AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG046790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046790 MtrunA17_Chr1g0194121 97.308 1003 26 1 1 1002 1 1003 0.0 2033
MsaG046790 MtrunA17_Chr7g0270541 75.149 1010 226 11 8 1002 2 1001 0.0 1493
MsaG046790 MtrunA17_Chr2g0302791 48.555 1038 471 17 7 1001 7 1024 0.0 910
MsaG046790 MtrunA17_Chr1g0162871 33.558 593 361 11 417 1001 435 1002 1.76e-87 302
MsaG046790 MtrunA17_Chr1g0162871 49.697 165 74 4 115 273 74 235 1.33e-37 152
MsaG046790 MtrunA17_Chr8g0360361 67.033 91 30 0 146 236 64 154 2.44e-33 127
MsaG046790 MtrunA17_Chr8g0334671 56.522 115 41 2 116 221 147 261 2.27e-32 129
MsaG046790 MtrunA17_Chr8g0386271 58.511 94 39 0 133 226 85 178 3.10e-31 127
MsaG046790 MtrunA17_Chr4g0046991 62.921 89 33 0 133 221 59 147 1.16e-30 118
MsaG046790 MtrunA17_Chr5g0420051 61.798 89 34 0 132 220 166 254 1.43e-30 126
MsaG046790 MtrunA17_Chr7g0231911 58.511 94 39 0 137 230 139 232 2.35e-30 122
MsaG046790 MtrunA17_Chr2g0308321 64.773 88 31 0 133 220 157 244 3.16e-30 126
MsaG046790 MtrunA17_Chr2g0282521 64.130 92 32 1 133 224 51 141 3.37e-30 116
MsaG046790 MtrunA17_Chr4g0061651 69.737 76 23 0 145 220 121 196 4.16e-29 122
MsaG046790 MtrunA17_Chr2g0286661 25.507 345 224 9 430 751 260 594 1.27e-28 123
MsaG046790 MtrunA17_Chr2g0286661 48.315 89 46 0 132 220 88 176 1.27e-20 97.8
MsaG046790 MtrunA17_Chr3g0122411 56.842 95 41 0 132 226 150 244 1.55e-28 120
MsaG046790 MtrunA17_Chr7g0224821 44.853 136 53 2 106 220 16 150 2.66e-28 117
MsaG046790 MtrunA17_Chr3g0132091 50.435 115 54 2 133 244 58 172 3.50e-28 117
MsaG046790 MtrunA17_Chr7g0258471 68.919 74 23 0 147 220 142 215 2.14e-27 116
MsaG046790 MtrunA17_Chr8g0377421 56.250 80 35 0 147 226 132 211 9.80e-24 103
MsaG046790 MtrunA17_Chr8g0377401 58.108 74 31 0 146 219 161 234 2.51e-21 94.7
MsaG046790 MtrunA17_Chr2g0317041 61.290 62 24 0 132 193 84 145 1.52e-19 86.7
MsaG046790 MtrunA17_Chr1g0175031 61.702 47 18 0 147 193 74 120 7.77e-13 66.6
MsaG046790 MtrunA17_Chr8g0377431 45.070 71 39 0 132 202 136 206 3.01e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG046790 AT3G60030.1 56.144 1001 341 26 16 1002 11 927 0.0 990
MsaG046790 AT2G47070.1 56.083 1011 293 31 6 1002 8 881 0.0 990
MsaG046790 AT2G47070.2 58.502 841 251 22 171 1002 1 752 0.0 885
MsaG046790 AT1G20980.1 32.308 585 355 14 427 1001 481 1034 3.12e-79 280
MsaG046790 AT1G20980.1 41.393 244 94 7 26 260 38 241 6.99e-39 157
MsaG046790 AT1G76580.1 30.743 579 356 13 429 1001 480 1019 6.11e-76 270
MsaG046790 AT1G76580.1 39.912 228 86 5 30 252 40 221 1.13e-35 147
MsaG046790 AT1G76580.2 39.912 228 86 5 30 252 40 221 2.35e-36 147
MsaG046790 AT1G76580.2 35.909 220 133 4 429 646 480 693 1.03e-31 133
MsaG046790 AT1G76580.3 39.912 228 86 5 30 252 40 221 2.79e-36 147
