AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0014450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0014450 MtrunA17_Chr1g0164761 88.640 581 47 2 1 581 1 562 0.0 1064
Msa0014450 MtrunA17_Chr1g0164731 78.497 572 96 2 2 572 39 584 0.0 902
Msa0014450 MtrunA17_Chr5g0410721 52.811 498 229 2 2 494 83 579 0.0 561
Msa0014450 MtrunA17_Chr3g0116441 37.778 360 188 9 8 346 18 362 9.50e-67 236
Msa0014450 MtrunA17_Chr8g0380981 37.371 388 168 9 4 325 39 417 2.65e-59 213
Msa0014450 MtrunA17_Chr1g0189211 30.740 527 276 16 6 455 148 662 5.50e-54 198
Msa0014450 MtrunA17_Chr7g0214951 75.833 120 11 2 181 299 99 201 5.72e-54 182
Msa0014450 MtrunA17_Chr5g0397501 35.325 385 204 12 73 431 287 652 3.48e-52 193
Msa0014450 MtrunA17_Chr1g0199511 45.213 188 99 1 152 339 291 474 3.94e-52 193
Msa0014450 MtrunA17_Chr2g0303101 33.170 407 197 9 4 344 48 445 1.61e-51 190
Msa0014450 MtrunA17_Chr7g0261401 31.230 317 191 10 156 456 338 643 8.62e-29 122
Msa0014450 MtrunA17_Chr7g0214941 74.324 74 19 0 469 542 1 74 2.02e-28 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0014450 AT5G46910.1 53.975 478 212 4 7 480 102 575 0.0 544
Msa0014450 AT5G46910.2 54.565 471 207 3 7 474 102 568 0.0 543
Msa0014450 AT2G34880.1 31.835 534 281 15 7 471 60 579 1.14e-60 215
Msa0014450 AT3G48430.1 34.959 369 196 8 9 339 21 383 1.34e-59 215
Msa0014450 AT1G63490.2 36.000 375 171 10 94 431 306 648 5.39e-56 204
Msa0014450 AT1G63490.4 36.000 375 171 10 94 431 306 648 5.49e-56 204
Msa0014450 AT1G63490.1 36.000 375 171 10 94 431 53 395 5.51e-56 204
Msa0014450 AT4G20400.1 28.440 545 299 15 4 470 52 583 1.90e-55 202
Msa0014450 AT1G30810.3 29.664 536 295 12 4 467 55 580 2.10e-55 201
Msa0014450 AT1G30810.2 29.664 536 295 12 4 467 55 580 2.10e-55 201
Msa0014450 AT1G30810.1 29.664 536 295 12 4 467 55 580 2.10e-55 201
Msa0014450 AT5G04240.1 46.809 188 96 1 152 339 259 442 9.03e-53 195
Msa0014450 AT1G08620.3 34.634 410 188 11 6 343 158 559 1.14e-52 194
Msa0014450 AT1G08620.2 34.634 410 188 11 6 343 144 545 1.15e-52 194
Msa0014450 AT1G08620.4 34.634 410 188 11 6 343 144 545 1.15e-52 194
Msa0014450 AT1G08620.1 34.634 410 188 11 6 343 144 545 1.15e-52 194
Msa0014450 AT4G20400.2 28.705 533 289 15 16 470 7 526 2.05e-52 193
Msa0014450 AT2G38950.1 25.158 632 365 21 2 551 102 707 7.90e-36 143
Msa0014450 AT1G63490.3 54.023 87 40 0 254 340 15 101 1.71e-24 109

Find 81 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATTGTACAGCCTCTT+AGG 0.195974 1_1:+22894130 Msa0014450:CDS
TTTCACATACCTTGTGTTCT+TGG 0.229062 1_1:-22896453 None:intergenic
GATGCATTCATTTATGTTAT+AGG 0.249252 1_1:-22896431 None:intergenic
TGTCTTAACGGAAGAGAAAA+AGG 0.267528 1_1:+22894095 Msa0014450:CDS
TTCTTCACCGCAAAATCTAT+TGG 0.284304 1_1:-22894341 None:intergenic
TCCTGCAGTATCTTCCCTTT+TGG 0.292966 1_1:+22896866 Msa0014450:CDS
GCTCCCGAGGCTTCTAAATA+TGG 0.295796 1_1:+22893661 Msa0014450:CDS
AATCCAGCATGATATGAATT+AGG 0.298533 1_1:-22895314 None:intergenic
AGTTCCTCATATGGAATTAT+AGG 0.316333 1_1:-22895892 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1_1:+22894418 Msa0014450:CDS
ATAAAAGACTCGTTGTCTTT+TGG 0.328314 1_1:-22896805 None:intergenic
CGATAAGTTGCCGTATCTTC+AGG 0.338048 1_1:-22895976 None:intergenic
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_1:-22895901 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1_1:-22896177 None:intergenic
