AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046353

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046353 MtrunA17_Chr4g0074411 94.362 603 33 1 1 602 1 603 0.0 1127
MsaG046353 MtrunA17_Chr4g0074401 77.066 593 126 5 2 588 1 589 0.0 884
MsaG046353 MtrunA17_Chr2g0304841 42.647 680 297 21 1 602 1 665 4.05e-128 392
MsaG046353 MtrunA17_Chr4g0074421 82.609 184 30 1 67 250 1 182 1.11e-97 296
MsaG046353 MtrunA17_Chr3g0137101 44.118 408 191 8 18 403 8 400 8.03e-93 298
MsaG046353 MtrunA17_Chr1g0149841 47.135 384 176 10 18 383 5 379 6.58e-92 292
MsaG046353 MtrunA17_Chr1g0161431 48.464 293 138 6 18 298 11 302 1.69e-83 273
MsaG046353 MtrunA17_Chr4g0067981 42.559 383 184 7 18 376 31 401 4.38e-82 271
MsaG046353 MtrunA17_Chr2g0294911 46.061 330 163 6 10 327 21 347 1.07e-79 263
MsaG046353 MtrunA17_Chr8g0376881 44.311 334 147 9 16 328 23 338 3.21e-77 254
MsaG046353 MtrunA17_Chr1g0149851 46.483 327 149 5 18 319 5 330 6.53e-77 254
MsaG046353 MtrunA17_Chr7g0275891 47.297 296 136 6 16 293 3 296 1.02e-75 245
MsaG046353 MtrunA17_Chr7g0275401 53.629 248 109 4 18 260 5 251 2.49e-75 242
MsaG046353 MtrunA17_Chr3g0123131 51.908 262 93 4 18 261 13 259 2.93e-75 250
MsaG046353 MtrunA17_Chr3g0134461 46.667 240 78 2 12 250 2 192 9.38e-55 184
MsaG046353 MtrunA17_Chr1g0149831 54.545 187 79 3 77 260 1 184 9.75e-54 182
MsaG046353 MtrunA17_Chr3g0134481 63.710 124 45 0 18 141 8 131 2.91e-47 164
MsaG046353 MtrunA17_Chr3g0134481 59.677 62 24 1 192 252 122 183 8.73e-17 79.0
MsaG046353 MtrunA17_Chr4g0009851 34.274 248 138 3 17 264 10 232 4.46e-44 159
MsaG046353 MtrunA17_Chr4g0009901 36.437 247 132 4 18 264 10 231 1.16e-43 157
MsaG046353 MtrunA17_Chr6g0471041 33.600 250 139 3 16 264 12 235 8.94e-40 150
MsaG046353 MtrunA17_Chr4g0009981 36.634 202 104 4 63 264 2 179 4.44e-34 130
MsaG046353 MtrunA17_Chr8g0391911 32.500 240 149 4 18 250 9 242 6.13e-26 107
MsaG046353 MtrunA17_Chr8g0350761 25.769 260 172 4 17 256 16 274 2.97e-24 106
MsaG046353 MtrunA17_Chr3g0127941 37.903 124 72 2 22 141 56 178 3.02e-23 104
MsaG046353 MtrunA17_Chr6g0473731 34.054 185 90 4 97 280 8 161 1.12e-22 101
MsaG046353 MtrunA17_Chr7g0276361 36.090 133 76 2 19 145 11 140 1.29e-22 102
MsaG046353 MtrunA17_Chr4g0030271 39.231 130 75 2 15 140 81 210 3.32e-22 101
MsaG046353 MtrunA17_Chr4g0011071 33.540 161 83 4 64 224 35 171 4.01e-22 94.0
MsaG046353 MtrunA17_Chr8g0345901 36.000 125 77 1 24 145 38 162 2.59e-21 98.2
MsaG046353 MtrunA17_Chr1g0181811 38.525 122 72 1 22 140 85 206 3.87e-21 98.2
MsaG046353 MtrunA17_Chr4g0031961 61.842 76 26 1 183 258 116 188 8.31e-21 93.6