MsaG046790 AT1G76580.3 36.239 218 131 4 429 644 480 691 4.92e-31 131
MsaG046790 AT1G69170.1 65.909 88 30 0 133 220 110 197 5.96e-32 130
MsaG046790 AT2G33810.1 60.674 89 35 0 134 222 41 129 1.34e-31 120
MsaG046790 AT1G02065.1 55.833 120 43 4 116 225 147 266 1.47e-31 127
MsaG046790 AT1G53160.2 66.667 81 27 0 146 226 53 133 1.45e-30 119
MsaG046790 AT1G53160.1 66.667 81 27 0 146 226 53 133 1.45e-30 119
MsaG046790 AT1G53160.3 66.667 81 27 0 146 226 53 133 1.68e-30 119
MsaG046790 AT3G15270.1 58.163 98 38 1 136 230 49 146 8.56e-30 117
MsaG046790 AT5G43270.3 56.701 97 42 0 147 243 169 265 6.09e-29 121
MsaG046790 AT5G43270.1 56.701 97 42 0 147 243 169 265 6.09e-29 121
MsaG046790 AT5G43270.2 56.701 97 42 0 147 243 169 265 6.09e-29 121
MsaG046790 AT5G50570.1 54.255 94 41 1 140 233 94 185 2.71e-28 118
MsaG046790 AT5G50570.2 54.255 94 41 1 140 233 94 185 2.71e-28 118
MsaG046790 AT5G50670.1 54.255 94 41 1 140 233 94 185 2.71e-28 118
MsaG046790 AT5G50670.2 54.255 94 41 1 140 233 94 185 2.71e-28 118
MsaG046790 AT1G27370.6 50.450 111 51 2 110 220 143 249 8.66e-28 116
MsaG046790 AT1G27370.1 50.450 111 51 2 110 220 143 249 1.11e-27 117
MsaG046790 AT1G27370.5 50.450 111 51 2 110 220 143 249 1.11e-27 117
MsaG046790 AT1G27370.3 50.450 111 51 2 110 220 143 249 1.11e-27 117
MsaG046790 AT1G27370.2 50.450 111 51 2 110 220 143 249 1.11e-27 117
MsaG046790 AT1G27370.4 50.450 111 51 2 110 220 143 249 1.11e-27 117
MsaG046790 AT1G27370.7 50.450 111 51 2 110 220 143 249 1.11e-27 117
MsaG046790 AT1G27360.2 50.450 111 50 2 110 220 143 248 4.31e-26 112
MsaG046790 AT1G27360.1 50.450 111 50 2 110 220 143 248 4.31e-26 112
MsaG046790 AT1G27360.3 50.450 111 50 2 110 220 143 248 4.31e-26 112
MsaG046790 AT1G27360.4 50.450 111 50 2 110 220 143 248 4.31e-26 112
MsaG046790 AT3G57920.1 58.333 96 34 1 131 220 37 132 4.94e-26 111
MsaG046790 AT5G18830.2 26.984 315 195 7 382 672 239 542 8.07e-26 115
MsaG046790 AT5G18830.2 54.545 88 39 1 133 220 125 211 2.15e-22 103
MsaG046790 AT5G18830.1 26.984 315 195 7 382 672 239 542 1.02e-25 114
MsaG046790 AT5G18830.1 54.545 88 39 1 133 220 125 211 2.42e-22 103
MsaG046790 AT5G18830.3 26.984 315 195 7 382 672 239 542 1.05e-25 114
MsaG046790 AT5G18830.3 54.545 88 39 1 133 220 125 211 2.28e-22 103
MsaG046790 AT2G42200.1 66.216 74 25 0 147 220 74 147 1.08e-25 110
MsaG046790 AT1G02065.2 50.562 89 34 4 116 194 147 235 2.96e-16 80.1

Find 0 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Contig1 gene 2303664 2310974 2303664 ID=MsaG046790
Contig1 mRNA 2303664 2310974 2303664 ID=MsaT046790.1;Parent=MsaG046790
Contig1 exon 2303664 2304241 2303664 ID=MsaT046790.1.exon1;Parent=MsaT046790.1
Contig1 CDS 2303664 2304241 2303664 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2305560 2305741 2305560 ID=MsaT046790.1.exon2;Parent=MsaT046790.1
