Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG046666 | XP_013469068.1 | 98.394 | 996 | 16 | 0 | 1 | 996 | 1 | 996 | 0.0 | 2029 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG046666 | sp|Q10MI0|SRL2_ORYSJ | 49.551 | 1003 | 479 | 16 | 1 | 995 | 1 | 984 | 0.0 | 915 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG046666 | tr|A0A072VN87|A0A072VN87_MEDTR | 98.394 | 996 | 16 | 0 | 1 | 996 | 1 | 996 | 0.0 | 2029 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG046666 | TF | HB-other |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000862 | MsaG046666 | 0.814343 | 2.793233e-51 | 5.311042e-49 |
| MsaG046666 | MsaG046685 | 0.931609 | 8.088450e-94 | 3.758057e-89 |
| MsaG046666 | MsaG032930 | 0.807877 | 6.935257e-50 | 1.125334e-47 |
| MsaG026671 | MsaG046666 | 0.806399 | 1.420820e-49 | 2.225920e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG046666 | MtrunA17_Chr1g0192801 | 98.394 | 996 | 16 | 0 | 1 | 996 | 1 | 996 | 0.0 | 2029 |
| MsaG046666 | MtrunA17_Chr2g0329341 | 34.399 | 1032 | 557 | 25 | 1 | 996 | 1 | 948 | 6.15e-177 | 549 |
| MsaG046666 | MtrunA17_Chr4g0033461 | 33.751 | 1037 | 580 | 23 | 1 | 996 | 1 | 971 | 4.85e-175 | 545 |
| MsaG046666 | MtrunA17_Chr4g0019461 | 33.241 | 1080 | 584 | 27 | 1 | 996 | 1 | 1027 | 6.15e-172 | 538 |
| MsaG046666 | MtrunA17_Chr7g0257661 | 33.815 | 1038 | 590 | 25 | 2 | 996 | 6 | 989 | 6.56e-172 | 537 |
| MsaG046666 | MtrunA17_Chr1g0192791 | 98.246 | 228 | 4 | 0 | 1053 | 1280 | 41 | 268 | 2.64e-160 | 480 |
| MsaG046666 | MtrunA17_Chr5g0413201 | 53.744 | 227 | 89 | 4 | 1053 | 1273 | 94 | 310 | 2.44e-64 | 221 |
| MsaG046666 | MtrunA17_Chr1g0151961 | 49.784 | 231 | 101 | 5 | 1053 | 1277 | 141 | 362 | 1.24e-55 | 198 |
| MsaG046666 | MtrunA17_Chr5g0439391 | 49.123 | 228 | 91 | 4 | 1053 | 1272 | 68 | 278 | 3.17e-53 | 188 |
| MsaG046666 | MtrunA17_Chr2g0289201 | 51.131 | 221 | 94 | 4 | 1053 | 1269 | 139 | 349 | 2.27e-52 | 189 |
| MsaG046666 | MtrunA17_Chr1g0149651 | 38.265 | 196 | 94 | 6 | 1082 | 1257 | 232 | 420 | 1.77e-28 | 120 |
| MsaG046666 | MtrunA17_Chr3g0137241 | 38.614 | 202 | 86 | 6 | 1082 | 1257 | 166 | 355 | 2.10e-28 | 119 |
| MsaG046666 | MtrunA17_Chr2g0305101 | 37.113 | 194 | 98 | 5 | 1082 | 1257 | 152 | 339 | 1.70e-27 | 115 |
| MsaG046666 | MtrunA17_Chr4g0065241 | 38.021 | 192 | 101 | 5 | 1082 | 1257 | 126 | 315 | 1.91e-26 | 112 |
| MsaG046666 | MtrunA17_Chr5g0398181 | 34.555 | 191 | 110 | 3 | 1082 | 1257 | 86 | 276 | 1.08e-24 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG046666 | AT5G26850.4 | 53.181 | 1006 | 435 | 13 | 1 | 996 | 1 | 980 | 0.0 | 1054 |
| MsaG046666 | AT5G26850.3 | 53.181 | 1006 | 435 | 13 | 1 | 996 | 1 | 980 | 0.0 | 1054 |
| MsaG046666 | AT5G26850.1 | 53.181 | 1006 | 435 | 13 | 1 | 996 | 1 | 980 | 0.0 | 1054 |
| MsaG046666 | AT5G26850.2 | 53.181 | 1006 | 435 | 13 | 1 | 996 | 1 | 980 | 0.0 | 1054 |
| MsaG046666 | AT2G41830.1 | 33.238 | 1050 | 615 | 29 | 2 | 996 | 6 | 1024 | 5.21e-162 | 512 |
| MsaG046666 | AT5G21080.1 | 33.586 | 1057 | 600 | 29 | 1 | 994 | 1 | 1018 | 3.24e-161 | 510 |
| MsaG046666 | AT1G05960.1 | 32.817 | 1033 | 605 | 22 | 1 | 996 | 1 | 981 | 3.68e-158 | 501 |