MsaG046353 MtrunA17_Chr8g0383961 27.347 245 128 5 18 262 14 208 3.97e-20 90.5
MsaG046353 MtrunA17_Chr6g0456631 32.836 134 87 2 18 149 9 141 8.28e-19 84.3
MsaG046353 MtrunA17_Chr7g0255711 53.846 78 36 0 181 258 71 148 8.40e-19 87.4
MsaG046353 MtrunA17_Chr4g0009991 37.324 142 62 3 110 250 1 116 3.53e-18 80.9
MsaG046353 MtrunA17_Chr3g0091641 33.333 123 79 1 22 141 24 146 4.87e-18 87.8
MsaG046353 MtrunA17_Chr4g0061021 33.333 123 79 1 22 141 33 155 5.17e-18 87.8
MsaG046353 MtrunA17_Chr5g0400241 39.516 124 71 2 18 140 14 134 3.05e-16 82.0
MsaG046353 MtrunA17_Chr5g0400241 59.016 61 25 0 194 254 304 364 6.32e-15 77.8
MsaG046353 MtrunA17_Chr4g0054981 62.069 58 22 0 197 254 306 363 4.38e-15 78.6
MsaG046353 MtrunA17_Chr4g0054981 36.290 124 75 2 18 140 14 134 9.06e-15 77.4
MsaG046353 MtrunA17_Chr1g0198601 51.471 68 32 1 194 260 204 271 1.54e-14 75.9
MsaG046353 MtrunA17_Chr4g0062921 46.429 84 41 2 198 280 232 312 3.43e-14 75.1
MsaG046353 MtrunA17_Chr4g0074431 65.957 47 16 0 1 47 3 49 3.41e-13 64.7
MsaG046353 MtrunA17_Chr7g0256551 30.612 147 79 3 24 149 11 155 8.49e-13 68.6
MsaG046353 MtrunA17_Chr4g0040481 40.476 84 48 1 199 282 156 237 3.53e-12 68.6
MsaG046353 MtrunA17_Chr7g0274471 56.667 60 25 1 201 259 218 277 4.07e-12 68.6
MsaG046353 MtrunA17_Chr7g0261911 46.835 79 40 2 197 273 258 336 4.49e-12 68.6
MsaG046353 MtrunA17_Chr2g0324891 41.333 75 43 1 179 253 140 213 5.59e-12 68.2
MsaG046353 MtrunA17_Chr1g0190301 51.562 64 30 1 197 259 171 234 6.74e-12 67.8
MsaG046353 MtrunA17_Chr4g0045361 51.613 62 29 1 198 258 271 332 1.78e-11 66.6
MsaG046353 MtrunA17_Chr5g0414931 26.163 172 107 4 22 173 16 187 1.98e-11 64.3
MsaG046353 MtrunA17_Chr5g0422621 49.180 61 30 1 199 258 196 256 3.18e-11 65.5
MsaG046353 MtrunA17_Chr2g0321461 53.333 60 27 1 197 255 41 100 4.72e-11 65.1
MsaG046353 MtrunA17_Chr5g0417521 52.542 59 27 1 197 254 11 69 5.30e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046353 AT2G25180.1 48.205 390 175 10 2 375 1 379 2.08e-95 304
MsaG046353 AT4G31920.1 51.250 320 132 6 18 326 13 319 1.87e-89 288
MsaG046353 AT5G58080.1 41.099 455 238 10 10 451 6 443 2.41e-82 271
MsaG046353 AT3G16857.1 47.987 298 128 3 16 289 31 325 3.90e-80 266
MsaG046353 AT3G16857.2 47.987 298 128 3 16 289 31 325 1.02e-79 265
MsaG046353 AT1G67710.1 55.253 257 96 5 17 261 5 254 3.51e-77 254
MsaG046353 AT2G01760.1 49.810 263 108 5 18 263 7 262 1.30e-66 222
MsaG046353 AT3G16857.3 47.471 257 108 3 57 289 2 255 7.49e-66 227
MsaG046353 AT2G01760.2 45.017 291 108 6 18 263 7 290 1.26e-60 207
MsaG046353 AT1G67710.2 53.571 196 73 4 77 261 1 189 2.73e-51 183
