AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046354

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046354 MtrunA17_Chr4g0074401 71.157 579 141 4 1 556 21 596 0.0 795
MsaG046354 MtrunA17_Chr4g0074411 70.599 568 148 4 1 549 22 589 0.0 780
MsaG046354 MtrunA17_Chr2g0304841 39.940 671 287 20 1 566 22 681 1.29e-120 372
MsaG046354 MtrunA17_Chr4g0074421 94.505 182 10 0 46 227 1 182 2.29e-113 336
MsaG046354 MtrunA17_Chr3g0137101 52.038 319 137 6 1 303 12 330 2.93e-89 289
MsaG046354 MtrunA17_Chr1g0149841 48.640 331 139 6 1 303 9 336 3.45e-89 284
MsaG046354 MtrunA17_Chr1g0161431 54.472 246 102 5 1 237 15 259 2.02e-80 265
MsaG046354 MtrunA17_Chr4g0067981 44.258 357 166 9 1 330 35 385 2.56e-77 258
MsaG046354 MtrunA17_Chr3g0123131 53.846 247 101 5 1 238 17 259 3.81e-77 254
MsaG046354 MtrunA17_Chr1g0149851 45.783 332 137 7 1 292 9 337 8.15e-75 248
MsaG046354 MtrunA17_Chr8g0376881 48.855 262 118 5 1 257 29 279 3.99e-74 246
MsaG046354 MtrunA17_Chr7g0275401 46.284 296 133 5 1 289 9 285 4.52e-73 235
MsaG046354 MtrunA17_Chr2g0294911 53.361 238 110 1 1 237 33 270 6.29e-73 244
MsaG046354 MtrunA17_Chr7g0275891 44.551 312 153 5 1 292 9 320 1.52e-71 233
MsaG046354 MtrunA17_Chr3g0134461 49.561 228 67 3 1 227 12 192 9.36e-55 184
MsaG046354 MtrunA17_Chr1g0149831 51.546 194 82 4 56 240 1 191 1.36e-52 179
MsaG046354 MtrunA17_Chr4g0009851 37.391 230 121 4 1 230 15 221 9.45e-47 166
MsaG046354 MtrunA17_Chr4g0009901 36.508 252 131 4 1 252 14 236 5.90e-44 157
MsaG046354 MtrunA17_Chr3g0134481 62.500 120 45 0 1 120 12 131 9.95e-44 154
MsaG046354 MtrunA17_Chr3g0134481 60.377 53 21 0 177 229 131 183 3.52e-15 74.3
MsaG046354 MtrunA17_Chr6g0471041 35.341 249 128 6 2 241 19 243 1.86e-40 152
MsaG046354 MtrunA17_Chr4g0009981 37.895 190 94 3 42 230 2 168 6.64e-36 135
MsaG046354 MtrunA17_Chr8g0391911 33.761 234 144 4 1 227 13 242 1.05e-27 112
MsaG046354 MtrunA17_Chr8g0350761 29.921 254 151 7 1 230 21 271 6.44e-27 114
MsaG046354 MtrunA17_Chr3g0127941 32.240 183 112 4 1 174 56 235 1.78e-23 105
MsaG046354 MtrunA17_Chr4g0011071 31.875 160 85 3 43 201 35 171 1.31e-22 95.5
MsaG046354 MtrunA17_Chr6g0473731 38.554 166 84 7 77 242 10 157 2.80e-22 100
MsaG046354 MtrunA17_Chr8g0383961 29.487 234 117 4 1 234 18 203 1.85e-21 94.4
MsaG046354 MtrunA17_Chr4g0009991 39.568 139 61 2 89 227 1 116 2.00e-21 90.1
MsaG046354 MtrunA17_Chr7g0276361 36.154 130 74 2 1 124 14 140 2.88e-21 97.8
MsaG046354 MtrunA17_Chr4g0030271 37.705 122 73 1 1 119 89 210 7.73e-20 94.0
