AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046595

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046595 MtrunA17_Chr1g0191991 98.182 495 6 2 1 492 1 495 0.0 1001
MsaG046595 MtrunA17_Chr7g0268961 53.721 430 163 12 71 480 32 445 3.73e-134 397
MsaG046595 MtrunA17_Chr2g0330171 60.841 309 89 5 49 347 34 320 1.42e-122 366
MsaG046595 MtrunA17_Chr8g0342421 61.238 307 88 6 59 348 60 352 9.57e-119 358
MsaG046595 MtrunA17_Chr1g0185121 57.225 346 107 11 61 387 40 363 4.65e-118 354
MsaG046595 MtrunA17_Chr8g0373541 73.364 214 53 1 41 250 9 222 2.08e-116 352
MsaG046595 MtrunA17_Chr4g0040571 51.752 371 101 10 58 350 36 406 5.05e-114 345
MsaG046595 MtrunA17_Chr7g0263941 55.821 335 116 5 30 357 3 312 1.15e-113 341
MsaG046595 MtrunA17_Chr4g0029521 44.396 455 187 15 59 479 36 458 4.09e-109 333
MsaG046595 MtrunA17_Chr1g0162971 51.190 336 103 6 61 346 41 365 3.68e-107 326
MsaG046595 MtrunA17_Chr1g0181381 80.925 173 32 1 61 232 38 210 1.13e-106 327
MsaG046595 MtrunA17_Chr1g0151351 80.702 171 32 1 52 221 32 202 6.41e-106 323
MsaG046595 MtrunA17_Chr1g0199101 83.333 162 27 0 59 220 45 206 1.04e-105 325
MsaG046595 MtrunA17_Chr1g0163001 74.866 187 45 1 39 223 19 205 3.55e-105 323
MsaG046595 MtrunA17_Chr1g0210821 84.076 157 25 0 64 220 47 203 1.67e-103 321
MsaG046595 MtrunA17_Chr2g0326381 68.116 207 60 4 60 260 31 237 1.80e-101 310
MsaG046595 MtrunA17_Chr3g0141431 78.882 161 33 1 58 217 19 179 4.30e-97 299
MsaG046595 MtrunA17_Chr2g0324991 90.076 131 13 0 72 202 3 133 2.59e-88 267
MsaG046595 MtrunA17_Chr6g0479921 46.043 278 125 6 61 335 29 284 1.48e-74 243
MsaG046595 MtrunA17_Chr8g0375521 64.744 156 53 2 61 214 27 182 3.50e-72 235
MsaG046595 MtrunA17_Chr1g0200421 33.929 168 95 4 73 224 234 401 1.05e-23 103
MsaG046595 MtrunA17_Chr2g0320341 29.126 206 131 4 19 210 174 378 1.32e-23 102
MsaG046595 MtrunA17_Chr3g0123941 33.696 184 98 4 71 230 118 301 4.27e-23 100
MsaG046595 MtrunA17_Chr8g0352011 35.106 188 96 8 57 226 152 331 2.83e-21 95.1
MsaG046595 MtrunA17_Chr8g0382121 35.811 148 84 5 74 210 109 256 7.29e-21 93.2
MsaG046595 MtrunA17_Chr7g0258011 35.404 161 84 7 84 226 183 341 8.97e-21 93.6
MsaG046595 MtrunA17_Chr5g0422571 32.787 183 110 6 55 226 79 259 4.87e-20 90.5
MsaG046595 MtrunA17_Chr1g0198651 33.520 179 90 8 72 229 103 273 4.12e-19 87.8
MsaG046595 MtrunA17_Chr1g0173841 34.104 173 88 8 72 226 216 380 4.14e-19 89.4
MsaG046595 MtrunA17_Chr2g0328921 35.542 166 87 8 58 213 127 282 2.27e-18 85.9
MsaG046595 MtrunA17_Chr4g0053341 33.113 151 91 4 72 213 69 218 6.61e-18 82.8
MsaG046595 MtrunA17_Chr4g0026521 36.429 140 77 6 84 213 125 262 8.87e-18 84.0
MsaG046595 MtrunA17_Chr2g0321291 36.054 147 82 6 77 213 133 277 1.14e-17 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046595 AT3G50700.1 58.044 317 99 5 61 349 34 344 1.01e-116 351
MsaG046595 AT5G44160.1 56.698 321 106 7 56 345 31 349 2.46e-114 345
MsaG046595 AT1G03840.1 88.304 171 19 1 56 225 35 205 1.11e-113 345
MsaG046595 AT1G03840.2 87.135 171 19 2 56 225 35 203 4.67e-110 336
MsaG046595 AT2G02080.1 41.719 477 174 14 58 480 51 477 1.28e-106 327
