AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046594

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046594 MtrunA17_Chr1g0191981 97.500 120 3 0 1 120 1 120 2.51e-87 249
MsaG046594 MtrunA17_Chr1g0195851 72.632 95 26 0 4 98 36 130 1.18e-49 156
MsaG046594 MtrunA17_Chr2g0296321 68.687 99 31 0 4 102 66 164 5.23e-46 149
MsaG046594 MtrunA17_Chr4g0067091 72.826 92 25 0 4 95 22 113 4.59e-45 145
MsaG046594 MtrunA17_Chr4g0076321 55.752 113 44 1 6 112 21 133 1.55e-41 134
MsaG046594 MtrunA17_Chr2g0290491 63.043 92 34 0 4 95 17 108 1.42e-39 130
MsaG046594 MtrunA17_Chr3g0102351 60.870 92 36 0 4 95 27 118 1.25e-38 128
MsaG046594 MtrunA17_Chr8g0384451 59.794 97 39 0 4 100 40 136 1.54e-38 129
MsaG046594 MtrunA17_Chr5g0446491 60.870 92 36 0 4 95 23 114 3.41e-38 127
MsaG046594 MtrunA17_Chr8g0382931 60.870 92 36 0 4 95 28 119 1.08e-37 126
MsaG046594 MtrunA17_Chr7g0263601 45.455 121 66 0 4 124 26 146 8.76e-37 123
MsaG046594 MtrunA17_Chr1g0185911 55.435 92 41 0 4 95 29 120 7.13e-36 121
MsaG046594 MtrunA17_Chr4g0076381 51.087 92 45 0 4 95 58 149 3.57e-33 116
MsaG046594 MtrunA17_Chr1g0165041 51.087 92 45 0 4 95 6 97 2.21e-31 110
MsaG046594 MtrunA17_Chr5g0446601 44.330 97 54 0 1 97 21 117 5.29e-23 88.6
MsaG046594 MtrunA17_Chr1g0159291 35.000 80 52 0 9 88 7 86 2.31e-17 71.6
MsaG046594 MtrunA17_Chr2g0307551 35.443 79 51 0 6 84 13 91 5.14e-16 69.7
MsaG046594 MtrunA17_Chr1g0159271 38.462 78 48 0 18 95 1 78 1.32e-15 67.4
MsaG046594 MtrunA17_Chr4g0062271 31.646 79 54 0 6 84 13 91 1.04e-13 63.9
MsaG046594 MtrunA17_Chr1g0158951 42.105 57 33 0 39 95 24 80 1.75e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046594 AT1G09030.1 71.845 103 28 1 1 103 1 102 1.11e-50 157
MsaG046594 AT2G47810.1 68.421 95 30 0 1 95 48 142 2.59e-45 145
MsaG046594 AT5G47640.1 64.130 92 33 0 4 95 27 118 3.74e-40 132
MsaG046594 AT4G14540.1 60.870 92 36 0 4 95 21 112 4.71e-39 129
MsaG046594 AT2G13570.1 59.000 100 41 0 4 103 36 135 1.65e-38 129
MsaG046594 AT2G38880.3 50.407 123 55 2 4 126 21 137 3.93e-38 126
MsaG046594 AT2G38880.2 50.407 123 55 2 4 126 21 137 4.98e-38 125
MsaG046594 AT2G38880.1 50.407 123 55 2 4 126 21 137 4.98e-38 125
MsaG046594 AT2G38880.5 50.407 123 55 2 4 126 21 137 4.98e-38 125
MsaG046594 AT2G38880.7 49.593 123 54 2 4 126 21 135 1.19e-37 125
MsaG046594 AT2G38880.4 58.242 91 38 0 4 94 21 111 1.73e-37 123
MsaG046594 AT2G37060.2 56.522 92 40 0 4 95 30 121 5.97e-37 124
MsaG046594 AT2G37060.3 56.522 92 40 0 4 95 30 121 5.97e-37 124
MsaG046594 AT2G37060.1 56.522 92 40 0 4 95 30 121 5.97e-37 124
MsaG046594 AT3G53340.3 55.914 93 41 0 4 96 29 121 5.98e-37 123
MsaG046594 AT3G53340.2 55.914 93 41 0 4 96 29 121 5.98e-37 123
MsaG046594 AT3G53340.4 56.044 91 40 0 4 94 29 119 6.27e-37 122
MsaG046594 AT3G53340.5 56.522 92 40 0 4 95 29 120 8.44e-37 124
MsaG046594 AT3G53340.1 56.522 92 40 0 4 95 29 120 8.44e-37 124
MsaG046594 AT2G38880.11 58.242 91 38 0 4 94 21 111 9.80e-37 123
MsaG046594 AT2G38880.8 58.242 91 38 0 4 94 21 111 9.80e-37 123
MsaG046594 AT2G38880.9 58.242 91 38 0 4 94 21 111 1.10e-36 122
MsaG046594 AT2G38880.10 58.242 91 38 0 4 94 21 111 1.10e-36 122
MsaG046594 AT2G38880.6 58.242 91 38 0 4 94 21 111 1.10e-36 122
MsaG046594 AT5G47670.2 51.087 92 45 0 4 95 29 120 1.99e-32 113
MsaG046594 AT5G47670.1 51.087 92 45 0 4 95 58 149 5.31e-32 113
MsaG046594 AT5G47670.3 51.087 92 45 0 4 95 58 149 5.31e-32 113
MsaG046594 AT1G21970.1 49.474 95 48 0 4 98 59 153 5.88e-31 110
MsaG046594 AT5G23090.5 36.709 79 50 0 6 84 13 91 2.24e-16 71.2
MsaG046594 AT5G23090.2 36.709 79 50 0 6 84 13 91 2.24e-16 71.2
MsaG046594 AT5G23090.1 36.709 79 50 0 6 84 13 91 2.24e-16 71.2
MsaG046594 AT5G23090.4 36.709 79 50 0 6 84 13 91 2.26e-16 71.2
MsaG046594 AT5G08190.1 32.911 79 53 0 6 84 13 91 1.88e-14 66.2
MsaG046594 AT5G08190.2 32.911 79 53 0 6 84 13 91 1.94e-14 66.2