MsaG046354 MtrunA17_Chr6g0456631 32.215 149 98 2 1 147 13 160 1.18e-18 84.0
MsaG046354 MtrunA17_Chr4g0031961 52.381 84 37 2 154 234 104 187 1.19e-18 87.0
MsaG046354 MtrunA17_Chr1g0181811 34.426 122 77 1 1 119 85 206 1.62e-18 89.7
MsaG046354 MtrunA17_Chr8g0345901 33.600 125 80 1 3 124 38 162 9.98e-18 87.0
MsaG046354 MtrunA17_Chr3g0091641 31.707 123 81 1 1 120 24 146 1.86e-17 85.9
MsaG046354 MtrunA17_Chr4g0061021 30.081 123 83 1 1 120 33 155 1.68e-16 82.8
MsaG046354 MtrunA17_Chr7g0255711 58.621 58 24 0 177 234 90 147 1.76e-16 80.5
MsaG046354 MtrunA17_Chr5g0400241 29.665 209 109 5 131 302 264 471 1.24e-15 80.1
MsaG046354 MtrunA17_Chr5g0400241 37.190 121 70 3 1 119 18 134 5.56e-13 71.6
MsaG046354 MtrunA17_Chr4g0054981 48.750 80 31 1 171 240 303 382 1.93e-15 79.3
MsaG046354 MtrunA17_Chr4g0054981 33.333 120 76 2 1 119 18 134 1.02e-11 67.8
MsaG046354 MtrunA17_Chr7g0223971 30.556 180 81 4 88 234 10 178 5.24e-15 77.8
MsaG046354 MtrunA17_Chr7g0274471 62.500 56 20 1 176 230 216 271 1.58e-13 72.8
MsaG046354 MtrunA17_Chr4g0040481 42.857 84 46 1 176 259 156 237 3.05e-13 72.0
MsaG046354 MtrunA17_Chr2g0324891 36.752 117 48 4 114 230 123 213 3.31e-13 72.0
MsaG046354 MtrunA17_Chr1g0190301 60.714 56 21 1 176 230 173 228 4.27e-13 71.2
MsaG046354 MtrunA17_Chr4g0042451 49.153 59 29 1 174 231 13 71 3.53e-12 68.2
MsaG046354 MtrunA17_Chr7g0261911 48.000 75 37 2 178 250 262 336 3.76e-12 68.9
MsaG046354 MtrunA17_Chr2g0291021 55.000 60 25 2 178 236 49 107 4.56e-12 67.8
MsaG046354 MtrunA17_Chr6g0461641 54.237 59 26 1 174 231 38 96 4.99e-12 68.2
MsaG046354 MtrunA17_Chr7g0256551 30.612 147 79 3 3 128 11 155 8.41e-12 65.5
MsaG046354 MtrunA17_Chr5g0417521 50.847 59 28 1 174 231 11 69 1.06e-11 66.6
MsaG046354 MtrunA17_Chr5g0423031 37.662 77 47 1 1 77 14 89 1.50e-11 63.5
MsaG046354 MtrunA17_Chr1g0198601 44.928 69 36 2 167 234 201 268 1.57e-11 66.2
MsaG046354 MtrunA17_Chr4g0074431 100.000 26 0 0 1 26 24 49 1.61e-11 59.7
MsaG046354 MtrunA17_Chr2g0321461 50.847 59 28 1 174 231 41 99 2.59e-11 65.9
MsaG046354 MtrunA17_Chr3g0095611 49.296 71 29 2 167 230 2 72 4.02e-11 62.8
MsaG046354 MtrunA17_Chr4g0062921 31.250 112 70 3 124 234 187 292 4.27e-11 65.5
MsaG046354 MtrunA17_Chr1g0162931 54.545 66 29 1 177 241 17 82 5.02e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046354 AT2G25180.1 46.175 366 169 7 1 341 17 379 2.10e-86 280
MsaG046354 AT1G67710.1 55.242 248 102 4 1 242 11 255 2.07e-78 257
MsaG046354 AT4G31920.1 49.682 314 121 7 1 290 17 317 6.52e-78 256
