AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046505

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046505 MtrunA17_Chr4g0076321 95.588 136 6 0 1 136 1 136 3.57e-94 267
MsaG046505 MtrunA17_Chr2g0290491 74.615 130 28 2 1 130 1 125 3.37e-64 193
MsaG046505 MtrunA17_Chr5g0446491 74.167 120 27 2 1 116 1 120 5.12e-59 180
MsaG046505 MtrunA17_Chr3g0102351 71.074 121 27 1 1 113 1 121 1.71e-58 179
MsaG046505 MtrunA17_Chr8g0382931 80.412 97 19 0 20 116 29 125 6.36e-54 168
MsaG046505 MtrunA17_Chr8g0384451 78.723 94 20 0 20 113 41 134 1.05e-53 168
MsaG046505 MtrunA17_Chr1g0195851 75.532 94 23 0 20 113 37 130 3.09e-52 163
MsaG046505 MtrunA17_Chr1g0185911 69.492 118 33 2 10 127 22 136 5.34e-52 162
MsaG046505 MtrunA17_Chr7g0263601 64.286 126 42 1 7 129 14 139 1.27e-51 161
MsaG046505 MtrunA17_Chr2g0296321 67.857 112 30 2 6 113 51 160 2.36e-51 163
MsaG046505 MtrunA17_Chr4g0067091 68.224 107 29 1 4 110 12 113 2.13e-50 159
MsaG046505 MtrunA17_Chr4g0076381 65.957 94 32 0 20 113 59 152 5.53e-45 146
MsaG046505 MtrunA17_Chr1g0191981 64.773 88 31 0 21 108 6 93 1.34e-41 134
MsaG046505 MtrunA17_Chr1g0165041 56.436 101 42 1 13 113 2 100 2.48e-39 130
MsaG046505 MtrunA17_Chr5g0446601 45.045 111 56 2 1 108 5 113 3.53e-25 94.7
MsaG046505 MtrunA17_Chr1g0159271 54.667 75 34 0 33 107 1 75 1.16e-23 88.2
MsaG046505 MtrunA17_Chr1g0159291 40.789 76 45 0 24 99 7 82 6.14e-19 75.9
MsaG046505 MtrunA17_Chr1g0158951 50.000 66 33 0 49 114 19 84 1.95e-18 75.1
MsaG046505 MtrunA17_Chr2g0307551 30.952 84 58 0 24 107 16 99 8.21e-14 64.3
MsaG046505 MtrunA17_Chr4g0062271 30.952 84 58 0 24 107 16 99 2.99e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046505 AT4G14540.1 74.576 118 24 2 1 114 1 116 1.45e-59 181
MsaG046505 AT5G47640.1 81.053 95 18 0 20 114 28 122 2.05e-54 169
MsaG046505 AT2G38880.3 64.516 124 40 3 9 130 12 133 2.09e-51 160
MsaG046505 AT2G47810.1 75.532 94 23 0 20 113 52 145 2.21e-51 160
MsaG046505 AT2G38880.2 64.516 124 40 3 9 130 12 133 3.27e-51 159
MsaG046505 AT2G38880.1 64.516 124 40 3 9 130 12 133 3.27e-51 159
MsaG046505 AT2G38880.5 64.516 124 40 3 9 130 12 133 3.27e-51 159
MsaG046505 AT2G13570.1 75.532 94 23 0 20 113 37 130 1.07e-50 160
MsaG046505 AT2G38880.7 64.228 123 40 3 9 130 12 131 9.64e-50 155
MsaG046505 AT2G37060.2 68.807 109 30 2 7 112 16 123 1.59e-49 156
MsaG046505 AT2G37060.3 68.807 109 30 2 7 112 16 123 1.59e-49 156
MsaG046505 AT2G37060.1 68.807 109 30 2 7 112 16 123 1.59e-49 156
MsaG046505 AT2G38880.4 72.549 102 25 2 9 109 12 111 1.63e-49 154
MsaG046505 AT3G53340.5 73.118 93 25 0 20 112 30 122 1.64e-49 156
MsaG046505 AT3G53340.1 73.118 93 25 0 20 112 30 122 1.64e-49 156
MsaG046505 AT2G38880.9 72.549 102 25 2 9 109 12 111 2.69e-49 155
MsaG046505 AT2G38880.10 72.549 102 25 2 9 109 12 111 2.69e-49 155
MsaG046505 AT2G38880.6 72.549 102 25 2 9 109 12 111 2.69e-49 155
MsaG046505 AT2G38880.11 72.549 102 25 2 9 109 12 111 5.65e-49 154
MsaG046505 AT2G38880.8 72.549 102 25 2 9 109 12 111 5.65e-49 154
MsaG046505 AT3G53340.4 73.333 90 24 0 20 109 30 119 1.40e-48 152
MsaG046505 AT3G53340.3 72.527 91 25 0 20 110 30 120 1.35e-47 150
MsaG046505 AT3G53340.2 72.527 91 25 0 20 110 30 120 1.35e-47 150
MsaG046505 AT5G47670.2 62.887 97 36 0 20 116 30 126 2.07e-44 144
MsaG046505 AT5G47670.1 62.887 97 36 0 20 116 59 155 4.14e-44 144
MsaG046505 AT5G47670.3 62.887 97 36 0 20 116 59 155 4.14e-44 144
MsaG046505 AT1G09030.1 56.522 115 47 1 20 131 4 118 8.98e-44 140
MsaG046505 AT1G21970.1 57.143 98 42 0 20 117 60 157 1.17e-40 135
MsaG046505 AT5G08190.2 36.082 97 60 1 24 118 16 112 1.27e-18 77.4
MsaG046505 AT5G08190.1 35.714 98 60 2 24 118 16 113 7.81e-17 72.8
MsaG046505 AT5G23090.4 31.959 97 64 1 24 118 16 112 1.70e-15 69.3
MsaG046505 AT5G23090.5 34.118 85 54 1 24 106 16 100 5.79e-14 65.1
MsaG046505 AT5G23090.2 34.118 85 54 1 24 106 16 100 5.79e-14 65.1
MsaG046505 AT5G23090.1 34.118 85 54 1 24 106 16 100 5.79e-14 65.1
MsaG046505 AT5G23090.3 32.941 85 54 2 25 106 3 87 9.27e-11 56.6

