Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010180 | AFK49669.1 | 92.222 | 90 | 7 | 0 | 32 | 121 | 1 | 90 | 1.31e-54 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010180 | sp|Q9SLG0|NFYB1_ARATH | 40.777 | 103 | 58 | 1 | 38 | 137 | 26 | 128 | 6.38e-21 | 85.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0010180 | TF | NF-YB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010180 | MtrunA17_Chr1g0159291 | 92.222 | 90 | 7 | 0 | 32 | 121 | 1 | 90 | 1.20e-58 | 176 |
| Msa0010180 | MtrunA17_Chr3g0102351 | 39.394 | 99 | 59 | 1 | 38 | 136 | 32 | 129 | 4.06e-21 | 84.3 |
| Msa0010180 | MtrunA17_Chr1g0195851 | 34.783 | 115 | 75 | 0 | 7 | 121 | 10 | 124 | 1.07e-20 | 83.2 |
| Msa0010180 | MtrunA17_Chr1g0185911 | 39.394 | 99 | 52 | 1 | 38 | 128 | 34 | 132 | 1.07e-20 | 82.8 |
| Msa0010180 | MtrunA17_Chr8g0382931 | 39.604 | 101 | 60 | 1 | 38 | 138 | 33 | 132 | 1.42e-20 | 83.2 |
| Msa0010180 | MtrunA17_Chr5g0446491 | 42.857 | 84 | 48 | 0 | 38 | 121 | 28 | 111 | 2.01e-20 | 82.4 |
| Msa0010180 | MtrunA17_Chr4g0076381 | 39.286 | 84 | 51 | 0 | 38 | 121 | 63 | 146 | 2.05e-20 | 83.6 |
| Msa0010180 | MtrunA17_Chr7g0263601 | 37.383 | 107 | 63 | 2 | 38 | 141 | 31 | 136 | 3.40e-20 | 81.6 |
| Msa0010180 | MtrunA17_Chr2g0290491 | 41.573 | 89 | 51 | 1 | 38 | 126 | 22 | 109 | 3.53e-20 | 81.3 |
| Msa0010180 | MtrunA17_Chr4g0076321 | 40.909 | 88 | 52 | 0 | 34 | 121 | 20 | 107 | 5.37e-20 | 80.1 |
| Msa0010180 | MtrunA17_Chr1g0159271 | 45.161 | 93 | 50 | 1 | 47 | 138 | 1 | 93 | 9.52e-20 | 78.6 |
| Msa0010180 | MtrunA17_Chr1g0165041 | 41.667 | 84 | 49 | 0 | 38 | 121 | 11 | 94 | 1.13e-19 | 80.5 |
| Msa0010180 | MtrunA17_Chr8g0384451 | 41.176 | 85 | 50 | 0 | 38 | 122 | 45 | 129 | 5.88e-19 | 79.3 |
| Msa0010180 | MtrunA17_Chr1g0191981 | 35.714 | 84 | 54 | 0 | 38 | 121 | 9 | 92 | 1.53e-18 | 75.9 |
| Msa0010180 | MtrunA17_Chr2g0296321 | 38.095 | 84 | 52 | 0 | 38 | 121 | 71 | 154 | 9.48e-18 | 76.6 |
| Msa0010180 | MtrunA17_Chr4g0067091 | 38.095 | 84 | 52 | 0 | 38 | 121 | 27 | 110 | 1.64e-17 | 75.5 |
| Msa0010180 | MtrunA17_Chr1g0158951 | 37.778 | 90 | 42 | 1 | 63 | 138 | 19 | 108 | 3.97e-16 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010180 | AT2G38880.7 | 41.584 | 101 | 58 | 1 | 38 | 137 | 26 | 126 | 2.89e-22 | 86.3 |
| Msa0010180 | AT2G38880.3 | 40.777 | 103 | 58 | 1 | 38 | 137 | 26 | 128 | 5.56e-22 | 85.5 |
| Msa0010180 | AT2G38880.2 | 40.777 | 103 | 58 | 1 | 38 | 137 | 26 | 128 | 6.49e-22 | 85.5 |
| Msa0010180 | AT2G38880.1 | 40.777 | 103 | 58 | 1 | 38 | 137 | 26 | 128 | 6.49e-22 | 85.5 |
| Msa0010180 | AT2G38880.5 | 40.777 | 103 | 58 | 1 | 38 | 137 | 26 | 128 | 6.49e-22 | 85.5 |
| Msa0010180 | AT3G53340.5 | 41.346 | 104 | 58 | 1 | 38 | 138 | 34 | 137 | 2.56e-21 | 84.7 |
