AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0003750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0003750 MtrunA17_Chr1g0155301 83.726 424 23 5 1 380 1 422 0.0 694
Msa0003750 MtrunA17_Chr5g0404321 55.504 427 132 9 1 380 1 416 7.60e-155 442
Msa0003750 MtrunA17_Chr8g0373001 55.399 426 130 10 1 380 1 412 1.06e-148 426
Msa0003750 MtrunA17_Chr1g0156151 85.408 233 16 3 1 227 1 221 3.19e-131 375
Msa0003750 MtrunA17_Chr2g0278511 46.818 440 161 11 1 380 1 427 9.96e-124 363
Msa0003750 MtrunA17_Chr1g0201891 44.469 452 158 12 1 380 1 431 8.23e-121 356
Msa0003750 MtrunA17_Chr2g0286441 51.482 371 130 7 52 380 31 393 7.31e-120 352
Msa0003750 MtrunA17_Chr1g0196071 45.000 420 173 10 1 380 1 402 1.39e-111 332
Msa0003750 MtrunA17_Chr2g0315641 47.536 345 154 5 54 380 36 371 2.07e-103 310
Msa0003750 MtrunA17_Chr8g0373361 42.195 410 170 12 7 373 20 405 1.60e-91 281
Msa0003750 MtrunA17_Chr2g0315651 43.966 348 173 8 49 380 26 367 3.01e-84 260
Msa0003750 MtrunA17_Chr8g0373331 38.386 409 155 11 1 373 1 348 2.30e-77 242
Msa0003750 MtrunA17_Chr8g0373341 41.026 390 140 11 1 370 1 320 7.63e-68 217
Msa0003750 MtrunA17_Chr8g0373351 34.877 367 118 9 49 377 8 291 8.47e-49 166
Msa0003750 MtrunA17_Chr3g0091401 44.792 96 46 2 105 200 11 99 4.89e-17 76.3
Msa0003750 MtrunA17_Chr2g0334041 28.889 270 118 13 140 379 134 359 2.33e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0003750 AT1G47270.3 55.122 410 132 9 1 380 1 388 1.54e-141 408
Msa0003750 AT1G47270.2 55.122 410 132 9 1 380 25 412 3.19e-141 408
Msa0003750 AT1G47270.1 55.122 410 132 9 1 380 26 413 3.41e-141 408
Msa0003750 AT2G18280.2 51.208 414 147 9 1 380 1 393 1.38e-131 382
Msa0003750 AT2G18280.1 51.208 414 147 9 1 380 1 393 1.38e-131 382
Msa0003750 AT2G47900.2 46.411 418 174 7 1 380 1 406 9.59e-122 358
Msa0003750 AT2G47900.1 46.411 418 174 7 1 380 1 406 9.59e-122 358
Msa0003750 AT2G47900.3 46.301 419 174 8 1 380 1 407 4.04e-120 354
Msa0003750 AT1G25280.1 45.011 451 171 9 1 380 1 445 3.13e-118 350
Msa0003750 AT1G43640.1 41.986 443 180 10 1 380 1 429 1.94e-105 317
Msa0003750 AT5G18680.1 47.967 369 142 10 52 380 31 389 2.20e-104 313
Msa0003750 AT3G06380.1 46.092 371 153 7 47 380 20 380 3.53e-104 312
Msa0003750 AT1G53320.1 44.974 378 188 6 20 380 5 379 3.60e-103 310
Msa0003750 AT1G43640.2 44.565 368 140 8 72 380 1 363 4.35e-94 286
Msa0003750 AT5G18680.2 47.213 305 112 9 115 380 3 297 4.55e-81 250
Msa0003750 AT3G06380.2 45.578 294 114 6 123 380 5 288 6.49e-80 247
Msa0003750 AT1G76900.2 51.131 221 99 2 1 218 1 215 5.51e-68 221
Msa0003750 AT1G76900.2 65.455 110 22 1 287 380 346 455 1.33e-40 149
Msa0003750 AT1G76900.1 51.131 221 99 2 1 218 1 215 5.51e-68 221
Msa0003750 AT1G76900.1 65.455 110 22 1 287 380 346 455 1.33e-40 149
Msa0003750 AT1G76900.3 51.131 221 99 2 1 218 1 215 5.51e-68 221
Msa0003750 AT1G76900.3 65.455 110 22 1 287 380 346 455 1.33e-40 149
Msa0003750 AT1G25280.3 43.866 269 81 6 180 380 1 267 2.32e-60 196
Msa0003750 AT1G25280.2 43.866 269 81 6 180 380 1 267 2.32e-60 196
Msa0003750 AT1G61940.2 50.575 87 43 0 63 149 1 87 8.81e-25 102
Msa0003750 AT1G61940.1 52.459 61 29 0 63 123 1 61 2.77e-14 72.4

Find 105 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTATTTGCAGCTCTTTC+TGG 0.147891 1_1:-6293226 Msa0003750:intron
AAGCCAGAAGTTGTTGAATT+TGG 0.203153 1_1:-6292690 Msa0003750:CDS
