AlfalfaGEDB | Msa0015900

M. sativa cultivar zhongmu-4 / Msa0015900

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0015900	sp\|Q7XBH4\|MYB4_ORYSJ	41.818	275	97	7	1	230	1	257	1.30e-57	186

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0015900	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0015900	MtrunA17_Chr1g0167661	73.462	260	33	2	1	230	1	254	8.44e-135	379
Msa0015900	MtrunA17_Chr1g0167691	76.628	261	24	4	1	230	1	255	5.52e-129	364
Msa0015900	MtrunA17_Chr1g0187761	55.094	265	77	6	1	230	1	258	6.63e-84	250
Msa0015900	MtrunA17_Chr7g0261281	40.702	285	107	7	1	230	1	278	1.60e-53	173
Msa0015900	MtrunA17_Chr7g0274581	35.798	257	79	7	1	223	1	205	3.71e-43	144
Msa0015900	MtrunA17_Chr1g0203411	59.483	116	17	1	1	86	1	116	4.55e-42	144
Msa0015900	MtrunA17_Chr2g0323351	52.318	151	32	4	1	120	1	142	2.07e-40	139
Msa0015900	MtrunA17_Chr4g0039971	58.120	117	19	1	1	87	2	118	1.03e-39	139
Msa0015900	MtrunA17_Chr2g0323411	57.265	117	20	1	1	87	1	117	1.07e-39	139
Msa0015900	MtrunA17_Chr4g0042671	59.483	116	17	1	1	86	1	116	3.42e-39	139

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0015900	AT3G23250.1	42.254	284	104	8	1	227	1	281	2.28e-58	186
Msa0015900	AT1G06180.1	43.542	271	87	10	1	230	1	246	4.86e-52	169
Msa0015900	AT2G31180.1	41.418	268	100	8	1	230	1	249	7.99e-52	168
Msa0015900	AT3G23250.2	42.982	228	98	8	28	227	1	224	3.50e-43	145
Msa0015900	AT1G16490.1	57.983	119	20	1	3	91	5	123	4.23e-42	144
Msa0015900	AT3G61250.1	45.402	174	55	3	1	134	1	174	6.42e-42	144
Msa0015900	AT1G79180.1	44.560	193	68	5	3	162	5	191	6.82e-42	144
Msa0015900	AT4G21440.1	58.120	117	19	1	1	87	1	117	2.01e-41	144
Msa0015900	AT1G79180.2	51.974	152	64	4	14	162	79	224	9.96e-41	142
Msa0015900	AT4G05100.1	58.475	118	18	2	1	87	1	118	4.21e-40	140

