AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046518

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046518 MtrunA17_Chr4g0075861 91.633 251 21 0 1 251 1 251 1.34e-170 475
MsaG046518 MtrunA17_Chr4g0075971 90.837 251 23 0 1 251 1 251 6.24e-166 472
MsaG046518 MtrunA17_Chr2g0328701 93.578 109 7 0 1 109 18 126 1.30e-71 215
MsaG046518 MtrunA17_Chr2g0328701 39.623 106 54 3 89 192 29 126 5.85e-12 61.2
MsaG046518 MtrunA17_Chr2g0328701 45.588 68 34 1 175 242 32 96 1.49e-11 60.5
MsaG046518 MtrunA17_Chr1g0205821 42.767 159 88 1 89 244 13 171 1.33e-34 122
MsaG046518 MtrunA17_Chr1g0205821 43.275 171 85 4 1 160 1 170 9.12e-32 115
MsaG046518 MtrunA17_Chr3g0077891 43.678 174 89 4 83 247 2 175 3.82e-33 118
MsaG046518 MtrunA17_Chr3g0077891 43.056 144 71 2 25 157 25 168 2.45e-28 106
MsaG046518 MtrunA17_Chr3g0077891 46.377 69 33 1 12 76 99 167 3.20e-11 60.5
MsaG046518 MtrunA17_Chr3g0077861 46.405 153 76 3 12 158 8 160 7.88e-33 117
MsaG046518 MtrunA17_Chr3g0077861 43.373 166 88 3 83 245 2 164 1.99e-29 108
MsaG046518 MtrunA17_Chr3g0077861 51.786 56 27 0 25 80 107 162 5.30e-13 65.1
MsaG046518 MtrunA17_Chr7g0218891 33.913 230 141 5 25 244 31 259 8.38e-33 120
MsaG046518 MtrunA17_Chr7g0218891 34.783 161 98 4 90 244 14 173 1.33e-20 88.2
MsaG046518 MtrunA17_Chr3g0077941 45.614 171 85 4 83 245 2 172 8.48e-33 117
MsaG046518 MtrunA17_Chr3g0077941 41.611 149 77 2 25 163 25 173 4.25e-27 102
MsaG046518 MtrunA17_Chr3g0077911 45.161 155 79 3 10 158 6 160 1.21e-32 117
MsaG046518 MtrunA17_Chr3g0077911 46.497 157 76 5 89 241 8 160 5.61e-30 110
MsaG046518 MtrunA17_Chr1g0206771 41.772 158 78 2 88 240 15 163 1.27e-30 112
MsaG046518 MtrunA17_Chr1g0206771 44.595 148 72 3 13 154 17 160 1.25e-27 104
MsaG046518 MtrunA17_Chr1g0206771 47.222 72 35 1 8 76 91 162 1.39e-13 67.0
MsaG046518 MtrunA17_Chr3g0077881 43.114 167 86 4 83 240 2 168 4.19e-29 108
MsaG046518 MtrunA17_Chr3g0077881 42.282 149 74 4 25 162 25 172 2.72e-28 106
MsaG046518 MtrunA17_Chr1g0205831 40.789 152 78 2 93 244 6 145 3.73e-27 103
MsaG046518 MtrunA17_Chr1g0205831 41.259 143 75 2 25 167 18 151 2.56e-23 93.2
MsaG046518 MtrunA17_Chr1g0209201 40.845 142 79 2 1 140 5 143 2.48e-26 100
MsaG046518 MtrunA17_Chr1g0209201 36.296 135 73 4 89 220 16 140 6.35e-17 75.1
MsaG046518 MtrunA17_Chr1g0209201 47.692 65 33 1 175 239 19 82 1.87e-13 65.9
MsaG046518 MtrunA17_Chr5g0415031 40.000 160 87 2 2 152 33 192 2.06e-25 104
