AlfalfaGEDB | Msa0014450

M. sativa cultivar zhongmu-4 / Msa0014450

KEGG: K11446
GO: GO:0000003 GO:0000902 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008214 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009826 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010605 GO:0010639 GO:0014070 GO:0016043 GO:0016049 GO:0016569 GO:0016570 GO:0016577 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032989 GO:0033043 GO:0033044 GO:0033169 GO:0033993 GO:0035065 GO:0035067 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0099402 GO:1901564 GO:1901700 GO:1901983 GO:1901984 GO:1902275 GO:1905268 GO:2000756 GO:2000757 GO:2001251
PFAM: PF02375.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0014450	sp\|Q336N8\|JM706_ORYSJ	51.496	468	221	4	6	470	101	565	5.91e-165	494

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0014450	TR	Jumonji

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0014450	MtrunA17_Chr1g0164761	88.640	581	47	2	1	581	1	562	0.0	1064
Msa0014450	MtrunA17_Chr1g0164731	78.497	572	96	2	2	572	39	584	0.0	902
Msa0014450	MtrunA17_Chr5g0410721	52.811	498	229	2	2	494	83	579	0.0	561
Msa0014450	MtrunA17_Chr3g0116441	37.778	360	188	9	8	346	18	362	9.50e-67	236
Msa0014450	MtrunA17_Chr8g0380981	37.371	388	168	9	4	325	39	417	2.65e-59	213
Msa0014450	MtrunA17_Chr1g0189211	30.740	527	276	16	6	455	148	662	5.50e-54	198
Msa0014450	MtrunA17_Chr7g0214951	75.833	120	11	2	181	299	99	201	5.72e-54	182
Msa0014450	MtrunA17_Chr5g0397501	35.325	385	204	12	73	431	287	652	3.48e-52	193
Msa0014450	MtrunA17_Chr1g0199511	45.213	188	99	1	152	339	291	474	3.94e-52	193
Msa0014450	MtrunA17_Chr2g0303101	33.170	407	197	9	4	344	48	445	1.61e-51	190

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0014450	AT5G46910.1	53.975	478	212	4	7	480	102	575	0.0	544
Msa0014450	AT5G46910.2	54.565	471	207	3	7	474	102	568	0.0	543
Msa0014450	AT2G34880.1	31.835	534	281	15	7	471	60	579	1.14e-60	215
Msa0014450	AT3G48430.1	34.959	369	196	8	9	339	21	383	1.34e-59	215
Msa0014450	AT1G63490.2	36.000	375	171	10	94	431	306	648	5.39e-56	204
Msa0014450	AT1G63490.4	36.000	375	171	10	94	431	306	648	5.49e-56	204
Msa0014450	AT1G63490.1	36.000	375	171	10	94	431	53	395	5.51e-56	204
Msa0014450	AT4G20400.1	28.440	545	299	15	4	470	52	583	1.90e-55	202
Msa0014450	AT1G30810.3	29.664	536	295	12	4	467	55	580	2.10e-55	201
Msa0014450	AT1G30810.2	29.664	536	295	12	4	467	55	580	2.10e-55	201

