Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010190 | sp|Q9SLG0|NFYB1_ARATH | 39.806 | 103 | 59 | 1 | 34 | 133 | 26 | 128 | 5.83e-20 | 82.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0010190 | TF | NF-YB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010190 | MtrunA17_Chr1g0159291 | 81.111 | 90 | 17 | 0 | 28 | 117 | 1 | 90 | 1.83e-48 | 150 |
| Msa0010190 | MtrunA17_Chr4g0076381 | 37.383 | 107 | 64 | 1 | 12 | 118 | 44 | 147 | 3.62e-21 | 85.5 |
| Msa0010190 | MtrunA17_Chr1g0165041 | 38.393 | 112 | 58 | 2 | 15 | 126 | 2 | 102 | 2.12e-20 | 82.4 |
| Msa0010190 | MtrunA17_Chr1g0185911 | 39.394 | 99 | 52 | 1 | 34 | 124 | 34 | 132 | 2.18e-20 | 82.0 |
| Msa0010190 | MtrunA17_Chr3g0102351 | 39.175 | 97 | 58 | 1 | 34 | 130 | 32 | 127 | 1.04e-19 | 80.5 |
| Msa0010190 | MtrunA17_Chr7g0263601 | 36.449 | 107 | 65 | 1 | 34 | 137 | 31 | 137 | 2.93e-19 | 79.0 |
| Msa0010190 | MtrunA17_Chr1g0195851 | 40.000 | 85 | 51 | 0 | 34 | 118 | 41 | 125 | 3.76e-19 | 79.3 |
| Msa0010190 | MtrunA17_Chr8g0382931 | 38.614 | 101 | 61 | 1 | 34 | 134 | 33 | 132 | 4.49e-19 | 79.3 |
| Msa0010190 | MtrunA17_Chr5g0446491 | 41.176 | 85 | 50 | 0 | 34 | 118 | 28 | 112 | 5.27e-19 | 78.6 |
| Msa0010190 | MtrunA17_Chr2g0290491 | 40.449 | 89 | 52 | 1 | 34 | 122 | 22 | 109 | 6.72e-19 | 77.8 |
| Msa0010190 | MtrunA17_Chr4g0076321 | 39.773 | 88 | 53 | 0 | 30 | 117 | 20 | 107 | 1.15e-18 | 76.6 |
| Msa0010190 | MtrunA17_Chr8g0384451 | 40.476 | 84 | 50 | 0 | 34 | 117 | 45 | 128 | 1.25e-17 | 75.5 |
| Msa0010190 | MtrunA17_Chr1g0191981 | 34.118 | 85 | 56 | 0 | 34 | 118 | 9 | 93 | 3.46e-17 | 72.4 |
| Msa0010190 | MtrunA17_Chr2g0296321 | 32.727 | 110 | 64 | 1 | 9 | 118 | 56 | 155 | 3.76e-17 | 75.1 |
| Msa0010190 | MtrunA17_Chr4g0067091 | 36.471 | 85 | 54 | 0 | 34 | 118 | 27 | 111 | 4.21e-16 | 71.6 |
| Msa0010190 | MtrunA17_Chr1g0158951 | 39.535 | 86 | 38 | 1 | 65 | 136 | 25 | 110 | 7.99e-16 | 68.6 |
| Msa0010190 | MtrunA17_Chr1g0159271 | 41.333 | 75 | 44 | 0 | 43 | 117 | 1 | 75 | 2.24e-14 | 64.7 |
| Msa0010190 | MtrunA17_Chr5g0446601 | 30.952 | 84 | 58 | 0 | 34 | 117 | 29 | 112 | 2.94e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0010190 | AT2G38880.7 | 40.594 | 101 | 59 | 1 | 34 | 133 | 26 | 126 | 3.05e-21 | 83.6 |
| Msa0010190 | AT2G38880.3 | 39.806 | 103 | 59 | 1 | 34 | 133 | 26 | 128 | 5.30e-21 | 82.8 |
| Msa0010190 | AT2G38880.2 | 39.806 | 103 | 59 | 1 | 34 | 133 | 26 | 128 | 5.93e-21 | 82.8 |
