AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0002910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0002910 MtrunA17_Chr1g0169431 90.226 266 4 1 1 266 1 244 1.05e-176 486
Msa0002910 MtrunA17_Chr5g0395391 65.759 257 51 3 1 257 12 231 1.33e-101 300
Msa0002910 MtrunA17_Chr5g0395391 76.147 109 26 0 158 266 234 342 5.03e-56 183
Msa0002910 MtrunA17_Chr8g0340401 47.340 188 84 7 85 263 114 295 1.05e-38 138
Msa0002910 MtrunA17_Chr8g0372291 72.000 75 21 0 192 266 216 290 8.57e-36 129
Msa0002910 MtrunA17_Chr3g0140331 38.571 280 118 11 7 263 74 322 1.83e-35 130
Msa0002910 MtrunA17_Chr2g0326771 46.296 162 55 4 120 258 128 280 1.92e-35 129
Msa0002910 MtrunA17_Chr1g0146231 78.571 70 15 0 194 263 234 303 1.36e-34 127
Msa0002910 MtrunA17_Chr3g0145121 75.000 72 18 0 192 263 213 284 9.43e-34 124
Msa0002910 MtrunA17_Chr4g0035891 50.000 140 44 5 146 261 141 278 2.93e-33 122
Msa0002910 MtrunA17_Chr5g0404981 71.233 73 21 0 194 266 300 372 6.86e-33 124
Msa0002910 MtrunA17_Chr7g0253101 74.648 71 18 0 188 258 182 252 1.38e-32 120
Msa0002910 MtrunA17_Chr6g0461591 76.471 68 16 0 193 260 191 258 1.43e-32 120
Msa0002910 MtrunA17_Chr1g0199671 70.130 77 21 1 182 258 232 306 5.67e-32 120
Msa0002910 MtrunA17_Chr4g0028961 74.627 67 17 0 194 260 232 298 6.77e-32 119
Msa0002910 MtrunA17_Chr1g0213161 71.642 67 19 0 192 258 150 216 3.19e-31 115
Msa0002910 MtrunA17_Chr7g0232671 55.224 67 28 1 194 260 187 251 8.53e-19 83.2
Msa0002910 MtrunA17_Chr7g0232701 47.126 87 44 2 171 255 48 134 7.89e-18 77.8
Msa0002910 MtrunA17_Chr7g0232681 46.739 92 45 3 171 260 107 196 2.95e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0002910 AT3G24050.1 80.000 75 15 0 192 266 192 266 6.98e-40 139
Msa0002910 AT4G32890.1 38.298 282 114 9 7 263 20 266 1.30e-38 137
Msa0002910 AT4G34680.1 47.183 142 62 3 133 264 112 250 1.09e-34 126
Msa0002910 AT4G34680.2 47.183 142 62 3 133 264 112 250 1.09e-34 126
Msa0002910 AT5G66320.2 79.412 68 14 0 194 261 249 316 2.68e-34 127
Msa0002910 AT5G66320.1 79.412 68 14 0 194 261 249 316 2.68e-34 127
Msa0002910 AT5G25830.1 77.143 70 16 0 194 263 219 288 2.74e-33 124
Msa0002910 AT3G60530.1 75.362 69 17 0 193 261 157 225 5.97e-33 120
Msa0002910 AT3G51080.1 72.603 73 20 0 194 266 221 293 7.98e-33 122
Msa0002910 AT4G36240.1 35.273 275 104 6 6 261 12 231 9.25e-33 120
Msa0002910 AT1G08010.2 72.464 69 19 0 192 260 218 286 6.81e-32 119
Msa0002910 AT1G08010.1 72.464 69 19 0 192 260 218 286 6.81e-32 119
Msa0002910 AT1G08010.3 72.464 69 19 0 192 260 218 286 6.81e-32 119
Msa0002910 AT2G45050.1 75.000 68 17 0 194 261 179 246 7.99e-32 118
Msa0002910 AT1G08000.2 41.935 186 64 6 114 260 104 284 9.99e-32 119
Msa0002910 AT1G08000.1 41.935 186 64 6 114 260 104 284 9.99e-32 119
Msa0002910 AT3G54810.1 75.000 68 17 0 194 261 229 296 1.33e-30 116
Msa0002910 AT3G54810.2 75.000 68 17 0 194 261 229 296 1.33e-30 116
Msa0002910 AT3G45170.1 43.165 139 63 3 123 261 60 182 3.93e-27 104
Msa0002910 AT2G28340.1 66.667 63 21 0 196 258 193 255 2.58e-25 102

Find 105 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACAAGCAAAATGATTTA+AGG 0.141755 1_1:-5223536 None:intergenic
TCAGTCTCTCAATTCTCTTT+TGG 0.180061 1_1:+5220756 Msa0002910:five_prime_UTR
CATGCCAAAAGTTAGGATTT+TGG 0.206218 1_1:+5223401 Msa0002910:three_prime_UTR
TGCTTATCTGAACTAATTCT+AGG 0.234887 1_1:+5223675 Msa0002910:three_prime_UTR