MsaG046518 MtrunA17_Chr5g0415031 35.185 162 100 2 79 235 31 192 1.39e-22 96.7
MsaG046518 MtrunA17_Chr5g0415031 49.398 83 40 1 164 244 33 115 5.63e-17 80.1
MsaG046518 MtrunA17_Chr8g0364231 39.766 171 86 3 11 164 41 211 9.29e-25 102
MsaG046518 MtrunA17_Chr8g0364231 35.256 156 98 2 83 235 36 191 1.35e-20 90.9
MsaG046518 MtrunA17_Chr8g0364231 52.564 78 35 1 167 242 35 112 1.34e-16 79.0
MsaG046518 MtrunA17_Chr6g0452001 39.375 160 87 3 3 152 53 212 1.10e-22 97.1
MsaG046518 MtrunA17_Chr6g0452001 34.177 158 101 1 81 235 55 212 1.55e-19 87.8
MsaG046518 MtrunA17_Chr6g0452001 51.948 77 35 1 167 241 56 132 2.97e-15 75.1
MsaG046518 MtrunA17_Chr6g0452021 38.514 148 84 2 12 152 43 190 2.93e-20 89.4
MsaG046518 MtrunA17_Chr6g0452021 35.484 155 95 3 84 235 38 190 2.86e-18 83.6
MsaG046518 MtrunA17_Chr6g0452021 59.701 67 27 0 175 241 46 112 3.67e-16 77.4
MsaG046518 MtrunA17_Chr1g0206781 53.030 66 31 0 175 240 14 79 3.81e-16 72.4
MsaG046518 MtrunA17_Chr1g0206781 39.130 115 47 2 13 124 12 106 9.72e-15 68.6
MsaG046518 MtrunA17_Chr1g0206781 34.646 127 60 1 81 207 3 106 1.39e-14 68.2
MsaG046518 MtrunA17_Chr1g0205861 49.315 73 37 0 85 157 12 84 3.53e-15 69.7
MsaG046518 MtrunA17_Chr1g0205861 40.244 82 46 1 162 240 3 84 7.96e-13 63.5
MsaG046518 MtrunA17_Chr1g0205811 47.368 76 40 0 175 250 17 92 6.92e-15 69.3
MsaG046518 MtrunA17_Chr1g0205811 40.698 86 51 0 89 174 14 99 1.53e-13 65.5
MsaG046518 MtrunA17_Chr1g0205811 50.000 60 30 0 25 84 30 89 7.71e-12 60.8
MsaG046518 MtrunA17_Chr8g0364021 39.785 93 54 1 83 175 38 128 1.19e-14 71.2
MsaG046518 MtrunA17_Chr8g0364021 40.217 92 48 2 161 250 31 117 6.26e-11 60.8
MsaG046518 MtrunA17_Chr1g0205801 36.364 99 63 0 89 187 12 110 2.71e-13 64.7
MsaG046518 MtrunA17_Chr1g0205801 42.857 70 40 0 175 244 15 84 4.20e-12 61.2
MsaG046518 MtrunA17_Chr7g0218911 44.776 67 37 0 176 242 2 68 2.45e-12 62.4
MsaG046518 MtrunA17_Chr7g0218911 38.961 77 46 1 25 100 14 90 1.73e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046518 AT1G24190.2 39.735 151 82 2 11 152 13 163 6.71e-25 103
MsaG046518 AT1G24190.2 32.919 161 99 2 81 235 6 163 3.34e-19 87.0
MsaG046518 AT1G24190.2 54.286 70 32 0 175 244 17 86 1.88e-14 73.2
MsaG046518 AT1G24190.1 39.735 151 82 2 11 152 13 163 6.71e-25 103
MsaG046518 AT1G24190.1 32.919 161 99 2 81 235 6 163 3.41e-19 87.0
MsaG046518 AT1G24190.1 54.286 70 32 0 175 244 17 86 1.85e-14 73.2
MsaG046518 AT1G24190.3 39.735 151 82 2 11 152 13 163 8.53e-25 103
