Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0011780 | sp|P93604|LRK10_WHEAT | 55.556 | 315 | 136 | 3 | 5 | 316 | 314 | 627 | 4.01e-120 | 363 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0011780 | MtrunA17_Chr1g0160561 | 88.286 | 350 | 40 | 1 | 1 | 350 | 317 | 665 | 0.0 | 644 |
| Msa0011780 | MtrunA17_Chr1g0160531 | 86.571 | 350 | 45 | 2 | 1 | 350 | 95 | 442 | 0.0 | 628 |
| Msa0011780 | MtrunA17_Chr1g0160721 | 86.533 | 349 | 43 | 2 | 1 | 349 | 322 | 666 | 0.0 | 624 |
| Msa0011780 | MtrunA17_Chr1g0160701 | 82.857 | 350 | 59 | 1 | 1 | 350 | 316 | 664 | 0.0 | 584 |
| Msa0011780 | MtrunA17_Chr1g0160761 | 82.133 | 347 | 58 | 2 | 1 | 347 | 306 | 648 | 0.0 | 572 |
| Msa0011780 | MtrunA17_Chr1g0160711 | 78.797 | 349 | 68 | 2 | 1 | 349 | 299 | 641 | 0.0 | 545 |
| Msa0011780 | MtrunA17_Chr1g0158761 | 66.768 | 328 | 106 | 3 | 2 | 328 | 306 | 631 | 3.73e-159 | 461 |
| Msa0011780 | MtrunA17_Chr1g0157811 | 64.265 | 347 | 118 | 4 | 1 | 343 | 171 | 515 | 7.83e-159 | 455 |
| Msa0011780 | MtrunA17_Chr1g0157951 | 65.951 | 326 | 109 | 2 | 1 | 326 | 152 | 475 | 9.28e-159 | 454 |
| Msa0011780 | MtrunA17_Chr3g0088321 | 62.644 | 348 | 127 | 3 | 2 | 349 | 26 | 370 | 1.95e-155 | 441 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0011780 | AT5G38280.1 | 48.137 | 322 | 159 | 5 | 19 | 335 | 320 | 638 | 2.97e-104 | 320 |
| Msa0011780 | AT5G38280.2 | 48.137 | 322 | 159 | 5 | 19 | 335 | 320 | 638 | 4.89e-104 | 320 |
| Msa0011780 | AT4G18250.1 | 49.515 | 309 | 146 | 4 | 15 | 315 | 340 | 646 | 1.93e-103 | 319 |
| Msa0011780 | AT5G38240.3 | 51.572 | 318 | 136 | 6 | 9 | 318 | 247 | 554 | 2.61e-103 | 315 |
| Msa0011780 | AT5G38250.1 | 53.135 | 303 | 129 | 5 | 20 | 318 | 304 | 597 | 6.61e-103 | 315 |
| Msa0011780 | AT5G38240.2 | 51.572 | 318 | 136 | 6 | 9 | 318 | 293 | 600 | 1.52e-102 | 315 |
| Msa0011780 | AT5G38240.1 | 52.787 | 305 | 129 | 5 | 19 | 318 | 324 | 618 | 2.57e-102 | 315 |
| Msa0011780 | AT5G38260.1 | 47.748 | 333 | 163 | 5 | 11 | 335 | 298 | 627 | 1.44e-101 | 313 |
| Msa0011780 | AT1G66920.1 | 47.416 | 329 | 160 | 5 | 19 | 344 | 288 | 606 | 3.73e-101 | 311 |
| Msa0011780 | AT1G66920.2 | 47.416 | 329 | 160 | 5 | 19 | 344 | 296 | 614 | 4.08e-101 | 311 |
Find 80 sgRNAs with CRISPR-Local
Find 166 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCTTGACCGTCGGACT+TGG | 0.452034 | 1_1:+17718330 | None:intergenic |
| AAACAATTCAAGACAAAACT+AGG | 0.538665 | 1_1:-17718430 | Msa0011780:CDS |
| AAACATTTATCAATGTACGA+CGG | 0.729408 | 1_1:-17718529 | None:intergenic |
| AAACTAGGAAGTGGAGGTTA+TGG | 0.417571 | 1_1:-17718415 | Msa0011780:CDS |
| AAAGCTGATGTTTATAGCTT+TGG | 0.271856 | 1_1:-17717878 | Msa0011780:CDS |
| AAATGATGATCGTGGCTTTG+TGG | 0.564962 | 1_1:-17717694 | Msa0011780:CDS |
| AAATTGCATGCTATTTCTCT+TGG | 0.377857 | 1_1:-17718136 | Msa0011780:CDS |
| AACTAGGAAGTGGAGGTTAT+GGG | 0.439959 | 1_1:-17718414 | Msa0011780:CDS |
| AAGACAAAACTAGGAAGTGG+AGG | 0.627937 | 1_1:-17718421 | Msa0011780:CDS |
