721 60.000 65 26 0 190 254 223 287 4.39e-19 87.4
MsaG046335 MtrunA17_Chr4g0014771 62.121 66 24 1 190 254 117 182 5.30e-19 84.3
MsaG046335 MtrunA17_Chr1g0182791 37.415 147 74 3 128 264 248 386 6.13e-19 88.6
MsaG046335 MtrunA17_Chr3g0134741 52.632 76 36 0 190 265 228 303 9.50e-19 86.3
MsaG046335 MtrunA17_Chr1g0174271 60.870 69 26 1 190 257 297 365 1.14e-18 87.0
MsaG046335 MtrunA17_Chr1g0174161 49.485 97 39 3 187 277 156 248 2.43e-18 85.1
MsaG046335 MtrunA17_Chr7g0258161 61.429 70 26 1 187 255 169 238 8.56e-18 84.0
MsaG046335 MtrunA17_Chr7g0262691 52.857 70 33 0 194 263 255 324 8.83e-18 84.7
MsaG046335 MtrunA17_Chr1g0207301 43.750 96 50 1 159 254 260 351 1.49e-17 83.6
MsaG046335 MtrunA17_Chr3g0095391 57.143 63 27 0 193 255 311 373 1.50e-17 84.3
MsaG046335 MtrunA17_Chr6g0484271 62.121 66 24 1 191 255 155 220 1.64e-17 82.0
MsaG046335 MtrunA17_Chr5g0435021 47.674 86 40 2 170 254 200 281 3.08e-17 81.6
MsaG046335 MtrunA17_Chr1g0207311 41.667 96 56 0 170 265 175 270 5.86e-17 80.5
MsaG046335 MtrunA17_Chr5g0402031 64.815 54 19 0 202 255 116 169 1.13e-16 80.5
MsaG046335 MtrunA17_Chr4g0032951 56.522 69 29 1 190 257 354 422 1.70e-16 80.9
MsaG046335 MtrunA17_Chr7g0271251 52.632 76 33 1 193 268 434 506 1.82e-16 81.3
MsaG046335 MtrunA17_Chr8g0351881 53.623 69 31 1 190 257 315 383 2.07e-16 80.1
MsaG046335 MtrunA17_Chr7g0258181 54.412 68 30 1 191 257 243 310 2.77e-16 79.0
MsaG046335 MtrunA17_Chr2g0329041 57.812 64 26 1 190 252 331 394 3.80e-16 79.7
MsaG046335 MtrunA17_Chr8g0384141 50.667 75 37 0 179 253 102 176 6.54e-16 77.0
MsaG046335 MtrunA17_Chr7g0270871 55.357 56 25 0 202 257 213 268 1.36e-15 77.8
MsaG046335 MtrunA17_Chr3g0087771 54.545 66 29 1 190 254 321 386 2.32e-15 77.0
MsaG046335 MtrunA17_Chr7g0231461 53.030 66 31 0 188 253 294 359 2.59e-15 77.4
MsaG046335 MtrunA17_Chr3g0134001 54.545 66 29 1 198 263 178 242 5.71e-15 74.7
MsaG046335 MtrunA17_Chr1g0213791 53.846 65 29 1 190 253 264 328 9.45e-14 72.0
MsaG046335 MtrunA17_Chr1g0185741 47.222 72 38 0 197 268 190 261 1.19e-13 71.2
MsaG046335 MtrunA17_Chr7g0243041 52.830 53 25 0 198 250 33 85 1.60e-12 65.1
MsaG046335 MtrunA17_Chr4g0054571 44.444 90 48 2 199 286 148 237 3.29e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046335 AT2G24260.1 62.195 246 63 5 137 366 75 306 7.68e-90 275
MsaG046335 AT2G24260.2 72.973 185 41 2 183 366 27 203 1.91e-88 268
MsaG046335 AT4G30980.1 59.302 258 70 8 141 394 83 309 6.75e-83 256
MsaG046335 AT4G30980.2 60.853 258 66 9 141 394 131 357 3.34e-82 256
MsaG046335 AT5G58010.1 56.735 245 80 5 140 370 46 278 2.91e-78 244