MsaG046518 AT1G24190.3 32.919 161 99 2 81 235 6 163 3.58e-19 87.0
MsaG046518 AT1G24190.3 54.286 70 32 0 175 244 17 86 2.01e-14 73.2
MsaG046518 AT1G70060.2 38.750 160 88 2 3 152 5 164 2.36e-23 99.4
MsaG046518 AT1G70060.2 33.333 159 102 1 81 235 6 164 4.37e-18 84.0
MsaG046518 AT1G70060.2 55.224 67 30 0 175 241 17 83 8.10e-14 71.2
MsaG046518 AT1G70060.1 38.750 160 88 2 3 152 5 164 2.36e-23 99.4
MsaG046518 AT1G70060.1 33.333 159 102 1 81 235 6 164 4.37e-18 84.0
MsaG046518 AT1G70060.1 55.224 67 30 0 175 241 17 83 8.10e-14 71.2
MsaG046518 AT3G01320.1 40.268 149 81 2 11 151 56 204 5.70e-23 98.2
MsaG046518 AT3G01320.1 33.766 154 100 1 83 234 51 204 3.37e-18 84.3
MsaG046518 AT3G01320.1 54.545 77 33 1 167 241 50 126 2.40e-17 81.6
MsaG046518 AT3G01320.2 40.268 149 81 2 11 151 56 204 6.95e-23 97.8
MsaG046518 AT3G01320.2 33.766 154 100 1 83 234 51 204 4.11e-18 84.0
MsaG046518 AT3G01320.2 54.545 77 33 1 167 241 50 126 2.77e-17 81.6
MsaG046518 AT5G15020.2 38.926 149 84 2 11 152 51 199 3.37e-22 95.9
MsaG046518 AT5G15020.2 34.416 154 100 1 83 235 46 199 7.88e-20 89.0
MsaG046518 AT5G15020.2 51.316 76 35 1 168 241 46 121 5.57e-15 74.7
MsaG046518 AT5G15020.1 38.926 149 84 2 11 152 51 199 3.64e-22 95.9
MsaG046518 AT5G15020.1 34.416 154 100 1 83 235 46 199 8.43e-20 89.0
MsaG046518 AT5G15020.1 51.316 76 35 1 168 241 46 121 6.30e-15 74.7
MsaG046518 AT1G59890.1 39.752 161 87 3 12 162 45 205 4.32e-22 95.5
MsaG046518 AT1G59890.1 37.162 148 92 1 89 235 45 192 7.66e-19 86.3
MsaG046518 AT1G59890.1 50.746 67 33 0 175 241 48 114 9.52e-13 68.2
MsaG046518 AT1G59890.2 39.752 161 87 3 12 162 45 205 4.41e-22 95.5
MsaG046518 AT1G59890.2 37.162 148 92 1 89 235 45 192 7.60e-19 86.3
MsaG046518 AT1G59890.2 50.746 67 33 0 175 241 48 114 9.62e-13 68.2
MsaG046518 AT1G59890.4 39.752 161 87 3 12 162 45 205 4.53e-22 95.5
MsaG046518 AT1G59890.4 37.162 148 92 1 89 235 45 192 8.57e-19 85.9
MsaG046518 AT1G59890.4 50.746 67 33 0 175 241 48 114 1.06e-12 67.8
MsaG046518 AT1G59890.3 39.752 161 87 3 12 162 45 205 4.53e-22 95.5
MsaG046518 AT1G59890.3 37.162 148 92 1 89 235 45 192 7.73e-19 85.9
MsaG046518 AT1G59890.3 50.746 67 33 0 175 241 48 114 9.97e-13 68.2
MsaG046518 AT1G24230.1 33.758 157 91 3 89 244 14 158 2.35e-20 87.4
MsaG046518 AT1G24230.1 27.111 225 149 4 11 230 13 227 2.87e-19 84.3
MsaG046518 AT1G10450.3 38.776 147 83 2 12 151 84 230 2.54e-20 90.5
MsaG046518 AT1G10450.3 37.838 148 89 2 89 234 84 230 1.79e-19 87.8