MsaG046354 AT3G16857.1 47.423 291 128 3 1 267 37 326 3.12e-76 255
MsaG046354 AT5G58080.1 48.553 311 137 8 1 293 18 323 3.17e-76 254
MsaG046354 AT3G16857.2 47.423 291 128 3 1 267 37 326 7.34e-76 254
MsaG046354 AT2G01760.1 49.802 253 111 4 1 239 11 261 1.58e-66 221
MsaG046354 AT3G16857.3 43.706 286 132 4 36 293 2 286 2.85e-63 219
MsaG046354 AT2G01760.2 44.840 281 111 5 1 239 11 289 1.92e-60 206
MsaG046354 AT1G67710.2 52.850 193 82 4 56 242 1 190 2.99e-52 186
MsaG046354 AT5G49240.1 41.277 235 126 5 1 227 42 272 1.58e-48 171
MsaG046354 AT2G01760.3 48.990 198 85 4 56 239 1 196 1.29e-46 166
MsaG046354 AT3G62670.1 39.662 237 125 6 2 230 42 268 1.24e-39 150
MsaG046354 AT1G67710.3 47.701 174 82 4 77 244 13 183 5.97e-38 146
MsaG046354 AT1G49190.1 30.612 294 164 8 3 270 36 315 2.18e-37 143
MsaG046354 AT5G07210.2 29.965 287 155 5 1 246 16 297 9.53e-32 130
MsaG046354 AT5G07210.1 29.153 295 155 6 1 246 16 305 1.97e-29 123
MsaG046354 AT2G27070.1 29.965 287 158 8 1 246 16 300 1.27e-28 120
MsaG046354 AT2G46790.3 38.211 123 71 2 1 119 37 158 1.00e-21 98.6
MsaG046354 AT2G46790.1 38.211 123 71 2 1 119 37 158 1.08e-21 99.0
MsaG046354 AT5G24470.1 36.290 124 74 2 1 120 50 172 1.47e-21 99.0
MsaG046354 AT1G49190.2 36.975 119 75 0 3 121 36 154 3.18e-20 95.1
MsaG046354 AT5G60100.7 38.525 122 72 1 1 119 64 185 1.80e-19 91.7
MsaG046354 AT5G60100.6 38.525 122 72 1 1 119 64 185 1.80e-19 91.7
MsaG046354 AT5G60100.4 38.525 122 72 1 1 119 64 185 1.80e-19 91.7
MsaG046354 AT5G60100.5 38.525 122 72 1 1 119 64 185 3.80e-19 91.3
MsaG046354 AT5G60100.3 38.525 122 72 1 1 119 64 185 3.80e-19 91.3
MsaG046354 AT5G60100.1 38.525 122 72 1 1 119 64 185 3.80e-19 91.3
MsaG046354 AT1G68210.1 24.444 270 170 4 1 237 13 281 5.38e-19 91.7
MsaG046354 AT5G60100.2 38.525 122 72 1 1 119 64 185 5.73e-19 90.9
MsaG046354 AT5G05090.1 50.667 75 37 0 160 234 63 137 1.01e-17 84.0
MsaG046354 AT5G59570.2 40.385 104 60 1 131 234 96 197 1.32e-17 84.3
MsaG046354 AT5G59570.1 40.385 104 60 1 131 234 96 197 1.32e-17 84.3
MsaG046354 AT3G46640.2 58.333 60 25 0 177 236 143 202 2.58e-17 84.0
MsaG046354 AT3G46640.1 58.333 60 25 0 177 236 143 202 2.58e-17 84.0
MsaG046354 AT5G02810.1 34.426 122 77 1 1 119 78 199 3.23e-17 85.9
MsaG046354 AT3G46640.3 60.345 58 23 0 177 234 143 200 3.61e-17 83.2
MsaG046354 AT2G40970.1 58.621 58 24 0 177 234 104 161 4.65e-17 81.6
MsaG046354 AT3G10760.1 54.545 66 30 0 169 234 96 161 6.00e-17 82.8
MsaG046354 AT5G61380.1 31.707 123 81 1 1 120 19 141 2.29e-16 83.2