MsaG046335 AT4G02590.3 61.828 186 60 4 177 358 66 244 6.72e-57 187
MsaG046335 AT4G02590.2 61.828 186 60 4 177 358 129 307 2.89e-56 187
MsaG046335 AT4G02590.1 61.828 186 60 4 177 358 129 307 2.89e-56 187
MsaG046335 AT1G03040.2 65.806 155 38 3 188 341 136 276 4.46e-51 173
MsaG046335 AT1G03040.1 65.806 155 38 3 188 341 141 281 5.55e-51 173
MsaG046335 AT1G03040.3 59.302 172 52 2 188 341 141 312 4.88e-48 166
MsaG046335 AT5G50915.3 58.824 85 30 2 191 274 136 216 7.90e-24 100
MsaG046335 AT5G50915.4 58.824 85 30 2 191 274 136 216 7.90e-24 100
MsaG046335 AT5G50915.1 58.824 85 30 2 191 274 136 216 9.14e-24 100
MsaG046335 AT5G50915.2 58.824 85 30 2 191 274 136 216 9.14e-24 100
MsaG046335 AT4G34530.1 71.212 66 18 1 191 255 172 237 2.22e-22 97.4
MsaG046335 AT3G07340.2 67.123 73 23 1 184 255 251 323 2.93e-22 98.2
MsaG046335 AT3G07340.1 67.123 73 23 1 184 255 251 323 3.25e-22 98.6
MsaG046335 AT5G48560.2 72.727 66 17 1 191 255 301 366 3.68e-22 98.2
MsaG046335 AT5G48560.1 72.727 66 17 1 191 255 301 366 4.80e-22 98.2
MsaG046335 AT1G10120.2 64.865 74 25 1 183 255 198 271 8.81e-22 96.3
MsaG046335 AT1G10120.1 64.865 74 25 1 183 255 198 271 8.81e-22 96.3
MsaG046335 AT1G68920.3 71.212 66 18 1 191 255 302 367 1.25e-21 97.1
MsaG046335 AT1G68920.1 71.212 66 18 1 191 255 303 368 1.30e-21 96.7
MsaG046335 AT1G68920.4 71.212 66 18 1 191 255 303 368 1.30e-21 96.7
MsaG046335 AT1G68920.2 71.212 66 18 1 191 255 303 368 1.30e-21 96.7
MsaG046335 AT1G59640.1 57.143 84 35 1 173 255 118 201 3.42e-21 92.8
MsaG046335 AT3G23690.1 69.697 66 19 1 191 255 191 256 3.82e-21 94.4
MsaG046335 AT1G59640.2 57.143 84 35 1 173 255 118 201 3.89e-21 94.0
MsaG046335 AT2G18300.1 68.182 66 20 1 191 255 185 250 3.91e-21 94.0
MsaG046335 AT2G18300.2 68.182 66 20 1 191 255 185 250 4.13e-21 94.0
MsaG046335 AT2G18300.3 68.182 66 20 1 191 255 185 250 6.07e-21 93.6
MsaG046335 AT1G26260.3 66.667 66 21 1 191 255 172 237 6.42e-21 93.2
MsaG046335 AT1G26260.5 66.667 66 21 1 191 255 201 266 7.01e-21 93.6
MsaG046335 AT1G26260.4 66.667 66 21 1 191 255 223 288 9.52e-21 93.6
MsaG046335 AT1G26260.1 66.667 66 21 1 191 255 223 288 9.52e-21 93.6
MsaG046335 AT1G26260.6 66.667 66 21 1 191 255 223 288 9.52e-21 93.6
MsaG046335 AT1G26260.2 66.667 66 21 1 191 255 223 288 9.52e-21 93.6
MsaG046335 AT4G36540.1 66.667 66 21 1 191 255 141 206 1.49e-20 91.7
MsaG046335 AT4G36540.2 66.667 66 21 1 191 255 141 206 1.55e-20 91.7
MsaG046335 AT1G73830.2 66.667 66 21 1 191 255 147 212 3.76e-20 89.7
MsaG046335 AT1G73830.1 66.667 66 21 1 191 255 147 212 3.87e-20 89.7
MsaG046335 AT1G25330.1 58.974 78 30 2 191 267 104 180 1.37e-19 87.0