GTGCCTCAGTTTGTAGATTA+TGG 0.215782 1_1:-6293866 Msa0003750:CDS
GTTCTGCAGCTGAAACATTT+TGG 0.237846 1_1:+6292511 None:intergenic
TTGAGGTTTGTGAAGATTAT+AGG 0.238744 1_1:+6294933 None:intergenic
GGACAGAACAAAACTTAATT+TGG 0.258161 1_1:+6295079 None:intergenic
CCCTTCTTGGACTGAAGATA+TGG 0.262463 1_1:+6292778 None:intergenic
GAGCCAAATTCAACAACTTC+TGG 0.280199 1_1:+6292687 None:intergenic
GTAGGAGCAGTTCCCCTTCT+TGG 0.289665 1_1:+6292765 None:intergenic
ATTACCTGCTTCAGTGATAT+AGG 0.293400 1_1:+6293786 None:intergenic
GATAAGTTGTGTATATTTGT+AGG 0.300528 1_1:+6294163 None:intergenic
TGCTGCAACTTGCAACTTGA+TGG 0.318405 1_1:+6292932 None:intergenic
TCCACATATAGTTTATATCT+TGG 0.326644 1_1:-6293598 Msa0003750:CDS
CACTGAATTGGATTGTCTCT+TGG 0.352418 1_1:+6293646 None:intergenic
CTCTTAATAAAACACTGAAT+TGG 0.367629 1_1:+6293634 None:intergenic
TCTAGAAAATGAGGAAATCT+TGG 0.369298 1_1:+6294807 None:intergenic
TTTGTCCAAGACATTGCCTT+TGG 0.372742 1_1:-6294885 Msa0003750:five_prime_UTR
GTGAGAGTGGATGGAGGTTG+AGG 0.390805 1_1:+6294916 None:intergenic
ATACTTACATTGGAAAGTTA+AGG 0.392067 1_1:-6293102 Msa0003750:intron
TTCATGCTCATTGTGGCAAT+TGG 0.398540 1_1:+6292728 None:intergenic
GTTGGTGCTGATAGTGATAT+TGG 0.400859 1_1:+6294014 None:intergenic
ATGTCTTGGACAAAACAAAA+AGG 0.404771 1_1:+6294894 None:intergenic
TTACCTGCTTCAGTGATATA+GGG 0.408155 1_1:+6293787 None:intergenic
CATTGCCTTTGGAGTATTTG+AGG 0.412801 1_1:-6294874 Msa0003750:five_prime_UTR
AGAGAGATGAAAGAGGGAAT+TGG 0.414626 1_1:-6294116 Msa0003750:CDS
ACCAAGTTCACGATCTTAGA+TGG 0.416091 1_1:-6292980 Msa0003750:CDS
AGGAAATCTTGGAGAGAACT+TGG 0.419492 1_1:+6294818 None:intergenic
CCTTCTTGGACTGAAGATAT+GGG 0.424206 1_1:+6292779 None:intergenic
CATCATCATCAGAGAGTCAA+TGG 0.430216 1_1:-6293980 Msa0003750:CDS
TTTGTTCTGTCCATGTGGTT+TGG 0.430653 1_1:-6295068 Msa0003750:five_prime_UTR
TGCAGTTGTTCATGCCATCT+TGG 0.442171 1_1:+6292615 None:intergenic
GATGATGATGATGGTGTTGT+TGG 0.443322 1_1:+6293996 None:intergenic
TTGTGTTGGATTATAGCTTA+AGG 0.446378 1_1:-6295187 Msa0003750:five_prime_UTR
ACCATCTAAGATCGTGAACT+TGG 0.451643 1_1:+6292979 None:intergenic
ATGTGGTTTGGTTGTAAAGA+AGG 0.456396 1_1:-6295056 Msa0003750:five_prime_UTR
GATATTGGTGAGGAACATTC+TGG 0.475287 1_1:+6294029 None:intergenic
GGGAACTGCTCCTACTCCTA+TGG 0.481990 1_1:-6292757 Msa0003750:CDS
ATTGGATTGTCTCTTGGAGC+AGG 0.482570 1_1:+6293652 None:intergenic
GGGACGGGTTACAGTGGCTT+CGG 0.485242 1_1:-6292571 Msa0003750:CDS
GTGTTTGAATTTCAAGGGAC+GGG 0.490017 1_1:-6292586 Msa0003750:CDS
ATTGTGAGAGAGATGAAAGA+GGG 0.490577 1_1:-6294122 Msa0003750:CDS
AGGAGTTATCATTCACAAGC+TGG 0.492801 1_1:+6292369 None:intergenic
TATTGTGAGAGAGATGAAAG+AGG 0.494687 1_1:-6294123 Msa0003750:CDS
AAGTTGCAAGTTGCAGCAAA+AGG 0.503666 1_1:-6292927 Msa0003750:intron
TGATATGAGCAAACTTCTCT+TGG 0.505017 1_1:-6293203 Msa0003750:CDS
TTATATCTTGGTCTAAGCCC+TGG 0.508714 1_1:-6293586 Msa0003750:intron
TTATGGAAAGACATAACAAA+GGG 0.511649 1_1:-6293849 Msa0003750:CDS
AGACACTTGAACAGTGTGGC+TGG 0.511733 1_1:-6293818 Msa0003750:CDS
CACAAAGAGGGGAGGGAATG+TGG 0.514278 1_1:+6294845 None:intergenic
ACTATTAGACATAATTCAAA+GGG 0.516941 1_1:-6293937 Msa0003750:CDS
ACCAAGATATAAACTATATG+TGG 0.517704 1_1:+6293597 None:intergenic
ATTGTACTCCGCATTCGCCT+TGG 0.518756 1_1:+6292812 None:intergenic
GGTGTTTGAATTTCAAGGGA+CGG 0.518832 1_1:-6292587 Msa0003750:CDS