MsaG046354 AT5G44190.2 61.818 55 21 0 176 230 145 199 1.85e-15 78.6
MsaG046354 AT5G44190.1 61.818 55 21 0 176 230 145 199 1.94e-15 79.0
MsaG046354 AT2G20570.1 56.364 55 24 0 176 230 151 205 9.54e-13 70.9
MsaG046354 AT5G45580.1 50.000 70 32 2 173 241 4 71 9.85e-13 69.3
MsaG046354 AT5G45580.2 50.000 70 32 2 173 241 20 87 1.08e-12 69.3
MsaG046354 AT2G20570.2 62.500 48 18 0 183 230 174 221 3.46e-12 69.3
MsaG046354 AT2G01060.1 55.000 60 25 2 178 236 15 73 5.25e-12 67.4
MsaG046354 AT3G04030.1 52.727 55 25 1 178 231 45 99 2.70e-11 66.2
MsaG046354 AT3G04030.3 52.727 55 25 1 178 231 45 99 2.85e-11 66.2
MsaG046354 AT4G37180.1 51.667 60 28 1 176 234 208 267 3.08e-11 65.9
MsaG046354 AT4G37180.2 51.667 60 28 1 176 234 215 274 3.58e-11 65.5
MsaG046354 AT5G18240.2 54.545 55 24 1 178 231 45 99 4.17e-11 65.5
MsaG046354 AT5G18240.3 54.545 55 24 1 178 231 45 99 4.17e-11 65.5
MsaG046354 AT5G18240.5 54.545 55 24 1 178 231 45 99 4.36e-11 65.5
MsaG046354 AT5G18240.1 54.545 55 24 1 178 231 45 99 4.52e-11 65.5
MsaG046354 AT5G18240.4 54.545 55 24 1 178 231 45 99 4.52e-11 65.5
MsaG046354 AT4G18020.8 36.283 113 69 2 19 130 42 152 6.34e-11 65.5
MsaG046354 AT4G18020.11 36.283 113 69 2 19 130 42 152 6.34e-11 65.5
MsaG046354 AT4G18020.4 36.283 113 69 2 19 130 42 152 6.34e-11 65.5
MsaG046354 AT4G18020.5 36.283 113 69 2 19 130 42 152 6.34e-11 65.5
MsaG046354 AT4G18020.10 36.283 113 69 2 19 130 42 152 6.34e-11 65.5
MsaG046354 AT4G18020.7 36.283 113 69 2 19 130 42 152 6.34e-11 65.5
MsaG046354 AT4G18020.3 36.283 113 69 2 19 130 42 152 7.46e-11 65.5
MsaG046354 AT4G18020.2 36.283 113 69 2 19 130 42 152 7.46e-11 65.5
MsaG046354 AT4G18020.1 36.283 113 69 2 19 130 42 152 7.46e-11 65.5
MsaG046354 AT4G18020.6 36.283 113 69 2 19 130 42 152 7.46e-11 65.5
MsaG046354 AT4G18020.9 36.283 113 69 2 19 130 42 152 7.46e-11 65.5

Find 111 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATTGGTGTCTCGAAAT+TGG 0.196977 8:+83696673 None:intergenic
GATTATCTTGCTTCTTCTAA+TGG 0.232806 8:-83696116 MsaT046354.1:CDS
CCACTTCTGACAGGGTTAAA+AGG 0.242635 8:+83696374 None:intergenic
GGACTTAAATTAATGTTATC+AGG 0.249835 8:+83696569 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.252390 8:-83696824 MsaT046354.1:CDS
AAAAGAAGCCTCGTTTAGTT+TGG 0.279239 8:-83697330 MsaT046354.1:CDS
TAGATGGAGAAATACAATTT+AGG 0.313817 8:-83695770 MsaT046354.1:CDS
CAATGTCAAGATGGCTTACT+TGG 0.323761 8:-83696206 MsaT046354.1:CDS
TTTCCGGGGTATGCGTGTTC+TGG 0.325696 8:-83699638 None:intergenic