| Msa0010190 | AT2G38880.1 | 39.806 | 103 | 59 | 1 | 34 | 133 | 26 | 128 | 5.93e-21 | 82.8 |
| Msa0010190 | AT2G38880.5 | 39.806 | 103 | 59 | 1 | 34 | 133 | 26 | 128 | 5.93e-21 | 82.8 |
| Msa0010190 | AT3G53340.5 | 40.566 | 106 | 60 | 1 | 34 | 136 | 34 | 139 | 6.87e-21 | 83.6 |
| Msa0010190 | AT3G53340.1 | 40.566 | 106 | 60 | 1 | 34 | 136 | 34 | 139 | 6.87e-21 | 83.6 |
| Msa0010190 | AT2G37060.2 | 39.623 | 106 | 61 | 1 | 34 | 136 | 35 | 140 | 1.65e-20 | 82.4 |
| Msa0010190 | AT2G37060.3 | 39.623 | 106 | 61 | 1 | 34 | 136 | 35 | 140 | 1.65e-20 | 82.4 |
| Msa0010190 | AT2G37060.1 | 39.623 | 106 | 61 | 1 | 34 | 136 | 35 | 140 | 1.65e-20 | 82.4 |
| Msa0010190 | AT2G38880.4 | 41.176 | 85 | 50 | 0 | 34 | 118 | 26 | 110 | 1.31e-19 | 78.6 |
| Msa0010190 | AT4G14540.1 | 36.111 | 108 | 68 | 1 | 11 | 118 | 4 | 110 | 2.63e-19 | 79.0 |
| Msa0010190 | AT2G38880.11 | 41.176 | 85 | 50 | 0 | 34 | 118 | 26 | 110 | 3.65e-19 | 79.0 |
| Msa0010190 | AT2G38880.8 | 41.176 | 85 | 50 | 0 | 34 | 118 | 26 | 110 | 3.65e-19 | 79.0 |
| Msa0010190 | AT1G21970.1 | 35.514 | 107 | 69 | 0 | 12 | 118 | 42 | 148 | 3.86e-19 | 80.5 |
| Msa0010190 | AT5G47640.1 | 42.353 | 85 | 49 | 0 | 34 | 118 | 32 | 116 | 4.12e-19 | 79.3 |
| Msa0010190 | AT2G38880.9 | 41.176 | 85 | 50 | 0 | 34 | 118 | 26 | 110 | 5.63e-19 | 78.2 |
| Msa0010190 | AT2G38880.10 | 41.176 | 85 | 50 | 0 | 34 | 118 | 26 | 110 | 5.63e-19 | 78.2 |
| Msa0010190 | AT2G38880.6 | 41.176 | 85 | 50 | 0 | 34 | 118 | 26 | 110 | 5.63e-19 | 78.2 |
| Msa0010190 | AT5G47670.2 | 36.842 | 95 | 60 | 0 | 34 | 128 | 34 | 128 | 6.79e-19 | 79.0 |
| Msa0010190 | AT5G47670.1 | 36.842 | 95 | 60 | 0 | 34 | 128 | 63 | 157 | 1.25e-18 | 79.0 |
| Msa0010190 | AT5G47670.3 | 36.842 | 95 | 60 | 0 | 34 | 128 | 63 | 157 | 1.25e-18 | 79.0 |
| Msa0010190 | AT3G53340.4 | 42.353 | 85 | 49 | 0 | 34 | 118 | 34 | 118 | 2.50e-18 | 75.5 |
| Msa0010190 | AT2G47810.1 | 35.577 | 104 | 64 | 1 | 17 | 117 | 36 | 139 | 1.49e-17 | 74.7 |
| Msa0010190 | AT3G53340.3 | 41.053 | 95 | 55 | 1 | 34 | 128 | 34 | 127 | 1.56e-17 | 74.3 |
| Msa0010190 | AT3G53340.2 | 41.053 | 95 | 55 | 1 | 34 | 128 | 34 | 127 | 1.56e-17 | 74.3 |
| Msa0010190 | AT2G13570.1 | 40.476 | 84 | 50 | 0 | 34 | 117 | 41 | 124 | 1.97e-16 | 72.8 |
| Msa0010190 | AT1G09030.1 | 30.337 | 89 | 62 | 0 | 30 | 118 | 4 | 92 | 3.62e-14 | 65.1 |