MsaG046595 AT2G02080.4 41.719 477 174 14 58 480 51 477 1.28e-106 327
MsaG046595 AT2G02080.5 41.719 477 174 14 58 480 51 477 1.28e-106 327
MsaG046595 AT2G02080.3 41.719 477 174 14 58 480 51 477 1.28e-106 327
MsaG046595 AT1G55110.3 74.742 194 39 2 41 224 34 227 3.33e-106 324
MsaG046595 AT1G55110.1 74.742 194 39 2 41 224 34 227 3.33e-106 324
MsaG046595 AT1G55110.2 74.742 194 39 2 41 224 34 227 3.33e-106 324
MsaG046595 AT1G14580.1 79.412 170 35 0 54 223 46 215 3.70e-106 325
MsaG046595 AT1G14580.2 79.412 170 35 0 54 223 46 215 3.70e-106 325
MsaG046595 AT1G14580.3 79.412 170 35 0 54 223 60 229 7.47e-106 324
MsaG046595 AT5G44160.2 55.410 305 104 6 71 345 8 310 3.40e-105 321
MsaG046595 AT3G13810.4 85.294 170 22 2 61 229 70 237 4.49e-105 323
MsaG046595 AT3G13810.1 85.294 170 22 2 61 229 70 237 4.49e-105 323
MsaG046595 AT3G13810.6 85.294 170 22 2 61 229 51 218 4.55e-105 323
MsaG046595 AT3G13810.5 85.294 170 22 2 61 229 65 232 5.13e-105 323
MsaG046595 AT4G02670.2 77.473 182 39 2 52 232 47 227 6.59e-105 319
MsaG046595 AT3G45260.2 83.537 164 26 1 61 223 39 202 6.72e-105 321
MsaG046595 AT3G45260.1 83.537 164 26 1 61 223 39 202 6.72e-105 321
MsaG046595 AT4G02670.1 77.473 182 39 2 52 232 45 225 7.04e-105 319
MsaG046595 AT5G60470.2 81.098 164 30 1 61 223 44 207 1.14e-101 313
MsaG046595 AT5G03150.1 84.472 161 24 1 61 220 53 213 1.36e-101 314
MsaG046595 AT5G60470.3 81.098 164 30 1 61 223 40 203 1.39e-101 312
MsaG046595 AT2G02070.2 80.368 163 32 0 61 223 52 214 2.28e-101 317
MsaG046595 AT2G02070.1 80.368 163 32 0 61 223 52 214 2.28e-101 317
MsaG046595 AT3G13810.3 85.535 159 20 2 72 229 68 224 2.69e-97 303
MsaG046595 AT3G13810.2 85.535 159 20 2 72 229 82 238 3.51e-97 303
MsaG046595 AT5G60470.1 74.566 173 39 2 56 223 26 198 2.97e-95 296
MsaG046595 AT2G02080.6 39.778 450 167 14 85 480 1 400 9.17e-91 284
MsaG046595 AT2G02080.2 39.778 450 167 14 85 480 1 400 9.17e-91 284
MsaG046595 AT1G68130.1 66.026 156 51 2 61 214 41 196 8.78e-74 239
MsaG046595 AT2G01940.1 61.538 169 63 2 50 216 33 201 2.05e-72 236
MsaG046595 AT1G25250.3 63.694 157 54 2 61 214 33 189 9.10e-72 233
MsaG046595 AT2G01940.3 60.588 170 64 3 50 216 33 202 2.00e-68 226
MsaG046595 AT1G25250.1 64.626 147 49 2 71 214 20 166 6.34e-68 222
MsaG046595 AT1G68130.2 65.455 110 36 2 107 214 1 110 7.46e-47 166
MsaG046595 AT2G01940.2 63.393 112 39 2 107 216 1 112 1.51e-45 163
MsaG046595 AT1G25250.2 62.162 111 39 2 107 214 1 111 1.23e-44 159
MsaG046595 AT5G22890.1 31.667 180 106 5 46 210 174 351 3.16e-23 101
MsaG046595 AT1G34370.3 33.544 158 91 3 70 213 14 171 5.74e-23 99.4
MsaG046595 AT1G34370.2 33.544 158 91 3 70 213 224 381 1.02e-22 101
MsaG046595 AT1G34370.1 33.544 158 91 3 70 213 224 381 1.02e-22 101
MsaG046595 AT1G13290.1 34.591 159 84 7 72 218 89 239 9.87e-19 87.4
MsaG046595 AT3G20880.1 33.125 160 91 5 84 228 250 408 1.38e-18 88.2
MsaG046595 AT3G57670.1 32.370 173 91 7 72 226 205 369 1.70e-18 87.8
MsaG046595 AT1G51220.1 33.113 151 85 5 72 213 166 309 4.45e-18 85.9
MsaG046595 AT1G08290.1 35.294 153 80 7 72 213 168 312 5.41e-17 82.8
MsaG046595 AT1G34790.1 32.667 150 89 5 74 213 129 276 5.57e-16 79.0