ATGTACTTTGATTGAGGATT+AGG 0.241750 1_1:+5223612 Msa0002910:three_prime_UTR
AGTGAAGGAAAGGCTTTCTT+AGG 0.280723 1_1:-5222197 None:intergenic
GTCCAACAAAGATGCATTTC+CGG 0.285678 1_1:+5222814 Msa0002910:CDS
GACATTTCCTCCCAATGGTT+GGG 0.293078 1_1:-5223114 None:intergenic
CTATTTGCAGGACGGTATTC+AGG 0.302785 1_1:-5223241 None:intergenic
CGAAAAGTTGGGCTATTTGC+AGG 0.311001 1_1:-5223253 None:intergenic
CATGCATTACAAAGTGTCTT+TGG 0.315428 1_1:-5223187 None:intergenic
CATTTAGCATGCCAAAAGTT+AGG 0.327033 1_1:+5223394 Msa0002910:three_prime_UTR
AAAACCAAAATCCTAACTTT+TGG 0.331501 1_1:-5223405 None:intergenic
CAAAGTGTCTTTGGACCATT+TGG 0.337970 1_1:-5223178 None:intergenic
AGAATCAATGTATTGGCATT+TGG 0.339961 1_1:+5222494 Msa0002910:intron
TGTACTTTGATTGAGGATTA+GGG 0.355942 1_1:+5223613 Msa0002910:three_prime_UTR
GAAGGTGGATCACTGCATTC+TGG 0.356189 1_1:-5222224 None:intergenic
CTCTCTTGCCTGCTCCTAGC+TGG 0.362394 1_1:+5220795 Msa0002910:five_prime_UTR
GTTGTTTCCACGGGAATTGT+AGG 0.366200 1_1:-5223054 None:intergenic
TGGTTCTGTGGACGACCTCT+TGG 0.388206 1_1:+5222133 Msa0002910:CDS
GATGAGGAAGCAGAAGGGAA+TGG 0.399465 1_1:+5223315 Msa0002910:CDS
TTTGTAATGCATGTGGGGTT+AGG 0.415099 1_1:+5223197 Msa0002910:CDS
ACTTACAGAAAGTGAATTGA+TGG 0.418526 1_1:-5222273 None:intergenic
CCACGGGAATTGTAGGTTTG+AGG 0.425374 1_1:-5223047 None:intergenic
AAGCAGAAGGGAATGGGAAC+GGG 0.428545 1_1:+5223322 Msa0002910:CDS
TGTGATTGTAGCCACTTAAG+AGG 0.429240 1_1:-5222961 None:intergenic
TGAATTGTCGTGTATTCAAC+CGG 0.432782 1_1:+5222853 Msa0002910:CDS
AGCCGGAAATGCATCTTTGT+TGG 0.438765 1_1:-5222816 None:intergenic
TGCATGTCACTGCGAAAAGT+TGG 0.440834 1_1:-5223265 None:intergenic
TTGTGCTGTTCTCGAGCAGT+GGG 0.447298 1_1:-5222901 None:intergenic
ACATTTCCTCCCAATGGTTG+GGG 0.449081 1_1:-5223113 None:intergenic
ATTTGATGCTTCAAAAGATC+CGG 0.450692 1_1:-5222872 None:intergenic
GACTTGCTCCGTGCACGGAC+AGG 0.458205 1_1:-5222992 None:intergenic
TCAACAAATGTTTCAACAGC+CGG 0.462728 1_1:-5222833 None:intergenic
GAATCTGTCATTTGCACCGC+AGG 0.464321 1_1:-5222536 None:intergenic
TTCGCTCAATCCATTGGCCC+TGG 0.469600 1_1:+5222244 Msa0002910:CDS
GACAAGAGCCAGCTAGGAGC+AGG 0.470245 1_1:-5220803 Msa0002900:CDS
CTTGTGCTGTTCTCGAGCAG+TGG 0.473765 1_1:-5222902 None:intergenic
CCAGGAGGAGGTTGCAGAAG+AGG 0.477241 1_1:+5222778 Msa0002910:intron
GTGGAGATGAGGAAGCAGAA+GGG 0.478182 1_1:+5223310 Msa0002910:CDS
TTGCAGAAGAGGAGCTTGAA+TGG 0.482772 1_1:+5222789 Msa0002910:CDS
ATGAGGAAGCAGAAGGGAAT+GGG 0.486460 1_1:+5223316 Msa0002910:CDS
GTGAAACAAACCCCAACCAT+TGG 0.493226 1_1:+5223103 Msa0002910:CDS
TTGGTACTCGGTTGTTTCCA+CGG 0.494137 1_1:-5223064 None:intergenic
TTGAGAGACAAGAGCCAGCT+AGG 0.500487 1_1:-5220809 Msa0002900:CDS
AACAGCATTACCCTCTTAAG+TGG 0.500575 1_1:+5222950 Msa0002910:CDS
GCATGTCACTGCGAAAAGTT+GGG 0.502991 1_1:-5223264 None:intergenic
TGGTACTCGGTTGTTTCCAC+GGG 0.505676 1_1:-5223063 None:intergenic
CAATGGCGTGCAGGTCCAAA+TGG 0.508669 1_1:+5223163 Msa0002910:CDS
TTGGATTTCTCATCTGACAT+AGG 0.508816 1_1:+5222152 Msa0002910:CDS
AGTGGAGATGAGGAAGCAGA+AGG 0.514467 1_1:+5223309 Msa0002910:CDS
TTCTGGTTTGAGTGAAGGAA+AGG 0.515866 1_1:-5222207 None:intergenic
TGACATTTCCTCCCAATGGT+TGG 0.516568 1_1:-5223115 None:intergenic
TGTGGGGTTAGGTATAAGTC+TGG 0.520333 1_1:+5223208 Msa0002910:CDS
GGTAACATGTACTTTGATTG+AGG 0.520464 1_1:+5223606 Msa0002910:three_prime_UTR
TCCACCTTCGCTCAATCCAT+TGG 0.521499 1_1:+5222238 Msa0002910:CDS
GATGGAGGCCCTTGGTTCTG+TGG 0.522422 1_1:+5222121 Msa0002910:exon