AGCTCTTCAAATACATCATT+AGG 0.370354 1_1:+22896081 Msa0014450:CDS
TGACATGCAGCCTCTGCAAT+AGG 0.375602 1_1:+22896109 Msa0014450:CDS
AACAAGCAATTTCAATTTGA+AGG 0.380018 1_1:+22894492 Msa0014450:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_1:+22895818 Msa0014450:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1_1:-22894076 None:intergenic
CTTGTTACAACATGATGTTC+CGG 0.394142 1_1:+22895247 Msa0014450:CDS
CTTGAAAGCAGAATAATAAC+TGG 0.406912 1_1:+22896625 Msa0014450:intron
TTGGTGATTGGTTTCCACTT+GGG 0.409363 1_1:+22895830 Msa0014450:CDS
TAGAAGATCACTATCTGTAT+AGG 0.412721 1_1:+22894958 Msa0014450:CDS
CTGATCCTATGCTTTACATT+GGG 0.424948 1_1:+22894907 Msa0014450:CDS
TCAATTTGAAAAGACTGTCT+TGG 0.425142 1_1:+22895130 Msa0014450:CDS
TGTTCTGATGGATAATAAGT+TGG 0.426588 1_1:-22893601 None:intergenic
TTTGAATTGAAAACCCTAAG+AGG 0.432607 1_1:-22894144 None:intergenic
CACAATTGTACAGCCTCTTA+GGG 0.435355 1_1:+22894131 Msa0014450:CDS
GTGCCACAAAATTGTTTATG+TGG 0.436841 1_1:+22896295 Msa0014450:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1_1:-22894475 None:intergenic
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_1:+22895811 Msa0014450:CDS
GCTACCTATAATTCCATATG+AGG 0.458631 1_1:+22895888 Msa0014450:CDS
TGGTGATTGGTTTCCACTTG+GGG 0.467102 1_1:+22895831 Msa0014450:CDS
ACATGATGTTCCGGTTTACA+AGG 0.469206 1_1:+22895256 Msa0014450:CDS
AATTGAGAGGCTGCAGAACT+AGG 0.476304 1_1:-22895113 None:intergenic
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1_1:-22893613 None:intergenic
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1_1:+22895272 Msa0014450:CDS
TTTCCTAATTCATATCATGC+TGG 0.481919 1_1:+22895311 Msa0014450:CDS
ATTGGTGATTGGTTTCCACT+TGG 0.491227 1_1:+22895829 Msa0014450:CDS
ATTTGAAGCTCTTGCAAACA+AGG 0.496702 1_1:+22894312 Msa0014450:CDS
TTAAAAGAAAATAGAGAGAA+TGG 0.497403 1_1:+22895176 Msa0014450:CDS
GACATGCAGCCTCTGCAATA+GGG 0.498995 1_1:+22896110 Msa0014450:CDS
ATCATCAGGATCACTTGAAA+AGG 0.501772 1_1:-22894461 None:intergenic
GAAATAATACATGGAGAGAA+AGG 0.501914 1_1:+22894403 Msa0014450:CDS
GGTGCAAATAAAACTTGGTA+TGG 0.503293 1_1:+22895086 Msa0014450:CDS
ACGAGGTCTTACCATGGAAA+AGG 0.511206 1_1:-22896185 None:intergenic
CAAAATCAAACCTGAAGATA+CGG 0.514275 1_1:+22895966 Msa0014450:CDS
ACTGATCCTATGCTTTACAT+TGG 0.515442 1_1:+22894906 Msa0014450:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1_1:+22894469 Msa0014450:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_1:+22894931 Msa0014450:CDS
TCATGTTGTAACAAGACATC+AGG 0.521145 1_1:-22895239 None:intergenic
TCCGTTAAGACAAAAGAAGC+AGG 0.523048 1_1:-22894084 None:intergenic
ACTCAGGTGCAAATAAAACT+TGG 0.537060 1_1:+22895081 Msa0014450:CDS
TGCCACAAAATTGTTTATGT+GGG 0.552294 1_1:+22896296 Msa0014450:CDS
ATACCATATTTAGAAGCCTC+GGG 0.558433 1_1:-22893664 None:intergenic
CATACCATATTTAGAAGCCT+CGG 0.559311 1_1:-22893665 None:intergenic
GCAGGATTAGAGGCAGCGAT+TGG 0.560174 1_1:-22894066 None:intergenic
AAGAAACAATGCGTCTCCAT+CGG 0.561394 1_1:+22896773 Msa0014450:CDS
CAAGACATCAGGAGAGAACA+TGG 0.563327 1_1:-22895228 None:intergenic
TAAGACATCGCTATCGCGGT+CGG 0.566148 1_1:+22896038 Msa0014450:CDS
TTTATAAGACATCGCTATCG+CGG 0.569010 1_1:+22896034 Msa0014450:CDS
TTTCTGTCAACTAAGCGCAA+TGG 0.570360 1_1:-22894731 None:intergenic
TCGCTAGCTGCAGCCCCAAG+TGG 0.576234 1_1:-22895844 None:intergenic
