AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0007330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0007330 MtrunA17_Chr3g0092621 83.333 72 12 0 23 94 80 151 3.59e-38 125
Msa0007330 MtrunA17_Chr4g0055931 73.239 71 19 0 26 96 94 164 1.32e-36 125
Msa0007330 MtrunA17_Chr2g0331031 73.913 69 18 0 26 94 108 176 7.81e-36 124
Msa0007330 MtrunA17_Chr6g0488471 78.788 66 14 0 26 91 96 161 5.03e-35 120
Msa0007330 MtrunA17_Chr5g0415191 87.143 70 9 0 26 95 101 170 4.79e-34 119
Msa0007330 MtrunA17_Chr4g0059701 95.714 70 3 0 26 95 83 152 2.27e-32 114
Msa0007330 MtrunA17_Chr8g0389241 61.628 86 27 2 6 91 89 168 3.69e-32 113
Msa0007330 MtrunA17_Chr4g0062741 66.667 75 25 0 26 100 137 211 6.35e-32 113
Msa0007330 MtrunA17_Chr5g0429521 69.118 68 21 0 26 93 131 198 7.09e-31 110
Msa0007330 MtrunA17_Chr1g0162081 70.588 68 20 0 26 93 121 188 1.04e-30 110
Msa0007330 MtrunA17_Chr3g0104071 69.118 68 21 0 26 93 146 213 1.10e-30 110
Msa0007330 MtrunA17_Chr1g0210471 56.977 86 37 0 8 93 105 190 4.18e-30 107
Msa0007330 MtrunA17_Chr7g0241761 68.657 67 21 0 26 92 139 205 1.23e-29 107
Msa0007330 MtrunA17_Chr6g0452791 75.000 60 15 0 27 86 80 139 3.53e-27 97.8
Msa0007330 MtrunA17_Chr3g0135671 65.217 69 21 1 26 91 90 158 1.08e-26 98.2
Msa0007330 MtrunA17_Chr5g0436501 63.889 72 24 1 26 95 124 195 4.82e-26 97.4
Msa0007330 MtrunA17_Chr5g0409841 51.515 66 30 1 29 92 94 159 2.03e-19 79.3
Msa0007330 MtrunA17_Chr5g0440741 47.436 78 35 2 29 100 102 179 1.08e-18 78.2
Msa0007330 MtrunA17_Chr5g0440731 46.250 80 37 2 27 100 105 184 1.53e-18 77.8
Msa0007330 MtrunA17_Chr5g0409831 62.500 48 18 0 27 74 77 124 6.27e-17 72.8
Msa0007330 MtrunA17_Chr8g0365011 48.718 78 26 2 29 92 94 171 7.20e-17 73.2
Msa0007330 MtrunA17_Chr8g0364731 59.322 59 22 1 36 92 10 68 1.55e-16 70.9
Msa0007330 MtrunA17_Chr1g0192061 43.678 87 43 3 13 96 49 132 1.35e-15 68.2
Msa0007330 MtrunA17_Chr3g0113121 47.222 72 31 1 29 93 97 168 2.13e-13 63.2
Msa0007330 MtrunA17_Chr5g0432921 49.254 67 31 2 29 93 115 180 3.11e-13 62.8
Msa0007330 MtrunA17_Chr5g0432941 42.683 82 44 2 14 93 100 180 3.45e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0007330 AT5G47390.1 76.812 69 16 0 26 94 95 163 5.40e-37 128
Msa0007330 AT1G49010.1 73.529 68 18 0 26 93 133 200 5.75e-34 119
Msa0007330 AT1G70000.1 84.286 70 11 0 26 95 97 166 1.10e-30 109
Msa0007330 AT1G70000.2 84.286 70 11 0 26 95 97 166 1.10e-30 109
Msa0007330 AT5G61620.1 65.217 69 24 0 26 94 107 175 2.33e-30 109
Msa0007330 AT5G04760.1 69.118 68 21 0 26 93 98 165 4.05e-30 107
Msa0007330 AT5G08520.1 69.118 68 21 0 26 93 117 184 4.30e-30 108
Msa0007330 AT5G56840.1 68.116 69 21 1 26 93 90 158 4.61e-30 107
Msa0007330 AT2G38090.1 69.118 68 21 0 26 93 140 207 6.58e-30 108