TGAAACAAACCCCAACCATT+GGG 0.524385 1_1:+5223104 Msa0002910:CDS
GTTCAGATAAGCATAATGAA+TGG 0.527844 1_1:-5223665 None:intergenic
AATTCTAGGTTGATGAATAG+TGG 0.527863 1_1:+5223689 Msa0002910:three_prime_UTR
GTGAATTGATGGGATCATCC+AGG 0.528433 1_1:-5222262 None:intergenic
ATGGGATCATCCAGGGCCAA+TGG 0.530010 1_1:-5222254 None:intergenic
CTGCATTCTGGTTTGAGTGA+AGG 0.532294 1_1:-5222212 None:intergenic
TCATCTCCACTACTTTCCTG+TGG 0.533667 1_1:-5223297 None:intergenic
TCTCGTAGCAAGCCTCGCCT+TGG 0.534627 1_1:+5223013 Msa0002910:CDS
AGTACACAAACGTGAAAAGC+TGG 0.538176 1_1:+5220834 Msa0002910:five_prime_UTR
GATACTCCACAATGGCGTGC+AGG 0.539263 1_1:+5223154 Msa0002910:CDS
TTGAGGCATCAGCAAGGCCA+AGG 0.541565 1_1:-5223030 None:intergenic
GAAGCAGAAGGGAATGGGAA+CGG 0.544276 1_1:+5223321 Msa0002910:CDS
GCCAATGGATTGAGCGAAGG+TGG 0.546210 1_1:-5222239 None:intergenic
ACTTAAGAGGGTAATGCTGT+TGG 0.550014 1_1:-5222948 None:intergenic
TCCGAGATTGTTAGTGCCTG+CGG 0.554277 1_1:+5222520 Msa0002910:CDS
CATCTCCACTACTTTCCTGT+GGG 0.557649 1_1:-5223296 None:intergenic
CTGAAAGAACCAACGAGAGA+TGG 0.561073 1_1:-5220737 Msa0002900:intron
AGAACCAACGAGAGATGGTA+AGG 0.566590 1_1:-5220732 Msa0002900:intron
TGAAGTTCCCTGTCCGTGCA+CGG 0.571256 1_1:+5222984 Msa0002910:CDS
TTTGGACCTGCACGCCATTG+TGG 0.572595 1_1:-5223160 None:intergenic
ACTTGCTCCGTGCACGGACA+GGG 0.574860 1_1:-5222991 None:intergenic
TACGAGACTTGCTCCGTGCA+CGG 0.578328 1_1:-5222997 None:intergenic
CGTGCCTTACCATCTCTCGT+TGG 0.580045 1_1:+5220728 None:intergenic
ATGATGACATTTCCTCCCAA+TGG 0.580868 1_1:-5223119 None:intergenic
ACAGGAAAGTAGTGGAGATG+AGG 0.582547 1_1:+5223299 Msa0002910:CDS
ACCGCAGGCACTAACAATCT+CGG 0.585134 1_1:-5222521 None:intergenic
TGAATTGATGGGATCATCCA+GGG 0.600445 1_1:-5222261 None:intergenic
GCATCAGCAAGGCCAAGGCG+AGG 0.601336 1_1:-5223025 None:intergenic
GACACTTTGTAATGCATGTG+GGG 0.601729 1_1:+5223192 Msa0002910:CDS
AAGACACTTTGTAATGCATG+TGG 0.602236 1_1:+5223190 Msa0002910:CDS
TAATTCCCACAGGAAAGTAG+TGG 0.604924 1_1:+5223291 Msa0002910:CDS
GAGGTCGTCCACAGAACCAA+GGG 0.608143 1_1:-5222129 None:intergenic
AGACACTTTGTAATGCATGT+GGG 0.608576 1_1:+5223191 Msa0002910:CDS
CTTACAGAAAGTGAATTGAT+GGG 0.611737 1_1:-5222272 None:intergenic
GTGATTGTAGCCACTTAAGA+GGG 0.622729 1_1:-5222960 None:intergenic
ACATGCATTCTAATTCCCAC+AGG 0.630282 1_1:+5223281 Msa0002910:CDS
AACAAACCCCAACCATTGGG+AGG 0.635541 1_1:+5223107 Msa0002910:CDS
AGGGCCAATGGATTGAGCGA+AGG 0.639769 1_1:-5222242 None:intergenic
AGAGGTCGTCCACAGAACCA+AGG 0.642044 1_1:-5222130 None:intergenic
GGGAGGAAATGTCATCATTG+TGG 0.646984 1_1:+5223124 Msa0002910:CDS
CCTCAAACCTACAATTCCCG+TGG 0.649034 1_1:+5223047 Msa0002910:CDS
GACGGTATTCAGGTACAAGT+CGG 0.649278 1_1:-5223231 None:intergenic
AAGTTGGGCTATTTGCAGGA+CGG 0.658089 1_1:-5223249 None:intergenic
TTTCTCATCTGACATAGGTG+AGG 0.665571 1_1:+5222157 Msa0002910:CDS
GTAGGTTTGAGGCATCAGCA+AGG 0.716777 1_1:-5223036 None:intergenic
TGTGCTGTTCTCGAGCAGTG+GGG 0.722420 1_1:-5222900 None:intergenic
TGTCAGATGAGAAATCCAAG+AGG 0.747403 1_1:-5222148 None:intergenic
GAGTTGATGATACTCCACAA+TGG 0.801331 1_1:+5223146 Msa0002910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTATTATTTGTGAAG+TGG + chr1_1:5221008-5221027 Msa0002910:intron 15.0%
!! TTTATTATTATTTGTGAAGT+GGG + chr1_1:5221009-5221028 Msa0002910:intron 15.0%
!!! TTTGTTTAAGGTTTAATTTA+AGG + chr1_1:5221112-5221131 Msa0002910:intron 15.0%