MsaG046518 AT1G10450.3 49.254 67 34 0 175 241 87 153 1.01e-12 68.2
MsaG046518 AT1G10450.2 38.776 147 83 2 12 151 33 179 3.35e-20 90.1
MsaG046518 AT1G10450.2 37.838 148 89 2 89 234 33 179 1.68e-19 88.2
MsaG046518 AT1G10450.2 49.254 67 34 0 175 241 36 102 1.02e-12 68.2
MsaG046518 AT1G10450.1 38.776 147 83 2 12 151 33 179 3.35e-20 90.1
MsaG046518 AT1G10450.1 37.838 148 89 2 89 234 33 179 1.68e-19 88.2
MsaG046518 AT1G10450.1 49.254 67 34 0 175 241 36 102 1.02e-12 68.2
MsaG046518 AT1G27260.1 34.615 156 91 3 90 236 4 157 3.33e-18 80.9
MsaG046518 AT1G27260.1 31.210 157 95 2 13 156 4 160 9.44e-15 71.6
MsaG046518 AT1G24250.1 30.270 185 100 4 2 157 4 188 5.76e-17 78.2
MsaG046518 AT1G24250.1 28.235 170 97 4 88 233 13 181 2.84e-12 65.1
MsaG046518 AT1G70030.1 34.416 154 87 5 12 155 11 160 1.09e-16 75.5
MsaG046518 AT1G70030.1 33.333 159 97 4 81 234 3 157 2.80e-15 71.6
MsaG046518 AT1G23810.1 30.508 177 105 3 2 160 4 180 1.44e-16 77.0
MsaG046518 AT1G23810.1 29.518 166 103 4 88 240 13 177 1.57e-13 68.6
MsaG046518 AT1G24200.1 35.570 149 90 2 89 234 14 159 1.48e-16 75.9
MsaG046518 AT1G24200.1 34.694 147 90 2 11 151 13 159 6.47e-16 74.3
MsaG046518 AT1G24200.1 46.053 76 39 1 167 240 7 82 8.46e-13 65.9
MsaG046518 AT1G27280.1 34.014 147 92 2 100 241 4 150 2.64e-15 73.2
MsaG046518 AT4G12020.11 51.515 66 31 1 176 241 312 376 8.70e-13 68.2
MsaG046518 AT4G12020.11 35.345 116 56 3 12 121 308 410 4.97e-11 63.2
MsaG046518 AT4G12020.6 51.515 66 31 1 176 241 312 376 9.08e-13 68.2
MsaG046518 AT4G12020.6 35.345 116 56 3 12 121 308 410 5.18e-11 63.2
MsaG046518 AT4G12020.8 51.515 66 31 1 176 241 312 376 9.08e-13 68.2
MsaG046518 AT4G12020.8 35.345 116 56 3 12 121 308 410 5.18e-11 63.2
MsaG046518 AT4G12020.7 51.515 66 31 1 176 241 312 376 9.09e-13 68.2
MsaG046518 AT4G12020.7 35.345 116 56 3 12 121 308 410 5.19e-11 63.2
MsaG046518 AT4G12020.1 51.515 66 31 1 176 241 312 376 9.09e-13 68.2
MsaG046518 AT4G12020.1 35.345 116 56 3 12 121 308 410 5.19e-11 63.2
MsaG046518 AT4G12020.9 51.515 66 31 1 176 241 312 376 9.09e-13 68.2
MsaG046518 AT4G12020.9 35.345 116 56 3 12 121 308 410 5.19e-11 63.2
MsaG046518 AT4G12020.5 51.515 66 31 1 176 241 245 309 9.10e-13 68.2
MsaG046518 AT4G12020.5 35.345 116 56 3 12 121 241 343 5.20e-11 63.2
MsaG046518 AT4G12020.10 51.515 66 31 1 176 241 312 376 9.13e-13 68.2
MsaG046518 AT4G12020.10 35.345 116 56 3 12 121 308 410 5.21e-11 63.2
MsaG046518 AT4G12020.3 51.515 66 31 1 176 241 312 376 9.13e-13 68.2