CCCATATCTTCAGTCCAAGA+AGG 0.522731 1_1:-6292779 Msa0003750:CDS
CCATATCTTCAGTCCAAGAA+GGG 0.527340 1_1:-6292778 Msa0003750:CDS
ATACTCCAAAGGCAATGTCT+TGG 0.529963 1_1:+6294880 None:intergenic
AGAGCTAACAATACTTACAT+TGG 0.531671 1_1:-6293112 Msa0003750:CDS
TGGCAATTGGTAAATTCCAT+AGG 0.538026 1_1:+6292741 None:intergenic
AACTTTCAAAACAAAGCTTG+TGG 0.541276 1_1:+6294975 None:intergenic
TTTCCCTATATCACTGAAGC+AGG 0.541444 1_1:-6293790 Msa0003750:intron
ATGTTGGTGTTTGAATTTCA+AGG 0.542716 1_1:-6292592 Msa0003750:CDS
TTGGTAAATTCCATAGGAGT+AGG 0.543440 1_1:+6292747 None:intergenic
AGACAAGAGTCTAGAAAATG+AGG 0.552127 1_1:+6294798 None:intergenic
TACTATTAGACATAATTCAA+AGG 0.554134 1_1:-6293938 Msa0003750:CDS
ATTATGTCTAATAGTAACTC+AGG 0.562516 1_1:+6293945 None:intergenic
TGTTGGTGTTTGAATTTCAA+GGG 0.562671 1_1:-6292591 Msa0003750:CDS
ATCATCATCAGAGAGTCAAT+GGG 0.565599 1_1:-6293979 Msa0003750:CDS
GAACTTGGAAAGCACAAAGA+GGG 0.569713 1_1:+6294833 None:intergenic
CAATATCTTGCATGGTAGTG+TGG 0.570310 1_1:+6295207 None:intergenic
TACCATGCAAGATATTGTGT+TGG 0.578066 1_1:-6295201 Msa0003750:five_prime_UTR
GCTACAAGTGCTCGGCGAGA+TGG 0.582646 1_1:+6293891 None:intergenic
TTGCAGATTCATGCTCATTG+TGG 0.583392 1_1:+6292721 None:intergenic
TCTCTCACAATACTCTTGAA+AGG 0.583510 1_1:+6294134 None:intergenic
TTCTTTACAACCAAACCACA+TGG 0.588770 1_1:+6295058 None:intergenic
ATCCAACACAATATCTTGCA+TGG 0.599048 1_1:+6295199 None:intergenic
GGCATGAACAACTGCAATGT+TGG 0.599403 1_1:-6292608 Msa0003750:CDS
GAGAGGATATCGATAATCCA+TGG 0.599763 1_1:+6292440 None:intergenic
CTTCTTGGACTGAAGATATG+GGG 0.599973 1_1:+6292780 None:intergenic
CGAGAGGTCCAAGGCGAATG+CGG 0.601851 1_1:-6292820 Msa0003750:intron
ATTAGACATAATTCAAAGGG+TGG 0.608482 1_1:-6293934 Msa0003750:CDS
TGGAAAGCACAAAGAGGGGA+GGG 0.611473 1_1:+6294838 None:intergenic
TTCTTCGTACGAGAGGTCCA+AGG 0.616949 1_1:-6292829 Msa0003750:intron
ACTAAGCATATGCATAGACA+TGG 0.617362 1_1:-6294068 Msa0003750:CDS
AAGTACCTCAAATACTCCAA+AGG 0.627238 1_1:+6294869 None:intergenic
GCTGCTCAAACAGATTGCAA+AGG 0.630739 1_1:+6292404 None:intergenic
ATTATGGAAAGACATAACAA+AGG 0.634598 1_1:-6293850 Msa0003750:CDS
AGGCAAGTGATAAAAGTGCA+AGG 0.635649 1_1:-6295167 Msa0003750:five_prime_UTR
TGTGGTTTGGTTGTAAAGAA+GGG 0.635990 1_1:-6295055 Msa0003750:five_prime_UTR
ATTGGCAACATGTATAGAAG+AGG 0.636703 1_1:-6294098 Msa0003750:CDS
CGGGAAGGACATATTCACCA+TGG 0.637363 1_1:-6292457 Msa0003750:CDS
TGGAAGCTAGTGAAACCACT+TGG 0.639315 1_1:-6293914 Msa0003750:CDS
TTTCAAGGGACGGGTTACAG+TGG 0.641025 1_1:-6292577 Msa0003750:CDS
AGGCACAAGCTACAAGTGCT+CGG 0.645228 1_1:+6293883 None:intergenic
AGAACTTGGAAAGCACAAAG+AGG 0.645315 1_1:+6294832 None:intergenic
AACTTGGAAAGCACAAAGAG+GGG 0.645714 1_1:+6294834 None:intergenic
TTGGAAAGCACAAAGAGGGG+AGG 0.646454 1_1:+6294837 None:intergenic
CATATCTTCAGTCCAAGAAG+GGG 0.650598 1_1:-6292777 Msa0003750:CDS
TTTCCATAATCTACAAACTG+AGG 0.657977 1_1:+6293863 None:intergenic
ATGTCTAATAGTAACTCAGG+AGG 0.663267 1_1:+6293948 None:intergenic
AAGGCTTGAAAAGCAGAGAG+AGG 0.664732 1_1:+6292423 None:intergenic
TGCTCGGCGAGATGGCCAAG+TGG 0.681754 1_1:+6293899 None:intergenic
TGCTGATAGTGATATTGGTG+AGG 0.701371 1_1:+6294019 None:intergenic
GATGGAAGCGAAGAAACATG+TGG 0.713919 1_1:+6292950 None:intergenic
GGAAGCGAAGAAACATGTGG+AGG 0.757377 1_1:+6292953 None:intergenic