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCAGGTGGTCAAGCTTC+AGG 0.278269 8:+85263640 MsaT046505.1:CDS
GGGTTACCTTCACAAGTATA+GGG 0.382046 8:+85263921 MsaT046505.1:CDS
GATCATGAAGAAAGCTTTAC+CGG 0.392387 8:+85263714 MsaT046505.1:CDS
AGAATATGCAGAGCCTCTTA+AGG 0.393028 8:+85263900 MsaT046505.1:CDS
AGGGTTACCTTCACAAGTAT+AGG 0.429699 8:+85263920 MsaT046505.1:CDS
TTCGATATCTTTGCTCTCGC+CGG 0.458504 8:-85263733 None:intergenic
GAGTTCATAAGCTTCATAAC+AGG 0.460674 8:+85263790 MsaT046505.1:CDS
GATCCTACCAACGTTTGCTA+TGG 0.475579 8:-85263695 None:intergenic
GCAGAATCAGATGATGAATC+AGG 0.475967 8:+85263625 MsaT046505.1:CDS
GAGAAGAGGAAGACAATAAA+CGG 0.477283 8:+85263838 MsaT046505.1:CDS
TTGTGAAGGTAACCCTTAAG+AGG 0.483388 8:-85263913 None:intergenic
GAATATGCAGAGCCTCTTAA+GGG 0.488554 8:+85263901 MsaT046505.1:CDS
AATCAGGTGGTCAAGCTTCA+GGG 0.501937 8:+85263641 MsaT046505.1:CDS
GAATATGATTGGTAGTAGTA+AGG 0.514281 8:+85263972 MsaT046505.1:CDS
GCAGAGAATTACTGCAAGAA+AGG 0.517934 8:+85263665 MsaT046505.1:CDS
CAATTTCCCTATACTTGTGA+AGG 0.523802 8:-85263927 None:intergenic
GTTGCAAATATAATCAACCT+TGG 0.534009 8:-85264023 None:intergenic
ATCCTACCAACGTTTGCTAT+GGG 0.537868 8:-85263694 None:intergenic
AAAAGTGTCAGAAAGAGAAG+AGG 0.551950 8:+85263824 MsaT046505.1:CDS
AGTATTGATCGATTCTTCCA+AGG 0.558864 8:+85264006 MsaT046505.1:CDS
TTCAAAGCCAAGTGTAGTCA+TGG 0.563942 8:-85263878 None:intergenic
GTGTGGGCCATGACTACACT+TGG 0.565546 8:+85263871 MsaT046505.1:CDS
CACAAGTATAGGGAAATTGA+CGG 0.568771 8:+85263931 MsaT046505.1:CDS
TACCAACGTTTGCTATGGGA+AGG 0.599059 8:-85263690 None:intergenic
TTCCCATAGCAAACGTTGGT+AGG 0.621000 8:+85263692 MsaT046505.1:CDS
CTCCTTCCCATAGCAAACGT+TGG 0.626066 8:+85263688 MsaT046505.1:CDS
GGTAGTAGTAAGGAGCAGCA+AGG 0.645799 8:+85263982 MsaT046505.1:CDS
TAAACGGTGATGATCTTGTG+TGG 0.661494 8:+85263854 MsaT046505.1:CDS
AAACGGTGATGATCTTGTGT+GGG 0.691215 8:+85263855 MsaT046505.1:CDS
GAATCAGATGATGAATCAGG+TGG 0.704177 8:+85263628 MsaT046505.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 85263622 85264032 85263622 ID=MsaG046505
Chr8 mRNA 85263622 85264032 85263622 ID=MsaT046505.1;Parent=MsaG046505
Chr8 exon 85263622 85264032 85263622 ID=MsaT046505.1.exon1;Parent=MsaT046505.1
Chr8 CDS 85263622 85264032 85263622 ID=cds.MsaT046505.1;Parent=MsaT046505.1
Gene Sequence

>MsaT046505.1

ATGGCAGAATCAGATGATGAATCAGGTGGTCAAGCTTCAGGGAGCAGAGAATTACTGCAAGAAAGGCTCCTTCCCATAGCAAACGTTGGTAGGATCATGAAGAAAGCTTTACCGGCGAGAGCAAAGATATCGAAAGAAGCTAAAGAAACGATGCAAGAGTGTGTATCAGAGTTCATAAGCTTCATAACAGGTGAAGCATCTGAAAAGTGTCAGAAAGAGAAGAGGAAGACAATAAACGGTGATGATCTTGTGTGGGCCATGACTACACTTGGCTTTGAAGAATATGCAGAGCCTCTTAAGGGTTACCTTCACAAGTATAGGGAAATTGACGGTGACAAGAATTTTTCTATGAATATGATTGGTAGTAGTAAGGAGCAGCAAGGTAGTATTGATCGATTCTTCCAAGGTTGA
Protein sequence

>MsaT046505.1

MAESDDESGGQASGSRELLQERLLPIANVGRIMKKALPARAKISKEAKETMQECVSEFISFITGEASEKCQKEKRKTINGDDLVWAMTTLGFEEYAEPLKGYLHKYREIDGDKNFSMNMIGSSKEQQGSIDRFFQG*