MsaG046335 AT5G62610.2 49.474 95 47 1 162 255 124 218 1.43e-19 88.6
MsaG046335 AT5G62610.1 49.474 95 47 1 162 255 124 218 1.43e-19 88.6
MsaG046335 AT2G14760.1 60.000 65 26 0 190 254 236 300 2.35e-19 88.6
MsaG046335 AT4G33880.1 60.000 65 26 0 190 254 265 329 3.77e-19 88.2
MsaG046335 AT1G18400.2 62.121 66 24 1 191 255 145 210 8.39e-19 85.5
MsaG046335 AT1G27740.1 58.462 65 27 0 190 254 166 230 9.72e-19 85.5
MsaG046335 AT5G43175.1 44.340 106 54 2 164 267 103 205 1.29e-18 84.3
MsaG046335 AT1G09530.3 53.623 69 32 0 188 256 334 402 1.29e-18 88.2
MsaG046335 AT1G09530.2 53.623 69 32 0 188 256 334 402 1.29e-18 88.2
MsaG046335 AT1G09530.5 53.623 69 32 0 188 256 334 402 1.29e-18 88.2
MsaG046335 AT1G09530.1 53.623 69 32 0 188 256 334 402 1.29e-18 88.2
MsaG046335 AT1G09530.4 53.623 69 32 0 188 256 334 402 1.29e-18 88.2
MsaG046335 AT1G09530.6 53.623 69 32 0 188 256 334 402 1.29e-18 88.2
MsaG046335 AT2G42280.1 47.573 103 48 3 157 257 248 346 1.43e-18 86.7
MsaG046335 AT1G18400.1 62.121 66 24 1 191 255 145 210 1.47e-18 85.1
MsaG046335 AT2G20180.7 59.677 62 25 0 194 255 210 271 2.09e-18 86.7
MsaG046335 AT2G20180.1 59.677 62 25 0 194 255 210 271 2.09e-18 86.7
MsaG046335 AT2G20180.5 59.677 62 25 0 194 255 210 271 2.09e-18 86.7
MsaG046335 AT2G42300.4 62.857 70 25 1 187 255 181 250 2.37e-18 84.7
MsaG046335 AT2G20180.4 59.677 62 25 0 194 255 281 342 3.20e-18 86.7
MsaG046335 AT2G20180.6 59.677 62 25 0 194 255 281 342 3.20e-18 86.7
MsaG046335 AT2G20180.2 59.677 62 25 0 194 255 281 342 3.20e-18 86.7
MsaG046335 AT2G20180.3 59.677 62 25 0 194 255 281 342 3.20e-18 86.7
MsaG046335 AT2G42300.1 62.857 70 25 1 187 255 181 250 3.76e-18 85.1
MsaG046335 AT1G66470.1 44.037 109 51 2 173 271 168 276 3.93e-18 84.7
MsaG046335 AT3G57800.2 62.857 70 25 1 187 255 200 269 7.72e-18 84.7
MsaG046335 AT2G42280.3 48.980 98 44 3 157 252 248 341 8.49e-18 84.3
MsaG046335 AT5G37800.1 43.269 104 55 2 169 271 182 282 9.41e-18 83.6
MsaG046335 AT2G20180.8 61.017 59 23 0 194 252 281 339 1.99e-17 83.2
MsaG046335 AT4G09180.1 57.576 66 27 1 190 254 183 248 3.64e-17 81.3
MsaG046335 AT2G14760.3 51.282 78 25 1 190 254 236 313 4.60e-17 82.0
MsaG046335 AT1G35460.1 57.576 66 27 1 190 254 180 245 5.90e-17 80.5
MsaG046335 AT1G51140.1 45.714 105 45 3 154 257 278 371 6.80e-17 82.0
MsaG046335 AT4G36930.1 61.017 59 23 0 197 255 197 255 1.31e-16 81.3
MsaG046335 AT4G00050.2 53.731 67 31 0 188 254 102 168 1.74e-16 79.7
MsaG046335 AT5G67110.1 61.111 54 21 0 202 255 98 151 3.90e-16 77.0
MsaG046335 AT4G00050.1 53.731 67 31 0 188 254 204 270 4.13e-16 79.7
