AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046509

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046509 MtrunA17_Chr4g0076381 93.061 245 2 5 1 235 1 240 8.99e-165 454
MsaG046509 MtrunA17_Chr1g0165041 81.188 101 19 0 58 158 4 104 1.19e-58 184
MsaG046509 MtrunA17_Chr2g0290491 61.207 116 41 1 58 173 15 126 4.02e-51 164
MsaG046509 MtrunA17_Chr5g0446491 61.739 115 44 0 41 155 4 118 1.58e-50 162
MsaG046509 MtrunA17_Chr3g0102351 70.103 97 29 0 59 155 26 122 1.49e-49 160
MsaG046509 MtrunA17_Chr7g0263601 68.750 96 30 0 58 153 24 119 2.20e-49 159
MsaG046509 MtrunA17_Chr1g0195851 60.177 113 44 1 42 154 19 130 1.91e-48 157
MsaG046509 MtrunA17_Chr8g0382931 64.948 97 34 0 59 155 27 123 3.30e-46 152
MsaG046509 MtrunA17_Chr4g0067091 48.667 150 75 2 41 189 3 151 6.01e-46 152
MsaG046509 MtrunA17_Chr1g0185911 60.714 112 42 1 40 149 7 118 1.06e-45 150
MsaG046509 MtrunA17_Chr8g0384451 60.606 99 39 0 59 157 39 137 1.35e-45 151
MsaG046509 MtrunA17_Chr4g0076321 65.957 94 32 0 61 154 20 113 4.12e-44 145
MsaG046509 MtrunA17_Chr2g0296321 62.887 97 36 0 58 154 64 160 7.16e-44 148
MsaG046509 MtrunA17_Chr1g0191981 51.087 92 45 0 60 151 4 95 1.95e-33 117
MsaG046509 MtrunA17_Chr5g0446601 45.714 105 57 0 44 148 8 112 9.98e-25 96.7
MsaG046509 MtrunA17_Chr1g0159271 50.000 84 42 0 74 157 1 84 2.76e-24 93.2
MsaG046509 MtrunA17_Chr1g0158951 57.143 63 27 0 90 152 19 81 4.96e-21 84.7
MsaG046509 MtrunA17_Chr1g0159291 40.789 76 45 0 65 140 7 82 7.05e-20 81.3
MsaG046509 MtrunA17_Chr2g0307551 39.744 78 47 0 61 138 12 89 1.83e-17 76.6
MsaG046509 MtrunA17_Chr4g0062271 37.975 79 49 0 60 138 11 89 1.45e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046509 AT5G47670.1 60.849 212 62 6 5 198 4 212 4.84e-74 224
MsaG046509 AT5G47670.3 60.849 212 62 6 5 198 4 212 4.84e-74 224
MsaG046509 AT5G47670.2 65.537 177 41 5 39 198 10 183 8.85e-69 210
MsaG046509 AT1G21970.1 57.923 183 56 4 24 190 12 189 6.48e-64 199
MsaG046509 AT4G14540.1 61.739 115 44 0 42 156 3 117 1.39e-51 165
MsaG046509 AT2G38880.3 60.714 112 44 0 42 153 3 114 5.41e-50 160
MsaG046509 AT2G38880.2 60.714 112 44 0 42 153 3 114 6.57e-50 160
MsaG046509 AT2G38880.1 60.714 112 44 0 42 153 3 114 6.57e-50 160
MsaG046509 AT2G38880.5 60.714 112 44 0 42 153 3 114 6.57e-50 160
MsaG046509 AT5G47640.1 68.687 99 31 0 59 157 26 124 1.01e-49 161
