AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0018680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0018680 MtrunA17_Chr1g0170581 85.656 244 35 0 1 244 1 244 2.08e-155 431
Msa0018680 MtrunA17_Chr1g0170591 86.475 244 25 1 1 244 1 236 1.22e-152 424
Msa0018680 MtrunA17_Chr1g0170571 82.520 246 35 1 1 246 1 238 8.84e-142 397
Msa0018680 MtrunA17_Chr7g0256671 56.175 251 91 6 1 241 1 242 4.83e-64 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0018680 AT3G56970.1 40.239 251 135 6 1 240 1 247 6.23e-52 169
Msa0018680 AT3G56980.1 39.560 273 115 6 1 242 1 254 6.75e-45 151
Msa0018680 AT2G41240.1 36.842 247 142 4 1 242 1 238 1.19e-41 142
Msa0018680 AT2G41240.2 36.437 247 142 5 1 242 1 237 3.80e-39 136
Msa0018680 AT5G04150.2 45.283 159 85 1 61 219 82 238 3.80e-35 125
Msa0018680 AT5G04150.1 44.068 177 94 3 65 239 86 259 8.64e-35 125

Find 39 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACATGTTTATGTATTTC+TGG 0.098207 1_1:-31643708 None:intergenic
TGATGCTGGTTATGGCTTAT+TGG 0.196697 1_1:-31643207 None:intergenic
AGTACCATTTGATCAGAAAT+AGG 0.267145 1_1:-31643426 None:intergenic
CAACTTCTCGTTTAAAATAT+CGG 0.270225 1_1:-31644137 None:intergenic
CGAGAAGTTGTTATCAATAA+TGG 0.272808 1_1:+31644150 Msa0018680:CDS
GTTTCTTCATCTCAAACCTT+TGG 0.309294 1_1:+31643969 Msa0018680:CDS
ATGGATTCTGATGCTGGTTA+TGG 0.357369 1_1:-31643215 None:intergenic
CATAGCAAAATCTCAGATAA+TGG 0.357726 1_1:-31643915 None:intergenic
AGGACTCTTGAAATTGTTGC+TGG 0.367920 1_1:-31643675 None:intergenic
TCAATAGTGTCTTTGTAGAA+TGG 0.399191 1_1:-31643234 None:intergenic
TCTTCCTATTTCTGATCAAA+TGG 0.404052 1_1:+31643422 Msa0018680:CDS
AACAATTGGATGGGCCTTTG+AGG 0.425119 1_1:+31643182 Msa0018680:CDS
TCTTCATCTCAAACCTTTGG+AGG 0.450222 1_1:+31643972 Msa0018680:CDS
TGTAGAATGGATTCTGATGC+TGG 0.456368 1_1:-31643221 None:intergenic
GTTGTTATCAATAATGGAAA+AGG 0.460271 1_1:+31644156 Msa0018680:CDS
AAGAAACATTTAGCAACATA+AGG 0.460296 1_1:-31643953 None:intergenic
TTGCTATGTTTAGAAAATGA+TGG 0.461428 1_1:+31643930 Msa0018680:CDS
ATCTCAAACCTTTGGAGGGA+GGG 0.471487 1_1:+31643977 Msa0018680:CDS
ATAATAACTTCATTTACTTG+TGG 0.512712 1_1:-31643774 None:intergenic
AAGAAACATAATTACAACTC+TGG 0.515926 1_1:+31643816 Msa0018680:CDS
ACAAATTATTCATCTTCTTG+CGG 0.523820 1_1:-31643380 None:intergenic
CTTCATCTCAAACCTTTGGA+GGG 0.524238 1_1:+31643973 Msa0018680:CDS
ATGGCTTATTGGCTCCTCAA+AGG 0.528875 1_1:-31643196 None:intergenic
CATGCAAAGGTCACTGCTTG+TGG 0.541878 1_1:-31643319 None:intergenic