MsaG046335 AT4G00050.3 53.731 67 31 0 188 254 204 270 4.50e-16 79.7
MsaG046335 AT4G28815.2 43.210 81 46 0 170 250 47 127 1.88e-15 75.5
MsaG046335 AT4G28815.1 43.210 81 46 0 170 250 123 203 4.49e-15 75.9
MsaG046335 AT4G28790.1 50.000 68 34 0 188 255 268 335 6.44e-15 76.3
MsaG046335 AT2G46970.2 43.902 82 41 2 185 266 187 263 3.50e-14 73.9
MsaG046335 AT2G46970.1 43.902 82 41 2 185 266 218 294 3.71e-14 73.9
MsaG046335 AT2G46970.3 43.902 82 41 2 185 266 196 272 3.83e-14 73.9
MsaG046335 AT4G28800.1 44.000 75 38 1 194 268 252 322 4.75e-14 73.9
MsaG046335 AT3G21330.1 57.143 56 24 0 198 253 276 331 5.89e-14 73.2
MsaG046335 AT3G59060.3 59.677 62 25 0 194 255 253 314 7.65e-14 73.2
MsaG046335 AT3G59060.2 59.677 62 25 0 194 255 253 314 7.65e-14 73.2
MsaG046335 AT3G59060.4 59.677 62 25 0 194 255 253 314 7.65e-14 73.2
MsaG046335 AT3G59060.1 59.677 62 25 0 194 255 253 314 7.67e-14 73.2
MsaG046335 AT4G28790.2 48.485 66 34 0 186 251 266 331 1.02e-13 72.0
MsaG046335 AT2G43010.2 61.667 60 23 0 194 253 254 313 1.19e-13 72.4
MsaG046335 AT2G43010.1 61.667 60 23 0 194 253 254 313 1.24e-13 72.4
MsaG046335 AT2G43010.3 61.667 60 23 0 194 253 261 320 1.30e-13 72.4
MsaG046335 AT2G43010.4 61.667 60 23 0 194 253 254 313 1.53e-13 72.0
MsaG046335 AT2G43010.5 61.667 60 23 0 194 253 296 355 1.64e-13 72.4
MsaG046335 AT5G01305.1 50.000 62 31 0 189 250 35 96 2.00e-13 68.6
MsaG046335 AT2G43140.1 55.385 65 28 1 190 253 133 197 2.03e-13 69.3
MsaG046335 AT2G43140.2 55.385 65 28 1 190 253 230 294 2.13e-13 70.9
MsaG046335 AT4G28800.2 43.038 79 44 1 194 272 264 341 6.48e-13 70.5
MsaG046335 AT1G05805.1 47.436 78 36 2 177 253 273 346 8.45e-13 69.7
MsaG046335 AT4G00120.1 47.692 65 33 1 200 263 121 185 2.70e-12 65.9
MsaG046335 AT2G42300.2 68.182 44 14 0 187 230 181 224 1.65e-11 64.3
MsaG046335 AT2G42300.5 68.182 44 14 0 187 230 181 224 1.88e-11 63.9
MsaG046335 AT2G42300.3 68.182 44 14 0 187 230 181 224 1.88e-11 63.9
MsaG046335 AT2G46510.1 38.298 94 49 2 168 259 367 453 4.31e-11 65.1

Find 124 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGTTGTTCATCGTAATT+AGG 0.148685 8:+83521766 None:intergenic
ACCATAACAAACCTTTATTA+TGG 0.160798 8:-83521819 MsaT046335.1:CDS
AGGTGTTAACGACGTCGTTC+TGG 0.234818 8:+83521626 None:intergenic
CCACCAATCTTCCTCCTTCC+TGG 0.248518 8:-83521858 MsaT046335.1:CDS
CCCCTCCGTTGCCGTTTGTT+TGG 0.284955 8:+83518024 None:intergenic
TGTCGTTGTTGTTATTGTTT+TGG 0.304419 8:+83521920 None:intergenic
TGAGAGGATGAAAGCTTTGC+AGG 0.309874 8:-83520856 MsaT046335.1:CDS
CCATAACAAACCTTTATTAT+GGG 0.329132 8:-83521818 MsaT046335.1:CDS
ATGCTTCTCACCGCCGATTC+CGG 0.331027 8:-83521685 MsaT046335.1:CDS
