AlfalfaGEDB | Msa0009410

M. sativa cultivar zhongmu-4 / Msa0009410

KEGG: -
GO: GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006884 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008361 GO:0009311 GO:0009505 GO:0009605 GO:0009607 GO:0009615 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009826 GO:0009987 GO:0009992 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0023052 GO:0030104 GO:0030312 GO:0032502 GO:0032535 GO:0032989 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055082 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0090066 GO:0098542 GO:0140096 GO:1901564 GO:1901700
PFAM: PF12947.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0009410	sp\|Q9LMN8\|WAK3_ARATH	44.323	731	310	23	491	1144	31	741	4.40e-177	541
Msa0009410	sp\|Q9LMN8\|WAK3_ARATH	37.563	394	141	13	100	392	115	504	4.22e-60	224

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0009410	A0A396JNF1	40.541	111	40	1	401	485	613	723	4.30e-13	85.9
Msa0009410	A0A396JNF1	49.806	516	137	9	1	403	1	507	4.31e-141	453
Msa0009410	A0A396JNF1	86.890	717	45	2	477	1144	24	740	0.0	1274

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0009410	MtrunA17_Chr1g0158261	86.890	717	45	2	477	1144	24	740	0.0	1274
Msa0009410	MtrunA17_Chr1g0158261	49.806	516	137	9	1	403	1	507	8.29e-145	453
Msa0009410	MtrunA17_Chr1g0158261	40.541	111	40	1	401	485	613	723	8.28e-17	85.9
Msa0009410	MtrunA17_Chr1g0158201	77.854	727	107	4	472	1144	18	744	0.0	1152
Msa0009410	MtrunA17_Chr1g0158201	50.096	521	132	13	1	403	1	511	2.78e-144	452
Msa0009410	MtrunA17_Chr1g0158201	41.584	101	33	1	401	475	617	717	1.77e-16	84.7
Msa0009410	MtrunA17_Chr1g0158251	77.428	731	107	6	472	1144	19	749	0.0	1136
Msa0009410	MtrunA17_Chr1g0158251	48.762	525	138	11	1	403	1	516	4.55e-142	446
Msa0009410	MtrunA17_Chr1g0158251	40.541	111	40	1	401	485	622	732	4.48e-17	86.7
Msa0009410	MtrunA17_Chr1g0158231	89.716	457	47	0	688	1144	4	460	0.0	858

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0009410	AT1G21240.1	44.323	731	310	23	491	1144	31	741	4.47e-178	541
Msa0009410	AT1G21240.1	37.563	394	141	13	100	392	115	504	4.29e-61	224
Msa0009410	AT1G21270.1	41.931	725	329	17	491	1144	29	732	9.89e-174	529
Msa0009410	AT1G21270.1	37.173	382	144	10	104	392	115	493	2.83e-59	218
Msa0009410	AT1G21230.1	43.094	724	323	21	491	1144	29	733	5.26e-173	527
Msa0009410	AT1G21230.1	36.591	399	149	12	92	392	105	497	1.28e-61	225
Msa0009410	AT1G21250.1	41.931	725	335	20	489	1144	28	735	4.13e-167	512
Msa0009410	AT1G21250.1	34.388	474	190	18	37	392	28	498	7.65e-59	217
Msa0009410	AT1G21210.1	39.700	733	349	18	487	1144	24	738	5.95e-163	501
Msa0009410	AT1G21210.1	34.925	398	157	9	95	392	104	499	4.37e-56	209

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Find 232 sgRNAs with CRISPR-Local