Find 0 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Contig1 gene 116718 117318 116718 ID=MsaG046594
Contig1 mRNA 116718 117318 116718 ID=MsaT046594.1;Parent=MsaG046594
Contig1 exon 116718 116746 116718 ID=MsaT046594.1.exon2;Parent=MsaT046594.1
Contig1 CDS 116718 116746 116718 ID=cds.MsaT046594.1;Parent=MsaT046594.1
Contig1 exon 116961 117318 116961 ID=MsaT046594.1.exon1;Parent=MsaT046594.1
Contig1 CDS 116961 117318 116961 ID=cds.MsaT046594.1;Parent=MsaT046594.1
Gene Sequence

>MsaT046594.1

ATGAACGATGAGGGAGATAAAACGTTGCCCATAGCGAATGTGGGTAGAATAATGAAGCAAAATCTTCCTCCCACTGCAAAGATCTCAAAAGAAAGCAAACAGTTGATGCAAGAATGTGCGACAGAGTTCATAAGTTTCGTGACAGGTGAAGCATCTGACAAGTGTCACAAGGAGAATCGCAAGACAGTTAATGGGGATGACATCTGTTGGGCTTTGTGTTCGTTAGGGTTTGACAACTATGCTGAAGCTATTGGAAGGTACTTGTATAAATTCAGACAAGCTGAACTTGTTAGAATAAATCAGAACAAACTTCATGAAACTGCAAAAGACAAGTTTGAAGAAGATGCAACAAATCCAAATGGAATCAATGAAGAAGAACATTTCTAA
Protein sequence

>MsaT046594.1

MNDEGDKTLPIANVGRIMKQNLPPTAKISKESKQLMQECATEFISFVTGEASDKCHKENRKTVNGDDICWALCSLGFDNYAEAIGRYLYKFRQAELVRINQNKLHETAKDKFEEDATNPNGINEEEHF*