CATTGTGAGGCTTCCGTTAG+AGG 0.335163 8:+83517971 None:intergenic
ATAGCAGTAGCAAGAGAAAT+AGG 0.336283 8:+83517864 None:intergenic
GAACGATAGGGGCAGAGTTT+TGG 0.337044 8:-83521319 MsaT046335.1:intron
TCACCGTTAGATGCGGTGAT+AGG 0.343503 8:+83517768 None:intergenic
GTTAGGGTCGAGAGTGTTCC+AGG 0.343687 8:+83521840 None:intergenic
CTCAGCCAAGGCAGAAGGTT+AGG 0.345610 8:-83521246 MsaT046335.1:CDS
AGTGTTACCTTGTTTGCATT+AGG 0.362556 8:+83520825 None:intergenic
AATAAAGGTTTGTTATGGTT+AGG 0.363417 8:+83521823 None:intergenic
GATGCTGTTAACGGACATTC+CGG 0.365589 8:+83517731 None:intergenic
GAATTGGGGTCCCATAATAA+AGG 0.388368 8:+83521808 None:intergenic
CCCATAATAAAGGTTTGTTA+TGG 0.388741 8:+83521818 None:intergenic
GAGCTCCACTCGCCGGAGCT+TGG 0.406538 8:+83521272 None:intergenic
ACCTTCTGCCTTGGCTGAGC+CGG 0.408333 8:+83521250 None:intergenic
CATGTCTTCTTCCATTAGCT+TGG 0.408536 8:+83517932 None:intergenic
ATAAAGGTTTGTTATGGTTA+GGG 0.408820 8:+83521824 None:intergenic
AGCGTTGATCAAAGGGTTCC+TGG 0.409141 8:+83517821 None:intergenic
TGTGTTGTTCATCGTAATTA+GGG 0.410506 8:+83521767 None:intergenic
ATCTTTGACGATGCTGTTAA+CGG 0.422447 8:+83517722 None:intergenic
GCATCAAGTGGCCAAGCTAA+TGG 0.427063 8:-83517943 MsaT046335.1:CDS
GATCTGGTGGTTACGAAACT+TGG 0.434988 8:+83521732 None:intergenic
CCCTTTCGGCGATGCTGTGT+GGG 0.435060 8:+83521200 None:intergenic
GCTCTCTTCAACGGTTTCTC+CGG 0.441559 8:-83521480 MsaT046335.1:CDS
TAGTGAGATACATACCCTTT+CGG 0.447375 8:+83521186 None:intergenic
GTGGAGGAGACCTCCGGAAT+CGG 0.449433 8:+83521672 None:intergenic
CTTGCCCTAACCTTCTGCCT+TGG 0.449584 8:+83521241 None:intergenic
ATGCTGTTAACGGACATTCC+GGG 0.457739 8:+83517732 None:intergenic
GACAGACAAGGCTTCCATGC+TGG 0.473916 8:-83518902 MsaT046335.1:CDS
TCAGCCAAGGCAGAAGGTTA+GGG 0.474534 8:-83521245 MsaT046335.1:CDS
GGTTGAAAATGATGAGGTTG+AGG 0.476792 8:+83521441 None:intergenic
GGTGTTAACGACGTCGTTCT+GGG 0.478074 8:+83521627 None:intergenic
TATTAGCAGCGTTGATCAAA+GGG 0.485300 8:+83517814 None:intergenic
AGGCATGTGGAGGAGACCTC+CGG 0.490529 8:+83521666 None:intergenic
CACCGTTAGATGCGGTGATA+GGG 0.491206 8:+83517769 None:intergenic
AAGGAGGAAGATTGGTGGAG+AGG 0.494703 8:+83521863 None:intergenic
TCCGTTCAAGCTCTCTTCAA+CGG 0.500853 8:-83521489 MsaT046335.1:CDS
CCCACACAGCATCGCCGAAA+GGG 0.501568 8:-83521200 MsaT046335.1:intron
ACCCTTTCGGCGATGCTGTG+TGG 0.505234 8:+83521199 None:intergenic
GTTCCAGGAAGGAGGAAGAT+TGG 0.509194 8:+83521855 None:intergenic
CTACGACTTAGAAGCGGAGT+TGG 0.512114 8:+83517698 None:intergenic
AGCTTGGTTCGTTGCACTCA+CGG 0.516430 8:+83521288 None:intergenic
AGCTCCGGCGAGTGGAGCTC+CGG 0.517913 8:-83521269 MsaT046335.1:CDS