TGATTAATGAACTATTAGTT+TGG 0.331944 8:+83696063 None:intergenic
GAGCAATTGCTTAATGTAGA+TGG 0.338130 8:-83695969 MsaT046354.1:CDS
CTCACTCAATGGAACAGAAT+TGG 0.343212 8:+83696334 None:intergenic
ATCATTAGTGATGGGGTATA+TGG 0.343848 8:-83697469 MsaT046354.1:CDS
AAAGAAGCCTCGTTTAGTTT+GGG 0.355798 8:-83697329 MsaT046354.1:CDS
AGACATCGTTGCTGTTCCTT+TGG 0.355803 8:-83696240 MsaT046354.1:CDS
CATTCCTTCCAAGTTCAAAC+TGG 0.363583 8:+83696739 None:intergenic
GATAAATATTCTGCTGAAAC+TGG 0.368030 8:+83696594 None:intergenic
GTTTCTGCAGTTAATCTTGT+CGG 0.368264 8:-83697283 MsaT046354.1:CDS
CACACCATCCAGTTTGAACT+TGG 0.369083 8:-83696747 MsaT046354.1:CDS
CTTCTCACATGCAACTATAA+TGG 0.375217 8:-83699571 MsaT046354.1:intron
TATCAAATGGCTATCTAAAC+AGG 0.381598 8:-83696882 MsaT046354.1:CDS
CATCGTTGCTGTTCCTTTGG+AGG 0.383670 8:-83696237 MsaT046354.1:CDS
AACATTGGTGTCTCGAAATT+GGG 0.390536 8:+83696674 None:intergenic
ATAAATCACTCTCCTGTTGA+AGG 0.396905 8:-83696416 MsaT046354.1:CDS
GATTAATGAACTATTAGTTT+GGG 0.399970 8:+83696064 None:intergenic
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8:-83697755 MsaT046354.1:CDS
TCCAGCACTTTGAGATAAGT+TGG 0.404451 8:+83699601 None:intergenic
AAATGTATCCAGCCATTTGC+AGG 0.408981 8:-83697086 MsaT046354.1:intron
ACTGCATTTGAATCATCAAT+TGG 0.413674 8:+83696548 None:intergenic
TCAAGTTGAGAAGTTCTATT+CGG 0.435476 8:-83696030 MsaT046354.1:CDS
ATATAATTTGTACCTGCAAA+TGG 0.440246 8:+83697074 None:intergenic
TTCACAGCAGAACCTTCAAC+AGG 0.443727 8:+83696404 None:intergenic
TGAATTGTTGAGAGAAAAGA+AGG 0.450895 8:-83697839 MsaT046354.1:CDS
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8:+83697781 None:intergenic
GAGAAGTTCTATTCGGATCA+AGG 0.456955 8:-83696023 MsaT046354.1:CDS
AATGAGACGCAATTGATAGA+TGG 0.460506 8:-83695786 MsaT046354.1:CDS
ACATGGCATTCAAACGTCAA+TGG 0.462461 8:-83696624 MsaT046354.1:CDS
GGAAGTTATTACCCCTGTCT+TGG 0.462540 8:-83695733 MsaT046354.1:CDS
AGAATTGGAAGCAAAGTTAG+AGG 0.465879 8:+83696349 None:intergenic
AGGGTTCAGTAGGTGGATAT+GGG 0.469935 8:-83696823 MsaT046354.1:CDS
CATTGCATCGTTTCCTCCAA+AGG 0.471428 8:+83696224 None:intergenic
TACTACAACTACGAAGTCAT+TGG 0.471545 8:-83697869 MsaT046354.1:CDS
GATACAGAATTGATATCAAA+TGG 0.472061 8:-83696895 MsaT046354.1:CDS
TGACAGTGGCTCTGATGTCT+TGG 0.472341 8:+83696507 None:intergenic
CTTGACTTGATGAATGTTGA+AGG 0.474751 8:-83697121 MsaT046354.1:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8:+83697550 None:intergenic
CAAGGAATCATGGAATCAAA+TGG 0.482889 8:-83696005 MsaT046354.1:CDS