MsaG046353 AT5G49240.1 40.816 245 135 3 12 250 32 272 1.43e-50 177
MsaG046353 AT2G01760.3 50.000 204 78 5 77 263 1 197 2.08e-45 164
MsaG046353 AT3G62670.1 36.700 297 164 9 12 301 31 310 8.65e-40 151
MsaG046353 AT1G49190.1 29.967 307 168 7 18 301 30 312 1.62e-38 147
MsaG046353 AT1G67710.3 49.143 175 71 4 98 261 13 180 5.06e-37 144
MsaG046353 AT5G07210.2 29.710 276 153 4 22 263 16 284 1.71e-29 124
MsaG046353 AT5G07210.1 28.873 284 153 5 22 263 16 292 5.33e-27 116
MsaG046353 AT2G27070.1 30.182 275 158 6 22 264 16 288 4.38e-25 110
MsaG046353 AT2G46790.1 36.735 147 85 4 1 140 13 158 1.07e-23 105
MsaG046353 AT2G46790.3 36.735 147 85 4 1 140 13 158 1.09e-23 104
MsaG046353 AT5G24470.1 38.095 126 73 2 20 141 48 172 3.07e-23 104
MsaG046353 AT1G49190.2 36.800 125 79 0 18 142 30 154 9.72e-23 103
MsaG046353 AT5G59570.2 61.250 80 30 1 179 258 120 198 1.10e-22 99.4
MsaG046353 AT5G59570.1 61.250 80 30 1 179 258 120 198 1.10e-22 99.4
MsaG046353 AT5G60100.7 38.462 130 76 2 15 140 56 185 4.29e-22 99.4
MsaG046353 AT5G60100.6 38.462 130 76 2 15 140 56 185 4.29e-22 99.4
MsaG046353 AT5G60100.4 38.462 130 76 2 15 140 56 185 4.29e-22 99.4
MsaG046353 AT5G60100.5 38.462 130 76 2 15 140 56 185 9.41e-22 99.4
MsaG046353 AT5G60100.3 38.462 130 76 2 15 140 56 185 9.41e-22 99.4
MsaG046353 AT5G60100.1 38.462 130 76 2 15 140 56 185 9.41e-22 99.4
MsaG046353 AT5G60100.2 39.231 130 75 2 15 140 56 185 1.18e-21 99.4
MsaG046353 AT3G46640.2 62.667 75 27 1 184 258 128 201 1.86e-21 96.3
MsaG046353 AT3G46640.1 62.667 75 27 1 184 258 128 201 1.86e-21 96.3
MsaG046353 AT3G46640.3 62.667 75 27 1 184 258 128 201 2.41e-21 95.9
MsaG046353 AT5G05090.1 59.459 74 30 0 185 258 65 138 2.12e-20 91.7
MsaG046353 AT3G10760.1 63.636 66 24 0 193 258 97 162 1.38e-19 90.9
MsaG046353 AT5G02810.1 36.885 122 74 1 22 140 78 199 1.89e-19 93.2
MsaG046353 AT2G40970.1 63.077 65 24 0 194 258 98 162 5.35e-19 87.4
MsaG046353 AT1G68210.1 28.148 270 162 6 22 260 13 281 1.73e-18 90.1
MsaG046353 AT5G61380.1 34.959 123 77 1 22 141 19 141 1.08e-17 87.4
MsaG046353 AT5G44190.1 58.333 60 25 0 194 253 140 199 2.93e-15 78.6
MsaG046353 AT5G44190.2 58.333 60 25 0 194 253 140 199 3.43e-15 77.8
MsaG046353 AT4G18020.8 37.063 143 81 3 16 151 12 152 3.86e-14 75.5
MsaG046353 AT4G18020.8 57.895 57 22 1 197 253 294 348 4.70e-11 65.9
MsaG046353 AT4G18020.11 37.063 143 81 3 16 151 12 152 3.86e-14 75.5
MsaG046353 AT4G18020.11 57.895 57 22 1 197 253 294 348 4.70e-11 65.9
MsaG046353 AT4G18020.4 37.063 143 81 3 16 151 12 152 3.86e-14 75.5
MsaG046353 AT4G18020.4 57.895 57 22 1 197 253 294 348 4.70e-11 65.9