TTATTAGCAGCGTTGATCAA+AGG 0.525147 8:+83517813 None:intergenic
AGTGACTGCGTCCAAACAAA+CGG 0.526034 8:-83518035 MsaT046335.1:CDS
AGGGGAATCTCACTTTGACT+TGG 0.526596 8:+83518845 None:intergenic
CGATTATGTGAAGTTCCTAC+AGG 0.526815 8:-83518869 MsaT046335.1:CDS
AGGGACACCATGGAGAGATC+CGG 0.526947 8:+83521461 None:intergenic
CAAGCTAATGGAAGAAGACA+TGG 0.531731 8:-83517931 MsaT046335.1:CDS
GGTGATGGGCCATCCTCTCC+CGG 0.535124 8:-83517750 MsaT046335.1:CDS
AAAACAGCGTCGTCCTCTAA+CGG 0.537788 8:-83517984 MsaT046335.1:CDS
GAGTGCAACGAACCAAGCTC+CGG 0.538396 8:-83521284 MsaT046335.1:CDS
ACCGTTGAAGAGAGCTTGAA+CGG 0.539080 8:+83521488 None:intergenic
GTGGAGCTCCGGCTCAGCCA+AGG 0.540877 8:-83521258 MsaT046335.1:CDS
TGAGCCGGAGCTCCACTCGC+CGG 0.543105 8:+83521265 None:intergenic
TCCAAACAAACGGCAACGGA+GGG 0.544554 8:-83518025 MsaT046335.1:CDS
GTTAACGGACATTCCGGGAG+AGG 0.549252 8:+83517737 None:intergenic
ACGAACCAAGCTCCGGCGAG+TGG 0.549919 8:-83521277 MsaT046335.1:CDS
TGGGAGGAGTCAAAGTCAGC+AGG 0.551562 8:+83521646 None:intergenic
GTTGAAAATGATGAGGTTGA+GGG 0.552496 8:+83521442 None:intergenic
AGGGGCTGTAGCTCCTCTTG+TGG 0.552998 8:-83518584 MsaT046335.1:CDS
ATGCAATACTTGCAAGGCAA+AGG 0.553212 8:-83517900 MsaT046335.1:CDS
ACGGACATTCCGGGAGAGGA+TGG 0.558510 8:+83517741 None:intergenic
AACCCTATCACCGCATCTAA+CGG 0.559814 8:-83517771 MsaT046335.1:CDS
GGTTTCTCCGGATCTCTCCA+TGG 0.559827 8:-83521468 MsaT046335.1:CDS
ATCACCGCATCTAACGGTGA+TGG 0.562234 8:-83517765 MsaT046335.1:CDS
TCACCGCATCTAACGGTGAT+GGG 0.564709 8:-83517764 MsaT046335.1:CDS
GAGAAGCATAAGAGCGGCGG+CGG 0.565137 8:+83521699 None:intergenic
CTGGTGGTTACGAAACTTGG+AGG 0.565432 8:+83521735 None:intergenic
CTTGATGCTCCGTCATTGTG+AGG 0.566568 8:+83517958 None:intergenic
CAACTGCCACGTGTCACACC+AGG 0.567988 8:-83517839 MsaT046335.1:CDS
ACCCACACAGCATCGCCGAA+AGG 0.569008 8:-83521201 MsaT046335.1:CDS
TATGAATATACTCACGGAGG+AGG 0.570799 8:+83521606 None:intergenic
GGCGATGCTGTGTGGGTCGG+TGG 0.575489 8:+83521207 None:intergenic
CAAGGCAGAAGGTTAGGGCA+AGG 0.577404 8:-83521240 MsaT046335.1:CDS
TCACTTTGACTTGGACCTGT+AGG 0.579189 8:+83518854 None:intergenic
CAAAGTCAGCAGGCATGTGG+AGG 0.582463 8:+83521656 None:intergenic
TCCGGCTCAGCCAAGGCAGA+AGG 0.583403 8:-83521251 MsaT046335.1:CDS
CCAGGAAGGAGGAAGATTGG+TGG 0.584994 8:+83521858 None:intergenic
TCGAGAGTGTTCCAGGAAGG+AGG 0.588906 8:+83521847 None:intergenic
ATCGATGATCTCATCCAGCA+TGG 0.593330 8:+83518888 None:intergenic
AGTCAAAGTCAGCAGGCATG+TGG 0.593631 8:+83521653 None:intergenic
TTTGAGTATGAGCAGATTAG+GGG 0.596866 8:-83518611 MsaT046335.1:CDS