MsaG046509 AT2G37060.2 61.818 110 42 0 44 153 14 123 3.48e-49 159
MsaG046509 AT2G37060.3 61.818 110 42 0 44 153 14 123 3.48e-49 159
MsaG046509 AT2G37060.1 61.818 110 42 0 44 153 14 123 3.48e-49 159
MsaG046509 AT3G53340.5 54.483 145 60 3 58 198 27 169 1.88e-48 157
MsaG046509 AT3G53340.1 54.483 145 60 3 58 198 27 169 1.88e-48 157
MsaG046509 AT2G38880.7 59.259 108 44 0 42 149 3 110 7.78e-47 152
MsaG046509 AT2G38880.4 65.591 93 32 0 58 150 19 111 3.72e-46 149
MsaG046509 AT2G38880.11 58.716 109 45 0 42 150 3 111 5.92e-46 150
MsaG046509 AT2G38880.8 58.716 109 45 0 42 150 3 111 5.92e-46 150
MsaG046509 AT2G38880.9 65.591 93 32 0 58 150 19 111 2.85e-45 149
MsaG046509 AT2G38880.10 65.591 93 32 0 58 150 19 111 2.85e-45 149
MsaG046509 AT2G38880.6 65.591 93 32 0 58 150 19 111 2.85e-45 149
MsaG046509 AT2G13570.1 62.887 97 36 0 59 155 35 131 6.44e-45 150
MsaG046509 AT3G53340.4 66.667 93 31 0 58 150 27 119 9.29e-45 146
MsaG046509 AT2G47810.1 59.615 104 42 0 51 154 42 145 9.47e-45 147
MsaG046509 AT3G53340.3 67.021 94 31 0 58 151 27 120 1.19e-44 147
MsaG046509 AT3G53340.2 67.021 94 31 0 58 151 27 120 1.19e-44 147
MsaG046509 AT1G09030.1 54.167 96 44 0 60 155 3 98 1.15e-37 129
MsaG046509 AT5G23090.5 31.731 104 69 1 60 161 11 114 5.38e-17 75.9
MsaG046509 AT5G23090.2 31.731 104 69 1 60 161 11 114 5.38e-17 75.9
MsaG046509 AT5G23090.1 31.731 104 69 1 60 161 11 114 5.38e-17 75.9
MsaG046509 AT5G23090.4 32.653 98 64 1 60 155 11 108 1.22e-16 75.1
MsaG046509 AT5G08190.2 32.653 98 64 1 60 155 11 108 2.94e-16 73.9
MsaG046509 AT5G08190.1 31.373 102 68 1 60 159 11 112 3.93e-16 73.6
MsaG046509 AT5G23090.3 32.558 86 56 1 78 161 16 101 6.57e-12 62.0

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATGGAATATGGAAATTT+AGG 0.157903 8:+85298691 MsaT046509.1:CDS
CATCCAAATGGAGGGTATTA+TGG 0.196296 8:+85298742 MsaT046509.1:CDS
CCAATACTCATTTATATTGA+TGG 0.259870 8:-85298888 None:intergenic
GGAAAGAGGGGTATGGAATA+TGG 0.311976 8:+85298682 MsaT046509.1:CDS
ATCCTTATCACATTTGCAAT+TGG 0.375002 8:-85298391 None:intergenic
GAGTACATCAGCTTCATAAC+TGG 0.376606 8:+85298487 MsaT046509.1:CDS
CCCACCAACACCAATTCTTC+TGG 0.388520 8:+85296473 MsaT046509.1:CDS
TTATTACGTACCAGAAGAAT+TGG 0.393519 8:-85296483 None:intergenic
TTCCCATAATACCCTCCATT+TGG 0.403389 8:-85298745 None:intergenic
CACCCAGCAGCAATGGAAAC+TGG 0.404381 8:+85296422 None:intergenic
