AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046725

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046725 MtrunA17_Chr1g0193311 94.872 156 8 0 1 156 1 156 2.88e-99 282
MsaG046725 MtrunA17_Chr5g0420351 85.897 156 22 0 1 156 1 156 7.12e-95 271
MsaG046725 MtrunA17_Chr7g0218151 78.205 156 34 0 1 156 1 156 1.44e-79 232
MsaG046725 MtrunA17_Chr4g0012761 76.923 156 36 0 1 156 1 156 3.99e-78 229
MsaG046725 MtrunA17_Chr1g0188661 75.641 156 38 0 1 156 1 156 1.59e-77 227
MsaG046725 MtrunA17_Chr1g0188681 69.231 156 48 0 1 156 1 156 2.40e-75 221
MsaG046725 MtrunA17_Chr1g0188651 73.718 156 39 1 1 156 1 154 8.80e-69 205
MsaG046725 MtrunA17_Chr1g0197461 63.399 153 56 0 3 155 2 154 3.32e-65 196
MsaG046725 MtrunA17_Chr1g0197261 59.355 155 63 0 1 155 1 155 1.55e-63 192
MsaG046725 MtrunA17_Chr1g0197321 58.333 156 64 1 1 155 1 156 4.96e-62 188
MsaG046725 MtrunA17_Chr3g0107381 46.104 154 82 1 1 153 1 154 1.78e-42 140
MsaG046725 MtrunA17_Chr3g0110131 50.641 156 76 1 1 155 1 156 5.30e-42 140
MsaG046725 MtrunA17_Chr4g0014321 39.355 155 92 2 1 153 1 155 1.42e-40 135
MsaG046725 MtrunA17_Chr4g0013041 39.610 154 89 3 1 152 1 152 4.45e-36 124
MsaG046725 MtrunA17_Chr8g0351751 40.881 159 90 2 1 155 1 159 4.39e-35 122
MsaG046725 MtrunA17_Chr5g0432691 37.821 156 96 1 1 155 1 156 2.16e-34 118
MsaG046725 MtrunA17_Chr3g0107351 42.208 154 88 1 3 155 2 155 6.21e-34 117
MsaG046725 MtrunA17_Chr4g0014031 42.308 156 88 2 1 154 1 156 8.95e-34 118
MsaG046725 MtrunA17_Chr3g0107331 41.558 154 89 1 3 155 2 155 1.50e-33 115
MsaG046725 MtrunA17_Chr3g0107361 40.260 154 91 1 3 155 2 155 4.23e-32 112
MsaG046725 MtrunA17_Chr3g0107341 39.610 154 92 1 3 155 2 155 1.08e-30 108
MsaG046725 MtrunA17_Chr4g0014131 43.590 156 86 2 1 154 1 156 2.97e-30 109
MsaG046725 MtrunA17_Chr4g0014121 42.949 156 87 2 1 154 29 184 7.75e-30 107
MsaG046725 MtrunA17_Chr3g0107461 35.443 158 98 3 1 155 1 157 1.08e-28 103
MsaG046725 MtrunA17_Chr2g0296831 44.628 121 66 1 1 120 1 121 1.13e-28 102
MsaG046725 MtrunA17_Chr2g0283941 37.748 151 92 2 1 149 1 151 2.72e-28 104
MsaG046725 MtrunA17_Chr4g0031421 36.508 126 78 2 29 152 1 126 2.14e-22 87.0
MsaG046725 MtrunA17_Chr2g0296861 34.194 155 101 1 1 154 1 155 3.39e-22 86.7
MsaG046725 MtrunA17_Chr3g0105791 35.714 126 79 2 29 152 1 126 8.03e-22 85.5
MsaG046725 MtrunA17_Chr3g0106421 33.333 129 84 2 29 155 1 129 1.77e-19 81.3
MsaG046725 MtrunA17_Chr4g0008701 37.903 124 69 3 1 120 1 120 1.99e-19 84.0
MsaG046725 MtrunA17_Chr1g0162261 46.237 93 49 1 64 155 22 114 5.67e-19 77.4
MsaG046725 MtrunA17_Chr3g0106501 34.109 129 83 2 29 155 1 129 1.17e-18 77.4
MsaG046725 MtrunA17_Chr7g0267601 37.168 113 67 2 1 109 1 113 2.98e-18 80.5
MsaG046725 MtrunA17_Chr1g0161931 45.000 100 54 1 57 155 21 120 2.66e-17 73.2
MsaG046725 MtrunA17_Chr1g0162181 45.556 90 48 1 64 152 22 111 3.82e-16 70.1
MsaG046725 MtrunA17_Chr6g0449861 31.818 110 69 3 1 104 9 118 1.82e-13 66.6
MsaG046725 MtrunA17_Chr3g0128721 28.571 133 89 2 3 134 6 133 2.42e-13 66.2
MsaG046725 MtrunA17_Chr6g0457851 29.091 110 72 3 1 104 11 120 2.64e-12 63.2
MsaG046725 MtrunA17_Chr1g0212151 28.182 110 73 2 1 104 1 110 1.82e-11 60.8
MsaG046725 MtrunA17_Chr5g0427401 29.221 154 85 5 1 148 1 136 9.18e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046725 AT5G26630.1 44.872 156 85 1 1 155 1 156 9.14e-38 129
MsaG046725 AT5G48670.1 46.795 156 82 1 1 155 1 156 2.48e-35 125
MsaG046725 AT3G05860.2 40.268 149 88 1 1 148 1 149 4.53e-33 116
MsaG046725 AT1G65330.1 40.909 154 90 1 3 155 2 155 5.72e-33 118
MsaG046725 AT3G05860.3 40.268 149 88 1 1 148 1 149 1.84e-32 115
MsaG046725 AT3G05860.1 40.268 149 88 1 1 148 1 149 2.81e-32 115
MsaG046725 AT1G65300.1 38.312 154 94 1 3 155 2 155 3.40e-31 113
MsaG046725 AT5G26650.1 35.948 153 97 1 4 155 2 154 2.80e-27 104
MsaG046725 AT5G27960.1 34.641 153 99 1 4 155 2 154 3.58e-25 98.6
MsaG046725 AT1G31630.1 30.464 151 103 2 3 152 2 151 1.87e-24 97.1
MsaG046725 AT1G22590.2 32.051 156 102 2 1 155 1 153 3.27e-24 92.4
MsaG046725 AT1G31640.1 34.459 148 95 2 3 149 2 148 7.70e-23 93.6
MsaG046725 AT5G27810.1 44.444 90 49 1 29 117 1 90 7.92e-21 82.4
MsaG046725 AT2G28700.1 34.746 118 76 1 1 117 1 118 4.31e-20 85.1
MsaG046725 AT5G26580.1 32.903 155 91 2 1 155 1 142 1.79e-19 83.6
MsaG046725 AT5G06500.1 36.301 146 80 5 1 141 1 138 8.38e-18 77.8
MsaG046725 AT2G40210.1 34.884 129 81 2 1 127 1 128 5.09e-14 68.6
MsaG046725 AT5G55690.1 26.582 158 95 3 1 151 1 144 3.69e-12 62.8
MsaG046725 AT5G55690.2 26.582 158 95 3 1 151 1 144 3.69e-12 62.8
MsaG046725 AT1G47760.1 33.333 111 67 3 1 110 1 105 7.50e-12 60.8
MsaG046725 AT5G58890.1 34.234 111 65 3 1 104 1 110 1.00e-11 61.6