!!! TTTTCTAATCATTTTCATAT+TGG + chr1_1:5221194-5221213 Msa0002910:intron 15.0%
!!! AATTTTATTGTGTTTATGAA+TGG + chr1_1:5221763-5221782 Msa0002910:intron 15.0%
!!! TTTTGTAATATTTGTTTGTA+CGG + chr1_1:5222055-5222074 Msa0002910:intron 15.0%
!! AAAAAACATAGCAAAGATTA+AGG - chr1_1:5220888-5220907 Msa0002900:CDS 20.0%
!! ATTTGAATATGCTGAAAATT+TGG + chr1_1:5220984-5221003 Msa0002910:intron 20.0%
!! TATTGAAGGAATAATGAAAT+GGG + chr1_1:5221040-5221059 Msa0002910:intron 20.0%
!! AACAAAGAAAAACAAGAAAA+TGG - chr1_1:5221098-5221117 None:intergenic 20.0%
!!! TTCTTGTTTTTCTTTGTTTA+AGG + chr1_1:5221100-5221119 Msa0002910:intron 20.0%
!! AAAGATTAAAGAACTTCATT+TGG - chr1_1:5221341-5221360 None:intergenic 20.0%
!!! GTTGTTTTTATAAGCTATAT+TGG + chr1_1:5221498-5221517 Msa0002910:intron 20.0%
!! AACAATGAATTAAAACTAGA+GGG - chr1_1:5221831-5221850 None:intergenic 20.0%
!!! TGAAAATTTCATTTTAGGTT+TGG - chr1_1:5221972-5221991 None:intergenic 20.0%
!!! AAACCTAAAATGAAATTTTC+AGG + chr1_1:5221971-5221990 Msa0002910:intron 20.0%
!!! AATCCTGAAAATTTCATTTT+AGG - chr1_1:5221977-5221996 None:intergenic 20.0%
!! ACAGATAAAAATAACAGTAT+AGG - chr1_1:5222086-5222105 None:intergenic 20.0%
!!! AAGTATTTTGAAAAGGTTAA+TGG - chr1_1:5222307-5222326 None:intergenic 20.0%
!! GTCATTGATAAAATGAAAAA+TGG + chr1_1:5223351-5223370 Msa0002910:three_prime_UTR 20.0%
!!! TAGATTAAAATTTTGCAGTT+AGG + chr1_1:5223509-5223528 Msa0002910:three_prime_UTR 20.0%
!!! AGATTAAAATTTTGCAGTTA+GGG + chr1_1:5223510-5223529 Msa0002910:three_prime_UTR 20.0%
! AAAAACAAGCAAGAAAAAGA+GGG - chr1_1:5220931-5220950 None:intergenic 25.0%
! TAAAAACAAGCAAGAAAAAG+AGG - chr1_1:5220932-5220951 None:intergenic 25.0%
! TAGAGGAAAATTAAACATAG+TGG - chr1_1:5220960-5220979 None:intergenic 25.0%
! AGCATATTCAAATAACATAG+AGG - chr1_1:5220977-5220996 None:intergenic 25.0%
! GTATTGAAGGAATAATGAAA+TGG + chr1_1:5221039-5221058 Msa0002910:intron 25.0%
! AAAGAACAGAAACAATGAAT+TGG - chr1_1:5221310-5221329 None:intergenic 25.0%
! TGTAAAATCGTATACTTTAC+TGG + chr1_1:5221644-5221663 Msa0002910:intron 25.0%
!!! ATACTTTACTGGTTTTTGTA+TGG + chr1_1:5221655-5221674 Msa0002910:intron 25.0%
! AAAAGTAACTCAAGTAATGA+AGG - chr1_1:5221710-5221729 None:intergenic 25.0%
! TAATGTAAAGAACTTATTCC+AGG - chr1_1:5221801-5221820 None:intergenic 25.0%
! GAACAATGAATTAAAACTAG+AGG - chr1_1:5221832-5221851 None:intergenic 25.0%
!! GTTTTCATATGTTGTAATCT+TGG + chr1_1:5221876-5221895 Msa0002910:intron 25.0%
! TTACAAAATAGTGAAAAGCA+TGG - chr1_1:5222043-5222062 None:intergenic 25.0%
!!! TGTATATTTGTTTTCAGAGA+TGG + chr1_1:5222103-5222122 Msa0002910:intron 25.0%
!!! ATCAAGCAAGTATTTTGAAA+AGG - chr1_1:5222314-5222333 None:intergenic 25.0%
! AAGGAAAACAAGTAAAAATC+TGG - chr1_1:5222472-5222491 None:intergenic 25.0%
!!! TCCTTTTAGAATCAATGTAT+TGG + chr1_1:5222487-5222506 Msa0002910:intron 25.0%
!!! ATTCCTTTTTTCCATACTTA+TGG + chr1_1:5222718-5222737 Msa0002910:intron 25.0%
!!! TTCCTTTTTTCCATACTTAT+GGG + chr1_1:5222719-5222738 Msa0002910:intron 25.0%
!! AAAACCAAAATCCTAACTTT+TGG - chr1_1:5223408-5223427 None:intergenic 25.0%
! TTGACAAGCAAAATGATTTA+AGG - chr1_1:5223539-5223558 None:intergenic 25.0%
! TAAGTTTTGCGAGACTATTA+GGG - chr1_1:5221589-5221608 None:intergenic 30.0%
! TACGATTTTACACATCGTAT+TGG - chr1_1:5221636-5221655 None:intergenic 30.0%
!!! GAATGGTGTTTTATTTTACC+TGG + chr1_1:5221780-5221799 Msa0002910:intron 30.0%
CCTTGTGATCGTTATTTATT+AGG - chr1_1:5221943-5221962 None:intergenic 30.0%