GTTAAGACACTTGAACAGTG+TGG 0.771470 1_1:-6293822 Msa0003750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAAATTCATATTAGATTA+AGG - chr1_1:6292881-6292900 Msa0003750:intron 15.0%
!!! TTTTGTAATAAATATTGTGA+TGG + chr1_1:6292927-6292946 None:intergenic 15.0%
!! ATCACAATATTTATTACAAA+AGG - chr1_1:6292926-6292945 Msa0003750:intron 15.0%
!! TCACAATATTTATTACAAAA+GGG - chr1_1:6292927-6292946 Msa0003750:intron 15.0%
!!! TTTTCTTTCACTTCTTTTTT+TGG - chr1_1:6292848-6292867 Msa0003750:intron 20.0%
!! GATATAGCAGTAAAAAAATA+AGG - chr1_1:6292949-6292968 Msa0003750:CDS 20.0%
!!! AACTGCCTTAATTATTTTTT+GGG + chr1_1:6293144-6293163 None:intergenic 20.0%
!!! AAACTGCCTTAATTATTTTT+TGG + chr1_1:6293145-6293164 None:intergenic 20.0%
!!! TTTTTTGAGGGAAATAATTA+AGG - chr1_1:6293168-6293187 Msa0003750:CDS 20.0%
!!! TTTTTCTTGTTCCTTTTTAT+AGG + chr1_1:6293217-6293236 None:intergenic 20.0%
!! TACTATTAGACATAATTCAA+AGG - chr1_1:6293651-6293670 Msa0003750:CDS 20.0%
!! ACTATTAGACATAATTCAAA+GGG - chr1_1:6293652-6293671 Msa0003750:CDS 20.0%
!!! TGTAATTTTTGTTTTAAGCT+TGG - chr1_1:6293857-6293876 Msa0003750:CDS 20.0%
!! ACATGAAATTATTCTCTATT+TGG - chr1_1:6294042-6294061 Msa0003750:CDS 20.0%
!!! TTTGGTAACAAAATTTTGTT+TGG - chr1_1:6294060-6294079 Msa0003750:CDS 20.0%
!! AAAAAAAAAAGCCTAAAAGA+AGG + chr1_1:6294156-6294175 None:intergenic 20.0%
!!! TTTTTTTAGTCTTTGAACTA+TGG - chr1_1:6294263-6294282 Msa0003750:intron 20.0%
!! AATAACACAATTCACAAATA+TGG + chr1_1:6294346-6294365 None:intergenic 20.0%
!!! CACAGAATTTTTAATATCAT+TGG - chr1_1:6294437-6294456 Msa0003750:intron 20.0%
!! TAATCATTAAGCAAAATAAC+AGG - chr1_1:6294571-6294590 Msa0003750:intron 20.0%
!! GAAAAAGTTATACTACAATT+TGG - chr1_1:6295103-6295122 Msa0003750:five_prime_UTR 20.0%
!! ATACTACAATTTGGAAAAAT+CGG - chr1_1:6295112-6295131 Msa0003750:five_prime_UTR 20.0%
! CAAGACCCAAAAAATAATTA+AGG - chr1_1:6293136-6293155 Msa0003750:CDS 25.0%
!!! AAGGCAGTTTTTTTTTTTTG+AGG - chr1_1:6293155-6293174 Msa0003750:CDS 25.0%
!!! AGGCAGTTTTTTTTTTTTGA+GGG - chr1_1:6293156-6293175 Msa0003750:CDS 25.0%
! GATAAGTTGTGTATATTTGT+AGG + chr1_1:6293429-6293448 None:intergenic 25.0%
! ATTATGTCTAATAGTAACTC+AGG + chr1_1:6293647-6293666 None:intergenic 25.0%
! ATTATGGAAAGACATAACAA+AGG - chr1_1:6293739-6293758 Msa0003750:intron 25.0%
! TTATGGAAAGACATAACAAA+GGG - chr1_1:6293740-6293759 Msa0003750:intron 25.0%
! CTCTTAATAAAACACTGAAT+TGG + chr1_1:6293958-6293977 None:intergenic 25.0%
!!! AGTGTTTTATTAAGAGAGAA+AGG - chr1_1:6293962-6293981 Msa0003750:CDS 25.0%
!!! GTGTTTTATTAAGAGAGAAA+GGG - chr1_1:6293963-6293982 Msa0003750:CDS 25.0%
! TCCACATATAGTTTATATCT+TGG - chr1_1:6293991-6294010 Msa0003750:CDS 25.0%
! ACCAAGATATAAACTATATG+TGG + chr1_1:6293995-6294014 None:intergenic 25.0%
!! AACTTGAATTACCTTCTTTT+AGG - chr1_1:6294142-6294161 Msa0003750:exon 25.0%
!!! ATTGATTTGTAAGTTTACTC+TGG + chr1_1:6294212-6294231 None:intergenic 25.0%
! GTTCAAAGACTAAAAAAAGA+AGG + chr1_1:6294261-6294280 None:intergenic 25.0%
! ATACTTACATTGGAAAGTTA+AGG - chr1_1:6294487-6294506 Msa0003750:intron 25.0%
!! AATAACAGGTCTAATTTTCT+CGG - chr1_1:6294585-6294604 Msa0003750:intron 25.0%
! TACTACAATTTGGAAAAATC+GGG - chr1_1:6295113-6295132 Msa0003750:five_prime_UTR 25.0%
TTGTGTTGGATTATAGCTTA+AGG - chr1_1:6292402-6292421 Msa0003750:CDS 30.0%
GGACAGAACAAAACTTAATT+TGG + chr1_1:6292513-6292532 None:intergenic 30.0%
