Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0016930 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_1:+28044519 | Msa0016930:CDS |
| CCACAAGCGTTACTTAGTAT+TGG | 0.413611 | 1_1:-28043910 | None:intergenic |
| GGACTATATTTCAAGGCTAA+AGG | 0.442562 | 1_1:+28043931 | Msa0016930:CDS |
| TGGAGATTGGTTTGAAAGCT+CGG | 0.447768 | 1_1:-28044037 | None:intergenic |
| CGCTTGTGGACTATATTTCA+AGG | 0.451874 | 1_1:+28043924 | Msa0016930:CDS |
| CTTAGTATTGGCTTCTCATA+CGG | 0.455064 | 1_1:-28043898 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1_1:+28043874 | Msa0016930:CDS |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1_1:+28043999 | Msa0016930:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_1:-28043957 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1_1:+28044608 | Msa0016930:CDS |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_1:+28044487 | Msa0016930:CDS |
| GGAGATTGGTTTGAAAGCTC+GGG | 0.493147 | 1_1:-28044036 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_1:+28044614 | Msa0016930:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_1:-28044485 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_1:+28044520 | Msa0016930:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_1:-28044614 | None:intergenic |
| GAGCTTTCAAACCAATCTCC+AGG | 0.568278 | 1_1:+28044039 | Msa0016930:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_1:-28043956 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_1:+28044490 | Msa0016930:CDS |
| CCAATACTAAGTAACGCTTG+TGG | 0.691772 | 1_1:+28043910 | Msa0016930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_1:28044099-28044118 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_1:28044100-28044119 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_1:28044459-28044478 | Msa0016930:intron | 15.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_1:28044099-28044118 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_1:28044100-28044119 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_1:28044459-28044478 | Msa0016930:intron | 15.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_1:28044060-28044079 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_1:28044101-28044120 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_1:28044154-28044173 | Msa0016930:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_1:28044205-28044224 | Msa0016930:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_1:28044298-28044317 | Msa0016930:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_1:28044299-28044318 | Msa0016930:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_1:28044439-28044458 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_1:28044060-28044079 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_1:28044101-28044120 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_1:28044154-28044173 | Msa0016930:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_1:28044205-28044224 | Msa0016930:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_1:28044298-28044317 | Msa0016930:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_1:28044299-28044318 | Msa0016930:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_1:28044439-28044458 | None:intergenic | 20.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_1:28043866-28043885 | Msa0016930:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_1:28044053-28044072 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_1:28044165-28044184 | Msa0016930:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_1:28044245-28044264 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_1:28044246-28044265 | None:intergenic | 25.0% |
| ! | ATTCATTCAAAACCTAATAC+CGG | - | chr1_1:28044290-28044309 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_1:28044300-28044319 | Msa0016930:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_1:28044348-28044367 | None:intergenic | 25.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_1:28043866-28043885 | Msa0016930:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_1:28044053-28044072 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_1:28044165-28044184 | Msa0016930:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_1:28044245-28044264 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_1:28044246-28044265 | None:intergenic | 25.0% |
| ! | ATTCATTCAAAACCTAATAC+CGG | - | chr1_1:28044290-28044309 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_1:28044300-28044319 | Msa0016930:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_1:28044348-28044367 | None:intergenic | 25.0% |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1_1:28043986-28044005 | None:intergenic | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_1:28044253-28044272 | None:intergenic | 30.0% | |
| TTCATTCAAAACCTAATACC+GGG | - | chr1_1:28044289-28044308 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_1:28044342-28044361 | Msa0016930:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_1:28044488-28044507 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_1:28044487-28044506 | Msa0016930:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_1:28044519-28044538 | Msa0016930:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_1:28044520-28044539 | Msa0016930:CDS | 30.0% | |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1_1:28043986-28044005 | None:intergenic | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_1:28044253-28044272 | None:intergenic | 30.0% | |