| MsaG046666 | AT1G05960.3 | 32.817 | 1033 | 605 | 22 | 1 | 996 | 1 | 981 | 3.68e-158 | 501 |
| MsaG046666 | AT1G05960.4 | 32.817 | 1033 | 605 | 22 | 1 | 996 | 1 | 981 | 3.68e-158 | 501 |
| MsaG046666 | AT1G05960.2 | 32.258 | 1054 | 604 | 23 | 1 | 996 | 1 | 1002 | 4.93e-154 | 490 |
| MsaG046666 | AT2G41830.2 | 30.326 | 920 | 555 | 29 | 132 | 996 | 1 | 889 | 3.33e-108 | 363 |
| MsaG046666 | AT1G23380.2 | 50.216 | 231 | 95 | 5 | 1051 | 1274 | 107 | 324 | 2.73e-64 | 222 |
| MsaG046666 | AT1G23380.1 | 50.655 | 229 | 93 | 5 | 1053 | 1274 | 107 | 322 | 3.34e-64 | 221 |
| MsaG046666 | AT1G70510.1 | 49.561 | 228 | 97 | 4 | 1053 | 1274 | 91 | 306 | 1.61e-61 | 213 |
| MsaG046666 | AT1G70510.2 | 48.707 | 232 | 97 | 5 | 1053 | 1274 | 91 | 310 | 2.07e-59 | 207 |
| MsaG046666 | AT1G62360.1 | 50.222 | 225 | 96 | 4 | 1053 | 1271 | 143 | 357 | 4.73e-54 | 194 |
| MsaG046666 | AT4G08150.1 | 47.788 | 226 | 104 | 5 | 1053 | 1271 | 156 | 374 | 4.72e-52 | 189 |
| MsaG046666 | AT5G25220.2 | 38.342 | 193 | 98 | 4 | 1080 | 1257 | 217 | 403 | 3.09e-29 | 122 |
| MsaG046666 | AT5G11060.1 | 38.889 | 198 | 88 | 6 | 1081 | 1257 | 182 | 367 | 3.76e-29 | 122 |
| MsaG046666 | AT5G25220.1 | 38.342 | 193 | 98 | 4 | 1080 | 1257 | 217 | 403 | 4.04e-29 | 122 |
| MsaG046666 | AT4G32040.2 | 37.113 | 194 | 104 | 4 | 1080 | 1257 | 171 | 362 | 1.84e-28 | 119 |
| MsaG046666 | AT4G32040.1 | 37.113 | 194 | 104 | 4 | 1080 | 1257 | 171 | 362 | 2.17e-28 | 119 |
| MsaG046666 | AT1G62990.1 | 36.364 | 198 | 96 | 5 | 1082 | 1257 | 86 | 275 | 8.23e-27 | 112 |
Find 0 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Contig1 | gene | 811279 | 837688 | 811279 | ID=MsaG046666 |
| Contig1 | mRNA | 811279 | 837688 | 811279 | ID=MsaT046666.1;Parent=MsaG046666 |
| Contig1 | exon | 811279 | 811333 | 811279 | ID=MsaT046666.1.exon27;Parent=MsaT046666.1 |
| Contig1 | CDS | 811279 | 811333 | 811279 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 811436 | 811593 | 811436 | ID=MsaT046666.1.exon26;Parent=MsaT046666.1 |
| Contig1 | CDS | 811436 | 811593 | 811436 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 812130 | 812392 | 812130 | ID=MsaT046666.1.exon25;Parent=MsaT046666.1 |
| Contig1 | CDS | 812130 | 812392 | 812130 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 816655 | 816802 | 816655 | ID=MsaT046666.1.exon24;Parent=MsaT046666.1 |
| Contig1 | CDS | 816655 | 816802 | 816655 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 816966 | 817076 | 816966 | ID=MsaT046666.1.exon23;Parent=MsaT046666.1 |
| Contig1 | CDS | 816966 | 817076 | 816966 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 818031 | 818060 | 818031 | ID=MsaT046666.1.exon22;Parent=MsaT046666.1 |
| Contig1 | CDS | 818031 | 818060 | 818031 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 819263 | 819335 | 819263 | ID=MsaT046666.1.exon21;Parent=MsaT046666.1 |
| Contig1 | CDS | 819263 | 819335 | 819263 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 825050 | 825116 | 825050 | ID=MsaT046666.1.exon20;Parent=MsaT046666.1 |
| Contig1 | CDS | 825050 | 825116 | 825050 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 830606 | 830708 | 830606 | ID=MsaT046666.1.exon19;Parent=MsaT046666.1 |