AACTTTCAAAACAAAGCTTG+TGG + chr1_1:6292617-6292636 None:intergenic 30.0%
TTGAGGTTTGTGAAGATTAT+AGG + chr1_1:6292659-6292678 None:intergenic 30.0%
ATGTCTTGGACAAAACAAAA+AGG + chr1_1:6292698-6292717 None:intergenic 30.0%
TCTAGAAAATGAGGAAATCT+TGG + chr1_1:6292785-6292804 None:intergenic 30.0%
AGAAAAGCTCAAAAATTGAG+AGG + chr1_1:6292834-6292853 None:intergenic 30.0%
!! GCAGTTGAAAATTTTGAAAG+AGG - chr1_1:6293190-6293209 Msa0003750:CDS 30.0%
TTGAAAGAGGTCCTATAAAA+AGG - chr1_1:6293203-6293222 Msa0003750:CDS 30.0%
ACACTAACTACATTGAACTT+AGG - chr1_1:6293312-6293331 Msa0003750:intron 30.0%
GTGTTTGAAAGAGAGAAATT+AGG + chr1_1:6293383-6293402 None:intergenic 30.0%
TTTCTCTCTTTCAAACACAA+TGG - chr1_1:6293385-6293404 Msa0003750:intron 30.0%
ATTAGACATAATTCAAAGGG+TGG - chr1_1:6293655-6293674 Msa0003750:CDS 30.0%
TTTCCATAATCTACAAACTG+AGG + chr1_1:6293729-6293748 None:intergenic 30.0%
CACAAATCGAGAATAGTTAA+CGG + chr1_1:6294305-6294324 None:intergenic 30.0%
! TAACTATTCTCGATTTGTGA+AGG - chr1_1:6294306-6294325 Msa0003750:intron 30.0%
AGAGCTAACAATACTTACAT+TGG - chr1_1:6294477-6294496 Msa0003750:intron 30.0%
! AATCAATCCACATCTTTTCA+TGG - chr1_1:6294521-6294540 Msa0003750:intron 30.0%
!!! GCTTTGTTTTTCAAAATCAG+AGG + chr1_1:6294953-6294972 None:intergenic 30.0%
! ATGTTGGTGTTTGAATTTCA+AGG - chr1_1:6294997-6295016 Msa0003750:five_prime_UTR 30.0%
! TGTTGGTGTTTGAATTTCAA+GGG - chr1_1:6294998-6295017 Msa0003750:five_prime_UTR 30.0%
TACCATGCAAGATATTGTGT+TGG - chr1_1:6292388-6292407 Msa0003750:CDS 35.0%
ATCCAACACAATATCTTGCA+TGG + chr1_1:6292393-6292412 None:intergenic 35.0%
!!! TAAGTTTTGTTCTGTCCATG+TGG - chr1_1:6292516-6292535 Msa0003750:CDS 35.0%
TTCTTTACAACCAAACCACA+TGG + chr1_1:6292534-6292553 None:intergenic 35.0%
!! ATGTGGTTTGGTTGTAAAGA+AGG - chr1_1:6292533-6292552 Msa0003750:CDS 35.0%
!! TGTGGTTTGGTTGTAAAGAA+GGG - chr1_1:6292534-6292553 Msa0003750:CDS 35.0%
ACAAAACAAAAAGGTGAGAG+TGG + chr1_1:6292689-6292708 None:intergenic 35.0%
AAGTACCTCAAATACTCCAA+AGG + chr1_1:6292723-6292742 None:intergenic 35.0%
AGACAAGAGTCTAGAAAATG+AGG + chr1_1:6292794-6292813 None:intergenic 35.0%
! TTTTCTAGACTCTTGTCTCA+CGG - chr1_1:6292796-6292815 Msa0003750:CDS 35.0%
! CATTGATTACACAGCTTTCA+AGG - chr1_1:6293062-6293081 Msa0003750:intron 35.0%
AGAAAAATCTCAAGAGAAGC+AGG - chr1_1:6293230-6293249 Msa0003750:intron 35.0%
TCTCTCACAATACTCTTGAA+AGG + chr1_1:6293458-6293477 None:intergenic 35.0%
TATTGTGAGAGAGATGAAAG+AGG - chr1_1:6293466-6293485 Msa0003750:intron 35.0%
ATTGTGAGAGAGATGAAAGA+GGG - chr1_1:6293467-6293486 Msa0003750:intron 35.0%
ATTGGCAACATGTATAGAAG+AGG - chr1_1:6293491-6293510 Msa0003750:intron 35.0%
ACTAAGCATATGCATAGACA+TGG - chr1_1:6293521-6293540 Msa0003750:intron 35.0%
ATCATCATCAGAGAGTCAAT+GGG - chr1_1:6293610-6293629 Msa0003750:CDS 35.0%
ATGTCTAATAGTAACTCAGG+AGG + chr1_1:6293644-6293663 None:intergenic 35.0%
TTACCTGCTTCAGTGATATA+GGG + chr1_1:6293805-6293824 None:intergenic 35.0%
ATTACCTGCTTCAGTGATAT+AGG + chr1_1:6293806-6293825 None:intergenic 35.0%
!! GTTTTAAGCTTGGAAGAACA+TGG - chr1_1:6293867-6293886 Msa0003750:CDS 35.0%
ATTGTATTTGCAGCTCTTTC+TGG - chr1_1:6294363-6294382 Msa0003750:intron 35.0%
TGATATGAGCAAACTTCTCT+TGG - chr1_1:6294386-6294405 Msa0003750:intron 35.0%
! GAAAAGATGTGGATTGATTG+AGG + chr1_1:6294520-6294539 None:intergenic 35.0%