TTCCTCCTTCAACAATACCT+TGG 0.487555 8:-83696138 MsaT046354.1:CDS
AATTTGTACCTGCAAATGGC+TGG 0.488638 8:+83697078 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8:-83697540 MsaT046354.1:CDS
GTTGGATCCCTACCAGCAAA+AGG 0.495630 8:-83696843 MsaT046354.1:CDS
CGATGAAACGACTCCAAGAC+AGG 0.496691 8:+83695720 None:intergenic
CAGCCGACAAAACATGTCAT+TGG 0.496819 8:+83696772 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8:-83697111 MsaT046354.1:CDS
TTGTTAAAATCCTCACTCAA+TGG 0.497939 8:+83696323 None:intergenic
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8:-83697506 MsaT046354.1:CDS
TTAGAGGAACCACTTCTGAC+AGG 0.501081 8:+83696365 None:intergenic
ATAATCCAAGGTATTGTTGA+AGG 0.508220 8:+83696133 None:intergenic
ATCAAGGTATCATTAGTGAT+GGG 0.510346 8:-83697477 MsaT046354.1:CDS
AATCAAGGTATCATTAGTGA+TGG 0.511362 8:-83697478 MsaT046354.1:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8:-83697638 MsaT046354.1:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8:-83697493 MsaT046354.1:CDS
CCATCCAGTTTGAACTTGGA+AGG 0.517311 8:-83696743 MsaT046354.1:CDS
AATGCAGTTACAGATTCCTC+TGG 0.518088 8:-83696533 MsaT046354.1:CDS
CTTTCACTTTCAGAAAACAT+TGG 0.525109 8:+83696659 None:intergenic
TCCAACTTATCTCAAAGTGC+TGG 0.528445 8:-83699602 MsaT046354.1:CDS
TGGCTATCTAAACAGGATAG+AGG 0.531559 8:-83696875 MsaT046354.1:CDS
CAATTCTGTTCCATTGAGTG+AGG 0.541688 8:-83696333 MsaT046354.1:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8:+83697806 None:intergenic
CTATTCGGATCAAGGAATCA+TGG 0.544673 8:-83696015 MsaT046354.1:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.548262 8:-83696830 MsaT046354.1:CDS
CCTTCCAAGTTCAAACTGGA+TGG 0.554980 8:+83696743 None:intergenic
ATGTCATTGGATGCATATGC+TGG 0.565239 8:+83696785 None:intergenic
TAATGGAGACACTAGTTCCA+TGG 0.565315 8:-83696099 MsaT046354.1:CDS
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8:+83697758 None:intergenic
TTGGATCCCTACCAGCAAAA+GGG 0.576563 8:-83696842 MsaT046354.1:CDS
TTGACTTGATGAATGTTGAA+GGG 0.580486 8:-83697120 MsaT046354.1:CDS
GAAACGATGCAATGTCAAGA+TGG 0.582127 8:-83696215 MsaT046354.1:CDS
GGATAGAGGAGATGATGCGT+TGG 0.582696 8:-83696861 MsaT046354.1:CDS
TTGGGCTAAACTTTGTACCA+TGG 0.585108 8:+83696082 None:intergenic
AAGAAAGGTACAAAGTGAGG+AGG 0.585901 8:-83697392 MsaT046354.1:CDS
GAAAGTTCGAGTTCTTTACA+TGG 0.585974 8:-83696641 MsaT046354.1:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8:-83697766 MsaT046354.1:CDS
AACATTTGCATTATTGACAG+TGG 0.591551 8:+83696493 None:intergenic
GGGTTCAGTAGGTGGATATG+GGG 0.592030 8:-83696822 MsaT046354.1:CDS