CCTAATAAATAACGATCACA+AGG + chr1_1:5221940-5221959 Msa0002910:intron 30.0%
!! ATATTTGTTTTCAGAGATGG+AGG + chr1_1:5222106-5222125 Msa0002910:intron 30.0%
CTTACAGAAAGTGAATTGAT+GGG - chr1_1:5222275-5222294 None:intergenic 30.0%
ACTTACAGAAAGTGAATTGA+TGG - chr1_1:5222276-5222295 None:intergenic 30.0%
TTCACTTTCTGTAAGTTTGA+CGG + chr1_1:5222280-5222299 Msa0002910:intron 30.0%
!!! TATCTAGTTTTGACTTGTTG+AGG + chr1_1:5222338-5222357 Msa0002910:intron 30.0%
! CACTTGCTAATATTCTACTT+TGG - chr1_1:5222446-5222465 None:intergenic 30.0%
!! GCCAATACATTGATTCTAAA+AGG - chr1_1:5222491-5222510 None:intergenic 30.0%
! AGAATCAATGTATTGGCATT+TGG + chr1_1:5222494-5222513 Msa0002910:intron 30.0%
AACCCATAAGTATGGAAAAA+AGG - chr1_1:5222724-5222743 None:intergenic 30.0%
ATTTGATGCTTCAAAAGATC+CGG - chr1_1:5222875-5222894 None:intergenic 30.0%
!!! CACTTTTTCTTTGGAAGTTT+TGG - chr1_1:5223086-5223105 None:intergenic 30.0%
!!! GGTTTGTTTCACTTTTTCTT+TGG - chr1_1:5223095-5223114 None:intergenic 30.0%
!! ATTTTAGTCCATTTTTGCTG+TGG + chr1_1:5223459-5223478 Msa0002910:three_prime_UTR 30.0%
!! TTTTAGTCCATTTTTGCTGT+GGG + chr1_1:5223460-5223479 Msa0002910:three_prime_UTR 30.0%
! TTAAAATTTTGCAGTTAGGG+AGG + chr1_1:5223513-5223532 Msa0002910:three_prime_UTR 30.0%
ATGTACTTTGATTGAGGATT+AGG + chr1_1:5223612-5223631 Msa0002910:three_prime_UTR 30.0%
TGTACTTTGATTGAGGATTA+GGG + chr1_1:5223613-5223632 Msa0002910:three_prime_UTR 30.0%
GTTCAGATAAGCATAATGAA+TGG - chr1_1:5223668-5223687 None:intergenic 30.0%
TGCTTATCTGAACTAATTCT+AGG + chr1_1:5223675-5223694 Msa0002910:three_prime_UTR 30.0%
!! AATTCTAGGTTGATGAATAG+TGG + chr1_1:5223689-5223708 Msa0002910:three_prime_UTR 30.0%
! GTGGTGTAGTAAAATTTTTG+AGG + chr1_1:5223708-5223727 Msa0002910:three_prime_UTR 30.0%
! TCAGTCTCTCAATTCTCTTT+TGG + chr1_1:5220756-5220775 Msa0002910:five_prime_UTR 35.0%
! CAGTCTCTCAATTCTCTTTT+GGG + chr1_1:5220757-5220776 Msa0002910:five_prime_UTR 35.0%
! AGTCTCTCAATTCTCTTTTG+GGG + chr1_1:5220758-5220777 Msa0002910:five_prime_UTR 35.0%
GAAGGAATAATGAAATGGGT+TGG + chr1_1:5221044-5221063 Msa0002910:intron 35.0%
AATGGTGAAGAGAGTCAAAA+TGG - chr1_1:5221080-5221099 None:intergenic 35.0%
TGTCTTAGTGAGTGAAAATG+TGG + chr1_1:5221231-5221250 Msa0002910:intron 35.0%
AATAAGCCGTTTATCCAAAC+AGG - chr1_1:5221380-5221399 None:intergenic 35.0%
! GTAAGTTTTGCGAGACTATT+AGG - chr1_1:5221590-5221609 None:intergenic 35.0%
TTGGATTTCTCATCTGACAT+AGG + chr1_1:5222152-5222171 Msa0002910:CDS 35.0%
TACTTGTGATCACTGTTTCA+AGG + chr1_1:5222583-5222602 Msa0002910:intron 35.0%
TATTCACACTGAGAAAGATG+CGG - chr1_1:5222625-5222644 None:intergenic 35.0%
CTTCACATAACCCATAAGTA+TGG - chr1_1:5222732-5222751 None:intergenic 35.0%
TCCTGGAACATGAAAATAAG+CGG - chr1_1:5222764-5222783 None:intergenic 35.0%
TCAACAAATGTTTCAACAGC+CGG - chr1_1:5222836-5222855 None:intergenic 35.0%
TGAATTGTCGTGTATTCAAC+CGG + chr1_1:5222853-5222872 Msa0002910:CDS 35.0%
!! TCTTTGGAAGTTTTGGTACT+CGG - chr1_1:5223079-5223098 None:intergenic 35.0%
! CATGCATTACAAAGTGTCTT+TGG - chr1_1:5223190-5223209 None:intergenic 35.0%
AAGACACTTTGTAATGCATG+TGG + chr1_1:5223190-5223209 Msa0002910:CDS 35.0%
AGACACTTTGTAATGCATGT+GGG + chr1_1:5223191-5223210 Msa0002910:CDS 35.0%
CATTTAGCATGCCAAAAGTT+AGG + chr1_1:5223394-5223413 Msa0002910:three_prime_UTR 35.0%
! CATGCCAAAAGTTAGGATTT+TGG + chr1_1:5223401-5223420 Msa0002910:three_prime_UTR 35.0%