AAGGTTATGACAAACTCTCC+TGG 0.578383 1_1:-22895290 None:intergenic
ATTCAAGAGGAATGACATGT+TGG 0.584294 1_1:+22896333 Msa0014450:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1_1:+22895323 Msa0014450:CDS
TTCTGGCACGAAATAATACA+TGG 0.588933 1_1:+22894394 Msa0014450:CDS
GCCAAAAGGGAAGATACTGC+AGG 0.591239 1_1:-22896867 None:intergenic
ATGGAAAAGGCAAATTGGAT+CGG 0.598986 1_1:-22896172 None:intergenic
TCTGCAGAAAATTGCTCCCG+AGG 0.609917 1_1:+22893648 Msa0014450:CDS
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_1:-22894912 None:intergenic
TACAACTCCTCAAAAGCTAG+AGG 0.624733 1_1:+22895940 Msa0014450:CDS
AGCATAAATTACCATCACTC+AGG 0.628951 1_1:+22895065 Msa0014450:intron
GAGAACATACTGTATTCAAG+AGG 0.632379 1_1:+22896320 Msa0014450:CDS
AGCATGATATGAATTAGGAA+AGG 0.639278 1_1:-22895309 None:intergenic
GCAGAATAATAACTGGCATG+AGG 0.639560 1_1:+22896632 Msa0014450:intron
CATAACAATCCCTATTGCAG+AGG 0.648673 1_1:-22896119 None:intergenic
TGAATGCATCCAAGAACACA+AGG 0.658928 1_1:+22896444 Msa0014450:CDS
TGAGTATGGAGTCAATATTG+AGG 0.665538 1_1:+22894432 Msa0014450:CDS
TGATGTTCCGGTTTACAAGG+CGG 0.676766 1_1:+22895259 Msa0014450:CDS
ACTAAGCGCAATGGAGACTG+CGG 0.705785 1_1:-22894722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTGATTTTAGAATT+TGG + chr1_1:22894691-22894710 Msa0014450:intron 10.0%
!! AGAACAATGTAATATAATAT+AGG + chr1_1:22893896-22893915 Msa0014450:intron 15.0%
!! GAACAATGTAATATAATATA+GGG + chr1_1:22893897-22893916 Msa0014450:intron 15.0%
!!! TTTTTTTGACAAAGTAATAT+AGG + chr1_1:22893934-22893953 Msa0014450:intron 15.0%
!!! TTTTTTGACAAAGTAATATA+GGG + chr1_1:22893935-22893954 Msa0014450:intron 15.0%
!! ATATTTGATCTTAATATTTC+AGG + chr1_1:22894024-22894043 Msa0014450:intron 15.0%
!! TAAATATGGTATGTTATTAC+CGG + chr1_1:22893675-22893694 Msa0014450:intron 20.0%
!!! TTTTAGCAAAACATATACTT+TGG - chr1_1:22893851-22893870 None:intergenic 20.0%
!!! TATATGTTTTGCTAAAATCA+TGG + chr1_1:22893854-22893873 Msa0014450:intron 20.0%
!! TGATGAAAAATTAACTAACT+TGG + chr1_1:22894249-22894268 Msa0014450:intron 20.0%
!!! AACAACTTTTTATTCAGATT+AGG - chr1_1:22894785-22894804 None:intergenic 20.0%
!! TTAAAAGAAAATAGAGAGAA+TGG + chr1_1:22895176-22895195 Msa0014450:CDS 20.0%
!! ATTCTGCTAGATATTATTAT+GGG - chr1_1:22895662-22895681 None:intergenic 20.0%
!! AATTCTGCTAGATATTATTA+TGG - chr1_1:22895663-22895682 None:intergenic 20.0%
!!! CATAAGTATTTAAAACAGAA+GGG - chr1_1:22895687-22895706 None:intergenic 20.0%
!!! ACATAAGTATTTAAAACAGA+AGG - chr1_1:22895688-22895707 None:intergenic 20.0%
!! ATTTTCTGCAGATAAACTAA+AGG - chr1_1:22893640-22893659 None:intergenic 25.0%
! AAATATGGTATGTTATTACC+GGG + chr1_1:22893676-22893695 Msa0014450:intron 25.0%
!!! TAACTTTTTCTAAGAGAAGA+AGG + chr1_1:22894181-22894200 Msa0014450:CDS 25.0%
! AACAAGCAATTTCAATTTGA+AGG + chr1_1:22894492-22894511 Msa0014450:CDS 25.0%
!! TACAAAAATTGATTCCTTAC+TGG - chr1_1:22894761-22894780 None:intergenic 25.0%
! CTTTCTATTATTCAAGCTTA+GGG - chr1_1:22895481-22895500 None:intergenic 25.0%
!!! AATCTGTTTATTTTTGTACC+AGG + chr1_1:22895574-22895593 Msa0014450:intron 25.0%
!! ATCATTTTCTTGTTGAGTTT+AGG + chr1_1:22895775-22895794 Msa0014450:intron 25.0%
! GCAACTTAAATAACAATTTC+TGG - chr1_1:22896226-22896245 None:intergenic 25.0%
!!! ATGGATAAACTTGCTAATTA+TGG + chr1_1:22896257-22896276 Msa0014450:intron 25.0%
!! TTTTTCCTGTTGAAAACTTT+TGG - chr1_1:22896374-22896393 None:intergenic 25.0%