MsaG046518 AT4G12020.3 35.345 116 56 3 12 121 308 410 5.21e-11 63.2
MsaG046518 AT4G12020.4 51.515 66 31 1 176 241 312 376 9.14e-13 68.2
MsaG046518 AT4G12020.4 35.345 116 56 3 12 121 308 410 5.21e-11 63.2
MsaG046518 AT4G12020.2 51.515 66 31 1 176 241 312 376 9.14e-13 68.2
MsaG046518 AT4G12020.2 35.345 116 56 3 12 121 308 410 5.22e-11 63.2
MsaG046518 AT5G15025.1 50.746 67 33 0 175 241 50 116 1.19e-12 63.5
MsaG046518 AT5G15025.1 45.349 86 43 2 2 83 36 121 5.90e-12 61.6
MsaG046518 AT1G70030.2 33.333 147 66 6 12 155 22 139 5.55e-12 62.0
MsaG046518 AT5G35610.1 40.244 82 42 1 176 250 14 95 2.38e-11 60.8
MsaG046518 AT5G15040.1 44.776 67 37 0 175 241 6 72 2.86e-11 58.9
MsaG046518 AT5G15040.3 44.776 67 37 0 175 241 6 72 2.86e-11 58.9
MsaG046518 AT5G15040.2 44.776 67 37 0 175 241 6 72 2.86e-11 58.9
MsaG046518 AT1G27250.1 46.154 65 35 0 175 239 24 88 4.14e-11 59.7
MsaG046518 AT1G27270.1 32.164 171 93 4 10 157 12 182 5.12e-11 61.6
MsaG046518 AT1G24220.2 25.506 247 136 8 3 224 5 228 5.85e-11 62.8
MsaG046518 AT1G24220.1 25.506 247 136 8 3 224 5 228 5.85e-11 62.8

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCAAAATTAAATGTTTA+TGG 0.087759 8:-85419894 None:intergenic
AGACCATACAAATTTAATTT+TGG 0.091325 8:+85420380 MsaT046518.1:CDS
AGGCCATAAACATTTAATTT+TGG 0.093733 8:+85419891 MsaT046518.1:CDS
AATGTGAATCCGTATCTTTC+TGG 0.260796 8:-85420171 None:intergenic
GGGCATGTCGATTTACTTTC+GGG 0.267983 8:+85420132 MsaT046518.1:CDS
ATTGCGATGAAGCTATTTAA+AGG 0.296597 8:+85420111 MsaT046518.1:CDS
AGTATGATTTCATAATTCTT+TGG 0.310501 8:-85419931 None:intergenic
AGAGTGCATGAGTTGTTTAA+AGG 0.320962 8:+85419871 MsaT046518.1:CDS
AGGGCATGTCGATTTACTTT+CGG 0.325603 8:+85420131 MsaT046518.1:CDS
CAGAAGTCATGAGTGATTAC+AGG 0.353321 8:+85419815 MsaT046518.1:CDS
ATCCCAAAATTAAATTTGTA+TGG 0.371343 8:-85420383 None:intergenic
TTGCGATGAAGCTATTTAAA+GGG 0.398600 8:+85420112 MsaT046518.1:CDS
ACTCTTGCTTTGACACCTCT+TGG 0.426483 8:-85419853 None:intergenic
TCATGGCAATGAAGCTCAAA+TGG 0.432218 8:-85420444 None:intergenic
AGTTGTGTTTCAAGATAAGA+TGG 0.435039 8:+85420014 MsaT046518.1:CDS
GAGCTTCATTGCCATGAGAC+TGG 0.471478 8:+85420450 MsaT046518.1:CDS
AGCCGTGTTTCATGATAAGT+TGG 0.480965 8:+85420263 MsaT046518.1:CDS
AGTGTGATTTGATGTTCCTC+TGG 0.511878 8:-85420420 None:intergenic
TACTTCTTGCCAGAAAGATA+CGG 0.529766 8:+85420162 MsaT046518.1:CDS
CAAGGCTCAAGGAATTGATA+CGG 0.538557 8:+85420323 MsaT046518.1:CDS
TGTTTATGATCTTGCAACAG+TGG 0.557427 8:-85420195 None:intergenic