| AAGAGATTAGGCTGGTCCCT+TGG | 0.457251 | 1_1:+17717481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGATAAAATCTTGACCGT+CGG | + | chr1_1:17717683-17717702 | None:intergenic | 30.0% | |
| ATCAATGAAATTGCTACCAT+TGG | - | chr1_1:17717697-17717716 | Msa0011780:CDS | 30.0% | |
| AAAGCTGATGTTTATAGCTT+TGG | - | chr1_1:17718126-17718145 | Msa0011780:CDS | 30.0% | |
| AAGCTGATGTTTATAGCTTT+GGG | - | chr1_1:17718127-17718146 | Msa0011780:CDS | 30.0% | |
| AAGGAAGAATTTGAATGCAT+TGG | - | chr1_1:17718176-17718195 | Msa0011780:CDS | 30.0% | |
| TCCAGAAAGGGAAATAAATT+TGG | + | chr1_1:17718215-17718234 | None:intergenic | 30.0% | |
| ATCGATCATAAATCCAGAAA+GGG | + | chr1_1:17718227-17718246 | None:intergenic | 30.0% | |
| AATCGATCATAAATCCAGAA+AGG | + | chr1_1:17718228-17718247 | None:intergenic | 30.0% | |
| TATTGAAAATGACACAGATC+AGG | - | chr1_1:17718269-17718288 | Msa0011780:CDS | 30.0% | |
| TCCAAAACTTTATCCATTGA+AGG | + | chr1_1:17718363-17718382 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 17717487 | 17718539 | 17717487 | ID=Msa0011780;Name=Msa0011780 |
| chr1_1 | mRNA | 17717487 | 17718539 | 17717487 | ID=Msa0011780-mRNA-1;Parent=Msa0011780;Name=Msa0011780-mRNA-1;_AED=0.43;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|350 |
| chr1_1 | exon | 17717487 | 17718539 | 17717487 | ID=Msa0011780-mRNA-1:exon:6506;Parent=Msa0011780-mRNA-1 |
| chr1_1 | CDS | 17717487 | 17718539 | 17717487 | ID=Msa0011780-mRNA-1:cds;Parent=Msa0011780-mRNA-1 |
| Gene Sequence |
>Msa0011780 ATGTACGACGGCATTGAAAGTTTTCTACGCCGCGACAATAGCATCATGCCAGTAAGGTACTCTTACAAAGACATAAAAAAGATAACAAAACAATTCAAGACAAAACTAGGAAGTGGAGGTTATGGGTCAGTTTTCAAAGGACAACTTCGAAGTGGTCGTCTTGTAGCAGTAAAATTATTGGACATAGCCAAGTCCGACGGTCAAGATTTTATCAATGAAATTGCTACCATTGGTAGGATTCACCATCTTAATGTGGTACAACTCATTGGTTTTTGTGTGGAGGGGTCAAAACGTGCTCTTATATATGAATTCATGCCAAATGGATCACTCGAAAAATACATATTTTCTCATATTGAAGAGAGTAATTCTTTGGGTTGCAAAAAATTGCATGCTATTTCTCTTGGAGTAGCTCGAGGCATTGAGTACTTGCATAATGGATGCAATATGAAAATTCTACACTTTGATATAAAGTCTCACAACATCCTTCTTGATGAGAATTTTAATCCAAAAGTTTCTGATTTCGGACTAGCTAGACTTTGTCCTACAGGTAATAGCATTGTGTCATTAACGGCTGCAAGAGGAACCATTGGATATATGGCCCCTGAACTATTCTATAGAAATGTTGGCACAATATCTTACAAAGCTGATGTTTATAGCTTTGGGATGTTGTTAATGGAGATGGCAAGTAGAAGGAAGAATTTGAATGCATTGGCCGATCAATCTAGCCAAATTTATTTCCCTTTCTGGATTTATGATCGATTACATGATGGAAGGGAAGTATCTATTGAAAATGACACAGATCAGGAAATGAAATTGGCAAAGAAAATGATGATCGTGGCTTTGTGGTGTATACAAACAAAACCCGAGGATCGTCCTTCAATGGATAAAGTTTTGGAGATGCTTGAAGAAGAAGAAGATGGGGTGTTGCAAATTCCTAATAAGCCTTACTTTTATCCACAAGATCCACCAGTAGCGGATGTTGGAGTTGATAATAGTAGCAACTCATGGACTTCTTATGGTACATCAGTTAGTGATCCCAAGGGACCAGCCTAA |
| Protein sequence |
>Msa0011780 MYDGIESFLRRDNSIMPVRYSYKDIKKITKQFKTKLGSGGYGSVFKGQLRSGRLVAVKLLDIAKSDGQDFINEIATIGRIHHLNVVQLIGFCVEGSKRALIYEFMPNGSLEKYIFSHIEESNSLGCKKLHAISLGVARGIEYLHNGCNMKILHFDIKSHNILLDENFNPKVSDFGLARLCPTGNSIVSLTAARGTIGYMAPELFYRNVGTISYKADVYSFGMLLMEMASRRKNLNALADQSSQIYFPFWIYDRLHDGREVSIENDTDQEMKLAKKMMIVALWCIQTKPEDRPSMDKVLEMLEEEEDGVLQIPNKPYFYPQDPPVADVGVDNSSNSWTSYGTSVSDPKGPA* |