Contig1 CDS 2305560 2305741 2305560 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2306984 2307567 2306984 ID=MsaT046790.1.exon3;Parent=MsaT046790.1
Contig1 CDS 2306984 2307567 2306984 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2308154 2308228 2308154 ID=MsaT046790.1.exon4;Parent=MsaT046790.1
Contig1 CDS 2308154 2308228 2308154 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2308319 2308426 2308319 ID=MsaT046790.1.exon5;Parent=MsaT046790.1
Contig1 CDS 2308319 2308426 2308319 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2308530 2308647 2308530 ID=MsaT046790.1.exon6;Parent=MsaT046790.1
Contig1 CDS 2308530 2308647 2308530 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2308742 2308883 2308742 ID=MsaT046790.1.exon7;Parent=MsaT046790.1
Contig1 CDS 2308742 2308883 2308742 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2309041 2309185 2309041 ID=MsaT046790.1.exon8;Parent=MsaT046790.1
Contig1 CDS 2309041 2309185 2309041 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2309488 2310123 2309488 ID=MsaT046790.1.exon9;Parent=MsaT046790.1
Contig1 CDS 2309488 2310123 2309488 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Contig1 exon 2310534 2310974 2310534 ID=MsaT046790.1.exon10;Parent=MsaT046790.1
Contig1 CDS 2310534 2310974 2310534 ID=cds.MsaT046790.1;Parent=MsaT046790.1
Gene Sequence

>MsaT046790.1

ATGGGGGAAAGATTGGGTGCTGAAAATTATCATTTTTATGGTGTTGGTGGTTCATCTGATTTGAGTGGGATGGGGAAGAGGTCAAGGGAATGGAATTTGAATGATTGGAGATGGGATGGTGATTTGTTTATAGCTAGCCGTGTGAATCCGGTGCAGGCGGAGAGTTTGCGAGTGGGACAACAGTTTTTCCCACTCGGGTCTGGGATCCCGGTGGTTGGAGGTTCGTCCAATACGTCTTCTTCATGCTCCGAGGAAGTAGATCTTGAGAAAGGGAATAAGGAAGGGGATAGGAAAAGGAGAGTTATTGTGCTGGAAGATGATGGTTTGAATGATGAAGCTGGTGCACTTAGCTTAAATCTTGCAGGACATGTTTCACCGGCGGTGGAAAGGGACGGAAAAAAGAGCAGAGTAGCTGGAGGAATTTCGAATCGAGCTGTTTGTCAGGTTGAAGACTGTGGTGCAGATCTGAGTAGAGGTAAGGATTATCACAGACGTCATAAAGTTTGTGAGATGCATTCTAAGGCTAGTAGGGCTCTTGTGGGAAATGCAATGCAGAGGTTTTGTCAACAGTGTAGTAGGTTTCACATACTTCAAGAGTTCGATGAAGGAAAGAGAAGCTGTCGAAGACGCTTGGCTGGCCATAACAAACGGAGAAGGAAAACAAATCAAGAAGCTGTTCCTAATGGAAGTCCAACAAATGATGATCAGACTAGTAGTTATCTGTTGATTAGTTTATTGAAGATACTTTCAAATATGCATTCTGACAGGTCAGATCAGCCTACAGATCAGGATCTATTAACTCATCTTCTAAGGAGTCTTGCAAGTCAGAATGATGAACAAGGGAGCAAGAACTTGTCAAACCTTTTACGAGAACAAGAGAATTTGTTGAGAGAAGGGGGTTCTTCAAGAAATTCAGGGATGGTATCAGCTTTATTCTCCAATGGTTCTCAGGGGTCTCCAACTGTTATAACACAACATCAAACTGTATCTATGAATCAAATGCAACAAGAAATGGTGCATACACATGATGTTAGAACCTCTGATCATCAACTCATATCTTCAATAAAACCCAGCATTTCAAACAGTCCCCCGGCTTACTCGGAAGCAAGAGATAGCAGTGGACAAACAAAGATGAATAATTTTGATTTGAATGACATATATGTTGACTCAGATGATGGAACGGAAGATCTAGAAAGACTGCCCGTCTCTACAAATCTTGCTACTAGCTCTGTTGACTACCCTTGGACACAACAAGATTCTCATCAGTCAAGTCCACCTCAAACAAGTGGAAATTCAGATTCTGCATCTGCCCAGTCCCCTTCTAGTTCTAGCGGAGAAGCCCAGAGTCGCACAGACCGAATTGTTTTTAAACTCTTCGGTAAAGAGCCAAATGAATTTCCTCTTGTACTTAGAGCACAGATTCTAGATTGGTTATCCCACAGTCCTACTGATATTGAGAGCTACATACGCCCTGGTTGTATTGTTTTGACCATTTATCTGCGTCAGGCCGAGGCTGTGTGGGAGGAACTTTGCTGTGATCTGACTTCCAGTTTGATTAAGCTGCTGGATGTCTCAGATGATACGTTTTGGAAAACTGGATGGGTTCATATCAGAGTGCAACATCAAATGGCATTCATTTTCAACGGTCAAGTTGTCATAGATACATCACTACCTTTCCGAAGCAACAATTATAGCAAAATTTGGACTGTTAGTCCAATTGCTGTACCGGCATCAAAGAGAGCTCAATTTTCTGTTAAAGGTGTCAACCTGATGCGCCCTGCCACAAGGTTAATGTGTGCTTTAGAAGGAAAATATCTGGTCTGTGAAGATGCTCATGAGTCAACGGATCAATACTCCGAGGAGCTTGATGAACTTCAATGCATCCAATTTTCATGTTCTGTACCTGTGACAAATGGAAGAGGATTTATTGAGATTGAGGACCAGGGTTTGAGTAGCAGCTTCTTTCCTTTCATAGTGGCAGAAGAGGATGTTTGCTCAGAGATCCGCGTGCTTGAACCTCTAATAGAATCAAGTGAAACTGCTTCAGATATTGAAGGGACTGGAAAAATTAAAGCCAAAAGTCAAGCTATGGATTTCATTCATGAAATGGGCTGGCTTCTTCACAGAAGCCAATTGAAATATAGGATGGTTAACTTGAACTCTGGTGTAGATCTCTTTCCATTAGAACGGTTCACATGGCTTATGGAGTTTTCAATGGACCATGATTGGTGTGCAGTGGTGAAAAAACTGTTGAACCTACTCTTTGATGAAACTGTTAATAAAGGAGATCACCCTACATTGTATCAAGCACTTTCAGAGATGGGTCTTCTTCACAGAGCTGTAAGGAGAAATAGCAAACAGTTAGTGGAGCTGCTTTTGCGATATGTTCCTGACAATACATCTGATGAACTAGGACCCAAAGAAAAGGCGCTTGTTGGTGGAAAGAATCATAGCTACTTGTTTAGACCTGATGCTGTTGGTCCTGCCGGTTTGACACCACTTCATATAGCAGCGGGAAAAGATGGTTCCGAGGATGTACTGGATGCTCTGACTAATGATCCTTGCATGGTGGGAATTGAGGCATGGAAGAATGCCCGTGACAGTACAGGCTCCACACCTGAGGATTATGCGCGATTACGTGGCCATTATACGTACATTCACTTAGTGCAGAAAAAAATTAACAAAACACAAGGAGCAGCTCATGTGGTGGTTGAGATTCCCAGCAATACAGAGAGCAATAAAACCCCGAAGCAAAATGAGTCATTCGCTAGCTTAGAGATTGGAAAAGCTGAAGTAAGACGTAGCCGAGGGAACTGTAAACTTTGTGATACTAAAATTTCTTGTAGAACTGCAGTAGGAAGGTCTATGGTATACAGACCTGCAATGCTCTCTATGGTGGCCATAGCAGCGGTCTGTGTTTGTGTGGCACTTCTGTTTAAGAGCTCACCCGAAGTTCTATACATGTTCCGACCTTTCAGATGGGAATCATTGGAGTTCGGAACAAGCTAA
Protein sequence

>MsaT046790.1

MGERLGAENYHFYGVGGSSDLSGMGKRSREWNLNDWRWDGDLFIASRVNPVQAESLRVGQQFFPLGSGIPVVGGSSNTSSSCSEEVDLEKGNKEGDRKRRVIVLEDDGLNDEAGALSLNLAGHVSPAVERDGKKSRVAGGISNRAVCQVEDCGADLSRGKDYHRRHKVCEMHSKASRALVGNAMQRFCQQCSRFHILQEFDEGKRSCRRRLAGHNKRRRKTNQEAVPNGSPTNDDQTSSYLLISLLKILSNMHSDRSDQPTDQDLLTHLLRSLASQNDEQGSKNLSNLLREQENLLREGGSSRNSGMVSALFSNGSQGSPTVITQHQTVSMNQMQQEMVHTHDVRTSDHQLISSIKPSISNSPPAYSEARDSSGQTKMNNFDLNDIYVDSDDGTEDLERLPVSTNLATSSVDYPWTQQDSHQSSPPQTSGNSDSASAQSPSSSSGEAQSRTDRIVFKLFGKEPNEFPLVLRAQILDWLSHSPTDIESYIRPGCIVLTIYLRQAEAVWEELCCDLTSSLIKLLDVSDDTFWKTGWVHIRVQHQMAFIFNGQVVIDTSLPFRSNNYSKIWTVSPIAVPASKRAQFSVKGVNLMRPATRLMCALEGKYLVCEDAHESTDQYSEELDELQCIQFSCSVPVTNGRGFIEIEDQGLSSSFFPFIVAEEDVCSEIRVLEPLIESSETASDIEGTGKIKAKSQAMDFIHEMGWLLHRSQLKYRMVNLNSGVDLFPLERFTWLMEFSMDHDWCAVVKKLLNLLFDETVNKGDHPTLYQALSEMGLLHRAVRRNSKQLVELLLRYVPDNTSDELGPKEKALVGGKNHSYLFRPDAVGPAGLTPLHIAAGKDGSEDVLDALTNDPCMVGIEAWKNARDSTGSTPEDYARLRGHYTYIHLVQKKINKTQGAAHVVVEIPSNTESNKTPKQNESFASLEIGKAEVRRSRGNCKLCDTKISCRTAVGRSMVYRPAMLSMVAIAAVCVCVALLFKSSPEVLYMFRPFRWESLEFGTS*