AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0012860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0012860 MtrunA17_Chr1g0161901 92.636 258 12 2 1 258 1 251 1.23e-177 488
Msa0012860 MtrunA17_Chr8g0376021 51.751 257 84 8 2 258 1 217 5.95e-75 227
Msa0012860 MtrunA17_Chr3g0110091 40.157 254 134 6 5 258 3 238 1.16e-56 181
Msa0012860 MtrunA17_Chr5g0434121 43.609 266 105 11 6 258 4 237 5.19e-55 177
Msa0012860 MtrunA17_Chr2g0333741 66.234 77 21 2 98 169 81 157 3.12e-26 102
Msa0012860 MtrunA17_Chr1g0149811 38.356 146 71 2 43 169 35 180 1.72e-23 95.9
Msa0012860 MtrunA17_Chr4g0001081 58.108 74 19 3 98 169 67 130 6.56e-19 82.8
Msa0012860 MtrunA17_Chr1g0207691 38.889 126 59 4 98 219 64 175 1.13e-18 81.3
Msa0012860 MtrunA17_Chr3g0137111 43.103 116 45 5 69 169 49 158 3.59e-18 80.9
Msa0012860 MtrunA17_Chr4g0072571 52.632 76 32 2 98 171 45 118 4.34e-16 73.9
Msa0012860 MtrunA17_Chr4g0053691 36.364 143 66 7 90 229 57 177 7.50e-15 70.9
Msa0012860 MtrunA17_Chr7g0254221 52.830 53 23 2 82 134 19 69 1.06e-11 61.6
Msa0012860 MtrunA17_Chr7g0251581 69.697 33 10 0 98 130 39 71 2.16e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0012860 AT1G66140.1 41.758 182 81 6 98 258 83 260 3.51e-37 132
Msa0012860 AT1G24625.1 39.207 227 95 10 39 258 19 209 2.59e-31 115
Msa0012860 AT1G80730.1 57.955 88 21 3 98 169 66 153 9.79e-24 96.3
Msa0012860 AT5G10970.1 56.579 76 29 2 97 169 101 175 1.75e-21 90.9
Msa0012860 AT5G25160.1 55.263 76 30 2 98 169 59 134 6.93e-21 88.6
Msa0012860 AT5G57520.1 51.515 66 32 0 98 163 50 115 1.65e-15 72.0
Msa0012860 AT5G14010.1 47.945 73 27 1 94 155 32 104 2.40e-15 72.0
Msa0012860 AT5G01860.1 55.814 43 19 0 95 137 62 104 4.20e-11 61.2
Msa0012860 AT3G58070.1 55.769 52 20 1 82 133 76 124 7.30e-11 61.2

Find 46 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTTCCAAGTTCAAATT+TGG 0.203450 1_1:-19798130 None:intergenic
ATGAAGATGTCGGCGTATTT+TGG 0.224650 1_1:+19798731 Msa0012860:CDS
GACATCAGAATGCTCATAAA+AGG 0.253478 1_1:+19798467 Msa0012860:CDS
TTAGCTTCTTTACCTCTTCA+TGG 0.299313 1_1:+19798550 Msa0012860:CDS
AGAAGTTGTGGCACTGTCTT+TGG 0.311560 1_1:-19798221 None:intergenic
ACGATGATGGCATGTGGTTT+TGG 0.333669 1_1:-19798621 None:intergenic
TTTATCTGCCTGAAACTTCC+AGG 0.347691 1_1:-19798754 None:intergenic
AAATTCGAGAAAGATTGTTT+TGG 0.369690 1_1:+19798685 Msa0012860:CDS