TTCGGCGATGCTGTGTGGGT+CGG 0.599244 8:+83521204 None:intergenic
GTCCAAACAAACGGCAACGG+AGG 0.599582 8:-83518026 MsaT046335.1:CDS
TTTGCCTTGCAAGTATTGCA+TGG 0.601415 8:+83517902 None:intergenic
TTATGTATGGCAGTTACGGA+GGG 0.606270 8:-83520889 MsaT046335.1:intron
TGCGTCCAAACAAACGGCAA+CGG 0.608283 8:-83518029 MsaT046335.1:CDS
TTTATGTATGGCAGTTACGG+AGG 0.609201 8:-83520890 MsaT046335.1:intron
GGGTCGAGAGTGTTCCAGGA+AGG 0.613794 8:+83521844 None:intergenic
GTTTGAGAAAATCGTCGTGA+TGG 0.614530 8:+83521890 None:intergenic
CACAATGACGGAGCATCAAG+TGG 0.616267 8:-83517955 MsaT046335.1:CDS
GATGAGGTTGAGGGACACCA+TGG 0.619769 8:+83521451 None:intergenic
ACTTGTTCCTAATGCAAACA+AGG 0.620351 8:-83520832 MsaT046335.1:intron
GAGGAGACCTCCGGAATCGG+CGG 0.620378 8:+83521675 None:intergenic
TAACGGAAGCCTCACAATGA+CGG 0.625198 8:-83517967 MsaT046335.1:CDS
CTTCTCACCGCCGATTCCGG+AGG 0.626580 8:-83521682 MsaT046335.1:CDS
TTGAGTATGAGCAGATTAGG+GGG 0.628679 8:-83518610 MsaT046335.1:CDS
GCTGCTAATAACAATATCAA+CGG 0.630462 8:-83517801 MsaT046335.1:CDS
TCTGCCATGCAATACTTGCA+AGG 0.632578 8:-83517906 MsaT046335.1:CDS
AGAAAGAATAGAACCTGAGG+AGG 0.639672 8:+83521420 None:intergenic
CCAAACAAACGGCAACGGAG+GGG 0.643439 8:-83518024 MsaT046335.1:CDS
CAGAAGGTTAGGGCAAGGAG+AGG 0.644091 8:-83521235 MsaT046335.1:CDS
AGGGTTCCTGGTGTGACACG+TGG 0.654023 8:+83517833 None:intergenic
TGGCCCATCACCGTTAGATG+CGG 0.659070 8:+83517761 None:intergenic
GAGGACATATCAGCCACAAG+AGG 0.664253 8:+83518571 None:intergenic
TAGCAAGAGAAATAGGCATG+AGG 0.667127 8:+83517871 None:intergenic
GGTGAGAAGCATAAGAGCGG+CGG 0.668806 8:+83521696 None:intergenic
GTTAACGACGTCGTTCTGGG+AGG 0.675970 8:+83521630 None:intergenic
TGTGGATCGACAGACAGACA+AGG 0.682348 8:-83518914 MsaT046335.1:intron
AAGTGGAAAATTTACCTCGG+AGG 0.684381 8:+83518552 None:intergenic
GGCGGTGAGAAGCATAAGAG+CGG 0.689593 8:+83521693 None:intergenic
AAGCTAATGGAAGAAGACAT+GGG 0.700225 8:-83517930 MsaT046335.1:CDS
CGGCGTCTACGACTTAGAAG+CGG 0.702696 8:+83517692 None:intergenic
TGTGGCTGATATGTCCTCCG+AGG 0.708103 8:-83518566 MsaT046335.1:intron
TGAGGAGGTTGAAAATGATG+AGG 0.716913 8:+83521435 None:intergenic
GGAGGGAAAGAATTGCTGAG+AGG 0.735960 8:-83520872 MsaT046335.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 83517698 83522016 83517698 ID=MsaG046335
Chr8 mRNA 83517698 83522016 83517698 ID=MsaT046335.1;Parent=MsaG046335
Chr8 exon 83517698 83518063 83517698 ID=MsaT046335.1.exon7;Parent=MsaT046335.1
Chr8 CDS 83517698 83518063 83517698 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Chr8 exon 83518567 83518635 83518567 ID=MsaT046335.1.exon6;Parent=MsaT046335.1