ATCCAAATGGAGGGTATTAT+GGG 0.410012 8:+85298743 MsaT046509.1:CDS
CATCGTTACCGTGAGCTTGA+AGG 0.410024 8:+85298628 MsaT046509.1:CDS
AACTGCAGAAGATGTGCTTT+GGG 0.416101 8:+85298552 MsaT046509.1:CDS
GTATGGAATATGGAAATTTA+GGG 0.421974 8:+85298692 MsaT046509.1:CDS
GAAACTGGAGGAGGGTTTCA+TGG 0.423802 8:+85296437 MsaT046509.1:CDS
TAACTGCAGAAGATGTGCTT+TGG 0.426184 8:+85298551 MsaT046509.1:CDS
TGGTGTTGGTGGGGAGTTTG+CGG 0.433913 8:-85296463 None:intergenic
TTTGTCCCACAGTTTCATAT+TGG 0.443088 8:+85298718 MsaT046509.1:CDS
TTTCATATTGGTCATCCAAA+TGG 0.444564 8:+85298730 MsaT046509.1:CDS
TGGAGGCTCATCTCATTCTC+AGG 0.455169 8:+85298831 MsaT046509.1:CDS
CTTTGGGCTATGAGTAAGCT+TGG 0.475258 8:+85298568 MsaT046509.1:CDS
TGCCAATTGCAAATGTGATA+AGG 0.483489 8:+85298389 MsaT046509.1:CDS
AACAATAGCCACTCATCAGC+TGG 0.494856 8:+85298319 MsaT046509.1:CDS
TGTACTCAGACACACACTCT+TGG 0.503017 8:-85298471 None:intergenic
ACCAGAAGAATTGGTGTTGG+TGG 0.512322 8:-85296474 None:intergenic
CCATCCCTCATCATATAAGT+TGG 0.513039 8:-85298775 None:intergenic
CATTTGCAATTGGCATGAAA+CGG 0.523120 8:-85298381 None:intergenic
CGATGAAGATACATTGTAAG+AGG 0.530199 8:-85298610 None:intergenic
ATGGAATATGGAAATTTAGG+GGG 0.530290 8:+85298694 MsaT046509.1:CDS
AGTTGAACCATTGGGAAAGA+GGG 0.531621 8:+85298669 MsaT046509.1:CDS
CCAGAAGAATTGGTGTTGGT+GGG 0.532173 8:-85296473 None:intergenic
GAGTTGAACCATTGGGAAAG+AGG 0.533551 8:+85298668 MsaT046509.1:CDS
TCCATACCCCTCTTTCCCAA+TGG 0.539795 8:-85298676 None:intergenic
CTCAATGAGAGTTGAACCAT+TGG 0.541003 8:+85298660 MsaT046509.1:CDS
CAATGGTTCAACTCTCATTG+AGG 0.552356 8:-85298659 None:intergenic
CATATTGGTCATCCAAATGG+AGG 0.553538 8:+85298733 MsaT046509.1:CDS
TGGAATATGGAAATTTAGGG+GGG 0.554385 8:+85298695 MsaT046509.1:CDS
ACCATTGGGAAAGAGGGGTA+TGG 0.565142 8:+85298675 MsaT046509.1:CDS
CTGCACCAACTTATATGATG+AGG 0.576417 8:+85298770 MsaT046509.1:CDS
GCAGCAATGGAAACTGGAGG+AGG 0.578206 8:+85296428 None:intergenic
CTTGCAAATGCTGAAGCCAA+TGG 0.581895 8:+85298856 MsaT046509.1:CDS
GTTCTGTTCCAGCTGATGAG+TGG 0.584456 8:-85298327 None:intergenic
ATGAATACGAGGCAGCAAGT+AGG 0.584820 8:+85298286 MsaT046509.1:CDS
TTGATGGTGATGATGACCAT+TGG 0.595421 8:-85298872 None:intergenic
TCAATGAGAGTTGAACCATT+GGG 0.596922 8:+85298661 MsaT046509.1:CDS
TACGATCACCTTCAAGCTCA+CGG 0.607319 8:-85298636 None:intergenic
AACGTTGTCAGAGAGAGCAA+AGG 0.618049 8:+85298521 MsaT046509.1:CDS