Find 35 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGTTTGTGTCTAAATTTA+AGG | 0.219243 | 1_1:+15246819 | Msa0010190:CDS |
| CGCATTTCCTCGCCCATTGC+TGG | 0.275956 | 1_1:-15246709 | None:intergenic |
| TTTCCGCTGATGATTTACTA+TGG | 0.310491 | 1_1:+15246896 | Msa0010190:CDS |
| GAGGAACATGTTCGGTCTCT+TGG | 0.363705 | 1_1:+15246940 | Msa0010190:CDS |
| ATTTACTATGGGCGATGAAT+AGG | 0.367938 | 1_1:+15246908 | Msa0010190:CDS |
| GATGTTCTACATTGGCTTGT+TGG | 0.413393 | 1_1:-15246864 | None:intergenic |
| GATGAATAGGTTGGGCTTTG+AGG | 0.444184 | 1_1:+15246921 | Msa0010190:CDS |
| AGGAACATGTTCGGTCTCTT+GGG | 0.445239 | 1_1:+15246941 | Msa0010190:CDS |
| ACCAACAACAACACTGGCAT+TGG | 0.455824 | 1_1:+15246664 | Msa0010190:CDS |
| CTATGGGCGATGAATAGGTT+GGG | 0.459758 | 1_1:+15246913 | Msa0010190:CDS |
| TCCAATGCCAGTGTTGTTGT+TGG | 0.470529 | 1_1:-15246665 | None:intergenic |
| TCAACGTTGGTCATCATCAT+TGG | 0.472149 | 1_1:-15247025 | None:intergenic |
| CGCAAGAACATGGATGGGTC+AGG | 0.479316 | 1_1:+15246982 | Msa0010190:CDS |
| AATGCCAGTGTTGTTGTTGG+TGG | 0.486016 | 1_1:-15246662 | None:intergenic |
| TCATCAGTGATTGTTGTGTC+TGG | 0.489745 | 1_1:-15246778 | None:intergenic |
| CAACATTCAACTCCAGCAAT+GGG | 0.494824 | 1_1:+15246697 | Msa0010190:CDS |
| GACTCTTGTCACATGACTGA+TGG | 0.498145 | 1_1:-15246737 | None:intergenic |
| AACTATCGCAAGAACATGGA+TGG | 0.501574 | 1_1:+15246976 | Msa0010190:CDS |
| TGGGCTTTGAGGAACATGTT+CGG | 0.504221 | 1_1:+15246932 | Msa0010190:CDS |
| TTCCGCTGATGATTTACTAT+GGG | 0.529918 | 1_1:+15246897 | Msa0010190:CDS |
| CCATTGCTGGAGTTGAATGT+TGG | 0.538932 | 1_1:-15246696 | None:intergenic |
| CCAACATTCAACTCCAGCAA+TGG | 0.540089 | 1_1:+15246696 | Msa0010190:CDS |
| AACAATCACTGATGATGCTA+AGG | 0.542129 | 1_1:+15246786 | Msa0010190:CDS |
| ATTCTCTTGATGTTCTACAT+TGG | 0.543777 | 1_1:-15246872 | None:intergenic |
| ACTATGGGCGATGAATAGGT+TGG | 0.546503 | 1_1:+15246912 | Msa0010190:CDS |
| AATTAATTTCATATCAACGT+TGG | 0.558973 | 1_1:-15247038 | None:intergenic |
| TTCAACTCCAGCAATGGGCG+AGG | 0.561544 | 1_1:+15246702 | Msa0010190:CDS |
| ATGGATGGGTCAGGTGCTAG+TGG | 0.576300 | 1_1:+15246991 | Msa0010190:CDS |
| TGTGTCTAAATTTAAGGACA+AGG | 0.583205 | 1_1:+15246825 | Msa0010190:CDS |
| CAACACTGGCATTGGAACTG+AGG | 0.595180 | 1_1:+15246672 | Msa0010190:CDS |
| ACAACCACCAACAACAACAC+TGG | 0.602295 | 1_1:+15246658 | Msa0010190:CDS |
| TCAGTGATTGTTGTGTCTGG+TGG | 0.604688 | 1_1:-15246775 | None:intergenic |
| TAAGGACAAGGTCACAAGTG+AGG | 0.624680 | 1_1:+15246837 | Msa0010190:CDS |