! GATGCATTCATTTATGTTAT+AGG - chr1_1:22896434-22896453 None:intergenic 25.0%
!!! ACTATTTTGATGAAGTTTAG+AGG - chr1_1:22896513-22896532 None:intergenic 25.0%
! TGTTCTGATGGATAATAAGT+TGG - chr1_1:22893604-22893623 None:intergenic 30.0%
AAATAAACCCTATAGAAACC+CGG - chr1_1:22893697-22893716 None:intergenic 30.0%
!! GAATGCTACTTACTTTCTTT+TGG + chr1_1:22893744-22893763 Msa0014450:intron 30.0%
! ATGTTTTGCTAAAATCATGG+TGG + chr1_1:22893857-22893876 Msa0014450:intron 30.0%
TTTGAATTGAAAACCCTAAG+AGG - chr1_1:22894147-22894166 None:intergenic 30.0%
! GGTTTTCAATTCAAATGAGA+TGG + chr1_1:22894152-22894171 Msa0014450:CDS 30.0%
! GCATTTTCCAATAGATTTTG+CGG + chr1_1:22894334-22894353 Msa0014450:CDS 30.0%
!! TAGATATCGAAAAAGTGTTC+TGG + chr1_1:22894377-22894396 Msa0014450:CDS 30.0%
GAAATAATACATGGAGAGAA+AGG + chr1_1:22894403-22894422 Msa0014450:CDS 30.0%
!!! TTTGATTTTAGAATTTGGCG+CGG + chr1_1:22894696-22894715 Msa0014450:intron 30.0%
TTGACAGAAAAATTCCAGTA+AGG + chr1_1:22894744-22894763 Msa0014450:intron 30.0%
TAGAAGATCACTATCTGTAT+AGG + chr1_1:22894958-22894977 Msa0014450:CDS 30.0%
TCAATTTGAAAAGACTGTCT+TGG + chr1_1:22895130-22895149 Msa0014450:CDS 30.0%
AGCATGATATGAATTAGGAA+AGG - chr1_1:22895312-22895331 None:intergenic 30.0%
TTTCCTAATTCATATCATGC+TGG + chr1_1:22895311-22895330 Msa0014450:CDS 30.0%
AATCCAGCATGATATGAATT+AGG - chr1_1:22895317-22895336 None:intergenic 30.0%
CCTTTCTATTATTCAAGCTT+AGG - chr1_1:22895482-22895501 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG + chr1_1:22895479-22895498 Msa0014450:intron 30.0%
CGAAATTTGATTATGGAGTT+CGG - chr1_1:22895542-22895561 None:intergenic 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG + chr1_1:22895787-22895806 Msa0014450:intron 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + chr1_1:22895818-22895837 Msa0014450:CDS 30.0%
AGTTCCTCATATGGAATTAT+AGG - chr1_1:22895895-22895914 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG - chr1_1:22895904-22895923 None:intergenic 30.0%
CAAAATCAAACCTGAAGATA+CGG + chr1_1:22895966-22895985 Msa0014450:CDS 30.0%
AGCTCTTCAAATACATCATT+AGG + chr1_1:22896081-22896100 Msa0014450:CDS 30.0%
! TAAGTTGCTTGTGTTATTGA+TGG + chr1_1:22896238-22896257 Msa0014450:intron 30.0%
TGCCACAAAATTGTTTATGT+GGG + chr1_1:22896296-22896315 Msa0014450:CDS 30.0%
CTTGAAAGCAGAATAATAAC+TGG + chr1_1:22896625-22896644 Msa0014450:intron 30.0%
! ATAAAAGACTCGTTGTCTTT+TGG - chr1_1:22896808-22896827 None:intergenic 30.0%
ATACCATATTTAGAAGCCTC+GGG - chr1_1:22893667-22893686 None:intergenic 35.0%
CATACCATATTTAGAAGCCT+CGG - chr1_1:22893668-22893687 None:intergenic 35.0%
TGTTATTACCGGGTTTCTAT+AGG + chr1_1:22893686-22893705 Msa0014450:intron 35.0%
GTTATTACCGGGTTTCTATA+GGG + chr1_1:22893687-22893706 Msa0014450:intron 35.0%
CGTGTGCATGTTTAGATTAA+CGG + chr1_1:22893791-22893810 Msa0014450:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+CGG + chr1_1:22894083-22894102 Msa0014450:CDS 35.0%
TGTCTTAACGGAAGAGAAAA+AGG + chr1_1:22894095-22894114 Msa0014450:CDS 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG + chr1_1:22894312-22894331 Msa0014450:CDS 35.0%
TTCTTCACCGCAAAATCTAT+TGG - chr1_1:22894344-22894363 None:intergenic 35.0%
TTCTGGCACGAAATAATACA+TGG + chr1_1:22894394-22894413 Msa0014450:CDS 35.0%
TGAGTATGGAGTCAATATTG+AGG + chr1_1:22894432-22894451 Msa0014450:CDS 35.0%