CATATCCTGATCTTACTTTG+AGG 0.427108 1_1:+19798866 Msa0012860:CDS
TCCGAGCGATGAAGATTTGA+AGG 0.428555 1_1:+19798309 Msa0012860:CDS
TCCTTCAAATCTTCATCGCT+CGG 0.432671 1_1:-19798310 None:intergenic
AACTTCTTCTTGTCTTACTA+AGG 0.451364 1_1:+19798237 Msa0012860:CDS
GAATACTCCAAATTTGAACT+TGG 0.473476 1_1:+19798123 Msa0012860:CDS
TCGGATTGAAGTTGAGAGTC+AGG 0.475494 1_1:-19798291 None:intergenic
TTCTTTAGCTCACAAGCGTT+AGG 0.479990 1_1:+19798442 Msa0012860:CDS
CAAAGCGCGCTATGCGAGTA+GGG 0.487145 1_1:+19798503 Msa0012860:CDS
ACTAAGGCAGACATTAATCC+AGG 0.488717 1_1:+19798253 Msa0012860:CDS
AGCTAAGCTTGTATACCCTT+CGG 0.491197 1_1:-19798533 None:intergenic
GTAGTAGATGTTTGTTGAGG+AGG 0.505399 1_1:-19798844 None:intergenic
TAAAGCCTCAAAGTAAGATC+AGG 0.515851 1_1:-19798871 None:intergenic
TCTCTTGTAATTATTGTAGG+AGG 0.522769 1_1:+19798416 Msa0012860:CDS
ATCAGCATTGACGACGATGA+TGG 0.524569 1_1:-19798634 None:intergenic
TGATTCTTGTAAGTATATGT+TGG 0.525252 1_1:-19798185 None:intergenic
GAGAAAACTCTATGAATCGC+TGG 0.540223 1_1:-19798397 None:intergenic
GCAAAGCGCGCTATGCGAGT+AGG 0.540707 1_1:+19798502 Msa0012860:CDS
AGGTCAAGGGAAATAGAACC+TGG 0.542650 1_1:-19798271 None:intergenic
ACATCAGAATGCTCATAAAA+GGG 0.543025 1_1:+19798468 Msa0012860:CDS
AGTATATGTTGGAACCTACT+TGG 0.544787 1_1:-19798174 None:intergenic
TCTTTCTCGAATTTCGCTGT+AGG 0.553624 1_1:-19798676 None:intergenic
AACTCTATGAATCGCTGGTG+CGG 0.556385 1_1:-19798392 None:intergenic
CATCGTCGTCAATGCTGATG+AGG 0.567172 1_1:+19798638 Msa0012860:CDS
TATGTAGTAGATGTTTGTTG+AGG 0.576983 1_1:-19798847 None:intergenic
TTTAGCTCACAAGCGTTAGG+TGG 0.582305 1_1:+19798445 Msa0012860:CDS
TGAAGTTGAGAGTCAGGTCA+AGG 0.584371 1_1:-19798285 None:intergenic
TTAGAAGTAAGCAGCCAAGT+AGG 0.598009 1_1:+19798160 Msa0012860:CDS
CGAGTAGGGATGTTTACCGA+AGG 0.601367 1_1:+19798517 Msa0012860:CDS
ATCGTCGTCAATGCTGATGA+GGG 0.604838 1_1:+19798639 Msa0012860:CDS
GACACTAACAGTGAAGTACT+AGG 0.613028 1_1:+19798340 Msa0012860:CDS
AAAGAAGAAGAACCATGAAG+AGG 0.622542 1_1:-19798562 None:intergenic
GAAGTTGAGAGTCAGGTCAA+GGG 0.634039 1_1:-19798284 None:intergenic
TTTATGCATAATGAAGATGT+CGG 0.651635 1_1:+19798721 Msa0012860:CDS
AGTAAGACAAGAAGAAGTTG+TGG 0.657771 1_1:-19798233 None:intergenic
GAGTAGGGATGTTTACCGAA+GGG 0.666419 1_1:+19798518 Msa0012860:CDS
TCTTCATTATGCATAAACAT+CGG 0.674163 1_1:-19798715 None:intergenic