| Contig1 | CDS | 830606 | 830708 | 830606 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 831500 | 831706 | 831500 | ID=MsaT046666.1.exon18;Parent=MsaT046666.1 |
| Contig1 | CDS | 831500 | 831706 | 831500 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 831989 | 832126 | 831989 | ID=MsaT046666.1.exon17;Parent=MsaT046666.1 |
| Contig1 | CDS | 831989 | 832126 | 831989 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 832216 | 832350 | 832216 | ID=MsaT046666.1.exon16;Parent=MsaT046666.1 |
| Contig1 | CDS | 832216 | 832350 | 832216 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 832446 | 832637 | 832446 | ID=MsaT046666.1.exon15;Parent=MsaT046666.1 |
| Contig1 | CDS | 832446 | 832637 | 832446 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 832726 | 832850 | 832726 | ID=MsaT046666.1.exon14;Parent=MsaT046666.1 |
| Contig1 | CDS | 832726 | 832850 | 832726 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 832967 | 833051 | 832967 | ID=MsaT046666.1.exon13;Parent=MsaT046666.1 |
| Contig1 | CDS | 832967 | 833051 | 832967 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 833557 | 833706 | 833557 | ID=MsaT046666.1.exon12;Parent=MsaT046666.1 |
| Contig1 | CDS | 833557 | 833706 | 833557 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 833800 | 834204 | 833800 | ID=MsaT046666.1.exon11;Parent=MsaT046666.1 |
| Contig1 | CDS | 833800 | 834204 | 833800 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 834457 | 834603 | 834457 | ID=MsaT046666.1.exon10;Parent=MsaT046666.1 |
| Contig1 | CDS | 834457 | 834603 | 834457 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 834693 | 834976 | 834693 | ID=MsaT046666.1.exon9;Parent=MsaT046666.1 |
| Contig1 | CDS | 834693 | 834976 | 834693 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 835191 | 835387 | 835191 | ID=MsaT046666.1.exon8;Parent=MsaT046666.1 |
| Contig1 | CDS | 835191 | 835387 | 835191 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 835511 | 835713 | 835511 | ID=MsaT046666.1.exon7;Parent=MsaT046666.1 |
| Contig1 | CDS | 835511 | 835713 | 835511 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 835907 | 835954 | 835907 | ID=MsaT046666.1.exon6;Parent=MsaT046666.1 |
| Contig1 | CDS | 835907 | 835954 | 835907 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 836085 | 836213 | 836085 | ID=MsaT046666.1.exon5;Parent=MsaT046666.1 |
| Contig1 | CDS | 836085 | 836213 | 836085 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 836386 | 836500 | 836386 | ID=MsaT046666.1.exon4;Parent=MsaT046666.1 |
| Contig1 | CDS | 836386 | 836500 | 836386 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 836663 | 836778 | 836663 | ID=MsaT046666.1.exon3;Parent=MsaT046666.1 |
| Contig1 | CDS | 836663 | 836778 | 836663 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 836910 | 836984 | 836910 | ID=MsaT046666.1.exon2;Parent=MsaT046666.1 |
| Contig1 | CDS | 836910 | 836984 | 836910 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Contig1 | exon | 837554 | 837688 | 837554 | ID=MsaT046666.1.exon1;Parent=MsaT046666.1 |
| Contig1 | CDS | 837554 | 837688 | 837554 | ID=cds.MsaT046666.1;Parent=MsaT046666.1 |
| Gene Sequence |
| Protein sequence |