| Msa0010180 | AT3G53340.1 | 41.346 | 104 | 58 | 1 | 38 | 138 | 34 | 137 | 2.56e-21 | 84.7 |
| Msa0010180 | AT2G37060.2 | 40.385 | 104 | 59 | 1 | 38 | 138 | 35 | 138 | 1.15e-20 | 83.2 |
| Msa0010180 | AT2G37060.3 | 40.385 | 104 | 59 | 1 | 38 | 138 | 35 | 138 | 1.15e-20 | 83.2 |
| Msa0010180 | AT2G37060.1 | 40.385 | 104 | 59 | 1 | 38 | 138 | 35 | 138 | 1.15e-20 | 83.2 |
| Msa0010180 | AT2G38880.4 | 42.353 | 85 | 49 | 0 | 38 | 122 | 26 | 110 | 1.15e-20 | 81.3 |
| Msa0010180 | AT4G14540.1 | 42.857 | 84 | 48 | 0 | 38 | 121 | 26 | 109 | 1.32e-20 | 82.8 |
| Msa0010180 | AT5G47640.1 | 43.529 | 85 | 48 | 0 | 38 | 122 | 32 | 116 | 2.93e-20 | 82.4 |
| Msa0010180 | AT2G38880.11 | 42.857 | 84 | 48 | 0 | 38 | 121 | 26 | 109 | 3.96e-20 | 81.6 |
| Msa0010180 | AT2G38880.8 | 42.857 | 84 | 48 | 0 | 38 | 121 | 26 | 109 | 3.96e-20 | 81.6 |
| Msa0010180 | AT2G38880.9 | 42.857 | 84 | 48 | 0 | 38 | 121 | 26 | 109 | 7.87e-20 | 80.5 |
| Msa0010180 | AT2G38880.10 | 42.857 | 84 | 48 | 0 | 38 | 121 | 26 | 109 | 7.87e-20 | 80.5 |
| Msa0010180 | AT2G38880.6 | 42.857 | 84 | 48 | 0 | 38 | 121 | 26 | 109 | 7.87e-20 | 80.5 |
| Msa0010180 | AT2G47810.1 | 40.476 | 84 | 50 | 0 | 38 | 121 | 56 | 139 | 2.54e-19 | 79.3 |
| Msa0010180 | AT3G53340.4 | 44.048 | 84 | 47 | 0 | 38 | 121 | 34 | 117 | 2.95e-19 | 78.2 |
| Msa0010180 | AT5G47670.2 | 35.789 | 95 | 61 | 0 | 38 | 132 | 34 | 128 | 1.05e-18 | 78.6 |
| Msa0010180 | AT1G21970.1 | 34.426 | 122 | 74 | 2 | 2 | 121 | 30 | 147 | 1.59e-18 | 79.0 |
| Msa0010180 | AT5G47670.1 | 35.789 | 95 | 61 | 0 | 38 | 132 | 63 | 157 | 2.23e-18 | 78.6 |
| Msa0010180 | AT5G47670.3 | 35.789 | 95 | 61 | 0 | 38 | 132 | 63 | 157 | 2.23e-18 | 78.6 |
| Msa0010180 | AT3G53340.3 | 44.048 | 84 | 47 | 0 | 38 | 121 | 34 | 117 | 3.81e-18 | 75.9 |
| Msa0010180 | AT3G53340.2 | 44.048 | 84 | 47 | 0 | 38 | 121 | 34 | 117 | 3.81e-18 | 75.9 |
| Msa0010180 | AT2G13570.1 | 41.176 | 85 | 50 | 0 | 38 | 122 | 41 | 125 | 9.05e-18 | 76.6 |
| Msa0010180 | AT1G09030.1 | 32.673 | 101 | 61 | 1 | 38 | 131 | 8 | 108 | 1.88e-17 | 73.9 |
Find 32 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGTTCTACATTGGCTTTC+TGG | 0.102414 | 1_1:-15243280 | None:intergenic |
| ATTGTGTGTGTCTAAATTTA+TGG | 0.291429 | 1_1:+15243235 | Msa0010180:CDS |
| TTTCTGCTGATGATTTACTA+TGG | 0.320933 | 1_1:+15243312 | Msa0010180:CDS |
| CGCACTTCCTCGCCCATTGT+TGG | 0.335463 | 1_1:-15243125 | None:intergenic |
| GAGGAATTTGTTAGGTCTCT+TGG | 0.362755 | 1_1:+15243356 | Msa0010180:CDS |
| ATTTACTATGGACGATGAAT+AGG | 0.363355 | 1_1:+15243324 | Msa0010180:CDS |
| TGGGCTTTGAGGAATTTGTT+AGG | 0.401864 | 1_1:+15243348 | Msa0010180:CDS |
| AGGAATTTGTTAGGTCTCTT+GGG | 0.404606 | 1_1:+15243357 | Msa0010180:CDS |
| GGAGTTGAATGTTGGTTCTC+AGG | 0.413844 | 1_1:-15243104 | None:intergenic |
