AlfalfaGEDB | Msa0011260

M. sativa cultivar zhongmu-4 / Msa0011260

KEGG: K10523
GO: GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031461 GO:0031463 GO:0031625 GO:0032991 GO:0043161 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234
PFAM: PF00651.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011260	sp\|Q9XHZ8\|Y1178_ARATH	71.646	328	89	1	1	328	1	324	0.0	507

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0011260	TR	TRAF

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011260	MtrunA17_Chr1g0159831	98.182	330	4	1	1	328	1	330	0.0	671
Msa0011260	MtrunA17_Chr3g0124921	72.866	328	82	4	1	326	1	323	1.69e-176	491
Msa0011260	MtrunA17_Chr3g0103031	50.755	331	157	3	1	328	1	328	1.99e-119	347
Msa0011260	MtrunA17_Chr5g0447111	44.615	325	175	3	5	328	9	329	1.47e-98	294
Msa0011260	MtrunA17_Chr5g0447191	39.572	187	109	2	140	324	3	187	1.36e-41	143
Msa0011260	MtrunA17_Chr4g0029841	30.723	166	107	2	160	325	122	279	8.25e-21	90.5
Msa0011260	MtrunA17_Chr7g0259501	29.448	163	102	3	142	291	182	344	4.82e-11	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011260	AT1G21780.2	71.646	328	89	1	1	328	1	324	0.0	507
Msa0011260	AT1G21780.1	71.646	328	89	1	1	328	1	324	0.0	507
Msa0011260	AT1G55760.1	47.892	332	164	5	1	328	1	327	1.80e-109	322
Msa0011260	AT2G39760.4	30.719	153	95	1	150	291	179	331	2.85e-13	70.1
Msa0011260	AT2G39760.3	30.719	153	95	1	150	291	179	331	2.85e-13	70.1
Msa0011260	AT2G39760.2	30.719	153	95	1	150	291	179	331	3.02e-13	70.1
Msa0011260	AT2G39760.1	30.719	153	95	1	150	291	179	331	6.20e-13	69.3

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Find 83 sgRNAs with CRISPR-Local

Find 319 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAAACAAAACAAAACA+TGG	0.565821	1_1:+16985975	Msa0011260:exon
AAATCAGACCCTTTCAAACT+TGG	0.408826	1_1:+16986072	Msa0011260:CDS
AACAAAACAAAACAAAACAT+GGG	0.638409	1_1:+16985976	Msa0011260:exon
AACCGCAGATGGCACACTTA+AGG	0.459877	1_1:+16989325	Msa0011260:CDS
AAGCACAGGCTGGCTTTGCT+TGG	0.389834	1_1:+16989515	Msa0011260:CDS
AATTAGAGACTCACATTCAG+AGG	0.576553	1_1:-16988923	None:intergenic
ACAAAACATGGGAGATTCGA+AGG	0.560639	1_1:+16985987	Msa0011260:exon
ACCCTTTCAAACTTGGTATT+TGG	0.128764	1_1:+16986079	Msa0011260:CDS
ACTCGGGAAATGTTCTCGAG+AGG	0.641157	1_1:+16989606	Msa0011260:CDS
AGAAGAAGAAGAAGCAGTAT+TGG	0.384918	1_1:-16986296	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTGATTTCTTGTAAGAACA+TGG	-	chr1_1:16986060-16986079	None:intergenic	30.0%
TCCAAATACCAAGTTTGAAA+GGG	-	chr1_1:16986083-16986102	None:intergenic	30.0%
TTCCAAATACCAAGTTTGAA+AGG	-	chr1_1:16986084-16986103	None:intergenic	30.0%
AAGAGATAACATACTAACCA+GGG	-	chr1_1:16986345-16986364	None:intergenic	30.0%
TAAGAGATAACATACTAACC+AGG	-	chr1_1:16986346-16986365	None:intergenic	30.0%
AGACACTCTTTAATCAATGT+GGG	-	chr1_1:16986486-16986505	None:intergenic	30.0%
AAAGGAGTAAACCATCAAAT+TGG	+	chr1_1:16986517-16986536	Msa0011260:intron	30.0%
AATCCTACTAGTAAAACTCA+AGG	-	chr1_1:16986755-16986774	None:intergenic	30.0%
AATAAGAAGTTTAGGTGAGT+TGG	+	chr1_1:16986792-16986811	Msa0011260:intron	30.0%
ATAAGAAGTTTAGGTGAGTT+GGG	+	chr1_1:16986793-16986812	Msa0011260:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	16985808	16990015	16985808	ID=Msa0011260;Name=Msa0011260
chr1_1	mRNA	16985808	16990015	16985808	ID=Msa0011260-mRNA-1;Parent=Msa0011260;Name=Msa0011260-mRNA-1;_AED=0.11;_eAED=0.11;_QI=186\|1\|1\|1\|1\|1\|4\|213\|328
chr1_1	exon	16985808	16986106	16985808	ID=Msa0011260-mRNA-1:exon:6207;Parent=Msa0011260-mRNA-1
chr1_1	exon	16986189	16986346	16986189	ID=Msa0011260-mRNA-1:exon:6208;Parent=Msa0011260-mRNA-1
chr1_1	exon	16988816	16988931	16988816	ID=Msa0011260-mRNA-1:exon:6209;Parent=Msa0011260-mRNA-1
chr1_1	exon	16989203	16990015	16989203	ID=Msa0011260-mRNA-1:exon:6210;Parent=Msa0011260-mRNA-1
chr1_1	five_prime_UTR	16985808	16985993	16985808	ID=Msa0011260-mRNA-1:five_prime_utr;Parent=Msa0011260-mRNA-1
chr1_1	CDS	16985994	16986106	16985994	ID=Msa0011260-mRNA-1:cds;Parent=Msa0011260-mRNA-1
chr1_1	CDS	16986189	16986346	16986189	ID=Msa0011260-mRNA-1:cds;Parent=Msa0011260-mRNA-1
chr1_1	CDS	16988816	16988931	16988816	ID=Msa0011260-mRNA-1:cds;Parent=Msa0011260-mRNA-1
chr1_1	CDS	16989203	16989802	16989203	ID=Msa0011260-mRNA-1:cds;Parent=Msa0011260-mRNA-1
chr1_1	three_prime_UTR	16989803	16990015	16989803	ID=Msa0011260-mRNA-1:three_prime_utr;Parent=Msa0011260-mRNA-1