Chr8 CDS 83518567 83518635 83518567 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Chr8 exon 83518858 83518923 83518858 ID=MsaT046335.1.exon5;Parent=MsaT046335.1
Chr8 CDS 83518858 83518923 83518858 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Chr8 exon 83520833 83520898 83520833 ID=MsaT046335.1.exon4;Parent=MsaT046335.1
Chr8 CDS 83520833 83520898 83520833 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Chr8 exon 83521201 83521332 83521201 ID=MsaT046335.1.exon3;Parent=MsaT046335.1
Chr8 CDS 83521201 83521332 83521201 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Chr8 exon 83521434 83521526 83521434 ID=MsaT046335.1.exon2;Parent=MsaT046335.1
Chr8 CDS 83521434 83521526 83521434 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Chr8 exon 83521621 83522016 83521621 ID=MsaT046335.1.exon1;Parent=MsaT046335.1
Chr8 CDS 83521621 83522016 83521621 ID=cds.MsaT046335.1;Parent=MsaT046335.1
Gene Sequence

>MsaT046335.1

ATGCAATCTCTTAACTCTCTCTTCAACTCTTCTTCTTCTTCTTCTGAAATTCCCATTCCTCTCCAAAATCAGATCCAAAACAATAACAACAACGACACATTCAACCATCACGACGATTTTCTCAAACAAATCCTCTCCACCAATCTTCCTCCTTCCTGGAACACTCTCGACCCTAACCATAACAAACCTTTATTATGGGACCCCAATTCCGATGAAAATGTCACTTTCCCTAATTACGATGAACAACACAACAATCTCGCCTCCAAGTTTCGTAACCACCAGATCACTGACAAAACCGCCGCCGCTCTTATGCTTCTCACCGCCGATTCCGGAGGTCTCCTCCACATGCCTGCTGACTTTGACTCCTCCCAGAACGACGTCGTTAACACCTCCTCCGCTGCTGGTGACGCTTCCGTTCAAGCTCTCTTCAACGGTTTCTCCGGATCTCTCCATGGTGTCCCTCAACCTCATCATTTTCAACCTCCTCAGGGGCAGAGTTTTGGAAGTGGTTCCGTGAGTGCAACGAACCAAGCTCCGGCGAGTGGAGCTCCGGCTCAGCCAAGGCAGAAGGTTAGGGCAAGGAGAGGTCAAGCCACCGACCCACACAGCATCGCCGAAAGGTTACGGAGGGAAAGAATTGCTGAGAGGATGAAAGCTTTGCAGGAACTTGTTCCTAATGCAAACAAGACAGACAAGGCTTCCATGCTGGATGAGATCATCGATTATGTGAAGTTCCTACAGGTCCAAGTCAAAGTTTTGAGTATGAGCAGATTAGGGGGAGCAGGGGCTGTAGCTCCTCTTGTGGCTGATATGTCCTCCGAGGGTGTCAGTGACTGCGTCCAAACAAACGGCAACGGAGGGGTCCACCCACGAAACCCTAAAACAGCGTCGTCCTCTAACGGAAGCCTCACAATGACGGAGCATCAAGTGGCCAAGCTAATGGAAGAAGACATGGGATCTGCCATGCAATACTTGCAAGGCAAAGGTCTTTGCCTCATGCCTATTTCTCTTGCTACTGCTATCTCAACTGCCACGTGTCACACCAGGAACCCTTTGATCAACGCTGCTAATAACAATATCAACGGTAGTTCTAACCCTATCACCGCATCTAACGGTGATGGGCCATCCTCTCCCGGAATGTCCGTTAACAGCATCGTCAAAGATGCCAACTCCGCTTCTAAGTCGTAG
Protein sequence

>MsaT046335.1

MQSLNSLFNSSSSSSEIPIPLQNQIQNNNNNDTFNHHDDFLKQILSTNLPPSWNTLDPNHNKPLLWDPNSDENVTFPNYDEQHNNLASKFRNHQITDKTAAALMLLTADSGGLLHMPADFDSSQNDVVNTSSAAGDASVQALFNGFSGSLHGVPQPHHFQPPQGQSFGSGSVSATNQAPASGAPAQPRQKVRARRGQATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIIDYVKFLQVQVKVLSMSRLGGAGAVAPLVADMSSEGVSDCVQTNGNGGVHPRNPKTASSSNGSLTMTEHQVAKLMEEDMGSAMQYLQGKGLCLMPISLATAISTATCHTRNPLINAANNNINGSSNPITASNGDGPSSPGMSVNSIVKDANSASKS*