ATATTGGTCATCCAAATGGA+GGG 0.621689 8:+85298734 MsaT046509.1:CDS
CGTACCAGAAGAATTGGTGT+TGG 0.622904 8:-85296477 None:intergenic
TATGGAATATGGAAATTTAG+GGG 0.625046 8:+85298693 MsaT046509.1:CDS
AAGACAATGAATGCATTGTG+AGG 0.625324 8:+85298350 MsaT046509.1:CDS
CCAACTTATATGATGAGGGA+TGG 0.628590 8:+85298775 MsaT046509.1:CDS
CAGAAGAATTGGTGTTGGTG+GGG 0.631467 8:-85296472 None:intergenic
AGACAATGAATGCATTGTGA+GGG 0.637888 8:+85298351 MsaT046509.1:CDS
CAGCAATGGAAACTGGAGGA+GGG 0.648465 8:+85296429 None:intergenic
TGCACCAACTTATATGATGA+GGG 0.648613 8:+85298771 MsaT046509.1:CDS
GGATGACCAATATGAAACTG+TGG 0.661508 8:-85298724 None:intergenic
GTTGAACCATTGGGAAAGAG+GGG 0.669657 8:+85298670 MsaT046509.1:CDS
CAGTTTCTGAGATGAATACG+AGG 0.679638 8:+85298275 MsaT046509.1:CDS
CCAGCAGCAATGGAAACTGG+AGG 0.696065 8:+85296425 None:intergenic
GATGACCAATATGAAACTGT+GGG 0.700290 8:-85298723 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 85296434 85298903 85296434 ID=MsaG046509
Chr8 mRNA 85296434 85298903 85296434 ID=MsaT046509.1;Parent=MsaG046509
Chr8 exon 85296434 85296494 85296434 ID=MsaT046509.1.exon1;Parent=MsaT046509.1
Chr8 CDS 85296434 85296494 85296434 ID=cds.MsaT046509.1;Parent=MsaT046509.1
Chr8 exon 85298257 85298903 85298257 ID=MsaT046509.1.exon2;Parent=MsaT046509.1
Chr8 CDS 85298257 85298903 85298257 ID=cds.MsaT046509.1;Parent=MsaT046509.1
Gene Sequence

>MsaT046509.1

ATGGAAACTGGAGGAGGGTTTCATGGCTACCGCAAACTCCCCACCAACACCAATTCTTCTGCAGGGACGTTGAAGCTATCAGTTTCTGAGATGAATACGAGGCAGCAAGTAGGAGAACAGAACAACAATAGCCACTCATCAGCTGGAACAGAACAAGACAATGAATGCATTGTGAGGGAACAAGACCGTTTCATGCCAATTGCAAATGTGATAAGGATCATGCGCAAGATTCTACCACCACATGCAAAAATCTCTGATGATGCAAAAGAAACAATCCAAGAGTGTGTGTCTGAGTACATCAGCTTCATAACTGGAGAAGCTAATGAACGTTGTCAGAGAGAGCAAAGGAAGACAATAACTGCAGAAGATGTGCTTTGGGCTATGAGTAAGCTTGGTTTTGATGATTACATTGAACCTCTTACAATGTATCTTCATCGTTACCGTGAGCTTGAAGGTGATCGTACCTCAATGAGAGTTGAACCATTGGGAAAGAGGGGTATGGAATATGGAAATTTAGGGGGGTTTGTCCCACAGTTTCATATTGGTCATCCAAATGGAGGGTATTATGGGAATGCTGCACCAACTTATATGATGAGGGATGGTAATAATAATGCAACAAATGCTGCTGCTGCTGCTGGAGGCTCATCTCATTCTCAGGCACTTGCAAATGCTGAAGCCAATGGTCATCATCACCATCAATATAAATGA
Protein sequence

>MsaT046509.1

METGGGFHGYRKLPTNTNSSAGTLKLSVSEMNTRQQVGEQNNNSHSSAGTEQDNECIVREQDRFMPIANVIRIMRKILPPHAKISDDAKETIQECVSEYISFITGEANERCQREQRKTITAEDVLWAMSKLGFDDYIEPLTMYLHRYRELEGDRTSMRVEPLGKRGMEYGNLGGFVPQFHIGHPNGGYYGNAAPTYMMRDGNNNATNAAAAAGGSSHSQALANAEANGHHHHQYK*