AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046543

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046543 MtrunA17_Chr4g0076731 87.150 428 13 6 1 394 15 434 0.0 693
MsaG046543 MtrunA17_Chr6g0453131 52.593 135 37 1 33 140 8 142 4.47e-42 152
MsaG046543 MtrunA17_Chr4g0038751 47.333 150 49 3 39 159 14 162 4.95e-42 149
MsaG046543 MtrunA17_Chr5g0417001 41.872 203 74 6 31 193 5 203 6.30e-42 151
MsaG046543 MtrunA17_Chr8g0361731 46.626 163 46 5 37 158 11 173 1.44e-41 150
MsaG046543 MtrunA17_Chr5g0442771 37.545 277 109 10 25 242 3 274 7.29e-40 144
MsaG046543 MtrunA17_Chr2g0318101 43.713 167 51 4 42 165 17 183 8.30e-38 139
MsaG046543 MtrunA17_Chr6g0481091 47.059 153 47 4 42 161 8 159 1.26e-37 136
MsaG046543 MtrunA17_Chr8g0384721 46.104 154 46 3 42 160 9 160 1.33e-37 137
MsaG046543 MtrunA17_Chr3g0124761 47.500 160 47 5 42 166 9 166 1.44e-37 137
MsaG046543 MtrunA17_Chr8g0359921 48.000 150 44 4 42 158 16 164 1.65e-37 138
MsaG046543 MtrunA17_Chr7g0252971 39.604 202 66 6 42 198 10 200 3.37e-37 137
MsaG046543 MtrunA17_Chr5g0417791 44.311 167 50 5 42 166 5 170 3.80e-37 135
MsaG046543 MtrunA17_Chr2g0318081 46.452 155 51 4 42 165 18 171 4.32e-37 137
MsaG046543 MtrunA17_Chr4g0047841 40.761 184 54 4 37 165 12 195 1.92e-36 135
MsaG046543 MtrunA17_Chr3g0130731 47.059 153 45 4 42 160 9 159 3.53e-36 134
MsaG046543 MtrunA17_Chr2g0326361 38.710 217 84 7 42 210 16 231 8.33e-36 133
MsaG046543 MtrunA17_Chr4g0042561 44.737 152 48 4 42 158 11 161 1.01e-35 132
MsaG046543 MtrunA17_Chr2g0311651 43.646 181 45 6 27 158 14 186 2.78e-35 132
MsaG046543 MtrunA17_Chr2g0309891 43.421 152 51 3 42 159 8 158 7.23e-35 131
MsaG046543 MtrunA17_Chr7g0263931 43.452 168 55 5 31 160 2 167 9.10e-35 130
MsaG046543 MtrunA17_Chr5g0433781 46.405 153 48 4 42 161 8 159 1.59e-34 135
MsaG046543 MtrunA17_Chr2g0311661 43.646 181 44 6 27 158 14 185 1.80e-34 129
MsaG046543 MtrunA17_Chr3g0109821 44.805 154 50 4 42 161 8 160 2.49e-34 134
MsaG046543 MtrunA17_Chr6g0453451 43.791 153 52 4 42 161 22 173 1.35e-33 127
MsaG046543 MtrunA17_Chr2g0309331 42.105 152 56 3 39 158 9 160 1.63e-32 125
MsaG046543 MtrunA17_Chr6g0484441 42.949 156 53 5 42 165 7 158 3.87e-32 124
MsaG046543 MtrunA17_Chr3g0139331 40.625 160 65 4 31 160 2 161 6.66e-32 123
MsaG046543 MtrunA17_Chr2g0316971 49.206 126 36 3 42 139 25 150 7.10e-32 124
MsaG046543 MtrunA17_Chr4g0060931 41.111 180 62 8 25 166 4 177 1.44e-31 122
MsaG046543 MtrunA17_Chr2g0312321 42.012 169 65 4 42 178 13 180 1.59e-31 121
MsaG046543 MtrunA17_Chr5g0428991 45.638 149 47 4 43 158 28 175 1.62e-31 126
MsaG046543 MtrunA17_Chr3g0091921 40.237 169 62 6 25 159 4 167 2.25e-31 121
MsaG046543 MtrunA17_Chr1g0201021 41.176 153 56 3 41 160 20 171 2.47e-31 125
MsaG046543 MtrunA17_Chr4g0056541 41.722 151 55 4 40 158 7 156 2.91e-31 122
MsaG046543 MtrunA17_Chr3g0144271 42.105 152 54 3 42 159 25 176 3.20e-31 123
MsaG046543 MtrunA17_Chr5g0398971 40.789 152 57 3 40 158 7 158 3.64e-31 121
MsaG046543 MtrunA17_Chr4g0018391 42.384 151 54 4 40 158 7 156 8.74e-31 120