Find 714 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGATGTTTATAGCTT+TGG	0.306595	1_1:+13720826	Msa0009410:CDS
AAAATGACACAATATTACTT+TGG	0.377200	1_1:+13718520	Msa0009410:CDS
AAACATTGTCGAAACACGGA+TGG	0.542188	1_1:+13719656	Msa0009410:CDS
AAACTCAATCATTACTTCAA+GGG	0.536535	1_1:+13715313	Msa0009410:CDS
AAACTTGCAGTGTCTAAAGT+GGG	0.669802	1_1:-13718639	None:intergenic
AAAGTGCATAGCAAGACTAA+TGG	0.474114	1_1:-13720911	None:intergenic
AAATCACCTTTGCATCCATC+CGG	0.515790	1_1:-13711874	None:intergenic
AAATGACACAATATTACTTT+GGG	0.329511	1_1:+13718521	Msa0009410:CDS
AAATGTAAGGAAGGTTATGA+AGG	0.545930	1_1:+13719095	Msa0009410:CDS
AAATTACCTATGCAGCCATC+TGG	0.451604	1_1:-13719131	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCCTATTCCAAATGGATA+TGG	-	chr1_1:13711169-13711188	None:intergenic	30.0%
GATGAATTTCCTATTCCAAA+TGG	-	chr1_1:13711175-13711194	None:intergenic	30.0%
AGAAAACAAGGTCCTATATT+TGG	-	chr1_1:13711202-13711221	None:intergenic	30.0%
ATGTCTATGTCTAATACTTC+TGG	-	chr1_1:13711307-13711326	None:intergenic	30.0%
GAAAATAAGTTCATAACCGT+TGG	+	chr1_1:13711439-13711458	Msa0009410:CDS	30.0%
AACAGGAAGAAGATTCAAAA+TGG	+	chr1_1:13711541-13711560	Msa0009410:CDS	30.0%
TGCTCCAATCAAGAATTAAA+GGG	-	chr1_1:13711750-13711769	None:intergenic	30.0%
ATGCTCCAATCAAGAATTAA+AGG	-	chr1_1:13711751-13711770	None:intergenic	30.0%
ACATAAAACTCTCTCCATTT+AGG	+	chr1_1:13712054-13712073	Msa0009410:intron	30.0%
CATAAAACTCTCTCCATTTA+GGG	+	chr1_1:13712055-13712074	Msa0009410:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	13711022	13721443	13711022	ID=Msa0009410;Name=Msa0009410
chr1_1	mRNA	13711022	13721443	13711022	ID=Msa0009410-mRNA-1;Parent=Msa0009410;Name=Msa0009410-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0.16\|0.15\|1\|0.25\|0.15\|13\|159\|1144
chr1_1	exon	13711022	13711162	13711022	ID=Msa0009410-mRNA-1:exon:5216;Parent=Msa0009410-mRNA-1
chr1_1	exon	13711241	13711350	13711241	ID=Msa0009410-mRNA-1:exon:5217;Parent=Msa0009410-mRNA-1
chr1_1	exon	13711375	13711622	13711375	ID=Msa0009410-mRNA-1:exon:5218;Parent=Msa0009410-mRNA-1
chr1_1	exon	13711665	13711889	13711665	ID=Msa0009410-mRNA-1:exon:5219;Parent=Msa0009410-mRNA-1
chr1_1	exon	13714233	13714475	13714233	ID=Msa0009410-mRNA-1:exon:5220;Parent=Msa0009410-mRNA-1
chr1_1	exon	13714500	13714739	13714500	ID=Msa0009410-mRNA-1:exon:5221;Parent=Msa0009410-mRNA-1
chr1_1	exon	13715064	13715118	13715064	ID=Msa0009410-mRNA-1:exon:5222;Parent=Msa0009410-mRNA-1
chr1_1	exon	13715197	13715363	13715197	ID=Msa0009410-mRNA-1:exon:5223;Parent=Msa0009410-mRNA-1
chr1_1	exon	13718367	13718579	13718367	ID=Msa0009410-mRNA-1:exon:5224;Parent=Msa0009410-mRNA-1
chr1_1	exon	13718610	13718813	13718610	ID=Msa0009410-mRNA-1:exon:5225;Parent=Msa0009410-mRNA-1
chr1_1	exon	13718931	13719146	13718931	ID=Msa0009410-mRNA-1:exon:5226;Parent=Msa0009410-mRNA-1
chr1_1	exon	13719612	13719773	13719612	ID=Msa0009410-mRNA-1:exon:5227;Parent=Msa0009410-mRNA-1
chr1_1	exon	13720074	13721443	13720074	ID=Msa0009410-mRNA-1:exon:5228;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13711022	13711162	13711022	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13711241	13711350	13711241	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13711375	13711622	13711375	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13711665	13711889	13711665	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13714233	13714475	13714233	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13714500	13714739	13714500	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13715064	13715118	13715064	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13715197	13715363	13715197	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13718367	13718579	13718367	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13718610	13718813	13718610	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13718931	13719146	13718931	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13719612	13719773	13719612	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	CDS	13720074	13721284	13720074	ID=Msa0009410-mRNA-1:cds;Parent=Msa0009410-mRNA-1
chr1_1	three_prime_UTR	13721285	13721443	13721285	ID=Msa0009410-mRNA-1:three_prime_utr;Parent=Msa0009410-mRNA-1