CAATAATGGAAAAGGAAGGA+GGG 0.549179 1_1:+31644164 Msa0018680:CDS
CATCTCAAACCTTTGGAGGG+AGG 0.551061 1_1:+31643976 Msa0018680:CDS
TCAATAATGGAAAAGGAAGG+AGG 0.551798 1_1:+31644163 Msa0018680:CDS
CATCCAATTGTTGAAAACAT+AGG 0.558093 1_1:-31643171 None:intergenic
GTAGAAGACCCTCCCTCCAA+AGG 0.574235 1_1:-31643985 None:intergenic
CCAATTGTTGAAAACATAGG+AGG 0.582074 1_1:-31643168 None:intergenic
CACAAGCAGTGACCTTTGCA+TGG 0.598738 1_1:+31643320 Msa0018680:CDS
GAAATACATAAACATGTGGA+AGG 0.601655 1_1:+31643711 Msa0018680:CDS
TCTCAGATAATGGAATCTTG+TGG 0.603687 1_1:-31643905 None:intergenic
TTATCAATAATGGAAAAGGA+AGG 0.604075 1_1:+31644160 Msa0018680:CDS
CAGAAATAGGAAGAAGTGAA+CGG 0.605298 1_1:-31643413 None:intergenic
GTCACTGCTTGTGGATTGTG+AGG 0.612584 1_1:-31643310 None:intergenic
ACCAGAAATACATAAACATG+TGG 0.627789 1_1:+31643707 Msa0018680:CDS
CAAGCTTCTTGACCATGCAA+AGG 0.654337 1_1:-31643332 None:intergenic
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_1:-31643309 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAACTTCATTTACTTG+TGG - chr1_1:31643777-31643796 None:intergenic 20.0%
!!! AATTAATTAAAAGCTCCTTT+TGG + chr1_1:31644078-31644097 Msa0018680:intron 20.0%
!!! ATTAATTAAAAGCTCCTTTT+GGG + chr1_1:31644079-31644098 Msa0018680:intron 20.0%
!! ATAATAACTTCATTTACTTG+TGG - chr1_1:31643777-31643796 None:intergenic 20.0%
!!! AATTAATTAAAAGCTCCTTT+TGG + chr1_1:31644078-31644097 Msa0018680:intron 20.0%
!!! ATTAATTAAAAGCTCCTTTT+GGG + chr1_1:31644079-31644098 Msa0018680:intron 20.0%
!! TATGTTTTCAACAATTGGAT+GGG + chr1_1:31643173-31643192 Msa0018680:CDS 25.0%
! ACAAATTATTCATCTTCTTG+CGG - chr1_1:31643383-31643402 None:intergenic 25.0%
! ACAATTAGAAACAGAAAGAA+GGG - chr1_1:31643460-31643479 None:intergenic 25.0%
!! TCTAAAGTGATATCGAAAAA+CGG - chr1_1:31643576-31643595 None:intergenic 25.0%
!!! GTATTTCTGGTATGTATTTT+AGG - chr1_1:31643698-31643717 None:intergenic 25.0%
! AAGAAACATAATTACAACTC+TGG + chr1_1:31643816-31643835 Msa0018680:CDS 25.0%
! TTGCTATGTTTAGAAAATGA+TGG + chr1_1:31643930-31643949 Msa0018680:CDS 25.0%
! AAGAAACATTTAGCAACATA+AGG - chr1_1:31643956-31643975 None:intergenic 25.0%
!! TTTTCACAATGTGTAAATTC+AGG + chr1_1:31644051-31644070 Msa0018680:intron 25.0%
!! CAACTTCTCGTTTAAAATAT+CGG - chr1_1:31644140-31644159 None:intergenic 25.0%
!! GTTGTTATCAATAATGGAAA+AGG + chr1_1:31644156-31644175 Msa0018680:CDS 25.0%
!! TTATCAATAATGGAAAAGGA+AGG + chr1_1:31644160-31644179 Msa0018680:CDS 25.0%