MsaG046543 MtrunA17_Chr1g0183741 36.816 201 83 5 42 198 12 212 1.45e-30 118
MsaG046543 MtrunA17_Chr1g0147421 39.877 163 66 5 36 166 23 185 1.58e-30 120
MsaG046543 MtrunA17_Chr5g0400461 43.871 155 47 6 42 158 8 160 2.09e-30 119
MsaG046543 MtrunA17_Chr4g0017921 39.216 153 61 3 40 161 8 159 2.37e-30 119
MsaG046543 MtrunA17_Chr3g0128151 43.624 149 50 4 43 158 25 172 2.38e-30 122
MsaG046543 MtrunA17_Chr3g0128111 36.898 187 74 5 5 158 17 192 1.20e-29 115
MsaG046543 MtrunA17_Chr7g0266701 37.576 165 59 3 38 159 2 165 1.28e-29 115
MsaG046543 MtrunA17_Chr1g0200561 40.132 152 58 3 39 158 18 168 1.71e-29 117
MsaG046543 MtrunA17_Chr8g0346001 38.889 162 55 4 40 158 15 175 2.49e-29 118
MsaG046543 MtrunA17_Chr7g0261411 41.958 143 55 2 42 156 12 154 6.59e-29 114
MsaG046543 MtrunA17_Chr5g0405211 39.490 157 62 4 34 158 2 157 7.45e-29 115
MsaG046543 MtrunA17_Chr6g0461661 45.600 125 40 3 43 139 21 145 1.35e-28 115
MsaG046543 MtrunA17_Chr4g0026141 35.678 199 82 6 37 189 2 200 1.53e-28 112
MsaG046543 MtrunA17_Chr7g0214071 41.667 168 67 6 42 180 17 182 1.69e-28 112
MsaG046543 MtrunA17_Chr1g0197351 40.994 161 55 4 40 161 42 201 2.23e-28 115
MsaG046543 MtrunA17_Chr2g0321631 39.623 159 56 3 42 160 6 164 2.72e-28 111
MsaG046543 MtrunA17_Chr3g0130101 37.975 158 64 4 36 160 3 159 3.59e-28 113
MsaG046543 MtrunA17_Chr7g0253011 44.531 128 41 3 42 139 19 146 4.26e-28 114
MsaG046543 MtrunA17_Chr8g0364791 35.714 154 65 4 40 160 6 158 6.78e-28 114
MsaG046543 MtrunA17_Chr4g0054791 44.079 152 50 5 42 158 8 159 1.34e-27 111
MsaG046543 MtrunA17_Chr8g0372121 41.060 151 56 4 40 158 6 155 1.44e-27 110
MsaG046543 MtrunA17_Chr1g0197361 38.158 152 60 4 42 160 10 160 1.92e-27 114
MsaG046543 MtrunA17_Chr3g0092671 38.816 152 59 4 42 160 7 157 2.08e-27 113
MsaG046543 MtrunA17_Chr7g0217721 44.355 124 41 2 43 139 22 144 7.21e-27 109
MsaG046543 MtrunA17_Chr8g0384201 34.416 154 67 4 40 160 6 158 8.41e-27 111
MsaG046543 MtrunA17_Chr8g0385991 41.772 158 55 5 42 162 16 173 1.27e-26 106
MsaG046543 MtrunA17_Chr7g0217711 43.548 124 42 2 43 139 22 144 2.15e-26 104
MsaG046543 MtrunA17_Chr3g0113001 37.824 193 77 7 58 222 10 187 1.40e-25 105
MsaG046543 MtrunA17_Chr8g0345671 39.355 155 54 7 35 155 7 155 2.37e-23 100
MsaG046543 MtrunA17_Chr7g0243371 57.333 75 31 1 66 139 17 91 7.82e-23 92.8
MsaG046543 MtrunA17_Chr3g0131911 38.272 162 60 5 42 163 16 177 8.01e-23 96.3
MsaG046543 MtrunA17_Chr8g0345691 34.899 149 64 4 38 155 10 156 1.37e-20 92.8
MsaG046543 MtrunA17_Chr2g0321741 54.167 72 32 1 66 137 46 116 6.93e-20 90.1
MsaG046543 MtrunA17_Chr3g0113031 35.795 176 69 7 76 222 3 163 4.33e-18 83.6
MsaG046543 MtrunA17_Chr8g0345711 34.848 132 54 4 37 137 9 139 1.11e-17 80.1
MsaG046543 MtrunA17_Chr8g0345721 42.169 83 46 2 56 137 110 191 1.92e-14 72.0
MsaG046543 MtrunA17_Chr4g0050701 30.323 155 62 4 42 158 13 159 2.49e-14 71.2
MsaG046543 MtrunA17_Chr8g0345741 35.780 109 67 3 59 166 40 146 6.08e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046543 AT2G02450.1 73.457 162 14 2 39 171 50 211 3.41e-78 246