| GATGAATAGGTTGGGCTTTG+AGG | 0.427694 | 1_1:+15243337 | Msa0010180:CDS |
| CTATGGACGATGAATAGGTT+GGG | 0.452990 | 1_1:+15243329 | Msa0010180:CDS |
| GACTCTTGTCACATGATTGA+TGG | 0.464195 | 1_1:-15243153 | None:intergenic |
| CGCAACCACATGGATGGGTC+AGG | 0.482696 | 1_1:+15243398 | Msa0010180:CDS |
| CCAACATTCAACTCCAACAA+TGG | 0.484164 | 1_1:+15243112 | Msa0010180:CDS |
| TTAACGTAGGTCATCATCAT+TGG | 0.489301 | 1_1:-15243441 | None:intergenic |
| AACTATCGCAACCACATGGA+TGG | 0.490022 | 1_1:+15243392 | Msa0010180:CDS |
| CAACATTCAACTCCAACAAT+GGG | 0.502283 | 1_1:+15243113 | Msa0010180:CDS |
| CCATTGTTGGAGTTGAATGT+TGG | 0.510977 | 1_1:-15243112 | None:intergenic |
| CATTAACTCCTTAGCATCAC+CGG | 0.525471 | 1_1:-15243210 | None:intergenic |
| ACTATGGACGATGAATAGGT+TGG | 0.532041 | 1_1:+15243328 | Msa0010180:CDS |
| ATGGATGGGTCAGGTGCTAG+TGG | 0.541112 | 1_1:+15243407 | Msa0010180:CDS |
| TCACCGGTGATTATTGTGTC+CGG | 0.543765 | 1_1:-15243194 | None:intergenic |
| AATCATTTGATGTTCTACAT+TGG | 0.545949 | 1_1:-15243288 | None:intergenic |
| AATAATCACCGGTGATGCTA+AGG | 0.556556 | 1_1:+15243202 | Msa0010180:CDS |
| CATGCAGAGTGTTCTTCCAC+CGG | 0.588890 | 1_1:+15243175 | Msa0010180:CDS |
| AACAACAACAACATTGACGT+TGG | 0.590713 | 1_1:+15243080 | Msa0010180:CDS |
| ACTCTTGTCACATGATTGAT+GGG | 0.597608 | 1_1:-15243152 | None:intergenic |
| TTCAACTCCAACAATGGGCG+AGG | 0.597648 | 1_1:+15243118 | Msa0010180:CDS |
| ACTATCGCAACCACATGGAT+GGG | 0.604393 | 1_1:+15243393 | Msa0010180:CDS |
| CCGGTGATTATTGTGTCCGG+TGG | 0.615417 | 1_1:-15243191 | None:intergenic |
| CCACCGGACACAATAATCAC+CGG | 0.624454 | 1_1:+15243191 | Msa0010180:CDS |
| TAGCACCTGACCCATCCATG+TGG | 0.736156 | 1_1:-15243403 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGTGTGTGTCTAAATTTA+TGG | + | chr1_1:15243235-15243254 | Msa0010180:CDS | 25.0% |
| ! | AATCATTTGATGTTCTACAT+TGG | - | chr1_1:15243291-15243310 | None:intergenic | 25.0% |
| !! | TTAGCTACAATGTTTTTGAC+AGG | - | chr1_1:15243044-15243063 | None:intergenic | 30.0% |
| TTTCTGCTGATGATTTACTA+TGG | + | chr1_1:15243312-15243331 | Msa0010180:CDS | 30.0% | |
| ATTTACTATGGACGATGAAT+AGG | + | chr1_1:15243324-15243343 | Msa0010180:CDS | 30.0% | |
| ! | AATGTTGTTGTTGTTGTTGG+TGG | - | chr1_1:15243075-15243094 | None:intergenic | 35.0% |
| ! | GTCAATGTTGTTGTTGTTGT+TGG | - | chr1_1:15243078-15243097 | None:intergenic | 35.0% |
| AACAACAACAACATTGACGT+TGG | + | chr1_1:15243080-15243099 | Msa0010180:CDS | 35.0% | |
| CAACATTCAACTCCAACAAT+GGG | + | chr1_1:15243113-15243132 | Msa0010180:CDS | 35.0% | |
| ACTCTTGTCACATGATTGAT+GGG | - | chr1_1:15243155-15243174 | None:intergenic | 35.0% | |
| !! | AGGAATTTGTTAGGTCTCTT+GGG | + | chr1_1:15243357-15243376 | Msa0010180:CDS | 35.0% |
| TTAACGTAGGTCATCATCAT+TGG | - | chr1_1:15243444-15243463 | None:intergenic | 35.0% | |