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Find 81 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGCAATTTCAATTTGA+AGG	0.380018	1_1:+22894492	Msa0014450:CDS
AAGAAACAATGCGTCTCCAT+CGG	0.561394	1_1:+22896773	Msa0014450:CDS
AAGGTTATGACAAACTCTCC+TGG	0.578383	1_1:-22895290	None:intergenic
AATCCAGCATGATATGAATT+AGG	0.298533	1_1:-22895314	None:intergenic
AATTGAGAGGCTGCAGAACT+AGG	0.476304	1_1:-22895113	None:intergenic
ACACAATTGTACAGCCTCTT+AGG	0.195974	1_1:+22894130	Msa0014450:CDS
ACATGATGTTCCGGTTTACA+AGG	0.469206	1_1:+22895256	Msa0014450:CDS
ACGAGGTCTTACCATGGAAA+AGG	0.511206	1_1:-22896185	None:intergenic
ACTAAGCGCAATGGAGACTG+CGG	0.705785	1_1:-22894722	None:intergenic
ACTCAGGTGCAAATAAAACT+TGG	0.537060	1_1:+22895081	Msa0014450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAAACCCTATAGAAACC+CGG	-	chr1_1:22893697-22893716	None:intergenic	30.0%
TTTGAATTGAAAACCCTAAG+AGG	-	chr1_1:22894147-22894166	None:intergenic	30.0%
GAAATAATACATGGAGAGAA+AGG	+	chr1_1:22894403-22894422	Msa0014450:CDS	30.0%
TTGACAGAAAAATTCCAGTA+AGG	+	chr1_1:22894744-22894763	Msa0014450:intron	30.0%
TAGAAGATCACTATCTGTAT+AGG	+	chr1_1:22894958-22894977	Msa0014450:CDS	30.0%
TCAATTTGAAAAGACTGTCT+TGG	+	chr1_1:22895130-22895149	Msa0014450:CDS	30.0%
AGCATGATATGAATTAGGAA+AGG	-	chr1_1:22895312-22895331	None:intergenic	30.0%
TTTCCTAATTCATATCATGC+TGG	+	chr1_1:22895311-22895330	Msa0014450:CDS	30.0%
AATCCAGCATGATATGAATT+AGG	-	chr1_1:22895317-22895336	None:intergenic	30.0%
CCTTTCTATTATTCAAGCTT+AGG	-	chr1_1:22895482-22895501	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	22893577	22896911	22893577	ID=Msa0014450;Name=Msa0014450
chr1_1	mRNA	22893577	22896911	22893577	ID=Msa0014450-mRNA-1;Parent=Msa0014450;Name=Msa0014450-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|0.12\|0\|1\|1\|1\|9\|0\|581
chr1_1	exon	22893577	22893682	22893577	ID=Msa0014450-mRNA-1:exon:8017;Parent=Msa0014450-mRNA-1
chr1_1	exon	22894046	22894202	22894046	ID=Msa0014450-mRNA-1:exon:8018;Parent=Msa0014450-mRNA-1
chr1_1	exon	22894294	22894513	22894294	ID=Msa0014450-mRNA-1:exon:8019;Parent=Msa0014450-mRNA-1
chr1_1	exon	22894707	22894760	22894707	ID=Msa0014450-mRNA-1:exon:8020;Parent=Msa0014450-mRNA-1
chr1_1	exon	22894900	22894979	22894900	ID=Msa0014450-mRNA-1:exon:8021;Parent=Msa0014450-mRNA-1
chr1_1	exon	22895067	22895344	22895067	ID=Msa0014450-mRNA-1:exon:8022;Parent=Msa0014450-mRNA-1
chr1_1	exon	22895797	22896195	22895797	ID=Msa0014450-mRNA-1:exon:8023;Parent=Msa0014450-mRNA-1
chr1_1	exon	22896290	22896465	22896290	ID=Msa0014450-mRNA-1:exon:8024;Parent=Msa0014450-mRNA-1
chr1_1	exon	22896636	22896911	22896636	ID=Msa0014450-mRNA-1:exon:8025;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22893577	22893682	22893577	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22894046	22894202	22894046	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22894294	22894513	22894294	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22894707	22894760	22894707	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22894900	22894979	22894900	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22895067	22895344	22895067	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22895797	22896195	22895797	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22896290	22896465	22896290	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1	CDS	22896636	22896911	22896636	ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1

Gene Sequence

>Msa0014450

ATGACGAACATTACTCAAGAATGTCCAACTTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCTCCCGAGGCTTCTAAATATGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAAAGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCAATTCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAGAAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCATTTTCCAATAGATTTTGCGGTGAAGAAGATCTCTCTTGTTTAGATATCGAAAAAGTGTTCTGGCACGAAATAATACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATATTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGGCGCGGCTGCCGCAGTCTCCATTGCGCTTAGTTGACAGAAAAATTCCAGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTAGAAGATCACTATCTGTATAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGGATCATGTGTATTGCAACACAGTCTTAAAAGAAAATAGAGAGAATGGTGCATTTCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTACAACATGATGTTCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCATAACCTTTCCTAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTTGGGGCTGCAGCTAGCGAACGTTATTCACGTCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTTACAAAATCAAACCTGAAGATACGGCAACTTATCGAGCTATTGCACTGTCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCGGTCGGAGAGTTCGAGAAAGCTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCAATAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCCGATCCAATTTGCCTTTTCCATGACATAGTGCCACAAAATTGTTTATGTGGGAGAGAACATACTGTATTCAAGAGGAATGACATGTTGGAACTCGAAGATGCTGCCAAAAGTTTTCAACAGGAAAAAGAATGCATCGACGCATTATCTGTAACTAATATATTCTCCTATAACATAAATGAATGCATCCAAGAACACAAGAATAATAACTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCGTTAAGCGCATCATCTAAACTGAAAACAAGAGTTGCGAACAACATCAAGCACACTGTTGAAAATAATCAAAAGAAGACAAAGAATCGACGAAGAAACAATGCGTCTCCATCGGTGATGAGTACCAAAAGACAACGAGTCTTTTATAACTTGAGAAAACATGAATCAGAACTGATTATCGAAGTTCCTGCAGTATCTTCCCTTTTGGCGACATCTGAAAAATCAAAGTAA

Protein sequence

>Msa0014450

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKYGICKIVSPIAASNPASFVLTEEKKDFKFNTIVQPLRVFNSNEMDKITFSKRRRKYTYHEFEALANKAFSNRFCGEEDLSCLDIEKVFWHEIIHGEKGTVEYGVNIEESAFSSDPDDKLGTSNFNLKNLARLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSAASQFEKTVLDHVYCNTVLKENRENGAFQFLAQKTTMFSPDVLLQHDVPVYKAVQKPGEFVITFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASERYSRLKMLPIIPYEELLCKEALLIYNSSKARGYKIKPEDTATYRAIALSFLHLIQFYKTSLSRSESSRKLSSSSNTSLGSVTCSLCNRDCYVAFLLCKKCYSDPICLFHDIVPQNCLCGREHTVFKRNDMLELEDAAKSFQQEKECIDALSVTNIFSYNINECIQEHKNNNWHEEKSARRTVNSLSASSKLKTRVANNIKHTVENNQKKTKNRRRNNASPSVMSTKRQRVFYNLRKHESELIIEVPAVSSLLATSEKSK*