Msa0007330 AT5G58900.1 69.118 68 21 0 26 93 140 207 8.71e-30 107
Msa0007330 AT3G16350.1 75.362 69 17 0 26 94 136 204 3.31e-29 108
Msa0007330 AT1G74840.2 85.714 70 10 0 26 95 95 164 3.26e-28 102
Msa0007330 AT1G19000.2 80.000 75 15 0 26 100 101 175 3.37e-28 103
Msa0007330 AT1G19000.1 80.000 75 15 0 26 100 101 175 3.37e-28 103
Msa0007330 AT1G74840.1 85.714 70 10 0 26 95 95 164 4.22e-28 102
Msa0007330 AT5G01200.1 64.706 68 24 0 26 93 145 212 7.70e-27 99.8
Msa0007330 AT5G05790.2 63.235 68 25 0 26 93 130 197 8.15e-26 97.1
Msa0007330 AT5G05790.1 63.235 68 25 0 26 93 130 197 8.15e-26 97.1
Msa0007330 AT3G11280.2 63.235 68 25 0 26 93 126 193 8.61e-26 96.7
Msa0007330 AT3G11280.1 63.235 68 25 0 26 93 126 193 8.61e-26 96.7
Msa0007330 AT4G09450.1 52.000 75 36 0 25 99 89 163 3.28e-22 86.3
Msa0007330 AT3G10580.2 58.209 67 26 1 26 92 94 158 4.84e-22 87.0
Msa0007330 AT3G10580.1 58.209 67 26 1 26 92 94 158 7.01e-22 87.0
Msa0007330 AT5G23650.1 58.209 67 28 0 26 92 119 185 8.23e-22 87.4
Msa0007330 AT3G10590.1 50.725 69 33 1 28 96 112 179 1.82e-18 76.6

Find 17 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCATTATGTTAGTCCTTT+AGG 0.224788 1_1:+10483349 Msa0007330:intron
TTGGTTGGATTGGAGAAAGT+GGG 0.463944 1_1:+10482921 Msa0007330:CDS
AGAAAGTGGGAAAAGGTGAT+TGG 0.482958 1_1:+10482934 Msa0007330:CDS
TTTGGTTGGATTGGAGAAAG+TGG 0.487039 1_1:+10482920 Msa0007330:CDS
GGTGGTGATATCAAAGAGAC+TGG 0.540239 1_1:-10483066 None:intergenic
GGATTGGAGAAAGTGGGAAA+AGG 0.562648 1_1:+10482927 Msa0007330:CDS
TATCAAAGAGACTGGATCTA+CGG 0.592657 1_1:-10483058 None:intergenic
TTGTGCTCTTCCTCAGTCCA+CGG 0.594809 1_1:-10482891 None:intergenic
GTGATTGGAGAGGAATCTCA+AGG 0.619264 1_1:+10482949 Msa0007330:CDS
GTGGGAAAAGGTGATTGGAG+AGG 0.624127 1_1:+10482939 Msa0007330:CDS
TGAATGGATCAGGGATTCCG+TGG 0.626840 1_1:+10482874 Msa0007330:intron
GGCGATTGAGATTACTTCGG+CGG 0.629813 1_1:-10483031 None:intergenic
GCATGACTTGCAACATGCGT+AGG 0.636946 1_1:-10482993 None:intergenic
CTTTGATATCACCACCGACA+CGG 0.638033 1_1:+10483073 Msa0007330:CDS
GACGGCGATTGAGATTACTT+CGG 0.641222 1_1:-10483034 None:intergenic
ATCAGGGATTCCGTGGACTG+AGG 0.660333 1_1:+10482881 Msa0007330:intron
AGAGACTGGATCTACGGCGA+CGG 0.684123 1_1:-10483052 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTAATTGAATACAAATTT+AGG - chr1_1:10483147-10483166 None:intergenic 15.0%
!! TAATCAATAAAGTAGTATTA+TGG + chr1_1:10483169-10483188 Msa0007330:intron 15.0%
!!! AATTTTCTCTGTTATTTTTT+TGG + chr1_1:10483211-10483230 Msa0007330:intron 15.0%
!!! ATTTTGTATTATAAACCTAA+AGG - chr1_1:10483367-10483386 None:intergenic 15.0%
!!! TTAAGTCATTCATTTTTAAA+TGG + chr1_1:10483440-10483459 Msa0007330:intron 15.0%
!! AATACTCTAAATTAAACATA+AGG - chr1_1:10484151-10484170 None:intergenic 15.0%