| ACTATCGCAAGAACATGGAT+GGG | 0.635414 | 1_1:+15246977 | Msa0010190:CDS |
| CGCCCATAGTAAATCATCAG+CGG | 0.639519 | 1_1:-15246899 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTCTTCTTTTTAATCTA+AGG | - | chr1_1:15246544-15246563 | None:intergenic | 20.0% |
| !!! | ATGTTTTTAATAGAGGTAAA+AGG | - | chr1_1:15246622-15246641 | None:intergenic | 20.0% |
| !! | ATTGTTTGTGTCTAAATTTA+AGG | + | chr1_1:15246819-15246838 | Msa0010190:CDS | 20.0% |
| !! | TTCTTCTTTTTAATCTAAGG+TGG | - | chr1_1:15246541-15246560 | None:intergenic | 25.0% |
| !!! | AGCTAAGATGTTTTTAATAG+AGG | - | chr1_1:15246629-15246648 | None:intergenic | 25.0% |
| ! | TGTAGTCACATTTTACAAGT+TGG | + | chr1_1:15246511-15246530 | Msa0010190:intron | 30.0% |
| TGTGTCTAAATTTAAGGACA+AGG | + | chr1_1:15246825-15246844 | Msa0010190:CDS | 30.0% | |
| ATTCTCTTGATGTTCTACAT+TGG | - | chr1_1:15246875-15246894 | None:intergenic | 30.0% | |
| ! | AACAATCACTGATGATGCTA+AGG | + | chr1_1:15246786-15246805 | Msa0010190:CDS | 35.0% |
| TTTCCGCTGATGATTTACTA+TGG | + | chr1_1:15246896-15246915 | Msa0010190:CDS | 35.0% | |
| TTCCGCTGATGATTTACTAT+GGG | + | chr1_1:15246897-15246916 | Msa0010190:CDS | 35.0% | |
| ATTTACTATGGGCGATGAAT+AGG | + | chr1_1:15246908-15246927 | Msa0010190:CDS | 35.0% | |
| ! | TGTTCTTGCGATAGTTTTTG+AGG | - | chr1_1:15246972-15246991 | None:intergenic | 35.0% |
| CAAAAACTATCGCAAGAACA+TGG | + | chr1_1:15246972-15246991 | Msa0010190:CDS | 35.0% | |
| CAACATTCAACTCCAGCAAT+GGG | + | chr1_1:15246697-15246716 | Msa0010190:CDS | 40.0% | |
| TCATCAGTGATTGTTGTGTC+TGG | - | chr1_1:15246781-15246800 | None:intergenic | 40.0% | |
| !! | GATGTTCTACATTGGCTTGT+TGG | - | chr1_1:15246867-15246886 | None:intergenic | 40.0% |
| AACTATCGCAAGAACATGGA+TGG | + | chr1_1:15246976-15246995 | Msa0010190:CDS | 40.0% | |
| ACTATCGCAAGAACATGGAT+GGG | + | chr1_1:15246977-15246996 | Msa0010190:CDS | 40.0% | |
| TCAACGTTGGTCATCATCAT+TGG | - | chr1_1:15247028-15247047 | None:intergenic | 40.0% | |
| ACAACCACCAACAACAACAC+TGG | + | chr1_1:15246658-15246677 | Msa0010190:CDS | 45.0% | |
| ! | AATGCCAGTGTTGTTGTTGG+TGG | - | chr1_1:15246665-15246684 | None:intergenic | 45.0% |
| ACCAACAACAACACTGGCAT+TGG | + | chr1_1:15246664-15246683 | Msa0010190:CDS | 45.0% | |
| ! | TCCAATGCCAGTGTTGTTGT+TGG | - | chr1_1:15246668-15246687 | None:intergenic | 45.0% |
| CCATTGCTGGAGTTGAATGT+TGG | - | chr1_1:15246699-15246718 | None:intergenic | 45.0% | |