ATCATCAGGATCACTTGAAA+AGG - chr1_1:22894464-22894483 None:intergenic 35.0%
ACTGATCCTATGCTTTACAT+TGG + chr1_1:22894906-22894925 Msa0014450:CDS 35.0%
CTGATCCTATGCTTTACATT+GGG + chr1_1:22894907-22894926 Msa0014450:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + chr1_1:22894931-22894950 Msa0014450:CDS 35.0%
AGCATAAATTACCATCACTC+AGG + chr1_1:22895065-22895084 Msa0014450:intron 35.0%
!! TTTTATTTGCACCTGAGTGA+TGG - chr1_1:22895079-22895098 None:intergenic 35.0%
ACTCAGGTGCAAATAAAACT+TGG + chr1_1:22895081-22895100 Msa0014450:CDS 35.0%
GGTGCAAATAAAACTTGGTA+TGG + chr1_1:22895086-22895105 Msa0014450:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - chr1_1:22895129-22895148 None:intergenic 35.0%
TCATGTTGTAACAAGACATC+AGG - chr1_1:22895242-22895261 None:intergenic 35.0%
CTTGTTACAACATGATGTTC+CGG + chr1_1:22895247-22895266 Msa0014450:CDS 35.0%
TATCATGCTGGATTTAGTCA+TGG + chr1_1:22895323-22895342 Msa0014450:CDS 35.0%
CTTGTGTAAATAGAGAGAGA+GGG - chr1_1:22895447-22895466 None:intergenic 35.0%
ACTTGTGTAAATAGAGAGAG+AGG - chr1_1:22895448-22895467 None:intergenic 35.0%
TATTCAAGCTTAGGGTTTAC+TGG - chr1_1:22895473-22895492 None:intergenic 35.0%
GAACACGCGAAATTTGATTA+TGG - chr1_1:22895549-22895568 None:intergenic 35.0%
AACTATTAGAACTTCAAGCC+TGG - chr1_1:22895595-22895614 None:intergenic 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + chr1_1:22895811-22895830 Msa0014450:CDS 35.0%
GCTACCTATAATTCCATATG+AGG + chr1_1:22895888-22895907 Msa0014450:CDS 35.0%
!!! TTTGTAACCTCTAGCTTTTG+AGG - chr1_1:22895950-22895969 None:intergenic 35.0%
TTTATAAGACATCGCTATCG+CGG + chr1_1:22896034-22896053 Msa0014450:CDS 35.0%
ATGGAAAAGGCAAATTGGAT+CGG - chr1_1:22896175-22896194 None:intergenic 35.0%
TTACCATGGAAAAGGCAAAT+TGG - chr1_1:22896180-22896199 None:intergenic 35.0%
GTGCCACAAAATTGTTTATG+TGG + chr1_1:22896295-22896314 Msa0014450:CDS 35.0%
! CTCCCACATAAACAATTTTG+TGG - chr1_1:22896301-22896320 None:intergenic 35.0%
GAGAACATACTGTATTCAAG+AGG + chr1_1:22896320-22896339 Msa0014450:CDS 35.0%
ATTCAAGAGGAATGACATGT+TGG + chr1_1:22896333-22896352 Msa0014450:CDS 35.0%
TTTCACATACCTTGTGTTCT+TGG - chr1_1:22896456-22896475 None:intergenic 35.0%
GTATGCATTGCTTTATCTTC+AGG + chr1_1:22896556-22896575 Msa0014450:intron 35.0%
!! TTTTTCAGATGTCGCCAAAA+GGG - chr1_1:22896883-22896902 None:intergenic 35.0%
!! ATTTTTCAGATGTCGCCAAA+AGG - chr1_1:22896884-22896903 None:intergenic 35.0%
TGCTCAAACTCTTGTTCTGA+TGG - chr1_1:22893616-22893635 None:intergenic 40.0%
GACAAAAGAAGCAGGATTAG+AGG - chr1_1:22894079-22894098 None:intergenic 40.0%
TCCGTTAAGACAAAAGAAGC+AGG - chr1_1:22894087-22894106 None:intergenic 40.0%
ACACAATTGTACAGCCTCTT+AGG + chr1_1:22894130-22894149 Msa0014450:CDS 40.0%
CACAATTGTACAGCCTCTTA+GGG + chr1_1:22894131-22894150 Msa0014450:CDS 40.0%
GAGAAAGGAACAGTTGAGTA+TGG + chr1_1:22894418-22894437 Msa0014450:CDS 40.0%
AGTGATCCTGATGATAAGCT+TGG + chr1_1:22894469-22894488 Msa0014450:CDS 40.0%
CTTGTTCCAAGCTTATCATC+AGG - chr1_1:22894478-22894497 None:intergenic 40.0%
TTTCTGTCAACTAAGCGCAA+TGG - chr1_1:22894734-22894753 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG - chr1_1:22894915-22894934 None:intergenic 40.0%
ACATGATGTTCCGGTTTACA+AGG + chr1_1:22895256-22895275 Msa0014450:CDS 40.0%
AAGGTTATGACAAACTCTCC+TGG - chr1_1:22895293-22895312 None:intergenic 40.0%