MsaG046353 AT4G18020.5 37.063 143 81 3 16 151 12 152 3.86e-14 75.5
MsaG046353 AT4G18020.5 57.895 57 22 1 197 253 294 348 4.70e-11 65.9
MsaG046353 AT4G18020.10 37.063 143 81 3 16 151 12 152 3.86e-14 75.5
MsaG046353 AT4G18020.10 57.895 57 22 1 197 253 294 348 4.70e-11 65.9
MsaG046353 AT4G18020.7 37.063 143 81 3 16 151 12 152 3.86e-14 75.5
MsaG046353 AT4G18020.7 57.895 57 22 1 197 253 294 348 4.70e-11 65.9
MsaG046353 AT1G25550.1 44.828 87 46 2 190 275 199 284 4.21e-14 74.3
MsaG046353 AT4G18020.3 37.063 143 81 3 16 151 12 152 5.34e-14 75.5
MsaG046353 AT4G18020.3 57.895 57 22 1 197 253 294 348 4.35e-11 66.2
MsaG046353 AT4G18020.2 37.063 143 81 3 16 151 12 152 5.34e-14 75.5
MsaG046353 AT4G18020.2 57.895 57 22 1 197 253 294 348 4.35e-11 66.2
MsaG046353 AT4G18020.1 37.063 143 81 3 16 151 12 152 5.34e-14 75.5
MsaG046353 AT4G18020.1 57.895 57 22 1 197 253 294 348 4.35e-11 66.2
MsaG046353 AT4G18020.6 37.063 143 81 3 16 151 12 152 5.34e-14 75.5
MsaG046353 AT4G18020.6 57.895 57 22 1 197 253 294 348 4.35e-11 66.2
MsaG046353 AT4G18020.9 37.063 143 81 3 16 151 12 152 5.34e-14 75.5
MsaG046353 AT4G18020.9 57.895 57 22 1 197 253 294 348 4.35e-11 66.2
MsaG046353 AT2G20570.1 48.101 79 38 1 175 253 130 205 2.67e-13 72.8
MsaG046353 AT2G03500.1 45.205 73 39 1 200 271 232 304 2.85e-13 72.8
MsaG046353 AT1G68670.1 44.304 79 42 2 183 260 198 275 3.22e-13 72.0
MsaG046353 AT4G37180.1 43.373 83 46 1 177 258 186 268 1.67e-12 69.7
MsaG046353 AT4G37180.2 43.373 83 46 1 177 258 193 275 1.80e-12 69.7
MsaG046353 AT3G13040.3 51.562 64 30 1 200 262 240 303 9.12e-12 68.2
MsaG046353 AT3G13040.1 51.562 64 30 1 200 262 240 303 9.12e-12 68.2
MsaG046353 AT3G13040.2 51.562 64 30 1 200 262 240 303 9.12e-12 68.2
MsaG046353 AT2G20570.2 62.500 48 18 0 206 253 174 221 1.16e-11 67.8
MsaG046353 AT3G25790.1 51.724 58 27 1 205 261 200 257 1.91e-11 66.6
MsaG046353 AT1G49560.1 45.070 71 38 1 200 269 191 261 3.94e-11 65.1
MsaG046353 AT1G79430.2 39.130 115 49 4 197 309 30 125 3.95e-11 65.5
MsaG046353 AT3G12730.1 45.238 84 43 3 180 262 3 84 4.04e-11 63.9
MsaG046353 AT3G04030.1 53.333 60 27 1 197 255 41 100 4.34e-11 65.5
MsaG046353 AT3G04030.3 53.333 60 27 1 197 255 41 100 4.84e-11 65.5

Find 109 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATGATCAAATTTC+TGG 0.121819 8:-83690806 MsaT046353.1:CDS
AGCTTGAAGCCATCCATTTC+TGG 0.175471 8:+83692462 None:intergenic
GTAGCAGGATTAAAAGATTT+TGG 0.205735 8:+83690878 None:intergenic
CCTCTCCCAGTTCTCACTTT+AGG 0.237398 8:-83690766 MsaT046353.1:CDS
GTAAATCACTCTTCTGTTAG+AGG 0.250033 8:-83690914 MsaT046353.1:CDS