TCATAAAAGGGAGAGAACGA+TGG 0.677286 1_1:+19798480 Msa0012860:CDS
TTGACGACGATGATGGCATG+TGG 0.711687 1_1:-19798627 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTCTTGTAATTATTGT+AGG + chr1_1:19798413-19798432 Msa0012860:CDS 20.0%
!!! TTTTCTCTTGTAATTATTGT+AGG + chr1_1:19798413-19798432 Msa0012860:CDS 20.0%
! TGATTCTTGTAAGTATATGT+TGG - chr1_1:19798188-19798207 None:intergenic 25.0%
!! AAATTCGAGAAAGATTGTTT+TGG + chr1_1:19798685-19798704 Msa0012860:CDS 25.0%
! TCTTCATTATGCATAAACAT+CGG - chr1_1:19798718-19798737 None:intergenic 25.0%
! TTTATGCATAATGAAGATGT+CGG + chr1_1:19798721-19798740 Msa0012860:CDS 25.0%
! TGATTCTTGTAAGTATATGT+TGG - chr1_1:19798188-19798207 None:intergenic 25.0%
!! AAATTCGAGAAAGATTGTTT+TGG + chr1_1:19798685-19798704 Msa0012860:CDS 25.0%
! TCTTCATTATGCATAAACAT+CGG - chr1_1:19798718-19798737 None:intergenic 25.0%
! TTTATGCATAATGAAGATGT+CGG + chr1_1:19798721-19798740 Msa0012860:CDS 25.0%
GAATACTCCAAATTTGAACT+TGG + chr1_1:19798123-19798142 Msa0012860:CDS 30.0%
AACTTCTTCTTGTCTTACTA+AGG + chr1_1:19798237-19798256 Msa0012860:CDS 30.0%
TCTCTTGTAATTATTGTAGG+AGG + chr1_1:19798416-19798435 Msa0012860:CDS 30.0%
ACATCAGAATGCTCATAAAA+GGG + chr1_1:19798468-19798487 Msa0012860:CDS 30.0%
! TCTTCTTCTTTTCATTCTCT+TGG + chr1_1:19798574-19798593 Msa0012860:CDS 30.0%
! TATGTAGTAGATGTTTGTTG+AGG - chr1_1:19798850-19798869 None:intergenic 30.0%
GAATACTCCAAATTTGAACT+TGG + chr1_1:19798123-19798142 Msa0012860:CDS 30.0%
AACTTCTTCTTGTCTTACTA+AGG + chr1_1:19798237-19798256 Msa0012860:CDS 30.0%
TCTCTTGTAATTATTGTAGG+AGG + chr1_1:19798416-19798435 Msa0012860:CDS 30.0%
ACATCAGAATGCTCATAAAA+GGG + chr1_1:19798468-19798487 Msa0012860:CDS 30.0%
! TCTTCTTCTTTTCATTCTCT+TGG + chr1_1:19798574-19798593 Msa0012860:CDS 30.0%
! TATGTAGTAGATGTTTGTTG+AGG - chr1_1:19798850-19798869 None:intergenic 30.0%
TCAGCTTCCAAGTTCAAATT+TGG - chr1_1:19798133-19798152 None:intergenic 35.0%
AGTATATGTTGGAACCTACT+TGG - chr1_1:19798177-19798196 None:intergenic 35.0%
AGTAAGACAAGAAGAAGTTG+TGG - chr1_1:19798236-19798255 None:intergenic 35.0%
GACATCAGAATGCTCATAAA+AGG + chr1_1:19798467-19798486 Msa0012860:CDS 35.0%
TTAGCTTCTTTACCTCTTCA+TGG + chr1_1:19798550-19798569 Msa0012860:CDS 35.0%
AAAGAAGAAGAACCATGAAG+AGG - chr1_1:19798565-19798584 None:intergenic 35.0%