AAAACATCCCACAGCAAAAA+TGG - chr1_1:5223470-5223489 None:intergenic 35.0%
! TCATTTGACTGTTTTCATGC+TGG + chr1_1:5223585-5223604 Msa0002910:three_prime_UTR 35.0%
GGTAACATGTACTTTGATTG+AGG + chr1_1:5223606-5223625 Msa0002910:three_prime_UTR 35.0%
AGTACACAAACGTGAAAAGC+TGG + chr1_1:5220834-5220853 Msa0002910:five_prime_UTR 40.0%
CAAGCAAGAAAAAGAGGGAA+AGG - chr1_1:5220926-5220945 None:intergenic 40.0%
! AGTGGGTGTGATAGTATTGA+AGG + chr1_1:5221026-5221045 Msa0002910:intron 40.0%
!! GGAATAATGAAATGGGTTGG+TGG + chr1_1:5221047-5221066 Msa0002910:intron 40.0%
!! TAATCTTTTCTGACTAGCCG+CGG + chr1_1:5221353-5221372 Msa0002910:intron 40.0%
TGTCAGATGAGAAATCCAAG+AGG - chr1_1:5222151-5222170 None:intergenic 40.0%
TTTCTCATCTGACATAGGTG+AGG + chr1_1:5222157-5222176 Msa0002910:CDS 40.0%
AGTGAAGGAAAGGCTTTCTT+AGG - chr1_1:5222200-5222219 None:intergenic 40.0%
!! TTCTGGTTTGAGTGAAGGAA+AGG - chr1_1:5222210-5222229 None:intergenic 40.0%
TGAATTGATGGGATCATCCA+GGG - chr1_1:5222264-5222283 None:intergenic 40.0%
GTTTCAAGGTTTCCATGTCA+TGG + chr1_1:5222597-5222616 Msa0002910:intron 40.0%
GTCAACGACACAACAAACAT+TGG - chr1_1:5222661-5222680 None:intergenic 40.0%
!! GCTTATTTTCATGTTCCAGG+AGG + chr1_1:5222763-5222782 Msa0002910:intron 40.0%
! TATTTTCATGTTCCAGGAGG+AGG + chr1_1:5222766-5222785 Msa0002910:intron 40.0%
GTCCAACAAAGATGCATTTC+CGG + chr1_1:5222814-5222833 Msa0002910:CDS 40.0%
ACTTAAGAGGGTAATGCTGT+TGG - chr1_1:5222951-5222970 None:intergenic 40.0%
AACAGCATTACCCTCTTAAG+TGG + chr1_1:5222950-5222969 Msa0002910:CDS 40.0%
GTGATTGTAGCCACTTAAGA+GGG - chr1_1:5222963-5222982 None:intergenic 40.0%
TGTGATTGTAGCCACTTAAG+AGG - chr1_1:5222964-5222983 None:intergenic 40.0%
TGAAACAAACCCCAACCATT+GGG + chr1_1:5223104-5223123 Msa0002910:CDS 40.0%
ATGATGACATTTCCTCCCAA+TGG - chr1_1:5223122-5223141 None:intergenic 40.0%
!! GAGTTGATGATACTCCACAA+TGG + chr1_1:5223146-5223165 Msa0002910:CDS 40.0%
! CAAAGTGTCTTTGGACCATT+TGG - chr1_1:5223181-5223200 None:intergenic 40.0%
GACACTTTGTAATGCATGTG+GGG + chr1_1:5223192-5223211 Msa0002910:CDS 40.0%
TTTGTAATGCATGTGGGGTT+AGG + chr1_1:5223197-5223216 Msa0002910:CDS 40.0%
ACATGCATTCTAATTCCCAC+AGG + chr1_1:5223281-5223300 Msa0002910:CDS 40.0%
TAATTCCCACAGGAAAGTAG+TGG + chr1_1:5223291-5223310 Msa0002910:CDS 40.0%
!! TTGCTGTGGGATGTTTTAGA+TGG + chr1_1:5223473-5223492 Msa0002910:three_prime_UTR 40.0%
!! TGCTGTGGGATGTTTTAGAT+GGG + chr1_1:5223474-5223493 Msa0002910:three_prime_UTR 40.0%
CTGAAAGAACCAACGAGAGA+TGG - chr1_1:5220740-5220759 Msa0002900:CDS 45.0%
!! AATCTTTTCTGACTAGCCGC+GGG + chr1_1:5221354-5221373 Msa0002910:intron 45.0%
!! CTGCATTCTGGTTTGAGTGA+AGG - chr1_1:5222215-5222234 None:intergenic 45.0%
GTGAATTGATGGGATCATCC+AGG - chr1_1:5222265-5222284 None:intergenic 45.0%
AAAGATGCGGTTCCATGACA+TGG - chr1_1:5222612-5222631 None:intergenic 45.0%
! TCGTTGACTAGTTGCATCAG+TGG + chr1_1:5222673-5222692 Msa0002910:intron 45.0%
!! GCCGCTTATTTTCATGTTCC+AGG + chr1_1:5222760-5222779 Msa0002910:intron 45.0%
TTGCAGAAGAGGAGCTTGAA+TGG + chr1_1:5222789-5222808 Msa0002910:CDS 45.0%
AGCCGGAAATGCATCTTTGT+TGG - chr1_1:5222819-5222838 None:intergenic 45.0%
! GTTGTTTCCACGGGAATTGT+AGG - chr1_1:5223057-5223076 None:intergenic 45.0%
! TTGGTACTCGGTTGTTTCCA+CGG - chr1_1:5223067-5223086 None:intergenic 45.0%
GTGAAACAAACCCCAACCAT+TGG + chr1_1:5223103-5223122 Msa0002910:CDS 45.0%
ACATTTCCTCCCAATGGTTG+GGG - chr1_1:5223116-5223135 None:intergenic 45.0%