GCATTAACCATGAAAAGATG+TGG + chr1_1:6294531-6294550 None:intergenic 35.0%
TTGGAAGTAGTTGCTTCATA+TGG + chr1_1:6294689-6294708 None:intergenic 35.0%
ATATGAAGCAACTACTTCCA+AGG - chr1_1:6294688-6294707 Msa0003750:intron 35.0%
TATGAAGCAACTACTTCCAA+GGG - chr1_1:6294689-6294708 Msa0003750:intron 35.0%
!! GTTGCTACTTTATAGTTAGC+AGG + chr1_1:6294720-6294739 None:intergenic 35.0%
TTGGTAAATTCCATAGGAGT+AGG + chr1_1:6294845-6294864 None:intergenic 35.0%
TGGCAATTGGTAAATTCCAT+AGG + chr1_1:6294851-6294870 None:intergenic 35.0%
AAGCCAGAAGTTGTTGAATT+TGG - chr1_1:6294899-6294918 Msa0003750:five_prime_UTR 35.0%
! TGAAAAACAAAGCACCAAGA+TGG - chr1_1:6294960-6294979 Msa0003750:five_prime_UTR 35.0%
ACAATTTGGAAAAATCGGGA+AGG - chr1_1:6295117-6295136 Msa0003750:five_prime_UTR 35.0%
! CAATATCTTGCATGGTAGTG+TGG + chr1_1:6292385-6292404 None:intergenic 40.0%
! AGGCAAGTGATAAAAGTGCA+AGG - chr1_1:6292422-6292441 Msa0003750:CDS 40.0%
TTTGTTCTGTCCATGTGGTT+TGG - chr1_1:6292521-6292540 Msa0003750:CDS 40.0%
AACAAAAAGGTGAGAGTGGA+TGG + chr1_1:6292685-6292704 None:intergenic 40.0%
TTTGTCCAAGACATTGCCTT+TGG - chr1_1:6292704-6292723 Msa0003750:CDS 40.0%
ATACTCCAAAGGCAATGTCT+TGG + chr1_1:6292712-6292731 None:intergenic 40.0%
!! CATTGCCTTTGGAGTATTTG+AGG - chr1_1:6292715-6292734 Msa0003750:CDS 40.0%
! AACTTGGAAAGCACAAAGAG+GGG + chr1_1:6292758-6292777 None:intergenic 40.0%
! GAACTTGGAAAGCACAAAGA+GGG + chr1_1:6292759-6292778 None:intergenic 40.0%
! AGAACTTGGAAAGCACAAAG+AGG + chr1_1:6292760-6292779 None:intergenic 40.0%
AGGAAATCTTGGAGAGAACT+TGG + chr1_1:6292774-6292793 None:intergenic 40.0%
AAAATGTTAACGAGTGCCTC+CGG - chr1_1:6292972-6292991 Msa0003750:CDS 40.0%
AGAGAGATGAAAGAGGGAAT+TGG - chr1_1:6293473-6293492 Msa0003750:intron 40.0%
GATATTGGTGAGGAACATTC+TGG + chr1_1:6293563-6293582 None:intergenic 40.0%
! TGCTGATAGTGATATTGGTG+AGG + chr1_1:6293573-6293592 None:intergenic 40.0%
!! GTTGGTGCTGATAGTGATAT+TGG + chr1_1:6293578-6293597 None:intergenic 40.0%
!! GATGATGATGATGGTGTTGT+TGG + chr1_1:6293596-6293615 None:intergenic 40.0%
! CTCTCTGATGATGATGATGA+TGG + chr1_1:6293605-6293624 None:intergenic 40.0%
CATCATCATCAGAGAGTCAA+TGG - chr1_1:6293609-6293628 Msa0003750:CDS 40.0%
GTGCCTCAGTTTGTAGATTA+TGG - chr1_1:6293723-6293742 Msa0003750:intron 40.0%
GTTAAGACACTTGAACAGTG+TGG - chr1_1:6293767-6293786 Msa0003750:intron 40.0%
TTTCCCTATATCACTGAAGC+AGG - chr1_1:6293799-6293818 Msa0003750:CDS 40.0%
CACTGAATTGGATTGTCTCT+TGG + chr1_1:6293946-6293965 None:intergenic 40.0%
TTATATCTTGGTCTAAGCCC+TGG - chr1_1:6294003-6294022 Msa0003750:CDS 40.0%
TGAGCACAACAATCTTACCA+GGG + chr1_1:6294023-6294042 None:intergenic 40.0%
ACCAAGTTCACGATCTTAGA+TGG - chr1_1:6294609-6294628 Msa0003750:intron 40.0%
! ACCATCTAAGATCGTGAACT+TGG + chr1_1:6294613-6294632 None:intergenic 40.0%
AAGTTGCAAGTTGCAGCAAA+AGG - chr1_1:6294662-6294681 Msa0003750:intron 40.0%
CTTCTTGGACTGAAGATATG+GGG + chr1_1:6294812-6294831 None:intergenic 40.0%
CCTTCTTGGACTGAAGATAT+GGG + chr1_1:6294813-6294832 None:intergenic 40.0%
CCATATCTTCAGTCCAAGAA+GGG - chr1_1:6294811-6294830 Msa0003750:five_prime_UTR 40.0%
CATATCTTCAGTCCAAGAAG+GGG - chr1_1:6294812-6294831 Msa0003750:five_prime_UTR 40.0%
TTCATGCTCATTGTGGCAAT+TGG + chr1_1:6294864-6294883 None:intergenic 40.0%
TTGCAGATTCATGCTCATTG+TGG + chr1_1:6294871-6294890 None:intergenic 40.0%