Gene Sequence

>Msa0009410

ATGAAACTTAAGGCAAAACTTGCGCAATTGTGTGCTATTTTAATGTTTGCAATATTAATGATAGTACTATTGGTAGTTGAAGATGTTGAATCCCTTTCTTCAGCTCTACCTGGATGTAAAAATTCATGTGGAGATGTTGAAGTTACATGTATAGACGACTCCATATTACACTGGGGTACTAATAGTGAAAACTTAAAGAGGTCTCCAGAAGTATTAGACATAGACATCTTTAAAGGTCAAATTCAGTTATTCAGACAAAATAACAGTCGAACCTGGCTTAGATGGCCATCACACGGTTTTAGCATTTCAAGCAAGGAAAATAAGTTCATAACCGTTGGTTACAATAGTTATGGCTATCTTGATAGCAATTACGAAGGAGAAAAGTACTCAACGGGATGCATAACAAGATGTTACGGAAACAGGAAGAAGATTCAAAATGGAACGTGTTCCGGTATTGGTTGTTGTCAGATGGATATTCCAACTAACATGAGGAATATCAGATGCAGTGTTTCTTTTGTTGTCAAAAATGGATTCTACAATTTTTCTACCATTGATCTACTTGATTTCCGTTACACCAAGGTCCCTTTAATTCTTGATTGGAGCATAGAGAGCGGAAAATGCTGGAACAATAGCTTTTCTTACGACAGGGACATTGATTATGGCTACCGATGCTTATGCAAGAAAGGTTACGAAGGAAACCCATACCATCCGGATGGATGCAAAGGAGCAAGTGCGGGTTTTATTATTCTATTTGTGGGTAGTTCATCGTTGTACGTGTTATATCAAAGAAGAAAACTCTTGAAACTAAAAGAGAAATTCTTTAAACAAAATGGCGGTTTCATTTTGCTACGCCAACTTTCTAAAAGGGAAGACGCATCAGGAACTGCTCAAATTTTCAAAGAAGATGAACTTAAGAAAGCTACCAACAACTATGAGGAGAGTTTAATAATTGGCCGAGGAGGTTTCGGAGACAACAGGATTGTCGCAATCAAGAAGTCCAAAATAATGGATGCCACCCAAGTAGAGCAATTCATAAATGAGGTGGTTGTTCTGTCCCAAATAAATCATAGAAATGTTGTCAAACTCTTGGGATGTTGTTTGGAGACAGAAGTTCCTTTACTAGTCTATGAATTTATTAGCAATGGTACCCTTTTCGATTTCATACACAATAGTATAGAGAAGGCAGACAATGCAATATGGAAAATGCAAGAGAACAGAAGCCTAGCAATTCACTTCCTATCTTATTTTAGAGAGAATCGGATGTGCTTGAGACTTAAAGGGGAGGAAAGACCTAGCATGAAAGAAGTGGCTATGGAGTTGGAAGGCTTAAGGTTAATGGAGAAGCACCCTTGGACCAAATCAGAGATAAATCTTGATGAAACTCAATCATTACTTCAAGGGGCATCATCCAACTTTAGTTATGAAACTGTATTTGCATCCAATGAATCCTTTTCTTTATCCCTACCCGGTTGCAAAAACACATGTGGAGATGTCAAAATTCCATTTCCATTTGGAATATCAGATTCATCAATACCAAATCAAGGTCCCTGTTACCTAGAGCCCAAATTTGAACTCATATGTGAAAATGACACAATATTACTTTGGGGCAATGTACAAGTCTACAACATAAACATCCTCCAAGGTTATTGTAATAGCGAAAATTACTCTCAACCCACTTTAGACACTGCAAGTTTCAGGATATCAAGCACGGAAAACAAGTTCATAACGGTTGGTTGTGATAGTTACGGTTATCTCAACAGCATTTACAACGATGAAACATATTCAACCGGTTGCTTAACAAGATGTTATGGTAATCGAAATCGAATTGAAAACGGAACATGTTCGGATGGATCCTATAATTTTTCTGTTGCTCATTTGGATAATTTTCCTTATACAAAGCTTCCATTAATTCTTGATTGGAGTGTGGGAAAAACTTGTAAGGAAGATGACTATGCTTGCAAGAAGAATAGTGATTGTGTTGACGTGGCCATTGATTTTGGTTACCAATGTAAATGTAAGGAAGGTTATGAAGGAAACCCGTATCATCCAGATGGCTGCATAGACATCGACGAATGTAAGACATTGAATAACACATGCATAAGTGAAAAACATTGTCGAAACACGGATGGGTTTTATGAATGTTTTTGTCCTAATGGACAATCTGGAAATGGAGCAAAGGCAGGAGGTTGTCATAGACGAGAAGTTATTACCAAGATTGCAATTGGATCAAGTGCTGGACTAATAGTCCTATTTGTGGCGGTTTCATCTCTATACTTGACATGCCAAAAAAGGAAACTCATCAAACAAAAGCAAAAATTCTTTCAACAAAATGGTGGTTCAATTTTGATACAACAACTATCAACAAGAGAAGACACATCACAATCAGCTCAAATATTCACAGAAGAGGAACTTAAGAAAGCAACCAAAAACTACGACGAAAGCTTAATCATCGGCAAAGGAGGTTTTGGTACAGTTTTTAAAGGAATCTTGCCCGATAAAAAAATGGTCGCTGTCAAAAAGTCCAAAATAATAGACACAAATCAGATTGAGCAATTCATTAACGAGGTAGTTGTTCTGTCCCAAATAAATCACAGGAACGTCGTCAAACTTTTAGGGTGTTGTTTGGAGACAGAAGTTCCTTCATTGGTGTATGAATTTGTTAGCAACGGAACACTTTTCGATTTCATACAAAATTGTAAAGATAAAAAAAATAATCCAACATGGAAAACACGTTTAAGGATTGCAGCAGAGACAGCTGGAGCTTTATCATATCTACATTCAGCTGCATCAATTCCAATTATCCATAGAGATGTTAAGAGTACCAACATTCTCTTGGATGACAATTACACTGCAAAAGTTTCCGACTTCGGAGCTTCAAGATTGGTTCCACTTGATCAAACCGAGATAGCAACTATGGTACAAGGAACTCTTGGATACTTAGATCCAGAGTATATGCAAACACATCAATTAACCGATAAAAGTGATGTTTATAGCTTTGGAGTGGTTCTTGCAGAGCTTCTAACAGGTGACAAACCTCTTTCTTTTAACAGGCCAGAAGAGACCATTAGTCTTGCTATGCACTTTTTGTCTTGTTTAAAACAAGATAAACTTTTTGAAGCTATTCAAGTTGGAATTTGGGATGATGATAACAAGAAAGAGATTAAGGAGGTTTCTATTCTTGCTGCAAGGTGTTTAAGGCTTAGAGGTGATGAAAGACCTAGTATGAGGGAAGTAGCTATGGAGTTGGATGGCATAAGGTTAATGGAGAAGCATCCTTGGAATGATACAGAACAAAATTTTGAGGAGAGCCAACGCTTACTTCATGAGGCATCTTGTAGTATCTACAATGAAACTGGTGATAGCTATAACCCTGGATATAGTGGGTATGATAGCTTGAAAGATCAACCATTGATTGTCTTGGATGATGGAAGATGA