MsaG046543 AT2G02450.2 73.457 162 14 2 39 171 50 211 3.96e-78 247
MsaG046543 AT5G39820.1 52.381 147 39 3 25 140 2 148 3.62e-44 156
MsaG046543 AT2G17040.1 47.682 151 49 3 38 159 4 153 1.34e-41 148
MsaG046543 AT1G26870.1 40.196 204 81 5 29 191 5 208 1.58e-40 149
MsaG046543 AT4G27410.2 48.649 148 44 4 42 158 16 162 3.34e-39 142
MsaG046543 AT3G04070.2 37.165 261 103 9 42 259 12 254 3.39e-39 143
MsaG046543 AT1G61110.1 45.506 178 53 5 37 171 13 189 1.04e-38 141
MsaG046543 AT3G15500.1 49.324 148 43 4 42 158 16 162 2.34e-38 140
MsaG046543 AT2G43000.1 38.021 192 84 4 32 189 10 200 4.51e-38 138
MsaG046543 AT5G08790.1 47.097 155 44 4 43 161 10 162 4.60e-38 139
MsaG046543 AT1G52890.1 49.324 148 43 4 42 158 16 162 5.65e-38 139
MsaG046543 AT3G04070.1 35.018 277 103 9 42 259 12 270 2.12e-37 139
MsaG046543 AT1G69490.1 45.455 154 48 4 42 160 11 163 2.99e-37 136
MsaG046543 AT3G03200.1 45.570 158 51 4 42 165 8 164 1.24e-36 139
MsaG046543 AT1G01720.1 47.097 155 45 3 42 161 9 161 1.55e-36 135
MsaG046543 AT4G27410.3 43.636 165 44 4 42 158 16 179 1.63e-36 135
MsaG046543 AT5G63790.2 46.753 154 44 4 43 160 63 214 2.16e-36 135
MsaG046543 AT5G63790.1 46.753 154 44 4 43 160 53 204 2.31e-36 135
MsaG046543 AT3G17730.1 45.098 153 50 4 42 161 8 159 3.91e-36 132
MsaG046543 AT1G77450.1 46.753 154 46 4 42 161 12 163 5.64e-36 132
MsaG046543 AT5G17260.1 43.671 158 54 3 42 165 8 164 1.15e-35 136
MsaG046543 AT3G10490.1 45.752 153 48 4 40 158 27 178 1.85e-35 130
MsaG046543 AT3G15510.1 42.683 164 51 4 44 164 21 184 2.09e-35 134
MsaG046543 AT1G76420.1 43.333 150 54 3 42 160 24 173 3.11e-35 132
MsaG046543 AT3G10480.2 44.516 155 52 4 40 161 27 180 9.78e-35 133
MsaG046543 AT3G10490.2 44.872 156 51 4 40 161 27 181 1.03e-34 133
MsaG046543 AT5G39610.1 42.593 162 53 4 33 158 13 170 1.19e-34 130
MsaG046543 AT3G29035.1 46.154 143 43 3 27 139 11 149 1.20e-34 130
MsaG046543 AT3G10480.1 44.231 156 52 4 40 161 27 181 1.24e-34 133
MsaG046543 AT3G15170.1 44.805 154 51 4 42 161 22 175 7.52e-34 128
MsaG046543 AT1G65910.1 45.098 153 50 4 42 161 8 159 1.36e-33 132
MsaG046543 AT1G34180.3 41.830 153 55 3 42 161 18 169 1.05e-32 128
MsaG046543 AT2G24430.2 40.373 161 65 4 29 158 5 165 2.11e-32 124
MsaG046543 AT2G24430.1 40.373 161 65 4 29 158 5 165 2.11e-32 124
MsaG046543 AT5G07680.1 43.860 171 54 7 32 165 10 175 3.96e-32 124
MsaG046543 AT1G34180.1 41.830 153 55 3 42 161 18 169 4.27e-32 127
MsaG046543 AT3G04060.1 40.964 166 63 3 27 158 7 171 6.87e-32 124
MsaG046543 AT3G18400.1 43.421 152 47 6 42 159 7 153 8.84e-32 122
MsaG046543 AT5G61430.1 43.137 153 51 5 42 160 18 168 1.51e-31 122
MsaG046543 AT1G34190.1 41.176 153 56 3 42 161 18 169 1.90e-31 125
MsaG046543 AT5G07680.2 44.720 161 48 6 42 165 5 161 2.61e-31 121
MsaG046543 AT1G79580.3 42.667 150 55 3 40 158 17 166 2.78e-31 122
MsaG046543 AT1G79580.5 42.667 150 55 3 40 158 17 166 2.78e-31 122
MsaG046543 AT1G79580.4 42.667 150 55 3 40 158 17 166 2.78e-31 122