Find 0 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Contig1 gene 1537938 1538408 1537938 ID=MsaG046725
Contig1 mRNA 1537938 1538408 1537938 ID=MsaT046725.1;Parent=MsaG046725
Contig1 exon 1537938 1538408 1537938 ID=MsaT046725.1.exon1;Parent=MsaT046725.1
Contig1 CDS 1537938 1538408 1537938 ID=cds.MsaT046725.1;Parent=MsaT046725.1
Gene Sequence

>MsaT046725.1

ATGACTAGGAAGAAGGTGAAACTTGCTTTTATCTCTGATGATTCGGCAAGAAAGGCAACCTATAAGAAAAGAAAAAAGGGTATCATCAAAAAGGTGAGCGAACTCACCATTCTATGCGGTATTCCAGCTTGTGCTATAATTTCCAATCCTTTTGATTCTAAAACAGAGGTGTGGCCAGATCTAGAGAAGGCTAAACAGGTGATTGAGAGGTATCAAAATTCATCGGTGAAAGATGAAACAAAGAATGTGAACCAAGAGAGCTTCCTATTGCAGAGGATTACCAAAGCTAGGGAACATCTTCAAAAGCAAAGGCACGACAATCGTGAGAAGGAGTTGAACGTTCTTATGATTGAGTACATGAAAAACAAAAAGCTGCCGGATGGTTTGACCGTTTCGGACTTGAAAGAGTTTGATAAGCTAATTGAGAAAAATATGAAGGAGATTGATAACAAGATTGATGCACTCGGTTGA
Protein sequence

>MsaT046725.1

MTRKKVKLAFISDDSARKATYKKRKKGIIKKVSELTILCGIPACAIISNPFDSKTEVWPDLEKAKQVIERYQNSSVKDETKNVNQESFLLQRITKAREHLQKQRHDNREKELNVLMIEYMKNKKLPDGLTVSDLKEFDKLIEKNMKEIDNKIDALG*