!! TATGTTTTCAACAATTGGAT+GGG + chr1_1:31643173-31643192 Msa0018680:CDS 25.0%
! ACAAATTATTCATCTTCTTG+CGG - chr1_1:31643383-31643402 None:intergenic 25.0%
! ACAATTAGAAACAGAAAGAA+GGG - chr1_1:31643460-31643479 None:intergenic 25.0%
!! TCTAAAGTGATATCGAAAAA+CGG - chr1_1:31643576-31643595 None:intergenic 25.0%
!!! GTATTTCTGGTATGTATTTT+AGG - chr1_1:31643698-31643717 None:intergenic 25.0%
! AAGAAACATAATTACAACTC+TGG + chr1_1:31643816-31643835 Msa0018680:CDS 25.0%
! TTGCTATGTTTAGAAAATGA+TGG + chr1_1:31643930-31643949 Msa0018680:CDS 25.0%
! AAGAAACATTTAGCAACATA+AGG - chr1_1:31643956-31643975 None:intergenic 25.0%
!! TTTTCACAATGTGTAAATTC+AGG + chr1_1:31644051-31644070 Msa0018680:intron 25.0%
!! CAACTTCTCGTTTAAAATAT+CGG - chr1_1:31644140-31644159 None:intergenic 25.0%
!! GTTGTTATCAATAATGGAAA+AGG + chr1_1:31644156-31644175 Msa0018680:CDS 25.0%
!! TTATCAATAATGGAAAAGGA+AGG + chr1_1:31644160-31644179 Msa0018680:CDS 25.0%
CATCCAATTGTTGAAAACAT+AGG - chr1_1:31643174-31643193 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG + chr1_1:31643172-31643191 Msa0018680:CDS 30.0%
TCAATAGTGTCTTTGTAGAA+TGG - chr1_1:31643237-31643256 None:intergenic 30.0%
!! TCATTTTGATGATCAAGTAG+AGG + chr1_1:31643266-31643285 Msa0018680:CDS 30.0%
!! TCTTCCTATTTCTGATCAAA+TGG + chr1_1:31643422-31643441 Msa0018680:CDS 30.0%
AGTACCATTTGATCAGAAAT+AGG - chr1_1:31643429-31643448 None:intergenic 30.0%
GACAATTAGAAACAGAAAGA+AGG - chr1_1:31643461-31643480 None:intergenic 30.0%
!!! GTTTTGTTTTGACATGAGTA+TGG + chr1_1:31643528-31643547 Msa0018680:intron 30.0%
TATGGTTGGAATTTCCTAAT+AGG + chr1_1:31643546-31643565 Msa0018680:intron 30.0%
ATGGTTGGAATTTCCTAATA+GGG + chr1_1:31643547-31643566 Msa0018680:intron 30.0%
TTCGATATCACTTTAGATTG+TGG + chr1_1:31643579-31643598 Msa0018680:intron 30.0%
ACCAGAAATACATAAACATG+TGG + chr1_1:31643707-31643726 Msa0018680:CDS 30.0%
TCCACATGTTTATGTATTTC+TGG - chr1_1:31643711-31643730 None:intergenic 30.0%
GAAATACATAAACATGTGGA+AGG + chr1_1:31643711-31643730 Msa0018680:CDS 30.0%
! GTTTTATCGTTTCAACAAGT+AGG + chr1_1:31643838-31643857 Msa0018680:CDS 30.0%
CATAGCAAAATCTCAGATAA+TGG - chr1_1:31643918-31643937 None:intergenic 30.0%
! CTTCTACAATTTGCATTTTC+AGG + chr1_1:31644001-31644020 Msa0018680:CDS 30.0%
CGAGAAGTTGTTATCAATAA+TGG + chr1_1:31644150-31644169 Msa0018680:CDS 30.0%
CATCCAATTGTTGAAAACAT+AGG - chr1_1:31643174-31643193 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG + chr1_1:31643172-31643191 Msa0018680:CDS 30.0%