GAGCCAAATTCAACAACTTC+TGG + chr1_1:6294905-6294924 None:intergenic 40.0%
GGTGTTTGAATTTCAAGGGA+CGG - chr1_1:6295002-6295021 Msa0003750:five_prime_UTR 40.0%
GTGTTTGAATTTCAAGGGAC+GGG - chr1_1:6295003-6295022 Msa0003750:five_prime_UTR 40.0%
! GTTCTGCAGCTGAAACATTT+TGG + chr1_1:6295081-6295100 None:intergenic 40.0%
GAGAGGATATCGATAATCCA+TGG + chr1_1:6295152-6295171 None:intergenic 40.0%
AAAAAGGTGAGAGTGGATGG+AGG + chr1_1:6292682-6292701 None:intergenic 45.0%
AAATGTTAACGAGTGCCTCC+GGG - chr1_1:6292973-6292992 Msa0003750:CDS 45.0%
TGCTTCCTTAACTAGTGCCA+CGG - chr1_1:6293101-6293120 Msa0003750:intron 45.0%
TGGAAGCTAGTGAAACCACT+TGG - chr1_1:6293675-6293694 Msa0003750:intron 45.0%
ATTGGATTGTCTCTTGGAGC+AGG + chr1_1:6293940-6293959 None:intergenic 45.0%
GTGAGCACAACAATCTTACC+AGG + chr1_1:6294024-6294043 None:intergenic 45.0%
GATGGAAGCGAAGAAACATG+TGG + chr1_1:6294642-6294661 None:intergenic 45.0%
TGCTGCAACTTGCAACTTGA+TGG + chr1_1:6294660-6294679 None:intergenic 45.0%
CTCAATGTTCTTCGTACGAG+AGG - chr1_1:6294753-6294772 Msa0003750:intron 45.0%
CCCATATCTTCAGTCCAAGA+AGG - chr1_1:6294810-6294829 Msa0003750:five_prime_UTR 45.0%
CCCTTCTTGGACTGAAGATA+TGG + chr1_1:6294814-6294833 None:intergenic 45.0%
TGCAGTTGTTCATGCCATCT+TGG + chr1_1:6294977-6294996 None:intergenic 45.0%
GGCATGAACAACTGCAATGT+TGG - chr1_1:6294981-6295000 Msa0003750:five_prime_UTR 45.0%
!!! GCAGCTGAAACATTTTGGCT+AGG + chr1_1:6295076-6295095 None:intergenic 45.0%
AAGGCTTGAAAAGCAGAGAG+AGG + chr1_1:6295169-6295188 None:intergenic 45.0%
GCTGCTCAAACAGATTGCAA+AGG + chr1_1:6295188-6295207 None:intergenic 45.0%
! TGGAAAGCACAAAGAGGGGA+GGG + chr1_1:6292754-6292773 None:intergenic 50.0%
! TTGGAAAGCACAAAGAGGGG+AGG + chr1_1:6292755-6292774 None:intergenic 50.0%
AATGTTAACGAGTGCCTCCG+GGG - chr1_1:6292974-6292993 Msa0003750:CDS 50.0%
TCTTGTTAACCAGTGCTCCG+TGG + chr1_1:6293121-6293140 None:intergenic 50.0%
AGGCACAAGCTACAAGTGCT+CGG + chr1_1:6293709-6293728 None:intergenic 50.0%
AGACACTTGAACAGTGTGGC+TGG - chr1_1:6293771-6293790 Msa0003750:intron 50.0%
GGAAGCGAAGAAACATGTGG+AGG + chr1_1:6294639-6294658 None:intergenic 50.0%
TTCTTCGTACGAGAGGTCCA+AGG - chr1_1:6294760-6294779 Msa0003750:intron 50.0%
ATTGTACTCCGCATTCGCCT+TGG + chr1_1:6294780-6294799 None:intergenic 50.0%
!! TTTCAAGGGACGGGTTACAG+TGG - chr1_1:6295012-6295031 Msa0003750:five_prime_UTR 50.0%
CGGGAAGGACATATTCACCA+TGG - chr1_1:6295132-6295151 Msa0003750:five_prime_UTR 50.0%
GTGAGAGTGGATGGAGGTTG+AGG + chr1_1:6292676-6292695 None:intergenic 55.0%
CACAAAGAGGGGAGGGAATG+TGG + chr1_1:6292747-6292766 None:intergenic 55.0%
AGCTTCCTTAACCAGTGCCC+CGG + chr1_1:6292994-6293013 None:intergenic 55.0%
GTGCTCCGTGGCACTAGTTA+AGG + chr1_1:6293109-6293128 None:intergenic 55.0%
TAACTAGTGCCACGGAGCAC+TGG - chr1_1:6293109-6293128 Msa0003750:CDS 55.0%
TAGTTAGCAGGAGCAGCCCT+TGG + chr1_1:6294708-6294727 None:intergenic 55.0%
GTAGGAGCAGTTCCCCTTCT+TGG + chr1_1:6294827-6294846 None:intergenic 55.0%
GGGAACTGCTCCTACTCCTA+TGG - chr1_1:6294832-6294851 Msa0003750:five_prime_UTR 55.0%
TTCCTTAACCAGTGCCCCGG+AGG + chr1_1:6292991-6293010 None:intergenic 60.0%
GCTACAAGTGCTCGGCGAGA+TGG + chr1_1:6293701-6293720 None:intergenic 60.0%
CGAGAGGTCCAAGGCGAATG+CGG - chr1_1:6294769-6294788 Msa0003750:intron 60.0%
!! GGGACGGGTTACAGTGGCTT+CGG - chr1_1:6295018-6295037 Msa0003750:five_prime_UTR 60.0%