AAACATCGGCTGCTTGAAAC+TGG 0.273444 8:+83691168 None:intergenic
TCCGGTTGGTATGCGTGTTC+TGG 0.308828 8:-83693329 MsaT046353.1:CDS
TGAGGCATCTGAGTCGTTAA+TGG 0.327980 8:+83690530 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.338432 8:-83691352 MsaT046353.1:CDS
TCGTTAATGGACTATTAGTT+TGG 0.342387 8:+83690543 None:intergenic
TTCGACACTTGCTGTTCCTT+CGG 0.359113 8:-83690723 MsaT046353.1:CDS
CGTTAATGGACTATTAGTTT+GGG 0.359733 8:+83690544 None:intergenic
GAGCAACTGCTTGCTCTAGA+TGG 0.366890 8:-83690449 MsaT046353.1:CDS
CTTCTCACATGCAAGTATAA+TGG 0.379037 8:-83693262 MsaT046353.1:intron
CACCTATCAACACATAATTC+AGG 0.390374 8:-83690944 MsaT046353.1:CDS
TGAGCAAGTTGGACTTGAAA+TGG 0.392446 8:-83692436 MsaT046353.1:CDS
GATAAATGTTCCGCTGAAAC+TGG 0.395287 8:+83691089 None:intergenic
AATAAGTGAAGCGGTTAAAC+AGG 0.412727 8:-83690390 MsaT046353.1:intron
AAAGCACTTGTATAATCAAT+TGG 0.414899 8:+83691043 None:intergenic
CTTAAGATTCTTCGAGATCC+AGG 0.425401 8:+83691313 None:intergenic
GTTTCTATAGTTAATCTGCT+TGG 0.431269 8:-83691935 MsaT046353.1:CDS
CTTACTTGACAATGTTGTAA+AGG 0.437919 8:-83690675 MsaT046353.1:CDS
AGTGAAGATGTTACCGAAGA+AGG 0.438022 8:-83693355 MsaT046353.1:CDS
CCACGGATGTTCGAACGCCC+TGG 0.438585 8:+83691119 None:intergenic
ACTGGATCCTCTCCAGCAAA+AGG 0.442603 8:-83691371 MsaT046353.1:CDS
ATCACTCAATGGAGAAGGAT+TGG 0.449366 8:+83690832 None:intergenic
TCTCAAACGACCGTCTAAAC+AGG 0.454349 8:-83691410 MsaT046353.1:CDS
GCCAGAACACGCATACCAAC+CGG 0.455924 8:+83693328 None:intergenic
TAACAGTATCTCTGATGTCT+TGG 0.462540 8:+83691002 None:intergenic
ACCAACCGGAAAACCTTCTT+CGG 0.468377 8:+83693342 None:intergenic
TTCTGTTAGAGGTGCTTATG+TGG 0.470943 8:-83690903 MsaT046353.1:CDS
TGAGAATAGTTGTGTGTCCT+TGG 0.476712 8:-83690420 MsaT046353.1:CDS
TGATGTAAACATGCCAGAAA+TGG 0.478571 8:-83692475 MsaT046353.1:CDS
CTTAAATTCATGTTACTAAG+TGG 0.478822 8:+83691067 None:intergenic
TTCTTCCTTCAACAATACCT+TGG 0.480661 8:-83690618 MsaT046353.1:CDS
GATGGCTGTGGAGGATCGTT+TGG 0.483919 8:-83693380 MsaT046353.1:CDS
TTAGATGAACCATTTGTAGC+AGG 0.485124 8:+83690863 None:intergenic
TTGGTGTGATATAGTGTTGT+CGG 0.488347 8:-83692296 MsaT046353.1:intron
GAGAAAGAAGATTGAAAGCA+AGG 0.491146 8:-83692164 MsaT046353.1:CDS
CACGGATGTTCGAACGCCCT+GGG 0.491277 8:+83691120 None:intergenic
TGGTGTGATATAGTGTTGTC+GGG 0.492589 8:-83692295 MsaT046353.1:intron
TGGAGGAGCTTAAGAACATA+TGG 0.492597 8:-83692198 MsaT046353.1:CDS
GGCTAAAGTAGATGCTGCAC+TGG 0.493832 8:-83691389 MsaT046353.1:CDS
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8:-83691719 MsaT046353.1:CDS
TTATAATTTGTACCTGCAGA+TGG 0.497679 8:+83691682 None:intergenic