CATATCCTGATCTTACTTTG+AGG + chr1_1:19798866-19798885 Msa0012860:CDS 35.0%
TAAAGCCTCAAAGTAAGATC+AGG - chr1_1:19798874-19798893 None:intergenic 35.0%
TCAGCTTCCAAGTTCAAATT+TGG - chr1_1:19798133-19798152 None:intergenic 35.0%
AGTATATGTTGGAACCTACT+TGG - chr1_1:19798177-19798196 None:intergenic 35.0%
AGTAAGACAAGAAGAAGTTG+TGG - chr1_1:19798236-19798255 None:intergenic 35.0%
GACATCAGAATGCTCATAAA+AGG + chr1_1:19798467-19798486 Msa0012860:CDS 35.0%
TTAGCTTCTTTACCTCTTCA+TGG + chr1_1:19798550-19798569 Msa0012860:CDS 35.0%
AAAGAAGAAGAACCATGAAG+AGG - chr1_1:19798565-19798584 None:intergenic 35.0%
CATATCCTGATCTTACTTTG+AGG + chr1_1:19798866-19798885 Msa0012860:CDS 35.0%
TAAAGCCTCAAAGTAAGATC+AGG - chr1_1:19798874-19798893 None:intergenic 35.0%
TTAGAAGTAAGCAGCCAAGT+AGG + chr1_1:19798160-19798179 Msa0012860:CDS 40.0%
ACTAAGGCAGACATTAATCC+AGG + chr1_1:19798253-19798272 Msa0012860:CDS 40.0%
TCCTTCAAATCTTCATCGCT+CGG - chr1_1:19798313-19798332 None:intergenic 40.0%
! GACACTAACAGTGAAGTACT+AGG + chr1_1:19798340-19798359 Msa0012860:CDS 40.0%
GAGAAAACTCTATGAATCGC+TGG - chr1_1:19798400-19798419 None:intergenic 40.0%
! TTCTTTAGCTCACAAGCGTT+AGG + chr1_1:19798442-19798461 Msa0012860:CDS 40.0%
TCATAAAAGGGAGAGAACGA+TGG + chr1_1:19798480-19798499 Msa0012860:CDS 40.0%
AGCTAAGCTTGTATACCCTT+CGG - chr1_1:19798536-19798555 None:intergenic 40.0%
TCTTTCTCGAATTTCGCTGT+AGG - chr1_1:19798679-19798698 None:intergenic 40.0%
! ATGAAGATGTCGGCGTATTT+TGG + chr1_1:19798731-19798750 Msa0012860:CDS 40.0%
!!! TATTTTGGCCTGGAAGTTTC+AGG + chr1_1:19798746-19798765 Msa0012860:CDS 40.0%
TTTATCTGCCTGAAACTTCC+AGG - chr1_1:19798757-19798776 None:intergenic 40.0%
! GTAGTAGATGTTTGTTGAGG+AGG - chr1_1:19798847-19798866 None:intergenic 40.0%
TTAGAAGTAAGCAGCCAAGT+AGG + chr1_1:19798160-19798179 Msa0012860:CDS 40.0%
ACTAAGGCAGACATTAATCC+AGG + chr1_1:19798253-19798272 Msa0012860:CDS 40.0%
TCCTTCAAATCTTCATCGCT+CGG - chr1_1:19798313-19798332 None:intergenic 40.0%
! GACACTAACAGTGAAGTACT+AGG + chr1_1:19798340-19798359 Msa0012860:CDS 40.0%
GAGAAAACTCTATGAATCGC+TGG - chr1_1:19798400-19798419 None:intergenic 40.0%
! TTCTTTAGCTCACAAGCGTT+AGG + chr1_1:19798442-19798461 Msa0012860:CDS 40.0%
TCATAAAAGGGAGAGAACGA+TGG + chr1_1:19798480-19798499 Msa0012860:CDS 40.0%
AGCTAAGCTTGTATACCCTT+CGG - chr1_1:19798536-19798555 None:intergenic 40.0%