GACATTTCCTCCCAATGGTT+GGG - chr1_1:5223117-5223136 None:intergenic 45.0%
TGACATTTCCTCCCAATGGT+TGG - chr1_1:5223118-5223137 None:intergenic 45.0%
GGGAGGAAATGTCATCATTG+TGG + chr1_1:5223124-5223143 Msa0002910:CDS 45.0%
TGTGGGGTTAGGTATAAGTC+TGG + chr1_1:5223208-5223227 Msa0002910:CDS 45.0%
GACGGTATTCAGGTACAAGT+CGG - chr1_1:5223234-5223253 None:intergenic 45.0%
CTATTTGCAGGACGGTATTC+AGG - chr1_1:5223244-5223263 None:intergenic 45.0%
AAGTTGGGCTATTTGCAGGA+CGG - chr1_1:5223252-5223271 None:intergenic 45.0%
CGAAAAGTTGGGCTATTTGC+AGG - chr1_1:5223256-5223275 None:intergenic 45.0%
GCATGTCACTGCGAAAAGTT+GGG - chr1_1:5223267-5223286 None:intergenic 45.0%
TGCATGTCACTGCGAAAAGT+TGG - chr1_1:5223268-5223287 None:intergenic 45.0%
CATCTCCACTACTTTCCTGT+GGG - chr1_1:5223299-5223318 None:intergenic 45.0%
TCATCTCCACTACTTTCCTG+TGG - chr1_1:5223300-5223319 None:intergenic 45.0%
! ACAGGAAAGTAGTGGAGATG+AGG + chr1_1:5223299-5223318 Msa0002910:CDS 45.0%
ATGAGGAAGCAGAAGGGAAT+GGG + chr1_1:5223316-5223335 Msa0002910:CDS 45.0%
TTGAGAGACAAGAGCCAGCT+AGG - chr1_1:5220812-5220831 Msa0002900:CDS 50.0%
! TTTTCAGAGATGGAGGCCCT+TGG + chr1_1:5222113-5222132 Msa0002910:intron 50.0%
GAAGGTGGATCACTGCATTC+TGG - chr1_1:5222227-5222246 None:intergenic 50.0%
TCCACCTTCGCTCAATCCAT+TGG + chr1_1:5222238-5222257 Msa0002910:CDS 50.0%
TCCGAGATTGTTAGTGCCTG+CGG + chr1_1:5222520-5222539 Msa0002910:CDS 50.0%
ACCGCAGGCACTAACAATCT+CGG - chr1_1:5222524-5222543 None:intergenic 50.0%
! GAATCTGTCATTTGCACCGC+AGG - chr1_1:5222539-5222558 None:intergenic 50.0%
! TTGTGCTGTTCTCGAGCAGT+GGG - chr1_1:5222904-5222923 None:intergenic 50.0%
! GTAGGTTTGAGGCATCAGCA+AGG - chr1_1:5223039-5223058 None:intergenic 50.0%
CCACGGGAATTGTAGGTTTG+AGG - chr1_1:5223050-5223069 None:intergenic 50.0%
CCTCAAACCTACAATTCCCG+TGG + chr1_1:5223047-5223066 Msa0002910:CDS 50.0%
! TGGTACTCGGTTGTTTCCAC+GGG - chr1_1:5223066-5223085 None:intergenic 50.0%
AACAAACCCCAACCATTGGG+AGG + chr1_1:5223107-5223126 Msa0002910:CDS 50.0%
AGTGGAGATGAGGAAGCAGA+AGG + chr1_1:5223309-5223328 Msa0002910:CDS 50.0%
GTGGAGATGAGGAAGCAGAA+GGG + chr1_1:5223310-5223329 Msa0002910:CDS 50.0%
GATGAGGAAGCAGAAGGGAA+TGG + chr1_1:5223315-5223334 Msa0002910:CDS 50.0%
GAAGCAGAAGGGAATGGGAA+CGG + chr1_1:5223321-5223340 Msa0002910:CDS 50.0%
AAGCAGAAGGGAATGGGAAC+GGG + chr1_1:5223322-5223341 Msa0002910:CDS 50.0%
AGCAGGCAAGAGAGAGAGAG+AGG - chr1_1:5220789-5220808 Msa0002900:CDS 55.0%
CGTTTATCCAAACAGGCCCG+CGG - chr1_1:5221373-5221392 None:intergenic 55.0%
CGCGGGCCTGTTTGGATAAA+CGG + chr1_1:5221371-5221390 Msa0002910:intron 55.0%
GAGGTCGTCCACAGAACCAA+GGG - chr1_1:5222132-5222151 None:intergenic 55.0%
AGAGGTCGTCCACAGAACCA+AGG - chr1_1:5222133-5222152 None:intergenic 55.0%
! TGGTTCTGTGGACGACCTCT+TGG + chr1_1:5222133-5222152 Msa0002910:CDS 55.0%
!! GCCAATGGATTGAGCGAAGG+TGG - chr1_1:5222242-5222261 None:intergenic 55.0%
!! AGGGCCAATGGATTGAGCGA+AGG - chr1_1:5222245-5222264 None:intergenic 55.0%
TTCGCTCAATCCATTGGCCC+TGG + chr1_1:5222244-5222263 Msa0002910:CDS 55.0%
ATGGGATCATCCAGGGCCAA+TGG - chr1_1:5222257-5222276 None:intergenic 55.0%
! TGTGCTGTTCTCGAGCAGTG+GGG - chr1_1:5222903-5222922 None:intergenic 55.0%
! CTTGTGCTGTTCTCGAGCAG+TGG - chr1_1:5222905-5222924 None:intergenic 55.0%
TGAAGTTCCCTGTCCGTGCA+CGG + chr1_1:5222984-5223003 Msa0002910:CDS 55.0%
! TACGAGACTTGCTCCGTGCA+CGG - chr1_1:5223000-5223019 None:intergenic 55.0%