MsaG046543 AT1G79580.2 42.667 150 55 3 40 158 17 166 2.78e-31 122
MsaG046543 AT1G79580.1 42.667 150 55 3 40 158 17 166 2.78e-31 122
MsaG046543 AT1G34180.4 38.788 165 55 3 42 161 18 181 5.16e-31 123
MsaG046543 AT1G52880.1 45.223 157 44 5 44 158 21 177 8.64e-31 120
MsaG046543 AT1G33280.1 39.869 153 60 3 40 161 8 159 9.34e-31 120
MsaG046543 AT5G46590.1 43.243 148 50 3 42 155 8 155 9.49e-31 119
MsaG046543 AT1G62700.1 42.384 151 54 4 40 158 7 156 1.09e-30 121
MsaG046543 AT1G62700.2 42.384 151 54 4 40 158 7 156 1.09e-30 121
MsaG046543 AT5G24590.2 37.736 159 66 2 43 168 16 174 1.12e-30 122
MsaG046543 AT1G12260.1 42.384 151 54 4 40 158 7 156 1.58e-30 121
MsaG046543 AT1G34180.2 38.788 165 55 3 42 161 18 181 1.84e-30 123
MsaG046543 AT1G56010.2 42.105 152 52 5 42 158 21 171 1.84e-30 119
MsaG046543 AT1G12260.2 42.384 151 54 4 40 158 7 156 1.98e-30 119
MsaG046543 AT5G18270.2 47.200 125 39 1 42 139 23 147 4.04e-30 119
MsaG046543 AT5G04410.1 39.610 154 59 4 40 160 9 161 4.15e-30 122
MsaG046543 AT5G46590.2 45.455 132 43 2 42 144 8 139 4.26e-30 117
MsaG046543 AT5G18270.1 47.200 125 39 1 42 139 23 147 4.47e-30 119
MsaG046543 AT5G53950.1 47.059 136 42 4 34 139 9 144 7.21e-30 119
MsaG046543 AT4G17980.2 42.484 153 52 4 42 158 8 160 8.17e-30 117
MsaG046543 AT5G13180.1 47.651 149 48 5 42 160 16 164 8.27e-30 115
MsaG046543 AT3G10500.1 38.961 154 60 4 40 160 9 161 9.35e-30 120
MsaG046543 AT4G17980.1 42.484 153 52 4 42 158 8 160 1.01e-29 115
MsaG046543 AT4G10350.1 38.854 157 59 4 40 161 9 163 2.24e-29 117
MsaG046543 AT2G46770.1 36.686 169 59 4 40 161 16 183 2.26e-29 117
MsaG046543 AT3G12977.1 42.763 152 48 5 42 158 20 167 2.61e-29 115
MsaG046543 AT5G62380.2 37.349 166 63 4 42 171 9 169 2.71e-29 117
MsaG046543 AT5G62380.1 37.349 166 63 4 42 171 9 169 2.71e-29 117
MsaG046543 AT3G61910.1 45.455 132 41 3 40 141 11 141 2.90e-29 116
MsaG046543 AT4G35580.3 50.400 125 32 3 43 138 12 135 3.00e-29 119
MsaG046543 AT4G35580.1 50.400 125 32 3 43 138 12 135 3.11e-29 119
MsaG046543 AT4G35580.2 50.400 125 32 3 43 138 12 135 3.20e-29 119
MsaG046543 AT4G36160.1 28.671 286 152 7 40 289 10 279 7.96e-29 116
MsaG046543 AT4G36160.3 28.671 286 152 7 40 289 10 279 7.96e-29 116
MsaG046543 AT1G54330.1 42.177 147 54 3 42 158 8 153 8.88e-29 114
MsaG046543 AT4G36160.2 28.671 286 152 7 40 289 14 283 9.52e-29 115
MsaG046543 AT1G71930.2 40.940 149 55 4 42 158 11 158 1.24e-28 114
MsaG046543 AT1G71930.1 40.940 149 55 4 42 158 11 158 1.24e-28 114
MsaG046543 AT1G33060.2 40.940 149 54 3 43 158 27 174 1.29e-28 118
MsaG046543 AT1G33060.1 40.940 149 54 3 43 158 27 174 1.37e-28 118
MsaG046543 AT1G32510.1 40.462 173 59 5 42 174 8 176 1.38e-28 113
MsaG046543 AT2G33480.2 44.681 141 51 4 42 156 17 156 1.66e-28 112
MsaG046543 AT3G12910.1 38.506 174 61 3 32 159 13 186 1.89e-28 113
MsaG046543 AT2G18060.3 40.397 151 57 4 40 158 9 158 2.76e-28 114
MsaG046543 AT2G18060.1 40.397 151 57 4 40 158 9 158 2.76e-28 114