TAAATAGAGAGAGAGGGAGA+GGG - chr1_1:22895441-22895460 None:intergenic 40.0%
!! ATTGGTGATTGGTTTCCACT+TGG + chr1_1:22895829-22895848 Msa0014450:CDS 40.0%
!! TTGGTGATTGGTTTCCACTT+GGG + chr1_1:22895830-22895849 Msa0014450:CDS 40.0%
TACAACTCCTCAAAAGCTAG+AGG + chr1_1:22895940-22895959 Msa0014450:CDS 40.0%
CATAACAATCCCTATTGCAG+AGG - chr1_1:22896122-22896141 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG + chr1_1:22896174-22896193 Msa0014450:CDS 40.0%
AGAATAACGAGGTCTTACCA+TGG - chr1_1:22896194-22896213 None:intergenic 40.0%
GGAATACTCAGAGAATAACG+AGG - chr1_1:22896205-22896224 None:intergenic 40.0%
! TGCTGCCAAAAGTTTTCAAC+AGG + chr1_1:22896366-22896385 Msa0014450:CDS 40.0%
TGAATGCATCCAAGAACACA+AGG + chr1_1:22896444-22896463 Msa0014450:CDS 40.0%
GCAGAATAATAACTGGCATG+AGG + chr1_1:22896632-22896651 Msa0014450:intron 40.0%
AAGAAACAATGCGTCTCCAT+CGG + chr1_1:22896773-22896792 Msa0014450:CDS 40.0%
GAACTTGAGTACTTCAGCGA+TGG - chr1_1:22893772-22893791 None:intergenic 45.0%
AATTGAGAGGCTGCAGAACT+AGG - chr1_1:22895116-22895135 None:intergenic 45.0%
CAAGACATCAGGAGAGAACA+TGG - chr1_1:22895231-22895250 None:intergenic 45.0%
TGATGTTCCGGTTTACAAGG+CGG + chr1_1:22895259-22895278 Msa0014450:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+CGG - chr1_1:22895269-22895288 None:intergenic 45.0%
GTAAATAGAGAGAGAGGGAG+AGG - chr1_1:22895442-22895461 None:intergenic 45.0%
GTATTTCCACCACTATGCAG+CGG + chr1_1:22895501-22895520 Msa0014450:intron 45.0%
!! TGGTGATTGGTTTCCACTTG+GGG + chr1_1:22895831-22895850 Msa0014450:CDS 45.0%
CGATAAGTTGCCGTATCTTC+AGG - chr1_1:22895979-22895998 None:intergenic 45.0%
ACGAGGTCTTACCATGGAAA+AGG - chr1_1:22896188-22896207 None:intergenic 45.0%
! CTTTTGGTACTCATCACCGA+TGG - chr1_1:22896792-22896811 None:intergenic 45.0%
! TCCTGCAGTATCTTCCCTTT+TGG + chr1_1:22896866-22896885 Msa0014450:CDS 45.0%
!!! TTAATTTTATTTACAATTTT+AGG + chr1_1:22894878-22894897 Msa0014450:intron 5.0%
!!! TAATTTTATTTACAATTTTA+GGG + chr1_1:22894879-22894898 Msa0014450:intron 5.0%
TCTGCAGAAAATTGCTCCCG+AGG + chr1_1:22893648-22893667 Msa0014450:CDS 50.0%
GCTCCCGAGGCTTCTAAATA+TGG + chr1_1:22893661-22893680 Msa0014450:CDS 50.0%
ACTAAGCGCAATGGAGACTG+CGG - chr1_1:22894725-22894744 None:intergenic 50.0%
TGCAGCGGCATAGGAACAAT+AGG + chr1_1:22895516-22895535 Msa0014450:intron 50.0%
TAAGACATCGCTATCGCGGT+CGG + chr1_1:22896038-22896057 Msa0014450:CDS 50.0%
TGACATGCAGCCTCTGCAAT+AGG + chr1_1:22896109-22896128 Msa0014450:CDS 50.0%
GACATGCAGCCTCTGCAATA+GGG + chr1_1:22896110-22896129 Msa0014450:CDS 50.0%
GCCAAAAGGGAAGATACTGC+AGG - chr1_1:22896870-22896889 None:intergenic 50.0%
GCAGGATTAGAGGCAGCGAT+TGG - chr1_1:22894069-22894088 None:intergenic 55.0%
TACAAGGCGGTGCAAAAGCC+AGG + chr1_1:22895272-22895291 Msa0014450:CDS 55.0%
GTTCCTATGCCGCTGCATAG+TGG - chr1_1:22895513-22895532 None:intergenic 55.0%
GAGAGAGAGGGAGAGGGAGA+GGG - chr1_1:22895435-22895454 None:intergenic 60.0%
AGAGAGAGAGGGAGAGGGAG+AGG - chr1_1:22895436-22895455 None:intergenic 60.0%
CCTATGCCGCTGCATAGTGG+TGG - chr1_1:22895510-22895529 None:intergenic 60.0%
CCACCACTATGCAGCGGCAT+AGG + chr1_1:22895507-22895526 Msa0014450:intron 60.0%
AGAGGGAGAGGGAGGGGGAA+GGG - chr1_1:22895418-22895437 None:intergenic 65.0%
AGAGGGAGAGGGAGAGGGAG+GGG - chr1_1:22895424-22895443 None:intergenic 65.0%
GAGAGGGAGAGGGAGAGGGA+GGG - chr1_1:22895425-22895444 None:intergenic 65.0%