ATCAAGGTATCGTTAGTGAT+GGG 0.497943 8:-83692135 MsaT046353.1:CDS
TTAGCTTACTAATGAAAGGC+AGG 0.500886 8:+83692412 None:intergenic
GAATAAAATTTCTCAACTTG+AGG 0.503061 8:+83690512 None:intergenic
TACTACAACTACGAATTCAA+TGG 0.503272 8:-83692550 MsaT046353.1:CDS
AGAATCCAAGGTATTGTTGA+AGG 0.506294 8:+83690613 None:intergenic
CGGGACATGATGATAGAGAA+AGG 0.508606 8:-83692276 MsaT046353.1:CDS
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8:-83692151 MsaT046353.1:CDS
TGAGATTGAAGTGGAGGGAC+TGG 0.515637 8:+83691199 None:intergenic
TCAGACTAGTCTCAAAGTGC+TGG 0.521547 8:-83693293 MsaT046353.1:CDS
TGGTTCCATCACTCTTGCAT+TGG 0.525055 8:+83690490 None:intergenic
CATTGCATCGCTTCCTCCGA+AGG 0.526768 8:+83690707 None:intergenic
TTTGTTAGCTTACTAATGAA+AGG 0.526782 8:+83692408 None:intergenic
AATCAAGGTATCGTTAGTGA+TGG 0.527040 8:-83692136 MsaT046353.1:CDS
TGTTCTTAAGCTCCTCCAAT+CGG 0.529926 8:+83692203 None:intergenic
TTATTCCAATGCAAGAGTGA+TGG 0.531742 8:-83690495 MsaT046353.1:CDS
CGTGACTTGATGAATGTTGA+AGG 0.537018 8:-83691729 MsaT046353.1:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.537224 8:-83691358 MsaT046353.1:CDS
TGGAAGCGCTTGAATTGTTG+AGG 0.538411 8:-83692530 MsaT046353.1:CDS
ATTCTGGTTGATGATGGCTG+TGG 0.540952 8:-83693392 None:intergenic
CTGGATCCTCTCCAGCAAAA+GGG 0.541917 8:-83691370 MsaT046353.1:CDS
CTCCTGAATTATGTGTTGAT+AGG 0.542919 8:+83690942 None:intergenic
TTCAAGCTCCTTGAGCAAGT+TGG 0.547513 8:-83692447 MsaT046353.1:CDS
GGAAGCGCTTGAATTGTTGA+GGG 0.548067 8:-83692529 MsaT046353.1:CDS
GACACTTGCTGTTCCTTCGG+AGG 0.551078 8:-83690720 MsaT046353.1:CDS
AAATGTAGCTAGCCATCTGC+AGG 0.551114 8:-83691694 MsaT046353.1:intron
TTGTTAAAATGATCACTCAA+TGG 0.551340 8:+83690821 None:intergenic
AACATCGGCTGCTTGAAACT+GGG 0.553633 8:+83691169 None:intergenic
ATCGTTAGTGATGGGGTATG+TGG 0.558412 8:-83692127 MsaT046353.1:CDS
CTGGTTGATGATGGCTGTGG+AGG 0.558423 8:-83693389 None:intergenic
TCTAGTCAAACCGATCCGAT+TGG 0.560044 8:-83692218 MsaT046353.1:CDS
TTAAGCTCCTCCAATCGGAT+CGG 0.560387 8:+83692208 None:intergenic
TTTCAAGTCCAACTTGCTCA+AGG 0.564395 8:+83692439 None:intergenic
CTGAAACTGGTTAATTTCCA+CGG 0.564461 8:+83691102 None:intergenic
TCTACTTTAGCCTGTTTAGA+CGG 0.566286 8:+83691400 None:intergenic
TTCACTTATTGTGTCATCCA+AGG 0.566691 8:+83690403 None:intergenic
AAGAAAGGAACAAAGTGAGG+AGG 0.573387 8:-83692050 MsaT046353.1:CDS
ATAAGAAGCAAGAGAATCCA+AGG 0.574630 8:+83690601 None:intergenic
AAATGATCACTCAATGGAGA+AGG 0.574716 8:+83690827 None:intergenic
TAATGAAGACACTAGTTCCA+TGG 0.575646 8:-83690579 MsaT046353.1:CDS
AGTCAAACCGATCCGATTGG+AGG 0.576963 8:-83692215 MsaT046353.1:CDS