MsaG046543 AT4G28530.1 38.889 162 55 3 42 159 12 173 2.97e-28 114
MsaG046543 AT1G32770.1 43.077 130 43 2 40 139 16 144 3.65e-28 114
MsaG046543 AT2G33480.1 45.455 143 49 5 42 156 17 158 5.21e-28 111
MsaG046543 AT5G22380.1 39.506 162 59 3 38 160 3 164 8.64e-28 110
MsaG046543 AT3G10480.3 47.788 113 53 2 54 161 103 214 5.99e-27 112
MsaG046543 AT3G49530.2 37.584 149 60 3 43 158 16 164 6.50e-27 111
MsaG046543 AT3G49530.1 37.267 161 64 4 43 166 16 176 8.81e-27 111
MsaG046543 AT3G44350.2 35.294 204 74 8 43 192 8 207 9.03e-27 107
MsaG046543 AT1G56010.1 47.619 105 47 3 61 158 1 104 1.02e-26 107
MsaG046543 AT5G61430.2 49.533 107 45 4 61 160 1 105 1.08e-26 108
MsaG046543 AT1G32870.2 40.000 160 58 5 36 160 6 162 3.26e-26 110
MsaG046543 AT1G32870.1 40.000 160 58 5 36 160 6 162 3.46e-26 110
MsaG046543 AT3G12977.2 48.571 105 42 3 62 158 14 114 4.12e-26 105
MsaG046543 AT1G32870.3 40.000 160 58 5 36 160 42 198 5.99e-26 110
MsaG046543 AT5G66300.1 39.474 152 58 5 40 158 12 162 6.82e-26 106
MsaG046543 AT4G10350.2 43.590 117 56 4 53 161 2 116 2.32e-24 102
MsaG046543 AT3G44350.1 41.406 128 43 3 43 138 8 135 7.01e-24 97.4
MsaG046543 AT5G09330.5 35.065 154 66 4 40 160 6 158 1.26e-23 102
MsaG046543 AT5G09330.2 35.065 154 66 4 40 160 6 158 1.26e-23 102
MsaG046543 AT5G09330.4 35.065 154 66 4 40 160 6 158 1.26e-23 102
MsaG046543 AT5G09330.3 35.065 154 66 4 40 160 6 158 1.26e-23 102
MsaG046543 AT5G09330.1 35.065 154 66 4 40 160 6 158 1.26e-23 102
MsaG046543 AT2G27300.1 37.086 151 57 5 40 159 14 157 1.86e-23 100
MsaG046543 AT2G43000.2 40.984 122 64 3 75 189 2 122 3.89e-23 96.3
MsaG046543 AT5G64060.1 29.876 241 118 7 40 247 6 228 4.82e-23 99.8
MsaG046543 AT5G22290.1 35.185 162 74 4 22 155 3 161 1.46e-22 97.8
MsaG046543 AT2G33480.3 50.526 95 42 3 66 156 8 101 4.09e-22 94.0
MsaG046543 AT5G04395.1 35.417 144 51 3 42 143 30 173 5.06e-21 90.1
MsaG046543 AT3G44290.2 38.400 125 48 3 41 137 15 138 2.18e-20 89.7
MsaG046543 AT4G01550.1 34.161 161 67 5 41 166 5 161 5.12e-20 92.0
MsaG046543 AT3G44290.1 38.400 125 48 3 41 137 15 138 6.93e-20 90.1
MsaG046543 AT3G10490.4 50.617 81 34 2 86 161 2 81 1.10e-19 89.7
MsaG046543 AT3G10490.3 50.617 81 34 2 86 161 2 81 1.10e-19 89.7
MsaG046543 AT4G01540.3 35.849 159 61 6 41 162 5 159 1.31e-18 87.4
MsaG046543 AT4G01520.1 35.669 157 60 6 43 162 7 159 1.63e-18 85.9
MsaG046543 AT4G01540.4 36.306 157 59 6 43 162 7 159 1.86e-18 86.7
MsaG046543 AT4G01540.1 36.306 157 59 6 43 162 7 159 2.23e-18 87.0
MsaG046543 AT4G01540.2 36.306 157 59 6 43 162 7 159 2.81e-18 86.7
MsaG046543 AT3G04420.2 30.263 152 73 3 43 163 6 155 6.92e-15 75.1
MsaG046543 AT3G04420.1 30.263 152 73 3 43 163 6 155 1.25e-14 74.7
MsaG046543 AT1G02250.1 31.507 146 64 4 43 155 6 148 3.53e-14 73.6
MsaG046543 AT5G64530.2 35.433 127 42 5 42 140 5 119 2.51e-13 67.8
MsaG046543 AT4G28530.2 35.156 128 35 3 42 123 12 137 3.88e-13 70.1
MsaG046543 AT1G02230.1 30.822 146 65 4 43 155 6 148 4.40e-13 70.5
MsaG046543 AT2G18060.2 42.857 70 37 2 91 158 2 70 1.10e-12 68.2