TCAATAGTGTCTTTGTAGAA+TGG - chr1_1:31643237-31643256 None:intergenic 30.0%
!! TCATTTTGATGATCAAGTAG+AGG + chr1_1:31643266-31643285 Msa0018680:CDS 30.0%
!! TCTTCCTATTTCTGATCAAA+TGG + chr1_1:31643422-31643441 Msa0018680:CDS 30.0%
AGTACCATTTGATCAGAAAT+AGG - chr1_1:31643429-31643448 None:intergenic 30.0%
GACAATTAGAAACAGAAAGA+AGG - chr1_1:31643461-31643480 None:intergenic 30.0%
!!! GTTTTGTTTTGACATGAGTA+TGG + chr1_1:31643528-31643547 Msa0018680:intron 30.0%
TATGGTTGGAATTTCCTAAT+AGG + chr1_1:31643546-31643565 Msa0018680:intron 30.0%
ATGGTTGGAATTTCCTAATA+GGG + chr1_1:31643547-31643566 Msa0018680:intron 30.0%
TTCGATATCACTTTAGATTG+TGG + chr1_1:31643579-31643598 Msa0018680:intron 30.0%
ACCAGAAATACATAAACATG+TGG + chr1_1:31643707-31643726 Msa0018680:CDS 30.0%
TCCACATGTTTATGTATTTC+TGG - chr1_1:31643711-31643730 None:intergenic 30.0%
GAAATACATAAACATGTGGA+AGG + chr1_1:31643711-31643730 Msa0018680:CDS 30.0%
! GTTTTATCGTTTCAACAAGT+AGG + chr1_1:31643838-31643857 Msa0018680:CDS 30.0%
CATAGCAAAATCTCAGATAA+TGG - chr1_1:31643918-31643937 None:intergenic 30.0%
! CTTCTACAATTTGCATTTTC+AGG + chr1_1:31644001-31644020 Msa0018680:CDS 30.0%
CGAGAAGTTGTTATCAATAA+TGG + chr1_1:31644150-31644169 Msa0018680:CDS 30.0%
CCAATTGTTGAAAACATAGG+AGG - chr1_1:31643171-31643190 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG + chr1_1:31643168-31643187 Msa0018680:CDS 35.0%
CAGAAATAGGAAGAAGTGAA+CGG - chr1_1:31643416-31643435 None:intergenic 35.0%
!!! TGTTTTGACATGAGTATGGT+TGG + chr1_1:31643532-31643551 Msa0018680:intron 35.0%
!! TCTCAGATAATGGAATCTTG+TGG - chr1_1:31643908-31643927 None:intergenic 35.0%
GTTTCTTCATCTCAAACCTT+TGG + chr1_1:31643969-31643988 Msa0018680:CDS 35.0%
! TCAATAATGGAAAAGGAAGG+AGG + chr1_1:31644163-31644182 Msa0018680:CDS 35.0%
! CAATAATGGAAAAGGAAGGA+GGG + chr1_1:31644164-31644183 Msa0018680:CDS 35.0%
CCAATTGTTGAAAACATAGG+AGG - chr1_1:31643171-31643190 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG + chr1_1:31643168-31643187 Msa0018680:CDS 35.0%
CAGAAATAGGAAGAAGTGAA+CGG - chr1_1:31643416-31643435 None:intergenic 35.0%
!!! TGTTTTGACATGAGTATGGT+TGG + chr1_1:31643532-31643551 Msa0018680:intron 35.0%
!! TCTCAGATAATGGAATCTTG+TGG - chr1_1:31643908-31643927 None:intergenic 35.0%
GTTTCTTCATCTCAAACCTT+TGG + chr1_1:31643969-31643988 Msa0018680:CDS 35.0%
! TCAATAATGGAAAAGGAAGG+AGG + chr1_1:31644163-31644182 Msa0018680:CDS 35.0%
! CAATAATGGAAAAGGAAGGA+GGG + chr1_1:31644164-31644183 Msa0018680:CDS 35.0%
! TGATGCTGGTTATGGCTTAT+TGG - chr1_1:31643210-31643229 None:intergenic 40.0%