CCAGGGCGTTCGAACATCCG+TGG 0.583592 8:-83691119 MsaT046353.1:CDS
GTCATATAAACTCACTGTCA+AGG 0.583894 8:+83691912 None:intergenic
GAAGCGATGCAATGTCAAGA+TGG 0.589336 8:-83690698 MsaT046353.1:CDS
GGGACATGATGATAGAGAAA+GGG 0.593987 8:-83692275 MsaT046353.1:CDS
CTTTCACTTGCAGAAAACAT+CGG 0.600525 8:+83691154 None:intergenic
TTGGGGTACACTTTGTACCA+TGG 0.600741 8:+83690562 None:intergenic
CCTAAAGTGAGAACTGGGAG+AGG 0.607515 8:+83690766 None:intergenic
GCTAACAAGAAGAAAATGCA+TGG 0.607753 8:-83692079 MsaT046353.1:CDS
CTCCAGCAAAAGGGTTCAGT+AGG 0.616386 8:-83691361 MsaT046353.1:CDS
GATTCTTCGAGATCCAGGTA+AGG 0.621330 8:+83691318 None:intergenic
ATGACGAGGAACACTCAACT+CGG 0.622969 8:-83692003 MsaT046353.1:CDS
ATGTCACTAGATGCATATGT+TGG 0.628893 8:+83691280 None:intergenic
GTTAATGGACTATTAGTTTG+GGG 0.635315 8:+83690545 None:intergenic
GATAGAGAAAGGGTGATGAA+AGG 0.639508 8:-83692265 MsaT046353.1:CDS
TCAAGGTATCGTTAGTGATG+GGG 0.646944 8:-83692134 MsaT046353.1:CDS
GTGATGAAAGGTGTGATGAA+AGG 0.648487 8:-83692253 MsaT046353.1:CDS
GTAAACATGCCAGAAATGGA+TGG 0.659081 8:-83692471 MsaT046353.1:CDS
GGATGACACAATAAGTGAAG+CGG 0.659476 8:-83690399 MsaT046353.1:CDS
GTGACTTGATGAATGTTGAA+GGG 0.669266 8:-83691728 MsaT046353.1:CDS
TGGAAATTAACCAGTTTCAG+CGG 0.669997 8:-83691099 MsaT046353.1:CDS
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8:-83691718 MsaT046353.1:CDS
TCATTATCCCAAACAAAACG+CGG 0.696392 8:+83691974 None:intergenic
TACTGATGAAGAGAATGACG+AGG 0.706123 8:-83692017 MsaT046353.1:CDS
GCAAGAGTGATGGAACCACG+TGG 0.841092 8:-83690485 MsaT046353.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 83690303 83693404 83690303 ID=MsaG046353
Chr8 mRNA 83690303 83693404 83690303 ID=MsaT046353.1;Parent=MsaG046353
Chr8 exon 83690303 83690305 83690303 ID=MsaT046353.1.exon6;Parent=MsaT046353.1
Chr8 CDS 83690303 83690305 83690303 ID=cds.MsaT046353.1;Parent=MsaT046353.1
Chr8 exon 83690391 83691446 83690391 ID=MsaT046353.1.exon5;Parent=MsaT046353.1
Chr8 CDS 83690391 83691446 83690391 ID=cds.MsaT046353.1;Parent=MsaT046353.1
Chr8 exon 83691695 83691771 83691695 ID=MsaT046353.1.exon4;Parent=MsaT046353.1
Chr8 CDS 83691695 83691771 83691695 ID=cds.MsaT046353.1;Parent=MsaT046353.1
Chr8 exon 83691927 83692304 83691927 ID=MsaT046353.1.exon3;Parent=MsaT046353.1
Chr8 CDS 83691927 83692304 83691927 ID=cds.MsaT046353.1;Parent=MsaT046353.1
Chr8 exon 83692421 83692573 83692421 ID=MsaT046353.1.exon2;Parent=MsaT046353.1
Chr8 CDS 83692421 83692573 83692421 ID=cds.MsaT046353.1;Parent=MsaT046353.1
Chr8 exon 83693263 83693404 83693263 ID=MsaT046353.1.exon1;Parent=MsaT046353.1