AGAAGAAGCAAGATAATCCA+AGG 0.597269 8:+83696121 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8:-83697794 MsaT046354.1:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8:-83697560 MsaT046354.1:CDS
GACAGGGGTAATAACTTCCA+CGG 0.600059 8:+83695737 None:intergenic
ATGTTATGAGACTGAAGTGG+AGG 0.606395 8:+83696698 None:intergenic
GAATAGAACTTCTCAACTTG+AGG 0.607973 8:+83696032 None:intergenic
GCTAACAAGAATAAAACGCA+TGG 0.616521 8:-83697421 MsaT046354.1:CDS
AATGTCAAGATGGCTTACTT+GGG 0.618703 8:-83696205 MsaT046354.1:CDS
TACCAGCAAAAGGGTTCAGT+AGG 0.620378 8:-83696833 MsaT046354.1:CDS
TAGAGGAACCACTTCTGACA+GGG 0.620617 8:+83696366 None:intergenic
ATCCAAGGTATTGTTGAAGG+AGG 0.626426 8:+83696136 None:intergenic
GGTAAATGATGACATAGAGA+AGG 0.627198 8:-83697617 MsaT046354.1:CDS
ATTTAGGAGCATCAAAGCCG+TGG 0.634327 8:-83695754 MsaT046354.1:CDS
TCAAGGTATCATTAGTGATG+GGG 0.639460 8:-83697476 MsaT046354.1:CDS
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8:+83697727 None:intergenic
TCTGATGTCTTGGAAGCCAG+AGG 0.643398 8:+83696517 None:intergenic
CTTAAATTAATGTTATCAGG+CGG 0.647690 8:+83696572 None:intergenic
GATGAAACGACTCCAAGACA+GGG 0.649974 8:+83695721 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8:-83697790 MsaT046354.1:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8:+83697731 None:intergenic
TGTTATGAGACTGAAGTGGA+GGG 0.664363 8:+83696699 None:intergenic
GGAATGTTATGAGACTGAAG+TGG 0.672395 8:+83696695 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8:-83697110 MsaT046354.1:CDS
GTGATGAAGAGTGTGATGCA+TGG 0.683135 8:-83697595 MsaT046354.1:CDS
TCATGATCCCAAACTAAACG+AGG 0.706682 8:+83697322 None:intergenic
ATGAAACGACTCCAAGACAG+GGG 0.737951 8:+83695722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 83695710 83699650 83695710 ID=MsaG046354
Chr8 mRNA 83695710 83699650 83695710 ID=MsaT046354.1;Parent=MsaG046354
Chr8 exon 83695710 83695814 83695710 ID=MsaT046354.1.exon6;Parent=MsaT046354.1
Chr8 CDS 83695710 83695814 83695710 ID=cds.MsaT046354.1;Parent=MsaT046354.1
Chr8 exon 83695950 83696918 83695950 ID=MsaT046354.1.exon5;Parent=MsaT046354.1
Chr8 CDS 83695950 83696918 83695950 ID=cds.MsaT046354.1;Parent=MsaT046354.1
Chr8 exon 83697087 83697163 83697087 ID=MsaT046354.1.exon4;Parent=MsaT046354.1
Chr8 CDS 83697087 83697163 83697087 ID=cds.MsaT046354.1;Parent=MsaT046354.1
Chr8 exon 83697275 83697646 83697275 ID=MsaT046354.1.exon3;Parent=MsaT046354.1
Chr8 CDS 83697275 83697646 83697275 ID=cds.MsaT046354.1;Parent=MsaT046354.1