!! AGTATTTCTTATAATATGTA+TGG + chr1_1:10484165-10484184 Msa0007330:intron 15.0%
!! ATTTCAATCAATTCAAACAA+AGG - chr1_1:10482824-10482843 None:intergenic 20.0%
!!! TGTTATTGATTTTCTTTGAA+TGG + chr1_1:10482858-10482877 Msa0007330:intron 20.0%
!! AAGTGATTCATTTGTTTAAA+TGG + chr1_1:10483327-10483346 Msa0007330:intron 20.0%
!!! TCTTATTTGAATTGTATTCA+TGG + chr1_1:10483824-10483843 Msa0007330:intron 20.0%
!! AATCAGAAAGAAATAATGTT+TGG - chr1_1:10483877-10483896 None:intergenic 20.0%
! AATATCTACTTAAAATCCTG+AGG - chr1_1:10482673-10482692 None:intergenic 25.0%
!! TCTCACATTTAAATGGTTTT+TGG + chr1_1:10482729-10482748 Msa0007330:intron 25.0%
!! TATTAAGACTTTTGCCATTA+AGG + chr1_1:10483266-10483285 Msa0007330:intron 25.0%
! CAATAGTTCAAATTCCTTAA+TGG - chr1_1:10483283-10483302 None:intergenic 25.0%
! AGGAATTTGAACTATTGTAA+AGG + chr1_1:10483286-10483305 Msa0007330:intron 25.0%
! TTACAAATTCAATGTAGCTA+GGG - chr1_1:10483406-10483425 None:intergenic 25.0%
! AAAGCTACAAATTCAACTTA+CGG - chr1_1:10483493-10483512 None:intergenic 25.0%
!!! TGTATGGTTTTTTTAATTGC+AGG + chr1_1:10484181-10484200 Msa0007330:intron 25.0%
CAAATTGACAAAAAACACAC+AGG - chr1_1:10482459-10482478 None:intergenic 30.0%
!!! GTTTTTTGTCAATTTGCTTC+TGG + chr1_1:10482463-10482482 Msa0007330:CDS 30.0%
AATGCAGCACAATTCAAAAT+TGG - chr1_1:10482525-10482544 None:intergenic 30.0%
TTGTGCTGCATTATTCATTT+AGG + chr1_1:10482533-10482552 Msa0007330:intron 30.0%
TGTGCTGCATTATTCATTTA+GGG + chr1_1:10482534-10482553 Msa0007330:intron 30.0%
ACATGTGCACAAACTTTAAT+TGG + chr1_1:10482588-10482607 Msa0007330:intron 30.0%
ATAACTACATCTTATGCAAG+AGG + chr1_1:10482612-10482631 Msa0007330:intron 30.0%
CTACATGTCTCACATTTAAA+TGG + chr1_1:10482722-10482741 Msa0007330:intron 30.0%
! TGATTTTCTTTGAATGGATC+AGG + chr1_1:10482864-10482883 Msa0007330:intron 30.0%
! GATTTTCTTTGAATGGATCA+GGG + chr1_1:10482865-10482884 Msa0007330:intron 30.0%
!!! CAAGATATTTTTGGTTGGAT+TGG + chr1_1:10482911-10482930 Msa0007330:CDS 30.0%
GTTCATTATGTTAGTCCTTT+AGG + chr1_1:10483349-10483368 Msa0007330:intron 30.0%
CTTACAAATTCAATGTAGCT+AGG - chr1_1:10483407-10483426 None:intergenic 30.0%
AATTCAACTTACGGTAAAGT+GGG - chr1_1:10483484-10483503 None:intergenic 30.0%
AAATTCAACTTACGGTAAAG+TGG - chr1_1:10483485-10483504 None:intergenic 30.0%
! TTTGTACCTTTTACTACTGT+TGG + chr1_1:10483535-10483554 Msa0007330:intron 30.0%
!! ATTAGTGCTGTTATTTCTCA+TGG + chr1_1:10483570-10483589 Msa0007330:intron 30.0%
AAGTAAAGCCTTGAATCATT+TGG + chr1_1:10483634-10483653 Msa0007330:intron 30.0%
ATGTACTAATGAGCTATCAA+AGG - chr1_1:10483793-10483812 None:intergenic 30.0%
AGCTCATTAGTACATATTGT+TGG + chr1_1:10483798-10483817 Msa0007330:intron 30.0%