Chr8 CDS 83693263 83693404 83693263 ID=cds.MsaT046353.1;Parent=MsaT046353.1
Gene Sequence

>MsaT046353.1

ATGATGGCTGTGGAGGATCGTTTGGTGAGTGAAGATGTTACCGAAGAAGGTTTTCCGGTTGGTATGCGTGTTCTGGCTGTTGATGACGATCAGACTAGTCTCAAAGTGCTGGAGAAGCATCTTCTCACATGCAAGTATAATGTTACTACAACTACGAATTCAATGGAAGCGCTTGAATTGTTGAGGGAAAAGAGAAGTATGTTTGATCTTATAATTAGTGATGTAAACATGCCAGAAATGGATGGCTTCAAGCTCCTTGAGCAAGTTGGACTTGAAATGGACCTGCCTTTCATTATGTTGTCGGGACATGATGATAGAGAAAGGGTGATGAAAGGTGTGATGAAAGGCGCTTGCGACTATCTAGTCAAACCGATCCGATTGGAGGAGCTTAAGAACATATGGCAACATGTAGTGAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCGTTAGTGATGGGGTATGTGGTCAAGACACCTCATCTGAAAATATTGCTAACAAGAAGAAAATGCATGGACGAAAAAGAAAGGAACAAAGTGAGGAGGAAGAAGCAGATACTGATGAAGAGAATGACGAGGAACACTCAACTCGGAAGAAGCCGCGTTTTGTTTGGGATAATGAGTTGCACAAAAAATTTGTTTCTATAGTTAATCTGCTTGGCCTTGACAAGGCTTATCCTAAAAAAATACGTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTAGCTAGCCATCTGCAGAAATACAGACTTAGTCTCAAACGACCGTCTAAACAGGCTAAAGTAGATGCTGCACTGGATCCTCTCCAGCAAAAGGGTTCAGTAGGTGGATATGGAGATTTTCGCACCTTACCTGGATCTCGAAGAATCTTAAGTAGCACACTGCCAACATATGCATCTAGTGACATGTTTTCTCGGCTGAACGCATCATCTAGTTTGAACATTAGACGAATGAGTTCTTCTGCACCAGTCCCTCCACTTCAATCTCAAAACATTCCCAGTTTCAAGCAGCCGATGTTTTCTGCAAGTGAAAGCTTGAGTTTTTCCCAGGGCGTTCGAACATCCGTGGAAATTAACCAGTTTCAGCGGAACATTTATCCACTTAGTAACATGAATTTAAGTCCAATTGATTATACAAGTGCTTTTACAGTTTCTTCTGGCTTCCAAGACATCAGAGATACTGTTAATGCAAATAGTTCTCTTTCTTGCATCTCAAGCAGTCACCTATCAACACATAATTCAGGAGCATTTGTAAATCACTCTTCTGTTAGAGGTGCTTATGTGGAACCAAAATCTTTTAATCCTGCTACAAATGGTTCATCTAACTTTGCTTCCAATCCTTCTCCATTGAGTGATCATTTTAACAATGATCAAATTTCTGGCAATAGCTTAAATTTTGCCTCTCCCAGTTCTCACTTTAGGAAAATTCCAGTTGATTTTTCTTCGACACTTGCTGTTCCTTCGGAGGAAGCGATGCAATGTCAAGATGGCTTACTTGACAATGTTGTAAAGGCTTCATGTTACACGACGCAGCAGCAAAATGTCAGTTCTTCCTTCAACAATACCTTGGATTCTCTTGCTTCTTATAATGAAGACACTAGTTCCATGGTACAAAGTGTACCCCAAACTAATAGTCCATTAACGACTCAGATGCCTCAAGTTGAGAAATTTTATTCCAATGCAAGAGTGATGGAACCACGTGGTGATTGCTTTTTTGAGCAACTGCTTGCTCTAGATGGATTGATTGAGAATAGTTGTGTGTCCTTGGATGACACAATAAGTGAAGCGGTTAAACAGTAA
Protein sequence

>MsaT046353.1

MMAVEDRLVSEDVTEEGFPVGMRVLAVDDDQTSLKVLEKHLLTCKYNVTTTTNSMEALELLREKRSMFDLIISDVNMPEMDGFKLLEQVGLEMDLPFIMLSGHDDRERVMKGVMKGACDYLVKPIRLEELKNIWQHVVRKKIESKDQNQGIVSDGVCGQDTSSENIANKKKMHGRKRKEQSEEEEADTDEENDEEHSTRKKPRFVWDNELHKKFVSIVNLLGLDKAYPKKIRDLMNVEGLTRENVASHLQKYRLSLKRPSKQAKVDAALDPLQQKGSVGGYGDFRTLPGSRRILSSTLPTYASSDMFSRLNASSSLNIRRMSSSAPVPPLQSQNIPSFKQPMFSASESLSFSQGVRTSVEINQFQRNIYPLSNMNLSPIDYTSAFTVSSGFQDIRDTVNANSSLSCISSSHLSTHNSGAFVNHSSVRGAYVEPKSFNPATNGSSNFASNPSPLSDHFNNDQISGNSLNFASPSSHFRKIPVDFSSTLAVPSEEAMQCQDGLLDNVVKASCYTTQQQNVSSSFNNTLDSLASYNEDTSSMVQSVPQTNSPLTTQMPQVEKFYSNARVMEPRGDCFFEQLLALDGLIENSCVSLDDTISEAVKQ*