AlfalfaGEDB | Msa0013430

M. sativa cultivar zhongmu-4 / Msa0013430

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF12171.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013430	sp\|Q8GYC1\|IDD4_ARATH	79.888	179	27	2	10	183	22	196	4.79e-103	308

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0013430	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013430	MtrunA17_Chr1g0162971	99.454	183	1	0	1	183	1	183	2.62e-138	393
Msa0013430	MtrunA17_Chr1g0163001	84.324	185	27	1	1	183	1	185	1.07e-113	333
Msa0013430	MtrunA17_Chr1g0199101	80.952	189	30	1	1	183	1	189	3.15e-110	325
Msa0013430	MtrunA17_Chr1g0210821	82.258	186	30	1	1	183	1	186	4.21e-106	315
Msa0013430	MtrunA17_Chr7g0263941	86.014	143	20	0	41	183	34	176	8.18e-96	283
Msa0013430	MtrunA17_Chr8g0342421	84.828	145	22	0	39	183	60	204	1.29e-94	284
Msa0013430	MtrunA17_Chr2g0330171	80.519	154	30	0	30	183	35	188	3.64e-94	281
Msa0013430	MtrunA17_Chr1g0181381	78.395	162	32	2	23	183	22	181	6.08e-94	281
Msa0013430	MtrunA17_Chr4g0040571	70.000	180	53	1	5	183	2	181	1.72e-93	280
Msa0013430	MtrunA17_Chr4g0029521	72.571	175	46	2	11	183	7	181	5.26e-93	279

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013430	AT2G02080.1	79.888	179	27	2	10	183	22	196	4.87e-104	308
Msa0013430	AT2G02080.4	79.888	179	27	2	10	183	22	196	4.87e-104	308
Msa0013430	AT2G02080.5	79.888	179	27	2	10	183	22	196	4.87e-104	308
Msa0013430	AT2G02080.3	79.888	179	27	2	10	183	22	196	4.87e-104	308
Msa0013430	AT1G14580.1	82.143	168	27	1	19	183	28	195	8.26e-103	303
Msa0013430	AT1G14580.2	82.143	168	27	1	19	183	28	195	8.26e-103	303
Msa0013430	AT1G14580.3	82.143	168	27	1	19	183	42	209	1.58e-102	303
Msa0013430	AT2G02070.2	91.667	144	12	0	40	183	51	194	3.46e-98	296
Msa0013430	AT2G02070.1	91.667	144	12	0	40	183	51	194	3.46e-98	296
Msa0013430	AT3G50700.1	73.256	172	41	1	12	183	10	176	1.59e-93	280

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Find 32 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTCATTCTAAGACTTG+TGG	0.613886	1_1:-20768181	Msa0013430:CDS
AAGAAAAGGAGAAACCAACC+TGG	0.662135	1_1:-20768868	Msa0013430:CDS
AAGGAGTATAGATGTGACTG+TGG	0.709247	1_1:-20768154	Msa0013430:CDS
AATATGCTGTTCAATCTGAT+TGG	0.427517	1_1:-20768204	Msa0013430:CDS
AATTTACAACTACATAGAAG+AGG	0.622328	1_1:-20768415	Msa0013430:CDS
ACGTATAAGTTGTTATTACC+TGG	0.517908	1_1:+20768120	None:intergenic
ACTATCTCCCAAGAGCCTAA+TGG	0.486645	1_1:-20768491	Msa0013430:CDS
ACTGTGGTACTCTCTTCTCC+AGG	0.561759	1_1:-20768138	Msa0013430:CDS
ACTTGCTTGGTGTTCCAGGT+TGG	0.540376	1_1:+20768854	None:intergenic
AGAAACTTGCTTGGTGTTCC+AGG	0.343228	1_1:+20768850	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTTTACAATCTCACAAG+AGG	-	chr1_1:20768161-20768180	Msa0013430:CDS	30.0%
TGAAAAGCTGTGTCTTTATT+AGG	-	chr1_1:20768489-20768508	Msa0013430:CDS	30.0%
CACAAATAAACCTTTCTCTT+TGG	+	chr1_1:20768777-20768796	None:intergenic	30.0%
AATATGCTGTTCAATCTGAT+TGG	-	chr1_1:20768919-20768938	Msa0013430:CDS	30.0%
TTTCTTTACAATCTCACAAG+AGG	-	chr1_1:20768161-20768180	Msa0013430:CDS	30.0%
TGAAAAGCTGTGTCTTTATT+AGG	-	chr1_1:20768489-20768508	Msa0013430:CDS	30.0%
CACAAATAAACCTTTCTCTT+TGG	+	chr1_1:20768777-20768796	None:intergenic	30.0%
AATATGCTGTTCAATCTGAT+TGG	-	chr1_1:20768919-20768938	Msa0013430:CDS	30.0%
TATGTGAAGTGTGCCAAAAA+GGG	-	chr1_1:20768670-20768689	Msa0013430:intron	35.0%
TAACAAAGAGCCAAAGAGAA+AGG	-	chr1_1:20768764-20768783	Msa0013430:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	20768135	20769010	20768135	ID=Msa0013430;Name=Msa0013430
chr1_1	mRNA	20768135	20769010	20768135	ID=Msa0013430-mRNA-1;Parent=Msa0013430;Name=Msa0013430-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|0\|0\|1\|1\|1\|2\|0\|183
chr1_1	exon	20768860	20769010	20768860	ID=Msa0013430-mRNA-1:exon:7421;Parent=Msa0013430-mRNA-1
chr1_1	exon	20768135	20768535	20768135	ID=Msa0013430-mRNA-1:exon:7420;Parent=Msa0013430-mRNA-1
chr1_1	CDS	20768860	20769010	20768860	ID=Msa0013430-mRNA-1:cds;Parent=Msa0013430-mRNA-1
chr1_1	CDS	20768135	20768535	20768135	ID=Msa0013430-mRNA-1:cds;Parent=Msa0013430-mRNA-1

Gene Sequence

>Msa0013430

ATGGCAGCATCATTTTCAGCTGCATCTTTCTTTACAATCTCACAAGAGGATCAGAATCAGATGAATCAACAGAATTCATCAACACCTTCATCATCAACAACTCCAACTGCACCACCTCAGAAGAAAAGGAGAAACCAACCTGGAACACCAAATCCAGATGCAGAAGTGATAGCACTATCTCCCAAGAGCCTAATGGCAACAAACAGATTTGTATGTGAAGTGTGCCAAAAAGGGTTCCAAAGGGAGCAAAATTTACAACTACATAGAAGAGGACACAATTTGCCTTGGAAGCTAAAGCAAAAGAGTAACAAAGAGCCAAAGAGAAAGGTTTATTTGTGTCCAGAACCAACATGTGTTCACCATGACCCTTCAAGGGCACTTGGTGATCTCACTGGAATTAAGAAACACTATTTTAGAAAGCATGGTGAGAAAAAATGGAAGTGTGAAAAGTGTTCTAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCATTCTAAGACTTGTGGTACTAAGGAGTATAGATGTGACTGTGGTACTCTCTTCTCCAGGTAA

Protein sequence

>Msa0013430

MAASFSAASFFTISQEDQNQMNQQNSSTPSSSTTPTAPPQKKRRNQPGTPNPDAEVIALSPKSLMATNRFVCEVCQKGFQREQNLQLHRRGHNLPWKLKQKSNKEPKRKVYLCPEPTCVHHDPSRALGDLTGIKKHYFRKHGEKKWKCEKCSKKYAVQSDWKAHSKTCGTKEYRCDCGTLFSR*