TCTTTCTCGAATTTCGCTGT+AGG - chr1_1:19798679-19798698 None:intergenic 40.0%
! ATGAAGATGTCGGCGTATTT+TGG + chr1_1:19798731-19798750 Msa0012860:CDS 40.0%
!!! TATTTTGGCCTGGAAGTTTC+AGG + chr1_1:19798746-19798765 Msa0012860:CDS 40.0%
TTTATCTGCCTGAAACTTCC+AGG - chr1_1:19798757-19798776 None:intergenic 40.0%
! GTAGTAGATGTTTGTTGAGG+AGG - chr1_1:19798847-19798866 None:intergenic 40.0%
AGAAGTTGTGGCACTGTCTT+TGG - chr1_1:19798224-19798243 None:intergenic 45.0%
AGGTCAAGGGAAATAGAACC+TGG - chr1_1:19798274-19798293 None:intergenic 45.0%
GAAGTTGAGAGTCAGGTCAA+GGG - chr1_1:19798287-19798306 None:intergenic 45.0%
TGAAGTTGAGAGTCAGGTCA+AGG - chr1_1:19798288-19798307 None:intergenic 45.0%
TCGGATTGAAGTTGAGAGTC+AGG - chr1_1:19798294-19798313 None:intergenic 45.0%
TCCGAGCGATGAAGATTTGA+AGG + chr1_1:19798309-19798328 Msa0012860:CDS 45.0%
AACTCTATGAATCGCTGGTG+CGG - chr1_1:19798395-19798414 None:intergenic 45.0%
! TTTAGCTCACAAGCGTTAGG+TGG + chr1_1:19798445-19798464 Msa0012860:CDS 45.0%
GAGTAGGGATGTTTACCGAA+GGG + chr1_1:19798518-19798537 Msa0012860:CDS 45.0%
!! ACGATGATGGCATGTGGTTT+TGG - chr1_1:19798624-19798643 None:intergenic 45.0%
!! ATCAGCATTGACGACGATGA+TGG - chr1_1:19798637-19798656 None:intergenic 45.0%
ATCGTCGTCAATGCTGATGA+GGG + chr1_1:19798639-19798658 Msa0012860:CDS 45.0%
AGAAGTTGTGGCACTGTCTT+TGG - chr1_1:19798224-19798243 None:intergenic 45.0%
AGGTCAAGGGAAATAGAACC+TGG - chr1_1:19798274-19798293 None:intergenic 45.0%
GAAGTTGAGAGTCAGGTCAA+GGG - chr1_1:19798287-19798306 None:intergenic 45.0%
TGAAGTTGAGAGTCAGGTCA+AGG - chr1_1:19798288-19798307 None:intergenic 45.0%
TCGGATTGAAGTTGAGAGTC+AGG - chr1_1:19798294-19798313 None:intergenic 45.0%
TCCGAGCGATGAAGATTTGA+AGG + chr1_1:19798309-19798328 Msa0012860:CDS 45.0%
AACTCTATGAATCGCTGGTG+CGG - chr1_1:19798395-19798414 None:intergenic 45.0%
! TTTAGCTCACAAGCGTTAGG+TGG + chr1_1:19798445-19798464 Msa0012860:CDS 45.0%
GAGTAGGGATGTTTACCGAA+GGG + chr1_1:19798518-19798537 Msa0012860:CDS 45.0%
!! ACGATGATGGCATGTGGTTT+TGG - chr1_1:19798624-19798643 None:intergenic 45.0%
!! ATCAGCATTGACGACGATGA+TGG - chr1_1:19798637-19798656 None:intergenic 45.0%
ATCGTCGTCAATGCTGATGA+GGG + chr1_1:19798639-19798658 Msa0012860:CDS 45.0%
CGAGTAGGGATGTTTACCGA+AGG + chr1_1:19798517-19798536 Msa0012860:CDS 50.0%
! TTGACGACGATGATGGCATG+TGG - chr1_1:19798630-19798649 None:intergenic 50.0%