| TTCATTCAAAACCTAATACC+GGG | - | chr1_1:28044289-28044308 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_1:28044342-28044361 | Msa0016930:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_1:28044488-28044507 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_1:28044487-28044506 | Msa0016930:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_1:28044519-28044538 | Msa0016930:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_1:28044520-28044539 | Msa0016930:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_1:28043874-28043893 | Msa0016930:CDS | 35.0% | |
| !! | CTTAGTATTGGCTTCTCATA+CGG | - | chr1_1:28043901-28043920 | None:intergenic | 35.0% |
| GGACTATATTTCAAGGCTAA+AGG | + | chr1_1:28043931-28043950 | Msa0016930:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_1:28043959-28043978 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_1:28043960-28043979 | None:intergenic | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1_1:28043976-28043995 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1_1:28043987-28044006 | None:intergenic | 35.0% |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_1:28044306-28044325 | Msa0016930:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_1:28044426-28044445 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_1:28044490-28044509 | Msa0016930:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_1:28044608-28044627 | Msa0016930:CDS | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_1:28043874-28043893 | Msa0016930:CDS | 35.0% | |
| !! | CTTAGTATTGGCTTCTCATA+CGG | - | chr1_1:28043901-28043920 | None:intergenic | 35.0% |
| GGACTATATTTCAAGGCTAA+AGG | + | chr1_1:28043931-28043950 | Msa0016930:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_1:28043959-28043978 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_1:28043960-28043979 | None:intergenic | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1_1:28043976-28043995 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1_1:28043987-28044006 | None:intergenic | 35.0% |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_1:28044306-28044325 | Msa0016930:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_1:28044426-28044445 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_1:28044490-28044509 | Msa0016930:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_1:28044608-28044627 | Msa0016930:CDS | 35.0% | |
| CCACAAGCGTTACTTAGTAT+TGG | - | chr1_1:28043913-28043932 | None:intergenic | 40.0% | |
| CCAATACTAAGTAACGCTTG+TGG | + | chr1_1:28043910-28043929 | Msa0016930:CDS | 40.0% | |
| ! | CGCTTGTGGACTATATTTCA+AGG | + | chr1_1:28043924-28043943 | Msa0016930:CDS | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_1:28043999-28044018 | Msa0016930:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_1:28044008-28044027 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_1:28044040-28044059 | None:intergenic | 40.0% |
| AGCATTCATTTGAGTAGACC+CGG | + | chr1_1:28044268-28044287 | Msa0016930:intron | 40.0% | |
| ATTTGAGTAGACCCGGTATT+AGG | + | chr1_1:28044275-28044294 | Msa0016930:intron | 40.0% | |
| CCACAAGCGTTACTTAGTAT+TGG | - | chr1_1:28043913-28043932 | None:intergenic | 40.0% | |
| CCAATACTAAGTAACGCTTG+TGG | + | chr1_1:28043910-28043929 | Msa0016930:CDS | 40.0% | |
| ! | CGCTTGTGGACTATATTTCA+AGG | + | chr1_1:28043924-28043943 | Msa0016930:CDS | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_1:28043999-28044018 | Msa0016930:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_1:28044008-28044027 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_1:28044040-28044059 | None:intergenic | 40.0% |
| AGCATTCATTTGAGTAGACC+CGG | + | chr1_1:28044268-28044287 | Msa0016930:intron | 40.0% | |
| ATTTGAGTAGACCCGGTATT+AGG | + | chr1_1:28044275-28044294 | Msa0016930:intron | 40.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_1:28044039-28044058 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_1:28044039-28044058 | Msa0016930:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_1:28044617-28044636 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_1:28044614-28044633 | Msa0016930:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_1:28044039-28044058 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_1:28044039-28044058 | Msa0016930:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_1:28044617-28044636 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_1:28044614-28044633 | Msa0016930:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 28043862 | 28044682 | 28043862 | ID=Msa0016930;Name=Msa0016930 |
| chr1_1 | mRNA | 28043862 | 28044682 | 28043862 | ID=Msa0016930-mRNA-1;Parent=Msa0016930;Name=Msa0016930-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_1 | exon | 28043862 | 28044060 | 28043862 | ID=Msa0016930-mRNA-1:exon:9405;Parent=Msa0016930-mRNA-1 |
| chr1_1 | exon | 28044477 | 28044682 | 28044477 | ID=Msa0016930-mRNA-1:exon:9406;Parent=Msa0016930-mRNA-1 |
| chr1_1 | CDS | 28043862 | 28044060 | 28043862 | ID=Msa0016930-mRNA-1:cds;Parent=Msa0016930-mRNA-1 |
| chr1_1 | CDS | 28044477 | 28044682 | 28044477 | ID=Msa0016930-mRNA-1:cds;Parent=Msa0016930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