Find 0 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Contig1 gene 138990 142808 138990 ID=MsaG046595
Contig1 mRNA 138990 142808 138990 ID=MsaT046595.1;Parent=MsaG046595
Contig1 exon 138990 139200 138990 ID=MsaT046595.1.exon1;Parent=MsaT046595.1
Contig1 CDS 138990 139200 138990 ID=cds.MsaT046595.1;Parent=MsaT046595.1
Contig1 exon 140009 140405 140009 ID=MsaT046595.1.exon2;Parent=MsaT046595.1
Contig1 CDS 140009 140405 140009 ID=cds.MsaT046595.1;Parent=MsaT046595.1
Contig1 exon 141830 142692 141830 ID=MsaT046595.1.exon3;Parent=MsaT046595.1
Contig1 CDS 141830 142692 141830 ID=cds.MsaT046595.1;Parent=MsaT046595.1
Contig1 exon 142792 142808 142792 ID=MsaT046595.1.exon4;Parent=MsaT046595.1
Contig1 CDS 142792 142808 142792 ID=cds.MsaT046595.1;Parent=MsaT046595.1
Gene Sequence

>MsaT046595.1

ATGTCAAACATCACAAGTTGTGATAGTGGGAGTTTCTCTACAGAGAATACAAGAGAAGATGCAGTTAAACAGCAACAAACTGATCAAATACTTGGTCAATTTCATAGTCCACATTCTCATACTTCAACAAATAATAATAATAATAGCAATGGTTCTAACACAGATTTACAACCACCTCTTAAGAAGAAAAGGAACCTACCAGGAAATCCAGATCCAAGTGCTGAAGTGATAGCACTATCACCAACAACACTAATGGCTACAAACAGATTCGTATGTGAAATATGCAACAAGGGTTTTCAAAGAGACCAAAATCTTCAACTGCACCGAAGAGGACACAACTTGCCTTGGAAGCTAAAACAAAGAACTAGTGCAGAAATTAGAAAGAGGGTTTATGTTTGTCCTGAACCTTCATGTGTTCACCATAACCCTGCAAGAGCACTTGGTGATCTTACTGGTATCAAAAAGCACTTTTGTAGAAAGCATGGTGAGAAAAAGTGGAAATGTGATAAGTGTTCTAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCATTCCAAAATATGTGGTACAAGGGAATACAAATGTGACTGTGGAACCATCTTTTCCAGAAGAGACAGCTTCATTACCCACAGAGCATTCTGTGACGCATTAGCTGAAGAGAACAACAAAGCAAACGAAGGAGTACTATCAAATTTGCAACACCAACCAATCTCAAATCTCGTTTCCTCATTACCTTTAAACCCCATTATTAACCCTCAAATTGGTGGAACTGTATCAGAATTCAACAACCATTCAGATCACAAACTTCCATTATCATCACCTCATGAACTCATGTCAGTAGTGCCACCAAAACCCTTCAACAACAACATATTCACTAGAAGCCTTTCATCATCAACTTCATCTCCTTCTCTTCAACTCAGTTCAAATAATTCACTCAACAACATCTTGGAAGAAAATGGATCACTCCATTTATCTGCTGCAACTTCACCTCACATGTCAGCCACTGCATTGCTACAGAAAGCTGCTCAAATGGGTGCAACTGTGAGTAATAATAATGCAGGTATCATGACGGATAAAACTACCGTTGCTACAAACATGATGGCGCCGCCGCAGTTTGGTGTAGTGCAACAACAAGGACATGCTTTCATGAACCACTACATGCAACAGCAACAACAACACTACAATAATAATTTCAATGCAAATGGGATAATAAATGGAGGAGGCGTTGGAATGAATGGAGTTGATATGTTTAATGCTATATTGGATCAAAGTAAGGCTTTATCGAAGATCATTGAACAAAACAATCAAACTCAAAGCATTAATAGTGTTGCTGGAGGTGGAGGAGGAGGTGCATCAAGTAATAGTAATAATGTTATGAACATTAGTGGAAGTAAGGGTGGTGGAGATGTAATGACACTGGATTTGTTGGGGATAGGAGGAGGAGGTGCACATAGTAATTTCTATGGAGGGGCAACAAGCAACATTTGA
Protein sequence

>MsaT046595.1

MSNITSCDSGSFSTENTREDAVKQQQTDQILGQFHSPHSHTSTNNNNNSNGSNTDLQPPLKKKRNLPGNPDPSAEVIALSPTTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRTSAEIRKRVYVCPEPSCVHHNPARALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKICGTREYKCDCGTIFSRRDSFITHRAFCDALAEENNKANEGVLSNLQHQPISNLVSSLPLNPIINPQIGGTVSEFNNHSDHKLPLSSPHELMSVVPPKPFNNNIFTRSLSSSTSSPSLQLSSNNSLNNILEENGSLHLSAATSPHMSATALLQKAAQMGATVSNNNAGIMTDKTTVATNMMAPPQFGVVQQQGHAFMNHYMQQQQQHYNNNFNANGIINGGGVGMNGVDMFNAILDQSKALSKIIEQNNQTQSINSVAGGGGGGASSNSNNVMNISGSKGGGDVMTLDLLGIGGGGAHSNFYGGATSNI*