GAGGGAGAGGGAGAGGGAGA+GGG - chr1_1:22895429-22895448 None:intergenic 65.0%
AGAGGGAGAGGGAGAGGGAG+AGG - chr1_1:22895424-22895443 None:intergenic 65.0%
TCGCTAGCTGCAGCCCCAAG+TGG - chr1_1:22895847-22895866 None:intergenic 65.0%
GGGAGGGAAGGAGGGAGGGA+GGG - chr1_1:22895371-22895390 None:intergenic 70.0%
AGGGAGGGAAGGAGGGAGGG+AGG - chr1_1:22895372-22895391 None:intergenic 70.0%
GGGAGGGAGGGAAGGAGGGA+GGG - chr1_1:22895375-22895394 None:intergenic 70.0%
AGGGAGGGAGGGAAGGAGGG+AGG - chr1_1:22895376-22895395 None:intergenic 70.0%
GGGAGGGAGGGAGGGAAGGA+GGG - chr1_1:22895379-22895398 None:intergenic 70.0%
AGGGAGGGAGGGAGGGAAGG+AGG - chr1_1:22895380-22895399 None:intergenic 70.0%
AGAGGGAGGGGGAAGGGGAG+GGG - chr1_1:22895412-22895431 None:intergenic 70.0%
GAGAGGGAGGGGGAAGGGGA+GGG - chr1_1:22895413-22895432 None:intergenic 70.0%
GAGGGAGAGGGAGGGGGAAG+GGG - chr1_1:22895417-22895436 None:intergenic 70.0%
GAGAGGGAGAGGGAGGGGGA+AGG - chr1_1:22895419-22895438 None:intergenic 70.0%
GAGGGAGAGGGAGAGGGAGG+GGG - chr1_1:22895423-22895442 None:intergenic 70.0%
GGAGAGGGAGAGGGAGAGGG+AGG - chr1_1:22895426-22895445 None:intergenic 70.0%
GGGAGGGAGGGAGGGAGGGA+AGG - chr1_1:22895383-22895402 None:intergenic 75.0%
GGGAGGGAGGGAGGGAGGGA+GGG - chr1_1:22895383-22895402 None:intergenic 75.0%
AGGGAGGGAGGGAGGGAGGG+AGG - chr1_1:22895384-22895403 None:intergenic 75.0%
GGGAGGGAGGGAGGGAGGGA+GGG - chr1_1:22895383-22895402 None:intergenic 75.0%
GAGGGGGAGGGAGGGAGGGA+GGG - chr1_1:22895395-22895414 None:intergenic 75.0%
AGGGGAGGGGGAGGGAGGGA+GGG - chr1_1:22895399-22895418 None:intergenic 75.0%
AAGGGGAGGGGGAGGGAGGG+AGG - chr1_1:22895400-22895419 None:intergenic 75.0%
GGGAAGGGGAGGGGGAGGGA+GGG - chr1_1:22895403-22895422 None:intergenic 75.0%
GAGGGGGAAGGGGAGGGGGA+GGG - chr1_1:22895407-22895426 None:intergenic 75.0%
GAGGGAGGGGGAAGGGGAGG+GGG - chr1_1:22895411-22895430 None:intergenic 75.0%
GGAGAGGGAGGGGGAAGGGG+AGG - chr1_1:22895414-22895433 None:intergenic 75.0%
! GGGGAGGGAGGGAGGGAGGG+AGG - chr1_1:22895392-22895411 None:intergenic 80.0%
! GGAGGGGGAGGGAGGGAGGG+AGG - chr1_1:22895396-22895415 None:intergenic 80.0%
! GGGGAAGGGGAGGGGGAGGG+AGG - chr1_1:22895404-22895423 None:intergenic 80.0%
! GGAGGGGGAAGGGGAGGGGG+AGG - chr1_1:22895408-22895427 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 22893577 22896911 22893577 ID=Msa0014450;Name=Msa0014450
chr1_1 mRNA 22893577 22896911 22893577 ID=Msa0014450-mRNA-1;Parent=Msa0014450;Name=Msa0014450-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|0.12|0|1|1|1|9|0|581
chr1_1 exon 22893577 22893682 22893577 ID=Msa0014450-mRNA-1:exon:8017;Parent=Msa0014450-mRNA-1
chr1_1 exon 22894046 22894202 22894046 ID=Msa0014450-mRNA-1:exon:8018;Parent=Msa0014450-mRNA-1
chr1_1 exon 22894294 22894513 22894294 ID=Msa0014450-mRNA-1:exon:8019;Parent=Msa0014450-mRNA-1
chr1_1 exon 22894707 22894760 22894707 ID=Msa0014450-mRNA-1:exon:8020;Parent=Msa0014450-mRNA-1
chr1_1 exon 22894900 22894979 22894900 ID=Msa0014450-mRNA-1:exon:8021;Parent=Msa0014450-mRNA-1
chr1_1 exon 22895067 22895344 22895067 ID=Msa0014450-mRNA-1:exon:8022;Parent=Msa0014450-mRNA-1
chr1_1 exon 22895797 22896195 22895797 ID=Msa0014450-mRNA-1:exon:8023;Parent=Msa0014450-mRNA-1
chr1_1 exon 22896290 22896465 22896290 ID=Msa0014450-mRNA-1:exon:8024;Parent=Msa0014450-mRNA-1