! TTTTCTTACTATTCTACTGC+TGG + chr1_1:10484014-10484033 Msa0007330:intron 30.0%
! GGATTTTTGTTGAAGAACAT+TGG + chr1_1:10484035-10484054 Msa0007330:intron 30.0%
TCTGTTTAGAAAGCTATTTG+TGG + chr1_1:10484096-10484115 Msa0007330:intron 30.0%
ACAAAAAACACACAGGATAG+GGG - chr1_1:10482452-10482471 None:intergenic 35.0%
GACAAAAAACACACAGGATA+GGG - chr1_1:10482453-10482472 None:intergenic 35.0%
TGACAAAAAACACACAGGAT+AGG - chr1_1:10482454-10482473 None:intergenic 35.0%
!! CAATTTGCTTCTGGTTTGTA+AGG + chr1_1:10482472-10482491 Msa0007330:intron 35.0%
!! TAGCAGGTTTGTTACTTACA+AGG + chr1_1:10482495-10482514 Msa0007330:intron 35.0%
GTGCTGCATTATTCATTTAG+GGG + chr1_1:10482535-10482554 Msa0007330:intron 35.0%
CATTAAAGGTTGTTTACCTC+AGG + chr1_1:10482654-10482673 Msa0007330:intron 35.0%
!! GGAAGAGCACAAGATATTTT+TGG + chr1_1:10482902-10482921 Msa0007330:CDS 35.0%
!!! GAGCACAAGATATTTTTGGT+TGG + chr1_1:10482906-10482925 Msa0007330:CDS 35.0%
TATCAAAGAGACTGGATCTA+CGG - chr1_1:10483061-10483080 None:intergenic 35.0%
TACGAACCAACAGTAGTAAA+AGG - chr1_1:10483544-10483563 None:intergenic 35.0%
TCCATTAATGACATGTAGCA+TGG + chr1_1:10483671-10483690 Msa0007330:intron 35.0%
TCCATGCTACATGTCATTAA+TGG - chr1_1:10483675-10483694 None:intergenic 35.0%
CAAAAAACACACAGGATAGG+GGG - chr1_1:10482451-10482470 None:intergenic 40.0%
CCTTTAATGCTCAATCCAAG+TGG - chr1_1:10482643-10482662 None:intergenic 40.0%
CCACTTGGATTGAGCATTAA+AGG + chr1_1:10482640-10482659 Msa0007330:intron 40.0%
CATTCTCTCTGAGCAAACAA+TGG + chr1_1:10482699-10482718 Msa0007330:intron 40.0%
! TTTGGTTGGATTGGAGAAAG+TGG + chr1_1:10482920-10482939 Msa0007330:CDS 40.0%
! TTGGTTGGATTGGAGAAAGT+GGG + chr1_1:10482921-10482940 Msa0007330:CDS 40.0%
AGAAAGTGGGAAAAGGTGAT+TGG + chr1_1:10482934-10482953 Msa0007330:CDS 40.0%
!!! CACCGACACGGTAATTTTTT+CGG + chr1_1:10483085-10483104 Msa0007330:intron 40.0%
TGCCGAAAAAATTACCGTGT+CGG - chr1_1:10483090-10483109 None:intergenic 40.0%
TGTAGCTAGGGACTTTCATT+AGG - chr1_1:10483394-10483413 None:intergenic 40.0%
GCAGTACACCAAATGATTCA+AGG - chr1_1:10483645-10483664 None:intergenic 40.0%
GAATGAAGATCCTGCAAACT+TGG + chr1_1:10483735-10483754 Msa0007330:intron 40.0%
TCAAATCCAACCAAGTTTGC+AGG - chr1_1:10483748-10483767 None:intergenic 40.0%
!! AAGGTACGATTTGGTGCATA+AGG - chr1_1:10483774-10483793 None:intergenic 40.0%
! GAGCTATCAAAGGTACGATT+TGG - chr1_1:10483783-10483802 None:intergenic 40.0%
! CTGTTATCAAAAGTGTGCGA+AGG - chr1_1:10483853-10483872 None:intergenic 40.0%
TGACTTCAAATCTAGCTGCA+TGG + chr1_1:10483960-10483979 Msa0007330:intron 40.0%
TTCAAATCTAGCTGCATGGT+TGG + chr1_1:10483964-10483983 Msa0007330:intron 40.0%
TCAAATCTAGCTGCATGGTT+GGG + chr1_1:10483965-10483984 Msa0007330:intron 40.0%
!! CTTCTGGTTTGTAAGGTAGC+AGG + chr1_1:10482479-10482498 Msa0007330:intron 45.0%