! ATGGATTCTGATGCTGGTTA+TGG - chr1_1:31643218-31643237 None:intergenic 40.0%
! TGTAGAATGGATTCTGATGC+TGG - chr1_1:31643224-31643243 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG - chr1_1:31643678-31643697 None:intergenic 40.0%
TCTTCATCTCAAACCTTTGG+AGG + chr1_1:31643972-31643991 Msa0018680:CDS 40.0%
CTTCATCTCAAACCTTTGGA+GGG + chr1_1:31643973-31643992 Msa0018680:CDS 40.0%
! TGATGCTGGTTATGGCTTAT+TGG - chr1_1:31643210-31643229 None:intergenic 40.0%
! ATGGATTCTGATGCTGGTTA+TGG - chr1_1:31643218-31643237 None:intergenic 40.0%
! TGTAGAATGGATTCTGATGC+TGG - chr1_1:31643224-31643243 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG - chr1_1:31643678-31643697 None:intergenic 40.0%
TCTTCATCTCAAACCTTTGG+AGG + chr1_1:31643972-31643991 Msa0018680:CDS 40.0%
CTTCATCTCAAACCTTTGGA+GGG + chr1_1:31643973-31643992 Msa0018680:CDS 40.0%
AACAATTGGATGGGCCTTTG+AGG + chr1_1:31643182-31643201 Msa0018680:CDS 45.0%
!! ATGGCTTATTGGCTCCTCAA+AGG - chr1_1:31643199-31643218 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG - chr1_1:31643312-31643331 None:intergenic 45.0%
CAAGCTTCTTGACCATGCAA+AGG - chr1_1:31643335-31643354 None:intergenic 45.0%
CGAAAAACGGCATCCCTATT+AGG - chr1_1:31643563-31643582 None:intergenic 45.0%
! ATCTCAAACCTTTGGAGGGA+GGG + chr1_1:31643977-31643996 Msa0018680:CDS 45.0%
AACAATTGGATGGGCCTTTG+AGG + chr1_1:31643182-31643201 Msa0018680:CDS 45.0%
!! ATGGCTTATTGGCTCCTCAA+AGG - chr1_1:31643199-31643218 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG - chr1_1:31643312-31643331 None:intergenic 45.0%
CAAGCTTCTTGACCATGCAA+AGG - chr1_1:31643335-31643354 None:intergenic 45.0%
CGAAAAACGGCATCCCTATT+AGG - chr1_1:31643563-31643582 None:intergenic 45.0%
! ATCTCAAACCTTTGGAGGGA+GGG + chr1_1:31643977-31643996 Msa0018680:CDS 45.0%
! GTCACTGCTTGTGGATTGTG+AGG - chr1_1:31643313-31643332 None:intergenic 50.0%
CATGCAAAGGTCACTGCTTG+TGG - chr1_1:31643322-31643341 None:intergenic 50.0%
CACAAGCAGTGACCTTTGCA+TGG + chr1_1:31643320-31643339 Msa0018680:CDS 50.0%
! CATCTCAAACCTTTGGAGGG+AGG + chr1_1:31643976-31643995 Msa0018680:CDS 50.0%
!!! GCTCCTTTTGGGTTTTCTGC+AGG + chr1_1:31644090-31644109 Msa0018680:intron 50.0%
CCACCTGCAGAAAACCCAAA+AGG - chr1_1:31644096-31644115 None:intergenic 50.0%
!!! CCTTTTGGGTTTTCTGCAGG+TGG + chr1_1:31644093-31644112 Msa0018680:intron 50.0%
! GTCACTGCTTGTGGATTGTG+AGG - chr1_1:31643313-31643332 None:intergenic 50.0%
CATGCAAAGGTCACTGCTTG+TGG - chr1_1:31643322-31643341 None:intergenic 50.0%