MsaG046543 AT1G02220.1 29.605 152 71 4 43 163 6 152 5.65e-12 67.4

Find 103 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTAACAACTTCTGGTTAT+TGG 0.211019 8:+85672232 MsaT046543.1:CDS
GATGATGATGGTCGTGTAAT+TGG 0.239615 8:-85673265 None:intergenic
TAAGCAGCAAAATGAAATAT+TGG 0.264868 8:+85673324 MsaT046543.1:CDS
ACTGAAAACTTTCGTTCAAT+TGG 0.296485 8:+85672285 MsaT046543.1:CDS
CCTAATCGTGTAACAACTTC+TGG 0.308486 8:+85672225 MsaT046543.1:CDS
CCAGAAGTTGTTACACGATT+AGG 0.317297 8:-85672225 None:intergenic
ATATCGTTCGGTTTCATGTT+GGG 0.335622 8:-85672391 None:intergenic
AAGGAGGTTGGAAATTGATT+TGG 0.344511 8:-85673832 None:intergenic
TGAAGCTGAGACTGGGAAAA+TGG 0.347823 8:-85673635 None:intergenic
GCTGCCGCTGAAGCTGAGAC+TGG 0.355613 8:-85673643 None:intergenic
GTAGACAAATGATTGGGATT+TGG 0.373594 8:-85673787 None:intergenic
CAACTGGTTCGGATGCCTTT+GGG 0.374805 8:-85672345 None:intergenic
CCAACTGGTTCGGATGCCTT+TGG 0.377419 8:-85672346 None:intergenic
AAGCAGCAAAATGAAATATT+GGG 0.379687 8:+85673325 MsaT046543.1:CDS
TCTTCTGAACGGTAATTAGT+AGG 0.410368 8:-85673487 None:intergenic
GATCATGTCACAACCACTCT+TGG 0.411521 8:+85673436 MsaT046543.1:CDS
ATGTGATCATCAATTGTCTT+TGG 0.414276 8:-85673373 None:intergenic
ATGATGATGGTCGTGTAATT+GGG 0.415001 8:-85673264 None:intergenic
GAGGTGTTGTTACTACTGTT+GGG 0.428986 8:-85673904 None:intergenic
TGCAGCACTGGTAGCAGTAT+TGG 0.434071 8:-85673680 None:intergenic
TTGGGGTGATTATGATCCTC+AGG 0.436862 8:-85673886 None:intergenic
CAGCAATGTAGACAAATGAT+TGG 0.439526 8:-85673794 None:intergenic
TCTGGTTATTGGAAAGCAAC+AGG 0.440994 8:+85672243 MsaT046543.1:CDS
GATATCGTTCGGTTTCATGT+TGG 0.441960 8:-85672392 None:intergenic
TTGGGAATGGGATTTGTTGT+TGG 0.446359 8:+85673343 MsaT046543.1:CDS
TGATGATGGATGATCTCAAT+AGG 0.454191 8:+85673704 MsaT046543.1:CDS
GCAAAATGAAATATTGGGAA+TGG 0.458597 8:+85673330 MsaT046543.1:CDS
GGAGGTGTTGTTACTACTGT+TGG 0.465590 8:-85673905 None:intergenic
CAAAATGAAATATTGGGAAT+GGG 0.469377 8:+85673331 MsaT046543.1:CDS
GAGCATGACGTGGTTATGCC+AGG 0.470442 8:+85671661 MsaT046543.1:CDS
TCTCTGACCGACTGTGGGAC+TGG 0.472209 8:+85673854 MsaT046543.1:CDS
TGATTATGATCCTCAGGGAT+TGG 0.472665 8:-85673880 None:intergenic
ATACTCATTCATAATCCAAC+TGG 0.474720 8:-85672361 None:intergenic
CATTCATAATCCAACTGGTT+CGG 0.480582 8:-85672356 None:intergenic
AGGAGGAGTATTGCTGTACT+TGG 0.484755 8:-85673464 None:intergenic
CAAGCTCTTCTTCAGTTGGA+TGG 0.486659 8:-85671693 None:intergenic
AGTAATTAGTGATTACTTGA+AGG 0.490931 8:-85673926 None:intergenic
ATCATGTCACAACCACTCTT+GGG 0.491534 8:+85673437 MsaT046543.1:CDS
GGCGCTGCCGCCACCACAAG+CGG 0.493139 8:-85673614 None:intergenic
GATTTGGATGATTGAGATGT+GGG 0.497103 8:-85673771 None:intergenic
TTCCCTCGACCTTACGGCGA+AGG 0.497938 8:-85671726 None:intergenic
CTGCCGCTGAAGCTGAGACT+GGG 0.499254 8:-85673642 None:intergenic
GGAAATTGATTTGGTAACAT+TGG 0.503521 8:-85673823 None:intergenic
TATCGTTCGGTTTCATGTTG+GGG 0.505358 8:-85672390 None:intergenic
TTAGTCTCCGCTTGTGGTGG+CGG 0.506486 8:+85673607 MsaT046543.1:CDS