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Find 28 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCCAATTCCAACTCAA+TGG	0.490366	1_1:-26086996	Msa0015900:CDS
AAATTTCCTCTCTTAATATC+AGG	0.343705	1_1:+26087650	None:intergenic
AATTCATTTGAAACTGTCAT+AGG	0.356516	1_1:+26086794	None:intergenic
AGCTTCCATTGAGTTGGAAT+TGG	0.389117	1_1:+26086991	None:intergenic
ATGCAGGTTTATTGAGATGT+GGG	0.495585	1_1:-26087709	Msa0015900:intron
ATGCCATCGTCATTATCAAA+AGG	0.461830	1_1:+26086722	None:intergenic
ATTAAGATGCATGAGTTGCT+AGG	0.510133	1_1:-26087602	Msa0015900:CDS
ATTGTCACAAAAGAGTCTTC+AGG	0.488694	1_1:+26086890	None:intergenic
ATTTCATTGTCTGTTCTTCC+AGG	0.291634	1_1:+26087133	None:intergenic
CAAAAGCTTTCATCAATTTC+AGG	0.250594	1_1:+26086866	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAAGAAAGGATTGAAGAA+AGG	-	chr1_1:26086682-26086701	Msa0015900:CDS	30.0%
TTGAGAGACATTCTACTTTA+TGG	-	chr1_1:26086976-26086995	Msa0015900:CDS	30.0%
TTAAGACCTGATATTAAGAG+AGG	-	chr1_1:26087115-26087134	Msa0015900:CDS	30.0%
ACATGAAAATTACATGCGAA+TGG	-	chr1_1:26087317-26087336	Msa0015900:intron	30.0%
CAAAAGCTTTCATCAATTTC+AGG	+	chr1_1:26087908-26087927	None:intergenic	30.0%
TATAACGTGTTCATCAAATC+AGG	-	chr1_1:26088082-26088101	Msa0015900:intron	30.0%
GAAAAGAAAGGATTGAAGAA+AGG	-	chr1_1:26086682-26086701	Msa0015900:CDS	30.0%
TTGAGAGACATTCTACTTTA+TGG	-	chr1_1:26086976-26086995	Msa0015900:CDS	30.0%
TTAAGACCTGATATTAAGAG+AGG	-	chr1_1:26087115-26087134	Msa0015900:CDS	30.0%
ACATGAAAATTACATGCGAA+TGG	-	chr1_1:26087317-26087336	Msa0015900:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	26086661	26088132	26086661	ID=Msa0015900;Name=Msa0015900
chr1_1	mRNA	26086661	26088132	26086661	ID=Msa0015900-mRNA-1;Parent=Msa0015900;Name=Msa0015900-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0\|0\|0\|1\|1\|1\|3\|0\|230
chr1_1	exon	26088090	26088132	26088090	ID=Msa0015900-mRNA-1:exon:8913;Parent=Msa0015900-mRNA-1
chr1_1	exon	26087596	26087725	26087596	ID=Msa0015900-mRNA-1:exon:8912;Parent=Msa0015900-mRNA-1
chr1_1	exon	26086661	26087180	26086661	ID=Msa0015900-mRNA-1:exon:8911;Parent=Msa0015900-mRNA-1
chr1_1	CDS	26088090	26088132	26088090	ID=Msa0015900-mRNA-1:cds;Parent=Msa0015900-mRNA-1
chr1_1	CDS	26087596	26087725	26087596	ID=Msa0015900-mRNA-1:cds;Parent=Msa0015900-mRNA-1
chr1_1	CDS	26086661	26087180	26086661	ID=Msa0015900-mRNA-1:cds;Parent=Msa0015900-mRNA-1

Gene Sequence

>Msa0015900

ATGGTTAGAGCTCCATGTTGTGAAAAGAAAGGATTGAAGAAAGGTTTATTGAGATGTGGGAAGAGTTGTAGATTGAGATGGATTAATTATTTAAGACCTGATATTAAGAGAGGAAATTTTACAAATGAAGAAGAGGAAACTATCATTAAGATGCATGAGTTGCTAGGGAACAGATGGTCAGCAATAGCAGCAAAATTACCTGGAAGAACAGACAATGAAATAAAAAATGTATGGCACACTCACTTGAAGAAAAGACTACTGAATACCAAAAATAACCAACCTCATTCAAATACCAAAAAAAGAGTGTCAAAACAAAAAATCAAAAGATATGATTCAAATGCCAATTCCAACTCAATGGAAGCTACATCATCAAATTGCACTTTTTCTAGTAATTTTTCATCACAAGGAAGAAATGTTGACAACATCATCAATTGTGAAGACCCTGAAGACTCTTTTGTGACAATGCCTGAAATTGATGAAAGCTTTTGGTCAGAGAAAATAAATGATGACGAAATTAGCTCCACCATGCCATCAAATCCTATGACAGTTTCAAATGAATTACCAGATCAACAATATATTTTTAATAATTCAGTAGAAAATTTCCAAAACCCTTTTGATAATGACGATGGCATGGATTTTTGGTATAACGTGTTCATCAAATCAGGAGAATCAACAGAATTGCCAGAGTTCTAA

Protein sequence

>Msa0015900

MVRAPCCEKKGLKKGLLRCGKSCRLRWINYLRPDIKRGNFTNEEEETIIKMHELLGNRWSAIAAKLPGRTDNEIKNVWHTHLKKRLLNTKNNQPHSNTKKRVSKQKIKRYDSNANSNSMEATSSNCTFSSNFSSQGRNVDNIINCEDPEDSFVTMPEIDESFWSEKINDDEISSTMPSNPMTVSNELPDQQYIFNNSVENFQNPFDNDDGMDFWYNVFIKSGESTELPEF*