Chr8 exon 83697740 83697892 83697740 ID=MsaT046354.1.exon2;Parent=MsaT046354.1
Chr8 CDS 83697740 83697892 83697740 ID=cds.MsaT046354.1;Parent=MsaT046354.1
Chr8 exon 83699572 83699650 83699572 ID=MsaT046354.1.exon1;Parent=MsaT046354.1
Chr8 CDS 83699572 83699650 83699572 ID=cds.MsaT046354.1;Parent=MsaT046354.1
Gene Sequence

>MsaT046354.1

ATGCGTGTTCTGGCAGTTGATGACGATCCAACTTATCTCAAAGTGCTGGAGAAGCAACTTCTCACATGCAACTATAATGTTACTACAACTACGAAGTCATTGGAAGCGCTTGAATTGTTGAGAGAAAAGAAGGATATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCATGTTGTCGGTAAATGATGACATAGAGAAGGTGATGAAGAGTGTGATGCATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGTCTTGACAAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAGAGATACAGAATTGATATCAAATGGCTATCTAAACAGGATAGAGGAGATGATGCGTTGGATCCCTACCAGCAAAAGGGTTCAGTAGGTGGATATGGGGATTTTTGCACACTGCCAGCATATGCATCCAATGACATGTTTTGTCGGCTGAACACACCATCCAGTTTGAACTTGGAAGGAATGAGTTCTTCTGCACTAGTCCCTCCACTTCAGTCTCATAACATTCCCAATTTCGAGACACCAATGTTTTCTGAAAGTGAAAGTTCGAGTTCTTTACATGGCATTCAAACGTCAATGGAAATTAACCAGTTTCAGCAGAATATTTATCCGCCTGATAACATTAATTTAAGTCCAATTGATGATTCAAATGCAGTTACAGATTCCTCTGGCTTCCAAGACATCAGAGCCACTGTCAATAATGCAAATGTTTCTCTTTCTTGCATCGCAAGCAATCACATACAAACACATAAATCAGAAGCATTTATAAATCACTCTCCTGTTGAAGGTTCTGCTGTGAAAACACAACCTTTTAACCCTGTCAGAAGTGGTTCCTCTAACTTTGCTTCCAATTCTGTTCCATTGAGTGAGGATTTTAACAATGATCAAATGTTGCACAATAACTACAAGTTTACTTCTCCCAGTTCTCATTTTTCTTCGACAGACATCGTTGCTGTTCCTTTGGAGGAAACGATGCAATGTCAAGATGGCTTACTTGGGAATGTTATAAATGCTCCATGTTACTCACAACATCAAAATGCGAGTTCCTCCTTCAACAATACCTTGGATTATCTTGCTTCTTCTAATGGAGACACTAGTTCCATGGTACAAAGTTTAGCCCAAACTAATAGTTCATTAATCACTCAGATGCCTCAAGTTGAGAAGTTCTATTCGGATCAAGGAATCATGGAATCAAATGGTGATTGCTTTATTGAGCAATTGCTTAATGTAGATGGATTCATTCAGAATAGTCGTGCTCAAAATGAGACGCAATTGATAGATGGAGAAATACAATTTAGGAGCATCAAAGCCGTGGAAGTTATTACCCCTGTCTTGGAGTCGTTTCATCGAGTAGTTTAA
Protein sequence

>MsaT046354.1

MRVLAVDDDPTYLKVLEKQLLTCNYNVTTTTKSLEALELLREKKDMFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVMHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQGIISDGVYGQDTSSENIANKNKTHGQKRKVQSEEEEAEQDNDEKRSPQKKPRLVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQRYRIDIKWLSKQDRGDDALDPYQQKGSVGGYGDFCTLPAYASNDMFCRLNTPSSLNLEGMSSSALVPPLQSHNIPNFETPMFSESESSSSLHGIQTSMEINQFQQNIYPPDNINLSPIDDSNAVTDSSGFQDIRATVNNANVSLSCIASNHIQTHKSEAFINHSPVEGSAVKTQPFNPVRSGSSNFASNSVPLSEDFNNDQMLHNNYKFTSPSSHFSSTDIVAVPLEETMQCQDGLLGNVINAPCYSQHQNASSSFNNTLDYLASSNGDTSSMVQSLAQTNSSLITQMPQVEKFYSDQGIMESNGDCFIEQLLNVDGFIQNSRAQNETQLIDGEIQFRSIKAVEVITPVLESFHRVV*