!! TGCCTCCGGGGCACTGGTTA+AGG - chr1_1:6292986-6293005 Msa0003750:CDS 65.0%
TGCTCGGCGAGATGGCCAAG+TGG + chr1_1:6293693-6293712 None:intergenic 65.0%
AACGAGTGCCTCCGGGGCAC+TGG - chr1_1:6292980-6292999 Msa0003750:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 6292377 6295234 6292377 ID=Msa0003750;Name=Msa0003750
chr1_1 mRNA 6292377 6295234 6292377 ID=Msa0003750-mRNA-1;Parent=Msa0003750;Name=Msa0003750-mRNA-1;_AED=0.30;_eAED=0.30;_QI=476|0.8|0.66|1|0.8|0.66|6|0|380
chr1_1 exon 6292377 6292838 6292377 ID=Msa0003750-mRNA-1:exon:2098;Parent=Msa0003750-mRNA-1
chr1_1 exon 6292928 6293018 6292928 ID=Msa0003750-mRNA-1:exon:2097;Parent=Msa0003750-mRNA-1
chr1_1 exon 6293103 6293235 6293103 ID=Msa0003750-mRNA-1:exon:2096;Parent=Msa0003750-mRNA-1
chr1_1 exon 6293587 6293674 6293587 ID=Msa0003750-mRNA-1:exon:2095;Parent=Msa0003750-mRNA-1
chr1_1 exon 6293791 6294194 6293791 ID=Msa0003750-mRNA-1:exon:2094;Parent=Msa0003750-mRNA-1
chr1_1 exon 6294794 6295234 6294794 ID=Msa0003750-mRNA-1:exon:2093;Parent=Msa0003750-mRNA-1
chr1_1 five_prime_UTR 6294794 6295234 6294794 ID=Msa0003750-mRNA-1:five_prime_utr;Parent=Msa0003750-mRNA-1
chr1_1 five_prime_UTR 6294160 6294194 6294160 ID=Msa0003750-mRNA-1:five_prime_utr;Parent=Msa0003750-mRNA-1
chr1_1 CDS 6293791 6294159 6293791 ID=Msa0003750-mRNA-1:cds;Parent=Msa0003750-mRNA-1
chr1_1 CDS 6293587 6293674 6293587 ID=Msa0003750-mRNA-1:cds;Parent=Msa0003750-mRNA-1
chr1_1 CDS 6293103 6293235 6293103 ID=Msa0003750-mRNA-1:cds;Parent=Msa0003750-mRNA-1
chr1_1 CDS 6292928 6293018 6292928 ID=Msa0003750-mRNA-1:cds;Parent=Msa0003750-mRNA-1
chr1_1 CDS 6292377 6292838 6292377 ID=Msa0003750-mRNA-1:cds;Parent=Msa0003750-mRNA-1
Gene Sequence

>Msa0003750

ATGCCTTTCAAGAGTATTGTGAGAGAGATGAAAGAGGGAATTGGCAACATGTATAGAAGAGGTGTAGAAACTAAGCATATGCATAGACATGGAAAATCTCACATAGCACCAGAATGTTCCTCACCAATATCACTATCAGCACCAACAACACCATCATCATCATCATCAGAGAGTCAATGGGCTAATTTACCTCCTGAGTTACTATTAGACATAATTCAAAGGGTGGAAGCTAGTGAAACCACTTGGCCATCTCGCCGAGCACTTGTAGCTTGTGCCTCAGTTTGTAGATTATGGAAAGACATAACAAAGGGTGTTGTTAAGACACTTGAACAGTGTGGCTGGATCACTTTCCCTATATCACTGAAGCAGCCTGCTCCAAGAGACAATCCAATTCAGTGTTTTATTAAGAGAGAAAGGGCTACTTCCACATATAGTTTATATCTTGGTCTAAGCCCTGCTCTTTCTGGTGATATGAGCAAACTTCTCTTGGCAGCAAAGAAGATTAGAAGAGCAACATGCACAGAATTTTTAATATCATTGGTTTCTGATGATTTCTCTAGAGCTAACAATACTTACATTGGAAAGTTAAGGTCTAATTTTCTCGGCACCAAGTTCACGATCTTAGATGGTCATCCTCCACATGTTTCTTCGCTTCCATCAAGTTGCAAGTTGCAGCAAAAGAGGTCCAAGGCGAATGCGGAGTACAATGCACTTAATCCCCATATCTTCAGTCCAAGAAGGGGAACTGCTCCTACTCCTATGGAATTTACCAATTGCCACAATGAGCATGAATCTGCAAAAGAAAAGAAGCCAGAAGTTGTTGAATTTGGCTCAACTTGCACTGATTTTGCAAGAGAGCCTCTGATTTTGAAAAACAAAGCACCAAGATGGCATGAACAACTGCAATGTTGGTGTTTGAATTTCAAGGGACGGGTTACAGTGGCTTCGGTGAAGAATTTTCAGTTTGTAGCAGTTGCAGAACCTAGCCAAAATGTTTCAGCTGCAGAACAAGAAAAAGTTATACTACAATTTGGAAAAATCGGGAAGGACATATTCACCATGGATTATCGATATCCTCTCTCTGCTTTTCAAGCCTTTGCAATCTGTTTGAGCAGCTTTGACACAAAACCAGCTTGTGAATGA
Protein sequence

>Msa0003750

MPFKSIVREMKEGIGNMYRRGVETKHMHRHGKSHIAPECSSPISLSAPTTPSSSSSESQWANLPPELLLDIIQRVEASETTWPSRRALVACASVCRLWKDITKGVVKTLEQCGWITFPISLKQPAPRDNPIQCFIKRERATSTYSLYLGLSPALSGDMSKLLLAAKKIRRATCTEFLISLVSDDFSRANNTYIGKLRSNFLGTKFTILDGHPPHVSSLPSSCKLQQKRSKANAEYNALNPHIFSPRRGTAPTPMEFTNCHNEHESAKEKKPEVVEFGSTCTDFAREPLILKNKAPRWHEQLQCWCLNFKGRVTVASVKNFQFVAVAEPSQNVSAAEQEKVILQFGKIGKDIFTMDYRYPLSAFQAFAICLSSFDTKPACE*