| CCATTGTTGGAGTTGAATGT+TGG | - | chr1_1:15243115-15243134 | None:intergenic | 40.0% | |
| CCAACATTCAACTCCAACAA+TGG | + | chr1_1:15243112-15243131 | Msa0010180:CDS | 40.0% | |
| GACTCTTGTCACATGATTGA+TGG | - | chr1_1:15243156-15243175 | None:intergenic | 40.0% | |
| AATAATCACCGGTGATGCTA+AGG | + | chr1_1:15243202-15243221 | Msa0010180:CDS | 40.0% | |
| CATTAACTCCTTAGCATCAC+CGG | - | chr1_1:15243213-15243232 | None:intergenic | 40.0% | |
| !! | GATGTTCTACATTGGCTTTC+TGG | - | chr1_1:15243283-15243302 | None:intergenic | 40.0% |
| ACTATGGACGATGAATAGGT+TGG | + | chr1_1:15243328-15243347 | Msa0010180:CDS | 40.0% | |
| CTATGGACGATGAATAGGTT+GGG | + | chr1_1:15243329-15243348 | Msa0010180:CDS | 40.0% | |
| !! | TGGGCTTTGAGGAATTTGTT+AGG | + | chr1_1:15243348-15243367 | Msa0010180:CDS | 40.0% |
| !! | GAGGAATTTGTTAGGTCTCT+TGG | + | chr1_1:15243356-15243375 | Msa0010180:CDS | 40.0% |
| ! | TGTGGTTGCGATAGTTTTTG+AGG | - | chr1_1:15243388-15243407 | None:intergenic | 40.0% |
| CAAAAACTATCGCAACCACA+TGG | + | chr1_1:15243388-15243407 | Msa0010180:CDS | 40.0% | |
| !! | GGAGTTGAATGTTGGTTCTC+AGG | - | chr1_1:15243107-15243126 | None:intergenic | 45.0% |
| TCACCGGTGATTATTGTGTC+CGG | - | chr1_1:15243197-15243216 | None:intergenic | 45.0% | |
| ! | GATGAATAGGTTGGGCTTTG+AGG | + | chr1_1:15243337-15243356 | Msa0010180:CDS | 45.0% |
| AACTATCGCAACCACATGGA+TGG | + | chr1_1:15243392-15243411 | Msa0010180:CDS | 45.0% | |
| ACTATCGCAACCACATGGAT+GGG | + | chr1_1:15243393-15243412 | Msa0010180:CDS | 45.0% | |
| TTCAACTCCAACAATGGGCG+AGG | + | chr1_1:15243118-15243137 | Msa0010180:CDS | 50.0% | |
| ! | CATGCAGAGTGTTCTTCCAC+CGG | + | chr1_1:15243175-15243194 | Msa0010180:CDS | 50.0% |
| CCACCGGACACAATAATCAC+CGG | + | chr1_1:15243191-15243210 | Msa0010180:CDS | 50.0% | |
| CCGGTGATTATTGTGTCCGG+TGG | - | chr1_1:15243194-15243213 | None:intergenic | 55.0% | |
| TAGCACCTGACCCATCCATG+TGG | - | chr1_1:15243406-15243425 | None:intergenic | 55.0% | |
| ATGGATGGGTCAGGTGCTAG+TGG | + | chr1_1:15243407-15243426 | Msa0010180:CDS | 55.0% | |
| CGCACTTCCTCGCCCATTGT+TGG | - | chr1_1:15243128-15243147 | None:intergenic | 60.0% | |
| CGCAACCACATGGATGGGTC+AGG | + | chr1_1:15243398-15243417 | Msa0010180:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 15243038 | 15243463 | 15243038 | ID=Msa0010180;Name=Msa0010180 |
| chr1_1 | mRNA | 15243038 | 15243463 | 15243038 | ID=Msa0010180-mRNA-1;Parent=Msa0010180;Name=Msa0010180-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|141 |
| chr1_1 | exon | 15243038 | 15243463 | 15243038 | ID=Msa0010180-mRNA-1:exon:5609;Parent=Msa0010180-mRNA-1 |
| chr1_1 | CDS | 15243038 | 15243463 | 15243038 | ID=Msa0010180-mRNA-1:cds;Parent=Msa0010180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