CACAAGCAGTGACCTTTGCA+TGG + chr1_1:31643320-31643339 Msa0018680:CDS 50.0%
! CATCTCAAACCTTTGGAGGG+AGG + chr1_1:31643976-31643995 Msa0018680:CDS 50.0%
!!! GCTCCTTTTGGGTTTTCTGC+AGG + chr1_1:31644090-31644109 Msa0018680:intron 50.0%
CCACCTGCAGAAAACCCAAA+AGG - chr1_1:31644096-31644115 None:intergenic 50.0%
!!! CCTTTTGGGTTTTCTGCAGG+TGG + chr1_1:31644093-31644112 Msa0018680:intron 50.0%
GTAGAAGACCCTCCCTCCAA+AGG - chr1_1:31643988-31644007 None:intergenic 55.0%
GTAGAAGACCCTCCCTCCAA+AGG - chr1_1:31643988-31644007 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 31643153 31644201 31643153 ID=Msa0018680;Name=Msa0018680
chr1_1 mRNA 31643153 31644201 31643153 ID=Msa0018680-mRNA-1;Parent=Msa0018680;Name=Msa0018680-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|3|0|247
chr1_1 exon 31643153 31643443 31643153 ID=Msa0018680-mRNA-1:exon:10381;Parent=Msa0018680-mRNA-1
chr1_1 exon 31643660 31644022 31643660 ID=Msa0018680-mRNA-1:exon:10382;Parent=Msa0018680-mRNA-1
chr1_1 exon 31644112 31644201 31644112 ID=Msa0018680-mRNA-1:exon:10383;Parent=Msa0018680-mRNA-1
chr1_1 CDS 31643153 31643443 31643153 ID=Msa0018680-mRNA-1:cds;Parent=Msa0018680-mRNA-1
chr1_1 CDS 31643660 31644022 31643660 ID=Msa0018680-mRNA-1:cds;Parent=Msa0018680-mRNA-1
chr1_1 CDS 31644112 31644201 31644112 ID=Msa0018680-mRNA-1:cds;Parent=Msa0018680-mRNA-1
Gene Sequence

>Msa0018680

ATGTTAGCGATATCTCCTCCTATGTTTTCAACAATTGGATGGGCCTTTGAGGAGCCAATAAGCCATAACCAGCATCAGAATCCATTCTACAAAGACACTATTGATCAATTATTTCATTTTGATGATCAAGTAGAGGCAGAAATTAATTCAACAGATCCCTCACAATCCACAAGCAGTGACCTTTGCATGGTCAAGAAGCTTGTTCATAATGCTAGTGAACGTCATCGCCGCAAGAAGATGAATAATTTGTATTCTTCACTCCGTTCACTTCTTCCTATTTCTGATCAAATGAAGAAATTGAGTATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAAATACATAAACATGTGGAAGGACTAATTGAGAGAAAAGAAGAGATCTTATTGAGACTTTCTCCACAAGTAAATGAAGTTATTATAAGCAAAGAATCTCAAAAGAAGAAACATAATTACAACTCTGGTTTTATCGTTTCAACAAGTAGGCTAAATGATAGTGAAGTTGCAATTCAGATTTCATGTTACACTGTCCACAAGATTCCATTATCTGAGATTTTGCTATGTTTAGAAAATGATGGCCTTATGTTGCTAAATGTTTCTTCATCTCAAACCTTTGGAGGGAGGGTCTTCTACAATTTGCATTTTCAGGTGGATAAAATACAAAGATTAGAATCCGATATTTTAAACGAGAAGTTGTTATCAATAATGGAAAAGGAAGGAGGGTTTTCAATACAATAA

Protein sequence

>Msa0018680

MLAISPPMFSTIGWAFEEPISHNQHQNPFYKDTIDQLFHFDDQVEAEINSTDPSQSTSSDLCMVKKLVHNASERHRRKKMNNLYSSLRSLLPISDQMKKLSIPATISRVLKYIPEIHKHVEGLIERKEEILLRLSPQVNEVIISKESQKKKHNYNSGFIVSTSRLNDSEVAIQISCYTVHKIPLSEILLCLENDGLMLLNVSSSQTFGGRVFYNLHFQVDKIQRLESDILNEKLLSIMEKEGGFSIQ*