ATGCAAGAGGAACTTCCACT+TGG + chr1_1:10482625-10482644 Msa0007330:intron 45.0%
GGATTGGAGAAAGTGGGAAA+AGG + chr1_1:10482927-10482946 Msa0007330:CDS 45.0%
GTGATTGGAGAGGAATCTCA+AGG + chr1_1:10482949-10482968 Msa0007330:CDS 45.0%
GACGGCGATTGAGATTACTT+CGG - chr1_1:10483037-10483056 None:intergenic 45.0%
GGTGGTGATATCAAAGAGAC+TGG - chr1_1:10483069-10483088 None:intergenic 45.0%
CTTTGATATCACCACCGACA+CGG + chr1_1:10483073-10483092 Msa0007330:CDS 45.0%
CGAAAAAATTACCGTGTCGG+TGG - chr1_1:10483087-10483106 None:intergenic 45.0%
GAAGATCCTGCAAACTTGGT+TGG + chr1_1:10483739-10483758 Msa0007330:intron 45.0%
GGCTGAGAAGAGACTGCTAT+TGG + chr1_1:10482556-10482575 Msa0007330:intron 50.0%
TGAATGGATCAGGGATTCCG+TGG + chr1_1:10482874-10482893 Msa0007330:intron 50.0%
TTGTGCTCTTCCTCAGTCCA+CGG - chr1_1:10482894-10482913 None:intergenic 50.0%
GTGGGAAAAGGTGATTGGAG+AGG + chr1_1:10482939-10482958 Msa0007330:CDS 50.0%
! GCATGACTTGCAACATGCGT+AGG - chr1_1:10482996-10483015 None:intergenic 50.0%
GGCGATTGAGATTACTTCGG+CGG - chr1_1:10483034-10483053 None:intergenic 50.0%
! ATCAGGGATTCCGTGGACTG+AGG + chr1_1:10482881-10482900 Msa0007330:intron 55.0%
AGAGACTGGATCTACGGCGA+CGG - chr1_1:10483055-10483074 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 10482409 10484212 10482409 ID=Msa0007330;Name=Msa0007330
chr1_1 mRNA 10482409 10484212 10482409 ID=Msa0007330-mRNA-1;Parent=Msa0007330;Name=Msa0007330-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|0.5|0.66|0.75|4|0|100
chr1_1 exon 10482409 10482484 10482409 ID=Msa0007330-mRNA-1:exon:3811;Parent=Msa0007330-mRNA-1
chr1_1 exon 10482886 10483094 10482886 ID=Msa0007330-mRNA-1:exon:3812;Parent=Msa0007330-mRNA-1
chr1_1 exon 10483363 10483370 10483363 ID=Msa0007330-mRNA-1:exon:3813;Parent=Msa0007330-mRNA-1
chr1_1 exon 10484203 10484212 10484203 ID=Msa0007330-mRNA-1:exon:3814;Parent=Msa0007330-mRNA-1
chr1_1 CDS 10482409 10482484 10482409 ID=Msa0007330-mRNA-1:cds;Parent=Msa0007330-mRNA-1
chr1_1 CDS 10482886 10483094 10482886 ID=Msa0007330-mRNA-1:cds;Parent=Msa0007330-mRNA-1
chr1_1 CDS 10483363 10483370 10483363 ID=Msa0007330-mRNA-1:cds;Parent=Msa0007330-mRNA-1
chr1_1 CDS 10484203 10484212 10484203 ID=Msa0007330-mRNA-1:cds;Parent=Msa0007330-mRNA-1
Gene Sequence

>Msa0007330

ATGTTTGTTGCAGAGTTTTTGAGAGTTGTTTTTTGTGTGCCCCCTATCCTGTGTGTTTTTTGTCAATTTGCTTCTGGGATTCCGTGGACTGAGGAAGAGCACAAGATATTTTTGGTTGGATTGGAGAAAGTGGGAAAAGGTGATTGGAGAGGAATCTCAAGGAACTATGTGAAAACAAGAACGCCTACGCATGTTGCAAGTCATGCTCAGAAGTACTTTCTCCGCCGAAGTAATCTCAATCGCCGTCGCCGTAGATCCAGTCTCTTTGATATCACCACCGACACGTCCTTTAGGATTGCATGA

Protein sequence

>Msa0007330

MFVAEFLRVVFCVPPILCVFCQFASGIPWTEEEHKIFLVGLEKVGKGDWRGISRNYVKTRTPTHVASHAQKYFLRRSNLNRRRRRSSLFDITTDTSFRIA*