Gene Sequence

>Msa0011260

ATGGGAGATTCGAAGGTAGAAACAATTTCCCGATTAGCTCAATGGAAAATCGACAATTTTGGACCATGTTCTTACAAGAAATCAGACCCTTTCAAACTTGGTATTTGGAACTGGTATTTGTCGATAGAGAGAAACAGGTATTTGTATATTCATCTATTTCCTGAACCTTCGCGTGTTTCTAAGGAACAGCCTCCTATTGCTCGTTTCATTCTTCGTGTTTCCAATACTGCTTCTTCTTCTTCTTCCTCCCGCAAATTTTACATTTCCCCTGTTCATGAAAGGGTGCTTAGAACCTGTGATGACTTTGTCTGGCCTGTGGATACTACATTCCTTGGTCGCTTGATTATTGATGTTGAATTTCTTGACCTCAAGATCCATCCTCTGAATGGTGGAGAAGCCAGTTCCATATGGCCATCTGATGGAAAACTGCATTCTGTTGCAACTCAAAACACTCTCCGATGCCTCTCTCGTATGCTTGACAAAGCTATACATGTTGACCTCACTATTATAACCGCAGATGGCACACTTAAGGCTCATAAAGCAGTTATGTCCGCAACCTCTCCTGTGTTTCAGGCCTCGTATCACAGCAGCAGTGAAGACAAAGAGTCGTCTACAATCCACATAGAAGACATGTCACAAGAATCCTGCACGGCCCTTCTGAGATACATGTATGGAACAATAAAACCCGAAGATTTCTGGAAGCACAGGCTGGCTTTGCTTGGAGCAGCTAATAAGTACGACATCGGTGATCTCAAAGACGCATGCGAGGAAAGCCTACTTGAAGATCTTAACTCGGGAAATGTTCTCGAGAGGCTTAATGAGGCTTGGTTGTACCAGCTGCACAAGTTGAAGAAAGGATGCTTCGCATTCTTATTTGATTTCGGTAAGATATATGATGTTAGAGATGAAATAAATACGTTTTTTCGTCATGCAGACAGGGAGCTCATGCTAGAGATGTTTCAAGAAGTGCTTACAATTTGGAAATAG

Protein sequence

>Msa0011260

MGDSKVETISRLAQWKIDNFGPCSYKKSDPFKLGIWNWYLSIERNRYLYIHLFPEPSRVSKEQPPIARFILRVSNTASSSSSSRKFYISPVHERVLRTCDDFVWPVDTTFLGRLIIDVEFLDLKIHPLNGGEASSIWPSDGKLHSVATQNTLRCLSRMLDKAIHVDLTIITADGTLKAHKAVMSATSPVFQASYHSSSEDKESSTIHIEDMSQESCTALLRYMYGTIKPEDFWKHRLALLGAANKYDIGDLKDACEESLLEDLNSGNVLERLNEAWLYQLHKLKKGCFAFLFDFGKIYDVRDEINTFFRHADRELMLEMFQEVLTIWK*