TCTACAAGCTCTTCTTCAGT+TGG 0.506937 8:-85671697 None:intergenic
TGAAACCGAACGATATCAAA+AGG 0.509230 8:+85672398 MsaT046543.1:CDS
ATCCATCATCATTGCAGCAC+TGG 0.512112 8:-85673692 None:intergenic
TTTGGCAGCAATCGGAGAGA+AGG 0.516877 8:+85672152 MsaT046543.1:CDS
GGAAAGCAACAGGAGCAGAT+AGG 0.517048 8:+85672253 MsaT046543.1:CDS
AGCAATGTAGACAAATGATT+GGG 0.519527 8:-85673793 None:intergenic
TGAGCAGCTTTGGCAGCAAT+CGG 0.520334 8:+85672144 MsaT046543.1:intron
GGGTGAGATATCCCTATGTC+GGG 0.523444 8:+85673192 MsaT046543.1:intron
TACCGTTCAGAAGATGAAGA+AGG 0.528026 8:+85673496 MsaT046543.1:CDS
CCTCCTTCTCTGACCGACTG+TGG 0.530397 8:+85673848 MsaT046543.1:CDS
CACATAGTTAACAGAATGGA+AGG 0.538689 8:+85673391 MsaT046543.1:CDS
CCACAGTCGGTCAGAGAAGG+AGG 0.546229 8:-85673848 None:intergenic
TGGGAGACAACGAGGGAGGG+AGG 0.546963 8:-85673245 None:intergenic
GCATATTGTACTAGTTGCCT+AGG 0.547334 8:-85673565 None:intergenic
GTCCCACAGTCGGTCAGAGA+AGG 0.552251 8:-85673851 None:intergenic
GGGAGACAACGAGGGAGGGA+GGG 0.560158 8:-85673244 None:intergenic
AGTCGGTCAGAGAAGGAGGT+TGG 0.561135 8:-85673844 None:intergenic
TACCAGTGCTGCAATGATGA+TGG 0.562350 8:+85673690 MsaT046543.1:CDS
CTCCTTCTCTGACCGACTGT+GGG 0.564074 8:+85673849 MsaT046543.1:CDS
CTAGTACAATATGCAGCTGC+GGG 0.565924 8:+85673574 MsaT046543.1:CDS
ATCCTTCTTCATCTTCTGAA+CGG 0.566170 8:-85673498 None:intergenic
TGGGGTGATTATGATCCTCA+GGG 0.566445 8:-85673885 None:intergenic
GAACGGTAATTAGTAGGAGG+AGG 0.572179 8:-85673481 None:intergenic
AGGGTGAGATATCCCTATGT+CGG 0.573960 8:+85673191 MsaT046543.1:intron
GGATTTGGATGATTGAGATG+TGG 0.580035 8:-85673772 None:intergenic
AATTAGTGATTACTTGAAGG+AGG 0.582479 8:-85673923 None:intergenic
ATGAGCTTGAGTCACGAAGA+TGG 0.587554 8:+85671556 MsaT046543.1:CDS
CTGTTAGTCTCCGCTTGTGG+TGG 0.588546 8:+85673604 MsaT046543.1:CDS
TCTCTTGTATACCCGACATA+GGG 0.588580 8:-85673203 None:intergenic
CTCTCTTGTATACCCGACAT+AGG 0.590093 8:-85673204 None:intergenic
CCAAAGGCATCCGAACCAGT+TGG 0.592737 8:+85672346 MsaT046543.1:CDS
CGAGATAGAAAGTATCGAAA+CGG 0.593685 8:+85672195 MsaT046543.1:CDS
TGATCACATAGTTAACAGAA+TGG 0.595016 8:+85673387 MsaT046543.1:CDS
AGCATGACGTGGTTATGCCA+GGG 0.596795 8:+85671662 MsaT046543.1:CDS
TGTCGGGTATACAAGAGAGC+AGG 0.602519 8:+85673208 MsaT046543.1:CDS
AATTGGGAGACAACGAGGGA+GGG 0.605068 8:-85673248 None:intergenic
TTTCGATACTTTCTATCTCG+AGG 0.612931 8:-85672192 None:intergenic
CGACCATCATCATCATCAAG+AGG 0.614625 8:+85673274 MsaT046543.1:CDS
TCTAACGATAATAGTAACAA+AGG 0.615780 8:+85671592 MsaT046543.1:CDS
TCTCTGTTAGTCTCCGCTTG+TGG 0.618457 8:+85673601 MsaT046543.1:CDS
TTTCCCAGTCTCAGCTTCAG+CGG 0.622682 8:+85673639 MsaT046543.1:CDS
TCTGAACGGTAATTAGTAGG+AGG 0.623921 8:-85673484 None:intergenic
ACTAGTACAATATGCAGCTG+CGG 0.627166 8:+85673573 MsaT046543.1:CDS
GTACCTCTTGATGATGATGA+TGG 0.633918 8:-85673277 None:intergenic
AGGTGTTGTTACTACTGTTG+GGG 0.635388 8:-85673903 None:intergenic
AGAATGGAAGGAAGCAGTAG+CGG 0.638297 8:+85673403 MsaT046543.1:CDS
CAGCAATCGGAGAGAAGGAG+TGG 0.646393 8:+85672157 MsaT046543.1:CDS