Protein sequence

>Msa0009410

MKLKAKLAQLCAILMFAILMIVLLVVEDVESLSSALPGCKNSCGDVEVTCIDDSILHWGTNSENLKRSPEVLDIDIFKGQIQLFRQNNSRTWLRWPSHGFSISSKENKFITVGYNSYGYLDSNYEGEKYSTGCITRCYGNRKKIQNGTCSGIGCCQMDIPTNMRNIRCSVSFVVKNGFYNFSTIDLLDFRYTKVPLILDWSIESGKCWNNSFSYDRDIDYGYRCLCKKGYEGNPYHPDGCKGASAGFIILFVGSSSLYVLYQRRKLLKLKEKFFKQNGGFILLRQLSKREDASGTAQIFKEDELKKATNNYEESLIIGRGGFGDNRIVAIKKSKIMDATQVEQFINEVVVLSQINHRNVVKLLGCCLETEVPLLVYEFISNGTLFDFIHNSIEKADNAIWKMQENRSLAIHFLSYFRENRMCLRLKGEERPSMKEVAMELEGLRLMEKHPWTKSEINLDETQSLLQGASSNFSYETVFASNESFSLSLPGCKNTCGDVKIPFPFGISDSSIPNQGPCYLEPKFELICENDTILLWGNVQVYNINILQGYCNSENYSQPTLDTASFRISSTENKFITVGCDSYGYLNSIYNDETYSTGCLTRCYGNRNRIENGTCSDGSYNFSVAHLDNFPYTKLPLILDWSVGKTCKEDDYACKKNSDCVDVAIDFGYQCKCKEGYEGNPYHPDGCIDIDECKTLNNTCISEKHCRNTDGFYECFCPNGQSGNGAKAGGCHRREVITKIAIGSSAGLIVLFVAVSSLYLTCQKRKLIKQKQKFFQQNGGSILIQQLSTREDTSQSAQIFTEEELKKATKNYDESLIIGKGGFGTVFKGILPDKKMVAVKKSKIIDTNQIEQFINEVVVLSQINHRNVVKLLGCCLETEVPSLVYEFVSNGTLFDFIQNCKDKKNNPTWKTRLRIAAETAGALSYLHSAASIPIIHRDVKSTNILLDDNYTAKVSDFGASRLVPLDQTEIATMVQGTLGYLDPEYMQTHQLTDKSDVYSFGVVLAELLTGDKPLSFNRPEETISLAMHFLSCLKQDKLFEAIQVGIWDDDNKKEIKEVSILAARCLRLRGDERPSMREVAMELDGIRLMEKHPWNDTEQNFEESQRLLHEASCSIYNETGDSYNPGYSGYDSLKDQPLIVLDDGR*