GATGCTTTGTTTGATGATGC+AGG 0.558023 8:+85419739 MsaT046518.1:CDS
TGAAATCATACTTCCTCCAT+CGG 0.558415 8:+85419942 MsaT046518.1:CDS
AATTGATACGGGAGGTGTTG+CGG 0.560033 8:+85420335 MsaT046518.1:CDS
GTCATGAGTGATTACAGGGA+TGG 0.580278 8:+85419820 MsaT046518.1:CDS
ATCAAACAAAGCATCGCTTG+TGG 0.584167 8:-85419731 None:intergenic
ATCACAAATCACAAGGCTCA+AGG 0.601072 8:+85420312 MsaT046518.1:CDS
AAGGCTCAAGGAATTGATAC+GGG 0.604072 8:+85420324 MsaT046518.1:CDS
TTCCAACTTATCATGAAACA+CGG 0.609205 8:-85420265 None:intergenic
TTGATGTTCCTCTGGCAGCA+AGG 0.614555 8:-85420412 None:intergenic
TAAACTACTAACCAGTCTCA+TGG 0.620534 8:-85420461 None:intergenic
AGAAGTCATGAGTGATTACA+GGG 0.629088 8:+85419816 MsaT046518.1:CDS
ATTCAATGCCTTGCTGCCAG+AGG 0.639665 8:+85420404 MsaT046518.1:CDS
ACAAGTTATCACAAATCACA+AGG 0.640420 8:+85420305 MsaT046518.1:CDS
CTGTTGCAAGATCATAAACA+AGG 0.655865 8:+85420198 MsaT046518.1:CDS
GCTCAAGGAATTGATACGGG+AGG 0.704641 8:+85420327 MsaT046518.1:CDS
GATGGACGAATTGATCCAAG+AGG 0.740587 8:+85419838 MsaT046518.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 85419721 85420476 85419721 ID=MsaG046518
Chr8 mRNA 85419721 85420476 85419721 ID=MsaT046518.1;Parent=MsaG046518
Chr8 exon 85419721 85420476 85419721 ID=MsaT046518.1.exon1;Parent=MsaT046518.1
Chr8 CDS 85419721 85420476 85419721 ID=cds.MsaT046518.1;Parent=MsaT046518.1
Gene Sequence

>MsaT046518.1

ATGAATAGCTCCACAAGCGATGCTTTGTTTGATGATGCAGGTGCGTATGACCATGCAGTAAAATCTGCATGTCAAGAAAAGTATTCTGAATTTGCAGAAGTCATGAGTGATTACAGGGATGGACGAATTGATCCAAGAGGTGTCAAAGCAAGAGTGCATGAGTTGTTTAAAGGCCATAAACATTTAATTTTGGGAATCAACAACATCATGCCAAAGAATTATGAAATCATACTTCCTCCATCGGATGAAAAACTGAATCGACAAGATGCAACAACTTTTCTCAAACAAGTAAAAGTTGTGTTTCAAGATAAGATGGAAAAATATTATGAGTTTCTACAAGTCATACAAGACTACATGAATCTCACAATTGATATATTAGATGTCATCAAAATTGCGATGAAGCTATTTAAAGGGCATGTCGATTTACTTTCGGGATTCAACTACTTCTTGCCAGAAAGATACGGATTCACATTTCCACTGTTGCAAGATCATAAACAAGGTCATCAATTAGTGATAAAAGATGCATTTCTCAATGAAGTCAAAGCCGTGTTTCATGATAAGTTGGAAAAATATTTTGAGTTTTTACAAGTTATCACAAATCACAAGGCTCAAGGAATTGATACGGGAGGTGTTGCGGCAATAGTGAAAGAGTTATTTAAAGACCATACAAATTTAATTTTGGGATTCAATGCCTTGCTGCCAGAGGAACATCAAATCACACTTCCATTTGAGCTTCATTGCCATGAGACTGGTTAG
Protein sequence

>MsaT046518.1

MNSSTSDALFDDAGAYDHAVKSACQEKYSEFAEVMSDYRDGRIDPRGVKARVHELFKGHKHLILGINNIMPKNYEIILPPSDEKLNRQDATTFLKQVKVVFQDKMEKYYEFLQVIQDYMNLTIDILDVIKIAMKLFKGHVDLLSGFNYFLPERYGFTFPLLQDHKQGHQLVIKDAFLNEVKAVFHDKLEKYFEFLQVITNHKAQGIDTGGVAAIVKELFKDHTNLILGFNALLPEEHQITLPFELHCHETG*