CATCGTCGTCAATGCTGATG+AGG + chr1_1:19798638-19798657 Msa0012860:CDS 50.0%
CGAGTAGGGATGTTTACCGA+AGG + chr1_1:19798517-19798536 Msa0012860:CDS 50.0%
! TTGACGACGATGATGGCATG+TGG - chr1_1:19798630-19798649 None:intergenic 50.0%
CATCGTCGTCAATGCTGATG+AGG + chr1_1:19798638-19798657 Msa0012860:CDS 50.0%
CAAAGCGCGCTATGCGAGTA+GGG + chr1_1:19798503-19798522 Msa0012860:CDS 55.0%
!!! GATGTCGGCGTATTTTGGCC+TGG + chr1_1:19798736-19798755 Msa0012860:CDS 55.0%
CAAAGCGCGCTATGCGAGTA+GGG + chr1_1:19798503-19798522 Msa0012860:CDS 55.0%
!!! GATGTCGGCGTATTTTGGCC+TGG + chr1_1:19798736-19798755 Msa0012860:CDS 55.0%
GCAAAGCGCGCTATGCGAGT+AGG + chr1_1:19798502-19798521 Msa0012860:CDS 60.0%
GCAAAGCGCGCTATGCGAGT+AGG + chr1_1:19798502-19798521 Msa0012860:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 19798118 19798894 19798118 ID=Msa0012860;Name=Msa0012860
chr1_1 mRNA 19798118 19798894 19798118 ID=Msa0012860-mRNA-1;Parent=Msa0012860;Name=Msa0012860-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|258
chr1_1 exon 19798118 19798894 19798118 ID=Msa0012860-mRNA-1:exon:7105;Parent=Msa0012860-mRNA-1
chr1_1 CDS 19798118 19798894 19798118 ID=Msa0012860-mRNA-1:cds;Parent=Msa0012860-mRNA-1
Gene Sequence

>Msa0012860

ATGATGAATACTCCAAATTTGAACTTGGAAGCTGAAAATAGTTTAGAAGTAAGCAGCCAAGTAGGTTCCAACATATACTTACAAGAATCATCTCATGATCTTACCAAAGACAGTGCCACAACTTCTTCTTGTCTTACTAAGGCAGACATTAATCCAGGTTCTATTTCCCTTGACCTGACTCTCAACTTCAATCCGAGCGATGAAGATTTGAAGGAAACAAGCGACACTAACAGTGAAGTACTAGGAGCTAATCAAGCTCATCATAATACTGTTTCCGCACCAGCGATTCATAGAGTTTTCTCTTGTAATTATTGTAGGAGGAAATTCTTTAGCTCACAAGCGTTAGGTGGACATCAGAATGCTCATAAAAGGGAGAGAACGATGGCAAAGCGCGCTATGCGAGTAGGGATGTTTACCGAAGGGTATACAAGCTTAGCTTCTTTACCTCTTCATGGTTCTTCTTCTTTTCATTCTCTTGGTATTCAAGCTCATGCTGCAATGCACCAAAACCACATGCCATCATCGTCGTCAATGCTGATGAGGGCTCAAGATATGAGACCTACAGCGAAATTCGAGAAAGATTGTTTTGGAGCAGCACCGATGTTTATGCATAATGAAGATGTCGGCGTATTTTGGCCTGGAAGTTTCAGGCAGATAAATCAAAGTGTTCATAATTCAAATTTGAATCATAACTCAAATTTGAATACTAGTTTTGTAGCAACTGAACCTCCTCAACAAACATCTACTACATATCCTGATCTTACTTTGAGGCTTTAA

Protein sequence

>Msa0012860

MMNTPNLNLEAENSLEVSSQVGSNIYLQESSHDLTKDSATTSSCLTKADINPGSISLDLTLNFNPSDEDLKETSDTNSEVLGANQAHHNTVSAPAIHRVFSCNYCRRKFFSSQALGGHQNAHKRERTMAKRAMRVGMFTEGYTSLASLPLHGSSSFHSLGIQAHAAMHQNHMPSSSSMLMRAQDMRPTAKFEKDCFGAAPMFMHNEDVGVFWPGSFRQINQSVHNSNLNHNSNLNTSFVATEPPQQTSTTYPDLTLRL*