| CCAACATTCAACTCCAGCAA+TGG | + | chr1_1:15246696-15246715 | Msa0010190:CDS | 45.0% | |
| GACTCTTGTCACATGACTGA+TGG | - | chr1_1:15246740-15246759 | None:intergenic | 45.0% | |
| TCAGTGATTGTTGTGTCTGG+TGG | - | chr1_1:15246778-15246797 | None:intergenic | 45.0% | |
| TAAGGACAAGGTCACAAGTG+AGG | + | chr1_1:15246837-15246856 | Msa0010190:CDS | 45.0% | |
| CGCCCATAGTAAATCATCAG+CGG | - | chr1_1:15246902-15246921 | None:intergenic | 45.0% | |
| ACTATGGGCGATGAATAGGT+TGG | + | chr1_1:15246912-15246931 | Msa0010190:CDS | 45.0% | |
| CTATGGGCGATGAATAGGTT+GGG | + | chr1_1:15246913-15246932 | Msa0010190:CDS | 45.0% | |
| ! | GATGAATAGGTTGGGCTTTG+AGG | + | chr1_1:15246921-15246940 | Msa0010190:CDS | 45.0% |
| !! | TGGGCTTTGAGGAACATGTT+CGG | + | chr1_1:15246932-15246951 | Msa0010190:CDS | 45.0% |
| AGGAACATGTTCGGTCTCTT+GGG | + | chr1_1:15246941-15246960 | Msa0010190:CDS | 45.0% | |
| ! | CAACACTGGCATTGGAACTG+AGG | + | chr1_1:15246672-15246691 | Msa0010190:CDS | 50.0% |
| GAGGAACATGTTCGGTCTCT+TGG | + | chr1_1:15246940-15246959 | Msa0010190:CDS | 50.0% | |
| TTCAACTCCAGCAATGGGCG+AGG | + | chr1_1:15246702-15246721 | Msa0010190:CDS | 55.0% | |
| CGCAAGAACATGGATGGGTC+AGG | + | chr1_1:15246982-15247001 | Msa0010190:CDS | 55.0% | |
| ATGGATGGGTCAGGTGCTAG+TGG | + | chr1_1:15246991-15247010 | Msa0010190:CDS | 55.0% | |
| CGCATTTCCTCGCCCATTGC+TGG | - | chr1_1:15246712-15246731 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 15246432 | 15247047 | 15246432 | ID=Msa0010190;Name=Msa0010190 |
| chr1_1 | mRNA | 15246432 | 15247047 | 15246432 | ID=Msa0010190-mRNA-1;Parent=Msa0010190;Name=Msa0010190-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|0.33|0|0.33|3|0|137 |
| chr1_1 | exon | 15246432 | 15246437 | 15246432 | ID=Msa0010190-mRNA-1:exon:5612;Parent=Msa0010190-mRNA-1 |
| chr1_1 | exon | 15246456 | 15246462 | 15246456 | ID=Msa0010190-mRNA-1:exon:5613;Parent=Msa0010190-mRNA-1 |
| chr1_1 | exon | 15246647 | 15247047 | 15246647 | ID=Msa0010190-mRNA-1:exon:5614;Parent=Msa0010190-mRNA-1 |
| chr1_1 | CDS | 15246432 | 15246437 | 15246432 | ID=Msa0010190-mRNA-1:cds;Parent=Msa0010190-mRNA-1 |
| chr1_1 | CDS | 15246456 | 15246462 | 15246456 | ID=Msa0010190-mRNA-1:cds;Parent=Msa0010190-mRNA-1 |
| chr1_1 | CDS | 15246647 | 15247047 | 15246647 | ID=Msa0010190-mRNA-1:cds;Parent=Msa0010190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