AGCAGCAGCAACATCAACCT+AGG 0.647554 8:+85673548 MsaT046543.1:CDS
TATTCTGGAAAAGCTCCCAA+AGG 0.650827 8:+85672330 MsaT046543.1:CDS
GTTGGATGGAAACGAAACCC+TGG 0.652572 8:-85671679 None:intergenic
TAATTGGGAGACAACGAGGG+AGG 0.654012 8:-85673249 None:intergenic
CGTGTAATTGGGAGACAACG+AGG 0.654137 8:-85673253 None:intergenic
TTGGATTCCAGTCCCACAGT+CGG 0.664258 8:-85673861 None:intergenic
ATGGAAGGAAGCAGTAGCGG+CGG 0.680492 8:+85673406 MsaT046543.1:CDS
GTACTTGGAAAGCCCAAGAG+TGG 0.699274 8:-85673449 None:intergenic
GTGTAATTGGGAGACAACGA+GGG 0.701719 8:-85673252 None:intergenic
TGATGATCACGAGCATGACG+TGG 0.732266 8:+85671651 MsaT046543.1:CDS
GGACTGGAATCCAATCCCTG+AGG 0.753349 8:+85673870 MsaT046543.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 85671556 85673936 85671556 ID=MsaG046543
Chr8 mRNA 85671556 85673936 85671556 ID=MsaT046543.1;Parent=MsaG046543
Chr8 exon 85671556 85671733 85671556 ID=MsaT046543.1.exon1;Parent=MsaT046543.1
Chr8 CDS 85671556 85671733 85671556 ID=cds.MsaT046543.1;Parent=MsaT046543.1
Chr8 exon 85672151 85672419 85672151 ID=MsaT046543.1.exon2;Parent=MsaT046543.1
Chr8 CDS 85672151 85672419 85672151 ID=cds.MsaT046543.1;Parent=MsaT046543.1
Chr8 exon 85673199 85673936 85673199 ID=MsaT046543.1.exon3;Parent=MsaT046543.1
Chr8 CDS 85673199 85673936 85673199 ID=cds.MsaT046543.1;Parent=MsaT046543.1
Gene Sequence

>MsaT046543.1

ATGAGCTTGAGTCACGAAGATGGAACTGCTTCTACTTCTAACGATAATAGTAACAAAGGAACTACAGCTACAGTTACAGTAGTAGATGATGATGATGATGATCACGAGCATGACGTGGTTATGCCAGGGTTTCGTTTCCATCCAACTGAAGAAGAGCTTGTAGAGTTCTACCTTCGCCCTTTGGCAGCAATCGGAGAGAAGGAGTGGTACTTTTATGTGCCTCGAGATAGAAAGTATCGAAACGGTGATCGTCCTAATCGTGTAACAACTTCTGGTTATTGGAAAGCAACAGGAGCAGATAGGATGATTCGAACTGAAAACTTTCGTTCAATTGGACTCAAGAAAACCCTAGTTTTCTATTCTGGAAAAGCTCCCAAAGGCATCCGAACCAGTTGGATTATGAATGAGTATAGATTACCCCAACATGAAACCGAACGATATCAAAAGATATCCCTATGTCGGGTATACAAGAGAGCAGGAGTAGAAGATCATCCCTCCCTCCCTCGTTGTCTCCCAATTACACGACCATCATCATCATCAAGAGGTACTACTGCTTCACATTCAGACAAGAATAAGCAGCAAAATGAAATATTGGGAATGGGATTTGTTGTTGGACAATCACCAAAGACAATTGATGATCACATAGTTAACAGAATGGAAGGAAGCAGTAGCGGCGGTAATTCAGATCATGTCACAACCACTCTTGGGCTTTCCAAGTACAGCAATACTCCTCCTCCTACTAATTACCGTTCAGAAGATGAAGAAGGATTGATGATGATGCAGCAGCAGCAGCAGCAGCAGCAGCAACATCAACCTAGGCAACTAGTACAATATGCAGCTGCGGGAAACTCTCTGTTAGTCTCCGCTTGTGGTGGCGGCAGCGCCATTTTCCCAGTCTCAGCTTCAGCGGCAGCAGCAGTGAGCTGTTCCAATACTGCTACCAGTGCTGCAATGATGATGGATGATCTCAATAGGCTTGTAAGTTATCAACAACAGCAATATTGTTACAATGTTCAAAACCCACATCTCAATCATCCAAATCCCAATCATTTGTCTACATTGCTGATGCAACCAATGTTACCAAATCAATTTCCAACCTCCTTCTCTGACCGACTGTGGGACTGGAATCCAATCCCTGAGGATCATAATCACCCCAACAGTAGTAACAACACCTCCTTCAAGTAA
Protein sequence

>MsaT046543.1

MSLSHEDGTASTSNDNSNKGTTATVTVVDDDDDDHEHDVVMPGFRFHPTEEELVEFYLRPLAAIGEKEWYFYVPRDRKYRNGDRPNRVTTSGYWKATGADRMIRTENFRSIGLKKTLVFYSGKAPKGIRTSWIMNEYRLPQHETERYQKISLCRVYKRAGVEDHPSLPRCLPITRPSSSSRGTTASHSDKNKQQNEILGMGFVVGQSPKTIDDHIVNRMEGSSSGGNSDHVTTTLGLSKYSNTPPPTNYRSEDEEGLMMMQQQQQQQQQHQPRQLVQYAAAGNSLLVSACGGGSAIFPVSASAAAAVSCSNTATSAAMMMDDLNRLVSYQQQQYCYNVQNPHLNHPNPNHLSTLLMQPMLPNQFPTSFSDRLWDWNPIPEDHNHPNSSNNTSFK*