! TTGAGGCATCAGCAAGGCCA+AGG - chr1_1:5223033-5223052 None:intergenic 55.0%
GATACTCCACAATGGCGTGC+AGG + chr1_1:5223154-5223173 Msa0002910:CDS 55.0%
TTTGGACCTGCACGCCATTG+TGG - chr1_1:5223163-5223182 None:intergenic 55.0%
CAATGGCGTGCAGGTCCAAA+TGG + chr1_1:5223163-5223182 Msa0002910:CDS 55.0%
CTCTCTTGCCTGCTCCTAGC+TGG + chr1_1:5220795-5220814 Msa0002910:five_prime_UTR 60.0%
GACAAGAGCCAGCTAGGAGC+AGG - chr1_1:5220806-5220825 Msa0002900:CDS 60.0%
!! GATGGAGGCCCTTGGTTCTG+TGG + chr1_1:5222121-5222140 Msa0002910:exon 60.0%
CCTCTTCTGCAACCTCCTCC+TGG - chr1_1:5222781-5222800 None:intergenic 60.0%
CCAGGAGGAGGTTGCAGAAG+AGG + chr1_1:5222778-5222797 Msa0002910:intron 60.0%
ACTTGCTCCGTGCACGGACA+GGG - chr1_1:5222994-5223013 None:intergenic 60.0%
TCTCGTAGCAAGCCTCGCCT+TGG + chr1_1:5223013-5223032 Msa0002910:CDS 60.0%
! TGACTAGCCGCGGGCCTGTT+TGG + chr1_1:5221363-5221382 Msa0002910:intron 65.0%
! GACTTGCTCCGTGCACGGAC+AGG - chr1_1:5222995-5223014 None:intergenic 65.0%
GCATCAGCAAGGCCAAGGCG+AGG - chr1_1:5223028-5223047 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 5220734 5223736 5220734 ID=Msa0002910;Name=Msa0002910
chr1_1 mRNA 5220734 5223736 5220734 ID=Msa0002910-mRNA-1;Parent=Msa0002910;Name=Msa0002910-mRNA-1;_AED=0.13;_eAED=0.13;_QI=124|0.33|0.75|0.75|0.66|0.75|4|388|266
chr1_1 exon 5220734 5220855 5220734 ID=Msa0002910-mRNA-1:exon:1576;Parent=Msa0002910-mRNA-1
chr1_1 exon 5222120 5222289 5222120 ID=Msa0002910-mRNA-1:exon:1577;Parent=Msa0002910-mRNA-1
chr1_1 exon 5222496 5222561 5222496 ID=Msa0002910-mRNA-1:exon:1578;Parent=Msa0002910-mRNA-1
chr1_1 exon 5222782 5223736 5222782 ID=Msa0002910-mRNA-1:exon:1579;Parent=Msa0002910-mRNA-1
chr1_1 five_prime_UTR 5220734 5220855 5220734 ID=Msa0002910-mRNA-1:five_prime_utr;Parent=Msa0002910-mRNA-1
chr1_1 five_prime_UTR 5222120 5222121 5222120 ID=Msa0002910-mRNA-1:five_prime_utr;Parent=Msa0002910-mRNA-1
chr1_1 CDS 5222122 5222289 5222122 ID=Msa0002910-mRNA-1:cds;Parent=Msa0002910-mRNA-1
chr1_1 CDS 5222496 5222561 5222496 ID=Msa0002910-mRNA-1:cds;Parent=Msa0002910-mRNA-1
chr1_1 CDS 5222782 5223348 5222782 ID=Msa0002910-mRNA-1:cds;Parent=Msa0002910-mRNA-1
chr1_1 three_prime_UTR 5223349 5223736 5223349 ID=Msa0002910-mRNA-1:three_prime_utr;Parent=Msa0002910-mRNA-1
Gene Sequence

>Msa0002910

ATGGAGGCCCTTGGTTCTGTGGACGACCTCTTGGATTTCTCATCTGACATAGGTGAGGATGATGATGATGACAAACCTAAGAAAGCCTTTCCTTCACTCAAACCAGAATGCAGTGATCCACCTTCGCTCAATCCATTGGCCCTGGATGATCCCATCAATTCACTTTCTAATCAATGTATTGGCATTTGGAATTCCGAGATTGTTAGTGCCTGCGGTGCAAATGACAGATTCTCTGAGGAGGTTGCAGAAGAGGAGCTTGAATGGCTGTCCAACAAAGATGCATTTCCGGCTGTTGAAACATTTGTTGAATTGTCGTGTATTCAACCGGATCTTTTGAAGCATCAAATGACAGCCCCACTGCTCGAGAACAGCACAAGCAGCAGCAACAGCAATAACAGTTCCAACAGCATTACCCTCTTAAGTGGCTACAATCACATGAAGTTCCCTGTCCGTGCACGGAGCAAGTCTCGTAGCAAGCCTCGCCTTGGCCTTGCTGATGCCTCAAACCTACAATTCCCGTGGAAACAACCGAGTACCAAAACTTCCAAAGAAAAAGTGAAACAAACCCCAACCATTGGGAGGAAATGTCATCATTGTGGAGTTGATGATACTCCACAATGGCGTGCAGGTCCAAATGGTCCAAAGACACTTTGTAATGCATGTGGGGTTAGGTATAAGTCTGGCCGACTTGTACCTGAATACCGTCCTGCAAATAGCCCAACTTTTCGCAGTGACATGCATTCTAATTCCCACAGGAAAGTAGTGGAGATGAGGAAGCAGAAGGGAATGGGAACGGGTTGA
Protein sequence

>Msa0002910

MEALGSVDDLLDFSSDIGEDDDDDKPKKAFPSLKPECSDPPSLNPLALDDPINSLSNQCIGIWNSEIVSACGANDRFSEEVAEEELEWLSNKDAFPAVETFVELSCIQPDLLKHQMTAPLLENSTSSSNSNNSSNSITLLSGYNHMKFPVRARSKSRSKPRLGLADASNLQFPWKQPSTKTSKEKVKQTPTIGRKCHHCGVDDTPQWRAGPNGPKTLCNACGVRYKSGRLVPEYRPANSPTFRSDMHSNSHRKVVEMRKQKGMGTG*