chr1_1 exon 22896636 22896911 22896636 ID=Msa0014450-mRNA-1:exon:8025;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22893577 22893682 22893577 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22894046 22894202 22894046 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22894294 22894513 22894294 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22894707 22894760 22894707 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22894900 22894979 22894900 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22895067 22895344 22895067 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22895797 22896195 22895797 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22896290 22896465 22896290 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
chr1_1 CDS 22896636 22896911 22896636 ID=Msa0014450-mRNA-1:cds;Parent=Msa0014450-mRNA-1
Gene Sequence

>Msa0014450

ATGACGAACATTACTCAAGAATGTCCAACTTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCTCCCGAGGCTTCTAAATATGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAAAGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCAATTCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAGAAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCATTTTCCAATAGATTTTGCGGTGAAGAAGATCTCTCTTGTTTAGATATCGAAAAAGTGTTCTGGCACGAAATAATACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATATTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGGCGCGGCTGCCGCAGTCTCCATTGCGCTTAGTTGACAGAAAAATTCCAGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTAGAAGATCACTATCTGTATAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGGATCATGTGTATTGCAACACAGTCTTAAAAGAAAATAGAGAGAATGGTGCATTTCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTACAACATGATGTTCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCATAACCTTTCCTAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTTGGGGCTGCAGCTAGCGAACGTTATTCACGTCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTTACAAAATCAAACCTGAAGATACGGCAACTTATCGAGCTATTGCACTGTCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCGGTCGGAGAGTTCGAGAAAGCTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCAATAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCCGATCCAATTTGCCTTTTCCATGACATAGTGCCACAAAATTGTTTATGTGGGAGAGAACATACTGTATTCAAGAGGAATGACATGTTGGAACTCGAAGATGCTGCCAAAAGTTTTCAACAGGAAAAAGAATGCATCGACGCATTATCTGTAACTAATATATTCTCCTATAACATAAATGAATGCATCCAAGAACACAAGAATAATAACTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCGTTAAGCGCATCATCTAAACTGAAAACAAGAGTTGCGAACAACATCAAGCACACTGTTGAAAATAATCAAAAGAAGACAAAGAATCGACGAAGAAACAATGCGTCTCCATCGGTGATGAGTACCAAAAGACAACGAGTCTTTTATAACTTGAGAAAACATGAATCAGAACTGATTATCGAAGTTCCTGCAGTATCTTCCCTTTTGGCGACATCTGAAAAATCAAAGTAA
Protein sequence

>Msa0014450

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKYGICKIVSPIAASNPASFVLTEEKKDFKFNTIVQPLRVFNSNEMDKITFSKRRRKYTYHEFEALANKAFSNRFCGEEDLSCLDIEKVFWHEIIHGEKGTVEYGVNIEESAFSSDPDDKLGTSNFNLKNLARLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSAASQFEKTVLDHVYCNTVLKENRENGAFQFLAQKTTMFSPDVLLQHDVPVYKAVQKPGEFVITFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASERYSRLKMLPIIPYEELLCKEALLIYNSSKARGYKIKPEDTATYRAIALSFLHLIQFYKTSLSRSESSRKLSSSSNTSLGSVTCSLCNRDCYVAFLLCKKCYSDPICLFHDIVPQNCLCGREHTVFKRNDMLELEDAAKSFQQEKECIDALSVTNIFSYNINECIQEHKNNNWHEEKSARRTVNSLSASSKLKTRVANNIKHTVENNQKKTKNRRRNNASPSVMSTKRQRVFYNLRKHESELIIEVPAVSSLLATSEKSK*